Rmu_sc0002327.1_g000021

hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002327.1
OG0006490
Physical Location & Seq
Reverse (-)
64310 .. 66387
2078 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002327.1_g000021.1.cds

Sequence Viewer

Length: 873 bp
atgttaacactcacagccagctctctccccattcacaccgcctccacagtccaaaggggtctcactgttcgctccgatgcattcccttctttccttcccaaagaggtcgagaacatcaaagacccatttgcccggaaactcgctacacgaatcgaaaggcttcccgttagttttggtgagaattgtgttatgagtagctgtgtgaagccgttggttcggagcgagacgactccggtggttcttctccatggttttgacagctcctgtttagaatggagatacacttatccattgcttgaggaagccggcctcgagacttgggctgttgacattcttggttggggtttctctgatttagaaagactccccccatgtaatgtggcctcgaagcgtgaccatttttttcagctttggaagtcctacattaaaaagccaatgatattggttggaccaagcctcggtgctgctgttgcaattgactttgcagccagctatccagaagcagttgaaaggctagttttgattgatgcaagtgtgtatacagaaggcaccgaaaatcaaccaaccttgtctaaaatgttagcctatgccggagcatatatattgaagagtttccctttgcgcttctatgtaacctctttgtgcttcactggtatatcattgagtaccagcctagattggacaaatattggccgcttgcattgctggtttccttggtgggaggatgcgcttgtggatttcatgatgagtggggtttacaatgttagtgcccagatagaacaggtaaagcagaggacacttatcatatggggtgaggaagaccagatcattagcaacaagcttggagtggtagtacttttctcctttctatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6 Hydrolase_4

Protein Analysis

290

Amino Acids

32.59

Weight (kDa)

5.48

Isoelectric Point (pI)

41.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 548
AccI GTMKAC 1 cut(s) 539
AciI CCGC 2 cut(s) 39, 694
AcoI YGGCCR 1 cut(s) 691
AfaI GTAC 2 cut(s) 667, 855
AfiI CCNNNNNNNGG 1 cut(s) 458
AgsI TTSAA 2 cut(s) 509, 607
AluBI AGCT 6 cut(s) 21, 198, 261, 409, 492, 841
AluI AGCT 6 cut(s) 21, 198, 261, 409, 492, 841
Alw26I GTCTC 3 cut(s) 65, 218, 308
AlwNI CAGNNNCTG 1 cut(s) 264
Ama87I CYCGRG 1 cut(s) 311
AoxI GGCC 3 cut(s) 307, 381, 691
ApeKI GCWGC 2 cut(s) 464, 485
Asp700I GAANNNNTTC 2 cut(s) 159, 611
AspLEI GCGC 2 cut(s) 624, 730
AspS9I GGNCC 1 cut(s) 449
AsuC2I CCSGG 1 cut(s) 133
AsuHPI GGTGA 2 cut(s) 188, 824
AvaI CYCGRG 1 cut(s) 311
AvaII GGWCC 1 cut(s) 449
BaeGI GKGCMC 1 cut(s) 772
BanI GGYRCC 1 cut(s) 548
BbsI GAAGAC 1 cut(s) 825
BbvI GCAGC 2 cut(s) 451, 497
BceAI ACGGC 1 cut(s) 193
BcnI CCSGG 1 cut(s) 133
BcoDI GTCTC 3 cut(s) 65, 218, 308
BfaI CTAG 2 cut(s) 515, 674
BfmI CTRYAG 1 cut(s) 869
BisI GCNGC 3 cut(s) 465, 486, 694
BlsI GCNGC 3 cut(s) 466, 487, 695
BmcAI AGTACT 1 cut(s) 855
Bme1390I CCNGG 1 cut(s) 133
Bme18I GGWCC 1 cut(s) 449
BmeT110I CYCGRG 1 cut(s) 311
BmgT120I GGNCC 1 cut(s) 449
BmiI GGNNCC 1 cut(s) 550
BmrFI CCNGG 1 cut(s) 133
BmsI GCATC 3 cut(s) 67, 517, 715
BpiI GAAGAC 1 cut(s) 825
BpuEI CTTGAG 1 cut(s) 317
BpuMI CCSGG 1 cut(s) 133
BsaI GGTCTC 1 cut(s) 65
BsaJI CCNNGG 3 cut(s) 247, 457, 713
BsaWI WCCGGW 1 cut(s) 232
BsaXI ACNNNNNCTCC 4 cut(s) 26, 56, 837, 867
Bsc4I CCNNNNNNNGG 1 cut(s) 458
Bse118I RCCGGY 1 cut(s) 305
Bse1I ACTGG 1 cut(s) 655
Bse3DI GCAATG 2 cut(s) 290, 700
BseDI CCNNGG 3 cut(s) 247, 457, 713
BseGI GGATG 1 cut(s) 730
BseLI CCNNNNNNNGG 1 cut(s) 458
BseMI GCAATG 2 cut(s) 290, 700
BseNI ACTGG 1 cut(s) 655
BseSI GKGCMC 1 cut(s) 772
BseXI GCAGC 2 cut(s) 451, 497
BshFI GGCC 3 cut(s) 309, 383, 693
BshNI GGYRCC 1 cut(s) 548
BsiHKCI CYCGRG 1 cut(s) 311
BsiSI CCGG 4 cut(s) 133, 233, 306, 591
BslI CCNNNNNNNGG 1 cut(s) 458
BsmAI GTCTC 3 cut(s) 65, 218, 308
BsmBI CGTCTC 1 cut(s) 218
BsmI GAATGC 1 cut(s) 80
BsnI GGCC 3 cut(s) 309, 383, 693
Bso31I GGTCTC 1 cut(s) 65
BsoBI CYCGRG 1 cut(s) 311
Bsp1286I GDGCHC 1 cut(s) 772
Bsp143I GATC 1 cut(s) 825
Bsp19I CCATGG 1 cut(s) 247
BspACI CCGC 2 cut(s) 39, 694
BspANI GGCC 3 cut(s) 309, 383, 693
BspHI TCATGA 1 cut(s) 741
BspLI GGNNCC 1 cut(s) 550
BspT107I GGYRCC 1 cut(s) 548
BspTNI GGTCTC 1 cut(s) 65
BsrDI GCAATG 2 cut(s) 290, 700
BsrFI RCCGGY 1 cut(s) 305
BsrI ACTGG 1 cut(s) 655
BssAI RCCGGY 1 cut(s) 305
BssECI CCNNGG 3 cut(s) 247, 457, 713
BssMI GATC 1 cut(s) 825
BssNAI GTATAC 1 cut(s) 540
BssT1I CCWWGG 2 cut(s) 247, 713
Bst1107I GTATAC 1 cut(s) 540
Bst4CI ACNGT 2 cut(s) 49, 67
Bst6I CTCTTC 1 cut(s) 602
BstC8I GCNNGC 4 cut(s) 19, 307, 490, 698
BstDSI CCRYGG 1 cut(s) 247
BstF5I GGATG 1 cut(s) 730
BstHHI GCGC 2 cut(s) 624, 730
BstKTI GATC 1 cut(s) 828
BstMAI GTCTC 3 cut(s) 65, 218, 308
BstMBI GATC 1 cut(s) 825
BstMWI GCNNNNNNNGC 2 cut(s) 470, 702
BstSCI CCNGG 1 cut(s) 131
BstSFI CTRYAG 1 cut(s) 869
BstSLI GKGCMC 1 cut(s) 772
BstV1I GCAGC 2 cut(s) 451, 497
BstV2I GAAGAC 1 cut(s) 825
BstZ17I GTATAC 1 cut(s) 540
BsuRI GGCC 3 cut(s) 309, 383, 693
BtgI CCRYGG 1 cut(s) 247
BtsCI GGATG 1 cut(s) 730
BtsIMutI CAGTG 2 cut(s) 63, 648
Cac8I GCNNGC 4 cut(s) 19, 307, 490, 698
CaiI CAGNNNCTG 1 cut(s) 264
CciI TCATGA 1 cut(s) 741
CfoI GCGC 2 cut(s) 624, 730
Cfr10I RCCGGY 1 cut(s) 305
Cfr13I GGNCC 1 cut(s) 449
Csp6I GTAC 2 cut(s) 666, 854
CviAII CATG 3 cut(s) 248, 372, 742
CviQI GTAC 2 cut(s) 666, 854
DpnI GATC 1 cut(s) 827
DpnII GATC 1 cut(s) 825
EaeI YGGCCR 1 cut(s) 691
Eam1104I CTCTTC 1 cut(s) 602
EarI CTCTTC 1 cut(s) 602
Eco130I CCWWGG 2 cut(s) 247, 713
Eco31I GGTCTC 1 cut(s) 65
Eco47I GGWCC 1 cut(s) 449
Eco88I CYCGRG 1 cut(s) 311
EcoT14I CCWWGG 2 cut(s) 247, 713
EcoT22I ATGCAT 1 cut(s) 82
ErhI CCWWGG 2 cut(s) 247, 713
Esp3I CGTCTC 1 cut(s) 218
FaeI CATG 3 cut(s) 251, 375, 745
FatI CATG 3 cut(s) 247, 371, 741
FauNDI CATATG 1 cut(s) 806
FblI GTMKAC 1 cut(s) 539
Fnu4HI GCNGC 3 cut(s) 465, 486, 694
FokI GGATG 1 cut(s) 737
Fsp4HI GCNGC 3 cut(s) 465, 486, 694
FspBI CTAG 2 cut(s) 515, 674
GlaI GCGC 2 cut(s) 623, 729
GluI GCNGC 3 cut(s) 465, 486, 694
HaeIII GGCC 3 cut(s) 309, 383, 693
HapII CCGG 4 cut(s) 133, 233, 306, 591
HhaI GCGC 2 cut(s) 624, 730
Hin1II CATG 3 cut(s) 251, 375, 745
Hin6I GCGC 2 cut(s) 622, 728
HinP1I GCGC 2 cut(s) 622, 728
HincII GTYRAC 2 cut(s) 6, 328
HindII GTYRAC 2 cut(s) 6, 328
HindIII AAGCTT 1 cut(s) 839
HinfI GANTC 3 cut(s) 150, 229, 363
HpaI GTTAAC 1 cut(s) 6
HpaII CCGG 4 cut(s) 133, 233, 306, 591
HphI GGTGA 2 cut(s) 188, 824
Hpy166II GTNNAC 4 cut(s) 6, 328, 540, 757
Hpy188I TCNGA 3 cut(s) 76, 219, 352
Hpy188III TCNNGA 4 cut(s) 109, 313, 497, 742
Hpy8I GTNNAC 4 cut(s) 6, 328, 540, 757
HpyAV CCTTC 3 cut(s) 96, 104, 539
HpyCH4III ACNGT 2 cut(s) 49, 67
HpyCH4V TGCA 5 cut(s) 80, 473, 485, 530, 700
HpyF10VI GCNNNNNNNGC 2 cut(s) 470, 702
Hsp92II CATG 3 cut(s) 251, 375, 745
HspAI GCGC 2 cut(s) 622, 728
KroI GCCGGC 1 cut(s) 305
KroNI GCCGGC 1 cut(s) 307
KspAI GTTAAC 1 cut(s) 6
Kzo9I GATC 1 cut(s) 825
LmnI GCTCC 4 cut(s) 77, 219, 266, 593
Lsp1109I GCAGC 2 cut(s) 451, 497
LweI GCATC 3 cut(s) 67, 517, 715
MaeI CTAG 2 cut(s) 515, 674
MaeIII GTNAC 2 cut(s) 392, 631
MalI GATC 1 cut(s) 827
MboI GATC 1 cut(s) 825
MboII GAAGA 3 cut(s) 233, 619, 830
MfeI CAATTG 1 cut(s) 474
MhlI GDGCHC 1 cut(s) 772
MluCI AATT 2 cut(s) 181, 474
MlyI GAGTC 2 cut(s) 223, 357
MmeI TCCRAC 1 cut(s) 427
Mph1103I ATGCAT 1 cut(s) 82
MroNI GCCGGC 1 cut(s) 305
MroXI GAANNNNTTC 2 cut(s) 159, 611
MseI TTAA 2 cut(s) 5, 426
MspI CCGG 4 cut(s) 133, 233, 306, 591
MspR9I CCNGG 1 cut(s) 133
MunI CAATTG 1 cut(s) 474
Mva1269I GAATGC 1 cut(s) 80
MwoI GCNNNNNNNGC 2 cut(s) 470, 702
NaeI GCCGGC 1 cut(s) 307
NciI CCSGG 1 cut(s) 133
NcoI CCATGG 1 cut(s) 247
NdeI CATATG 1 cut(s) 806
NdeII GATC 1 cut(s) 825
NgoMIV GCCGGC 1 cut(s) 305
NlaIII CATG 3 cut(s) 251, 375, 745
NlaIV GGNNCC 1 cut(s) 550
NmuCI GTSAC 1 cut(s) 392
NsiI ATGCAT 1 cut(s) 82
PaeR7I CTCGAG 1 cut(s) 311
PagI TCATGA 1 cut(s) 741
PctI GAATGC 1 cut(s) 80
PdiI GCCGGC 1 cut(s) 307
PdmI GAANNNNTTC 2 cut(s) 159, 611
PfeI GAWTC 1 cut(s) 150
PkrI GCNGC 3 cut(s) 466, 487, 695
PleI GAGTC 2 cut(s) 223, 357
PpsI GAGTC 2 cut(s) 223, 357
PspN4I GGNNCC 1 cut(s) 550
PspPI GGNCC 1 cut(s) 449
PstNI CAGNNNCTG 1 cut(s) 264
RsaI GTAC 2 cut(s) 667, 855
RsaNI GTAC 2 cut(s) 666, 854
SaqAI TTAA 2 cut(s) 5, 426
SatI GCNGC 3 cut(s) 465, 486, 694
Sau3AI GATC 1 cut(s) 825
Sau96I GGNCC 1 cut(s) 449
ScaI AGTACT 1 cut(s) 855
SchI GAGTC 2 cut(s) 223, 357
ScrFI CCNGG 1 cut(s) 133
SduI GDGCHC 1 cut(s) 772
SfaNI GCATC 3 cut(s) 67, 517, 715
SfcI CTRYAG 1 cut(s) 869
Sfr274I CTCGAG 1 cut(s) 311
SinI GGWCC 1 cut(s) 449
SlaI CTCGAG 1 cut(s) 311
SmlI CTYRAG 2 cut(s) 296, 311
SmoI CTYRAG 2 cut(s) 296, 311
Sse9I AATT 2 cut(s) 181, 474
SsiI CCGC 2 cut(s) 39, 694
SspI AATATT 1 cut(s) 688
SspMI CTAG 2 cut(s) 515, 674
StyD4I CCNGG 1 cut(s) 131
StyI CCWWGG 2 cut(s) 247, 713
TaaI ACNGT 2 cut(s) 49, 67
TaqI TCGA 4 cut(s) 108, 153, 312, 386
TasI AATT 2 cut(s) 181, 474
TatI WGTACW 1 cut(s) 853
TauI GCSGC 1 cut(s) 696
TfiI GAWTC 1 cut(s) 150
Tru1I TTAA 2 cut(s) 5, 426
Tru9I TTAA 2 cut(s) 5, 426
TscAI CASTG 2 cut(s) 70, 655
TseFI GTSAC 1 cut(s) 392
TseI GCWGC 2 cut(s) 464, 485
Tsp45I GTSAC 1 cut(s) 392
TspDTI ATGAA 1 cut(s) 730
TspRI CASTG 2 cut(s) 70, 655
VpaK11BI GGWCC 1 cut(s) 449
XhoI CTCGAG 1 cut(s) 311
XmiI GTMKAC 1 cut(s) 539
XmnI GAANNNNTTC 2 cut(s) 159, 611
XspI CTAG 2 cut(s) 515, 674
ZrmI AGTACT 1 cut(s) 855
Zsp2I ATGCAT 1 cut(s) 82
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.