Rh4BG376800

eukaryotic translation initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0007738
Physical Location & Seq
Forward (+)
53214476 .. 53217917
3442 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG376800.1

Sequence Viewer

Length: 825 bp
ATGATTGGAAACCATGATGACATAGCAACCTGCATTGGATATTCTGATGAAACAAGCCAAGTACTTACCGCTGGTTTGGACAAGAAGATATTTGCCTGGGATGCACGTATGGAGGAGGGTAAAAAATCTGTGTTTGTAAAAAGTCTTGACTCAGAGGTGACGTCCATGTCACTCTCGGGTCTCGATATGATGGTAACCATGGGAGCTTTAATATATGTGTGTGATTTGCGCAACCTCCAAAAACCATATGAATCAAAAGTTTCAGATTTGAACACAACGATTATATGTGGAAGTGCGATTCCTAATGCTAAAGGATTTGCGGTTGGCTCAGCAGATGGACGAGTGTCATTGGAGATTTTCCGTCCATCTAATTCAGATAACATCAGATATATGTTCCGGTGTCATCCGAAGTCAACTGATAGAAGATATCATCTAGCAGCGGTGAATGAAATTGTGTTCAATCCTCTCATTTACGGTGCCCTTCTTACTGGAGATGATGAGGGTCATGTTATTGCATGGAATGCTCAAAGCAGAAAGAGGCTATTTCAGTTGCCTAGGTACCCAAATAGCGTGGCATCCTTATCGTATCACCACCAGGGACAACTTCTGGCCGTTGCATCAAGCTACACATATCGGCAAGCTATTGAATCGTTAGTAATTTTCTACTGTCATCTACTTTTATCTGCACCATTACTATCTTTGTTGTTTTATTCTCCTTCATGTCCACTTCCTTGTGACAGAGAAGAGCTCCCTCAGATATTTATACATGAAATTGGCGATGCTAACATGAGATCAGCTGACATAGGAAGTTTGAGCCGCAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko05166 map04110 map04111 map05166
Pfam Domains
Protein Families
eIF-5a

Protein Analysis

274

Amino Acids

30.44

Weight (kDa)

6.06

Isoelectric Point (pI)

43.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR5 PF25175 4 - 208 7.1e-08 WDR5 beta-propeller domain
WD40_CDC20-Fz PF24807 5 - 176 8.4e-06 CDC20/Fizzy WD40 domain
WD40_Prp19 PF24814 5 - 208 2.6e-07 Prp19 WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 166
AatII GACGTC 1 cut(s) 164
Acc16I TGCGCA 1 cut(s) 230
Acc36I ACCTGC 1 cut(s) 38
Acc65I GGTACC 1 cut(s) 558
AccB1I GGYRCC 2 cut(s) 476, 558
AciI CCGC 4 cut(s) 69, 320, 440, 817
AcoI YGGCCR 1 cut(s) 609
AcyI GRCGYC 1 cut(s) 161
AfaI GTAC 2 cut(s) 63, 560
AgsI TTSAA 3 cut(s) 271, 460, 647
AjnI CCWGG 2 cut(s) 95, 594
AluBI AGCT 5 cut(s) 206, 624, 641, 748, 797
AluI AGCT 5 cut(s) 206, 624, 641, 748, 797
Alw21I GWGCWC 1 cut(s) 750
Alw26I GTCTC 1 cut(s) 185
Ama87I CYCGRG 1 cut(s) 175
AoxI GGCC 1 cut(s) 609
ApeKI GCWGC 1 cut(s) 437
Asp718I GGTACC 1 cut(s) 558
AspA2I CCTAGG 1 cut(s) 554
AspLEI GCGC 1 cut(s) 231
AsuHPI GGTGA 3 cut(s) 169, 454, 581
AvaI CYCGRG 1 cut(s) 175
AvrII CCTAGG 1 cut(s) 554
BaeGI GKGCMC 1 cut(s) 481
BanI GGYRCC 2 cut(s) 476, 558
BanII GRGCYC 1 cut(s) 750
Bbv12I GWGCWC 1 cut(s) 750
BbvI GCAGC 1 cut(s) 449
BccI CCATC 3 cut(s) 184, 329, 373
BceAI ACGGC 1 cut(s) 596
BcgI CGANNNNNNTGC 2 cut(s) 564, 598
BciT130I CCWGG 2 cut(s) 97, 596
BcoDI GTCTC 1 cut(s) 185
BfaI CTAG 2 cut(s) 434, 555
BfuAI ACCTGC 1 cut(s) 38
BisI GCNGC 2 cut(s) 438, 817
BlnI CCTAGG 1 cut(s) 554
BlpI GCTNAGC 1 cut(s) 328
BlsI GCNGC 2 cut(s) 439, 818
BmcAI AGTACT 1 cut(s) 63
Bme1390I CCNGG 2 cut(s) 97, 596
BmeT110I CYCGRG 1 cut(s) 175
BmiI GGNNCC 2 cut(s) 478, 560
BmrFI CCNGG 2 cut(s) 97, 596
BmsI GCATC 4 cut(s) 91, 584, 626, 769
BoxI GACNNNNGTC 1 cut(s) 343
BplI GAGNNNNNCTC 2 cut(s) 732, 764
BpmI CTGGAG 1 cut(s) 510
Bpu1102I GCTNAGC 1 cut(s) 328
BsaAI YACGTR 1 cut(s) 107
BsaHI GRCGYC 1 cut(s) 161
BsaI GGTCTC 1 cut(s) 185
BsaJI CCNNGG 4 cut(s) 96, 198, 554, 595
BsaWI WCCGGW 1 cut(s) 396
Bse1I ACTGG 1 cut(s) 493
BseBI CCWGG 2 cut(s) 97, 596
BseDI CCNNGG 4 cut(s) 96, 198, 554, 595
BseGI GGATG 3 cut(s) 106, 403, 575
BseMII CTCAG 3 cut(s) 165, 342, 767
BseNI ACTGG 1 cut(s) 493
BseRI GAGGAG 1 cut(s) 128
BseSI GKGCMC 1 cut(s) 481
BseXI GCAGC 1 cut(s) 449
BsgI GTGCAG 1 cut(s) 669
BshFI GGCC 1 cut(s) 611
BshNI GGYRCC 2 cut(s) 476, 558
BsiHKAI GWGCWC 1 cut(s) 750
BsiHKCI CYCGRG 1 cut(s) 175
BsiSI CCGG 1 cut(s) 397
BslFI GGGAC 1 cut(s) 612
BsmAI GTCTC 1 cut(s) 185
BsmFI GGGAC 1 cut(s) 612
BsmI GAATGC 1 cut(s) 526
BsnI GGCC 1 cut(s) 611
Bso31I GGTCTC 1 cut(s) 185
BsoBI CYCGRG 1 cut(s) 175
Bsp1286I GDGCHC 2 cut(s) 481, 750
Bsp143I GATC 1 cut(s) 791
Bsp1720I GCTNAGC 1 cut(s) 328
Bsp19I CCATGG 1 cut(s) 198
BspACI CCGC 4 cut(s) 69, 320, 440, 817
BspANI GGCC 1 cut(s) 611
BspCNI CTCAG 3 cut(s) 164, 341, 766
BspLI GGNNCC 2 cut(s) 478, 560
BspMI ACCTGC 1 cut(s) 38
BspQI GCTCTTC 1 cut(s) 738
BspT107I GGYRCC 2 cut(s) 476, 558
BspTNI GGTCTC 1 cut(s) 185
BsrI ACTGG 1 cut(s) 493
BssECI CCNNGG 4 cut(s) 96, 198, 554, 595
BssMI GATC 1 cut(s) 791
BssNI GRCGYC 1 cut(s) 161
BssT1I CCWWGG 2 cut(s) 198, 554
Bst2UI CCWGG 2 cut(s) 97, 596
Bst4CI ACNGT 2 cut(s) 476, 668
Bst6I CTCTTC 1 cut(s) 738
BstACI GRCGYC 1 cut(s) 161
BstAPI GCANNNNNTGC 1 cut(s) 521
BstBAI YACGTR 1 cut(s) 107
BstC8I GCNNGC 1 cut(s) 639
BstDEI CTNAG 3 cut(s) 151, 328, 753
BstDSI CCRYGG 1 cut(s) 198
BstEII GGTNACC 1 cut(s) 193
BstF5I GGATG 3 cut(s) 106, 403, 575
BstHHI GCGC 1 cut(s) 231
BstKTI GATC 1 cut(s) 794
BstMAI GTCTC 1 cut(s) 185
BstMBI GATC 1 cut(s) 791
BstMWI GCNNNNNNNGC 2 cut(s) 101, 521
BstNI CCWGG 2 cut(s) 97, 596
BstPAI GACNNNNGTC 1 cut(s) 343
BstPI GGTNACC 1 cut(s) 193
BstSCI CCNGG 2 cut(s) 95, 594
BstSLI GKGCMC 1 cut(s) 481
BstV1I GCAGC 1 cut(s) 449
BsuRI GGCC 1 cut(s) 611
BtgI CCRYGG 1 cut(s) 198
BtgZI GCGATG 1 cut(s) 792
BtsCI GGATG 3 cut(s) 106, 403, 575
BveI ACCTGC 1 cut(s) 38
Cac8I GCNNGC 1 cut(s) 639
CfoI GCGC 1 cut(s) 231
Csp6I GTAC 2 cut(s) 62, 559
CspCI CAANNNNNGTGG 2 cut(s) 552, 587
CviAII CATG 8 cut(s) 14, 166, 199, 506, 516, 720, 767, 787
CviQI GTAC 2 cut(s) 62, 559
DdeI CTNAG 3 cut(s) 151, 328, 753
DpnI GATC 1 cut(s) 793
DpnII GATC 1 cut(s) 791
DrdI GACNNNNNNGTC 1 cut(s) 166
DseDI GACNNNNNNGTC 1 cut(s) 166
EaeI YGGCCR 1 cut(s) 609
Eam1104I CTCTTC 1 cut(s) 738
EarI CTCTTC 1 cut(s) 738
Ecl136II GAGCTC 1 cut(s) 748
Eco130I CCWWGG 2 cut(s) 198, 554
Eco24I GRGCYC 1 cut(s) 750
Eco31I GGTCTC 1 cut(s) 185
Eco32I GATATC 1 cut(s) 428
Eco53kI GAGCTC 1 cut(s) 748
Eco88I CYCGRG 1 cut(s) 175
Eco91I GGTNACC 1 cut(s) 193
EcoICRI GAGCTC 1 cut(s) 748
EcoO65I GGTNACC 1 cut(s) 193
EcoRII CCWGG 2 cut(s) 95, 594
EcoRV GATATC 1 cut(s) 428
EcoT14I CCWWGG 2 cut(s) 198, 554
EcoT38I GRGCYC 1 cut(s) 750
ErhI CCWWGG 2 cut(s) 198, 554
FaeI CATG 8 cut(s) 17, 169, 202, 509, 519, 723, 770, 790
FaqI GGGAC 1 cut(s) 612
FatI CATG 8 cut(s) 13, 165, 198, 505, 515, 719, 766, 786
FauNDI CATATG 1 cut(s) 247
Fnu4HI GCNGC 2 cut(s) 438, 817
FokI GGATG 3 cut(s) 113, 390, 562
FriOI GRGCYC 1 cut(s) 750
Fsp4HI GCNGC 2 cut(s) 438, 817
FspBI CTAG 2 cut(s) 434, 555
FspI TGCGCA 1 cut(s) 230
GlaI GCGC 1 cut(s) 230
GluI GCNGC 2 cut(s) 438, 817
GsuI CTGGAG 1 cut(s) 510
HaeIII GGCC 1 cut(s) 611
HapII CCGG 1 cut(s) 397
HhaI GCGC 1 cut(s) 231
Hin1I GRCGYC 1 cut(s) 161
Hin1II CATG 8 cut(s) 17, 169, 202, 509, 519, 723, 770, 790
Hin6I GCGC 1 cut(s) 229
HinP1I GCGC 1 cut(s) 229
HincII GTYRAC 1 cut(s) 414
HindII GTYRAC 1 cut(s) 414
HinfI GANTC 4 cut(s) 149, 251, 298, 647
HpaII CCGG 1 cut(s) 397
HphI GGTGA 3 cut(s) 169, 454, 581
Hpy166II GTNNAC 2 cut(s) 414, 725
Hpy188I TCNGA 7 cut(s) 46, 154, 265, 376, 386, 408, 756
Hpy188III TCNNGA 2 cut(s) 146, 182
Hpy8I GTNNAC 2 cut(s) 414, 725
HpyAV CCTTC 2 cut(s) 491, 726
HpyCH4III ACNGT 2 cut(s) 476, 668
HpyCH4IV ACGT 2 cut(s) 106, 161
HpyCH4V TGCA 5 cut(s) 33, 104, 515, 617, 686
HpyF10VI GCNNNNNNNGC 2 cut(s) 101, 521
HpyF3I CTNAG 3 cut(s) 151, 328, 753
HpySE526I ACGT 2 cut(s) 106, 161
Hsp92I GRCGYC 1 cut(s) 161
Hsp92II CATG 8 cut(s) 17, 169, 202, 509, 519, 723, 770, 790
HspAI GCGC 1 cut(s) 229
KpnI GGTACC 1 cut(s) 562
Kzo9I GATC 1 cut(s) 791
LguI GCTCTTC 1 cut(s) 738
LmnI GCTCC 2 cut(s) 203, 753
LpnPI CCDG 9 cut(s) 43, 57, 82, 109, 410, 474, 581, 593, 608
Lsp1109I GCAGC 1 cut(s) 449
LweI GCATC 4 cut(s) 91, 584, 626, 769
MaeI CTAG 2 cut(s) 434, 555
MaeII ACGT 2 cut(s) 106, 161
MaeIII GTNAC 4 cut(s) 157, 168, 193, 734
MalI GATC 1 cut(s) 793
MboI GATC 1 cut(s) 791
MboII GAAGA 3 cut(s) 97, 435, 755
MhlI GDGCHC 2 cut(s) 481, 750
MluCI AATT 4 cut(s) 370, 450, 657, 771
MlyI GAGTC 1 cut(s) 143
MnlI CCTC 8 cut(s) 106, 109, 148, 245, 474, 493, 531, 762
MseI TTAA 1 cut(s) 209
MspA1I CMGCKG 3 cut(s) 71, 440, 797
MspI CCGG 1 cut(s) 397
MspR9I CCNGG 2 cut(s) 97, 596
Mva1269I GAATGC 1 cut(s) 526
MvaI CCWGG 2 cut(s) 97, 596
MwoI GCNNNNNNNGC 2 cut(s) 101, 521
NcoI CCATGG 1 cut(s) 198
NdeI CATATG 1 cut(s) 247
NdeII GATC 1 cut(s) 791
NlaIII CATG 8 cut(s) 17, 169, 202, 509, 519, 723, 770, 790
NlaIV GGNNCC 2 cut(s) 478, 560
NmuCI GTSAC 3 cut(s) 157, 168, 734
NsbI TGCGCA 1 cut(s) 230
PciSI GCTCTTC 1 cut(s) 738
PctI GAATGC 1 cut(s) 526
PfeI GAWTC 3 cut(s) 251, 298, 647
PkrI GCNGC 2 cut(s) 439, 818
PleI GAGTC 1 cut(s) 143
PpsI GAGTC 1 cut(s) 143
Ppu21I YACGTR 1 cut(s) 107
PshAI GACNNNNGTC 1 cut(s) 343
Psp124BI GAGCTC 1 cut(s) 750
Psp6I CCWGG 2 cut(s) 95, 594
PspEI GGTNACC 1 cut(s) 193
PspGI CCWGG 2 cut(s) 95, 594
PspN4I GGNNCC 2 cut(s) 478, 560
PvuII CAGCTG 1 cut(s) 797
RsaI GTAC 2 cut(s) 63, 560
RsaNI GTAC 2 cut(s) 62, 559
SacI GAGCTC 1 cut(s) 750
SapI GCTCTTC 1 cut(s) 738
SaqAI TTAA 1 cut(s) 209
SatI GCNGC 2 cut(s) 438, 817
Sau3AI GATC 1 cut(s) 791
ScaI AGTACT 1 cut(s) 63
SchI GAGTC 1 cut(s) 143
ScrFI CCNGG 2 cut(s) 97, 596
SduI GDGCHC 2 cut(s) 481, 750
SfaNI GCATC 4 cut(s) 91, 584, 626, 769
Sse9I AATT 4 cut(s) 370, 450, 657, 771
SsiI CCGC 4 cut(s) 69, 320, 440, 817
SspMI CTAG 2 cut(s) 434, 555
SstI GAGCTC 1 cut(s) 750
StyD4I CCNGG 2 cut(s) 95, 594
StyI CCWWGG 2 cut(s) 198, 554
TaaI ACNGT 2 cut(s) 476, 668
TaiI ACGT 2 cut(s) 109, 164
TaqI TCGA 1 cut(s) 183
TasI AATT 4 cut(s) 370, 450, 657, 771
TatI WGTACW 1 cut(s) 61
TauI GCSGC 1 cut(s) 819
TfiI GAWTC 3 cut(s) 251, 298, 647
Tru1I TTAA 1 cut(s) 209
Tru9I TTAA 1 cut(s) 209
TseFI GTSAC 3 cut(s) 157, 168, 734
TseI GCWGC 1 cut(s) 437
Tsp45I GTSAC 3 cut(s) 157, 168, 734
TspDTI ATGAA 5 cut(s) 63, 264, 462, 708, 783
TspGWI ACGGA 1 cut(s) 350
XmaJI CCTAGG 1 cut(s) 554
XspI CTAG 2 cut(s) 434, 555
ZraI GACGTC 1 cut(s) 162
ZrmI AGTACT 1 cut(s) 63
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.