FvH4_4g30130

hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0006490
Physical Location & Seq
Forward (+)
29919833 .. 29922674
2842 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g30130.t3

Sequence Viewer

Length: 999 bp
ATGATATCCCTCACAGCCATGGGAGCCACTCCCACATTCCAAAGAGGCCTCACTGTTTGCTGCTCCGACGCATTCCCTTCTTTCCTCCCCAAACAAGTCCACCACATCAAAGACTCATCTGCCCGGAAACTCGCCGCACGTATCGAGAGGCTCCCGGTGAGTTTTGGTGAGGACTGTGTAATGAGCAGCTGCGTGAAGCCGTTGGTACACAGGGAGACGAGCCCGGTGGTTCTTCTGCATGGTTTTGATAGCTCATGTTTAGAATGGAGATACACTTATCCGTTGCTTGAGGAAGCCGGCCTCGAGACTTGGGCTGTTGACGTTCTTGGTTGGGGTTTCTCTGATTTAGAAAGACTTCCTTCATGTAGCGTGGCCTCGAAGCGTGACCATTTGTTTCAGCTTTGGAAGTCCTACATTAAAAAGCCGATGGTATTGGTTGGACCAAGCCTTGGTGCTGCTGTTGCTATTGACTTTGCAACCAGCTATCCAGAAGCAGTTGAAAGGCTAGTTTTGATTGATGCAAGTGTGTATACAGAAGGCACTGAAAACCAACCAACCTTGTCTAAAATGTTAGCCTATTTTGGAGCATTTATATTGAAGAGTTTCCCTTTGCGCTTTTATGTCAACTTTTTGTGCTTTTTCACTGACATACCGTTGAGTACCAAAGTAGACTGGACAAATATTGGCCGCTTGCATTGCTTGTTTCCTTGGTGGGAGGATGCACTTGTGGATTTCATGATGAGTGGGGTGTATAATGTTAGTGCCCAGATAGAACAGGTAAAGCAGAGGACACTTATCATATGGGGTGAGGAAGACCAGATTATTAGCAACAAGCTTGGAGTGAGATTGCACTCTGAGCTGCCAAATGCTATCATACGACAAATACCAGATTGCGGACACCTTCCTCATGTAGAAAGACCGAGCTCTGTTGTGAAATTGATTGTGGAATTTGTTCAAGAAGCCAAGTGCAAAGAGGTTGAATATACTTCTCAGTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6 Hydrolase_4

Protein Analysis

333

Amino Acids

37.45

Weight (kDa)

5.87

Isoelectric Point (pI)

39.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 6 - 122 3.4e-07 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 6 - 228 3e-12 Alpha/beta hydrolase family
Hydrolase_4 PF12146 13 - 107 1.6e-07 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 170 - 224 6e-06 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 449
AccI GTMKAC 2 cut(s) 530, 669
AciI CCGC 3 cut(s) 135, 688, 894
AcoI YGGCCR 1 cut(s) 685
AcsI RAATTY 1 cut(s) 947
AfaI GTAC 2 cut(s) 207, 661
AfiI CCNNNNNNNGG 2 cut(s) 449, 893
AgsI TTSAA 4 cut(s) 500, 598, 956, 980
AluBI AGCT 7 cut(s) 189, 252, 400, 483, 835, 859, 924
AluI AGCT 7 cut(s) 189, 252, 400, 483, 835, 859, 924
Alw21I GWGCWC 1 cut(s) 926
Alw26I GTCTC 2 cut(s) 209, 299
Ama87I CYCGRG 1 cut(s) 302
AoxI GGCC 4 cut(s) 46, 298, 372, 685
ApeKI GCWGC 5 cut(s) 60, 186, 189, 455, 859
ApoI RAATTY 1 cut(s) 947
Asp700I GAANNNNTTC 3 cut(s) 354, 602, 951
AspLEI GCGC 1 cut(s) 615
AspS9I GGNCC 1 cut(s) 440
AsuC2I CCSGG 3 cut(s) 124, 155, 224
AsuHPI GGTGA 3 cut(s) 169, 179, 818
AvaI CYCGRG 1 cut(s) 302
AvaII GGWCC 1 cut(s) 440
BaeGI GKGCMC 1 cut(s) 766
BanII GRGCYC 2 cut(s) 224, 926
BbsI GAAGAC 1 cut(s) 819
Bbv12I GWGCWC 1 cut(s) 926
BbvI GCAGC 5 cut(s) 47, 176, 198, 442, 846
BccI CCATC 1 cut(s) 421
BceAI ACGGC 1 cut(s) 184
BcnI CCSGG 3 cut(s) 124, 155, 224
BcoDI GTCTC 2 cut(s) 209, 299
BfaI CTAG 1 cut(s) 506
BisI GCNGC 7 cut(s) 61, 135, 187, 190, 456, 688, 860
BlsI GCNGC 7 cut(s) 62, 136, 188, 191, 457, 689, 861
Bme1390I CCNGG 3 cut(s) 124, 155, 224
Bme18I GGWCC 1 cut(s) 440
BmeT110I CYCGRG 1 cut(s) 302
BmgT120I GGNCC 1 cut(s) 440
BmiI GGNNCC 2 cut(s) 25, 152
BmrFI CCNGG 3 cut(s) 124, 155, 224
BmsI GCATC 2 cut(s) 508, 709
BpiI GAAGAC 1 cut(s) 819
BpuEI CTTGAG 1 cut(s) 308
BpuMI CCSGG 3 cut(s) 124, 155, 224
BsaAI YACGTR 1 cut(s) 140
BsaJI CCNNGG 3 cut(s) 18, 448, 707
Bsc4I CCNNNNNNNGG 2 cut(s) 449, 893
Bse118I RCCGGY 1 cut(s) 296
Bse1I ACTGG 1 cut(s) 677
Bse3DI GCAATG 1 cut(s) 694
BseDI CCNNGG 3 cut(s) 18, 448, 707
BseGI GGATG 1 cut(s) 724
BseLI CCNNNNNNNGG 2 cut(s) 449, 893
BseMI GCAATG 1 cut(s) 694
BseMII CTCAG 1 cut(s) 846
BseNI ACTGG 1 cut(s) 677
BseSI GKGCMC 1 cut(s) 766
BseXI GCAGC 5 cut(s) 47, 176, 198, 442, 846
BshFI GGCC 4 cut(s) 48, 300, 374, 687
BsiHKAI GWGCWC 1 cut(s) 926
BsiHKCI CYCGRG 1 cut(s) 302
BsiSI CCGG 4 cut(s) 124, 155, 224, 297
BslI CCNNNNNNNGG 2 cut(s) 449, 893
BsmAI GTCTC 2 cut(s) 209, 299
BsmBI CGTCTC 1 cut(s) 209
BsmI GAATGC 1 cut(s) 71
BsnI GGCC 4 cut(s) 48, 300, 374, 687
BsoBI CYCGRG 1 cut(s) 302
Bsp1286I GDGCHC 3 cut(s) 224, 766, 926
Bsp19I CCATGG 1 cut(s) 18
BspACI CCGC 3 cut(s) 135, 688, 894
BspANI GGCC 4 cut(s) 48, 300, 374, 687
BspCNI CTCAG 1 cut(s) 847
BspHI TCATGA 1 cut(s) 735
BspLI GGNNCC 2 cut(s) 25, 152
BsrDI GCAATG 1 cut(s) 694
BsrFI RCCGGY 1 cut(s) 296
BsrI ACTGG 1 cut(s) 677
BssAI RCCGGY 1 cut(s) 296
BssECI CCNNGG 3 cut(s) 18, 448, 707
BssNAI GTATAC 1 cut(s) 531
BssT1I CCWWGG 3 cut(s) 18, 448, 707
Bst1107I GTATAC 1 cut(s) 531
Bst4CI ACNGT 3 cut(s) 55, 176, 654
Bst6I CTCTTC 1 cut(s) 593
BstBAI YACGTR 1 cut(s) 140
BstC8I GCNNGC 2 cut(s) 298, 692
BstDEI CTNAG 2 cut(s) 855, 990
BstDSI CCRYGG 1 cut(s) 18
BstF5I GGATG 1 cut(s) 724
BstHHI GCGC 1 cut(s) 615
BstMAI GTCTC 2 cut(s) 209, 299
BstMWI GCNNNNNNNGC 4 cut(s) 23, 461, 696, 856
BstSCI CCNGG 3 cut(s) 122, 153, 222
BstSLI GKGCMC 1 cut(s) 766
BstV1I GCAGC 5 cut(s) 47, 176, 198, 442, 846
BstV2I GAAGAC 1 cut(s) 819
BstZ17I GTATAC 1 cut(s) 531
BsuRI GGCC 4 cut(s) 48, 300, 374, 687
BtgI CCRYGG 1 cut(s) 18
BtsCI GGATG 1 cut(s) 724
BtsIMutI CAGTG 3 cut(s) 51, 540, 642
Cac8I GCNNGC 2 cut(s) 298, 692
CciI TCATGA 1 cut(s) 735
CfoI GCGC 1 cut(s) 615
Cfr10I RCCGGY 1 cut(s) 296
Cfr13I GGNCC 1 cut(s) 440
CseI GACGC 1 cut(s) 77
Csp6I GTAC 2 cut(s) 206, 660
CviAII CATG 6 cut(s) 19, 239, 255, 363, 736, 908
CviQI GTAC 2 cut(s) 206, 660
DdeI CTNAG 2 cut(s) 855, 990
EaeI YGGCCR 1 cut(s) 685
Eam1104I CTCTTC 1 cut(s) 593
EarI CTCTTC 1 cut(s) 593
Ecl136II GAGCTC 1 cut(s) 924
Eco130I CCWWGG 3 cut(s) 18, 448, 707
Eco147I AGGCCT 1 cut(s) 48
Eco24I GRGCYC 2 cut(s) 224, 926
Eco32I GATATC 1 cut(s) 6
Eco47I GGWCC 1 cut(s) 440
Eco53kI GAGCTC 1 cut(s) 924
Eco88I CYCGRG 1 cut(s) 302
EcoICRI GAGCTC 1 cut(s) 924
EcoRV GATATC 1 cut(s) 6
EcoT14I CCWWGG 3 cut(s) 18, 448, 707
EcoT38I GRGCYC 2 cut(s) 224, 926
ErhI CCWWGG 3 cut(s) 18, 448, 707
Esp3I CGTCTC 1 cut(s) 209
FaeI CATG 6 cut(s) 22, 242, 258, 366, 739, 911
FalI AAGNNNNNCTT 2 cut(s) 343, 375
FatI CATG 6 cut(s) 18, 238, 254, 362, 735, 907
FauNDI CATATG 1 cut(s) 800
FblI GTMKAC 2 cut(s) 530, 669
Fnu4HI GCNGC 7 cut(s) 61, 135, 187, 190, 456, 688, 860
FokI GGATG 1 cut(s) 731
FriOI GRGCYC 2 cut(s) 224, 926
Fsp4HI GCNGC 7 cut(s) 61, 135, 187, 190, 456, 688, 860
FspBI CTAG 1 cut(s) 506
GlaI GCGC 1 cut(s) 614
GluI GCNGC 7 cut(s) 61, 135, 187, 190, 456, 688, 860
HaeIII GGCC 4 cut(s) 48, 300, 374, 687
HapII CCGG 4 cut(s) 124, 155, 224, 297
HgaI GACGC 1 cut(s) 77
HhaI GCGC 1 cut(s) 615
Hin1II CATG 6 cut(s) 22, 242, 258, 366, 739, 911
Hin6I GCGC 1 cut(s) 613
HinP1I GCGC 1 cut(s) 613
HincII GTYRAC 2 cut(s) 319, 625
HindII GTYRAC 2 cut(s) 319, 625
HindIII AAGCTT 1 cut(s) 833
HinfI GANTC 1 cut(s) 113
HpaII CCGG 4 cut(s) 124, 155, 224, 297
HphI GGTGA 3 cut(s) 169, 179, 818
Hpy166II GTNNAC 6 cut(s) 100, 208, 319, 531, 625, 670
Hpy188I TCNGA 3 cut(s) 67, 343, 856
Hpy188III TCNNGA 5 cut(s) 145, 304, 488, 736, 956
Hpy8I GTNNAC 6 cut(s) 100, 208, 319, 531, 625, 670
Hpy99I CGWCG 1 cut(s) 71
HpyAV CCTTC 4 cut(s) 87, 369, 530, 911
HpyCH4III ACNGT 3 cut(s) 55, 176, 654
HpyCH4IV ACGT 2 cut(s) 139, 321
HpyCH4V TGCA 7 cut(s) 238, 476, 521, 694, 722, 850, 969
HpyF10VI GCNNNNNNNGC 4 cut(s) 23, 461, 696, 856
HpyF3I CTNAG 2 cut(s) 855, 990
HpySE526I ACGT 2 cut(s) 139, 321
Hsp92II CATG 6 cut(s) 22, 242, 258, 366, 739, 911
HspAI GCGC 1 cut(s) 613
KroI GCCGGC 1 cut(s) 296
KroNI GCCGGC 1 cut(s) 298
LmnI GCTCC 4 cut(s) 23, 68, 156, 584
Lsp1109I GCAGC 5 cut(s) 47, 176, 198, 442, 846
LweI GCATC 2 cut(s) 508, 709
MaeI CTAG 1 cut(s) 506
MaeII ACGT 2 cut(s) 139, 321
MaeIII GTNAC 1 cut(s) 383
MboII GAAGA 3 cut(s) 224, 610, 824
MhlI GDGCHC 3 cut(s) 224, 766, 926
MluCI AATT 2 cut(s) 935, 947
MlyI GAGTC 1 cut(s) 107
MmeI TCCRAC 2 cut(s) 90, 418
MroNI GCCGGC 1 cut(s) 296
MroXI GAANNNNTTC 3 cut(s) 354, 602, 951
MseI TTAA 1 cut(s) 417
MslI CAYNNNNRTG 1 cut(s) 17
MspA1I CMGCKG 1 cut(s) 189
MspI CCGG 4 cut(s) 124, 155, 224, 297
MspR9I CCNGG 3 cut(s) 124, 155, 224
Mva1269I GAATGC 1 cut(s) 71
MwoI GCNNNNNNNGC 4 cut(s) 23, 461, 696, 856
NaeI GCCGGC 1 cut(s) 298
NciI CCSGG 3 cut(s) 124, 155, 224
NcoI CCATGG 1 cut(s) 18
NdeI CATATG 1 cut(s) 800
NgoMIV GCCGGC 1 cut(s) 296
NlaIII CATG 6 cut(s) 22, 242, 258, 366, 739, 911
NlaIV GGNNCC 2 cut(s) 25, 152
NmuCI GTSAC 1 cut(s) 383
PaeR7I CTCGAG 1 cut(s) 302
PagI TCATGA 1 cut(s) 735
PceI AGGCCT 1 cut(s) 48
PctI GAATGC 1 cut(s) 71
PdiI GCCGGC 1 cut(s) 298
PdmI GAANNNNTTC 3 cut(s) 354, 602, 951
PflMI CCANNNNNTGG 1 cut(s) 449
PkrI GCNGC 7 cut(s) 62, 136, 188, 191, 457, 689, 861
PleI GAGTC 1 cut(s) 107
PpsI GAGTC 1 cut(s) 107
Ppu21I YACGTR 1 cut(s) 140
Psp124BI GAGCTC 1 cut(s) 926
PspN4I GGNNCC 2 cut(s) 25, 152
PspPI GGNCC 1 cut(s) 440
PvuII CAGCTG 1 cut(s) 189
RsaI GTAC 2 cut(s) 207, 661
RsaNI GTAC 2 cut(s) 206, 660
RseI CAYNNNNRTG 1 cut(s) 17
SacI GAGCTC 1 cut(s) 926
SaqAI TTAA 1 cut(s) 417
SatI GCNGC 7 cut(s) 61, 135, 187, 190, 456, 688, 860
Sau96I GGNCC 1 cut(s) 440
SchI GAGTC 1 cut(s) 107
ScrFI CCNGG 3 cut(s) 124, 155, 224
SduI GDGCHC 3 cut(s) 224, 766, 926
SfaNI GCATC 2 cut(s) 508, 709
Sfr274I CTCGAG 1 cut(s) 302
SinI GGWCC 1 cut(s) 440
SlaI CTCGAG 1 cut(s) 302
SmiMI CAYNNNNRTG 1 cut(s) 17
SmlI CTYRAG 2 cut(s) 287, 302
SmoI CTYRAG 2 cut(s) 287, 302
Sse9I AATT 2 cut(s) 935, 947
SseBI AGGCCT 1 cut(s) 48
SsiI CCGC 3 cut(s) 135, 688, 894
SspI AATATT 1 cut(s) 682
SspMI CTAG 1 cut(s) 506
SstI GAGCTC 1 cut(s) 926
StuI AGGCCT 1 cut(s) 48
StyD4I CCNGG 3 cut(s) 122, 153, 222
StyI CCWWGG 3 cut(s) 18, 448, 707
TaaI ACNGT 3 cut(s) 55, 176, 654
TaiI ACGT 2 cut(s) 142, 324
TaqI TCGA 3 cut(s) 144, 303, 377
TaqII GACCGA 1 cut(s) 934
TasI AATT 2 cut(s) 935, 947
TauI GCSGC 2 cut(s) 137, 690
Tru1I TTAA 1 cut(s) 417
Tru9I TTAA 1 cut(s) 417
TscAI CASTG 3 cut(s) 58, 547, 649
TseFI GTSAC 1 cut(s) 383
TseI GCWGC 5 cut(s) 60, 186, 189, 455, 859
Tsp45I GTSAC 1 cut(s) 383
TspDTI ATGAA 2 cut(s) 351, 724
TspGWI ACGGA 1 cut(s) 270
TspRI CASTG 3 cut(s) 58, 547, 649
Van91I CCANNNNNTGG 1 cut(s) 449
VpaK11BI GGWCC 1 cut(s) 440
XapI RAATTY 1 cut(s) 947
XhoI CTCGAG 1 cut(s) 302
XmiI GTMKAC 2 cut(s) 530, 669
XmnI GAANNNNTTC 3 cut(s) 354, 602, 951
XspI CTAG 1 cut(s) 506
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.