RLG00000006312

eukaryotic translation initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0007738
Physical Location & Seq
Reverse (-)
6193707 .. 6197557
3851 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006312

Sequence Viewer

Length: 999 bp
ATGAGCGGAGCCCCTTTGAAATTCGAAACCCCAATCCAAGATGCAATTTCCAGAATCCGATTCGCTCCAACCTCACACAACCTTCTCATCGCTTCTTGGGACTCCAAACTGCGACTGTACGACGTGGGAAGCTCGGTGCTCAGATTAGAAGCTCCGGCCGAAGCTGCACTACTCGATTGTTGCTTCCAGAACGAGGCGGTTGCGTTTAGCGCTGGCTCCGATGGCTTCATTAGAAGGTATGACTTGCATTCTGGAATCTGTGAGATGATTGGAAATCATGATGACATAGCAACCTGCATTGGATATTCTGATGAAACAAGCCAAGTACTTACAGCTGGTTTGGACAAGAAGATATTTGCCTGGGACACACGTATGGAGGAGGGTAAAAAATCTATGTTTGTAAAAAGTCTTGACTCAGAGGTGACGTCCATGTCACTCTCGGGTCTCGATATGATGGTAACCATGGGAGCGTTAATATATGTGTGTGATTTGCGCAACCTCCAAAAACCATATGAATCAAAAGTTTCAGATTTGAACACAACGATTATATGTGGAAGTGCGATTCCTAATGCTAAAGGATTTGCAGTTGGCTCAGCAGATGGAAGAGTGTCATTGGAGATTTGCCATCCATCTAATTCAGATAACATAAGATATATGTTCCGGTGTCATCCAAAGTCAACTGATAGAAGATATCATCTAGCAGCGGTGAATGAAATTGTGTTCAATCCTCTCATTTACGGTGCCCTTGTTACCGGTGATGATGAGGGTCATGTTATTGCATGGAATGCTCAAAGCAGAAAGCGGCTATTTCAGTTGCCTAGGTACCCAAATAGCGTGGCATCCTTATCGTATCACCACCAGGGACAACTTCTGGCCGTTGCATCAAGCTACACATATCGGCAAGCTATTGAATCAGAAGAGCTCCCTCAGATATTTATACATGAAATTGGCGATGCTAACATGAGATCAGCTGACATAGGAAGTTCAAGCCGCAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko05166 map04110 map04111 map05166
Pfam Domains
Protein Families
eIF-5a

Protein Analysis

333

Amino Acids

36.64

Weight (kDa)

5.69

Isoelectric Point (pI)

41.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WDR55 PF24796 12 - 227 7e-06 WDR55
Beta-prop_WDR5 PF25175 13 - 295 5.3e-17 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 14 - 122 7.4e-07 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 58 - 297 8.4e-11 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 63 - 205 2.7e-06 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 91 - 265 1.7e-06 CDC20/Fizzy WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 430
AatII GACGTC 1 cut(s) 428
Acc16I TGCGCA 1 cut(s) 494
Acc36I ACCTGC 1 cut(s) 302
Acc65I GGTACC 1 cut(s) 822
AccB1I GGYRCC 2 cut(s) 740, 822
AccBSI CCGCTC 1 cut(s) 6
AciI CCGC 5 cut(s) 6, 197, 704, 802, 991
AcoI YGGCCR 2 cut(s) 156, 873
AcsI RAATTY 1 cut(s) 20
AcyI GRCGYC 1 cut(s) 425
AfaI GTAC 3 cut(s) 119, 327, 824
AfeI AGCGCT 1 cut(s) 211
AfiI CCNNNNNNNGG 1 cut(s) 193
AflIII ACRYGT 1 cut(s) 368
AgeI ACCGGT 1 cut(s) 752
AgsI TTSAA 5 cut(s) 19, 535, 724, 911, 987
AjiI CACGTC 1 cut(s) 124
AjnI CCWGG 2 cut(s) 359, 858
AluBI AGCT 8 cut(s) 132, 152, 164, 335, 888, 905, 922, 971
AluI AGCT 8 cut(s) 132, 152, 164, 335, 888, 905, 922, 971
Alw21I GWGCWC 2 cut(s) 141, 924
Alw26I GTCTC 1 cut(s) 449
Ama87I CYCGRG 1 cut(s) 439
Aor51HI AGCGCT 1 cut(s) 211
AoxI GGCC 2 cut(s) 156, 873
ApeKI GCWGC 2 cut(s) 164, 701
ApoI RAATTY 1 cut(s) 20
AsiGI ACCGGT 1 cut(s) 752
Asp718I GGTACC 1 cut(s) 822
AspA2I CCTAGG 1 cut(s) 818
AspLEI GCGC 2 cut(s) 212, 495
AsuHPI GGTGA 4 cut(s) 433, 718, 767, 845
AsuII TTCGAA 1 cut(s) 24
AvaI CYCGRG 1 cut(s) 439
AvrII CCTAGG 1 cut(s) 818
BaeGI GKGCMC 1 cut(s) 745
BanI GGYRCC 2 cut(s) 740, 822
BanII GRGCYC 2 cut(s) 13, 924
BarI GAAGNNNNNNTAC 2 cut(s) 153, 185
Bbv12I GWGCWC 2 cut(s) 141, 924
BbvI GCAGC 2 cut(s) 151, 713
BccI CCATC 5 cut(s) 215, 448, 593, 633, 637
BceAI ACGGC 1 cut(s) 860
BcgI CGANNNNNNTGC 4 cut(s) 182, 216, 828, 862
BciT130I CCWGG 2 cut(s) 361, 860
BcoDI GTCTC 1 cut(s) 449
BfaI CTAG 2 cut(s) 698, 819
BfoI RGCGCY 1 cut(s) 213
BfuAI ACCTGC 1 cut(s) 302
BisI GCNGC 4 cut(s) 165, 702, 803, 991
BlnI CCTAGG 1 cut(s) 818
BlpI GCTNAGC 1 cut(s) 592
BlsI GCNGC 4 cut(s) 166, 703, 804, 992
BmcAI AGTACT 1 cut(s) 327
Bme1390I CCNGG 2 cut(s) 361, 860
BmeT110I CYCGRG 1 cut(s) 439
BmgBI CACGTC 1 cut(s) 124
BmiI GGNNCC 4 cut(s) 10, 217, 742, 824
BmrFI CCNGG 2 cut(s) 361, 860
BmsI GCATC 4 cut(s) 31, 848, 890, 943
Bpu1102I GCTNAGC 1 cut(s) 592
Bpu14I TTCGAA 1 cut(s) 24
BsaAI YACGTR 1 cut(s) 371
BsaHI GRCGYC 1 cut(s) 425
BsaI GGTCTC 1 cut(s) 449
BsaJI CCNNGG 4 cut(s) 360, 462, 818, 859
BsaWI WCCGGW 2 cut(s) 660, 752
Bsc4I CCNNNNNNNGG 1 cut(s) 193
Bse118I RCCGGY 1 cut(s) 752
BseBI CCWGG 2 cut(s) 361, 860
BseDI CCNNGG 4 cut(s) 360, 462, 818, 859
BseGI GGATG 3 cut(s) 625, 667, 839
BseLI CCNNNNNNNGG 1 cut(s) 193
BseMII CTCAG 4 cut(s) 154, 429, 606, 941
BseRI GAGGAG 1 cut(s) 392
BseSI GKGCMC 1 cut(s) 745
BseX3I CGGCCG 1 cut(s) 156
BseXI GCAGC 2 cut(s) 151, 713
BsgI GTGCAG 1 cut(s) 150
Bsh1285I CGRYCG 1 cut(s) 159
BshFI GGCC 2 cut(s) 158, 875
BshNI GGYRCC 2 cut(s) 740, 822
BshTI ACCGGT 1 cut(s) 752
BsiEI CGRYCG 1 cut(s) 159
BsiHKAI GWGCWC 2 cut(s) 141, 924
BsiHKCI CYCGRG 1 cut(s) 439
BsiSI CCGG 3 cut(s) 155, 661, 753
BslFI GGGAC 3 cut(s) 113, 377, 876
BslI CCNNNNNNNGG 1 cut(s) 193
BsmAI GTCTC 1 cut(s) 449
BsmFI GGGAC 3 cut(s) 113, 377, 876
BsmI GAATGC 2 cut(s) 247, 790
BsnI GGCC 2 cut(s) 158, 875
Bso31I GGTCTC 1 cut(s) 449
BsoBI CYCGRG 1 cut(s) 439
Bsp119I TTCGAA 1 cut(s) 24
Bsp1286I GDGCHC 4 cut(s) 13, 141, 745, 924
Bsp143I GATC 1 cut(s) 965
Bsp1720I GCTNAGC 1 cut(s) 592
Bsp19I CCATGG 1 cut(s) 462
BspACI CCGC 5 cut(s) 6, 197, 704, 802, 991
BspANI GGCC 2 cut(s) 158, 875
BspCNI CTCAG 4 cut(s) 153, 428, 605, 940
BspHI TCATGA 1 cut(s) 277
BspLI GGNNCC 4 cut(s) 10, 217, 742, 824
BspMI ACCTGC 1 cut(s) 302
BspQI GCTCTTC 1 cut(s) 912
BspT104I TTCGAA 1 cut(s) 24
BspT107I GGYRCC 2 cut(s) 740, 822
BspTNI GGTCTC 1 cut(s) 449
BsrBI CCGCTC 1 cut(s) 6
BsrFI RCCGGY 1 cut(s) 752
BssAI RCCGGY 1 cut(s) 752
BssECI CCNNGG 4 cut(s) 360, 462, 818, 859
BssMI GATC 1 cut(s) 965
BssNI GRCGYC 1 cut(s) 425
BssT1I CCWWGG 2 cut(s) 462, 818
Bst2UI CCWGG 2 cut(s) 361, 860
Bst4CI ACNGT 2 cut(s) 117, 740
Bst6I CTCTTC 2 cut(s) 598, 912
BstACI GRCGYC 1 cut(s) 425
BstAPI GCANNNNNTGC 1 cut(s) 785
BstBAI YACGTR 1 cut(s) 371
BstBI TTCGAA 1 cut(s) 24
BstC8I GCNNGC 2 cut(s) 214, 903
BstDEI CTNAG 4 cut(s) 140, 415, 592, 927
BstDSI CCRYGG 1 cut(s) 462
BstEII GGTNACC 1 cut(s) 457
BstF5I GGATG 3 cut(s) 625, 667, 839
BstH2I RGCGCY 1 cut(s) 213
BstHHI GCGC 2 cut(s) 212, 495
BstKTI GATC 1 cut(s) 968
BstMAI GTCTC 1 cut(s) 449
BstMBI GATC 1 cut(s) 965
BstMCI CGRYCG 1 cut(s) 159
BstMWI GCNNNNNNNGC 4 cut(s) 164, 209, 222, 785
BstNI CCWGG 2 cut(s) 361, 860
BstPI GGTNACC 1 cut(s) 457
BstSCI CCNGG 2 cut(s) 359, 858
BstSLI GKGCMC 1 cut(s) 745
BstV1I GCAGC 2 cut(s) 151, 713
BstZI CGGCCG 1 cut(s) 156
BsuRI GGCC 2 cut(s) 158, 875
BtgI CCRYGG 1 cut(s) 462
BtgZI GCGATG 2 cut(s) 73, 966
BtrI CACGTC 1 cut(s) 124
BtsCI GGATG 3 cut(s) 625, 667, 839
BveI ACCTGC 1 cut(s) 302
Cac8I GCNNGC 2 cut(s) 214, 903
CciI TCATGA 1 cut(s) 277
CfoI GCGC 2 cut(s) 212, 495
Cfr10I RCCGGY 1 cut(s) 752
Csp6I GTAC 3 cut(s) 118, 326, 823
CspAI ACCGGT 1 cut(s) 752
CspCI CAANNNNNGTGG 2 cut(s) 816, 851
CviAII CATG 7 cut(s) 278, 430, 463, 770, 780, 941, 961
CviQI GTAC 3 cut(s) 118, 326, 823
DdeI CTNAG 4 cut(s) 140, 415, 592, 927
DpnI GATC 1 cut(s) 967
DpnII GATC 1 cut(s) 965
DrdI GACNNNNNNGTC 1 cut(s) 430
DseDI GACNNNNNNGTC 1 cut(s) 430
EaeI YGGCCR 2 cut(s) 156, 873
EagI CGGCCG 1 cut(s) 156
Eam1104I CTCTTC 2 cut(s) 598, 912
EarI CTCTTC 2 cut(s) 598, 912
Ecl136II GAGCTC 1 cut(s) 922
EclXI CGGCCG 1 cut(s) 156
Eco130I CCWWGG 2 cut(s) 462, 818
Eco24I GRGCYC 2 cut(s) 13, 924
Eco31I GGTCTC 1 cut(s) 449
Eco32I GATATC 1 cut(s) 692
Eco47III AGCGCT 1 cut(s) 211
Eco52I CGGCCG 1 cut(s) 156
Eco53kI GAGCTC 1 cut(s) 922
Eco88I CYCGRG 1 cut(s) 439
Eco91I GGTNACC 1 cut(s) 457
EcoICRI GAGCTC 1 cut(s) 922
EcoO65I GGTNACC 1 cut(s) 457
EcoRII CCWGG 2 cut(s) 359, 858
EcoRV GATATC 1 cut(s) 692
EcoT14I CCWWGG 2 cut(s) 462, 818
EcoT38I GRGCYC 2 cut(s) 13, 924
ErhI CCWWGG 2 cut(s) 462, 818
FaeI CATG 7 cut(s) 281, 433, 466, 773, 783, 944, 964
FaqI GGGAC 3 cut(s) 113, 377, 876
FatI CATG 7 cut(s) 277, 429, 462, 769, 779, 940, 960
FauNDI CATATG 1 cut(s) 511
Fnu4HI GCNGC 4 cut(s) 165, 702, 803, 991
FokI GGATG 3 cut(s) 612, 654, 826
FriOI GRGCYC 2 cut(s) 13, 924
Fsp4HI GCNGC 4 cut(s) 165, 702, 803, 991
FspBI CTAG 2 cut(s) 698, 819
FspI TGCGCA 1 cut(s) 494
GlaI GCGC 2 cut(s) 211, 494
GluI GCNGC 4 cut(s) 165, 702, 803, 991
HaeII RGCGCY 1 cut(s) 213
HaeIII GGCC 2 cut(s) 158, 875
HapII CCGG 3 cut(s) 155, 661, 753
HhaI GCGC 2 cut(s) 212, 495
Hin1I GRCGYC 1 cut(s) 425
Hin1II CATG 7 cut(s) 281, 433, 466, 773, 783, 944, 964
Hin6I GCGC 2 cut(s) 210, 493
HinP1I GCGC 2 cut(s) 210, 493
HincII GTYRAC 1 cut(s) 678
HindII GTYRAC 1 cut(s) 678
HinfI GANTC 8 cut(s) 54, 60, 101, 255, 413, 515, 562, 911
HpaII CCGG 3 cut(s) 155, 661, 753
HphI GGTGA 4 cut(s) 433, 718, 767, 845
Hpy166II GTNNAC 1 cut(s) 678
Hpy188I TCNGA 9 cut(s) 59, 143, 220, 310, 418, 529, 640, 916, 930
Hpy188III TCNNGA 6 cut(s) 51, 187, 252, 278, 410, 446
Hpy8I GTNNAC 1 cut(s) 678
Hpy99I CGWCG 1 cut(s) 125
HpyAV CCTTC 2 cut(s) 92, 228
HpyCH4III ACNGT 2 cut(s) 117, 740
HpyCH4IV ACGT 3 cut(s) 123, 370, 425
HpyCH4V TGCA 7 cut(s) 44, 167, 247, 297, 584, 779, 881
HpyF10VI GCNNNNNNNGC 4 cut(s) 164, 209, 222, 785
HpyF3I CTNAG 4 cut(s) 140, 415, 592, 927
HpySE526I ACGT 3 cut(s) 123, 370, 425
Hsp92I GRCGYC 1 cut(s) 425
Hsp92II CATG 7 cut(s) 281, 433, 466, 773, 783, 944, 964
HspAI GCGC 2 cut(s) 210, 493
KpnI GGTACC 1 cut(s) 826
Kzo9I GATC 1 cut(s) 965
LguI GCTCTTC 1 cut(s) 912
LmnI GCTCC 6 cut(s) 8, 70, 157, 221, 467, 927
Lsp1109I GCAGC 2 cut(s) 151, 713
LweI GCATC 4 cut(s) 31, 848, 890, 943
MaeI CTAG 2 cut(s) 698, 819
MaeII ACGT 3 cut(s) 123, 370, 425
MaeIII GTNAC 4 cut(s) 421, 432, 457, 748
MalI GATC 1 cut(s) 967
MbiI CCGCTC 1 cut(s) 6
MboI GATC 1 cut(s) 965
MboII GAAGA 4 cut(s) 361, 615, 699, 929
MhlI GDGCHC 4 cut(s) 13, 141, 745, 924
MluCI AATT 5 cut(s) 20, 45, 634, 714, 945
MlyI GAGTC 2 cut(s) 95, 407
MmeI TCCRAC 1 cut(s) 92
MnlI CCTC 9 cut(s) 82, 187, 370, 373, 412, 509, 738, 757, 936
MseI TTAA 1 cut(s) 473
MslI CAYNNNNRTG 1 cut(s) 371
MspA1I CMGCKG 3 cut(s) 335, 704, 971
MspI CCGG 3 cut(s) 155, 661, 753
MspR9I CCNGG 2 cut(s) 361, 860
Mva1269I GAATGC 2 cut(s) 247, 790
MvaI CCWGG 2 cut(s) 361, 860
MwoI GCNNNNNNNGC 4 cut(s) 164, 209, 222, 785
NcoI CCATGG 1 cut(s) 462
NdeI CATATG 1 cut(s) 511
NdeII GATC 1 cut(s) 965
NlaIII CATG 7 cut(s) 281, 433, 466, 773, 783, 944, 964
NlaIV GGNNCC 4 cut(s) 10, 217, 742, 824
NmuCI GTSAC 2 cut(s) 421, 432
NsbI TGCGCA 1 cut(s) 494
NspV TTCGAA 1 cut(s) 24
PagI TCATGA 1 cut(s) 277
PciSI GCTCTTC 1 cut(s) 912
PctI GAATGC 2 cut(s) 247, 790
PfeI GAWTC 6 cut(s) 54, 60, 255, 515, 562, 911
PinAI ACCGGT 1 cut(s) 752
PkrI GCNGC 4 cut(s) 166, 703, 804, 992
PleI GAGTC 2 cut(s) 95, 407
PpsI GAGTC 2 cut(s) 95, 407
Ppu21I YACGTR 1 cut(s) 371
Psp124BI GAGCTC 1 cut(s) 924
Psp6I CCWGG 2 cut(s) 359, 858
PspEI GGTNACC 1 cut(s) 457
PspGI CCWGG 2 cut(s) 359, 858
PspN4I GGNNCC 4 cut(s) 10, 217, 742, 824
PvuII CAGCTG 2 cut(s) 335, 971
RsaI GTAC 3 cut(s) 119, 327, 824
RsaNI GTAC 3 cut(s) 118, 326, 823
RseI CAYNNNNRTG 1 cut(s) 371
SacI GAGCTC 1 cut(s) 924
SapI GCTCTTC 1 cut(s) 912
SaqAI TTAA 1 cut(s) 473
SatI GCNGC 4 cut(s) 165, 702, 803, 991
Sau3AI GATC 1 cut(s) 965
ScaI AGTACT 1 cut(s) 327
SchI GAGTC 2 cut(s) 95, 407
ScrFI CCNGG 2 cut(s) 361, 860
SduI GDGCHC 4 cut(s) 13, 141, 745, 924
SfaNI GCATC 4 cut(s) 31, 848, 890, 943
SfuI TTCGAA 1 cut(s) 24
SmiMI CAYNNNNRTG 1 cut(s) 371
Sse9I AATT 5 cut(s) 20, 45, 634, 714, 945
SsiI CCGC 5 cut(s) 6, 197, 704, 802, 991
SspMI CTAG 2 cut(s) 698, 819
SstI GAGCTC 1 cut(s) 924
StyD4I CCNGG 2 cut(s) 359, 858
StyI CCWWGG 2 cut(s) 462, 818
TaaI ACNGT 2 cut(s) 117, 740
TaiI ACGT 3 cut(s) 126, 373, 428
TaqI TCGA 3 cut(s) 24, 174, 447
TasI AATT 5 cut(s) 20, 45, 634, 714, 945
TatI WGTACW 1 cut(s) 325
TauI GCSGC 2 cut(s) 805, 993
TfiI GAWTC 6 cut(s) 54, 60, 255, 515, 562, 911
Tru1I TTAA 1 cut(s) 473
Tru9I TTAA 1 cut(s) 473
TseFI GTSAC 2 cut(s) 421, 432
TseI GCWGC 2 cut(s) 164, 701
Tsp45I GTSAC 2 cut(s) 421, 432
TspDTI ATGAA 5 cut(s) 217, 327, 528, 726, 957
XapI RAATTY 1 cut(s) 20
XmaJI CCTAGG 1 cut(s) 818
XspI CTAG 2 cut(s) 698, 819
ZraI GACGTC 1 cut(s) 426
ZrmI AGTACT 1 cut(s) 327
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.