Rroxscaffold_5G00378330

Methyladenine glycosylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0007725
Physical Location & Seq
Forward (+)
58784128 .. 58786673
2546 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00378330.1

Sequence Viewer

Length: 957 bp
ATGTCTAAAGCAAATGTAAGAAGACAATTAGTACTTCTGGAGAAGAACAAGGTTCCCAAGGAGAAGAGTACTACTAGTGCTTTCAGCTCCCACTTTTCTTATAAACACCTAAAGAGAATCTACCCAATTGGGCTTCACAAGAGTTCTTCATCTTCCTCTCTATCTCTGTCATCCTTATCCTTGTCTTTGTCAGAAAACTCCATTGACTCTTCTTCTATCATTGATTCTACTTCTCCACTCGATCAGAAGATTTCATCAGCACTCCGCCTCATTGCACCGCCGGCCCAAAGAAGAGAGTCTCCGGTGCCTAAAGTTGTCCAACAGCAGAATCAGAACTTTCAGGATACTGATAATGGGGAGCTGAGGAGGTGCAATTGGATAACAAAGAATAGCGATAAAGTTTATGTTGCATTTCATGATGAATGCTGGGGAGTTCCAGTATATGATGACAATCAATTGTTTGAGCTGCTTGCACTGTCTGGTATGTTGATGGACCACAATTGGACTGAAATTGTGAAAAGAAGGGAGCTATTCAGGGAAGCCTTTTCTGGATTTGATCCAAACACAGTTGCCAAAATGGGGGAGGAGGAGATTGAAGAGATAGCTTCCAACAAAGCTTTAATGTTGCCAGACTGCAAAGTCAGGTGCATTGTAGACAATGCCAAATGCATATTGAAGATTGTGAGGGAATGTGGATCTTTCAGTAGCTATATGTGGGGTTCTGTGAATCACAAACCAGTGATCAATAGGTTCAGACACCCGAGAAATGTTCCTCTGAGGAGCCCGAAAGCAGAAGCCATGAGCAAGGATTTGATAAAGCGGGGATTTCGATACGTTGGACCAGTCATTGTGTACTCATTCATGCAGGCTGCTGGGTTGACCATCGATCATCTTGTGGATTGCTATAGATATAGTGAGTGTGTAAGCCTTGCAGAAAGACCTTGGAGACATATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03410 map03410
Pfam Domains
Protein Families
Adenine_glyco

Protein Analysis

318

Amino Acids

36.23

Weight (kDa)

9.11

Isoelectric Point (pI)

60.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adenine_glyco PF03352 129 - 303 1.4e-65 Methyladenine glycosylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 102
AasI GACNNNNNNGTC 1 cut(s) 638
AccB1I GGYRCC 1 cut(s) 304
AccI GTMKAC 1 cut(s) 654
AciI CCGC 3 cut(s) 265, 278, 820
AclWI GGATC 2 cut(s) 551, 703
AfaI GTAC 3 cut(s) 33, 70, 854
AfiI CCNNNNNNNGG 1 cut(s) 579
AgsI TTSAA 2 cut(s) 596, 676
AhlI ACTAGT 1 cut(s) 74
AluBI AGCT 7 cut(s) 87, 361, 466, 529, 605, 617, 708
AluI AGCT 7 cut(s) 87, 361, 466, 529, 605, 617, 708
Alw26I GTCTC 2 cut(s) 303, 940
AlwI GGATC 2 cut(s) 551, 703
Ama87I CYCGRG 1 cut(s) 760
AoxI GGCC 1 cut(s) 282
ApeKI GCWGC 2 cut(s) 466, 869
AspS9I GGNCC 3 cut(s) 283, 493, 839
AvaI CYCGRG 1 cut(s) 760
AvaII GGWCC 2 cut(s) 493, 839
BanI GGYRCC 1 cut(s) 304
BanII GRGCYC 1 cut(s) 785
BbsI GAAGAC 1 cut(s) 28
BbvCI CCTCAGC 1 cut(s) 362
BbvI GCAGC 2 cut(s) 453, 856
BccI CCATC 2 cut(s) 484, 890
BciVI GTATCC 1 cut(s) 337
BclI TGATCA 1 cut(s) 741
BcoDI GTCTC 2 cut(s) 303, 940
BcuI ACTAGT 1 cut(s) 74
BfaI CTAG 2 cut(s) 75, 955
BfmI CTRYAG 1 cut(s) 904
BfuI GTATCC 1 cut(s) 337
BisI GCNGC 2 cut(s) 467, 870
BlsI GCNGC 2 cut(s) 468, 871
BmcAI AGTACT 2 cut(s) 33, 70
Bme18I GGWCC 2 cut(s) 493, 839
BmeT110I CYCGRG 1 cut(s) 760
BmgT120I GGNCC 3 cut(s) 283, 493, 839
BmiI GGNNCC 3 cut(s) 54, 306, 782
BpiI GAAGAC 1 cut(s) 28
BpmI CTGGAG 1 cut(s) 59
Bpu10I CCTNAGC 1 cut(s) 362
Bsa29I ATCGAT 1 cut(s) 885
BsaBI GATNNNNATC 1 cut(s) 450
BsaJI CCNNGG 2 cut(s) 57, 941
BsaWI WCCGGW 1 cut(s) 301
BsaXI ACNNNNNCTCC 2 cut(s) 423, 453
Bsc4I CCNNNNNNNGG 1 cut(s) 579
Bse118I RCCGGY 1 cut(s) 280
Bse1I ACTGG 3 cut(s) 437, 737, 842
Bse3DI GCAATG 1 cut(s) 270
Bse8I GATNNNNATC 1 cut(s) 450
BseCI ATCGAT 1 cut(s) 885
BseDI CCNNGG 2 cut(s) 57, 941
BseGI GGATG 1 cut(s) 170
BseJI GATNNNNATC 1 cut(s) 450
BseLI CCNNNNNNNGG 1 cut(s) 579
BseMI GCAATG 1 cut(s) 270
BseMII CTCAG 2 cut(s) 353, 767
BseNI ACTGG 3 cut(s) 437, 737, 842
BseRI GAGGAG 4 cut(s) 379, 599, 602, 793
BseXI GCAGC 2 cut(s) 453, 856
BseYI CCCAGC 2 cut(s) 426, 872
BshFI GGCC 1 cut(s) 284
BshNI GGYRCC 1 cut(s) 304
BshVI ATCGAT 1 cut(s) 885
BsiHKCI CYCGRG 1 cut(s) 760
BsiSI CCGG 2 cut(s) 281, 302
BslI CCNNNNNNNGG 1 cut(s) 579
BsmAI GTCTC 2 cut(s) 303, 940
BsmI GAATGC 1 cut(s) 428
BsnI GGCC 1 cut(s) 284
BsoBI CYCGRG 1 cut(s) 760
Bsp1286I GDGCHC 1 cut(s) 785
Bsp143I GATC 5 cut(s) 241, 556, 695, 741, 886
BspACI CCGC 3 cut(s) 265, 278, 820
BspANI GGCC 1 cut(s) 284
BspCNI CTCAG 2 cut(s) 354, 768
BspDI ATCGAT 1 cut(s) 885
BspHI TCATGA 1 cut(s) 415
BspLI GGNNCC 3 cut(s) 54, 306, 782
BspPI GGATC 2 cut(s) 551, 703
BspT107I GGYRCC 1 cut(s) 304
BsrDI GCAATG 1 cut(s) 270
BsrFI RCCGGY 1 cut(s) 280
BsrI ACTGG 3 cut(s) 437, 737, 842
BssAI RCCGGY 1 cut(s) 280
BssECI CCNNGG 2 cut(s) 57, 941
BssMI GATC 5 cut(s) 241, 556, 695, 741, 886
BssT1I CCWWGG 2 cut(s) 57, 941
Bst4CI ACNGT 2 cut(s) 477, 568
Bst6I CTCTTC 4 cut(s) 59, 214, 286, 591
BstC8I GCNNGC 3 cut(s) 282, 471, 867
BstDEI CTNAG 2 cut(s) 362, 776
BstF5I GGATG 1 cut(s) 170
BstKTI GATC 5 cut(s) 244, 559, 698, 744, 889
BstMAI GTCTC 2 cut(s) 303, 940
BstMBI GATC 5 cut(s) 241, 556, 695, 741, 886
BstMWI GCNNNNNNNGC 1 cut(s) 281
BstSFI CTRYAG 1 cut(s) 904
BstV1I GCAGC 2 cut(s) 453, 856
BstV2I GAAGAC 1 cut(s) 28
BstX2I RGATCY 1 cut(s) 695
BstYI RGATCY 1 cut(s) 695
Bsu15I ATCGAT 1 cut(s) 885
BsuI GTATCC 1 cut(s) 337
BsuRI GGCC 1 cut(s) 284
BsuTUI ATCGAT 1 cut(s) 885
BtsCI GGATG 1 cut(s) 170
BtsIMutI CAGTG 2 cut(s) 473, 744
Cac8I GCNNGC 3 cut(s) 282, 471, 867
CciI TCATGA 1 cut(s) 415
Cfr10I RCCGGY 1 cut(s) 280
Cfr13I GGNCC 3 cut(s) 283, 493, 839
ClaI ATCGAT 1 cut(s) 885
Csp6I GTAC 3 cut(s) 32, 69, 853
CviAII CATG 3 cut(s) 416, 799, 862
CviQI GTAC 3 cut(s) 32, 69, 853
DdeI CTNAG 2 cut(s) 362, 776
DpnI GATC 5 cut(s) 243, 558, 697, 743, 888
DpnII GATC 5 cut(s) 241, 556, 695, 741, 886
DrdI GACNNNNNNGTC 1 cut(s) 638
DseDI GACNNNNNNGTC 1 cut(s) 638
Eam1104I CTCTTC 4 cut(s) 59, 214, 286, 591
EarI CTCTTC 4 cut(s) 59, 214, 286, 591
EciI GGCGGA 1 cut(s) 254
Eco130I CCWWGG 2 cut(s) 57, 941
Eco24I GRGCYC 1 cut(s) 785
Eco47I GGWCC 2 cut(s) 493, 839
Eco88I CYCGRG 1 cut(s) 760
EcoT14I CCWWGG 2 cut(s) 57, 941
EcoT22I ATGCAT 1 cut(s) 671
EcoT38I GRGCYC 1 cut(s) 785
ErhI CCWWGG 2 cut(s) 57, 941
FaeI CATG 3 cut(s) 419, 802, 865
FatI CATG 3 cut(s) 415, 798, 861
FauI CCCGC 1 cut(s) 813
FbaI TGATCA 1 cut(s) 741
FblI GTMKAC 1 cut(s) 654
Fnu4HI GCNGC 2 cut(s) 467, 870
FokI GGATG 1 cut(s) 157
FriOI GRGCYC 1 cut(s) 785
Fsp4HI GCNGC 2 cut(s) 467, 870
FspBI CTAG 2 cut(s) 75, 955
GluI GCNGC 2 cut(s) 467, 870
GsaI CCCAGC 2 cut(s) 430, 876
GsuI CTGGAG 1 cut(s) 59
HaeIII GGCC 1 cut(s) 284
HapII CCGG 2 cut(s) 281, 302
Hin1II CATG 3 cut(s) 419, 802, 865
HincII GTYRAC 1 cut(s) 879
HindII GTYRAC 1 cut(s) 879
HindIII AAGCTT 1 cut(s) 615
HinfI GANTC 6 cut(s) 117, 206, 224, 296, 328, 727
HpaII CCGG 2 cut(s) 281, 302
Hpy166II GTNNAC 3 cut(s) 655, 853, 879
Hpy188I TCNGA 5 cut(s) 193, 246, 333, 755, 777
Hpy188III TCNNGA 4 cut(s) 38, 341, 416, 549
Hpy8I GTNNAC 3 cut(s) 655, 853, 879
HpyAV CCTTC 1 cut(s) 516
HpyCH4III ACNGT 2 cut(s) 477, 568
HpyCH4IV ACGT 1 cut(s) 834
HpyCH4V TGCA 9 cut(s) 275, 372, 410, 473, 636, 648, 669, 865, 932
HpyF10VI GCNNNNNNNGC 1 cut(s) 281
HpyF3I CTNAG 2 cut(s) 362, 776
HpySE526I ACGT 1 cut(s) 834
Hsp92II CATG 3 cut(s) 419, 802, 865
KroI GCCGGC 1 cut(s) 280
KroNI GCCGGC 1 cut(s) 282
Ksp22I TGATCA 1 cut(s) 741
Kzo9I GATC 5 cut(s) 241, 556, 695, 741, 886
LmnI GCTCC 4 cut(s) 92, 358, 526, 780
Lsp1109I GCAGC 2 cut(s) 453, 856
MaeI CTAG 2 cut(s) 75, 955
MaeII ACGT 1 cut(s) 834
MalI GATC 5 cut(s) 243, 558, 697, 743, 888
MboI GATC 5 cut(s) 241, 556, 695, 741, 886
MfeI CAATTG 4 cut(s) 126, 373, 455, 499
MflI RGATCY 1 cut(s) 695
MhlI GDGCHC 1 cut(s) 785
MluCI AATT 6 cut(s) 26, 126, 373, 455, 499, 510
MlyI GAGTC 2 cut(s) 200, 305
MmeI TCCRAC 3 cut(s) 343, 633, 817
MnlI CCTC 9 cut(s) 166, 278, 357, 360, 577, 580, 678, 771, 783
Mph1103I ATGCAT 1 cut(s) 671
MroNI GCCGGC 1 cut(s) 280
MseI TTAA 1 cut(s) 620
MspI CCGG 2 cut(s) 281, 302
MunI CAATTG 4 cut(s) 126, 373, 455, 499
Mva1269I GAATGC 1 cut(s) 428
MwoI GCNNNNNNNGC 1 cut(s) 281
NaeI GCCGGC 1 cut(s) 282
NdeII GATC 5 cut(s) 241, 556, 695, 741, 886
NgoMIV GCCGGC 1 cut(s) 280
NlaIII CATG 3 cut(s) 419, 802, 865
NlaIV GGNNCC 3 cut(s) 54, 306, 782
NsiI ATGCAT 1 cut(s) 671
PagI TCATGA 1 cut(s) 415
PctI GAATGC 1 cut(s) 428
PdiI GCCGGC 1 cut(s) 282
PfeI GAWTC 4 cut(s) 117, 224, 328, 727
PkrI GCNGC 2 cut(s) 468, 871
PleI GAGTC 2 cut(s) 200, 304
PpsI GAGTC 2 cut(s) 200, 304
PsiI TTATAA 1 cut(s) 102
PspFI CCCAGC 2 cut(s) 426, 872
PspN4I GGNNCC 3 cut(s) 54, 306, 782
PspPI GGNCC 3 cut(s) 283, 493, 839
PsuI RGATCY 1 cut(s) 695
RsaI GTAC 3 cut(s) 33, 70, 854
RsaNI GTAC 3 cut(s) 32, 69, 853
SaqAI TTAA 1 cut(s) 620
SatI GCNGC 2 cut(s) 467, 870
Sau3AI GATC 5 cut(s) 241, 556, 695, 741, 886
Sau96I GGNCC 3 cut(s) 283, 493, 839
ScaI AGTACT 2 cut(s) 33, 70
SchI GAGTC 2 cut(s) 200, 305
SduI GDGCHC 1 cut(s) 785
SfcI CTRYAG 1 cut(s) 904
SinI GGWCC 2 cut(s) 493, 839
SpeI ACTAGT 1 cut(s) 74
Sse9I AATT 6 cut(s) 26, 126, 373, 455, 499, 510
SsiI CCGC 3 cut(s) 265, 278, 820
SspMI CTAG 2 cut(s) 75, 955
StyI CCWWGG 2 cut(s) 57, 941
TaaI ACNGT 2 cut(s) 477, 568
TaiI ACGT 1 cut(s) 837
TaqI TCGA 3 cut(s) 240, 829, 885
TasI AATT 6 cut(s) 26, 126, 373, 455, 499, 510
TatI WGTACW 3 cut(s) 31, 68, 852
TfiI GAWTC 4 cut(s) 117, 224, 328, 727
Tru1I TTAA 1 cut(s) 620
Tru9I TTAA 1 cut(s) 620
TscAI CASTG 2 cut(s) 480, 744
TseI GCWGC 2 cut(s) 466, 869
TspDTI ATGAA 5 cut(s) 138, 243, 404, 435, 850
TspRI CASTG 2 cut(s) 480, 744
VpaK11BI GGWCC 2 cut(s) 493, 839
XmiI GTMKAC 1 cut(s) 654
XspI CTAG 2 cut(s) 75, 955
ZrmI AGTACT 2 cut(s) 33, 70
Zsp2I ATGCAT 1 cut(s) 671
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.