Rw4G030980

Methyladenine glycosylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0007725
Physical Location & Seq
Forward (+)
57783269 .. 57787187
3919 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G030980.1

Sequence Viewer

Length: 1017 bp
ATGATCACCATTCAAAGGTGGAAGAAGGAGACACAGCTTCATTTCTTTGTCCTCTGCAACATGTCTAAAGCAAATGTAAGAAGACAATTAGTACTTCTGGAGAAGAACAAGGTTCCCAAGGAGAAGAGTACTACTAGTGCTTTCAGCTCCCACTTTTCTTATAAACACCTAAAGAGAATCTACCCAATTGGGCTTCACAAGAGTTCTTCATCTTCCTCTCTATCTCTATCATCCTTATCTTTGTCTTTGTCAGAAAACTCCATTGACTCTTCTTCTATCATTGATTCTACTTCTCCACTCGATCAGAAGATTTCATCAGCACTCCGCCTCATTGCACCGCCGCCCCAAAGAAGAGAGTCTCCGGTGCCTAAAGTTGTCCAACAGCAGAATCAGAGCTTCCAGGATACTGATAATGGGGAGTTGAGGAGGTGCAATTGGATAACAAAGAACAGCGATAAAGTTTATGTTGCATTTCATGATGAATGCTGGGGAGTTCCAGTATATGATGACAATCAATTGTTTGAGCTGCTTGCCCTGTCTGGTATGTTGATGGACCACAATTGGACTGAAATTGTGAAAAGAAGGGAGCTATTCAGGGAAGCCTTTTCTGGATTTGATCCAAACACAGTTGCCAAAATGGGGGAGGAGGAGATTGAAGAGATAGCTTCCAACAAAGCTTTAATGTTGCCAGACTGCAAAGTCAGGTGCATTGTAGACAATGCCAAATGCATATTGAAGATTGTGAGGGAATGTGGATCTTTCAGTAGCTATATGTGGGGTTCTGTGAATCACAAACCAGTGATCAACAGGTACAGACACCCGAGAAATGTTCCTCTGAGGAGCCCGAAAGCAGAAGCCATGAGCAAGGATTTGATAAAGCGGGGATTTCGATACGTTGGACCAGTCATTGTGTACTCATTCATGCAGGCTGCTGGGTTGACCATCGATCATCTTGTGGATTGCTATAGGTATAGTGAGTGTGTAAGCCTTGCAGAAAGACCTTGGAGACATATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03410 map03410
Pfam Domains
Protein Families
Adenine_glyco

Protein Analysis

338

Amino Acids

38.76

Weight (kDa)

9.19

Isoelectric Point (pI)

60.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adenine_glyco PF03352 149 - 323 1.5e-65 Methyladenine glycosylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 162
AasI GACNNNNNNGTC 1 cut(s) 698
AccB1I GGYRCC 1 cut(s) 364
AccI GTMKAC 1 cut(s) 714
AciI CCGC 4 cut(s) 325, 338, 341, 880
AclWI GGATC 2 cut(s) 611, 763
AfaI GTAC 4 cut(s) 93, 130, 812, 914
AfiI CCNNNNNNNGG 2 cut(s) 15, 639
AflIII ACRYGT 1 cut(s) 60
AgsI TTSAA 3 cut(s) 14, 656, 736
AhlI ACTAGT 1 cut(s) 134
AjnI CCWGG 1 cut(s) 399
AluBI AGCT 8 cut(s) 37, 147, 396, 526, 589, 665, 677, 768
AluI AGCT 8 cut(s) 37, 147, 396, 526, 589, 665, 677, 768
Alw26I GTCTC 3 cut(s) 23, 363, 1000
AlwI GGATC 2 cut(s) 611, 763
Ama87I CYCGRG 1 cut(s) 820
ApeKI GCWGC 2 cut(s) 526, 929
AspS9I GGNCC 2 cut(s) 553, 899
AvaI CYCGRG 1 cut(s) 820
AvaII GGWCC 2 cut(s) 553, 899
BanI GGYRCC 1 cut(s) 364
BanII GRGCYC 1 cut(s) 845
BbsI GAAGAC 1 cut(s) 88
BbvI GCAGC 2 cut(s) 513, 916
BccI CCATC 2 cut(s) 544, 950
BciT130I CCWGG 1 cut(s) 401
BciVI GTATCC 1 cut(s) 397
BclI TGATCA 2 cut(s) 3, 801
BcoDI GTCTC 3 cut(s) 23, 363, 1000
BcuI ACTAGT 1 cut(s) 134
BfaI CTAG 2 cut(s) 135, 1015
BfmI CTRYAG 1 cut(s) 964
BfuI GTATCC 1 cut(s) 397
BisI GCNGC 3 cut(s) 341, 527, 930
BlsI GCNGC 3 cut(s) 342, 528, 931
BmcAI AGTACT 2 cut(s) 93, 130
Bme1390I CCNGG 1 cut(s) 401
Bme18I GGWCC 2 cut(s) 553, 899
BmeT110I CYCGRG 1 cut(s) 820
BmgT120I GGNCC 2 cut(s) 553, 899
BmiI GGNNCC 3 cut(s) 114, 366, 842
BmrFI CCNGG 1 cut(s) 401
BpiI GAAGAC 1 cut(s) 88
BpmI CTGGAG 1 cut(s) 119
Bsa29I ATCGAT 1 cut(s) 945
BsaBI GATNNNNATC 1 cut(s) 510
BsaJI CCNNGG 2 cut(s) 117, 1001
BsaWI WCCGGW 1 cut(s) 361
BsaXI ACNNNNNCTCC 2 cut(s) 483, 513
Bsc4I CCNNNNNNNGG 2 cut(s) 15, 639
Bse1I ACTGG 3 cut(s) 497, 797, 902
Bse3DI GCAATG 1 cut(s) 330
Bse8I GATNNNNATC 1 cut(s) 510
BseBI CCWGG 1 cut(s) 401
BseCI ATCGAT 1 cut(s) 945
BseDI CCNNGG 2 cut(s) 117, 1001
BseGI GGATG 1 cut(s) 230
BseJI GATNNNNATC 1 cut(s) 510
BseLI CCNNNNNNNGG 2 cut(s) 15, 639
BseMI GCAATG 1 cut(s) 330
BseMII CTCAG 1 cut(s) 827
BseNI ACTGG 3 cut(s) 497, 797, 902
BseRI GAGGAG 4 cut(s) 439, 659, 662, 853
BseXI GCAGC 2 cut(s) 513, 916
BseYI CCCAGC 2 cut(s) 486, 932
BshNI GGYRCC 1 cut(s) 364
BshVI ATCGAT 1 cut(s) 945
BsiHKCI CYCGRG 1 cut(s) 820
BsiSI CCGG 1 cut(s) 362
BslI CCNNNNNNNGG 2 cut(s) 15, 639
BsmAI GTCTC 3 cut(s) 23, 363, 1000
BsmI GAATGC 1 cut(s) 488
BsoBI CYCGRG 1 cut(s) 820
Bsp1286I GDGCHC 1 cut(s) 845
Bsp143I GATC 6 cut(s) 3, 301, 616, 755, 801, 946
BspACI CCGC 4 cut(s) 325, 338, 341, 880
BspCNI CTCAG 1 cut(s) 828
BspDI ATCGAT 1 cut(s) 945
BspHI TCATGA 1 cut(s) 475
BspLI GGNNCC 3 cut(s) 114, 366, 842
BspPI GGATC 2 cut(s) 611, 763
BspT107I GGYRCC 1 cut(s) 364
BsrDI GCAATG 1 cut(s) 330
BsrI ACTGG 3 cut(s) 497, 797, 902
BssECI CCNNGG 2 cut(s) 117, 1001
BssMI GATC 6 cut(s) 3, 301, 616, 755, 801, 946
BssT1I CCWWGG 2 cut(s) 117, 1001
Bst2UI CCWGG 1 cut(s) 401
Bst4CI ACNGT 1 cut(s) 628
Bst6I CTCTTC 4 cut(s) 119, 274, 346, 651
BstC8I GCNNGC 2 cut(s) 531, 927
BstDEI CTNAG 1 cut(s) 836
BstF5I GGATG 1 cut(s) 230
BstKTI GATC 6 cut(s) 6, 304, 619, 758, 804, 949
BstMAI GTCTC 3 cut(s) 23, 363, 1000
BstMBI GATC 6 cut(s) 3, 301, 616, 755, 801, 946
BstNI CCWGG 1 cut(s) 401
BstNSI RCATGY 1 cut(s) 64
BstSCI CCNGG 1 cut(s) 399
BstSFI CTRYAG 1 cut(s) 964
BstV1I GCAGC 2 cut(s) 513, 916
BstV2I GAAGAC 1 cut(s) 88
BstX2I RGATCY 1 cut(s) 755
BstYI RGATCY 1 cut(s) 755
Bsu15I ATCGAT 1 cut(s) 945
BsuI GTATCC 1 cut(s) 397
BsuTUI ATCGAT 1 cut(s) 945
BtsCI GGATG 1 cut(s) 230
BtsIMutI CAGTG 1 cut(s) 804
Cac8I GCNNGC 2 cut(s) 531, 927
CciI TCATGA 1 cut(s) 475
Cfr13I GGNCC 2 cut(s) 553, 899
ClaI ATCGAT 1 cut(s) 945
Csp6I GTAC 4 cut(s) 92, 129, 811, 913
CviAII CATG 4 cut(s) 61, 476, 859, 922
CviQI GTAC 4 cut(s) 92, 129, 811, 913
DdeI CTNAG 1 cut(s) 836
DpnI GATC 6 cut(s) 5, 303, 618, 757, 803, 948
DpnII GATC 6 cut(s) 3, 301, 616, 755, 801, 946
DrdI GACNNNNNNGTC 1 cut(s) 698
DseDI GACNNNNNNGTC 1 cut(s) 698
Eam1104I CTCTTC 4 cut(s) 119, 274, 346, 651
EarI CTCTTC 4 cut(s) 119, 274, 346, 651
EciI GGCGGA 1 cut(s) 314
Eco130I CCWWGG 2 cut(s) 117, 1001
Eco24I GRGCYC 1 cut(s) 845
Eco47I GGWCC 2 cut(s) 553, 899
Eco88I CYCGRG 1 cut(s) 820
EcoRII CCWGG 1 cut(s) 399
EcoT14I CCWWGG 2 cut(s) 117, 1001
EcoT22I ATGCAT 1 cut(s) 731
EcoT38I GRGCYC 1 cut(s) 845
ErhI CCWWGG 2 cut(s) 117, 1001
FaeI CATG 4 cut(s) 64, 479, 862, 925
FatI CATG 4 cut(s) 60, 475, 858, 921
FauI CCCGC 1 cut(s) 873
FbaI TGATCA 2 cut(s) 3, 801
FblI GTMKAC 1 cut(s) 714
Fnu4HI GCNGC 3 cut(s) 341, 527, 930
FokI GGATG 1 cut(s) 217
FriOI GRGCYC 1 cut(s) 845
Fsp4HI GCNGC 3 cut(s) 341, 527, 930
FspBI CTAG 2 cut(s) 135, 1015
GluI GCNGC 3 cut(s) 341, 527, 930
GsaI CCCAGC 2 cut(s) 490, 936
GsuI CTGGAG 1 cut(s) 119
HapII CCGG 1 cut(s) 362
Hin1II CATG 4 cut(s) 64, 479, 862, 925
HincII GTYRAC 1 cut(s) 939
HindII GTYRAC 1 cut(s) 939
HindIII AAGCTT 1 cut(s) 675
HinfI GANTC 6 cut(s) 177, 266, 284, 356, 388, 787
HpaII CCGG 1 cut(s) 362
Hpy166II GTNNAC 3 cut(s) 715, 913, 939
Hpy188I TCNGA 4 cut(s) 253, 306, 393, 837
Hpy188III TCNNGA 3 cut(s) 98, 476, 609
Hpy8I GTNNAC 3 cut(s) 715, 913, 939
HpyAV CCTTC 2 cut(s) 19, 576
HpyCH4III ACNGT 1 cut(s) 628
HpyCH4IV ACGT 1 cut(s) 894
HpyCH4V TGCA 9 cut(s) 57, 335, 432, 470, 696, 708, 729, 925, 992
HpyF3I CTNAG 1 cut(s) 836
HpySE526I ACGT 1 cut(s) 894
Hsp92II CATG 4 cut(s) 64, 479, 862, 925
Ksp22I TGATCA 2 cut(s) 3, 801
Kzo9I GATC 6 cut(s) 3, 301, 616, 755, 801, 946
LmnI GCTCC 3 cut(s) 152, 586, 840
Lsp1109I GCAGC 2 cut(s) 513, 916
MaeI CTAG 2 cut(s) 135, 1015
MaeII ACGT 1 cut(s) 894
MalI GATC 6 cut(s) 5, 303, 618, 757, 803, 948
MboI GATC 6 cut(s) 3, 301, 616, 755, 801, 946
MfeI CAATTG 4 cut(s) 186, 433, 515, 559
MflI RGATCY 1 cut(s) 755
MhlI GDGCHC 1 cut(s) 845
MluCI AATT 6 cut(s) 86, 186, 433, 515, 559, 570
MlyI GAGTC 2 cut(s) 260, 365
MmeI TCCRAC 3 cut(s) 403, 693, 877
Mph1103I ATGCAT 1 cut(s) 731
MseI TTAA 1 cut(s) 680
MspI CCGG 1 cut(s) 362
MspR9I CCNGG 1 cut(s) 401
MunI CAATTG 4 cut(s) 186, 433, 515, 559
Mva1269I GAATGC 1 cut(s) 488
MvaI CCWGG 1 cut(s) 401
NdeII GATC 6 cut(s) 3, 301, 616, 755, 801, 946
NlaIII CATG 4 cut(s) 64, 479, 862, 925
NlaIV GGNNCC 3 cut(s) 114, 366, 842
NsiI ATGCAT 1 cut(s) 731
NspI RCATGY 1 cut(s) 64
PagI TCATGA 1 cut(s) 475
PciI ACATGT 1 cut(s) 60
PctI GAATGC 1 cut(s) 488
PfeI GAWTC 4 cut(s) 177, 284, 388, 787
PfoI TCCNGGA 1 cut(s) 399
PkrI GCNGC 3 cut(s) 342, 528, 931
PleI GAGTC 2 cut(s) 260, 364
PpsI GAGTC 2 cut(s) 260, 364
PscI ACATGT 1 cut(s) 60
PsiI TTATAA 1 cut(s) 162
Psp6I CCWGG 1 cut(s) 399
PspFI CCCAGC 2 cut(s) 486, 932
PspGI CCWGG 1 cut(s) 399
PspN4I GGNNCC 3 cut(s) 114, 366, 842
PspPI GGNCC 2 cut(s) 553, 899
PsuI RGATCY 1 cut(s) 755
RsaI GTAC 4 cut(s) 93, 130, 812, 914
RsaNI GTAC 4 cut(s) 92, 129, 811, 913
SaqAI TTAA 1 cut(s) 680
SatI GCNGC 3 cut(s) 341, 527, 930
Sau3AI GATC 6 cut(s) 3, 301, 616, 755, 801, 946
Sau96I GGNCC 2 cut(s) 553, 899
ScaI AGTACT 2 cut(s) 93, 130
SchI GAGTC 2 cut(s) 260, 365
ScrFI CCNGG 1 cut(s) 401
SduI GDGCHC 1 cut(s) 845
SfcI CTRYAG 1 cut(s) 964
SinI GGWCC 2 cut(s) 553, 899
SpeI ACTAGT 1 cut(s) 134
Sse9I AATT 6 cut(s) 86, 186, 433, 515, 559, 570
SsiI CCGC 4 cut(s) 325, 338, 341, 880
SspMI CTAG 2 cut(s) 135, 1015
StyD4I CCNGG 1 cut(s) 399
StyI CCWWGG 2 cut(s) 117, 1001
TaaI ACNGT 1 cut(s) 628
TaiI ACGT 1 cut(s) 897
TaqI TCGA 3 cut(s) 300, 889, 945
TasI AATT 6 cut(s) 86, 186, 433, 515, 559, 570
TatI WGTACW 3 cut(s) 91, 128, 912
TauI GCSGC 1 cut(s) 343
TfiI GAWTC 4 cut(s) 177, 284, 388, 787
Tru1I TTAA 1 cut(s) 680
Tru9I TTAA 1 cut(s) 680
TscAI CASTG 1 cut(s) 804
TseI GCWGC 2 cut(s) 526, 929
TspDTI ATGAA 6 cut(s) 29, 198, 303, 464, 495, 910
TspRI CASTG 1 cut(s) 804
VpaK11BI GGWCC 2 cut(s) 553, 899
XceI RCATGY 1 cut(s) 64
XmiI GTMKAC 1 cut(s) 714
XspI CTAG 2 cut(s) 135, 1015
ZrmI AGTACT 2 cut(s) 93, 130
Zsp2I ATGCAT 1 cut(s) 731
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.