Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0007738

Physical Location & Seq

Genomic Coordinates Forward (+)

4619525 .. 4622395

Gene Structure 2871 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1066300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 993 bp

ATGAGCGGAGCTCCTTTGAAGTTCGAAAACCCGATCCGAGATGCAATTTCCAGAATCCGATTCGCCCCGAAATCCGACAACCTCCTCATCTCCTCTTGGGACTCTAGCTTGAGATTGTACGACGTCGGAAGCTCACAGCTCAGATTAGAAGCTCCGTCGGAAGCGGCGCTCCTCGATTGCTGTTTCCAGGACGAGTCCGTCGCGTTTGCTGCCGGTTCTGATGGCGTCATCAGAAGGTACGATTTACATTCTGGAATTCATGATTCGATTGGGAATCACGATGACATGTCGACATGCGTTGGATATTCGGAAGAAACCAGCCAACTAATCACTGCTGGTTTGGACAGGAAGATATTGTCTTTGGACACGCGTACGGAGAAGGGTTTTGTGTTTGCAAGTAATCTCGATTCGGAGGTGATGTCCATGTCACTCTCAGGTCTTGATATGATGGTAGCCATTGGATCATCAATATATGTGTATGATTTGCGAAACCCAGAAAAGCCAATTCAATCAAAAGATTCACATATGGATGTTAAGATTGTATGTGTTAGCTCAATTCCATATGCTAAAGGATTTGCGGTTGGCTCGATAGATGGACGTGTAGCACTGGAGATTTCCTGTCCGTCTAGTTCAGATGACATCAGATATATGTTCCGGTGTCATCCGAAGTCAACTGATAAGAGATATCACCTAGAATCGGTGAATGACATTGTATTCAATCCACTCATGTACGGCGCCTTTGTTACGGGTGACAACGATGGTTATGTTACTGCATGGGATGCTAAAAGCAAGAAGAGGCTATTTCAGTTGCCTAGATACCCAAATAGTGTGGCATCCCTATCGTACAACCACAGGGGACAACTTCTGGCTGTTGCATCAAGCTACACATATCAAGAAGCTAATGAAATAGAAGAGCTTCCTCAGGTATTCGTTCATGAAGTTGGCAATGACAACATGAAATCAGTATCTGTTGGAAGTTCGAGCAGGAACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000075 GO:0000151 GO:0000278 GO:0000775 GO:0000776 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0007049 GO:0007088 GO:0007093 GO:0007094 GO:0007346 GO:0008150 GO:0009524 GO:0009987 GO:0010564 GO:0010639 GO:0010948 GO:0010965 GO:0022402 GO:0030071 GO:0031461 GO:0031577 GO:0032182 GO:0032991 GO:0033043 GO:0033044 GO:0033045 GO:0033046 GO:0033047 GO:0033048 GO:0033597 GO:0043130 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045786 GO:0045839 GO:0045841 GO:0045930 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051128 GO:0051129 GO:0051726 GO:0051783 GO:0051784 GO:0051983 GO:0051985 GO:0065007 GO:0071173 GO:0071174 GO:0080008 GO:0098687 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902099 GO:1902100 GO:1902494 GO:1903047 GO:1905818 GO:1905819 GO:1990234 GO:1990298 GO:2000816 GO:2001251

KEGG Pathways

Metabolic & Signaling

ko04110 ko04111 ko05166 map04110 map04111 map05166

Pfam Domains

Protein Families

WD40

Protein Analysis

331

Amino Acids

36.42

Weight (kDa)

5.21

Isoelectric Point (pI)

46.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_WDR5	PF25175	13 - 151	1.5e-08	WDR5 beta-propeller domain
WDR55	PF24796	13 - 156	2.9e-07	WDR55
Beta-prop_THOC3	PF25174	15 - 202	4.8e-07	THOC3 beta-propeller domain
WD40_Prp19	PF24814	58 - 295	6.2e-09	Prp19 WD40 domain
WD40_CDC20-Fz	PF24807	92 - 295	3.3e-07	CDC20/Fizzy WD40 domain
Beta-prop_THOC3	PF25174	225 - 294	1.2e-06	THOC3 beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007738)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G69400 AT1G69400
fragaria_vesca	FvH4_4g30600 FvH4_4g30600 FvH4_4g30600
malus_domestica	MD13G1067000.v1.1 MD13G1067200.v1.1 MD16G1066300.v1.1
prunus_persica	Prupe.1G281000_v2.0.a1 Prupe.1G281000_v2.0.a1 Prupe.1G281000_v2.0.a1
pyrus_communis	pycom16g05770
rosa_chinensis	RchiOBHm_Chr4g0439001
rosa_laevigata	RLG00000006312
rosa_multiflora	Rmu_sc0005887.1_g000008 Rmu_sc0023005.1_g000006
rosa_roxburghii	Rroxscaffold_5G00379990
rosa_rugosa	Rorug04G0315000.1
rosa_samantha	Rh4AG365600 Rh4BG376800 Rh4CG390900 Rh4DG371500
rosa_wichuraiana	Rw0G007390 Rw0G016940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	197
AatII	GACGTC	1 cut(s)	126
AccB1I	GGYRCC	1 cut(s)	734
AccBSI	CCGCTC	1 cut(s)	6
AccI	GTMKAC	1 cut(s)	290
AccII	CGCG	2 cut(s)	203, 370
AciI	CCGC	3 cut(s)	6, 164, 578
AclWI	GGATC	2 cut(s)	28, 469
AcsI	RAATTY	1 cut(s)	255
AcyI	GRCGYC	3 cut(s)	123, 225, 735
AfaI	GTAC	5 cut(s)	119, 239, 373, 731, 845
AfiI	CCNNNNNNNGG	1 cut(s)	697
AflIII	ACRYGT	3 cut(s)	285, 368, 598
AgsI	TTSAA	3 cut(s)	19, 509, 718
AjiI	CACGTC	1 cut(s)	599
AjnI	CCWGG	1 cut(s)	186
AluBI	AGCT	9 cut(s)	11, 108, 132, 139, 152, 552, 882, 899, 916
AluI	AGCT	9 cut(s)	11, 108, 132, 139, 152, 552, 882, 899, 916
Alw21I	GWGCWC	1 cut(s)	13
AlwI	GGATC	2 cut(s)	28, 469
AlwNI	CAGNNNCTG	1 cut(s)	968
ApeKI	GCWGC	1 cut(s)	209
ApoI	RAATTY	1 cut(s)	255
Asp700I	GAANNNNTTC	1 cut(s)	915
AspLEI	GCGC	2 cut(s)	169, 737
AsuHPI	GGTGA	4 cut(s)	427, 680, 712, 761
AsuII	TTCGAA	1 cut(s)	24
AxyI	CCTNAGG	1 cut(s)	921
BanI	GGYRCC	1 cut(s)	734
BanII	GRGCYC	1 cut(s)	13
Bbv12I	GWGCWC	1 cut(s)	13
BbvI	GCAGC	1 cut(s)	196
BccI	CCATC	4 cut(s)	215, 442, 587, 752
BceAI	ACGGC	1 cut(s)	748
BcgI	CGANNNNNNTGC	2 cut(s)	822, 856
BciT130I	CCWGG	1 cut(s)	188
BfaI	CTAG	4 cut(s)	105, 627, 692, 813
BfoI	RGCGCY	2 cut(s)	170, 738
BisI	GCNGC	2 cut(s)	165, 210
BlsI	GCNGC	2 cut(s)	166, 211
Bme1390I	CCNGG	1 cut(s)	188
BmgBI	CACGTC	1 cut(s)	599
BmiI	GGNNCC	1 cut(s)	736
BmrFI	CCNGG	1 cut(s)	188
BmsI	GCATC	4 cut(s)	31, 769, 842, 884
BplI	GAGNNNNNCTC	1 cut(s)	27
BpmI	CTGGAG	1 cut(s)	629
Bpu14I	TTCGAA	1 cut(s)	24
BpuEI	CTTGAG	1 cut(s)	130
BsaHI	GRCGYC	3 cut(s)	123, 225, 735
BsaWI	WCCGGW	1 cut(s)	654
BsaXI	ACNNNNNCTCC	2 cut(s)	404, 434
Bsc4I	CCNNNNNNNGG	1 cut(s)	697
Bse118I	RCCGGY	1 cut(s)	212
Bse1I	ACTGG	1 cut(s)	612
Bse21I	CCTNAGG	1 cut(s)	921
Bse3DI	GCAATG	1 cut(s)	952
BseBI	CCWGG	1 cut(s)	188
BseGI	GGATG	4 cut(s)	535, 661, 784, 833
BseLI	CCNNNNNNNGG	1 cut(s)	697
BseMI	GCAATG	1 cut(s)	952
BseMII	CTCAG	3 cut(s)	154, 447, 935
BseNI	ACTGG	1 cut(s)	612
BseRI	GAGGAG	3 cut(s)	74, 82, 161
BseXI	GCAGC	1 cut(s)	196
Bsh1236I	CGCG	2 cut(s)	203, 370
BshNI	GGYRCC	1 cut(s)	734
BsiHKAI	GWGCWC	1 cut(s)	13
BsiSI	CCGG	2 cut(s)	213, 655
BsiWI	CGTACG	1 cut(s)	371
BslFI	GGGAC	2 cut(s)	113, 870
BslI	CCNNNNNNNGG	1 cut(s)	697
BsmFI	GGGAC	2 cut(s)	113, 870
Bsp119I	TTCGAA	1 cut(s)	24
Bsp1286I	GDGCHC	1 cut(s)	13
Bsp143I	GATC	2 cut(s)	33, 461
BspACI	CCGC	3 cut(s)	6, 164, 578
BspCNI	CTCAG	3 cut(s)	153, 446, 934
BspFNI	CGCG	2 cut(s)	203, 370
BspHI	TCATGA	2 cut(s)	259, 934
BspLI	GGNNCC	1 cut(s)	736
BspPI	GGATC	2 cut(s)	28, 469
BspQI	GCTCTTC	1 cut(s)	906
BspT104I	TTCGAA	1 cut(s)	24
BspT107I	GGYRCC	1 cut(s)	734
BsrBI	CCGCTC	1 cut(s)	6
BsrDI	GCAATG	1 cut(s)	952
BsrFI	RCCGGY	1 cut(s)	212
BsrI	ACTGG	1 cut(s)	612
BssAI	RCCGGY	1 cut(s)	212
BssMI	GATC	2 cut(s)	33, 461
BssNI	GRCGYC	3 cut(s)	123, 225, 735
Bst2UI	CCWGG	1 cut(s)	188
Bst6I	CTCTTC	2 cut(s)	788, 906
BstACI	GRCGYC	3 cut(s)	123, 225, 735
BstAPI	GCANNNNNTGC	1 cut(s)	779
BstBI	TTCGAA	1 cut(s)	24
BstDEI	CTNAG	3 cut(s)	140, 433, 921
BstF5I	GGATG	4 cut(s)	535, 661, 784, 833
BstFNI	CGCG	2 cut(s)	203, 370
BstH2I	RGCGCY	2 cut(s)	170, 738
BstHHI	GCGC	2 cut(s)	169, 737
BstKTI	GATC	2 cut(s)	36, 464
BstMBI	GATC	2 cut(s)	33, 461
BstMWI	GCNNNNNNNGC	2 cut(s)	209, 779
BstNI	CCWGG	1 cut(s)	188
BstNSI	RCATGY	2 cut(s)	289, 297
BstSCI	CCNGG	1 cut(s)	186
BstUI	CGCG	2 cut(s)	203, 370
BstV1I	GCAGC	1 cut(s)	196
Bsu36I	CCTNAGG	1 cut(s)	921
BtrI	CACGTC	1 cut(s)	599
BtsCI	GGATG	4 cut(s)	535, 661, 784, 833
BtsI	GCAGTG	1 cut(s)	330
BtsIMutI	CAGTG	2 cut(s)	330, 605
CaiI	CAGNNNCTG	1 cut(s)	968
CciI	TCATGA	2 cut(s)	259, 934
CfoI	GCGC	2 cut(s)	169, 737
Cfr10I	RCCGGY	1 cut(s)	212
CseI	GACGC	1 cut(s)	214
Csp6I	GTAC	5 cut(s)	118, 238, 372, 730, 844
CspCI	CAANNNNNGTGG	2 cut(s)	810, 845
CviAII	CATG	8 cut(s)	260, 286, 294, 424, 727, 774, 935, 955
CviQI	GTAC	5 cut(s)	118, 238, 372, 730, 844
DdeI	CTNAG	3 cut(s)	140, 433, 921
DinI	GGCGCC	1 cut(s)	736
DpnI	GATC	2 cut(s)	35, 463
DpnII	GATC	2 cut(s)	33, 461
DrdI	GACNNNNNNGTC	1 cut(s)	197
DseDI	GACNNNNNNGTC	1 cut(s)	197
Eam1104I	CTCTTC	2 cut(s)	788, 906
EarI	CTCTTC	2 cut(s)	788, 906
Ecl136II	GAGCTC	1 cut(s)	11
Eco24I	GRGCYC	1 cut(s)	13
Eco32I	GATATC	1 cut(s)	686
Eco53kI	GAGCTC	1 cut(s)	11
Eco81I	CCTNAGG	1 cut(s)	921
EcoICRI	GAGCTC	1 cut(s)	11
EcoRI	GAATTC	1 cut(s)	255
EcoRII	CCWGG	1 cut(s)	186
EcoRV	GATATC	1 cut(s)	686
EcoT38I	GRGCYC	1 cut(s)	13
EgeI	GGCGCC	1 cut(s)	736
EheI	GGCGCC	1 cut(s)	736
FaeI	CATG	8 cut(s)	263, 289, 297, 427, 730, 777, 938, 958
FaqI	GGGAC	2 cut(s)	113, 870
FatI	CATG	8 cut(s)	259, 285, 293, 423, 726, 773, 934, 954
FauNDI	CATATG	2 cut(s)	525, 562
FblI	GTMKAC	1 cut(s)	290
Fnu4HI	GCNGC	2 cut(s)	165, 210
FokI	GGATG	4 cut(s)	542, 648, 791, 820
FriOI	GRGCYC	1 cut(s)	13
Fsp4HI	GCNGC	2 cut(s)	165, 210
FspBI	CTAG	4 cut(s)	105, 627, 692, 813
GlaI	GCGC	2 cut(s)	168, 736
GluI	GCNGC	2 cut(s)	165, 210
GsuI	CTGGAG	1 cut(s)	629
HaeII	RGCGCY	2 cut(s)	170, 738
HapII	CCGG	2 cut(s)	213, 655
HgaI	GACGC	1 cut(s)	214
HhaI	GCGC	2 cut(s)	169, 737
Hin1I	GRCGYC	3 cut(s)	123, 225, 735
Hin1II	CATG	8 cut(s)	263, 289, 297, 427, 730, 777, 938, 958
Hin6I	GCGC	2 cut(s)	167, 735
HinP1I	GCGC	2 cut(s)	167, 735
HincII	GTYRAC	2 cut(s)	291, 672
HindII	GTYRAC	2 cut(s)	291, 672
HinfI	GANTC	9 cut(s)	54, 60, 101, 194, 263, 274, 407, 518, 695
HpaII	CCGG	2 cut(s)	213, 655
HphI	GGTGA	4 cut(s)	427, 680, 712, 761
Hpy166II	GTNNAC	2 cut(s)	291, 672
Hpy188III	TCNNGA	8 cut(s)	51, 252, 260, 278, 404, 440, 893, 935
Hpy8I	GTNNAC	2 cut(s)	291, 672
Hpy99I	CGWCG	4 cut(s)	125, 128, 160, 203
HpyAV	CCTTC	2 cut(s)	228, 373
HpyCH4IV	ACGT	2 cut(s)	123, 598
HpyCH4V	TGCA	4 cut(s)	44, 395, 773, 875
HpyF10VI	GCNNNNNNNGC	2 cut(s)	209, 779
HpyF3I	CTNAG	3 cut(s)	140, 433, 921
HpySE526I	ACGT	2 cut(s)	123, 598
Hsp92I	GRCGYC	3 cut(s)	123, 225, 735
Hsp92II	CATG	8 cut(s)	263, 289, 297, 427, 730, 777, 938, 958
HspAI	GCGC	2 cut(s)	167, 735
KasI	GGCGCC	1 cut(s)	734
Kzo9I	GATC	2 cut(s)	33, 461
LguI	GCTCTTC	1 cut(s)	906
LmnI	GCTCC	4 cut(s)	8, 16, 157, 174
Lsp1109I	GCAGC	1 cut(s)	196
LweI	GCATC	4 cut(s)	31, 769, 842, 884
MaeI	CTAG	4 cut(s)	105, 627, 692, 813
MaeII	ACGT	2 cut(s)	123, 598
MaeIII	GTNAC	4 cut(s)	426, 742, 749, 766
MalI	GATC	2 cut(s)	35, 463
MbiI	CCGCTC	1 cut(s)	6
MboI	GATC	2 cut(s)	33, 461
MboII	GAAGA	4 cut(s)	323, 361, 805, 923
MhlI	GDGCHC	1 cut(s)	13
MluCI	AATT	4 cut(s)	45, 255, 504, 555
MluI	ACGCGT	1 cut(s)	368
Mly113I	GGCGCC	1 cut(s)	735
MlyI	GAGTC	2 cut(s)	95, 203
MmeI	TCCRAC	5 cut(s)	99, 106, 138, 280, 952
MnlI	CCTC	7 cut(s)	92, 95, 103, 182, 406, 789, 930
MroXI	GAANNNNTTC	1 cut(s)	915
MseI	TTAA	1 cut(s)	534
MslI	CAYNNNNRTG	1 cut(s)	528
MspI	CCGG	2 cut(s)	213, 655
MspR9I	CCNGG	1 cut(s)	188
MvaI	CCWGG	1 cut(s)	188
MvnI	CGCG	2 cut(s)	203, 370
MwoI	GCNNNNNNNGC	2 cut(s)	209, 779
NarI	GGCGCC	1 cut(s)	735
NdeI	CATATG	2 cut(s)	525, 562
NdeII	GATC	2 cut(s)	33, 461
NlaIII	CATG	8 cut(s)	263, 289, 297, 427, 730, 777, 938, 958
NlaIV	GGNNCC	1 cut(s)	736
NmuCI	GTSAC	2 cut(s)	426, 749
NspI	RCATGY	2 cut(s)	289, 297
NspV	TTCGAA	1 cut(s)	24
PagI	TCATGA	2 cut(s)	259, 934
PciI	ACATGT	1 cut(s)	285
PciSI	GCTCTTC	1 cut(s)	906
PdmI	GAANNNNTTC	1 cut(s)	915
PfeI	GAWTC	7 cut(s)	54, 60, 263, 274, 407, 518, 695
Pfl23II	CGTACG	1 cut(s)	371
PfoI	TCCNGGA	1 cut(s)	186
PkrI	GCNGC	2 cut(s)	166, 211
PleI	GAGTC	2 cut(s)	95, 202
PluTI	GGCGCC	1 cut(s)	738
PpsI	GAGTC	2 cut(s)	95, 202
PscI	ACATGT	1 cut(s)	285
Psp124BI	GAGCTC	1 cut(s)	13
Psp6I	CCWGG	1 cut(s)	186
PspGI	CCWGG	1 cut(s)	186
PspLI	CGTACG	1 cut(s)	371
PspN4I	GGNNCC	1 cut(s)	736
PstNI	CAGNNNCTG	1 cut(s)	968
RsaI	GTAC	5 cut(s)	119, 239, 373, 731, 845
RsaNI	GTAC	5 cut(s)	118, 238, 372, 730, 844
RseI	CAYNNNNRTG	1 cut(s)	528
SacI	GAGCTC	1 cut(s)	13
SalI	GTCGAC	1 cut(s)	289
SapI	GCTCTTC	1 cut(s)	906
SaqAI	TTAA	1 cut(s)	534
SatI	GCNGC	2 cut(s)	165, 210
Sau3AI	GATC	2 cut(s)	33, 461
SchI	GAGTC	2 cut(s)	95, 203
ScrFI	CCNGG	1 cut(s)	188
SduI	GDGCHC	1 cut(s)	13
SfaNI	GCATC	4 cut(s)	31, 769, 842, 884
SfoI	GGCGCC	1 cut(s)	736
SfuI	TTCGAA	1 cut(s)	24
SmiMI	CAYNNNNRTG	1 cut(s)	528
SmlI	CTYRAG	1 cut(s)	109
SmoI	CTYRAG	1 cut(s)	109
Sse9I	AATT	4 cut(s)	45, 255, 504, 555
SsiI	CCGC	3 cut(s)	6, 164, 578
SspDI	GGCGCC	1 cut(s)	734
SspMI	CTAG	4 cut(s)	105, 627, 692, 813
SstI	GAGCTC	1 cut(s)	13
StyD4I	CCNGG	1 cut(s)	186
TaiI	ACGT	2 cut(s)	126, 601
TaqI	TCGA	7 cut(s)	24, 174, 266, 290, 405, 587, 980
TasI	AATT	4 cut(s)	45, 255, 504, 555
TauI	GCSGC	1 cut(s)	167
TfiI	GAWTC	7 cut(s)	54, 60, 263, 274, 407, 518, 695
Tru1I	TTAA	1 cut(s)	534
Tru9I	TTAA	1 cut(s)	534
TscAI	CASTG	2 cut(s)	337, 612
TseFI	GTSAC	2 cut(s)	426, 749
TseI	GCWGC	1 cut(s)	209
Tsp45I	GTSAC	2 cut(s)	426, 749
TspDTI	ATGAA	5 cut(s)	248, 918, 923, 951, 971
TspGWI	ACGGA	4 cut(s)	144, 187, 389, 612
TspRI	CASTG	2 cut(s)	337, 612
XapI	RAATTY	1 cut(s)	255
XceI	RCATGY	2 cut(s)	289, 297
XmiI	GTMKAC	1 cut(s)	290
XmnI	GAANNNNTTC	1 cut(s)	915
XspI	CTAG	4 cut(s)	105, 627, 692, 813
ZraI	GACGTC	1 cut(s)	124

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.