Rw0G007390

eukaryotic translation initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00270
OG0007738
Physical Location & Seq
Forward (+)
196576 .. 199907
3332 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G007390.1

Sequence Viewer

Length: 1107 bp
ATGAGCGGAGCCCCTTTGAAATTCGAAACCCCAATCCAAGATGCAATTTCCAGAATCCGATTCGCTCCAACCTCACACAACCTTCTCATCGCTTCTTGGGACTCCAAACTGCGACTGTACGACGTGGGAAGCTCGGTGCTCAGATTCGATGCTCCGGCCGAAGCTGCACTACTCGATTGTTGCTTCCAGAACGAGTCGGTTGCGTTTAGCACCGGCTCCGATGGCTTCATTAGAAGGTATGACTTGCATTCTGGAATCTGTGAGATGATTGGAAATCATGATGACATAGCAACCTGCATTGGATATTCTGATGAAACAAGCCAAGTACTTACCGCTGGTTTGGACAAGAAGATATTTGCCTGGGACGCACGTATGGAGGAGGGTAAAAAGTCTGTGTTTGTAAAAAGTCTTGACTCAGAGGTGACGTCCATGTCACTCTCGGGTCTCGATATGATGGTAACCATGGGAGCGTTAATATATGTGTGTGATTTGCGCAACCTCCAAAAACCATATGAATCAAAAGTTTCAGATTTGAACACAACGATTATATGTGGAAGTGCGATTCCTAATGCTAAAGGATTTGCGGTTGGCTCAGCAGATGGACGAGTGTCATTGGAGATTTGCCATCCATCTAATTCAGATAACATCAGATATATGTTCCGGTGTCATCCGAAGTCAACTGATAGAAGATATCATCTAGCAGCGGTGAATGAAATTGTGTTCAATCCTCTCATTTACGGTGCCCTTCTTACTGGTGATGATGAGGGTCATGTTATTGCATGGAATGCTCAAAGCAGAAAGAGGCTATTTCAGTTGCCTAGGTACCCAAATAGCGTGGCATCCTTAGCATATCACCACCAGGGACAACTTCTGGCCGTTGCATCAAGCTACACATATCGGCAAGCTATTGAATCGTTAGTAATTTTCGAATCGTTAGTAATTTTCTACTGTCATCTACTTTTATCTGCACCATTACTGGATATGTTGTTTTATTCTCCTTCATGTTCACTTCCTTGTGACAGAGAAGAGCTCCCTCAGATATTTATACATGAAATTGGCGATGCTAACATGAGATCAGCTGACATAGGAAGTTCGAGCTGCAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko05166 map04110 map04111 map05166
Pfam Domains
Protein Families
eIF-5a

Protein Analysis

368

Amino Acids

40.69

Weight (kDa)

5.45

Isoelectric Point (pI)

45.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR5 PF25175 13 - 295 4e-16 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 14 - 122 2.6e-06 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 14 - 205 5.2e-07 THOC3 beta-propeller domain
WD40_Prp19 PF24814 58 - 296 7.4e-10 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 92 - 298 1.6e-07 CDC20/Fizzy WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 430
AatII GACGTC 1 cut(s) 428
Acc16I TGCGCA 1 cut(s) 494
Acc36I ACCTGC 1 cut(s) 302
Acc65I GGTACC 1 cut(s) 822
AccB1I GGYRCC 2 cut(s) 740, 822
AccBSI CCGCTC 1 cut(s) 6
AciI CCGC 4 cut(s) 6, 333, 584, 704
AcoI YGGCCR 2 cut(s) 156, 873
AcsI RAATTY 1 cut(s) 20
AcyI GRCGYC 1 cut(s) 425
AfaI GTAC 3 cut(s) 119, 327, 824
AgsI TTSAA 4 cut(s) 19, 535, 724, 911
AjiI CACGTC 1 cut(s) 124
AjnI CCWGG 2 cut(s) 359, 858
AluBI AGCT 7 cut(s) 132, 164, 888, 905, 1030, 1079, 1098
AluI AGCT 7 cut(s) 132, 164, 888, 905, 1030, 1079, 1098
Alw21I GWGCWC 2 cut(s) 141, 1032
Alw26I GTCTC 1 cut(s) 449
Ama87I CYCGRG 1 cut(s) 439
AoxI GGCC 2 cut(s) 156, 873
ApeKI GCWGC 3 cut(s) 164, 701, 1098
ApoI RAATTY 1 cut(s) 20
Asp718I GGTACC 1 cut(s) 822
AspA2I CCTAGG 1 cut(s) 818
AspLEI GCGC 1 cut(s) 495
AsuHPI GGTGA 4 cut(s) 433, 718, 767, 845
AsuII TTCGAA 2 cut(s) 24, 927
AvaI CYCGRG 1 cut(s) 439
AvrII CCTAGG 1 cut(s) 818
BaeGI GKGCMC 1 cut(s) 745
BanI GGYRCC 2 cut(s) 740, 822
BanII GRGCYC 2 cut(s) 13, 1032
BarI GAAGNNNNNNTAC 2 cut(s) 153, 185
Bbv12I GWGCWC 2 cut(s) 141, 1032
BbvI GCAGC 3 cut(s) 151, 713, 1085
BccI CCATC 5 cut(s) 215, 448, 593, 633, 637
BceAI ACGGC 1 cut(s) 860
BcgI CGANNNNNNTGC 2 cut(s) 182, 216
BciT130I CCWGG 2 cut(s) 361, 860
BcoDI GTCTC 1 cut(s) 449
BfaI CTAG 2 cut(s) 698, 819
BfuAI ACCTGC 1 cut(s) 302
BisI GCNGC 3 cut(s) 165, 702, 1099
BlnI CCTAGG 1 cut(s) 818
BlpI GCTNAGC 1 cut(s) 592
BlsI GCNGC 3 cut(s) 166, 703, 1100
BmcAI AGTACT 1 cut(s) 327
Bme1390I CCNGG 2 cut(s) 361, 860
BmeT110I CYCGRG 1 cut(s) 439
BmgBI CACGTC 1 cut(s) 124
BmiI GGNNCC 4 cut(s) 10, 217, 742, 824
BmrFI CCNGG 2 cut(s) 361, 860
BmsI GCATC 5 cut(s) 31, 139, 848, 890, 1051
BoxI GACNNNNGTC 1 cut(s) 607
BplI GAGNNNNNCTC 2 cut(s) 1014, 1046
Bpu10I CCTNAGC 1 cut(s) 844
Bpu1102I GCTNAGC 1 cut(s) 592
Bpu14I TTCGAA 2 cut(s) 24, 927
BsaAI YACGTR 1 cut(s) 371
BsaHI GRCGYC 1 cut(s) 425
BsaI GGTCTC 1 cut(s) 449
BsaJI CCNNGG 4 cut(s) 360, 462, 818, 859
BsaWI WCCGGW 1 cut(s) 660
Bse118I RCCGGY 1 cut(s) 212
Bse1I ACTGG 2 cut(s) 757, 981
BseBI CCWGG 2 cut(s) 361, 860
BseDI CCNNGG 4 cut(s) 360, 462, 818, 859
BseGI GGATG 3 cut(s) 625, 667, 839
BseMII CTCAG 4 cut(s) 154, 429, 606, 1049
BseNI ACTGG 2 cut(s) 757, 981
BseRI GAGGAG 1 cut(s) 392
BseSI GKGCMC 1 cut(s) 745
BseX3I CGGCCG 1 cut(s) 156
BseXI GCAGC 3 cut(s) 151, 713, 1085
BsgI GTGCAG 2 cut(s) 150, 951
Bsh1285I CGRYCG 1 cut(s) 159
BshFI GGCC 2 cut(s) 158, 875
BshNI GGYRCC 2 cut(s) 740, 822
BsiEI CGRYCG 1 cut(s) 159
BsiHKAI GWGCWC 2 cut(s) 141, 1032
BsiHKCI CYCGRG 1 cut(s) 439
BsiSI CCGG 3 cut(s) 155, 213, 661
BslFI GGGAC 3 cut(s) 113, 377, 876
BsmAI GTCTC 1 cut(s) 449
BsmFI GGGAC 3 cut(s) 113, 377, 876
BsmI GAATGC 2 cut(s) 247, 790
BsnI GGCC 2 cut(s) 158, 875
Bso31I GGTCTC 1 cut(s) 449
BsoBI CYCGRG 1 cut(s) 439
Bsp119I TTCGAA 2 cut(s) 24, 927
Bsp1286I GDGCHC 4 cut(s) 13, 141, 745, 1032
Bsp143I GATC 1 cut(s) 1073
Bsp1720I GCTNAGC 1 cut(s) 592
Bsp19I CCATGG 1 cut(s) 462
BspACI CCGC 4 cut(s) 6, 333, 584, 704
BspANI GGCC 2 cut(s) 158, 875
BspCNI CTCAG 4 cut(s) 153, 428, 605, 1048
BspHI TCATGA 1 cut(s) 277
BspLI GGNNCC 4 cut(s) 10, 217, 742, 824
BspMI ACCTGC 1 cut(s) 302
BspQI GCTCTTC 1 cut(s) 1020
BspT104I TTCGAA 2 cut(s) 24, 927
BspT107I GGYRCC 2 cut(s) 740, 822
BspTNI GGTCTC 1 cut(s) 449
BsrBI CCGCTC 1 cut(s) 6
BsrFI RCCGGY 1 cut(s) 212
BsrI ACTGG 2 cut(s) 757, 981
BssAI RCCGGY 1 cut(s) 212
BssECI CCNNGG 4 cut(s) 360, 462, 818, 859
BssMI GATC 1 cut(s) 1073
BssNI GRCGYC 1 cut(s) 425
BssT1I CCWWGG 2 cut(s) 462, 818
Bst2UI CCWGG 2 cut(s) 361, 860
Bst4CI ACNGT 3 cut(s) 117, 740, 950
Bst6I CTCTTC 1 cut(s) 1020
BstACI GRCGYC 1 cut(s) 425
BstAPI GCANNNNNTGC 1 cut(s) 785
BstBAI YACGTR 1 cut(s) 371
BstBI TTCGAA 2 cut(s) 24, 927
BstC8I GCNNGC 1 cut(s) 903
BstDEI CTNAG 5 cut(s) 140, 415, 592, 844, 1035
BstDSI CCRYGG 1 cut(s) 462
BstEII GGTNACC 1 cut(s) 457
BstF5I GGATG 3 cut(s) 625, 667, 839
BstHHI GCGC 1 cut(s) 495
BstKTI GATC 1 cut(s) 1076
BstMAI GTCTC 1 cut(s) 449
BstMBI GATC 1 cut(s) 1073
BstMCI CGRYCG 1 cut(s) 159
BstMWI GCNNNNNNNGC 5 cut(s) 164, 222, 365, 785, 845
BstNI CCWGG 2 cut(s) 361, 860
BstPAI GACNNNNGTC 1 cut(s) 607
BstPI GGTNACC 1 cut(s) 457
BstSCI CCNGG 2 cut(s) 359, 858
BstSLI GKGCMC 1 cut(s) 745
BstV1I GCAGC 3 cut(s) 151, 713, 1085
BstZI CGGCCG 1 cut(s) 156
BsuRI GGCC 2 cut(s) 158, 875
BtgI CCRYGG 1 cut(s) 462
BtgZI GCGATG 2 cut(s) 73, 1074
BtrI CACGTC 1 cut(s) 124
BtsCI GGATG 3 cut(s) 625, 667, 839
BveI ACCTGC 1 cut(s) 302
Cac8I GCNNGC 1 cut(s) 903
CciI TCATGA 1 cut(s) 277
CfoI GCGC 1 cut(s) 495
Cfr10I RCCGGY 1 cut(s) 212
CseI GACGC 1 cut(s) 374
Csp6I GTAC 3 cut(s) 118, 326, 823
CspCI CAANNNNNGTGG 2 cut(s) 816, 851
CviAII CATG 8 cut(s) 278, 430, 463, 770, 780, 1002, 1049, 1069
CviQI GTAC 3 cut(s) 118, 326, 823
DdeI CTNAG 5 cut(s) 140, 415, 592, 844, 1035
DpnI GATC 1 cut(s) 1075
DpnII GATC 1 cut(s) 1073
DrdI GACNNNNNNGTC 1 cut(s) 430
DseDI GACNNNNNNGTC 1 cut(s) 430
EaeI YGGCCR 2 cut(s) 156, 873
EagI CGGCCG 1 cut(s) 156
Eam1104I CTCTTC 1 cut(s) 1020
EarI CTCTTC 1 cut(s) 1020
Ecl136II GAGCTC 1 cut(s) 1030
EclXI CGGCCG 1 cut(s) 156
Eco130I CCWWGG 2 cut(s) 462, 818
Eco24I GRGCYC 2 cut(s) 13, 1032
Eco31I GGTCTC 1 cut(s) 449
Eco32I GATATC 1 cut(s) 692
Eco52I CGGCCG 1 cut(s) 156
Eco53kI GAGCTC 1 cut(s) 1030
Eco88I CYCGRG 1 cut(s) 439
Eco91I GGTNACC 1 cut(s) 457
EcoICRI GAGCTC 1 cut(s) 1030
EcoO65I GGTNACC 1 cut(s) 457
EcoRII CCWGG 2 cut(s) 359, 858
EcoRV GATATC 1 cut(s) 692
EcoT14I CCWWGG 2 cut(s) 462, 818
EcoT38I GRGCYC 2 cut(s) 13, 1032
ErhI CCWWGG 2 cut(s) 462, 818
FaeI CATG 8 cut(s) 281, 433, 466, 773, 783, 1005, 1052, 1072
FaqI GGGAC 3 cut(s) 113, 377, 876
FatI CATG 8 cut(s) 277, 429, 462, 769, 779, 1001, 1048, 1068
FauNDI CATATG 1 cut(s) 511
Fnu4HI GCNGC 3 cut(s) 165, 702, 1099
FokI GGATG 3 cut(s) 612, 654, 826
FriOI GRGCYC 2 cut(s) 13, 1032
Fsp4HI GCNGC 3 cut(s) 165, 702, 1099
FspBI CTAG 2 cut(s) 698, 819
FspI TGCGCA 1 cut(s) 494
GlaI GCGC 1 cut(s) 494
GluI GCNGC 3 cut(s) 165, 702, 1099
HaeIII GGCC 2 cut(s) 158, 875
HapII CCGG 3 cut(s) 155, 213, 661
HgaI GACGC 1 cut(s) 374
HhaI GCGC 1 cut(s) 495
Hin1I GRCGYC 1 cut(s) 425
Hin1II CATG 8 cut(s) 281, 433, 466, 773, 783, 1005, 1052, 1072
Hin6I GCGC 1 cut(s) 493
HinP1I GCGC 1 cut(s) 493
HincII GTYRAC 1 cut(s) 678
HindII GTYRAC 1 cut(s) 678
HpaII CCGG 3 cut(s) 155, 213, 661
HphI GGTGA 4 cut(s) 433, 718, 767, 845
Hpy166II GTNNAC 2 cut(s) 678, 1007
Hpy188III TCNNGA 6 cut(s) 51, 187, 252, 278, 410, 446
Hpy8I GTNNAC 2 cut(s) 678, 1007
Hpy99I CGWCG 1 cut(s) 125
HpyAV CCTTC 4 cut(s) 92, 228, 755, 1008
HpyCH4III ACNGT 3 cut(s) 117, 740, 950
HpyCH4IV ACGT 3 cut(s) 123, 370, 425
HpyCH4V TGCA 8 cut(s) 44, 167, 247, 297, 779, 881, 968, 1101
HpyF10VI GCNNNNNNNGC 5 cut(s) 164, 222, 365, 785, 845
HpyF3I CTNAG 5 cut(s) 140, 415, 592, 844, 1035
HpySE526I ACGT 3 cut(s) 123, 370, 425
Hsp92I GRCGYC 1 cut(s) 425
Hsp92II CATG 8 cut(s) 281, 433, 466, 773, 783, 1005, 1052, 1072
HspAI GCGC 1 cut(s) 493
KpnI GGTACC 1 cut(s) 826
Kzo9I GATC 1 cut(s) 1073
LguI GCTCTTC 1 cut(s) 1020
LmnI GCTCC 6 cut(s) 8, 70, 157, 221, 467, 1035
Lsp1109I GCAGC 3 cut(s) 151, 713, 1085
LweI GCATC 5 cut(s) 31, 139, 848, 890, 1051
MaeI CTAG 2 cut(s) 698, 819
MaeII ACGT 3 cut(s) 123, 370, 425
MaeIII GTNAC 4 cut(s) 421, 432, 457, 1016
MalI GATC 1 cut(s) 1075
MbiI CCGCTC 1 cut(s) 6
MboI GATC 1 cut(s) 1073
MboII GAAGA 3 cut(s) 361, 699, 1037
MhlI GDGCHC 4 cut(s) 13, 141, 745, 1032
MluCI AATT 7 cut(s) 20, 45, 634, 714, 921, 939, 1053
MlyI GAGTC 3 cut(s) 95, 203, 407
MmeI TCCRAC 1 cut(s) 92
MnlI CCTC 9 cut(s) 82, 370, 373, 412, 509, 738, 757, 795, 1044
MseI TTAA 1 cut(s) 473
MspA1I CMGCKG 3 cut(s) 335, 704, 1079
MspI CCGG 3 cut(s) 155, 213, 661
MspR9I CCNGG 2 cut(s) 361, 860
Mva1269I GAATGC 2 cut(s) 247, 790
MvaI CCWGG 2 cut(s) 361, 860
MwoI GCNNNNNNNGC 5 cut(s) 164, 222, 365, 785, 845
NcoI CCATGG 1 cut(s) 462
NdeI CATATG 1 cut(s) 511
NdeII GATC 1 cut(s) 1073
NlaIII CATG 8 cut(s) 281, 433, 466, 773, 783, 1005, 1052, 1072
NlaIV GGNNCC 4 cut(s) 10, 217, 742, 824
NmuCI GTSAC 3 cut(s) 421, 432, 1016
NsbI TGCGCA 1 cut(s) 494
NspV TTCGAA 2 cut(s) 24, 927
PagI TCATGA 1 cut(s) 277
PciSI GCTCTTC 1 cut(s) 1020
PctI GAATGC 2 cut(s) 247, 790
PfeI GAWTC 8 cut(s) 54, 60, 144, 255, 515, 562, 911, 929
PkrI GCNGC 3 cut(s) 166, 703, 1100
PleI GAGTC 3 cut(s) 95, 202, 407
PpsI GAGTC 3 cut(s) 95, 202, 407
Ppu21I YACGTR 1 cut(s) 371
PshAI GACNNNNGTC 1 cut(s) 607
Psp124BI GAGCTC 1 cut(s) 1032
Psp6I CCWGG 2 cut(s) 359, 858
PspEI GGTNACC 1 cut(s) 457
PspGI CCWGG 2 cut(s) 359, 858
PspN4I GGNNCC 4 cut(s) 10, 217, 742, 824
PvuII CAGCTG 1 cut(s) 1079
RsaI GTAC 3 cut(s) 119, 327, 824
RsaNI GTAC 3 cut(s) 118, 326, 823
SacI GAGCTC 1 cut(s) 1032
SapI GCTCTTC 1 cut(s) 1020
SaqAI TTAA 1 cut(s) 473
SatI GCNGC 3 cut(s) 165, 702, 1099
Sau3AI GATC 1 cut(s) 1073
ScaI AGTACT 1 cut(s) 327
SchI GAGTC 3 cut(s) 95, 203, 407
ScrFI CCNGG 2 cut(s) 361, 860
SduI GDGCHC 4 cut(s) 13, 141, 745, 1032
SfaNI GCATC 5 cut(s) 31, 139, 848, 890, 1051
SfuI TTCGAA 2 cut(s) 24, 927
Sse9I AATT 7 cut(s) 20, 45, 634, 714, 921, 939, 1053
SsiI CCGC 4 cut(s) 6, 333, 584, 704
SspMI CTAG 2 cut(s) 698, 819
SstI GAGCTC 1 cut(s) 1032
StyD4I CCNGG 2 cut(s) 359, 858
StyI CCWWGG 2 cut(s) 462, 818
TaaI ACNGT 3 cut(s) 117, 740, 950
TaiI ACGT 3 cut(s) 126, 373, 428
TaqI TCGA 6 cut(s) 24, 147, 174, 447, 927, 1094
TasI AATT 7 cut(s) 20, 45, 634, 714, 921, 939, 1053
TatI WGTACW 1 cut(s) 325
TfiI GAWTC 8 cut(s) 54, 60, 144, 255, 515, 562, 911, 929
Tru1I TTAA 1 cut(s) 473
Tru9I TTAA 1 cut(s) 473
TseFI GTSAC 3 cut(s) 421, 432, 1016
TseI GCWGC 3 cut(s) 164, 701, 1098
Tsp45I GTSAC 3 cut(s) 421, 432, 1016
TspDTI ATGAA 6 cut(s) 217, 327, 528, 726, 990, 1065
XapI RAATTY 1 cut(s) 20
XmaJI CCTAGG 1 cut(s) 818
XspI CTAG 2 cut(s) 698, 819
ZraI GACGTC 1 cut(s) 426
ZrmI AGTACT 1 cut(s) 327
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.