FvH4_4g30600

eukaryotic translation initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0007738
Physical Location & Seq
Forward (+)
30128178 .. 30132291
4114 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g30600.t2

Sequence Viewer

Length: 960 bp
ATGAGCGGTGCCCCTTTGAAATTCGAATCCCCAATTCAAGACGCAATTTCCAGAATCCGATTCGCTCCAACCTCGCACAACCTCCTCATCTCTTCTTGGGACTCCAAACTGAGATTGTATGACGTGGAGAGCTCGCTGCTCAGATTAGAAGCTCCGTCGGAGGCTGCGCTACTCGATTGTTGCTTCCAGAATGAAACGGCGGCGTTTAGCGCCGGCTCTGATGGCTGTGTTAGGAGGTATGACTTGCATTCTGGAAGTTGTGAGACGATTGGGAACCATGATGACATAGCAACCTGCATTGGATATTCCGATGAAACAAGCCAGGTAATCAGTGCTGGTTTGGACAAGAAGATATATGCCTGGGACGCACGAATGGAGAAGGGTAAAGGATATGCGTTTGAAAGTCTCAACTCAGAGGTGACATCCATGTCACTCTCGGGTCTCAACATGATGGTAACCATGGGAGCATCAATACTCATGTGTGATTTGCGAAGCCCTCTAAAACCATTTGAATCAAAAGATTCAGATTTGAAGACAACGATTATATGTGGTAGTGCGAGTCCGCATGCTAAAGGATTTGCTGTTGGCTCTGCAGATGGACGAGTATCCTTGGAGATTCTCCATCCATCTAATTCAGATACTATCAGATATATGTTCCGGTGTCATCCGAAGTCAACTGATAGAAGATATCATCTAGCAGCGGTGAATGAAATTGTGTTCAATCCTATCATTTACGGTGCCCTTCTTACCGGAGATGATGAGGGTCATGTTATTGCATGGGATGCTCAAACCAGAAAGAGGCTGTTTCAGTTGCCTAAGTACCCAAATAGTGTGGCTTCCTTATCGTATCACCACCAGGGACGACTGTTGGCTGTTGCATCAAGCTACACATATCGGCAAGCTATTGAATTAGAAGAGGCCCCTCAGATATTTATACATGATTCTGGCGATGCTAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko05166 map04110 map04111 map05166
Pfam Domains
Protein Families
eIF-5a

Protein Analysis

320

Amino Acids

35.06

Weight (kDa)

5.65

Isoelectric Point (pI)

44.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR5 PF25175 13 - 146 9.1e-13 WDR5 beta-propeller domain
WD40_CDC20-Fz PF24807 13 - 144 1.8e-06 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 14 - 122 5.7e-08 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 14 - 204 2.9e-11 THOC3 beta-propeller domain
WD40_Prp19 PF24814 58 - 295 2.3e-12 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 66 - 146 3.7e-07 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 91 - 298 8e-09 CDC20/Fizzy WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 427
Acc36I ACCTGC 1 cut(s) 302
AccB1I GGYRCC 2 cut(s) 8, 737
AccBSI CCGCTC 1 cut(s) 6
AciI CCGC 4 cut(s) 6, 200, 563, 701
AcsI RAATTY 1 cut(s) 20
AfaI GTAC 1 cut(s) 821
AfiI CCNNNNNNNGG 1 cut(s) 798
AgsI TTSAA 7 cut(s) 19, 38, 401, 512, 532, 721, 908
AjiI CACGTC 1 cut(s) 124
AjnI CCWGG 3 cut(s) 321, 359, 855
AluBI AGCT 4 cut(s) 132, 152, 885, 902
AluI AGCT 4 cut(s) 132, 152, 885, 902
Alw21I GWGCWC 1 cut(s) 134
Alw26I GTCTC 3 cut(s) 257, 410, 446
Ama87I CYCGRG 1 cut(s) 436
AoxI GGCC 1 cut(s) 918
ApeKI GCWGC 3 cut(s) 136, 164, 698
ApoI RAATTY 1 cut(s) 20
AspLEI GCGC 2 cut(s) 169, 212
AspS9I GGNCC 1 cut(s) 919
AsuHPI GGTGA 3 cut(s) 430, 715, 842
AsuII TTCGAA 1 cut(s) 24
AvaI CYCGRG 1 cut(s) 436
BaeGI GKGCMC 2 cut(s) 13, 742
BanI GGYRCC 2 cut(s) 8, 737
BanII GRGCYC 1 cut(s) 134
BbsI GAAGAC 1 cut(s) 539
Bbv12I GWGCWC 1 cut(s) 134
BbvI GCAGC 3 cut(s) 123, 151, 710
BccI CCATC 5 cut(s) 215, 445, 590, 630, 634
BceAI ACGGC 1 cut(s) 213
BciT130I CCWGG 3 cut(s) 323, 361, 857
BciVI GTATCC 1 cut(s) 616
BcoDI GTCTC 3 cut(s) 257, 410, 446
BfaI CTAG 1 cut(s) 695
BfmI CTRYAG 1 cut(s) 591
BfoI RGCGCY 1 cut(s) 213
BfuAI ACCTGC 1 cut(s) 302
BfuI GTATCC 1 cut(s) 616
BisI GCNGC 4 cut(s) 137, 165, 201, 699
BlsI GCNGC 4 cut(s) 138, 166, 202, 700
Bme1390I CCNGG 3 cut(s) 323, 361, 857
BmeT110I CYCGRG 1 cut(s) 436
BmgBI CACGTC 1 cut(s) 124
BmgT120I GGNCC 1 cut(s) 919
BmiI GGNNCC 4 cut(s) 10, 275, 739, 921
BmrFI CCNGG 3 cut(s) 323, 361, 857
BmsI GCATC 4 cut(s) 476, 772, 887, 940
BpiI GAAGAC 1 cut(s) 539
Bpu14I TTCGAA 1 cut(s) 24
BsaI GGTCTC 1 cut(s) 446
BsaJI CCNNGG 4 cut(s) 360, 459, 609, 856
BsaWI WCCGGW 2 cut(s) 657, 749
Bsc4I CCNNNNNNNGG 1 cut(s) 798
Bse118I RCCGGY 1 cut(s) 212
BseBI CCWGG 3 cut(s) 323, 361, 857
BseDI CCNNGG 4 cut(s) 360, 459, 609, 856
BseGI GGATG 4 cut(s) 422, 622, 664, 787
BseLI CCNNNNNNNGG 1 cut(s) 798
BseMII CTCAG 4 cut(s) 101, 154, 426, 938
BseRI GAGGAG 1 cut(s) 74
BseSI GKGCMC 2 cut(s) 13, 742
BseXI GCAGC 3 cut(s) 123, 151, 710
BshFI GGCC 1 cut(s) 920
BshNI GGYRCC 2 cut(s) 8, 737
BsiHKAI GWGCWC 1 cut(s) 134
BsiHKCI CYCGRG 1 cut(s) 436
BsiSI CCGG 3 cut(s) 213, 658, 750
BslFI GGGAC 3 cut(s) 113, 377, 873
BslI CCNNNNNNNGG 1 cut(s) 798
BsmAI GTCTC 3 cut(s) 257, 410, 446
BsmBI CGTCTC 1 cut(s) 257
BsmFI GGGAC 3 cut(s) 113, 377, 873
BsmI GAATGC 1 cut(s) 247
BsnI GGCC 1 cut(s) 920
Bso31I GGTCTC 1 cut(s) 446
BsoBI CYCGRG 1 cut(s) 436
Bsp119I TTCGAA 1 cut(s) 24
Bsp1286I GDGCHC 3 cut(s) 13, 134, 742
Bsp19I CCATGG 1 cut(s) 459
BspACI CCGC 4 cut(s) 6, 200, 563, 701
BspANI GGCC 1 cut(s) 920
BspCNI CTCAG 4 cut(s) 102, 153, 425, 937
BspLI GGNNCC 4 cut(s) 10, 275, 739, 921
BspMAI CTGCAG 1 cut(s) 595
BspMI ACCTGC 1 cut(s) 302
BspT104I TTCGAA 1 cut(s) 24
BspT107I GGYRCC 2 cut(s) 8, 737
BspTNI GGTCTC 1 cut(s) 446
BsrBI CCGCTC 1 cut(s) 6
BsrFI RCCGGY 1 cut(s) 212
BssAI RCCGGY 1 cut(s) 212
BssECI CCNNGG 4 cut(s) 360, 459, 609, 856
BssT1I CCWWGG 2 cut(s) 459, 609
Bst2UI CCWGG 3 cut(s) 323, 361, 857
Bst4CI ACNGT 2 cut(s) 737, 867
Bst6I CTCTTC 2 cut(s) 97, 909
BstAPI GCANNNNNTGC 1 cut(s) 782
BstBI TTCGAA 1 cut(s) 24
BstC8I GCNNGC 4 cut(s) 134, 214, 567, 900
BstDEI CTNAG 5 cut(s) 110, 140, 412, 816, 924
BstDSI CCRYGG 1 cut(s) 459
BstEII GGTNACC 1 cut(s) 454
BstF5I GGATG 4 cut(s) 422, 622, 664, 787
BstH2I RGCGCY 1 cut(s) 213
BstHHI GCGC 2 cut(s) 169, 212
BstMAI GTCTC 3 cut(s) 257, 410, 446
BstMWI GCNNNNNNNGC 4 cut(s) 209, 222, 365, 782
BstNI CCWGG 3 cut(s) 323, 361, 857
BstNSI RCATGY 1 cut(s) 569
BstPI GGTNACC 1 cut(s) 454
BstSCI CCNGG 3 cut(s) 321, 359, 855
BstSFI CTRYAG 1 cut(s) 591
BstSLI GKGCMC 2 cut(s) 13, 742
BstV1I GCAGC 3 cut(s) 123, 151, 710
BstV2I GAAGAC 1 cut(s) 539
BsuI GTATCC 1 cut(s) 616
BsuRI GGCC 1 cut(s) 920
BtgI CCRYGG 1 cut(s) 459
BtrI CACGTC 1 cut(s) 124
BtsCI GGATG 4 cut(s) 422, 622, 664, 787
BtsIMutI CAGTG 1 cut(s) 337
BveI ACCTGC 1 cut(s) 302
Cac8I GCNNGC 4 cut(s) 134, 214, 567, 900
CfoI GCGC 2 cut(s) 169, 212
Cfr10I RCCGGY 1 cut(s) 212
Cfr13I GGNCC 1 cut(s) 919
CseI GACGC 2 cut(s) 50, 374
Csp6I GTAC 1 cut(s) 820
CspCI CAANNNNNGTGG 2 cut(s) 813, 848
CviAII CATG 9 cut(s) 278, 427, 448, 460, 478, 566, 767, 777, 938
CviQI GTAC 1 cut(s) 820
DdeI CTNAG 5 cut(s) 110, 140, 412, 816, 924
DrdI GACNNNNNNGTC 1 cut(s) 427
DseDI GACNNNNNNGTC 1 cut(s) 427
Eam1104I CTCTTC 2 cut(s) 97, 909
EarI CTCTTC 2 cut(s) 97, 909
Ecl136II GAGCTC 1 cut(s) 132
Eco130I CCWWGG 2 cut(s) 459, 609
Eco24I GRGCYC 1 cut(s) 134
Eco31I GGTCTC 1 cut(s) 446
Eco32I GATATC 1 cut(s) 689
Eco53kI GAGCTC 1 cut(s) 132
Eco88I CYCGRG 1 cut(s) 436
Eco91I GGTNACC 1 cut(s) 454
EcoICRI GAGCTC 1 cut(s) 132
EcoO109I RGGNCCY 1 cut(s) 919
EcoO65I GGTNACC 1 cut(s) 454
EcoRII CCWGG 3 cut(s) 321, 359, 855
EcoRV GATATC 1 cut(s) 689
EcoT14I CCWWGG 2 cut(s) 459, 609
EcoT38I GRGCYC 1 cut(s) 134
ErhI CCWWGG 2 cut(s) 459, 609
Esp3I CGTCTC 1 cut(s) 257
FaeI CATG 9 cut(s) 281, 430, 451, 463, 481, 569, 770, 780, 941
FaqI GGGAC 3 cut(s) 113, 377, 873
FatI CATG 9 cut(s) 277, 426, 447, 459, 477, 565, 766, 776, 937
Fnu4HI GCNGC 4 cut(s) 137, 165, 201, 699
FokI GGATG 4 cut(s) 409, 609, 651, 794
FriOI GRGCYC 1 cut(s) 134
Fsp4HI GCNGC 4 cut(s) 137, 165, 201, 699
FspBI CTAG 1 cut(s) 695
GlaI GCGC 2 cut(s) 168, 211
GluI GCNGC 4 cut(s) 137, 165, 201, 699
HaeII RGCGCY 1 cut(s) 213
HaeIII GGCC 1 cut(s) 920
HapII CCGG 3 cut(s) 213, 658, 750
HgaI GACGC 2 cut(s) 50, 374
HhaI GCGC 2 cut(s) 169, 212
Hin1II CATG 9 cut(s) 281, 430, 451, 463, 481, 569, 770, 780, 941
Hin6I GCGC 2 cut(s) 167, 210
HinP1I GCGC 2 cut(s) 167, 210
HincII GTYRAC 1 cut(s) 675
HindII GTYRAC 1 cut(s) 675
HinfI GANTC 9 cut(s) 26, 54, 60, 101, 512, 521, 559, 616, 941
HpaII CCGG 3 cut(s) 213, 658, 750
HphI GGTGA 3 cut(s) 430, 715, 842
Hpy166II GTNNAC 1 cut(s) 675
Hpy188III TCNNGA 4 cut(s) 38, 51, 187, 252
Hpy8I GTNNAC 1 cut(s) 675
Hpy99I CGWCG 1 cut(s) 160
HpyAV CCTTC 2 cut(s) 373, 752
HpyCH4III ACNGT 2 cut(s) 737, 867
HpyCH4IV ACGT 1 cut(s) 123
HpyCH4V TGCA 5 cut(s) 247, 297, 593, 776, 878
HpyF10VI GCNNNNNNNGC 4 cut(s) 209, 222, 365, 782
HpyF3I CTNAG 5 cut(s) 110, 140, 412, 816, 924
HpySE526I ACGT 1 cut(s) 123
Hsp92II CATG 9 cut(s) 281, 430, 451, 463, 481, 569, 770, 780, 941
HspAI GCGC 2 cut(s) 167, 210
KroI GCCGGC 1 cut(s) 212
KroNI GCCGGC 1 cut(s) 214
LmnI GCTCC 3 cut(s) 70, 157, 464
Lsp1109I GCAGC 3 cut(s) 123, 151, 710
LweI GCATC 4 cut(s) 476, 772, 887, 940
MaeI CTAG 1 cut(s) 695
MaeII ACGT 1 cut(s) 123
MaeIII GTNAC 3 cut(s) 418, 429, 454
MbiI CCGCTC 1 cut(s) 6
MboII GAAGA 5 cut(s) 84, 361, 544, 696, 926
MhlI GDGCHC 3 cut(s) 13, 134, 742
MluCI AATT 6 cut(s) 20, 33, 45, 631, 711, 908
MlyI GAGTC 2 cut(s) 95, 568
MmeI TCCRAC 2 cut(s) 92, 138
MroNI GCCGGC 1 cut(s) 212
MspA1I CMGCKG 1 cut(s) 701
MspI CCGG 3 cut(s) 213, 658, 750
MspR9I CCNGG 3 cut(s) 323, 361, 857
Mva1269I GAATGC 1 cut(s) 247
MvaI CCWGG 3 cut(s) 323, 361, 857
MwoI GCNNNNNNNGC 4 cut(s) 209, 222, 365, 782
NaeI GCCGGC 1 cut(s) 214
NcoI CCATGG 1 cut(s) 459
NgoMIV GCCGGC 1 cut(s) 212
NlaIII CATG 9 cut(s) 281, 430, 451, 463, 481, 569, 770, 780, 941
NlaIV GGNNCC 4 cut(s) 10, 275, 739, 921
NmuCI GTSAC 2 cut(s) 418, 429
NspI RCATGY 1 cut(s) 569
NspV TTCGAA 1 cut(s) 24
PaeI GCATGC 1 cut(s) 569
PctI GAATGC 1 cut(s) 247
PdiI GCCGGC 1 cut(s) 214
PfeI GAWTC 7 cut(s) 26, 54, 60, 512, 521, 616, 941
PkrI GCNGC 4 cut(s) 138, 166, 202, 700
PleI GAGTC 2 cut(s) 95, 567
PpsI GAGTC 2 cut(s) 95, 567
Psp124BI GAGCTC 1 cut(s) 134
Psp6I CCWGG 3 cut(s) 321, 359, 855
PspEI GGTNACC 1 cut(s) 454
PspGI CCWGG 3 cut(s) 321, 359, 855
PspN4I GGNNCC 4 cut(s) 10, 275, 739, 921
PspPI GGNCC 1 cut(s) 919
PstI CTGCAG 1 cut(s) 595
RsaI GTAC 1 cut(s) 821
RsaNI GTAC 1 cut(s) 820
SacI GAGCTC 1 cut(s) 134
SatI GCNGC 4 cut(s) 137, 165, 201, 699
Sau96I GGNCC 1 cut(s) 919
SchI GAGTC 2 cut(s) 95, 568
ScrFI CCNGG 3 cut(s) 323, 361, 857
SduI GDGCHC 3 cut(s) 13, 134, 742
SfaNI GCATC 4 cut(s) 476, 772, 887, 940
SfcI CTRYAG 1 cut(s) 591
SfuI TTCGAA 1 cut(s) 24
SphI GCATGC 1 cut(s) 569
Sse9I AATT 6 cut(s) 20, 33, 45, 631, 711, 908
SsiI CCGC 4 cut(s) 6, 200, 563, 701
SspMI CTAG 1 cut(s) 695
SstI GAGCTC 1 cut(s) 134
StyD4I CCNGG 3 cut(s) 321, 359, 855
StyI CCWWGG 2 cut(s) 459, 609
TaaI ACNGT 2 cut(s) 737, 867
TaiI ACGT 1 cut(s) 126
TaqI TCGA 2 cut(s) 24, 174
TasI AATT 6 cut(s) 20, 33, 45, 631, 711, 908
TauI GCSGC 1 cut(s) 203
TfiI GAWTC 7 cut(s) 26, 54, 60, 512, 521, 616, 941
TscAI CASTG 1 cut(s) 337
TseFI GTSAC 2 cut(s) 418, 429
TseI GCWGC 3 cut(s) 136, 164, 698
Tsp45I GTSAC 2 cut(s) 418, 429
TspDTI ATGAA 3 cut(s) 207, 327, 723
TspGWI ACGGA 1 cut(s) 144
TspRI CASTG 1 cut(s) 337
XapI RAATTY 1 cut(s) 20
XceI RCATGY 1 cut(s) 569
XspI CTAG 1 cut(s) 695
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.