FvH4_4g31690

Protein kinase 2B, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0017292
Physical Location & Seq
Reverse (-)
30733387 .. 30735281
1895 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g31690.t1

Sequence Viewer

Length: 1335 bp
ATGGGAAATTGTTTGAGGAAACATCGTCGAGAGCCAGACTCACCTGTTCTAACTCCTACTGGTGGTTCTTATCGATCCAAGAAAGTTAATGGAACAACAGTTGGAGACGGAACGATTCCAGCTTTTTCCTCAACTTCTATGGGAGAAAGGAAACGTGAAACACAGAGATCCCCACTTCAAGAAATCAAACATGTTACTTCAAACACAGATATAGGCGCAGAGAGGCATATTGATAAGAAAAGGCTTGCTCACAGAATTGCTGGAAAAGCTGCTAAGAAATCTAATATTGCGGTAGTGAATAAGAATGATGGGGTATTTCATGCTTACTACAAGTACAATGTGCACTGCTTTTGTTATAGTGACTTGAAAGAGGCGACACGCAAATTTAGCAGCAGAAATTTAATCGGGCAAGGGGGATTTGGTGATGTTTACATAGGGTATGTCACTTACTGCAGTATGGCCTCTGCTGAAAAACCAAATGAGGGGGTCGCAGTTGCCATCAAAAGGCTCAGAAAGACCGGGCCACAAGGACATGAACAATGGAAGAATGAAATCAAATACATGGGCAAACTTAACCATCCCAACATAGTGAAGCTAATAGGCTACTGCAAAGAGGGTGAGCAAAGGATATTGGTCTATGAGTACATGAGTAAAGGAAGCTTGGAGACCCAACTACTGGCAGAGAAGGCACCGCATTTAGATTGGAGAAGAAGAATTAACGTAGCAATTGATATAGCCAAGGCCCTAGATTCTCTTCACAATCATGGCACGCCAGTCATTCATCGAGATCTGAAAGCTTCCAATGTCTTGTTGGATAAAGACTTCAATGCTAAGCTATCAGATTTTGGCCTGGCAAGATATGGACCTCTAGCTGACCAGGACCGCATACTGACAAGGATTCTTGGCACCAAAGGCTATATAGCACCGGAATACATAGGAACTGGACATGTAACGCTGAAAACCGACGTGTACAGCTTTGGAGTGGTGCTGTTGGAGATTTTATCAGGCTGCTGTGCCGTGAAGAAATATTCCGATGGAATGGCCGGAGATCTAACACAGTGGGCTGGTCCATACCTCAGCAACAAGCAACAACTGCACCGCGTGATCGATCAGAGACTGGGAAGGAACTTCCCAACAAAAGAGGCTCAAAAGTTCGCGGAACTCACACTCCTATGCCTTGCTTCAGACCCCAAGTGCAGACCAACAATGACAGAAGTTGTGGCTGAGTTGAAGGAGTTGCAAGAAAATACAAGCAGCAGCGGTAGCAGTAGTAATCCGAAACCGTACTGTGCTCTTACATTTACCCCATGCCCTCCTCCTTGCATAGTACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

445

Amino Acids

49.34

Weight (kDa)

9.36

Isoelectric Point (pI)

36.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 131 - 408 5.8e-47 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 133 - 410 2.3e-51 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017292)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g31690
malus_domestica MD13G1076200.v1.1 MD16G1076400.v1.1
prunus_persica Prupe.1G270600_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0440201
rosa_laevigata RLG00000006196
rosa_multiflora Rmu_sc0001685.1_g000028
rosa_roxburghii Rroxscaffold_5G00381130
rosa_rugosa Rorug04G0325200 Rorug04G0325300.1
rosa_samantha Rh4AG376000 Rh4DG382800
rosa_wichuraiana Rw4G032180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 688, 905
AccB7I CCANNNNNTGG 1 cut(s) 676
AccII CGCG 2 cut(s) 1101, 1157
AciI CCGC 6 cut(s) 290, 692, 883, 1099, 1157, 1260
AclWI GGATC 2 cut(s) 69, 162
AcoI YGGCCR 1 cut(s) 1041
AcsI RAATTY 2 cut(s) 383, 397
AcuI CTGAAG 1 cut(s) 1167
AdeI CACNNNGTG 1 cut(s) 1102
AfaI GTAC 5 cut(s) 335, 644, 971, 1286, 1329
AfiI CCNNNNNNNGG 4 cut(s) 62, 482, 504, 676
AflIII ACRYGT 3 cut(s) 190, 946, 966
AgsI TTSAA 5 cut(s) 179, 201, 367, 826, 1231
AjiI CACGTC 1 cut(s) 967
AjnI CCWGG 2 cut(s) 849, 876
AloI GAACNNNNNNTCC 2 cut(s) 1152, 1184
AluBI AGCT 8 cut(s) 122, 269, 595, 660, 797, 835, 872, 975
AluI AGCT 8 cut(s) 122, 269, 595, 660, 797, 835, 872, 975
Alw21I GWGCWC 2 cut(s) 345, 1294
Alw26I GTCTC 3 cut(s) 99, 659, 1108
Alw44I GTGCAC 1 cut(s) 341
AlwI GGATC 2 cut(s) 69, 162
AlwNI CAGNNNCTG 1 cut(s) 1117
AoxI GGCC 5 cut(s) 459, 521, 741, 847, 1041
ApaLI GTGCAC 1 cut(s) 341
ApeKI GCWGC 5 cut(s) 269, 390, 1008, 1254, 1257
ApoI RAATTY 2 cut(s) 383, 397
AspLEI GCGC 1 cut(s) 218
AspS9I GGNCC 5 cut(s) 521, 742, 863, 880, 1067
AsuC2I CCSGG 1 cut(s) 520
AsuHPI GGTGA 3 cut(s) 33, 434, 629
AvaII GGWCC 3 cut(s) 863, 880, 1067
BaeGI GKGCMC 1 cut(s) 345
BanI GGYRCC 2 cut(s) 688, 905
Bbv12I GWGCWC 2 cut(s) 345, 1294
BbvCI CCTCAGC 1 cut(s) 1076
BbvI GCAGC 5 cut(s) 256, 402, 995, 1266, 1269
BccI CCATC 4 cut(s) 302, 506, 585, 1028
BceAI ACGGC 1 cut(s) 1001
BciT130I CCWGG 2 cut(s) 851, 878
BcnI CCSGG 1 cut(s) 520
BcoDI GTCTC 3 cut(s) 99, 659, 1108
BfaI CTAG 2 cut(s) 746, 869
BfmI CTRYAG 1 cut(s) 451
BglII AGATCT 2 cut(s) 787, 1048
BisI GCNGC 5 cut(s) 270, 391, 1009, 1255, 1258
BlpI GCTNAGC 1 cut(s) 831
BlsI GCNGC 5 cut(s) 271, 392, 1010, 1256, 1259
Bme1390I CCNGG 3 cut(s) 520, 851, 878
Bme18I GGWCC 3 cut(s) 863, 880, 1067
BmgBI CACGTC 1 cut(s) 967
BmgT120I GGNCC 5 cut(s) 521, 742, 863, 880, 1067
BmiI GGNNCC 2 cut(s) 690, 907
BmrFI CCNGG 3 cut(s) 520, 851, 878
BmrI ACTGGG 1 cut(s) 1127
BmuI ACTGGG 1 cut(s) 1127
BplI GAGNNNNNCTC 2 cut(s) 23, 55
Bpu10I CCTNAGC 1 cut(s) 1076
Bpu1102I GCTNAGC 1 cut(s) 831
BpuMI CCSGG 1 cut(s) 520
Bsa29I ATCGAT 2 cut(s) 73, 1107
BsaI GGTCTC 1 cut(s) 659
BsaJI CCNNGG 1 cut(s) 738
BsaWI WCCGGW 1 cut(s) 925
BsaXI ACNNNNNCTCC 2 cut(s) 1152, 1182
Bsc4I CCNNNNNNNGG 4 cut(s) 62, 482, 504, 676
Bse1I ACTGG 5 cut(s) 64, 681, 773, 946, 1122
BseBI CCWGG 2 cut(s) 851, 878
BseCI ATCGAT 2 cut(s) 73, 1107
BseDI CCNNGG 1 cut(s) 738
BseGI GGATG 1 cut(s) 577
BseLI CCNNNNNNNGG 4 cut(s) 62, 482, 504, 676
BseMII CTCAG 3 cut(s) 523, 1090, 1215
BseNI ACTGG 5 cut(s) 64, 681, 773, 946, 1122
BseRI GAGGAG 1 cut(s) 1305
BseSI GKGCMC 1 cut(s) 345
BseXI GCAGC 5 cut(s) 256, 402, 995, 1266, 1269
BsgI GTGCAG 2 cut(s) 1079, 1216
Bsh1236I CGCG 2 cut(s) 1101, 1157
BshFI GGCC 5 cut(s) 461, 523, 743, 849, 1043
BshNI GGYRCC 2 cut(s) 688, 905
BshVI ATCGAT 2 cut(s) 73, 1107
BsiHKAI GWGCWC 2 cut(s) 345, 1294
BsiSI CCGG 3 cut(s) 519, 926, 1044
BslI CCNNNNNNNGG 4 cut(s) 62, 482, 504, 676
BsmAI GTCTC 3 cut(s) 99, 659, 1108
BsmBI CGTCTC 1 cut(s) 99
BsnI GGCC 5 cut(s) 461, 523, 743, 849, 1043
Bso31I GGTCTC 1 cut(s) 659
Bsp1286I GDGCHC 2 cut(s) 345, 1294
Bsp1407I TGTACA 1 cut(s) 969
Bsp143I GATC 6 cut(s) 74, 167, 787, 1048, 1104, 1108
Bsp1720I GCTNAGC 1 cut(s) 831
BspACI CCGC 6 cut(s) 290, 692, 883, 1099, 1157, 1260
BspANI GGCC 5 cut(s) 461, 523, 743, 849, 1043
BspCNI CTCAG 3 cut(s) 522, 1089, 1216
BspDI ATCGAT 2 cut(s) 73, 1107
BspFNI CGCG 2 cut(s) 1101, 1157
BspLI GGNNCC 2 cut(s) 690, 907
BspMAI CTGCAG 1 cut(s) 455
BspPI GGATC 2 cut(s) 69, 162
BspT107I GGYRCC 2 cut(s) 688, 905
BspTNI GGTCTC 1 cut(s) 659
BsrGI TGTACA 1 cut(s) 969
BsrI ACTGG 5 cut(s) 64, 681, 773, 946, 1122
BssECI CCNNGG 1 cut(s) 738
BssMI GATC 6 cut(s) 74, 167, 787, 1048, 1104, 1108
BssT1I CCWWGG 1 cut(s) 738
Bst2UI CCWGG 2 cut(s) 851, 878
Bst4CI ACNGT 4 cut(s) 100, 1059, 1284, 1289
Bst6I CTCTTC 1 cut(s) 759
BstAPI GCANNNNNTGC 1 cut(s) 1093
BstAUI TGTACA 1 cut(s) 969
BstC8I GCNNGC 2 cut(s) 246, 770
BstDEI CTNAG 5 cut(s) 273, 509, 831, 1076, 1224
BstF5I GGATG 1 cut(s) 577
BstFNI CGCG 2 cut(s) 1101, 1157
BstHHI GCGC 1 cut(s) 218
BstKTI GATC 6 cut(s) 77, 170, 790, 1051, 1107, 1111
BstMAI GTCTC 3 cut(s) 99, 659, 1108
BstMBI GATC 6 cut(s) 74, 167, 787, 1048, 1104, 1108
BstMWI GCNNNNNNNGC 6 cut(s) 266, 387, 686, 912, 1093, 1263
BstNI CCWGG 2 cut(s) 851, 878
BstNSI RCATGY 2 cut(s) 194, 950
BstSCI CCNGG 3 cut(s) 518, 849, 876
BstSFI CTRYAG 1 cut(s) 451
BstSLI GKGCMC 1 cut(s) 345
BstUI CGCG 2 cut(s) 1101, 1157
BstV1I GCAGC 5 cut(s) 256, 402, 995, 1266, 1269
BstX2I RGATCY 3 cut(s) 167, 787, 1048
BstYI RGATCY 3 cut(s) 167, 787, 1048
Bsu15I ATCGAT 2 cut(s) 73, 1107
BsuRI GGCC 5 cut(s) 461, 523, 743, 849, 1043
BsuTUI ATCGAT 2 cut(s) 73, 1107
BtrI CACGTC 1 cut(s) 967
BtsCI GGATG 1 cut(s) 577
BtsI GCAGTG 1 cut(s) 343
BtsIMutI CAGTG 2 cut(s) 343, 1064
Cac8I GCNNGC 2 cut(s) 246, 770
CaiI CAGNNNCTG 1 cut(s) 1117
CfoI GCGC 1 cut(s) 218
Cfr13I GGNCC 5 cut(s) 521, 742, 863, 880, 1067
ClaI ATCGAT 2 cut(s) 73, 1107
Csp6I GTAC 5 cut(s) 334, 643, 970, 1285, 1328
CviAII CATG 8 cut(s) 191, 320, 533, 562, 646, 764, 947, 1308
CviQI GTAC 5 cut(s) 334, 643, 970, 1285, 1328
DdeI CTNAG 5 cut(s) 273, 509, 831, 1076, 1224
DpnI GATC 6 cut(s) 76, 169, 789, 1050, 1106, 1110
DpnII GATC 6 cut(s) 74, 167, 787, 1048, 1104, 1108
DraIII CACNNNGTG 1 cut(s) 1102
EaeI YGGCCR 1 cut(s) 1041
Eam1104I CTCTTC 1 cut(s) 759
EarI CTCTTC 1 cut(s) 759
Eco130I CCWWGG 1 cut(s) 738
Eco31I GGTCTC 1 cut(s) 659
Eco47I GGWCC 3 cut(s) 863, 880, 1067
Eco57I CTGAAG 1 cut(s) 1167
EcoO109I RGGNCCY 1 cut(s) 742
EcoRII CCWGG 2 cut(s) 849, 876
EcoT14I CCWWGG 1 cut(s) 738
ErhI CCWWGG 1 cut(s) 738
Esp3I CGTCTC 1 cut(s) 99
FaeI CATG 8 cut(s) 194, 323, 536, 565, 649, 767, 950, 1311
FatI CATG 8 cut(s) 190, 319, 532, 561, 645, 763, 946, 1307
Fnu4HI GCNGC 5 cut(s) 270, 391, 1009, 1255, 1258
FokI GGATG 1 cut(s) 564
Fsp4HI GCNGC 5 cut(s) 270, 391, 1009, 1255, 1258
FspBI CTAG 2 cut(s) 746, 869
GlaI GCGC 1 cut(s) 217
GluI GCNGC 5 cut(s) 270, 391, 1009, 1255, 1258
HaeIII GGCC 5 cut(s) 461, 523, 743, 849, 1043
HapII CCGG 3 cut(s) 519, 926, 1044
HhaI GCGC 1 cut(s) 218
Hin1II CATG 8 cut(s) 194, 323, 536, 565, 649, 767, 950, 1311
Hin6I GCGC 1 cut(s) 216
HinP1I GCGC 1 cut(s) 216
HindIII AAGCTT 2 cut(s) 658, 795
HinfI GANTC 4 cut(s) 38, 115, 749, 898
HpaII CCGG 3 cut(s) 519, 926, 1044
HphI GGTGA 3 cut(s) 33, 434, 629
Hpy166II GTNNAC 3 cut(s) 343, 430, 970
Hpy188I TCNGA 7 cut(s) 512, 792, 841, 1033, 1113, 1186, 1278
Hpy188III TCNNGA 3 cut(s) 29, 179, 785
Hpy8I GTNNAC 3 cut(s) 343, 430, 970
Hpy99I CGWCG 2 cut(s) 30, 968
HpyAV CCTTC 3 cut(s) 679, 1116, 1225
HpyCH4III ACNGT 4 cut(s) 100, 1059, 1284, 1289
HpyCH4IV ACGT 3 cut(s) 154, 720, 966
HpyCH4V TGCA 7 cut(s) 343, 453, 609, 1096, 1197, 1240, 1323
HpyF10VI GCNNNNNNNGC 6 cut(s) 266, 387, 686, 912, 1093, 1263
HpyF3I CTNAG 5 cut(s) 273, 509, 831, 1076, 1224
HpySE526I ACGT 3 cut(s) 154, 720, 966
Hsp92II CATG 8 cut(s) 194, 323, 536, 565, 649, 767, 950, 1311
HspAI GCGC 1 cut(s) 216
Kzo9I GATC 6 cut(s) 74, 167, 787, 1048, 1104, 1108
Lsp1109I GCAGC 5 cut(s) 256, 402, 995, 1266, 1269
MaeI CTAG 2 cut(s) 746, 869
MaeII ACGT 3 cut(s) 154, 720, 966
MaeIII GTNAC 4 cut(s) 193, 359, 442, 949
MalI GATC 6 cut(s) 76, 169, 789, 1050, 1106, 1110
MboI GATC 6 cut(s) 74, 167, 787, 1048, 1104, 1108
MboII GAAGA 5 cut(s) 556, 720, 723, 746, 1033
MfeI CAATTG 1 cut(s) 726
MflI RGATCY 3 cut(s) 167, 787, 1048
MhlI GDGCHC 2 cut(s) 345, 1294
MluCI AATT 6 cut(s) 7, 255, 383, 397, 714, 726
MlyI GAGTC 1 cut(s) 32
MmeI TCCRAC 3 cut(s) 82, 792, 972
MseI TTAA 4 cut(s) 87, 401, 573, 717
MslI CAYNNNNRTG 2 cut(s) 762, 1171
MspA1I CMGCKG 1 cut(s) 1260
MspI CCGG 3 cut(s) 519, 926, 1044
MspR9I CCNGG 3 cut(s) 520, 851, 878
MunI CAATTG 1 cut(s) 726
MvaI CCWGG 2 cut(s) 851, 878
MvnI CGCG 2 cut(s) 1101, 1157
MwoI GCNNNNNNNGC 6 cut(s) 266, 387, 686, 912, 1093, 1263
NciI CCSGG 1 cut(s) 520
NdeII GATC 6 cut(s) 74, 167, 787, 1048, 1104, 1108
NlaIII CATG 8 cut(s) 194, 323, 536, 565, 649, 767, 950, 1311
NlaIV GGNNCC 2 cut(s) 690, 907
NmuCI GTSAC 2 cut(s) 359, 442
NspI RCATGY 2 cut(s) 194, 950
PciI ACATGT 2 cut(s) 190, 946
PfeI GAWTC 3 cut(s) 115, 749, 898
PflMI CCANNNNNTGG 1 cut(s) 676
PkrI GCNGC 5 cut(s) 271, 392, 1010, 1256, 1259
PleI GAGTC 1 cut(s) 32
PpsI GAGTC 1 cut(s) 32
PscI ACATGT 2 cut(s) 190, 946
Psp6I CCWGG 2 cut(s) 849, 876
PspGI CCWGG 2 cut(s) 849, 876
PspN4I GGNNCC 2 cut(s) 690, 907
PspPI GGNCC 5 cut(s) 521, 742, 863, 880, 1067
PstI CTGCAG 1 cut(s) 455
PstNI CAGNNNCTG 1 cut(s) 1117
PsuI RGATCY 3 cut(s) 167, 787, 1048
RsaI GTAC 5 cut(s) 335, 644, 971, 1286, 1329
RsaNI GTAC 5 cut(s) 334, 643, 970, 1285, 1328
RseI CAYNNNNRTG 2 cut(s) 762, 1171
SaqAI TTAA 4 cut(s) 87, 401, 573, 717
SatI GCNGC 5 cut(s) 270, 391, 1009, 1255, 1258
Sau3AI GATC 6 cut(s) 74, 167, 787, 1048, 1104, 1108
Sau96I GGNCC 5 cut(s) 521, 742, 863, 880, 1067
SchI GAGTC 1 cut(s) 32
ScrFI CCNGG 3 cut(s) 520, 851, 878
SduI GDGCHC 2 cut(s) 345, 1294
SfcI CTRYAG 1 cut(s) 451
SinI GGWCC 3 cut(s) 863, 880, 1067
SmiMI CAYNNNNRTG 2 cut(s) 762, 1171
Sse9I AATT 6 cut(s) 7, 255, 383, 397, 714, 726
SsiI CCGC 6 cut(s) 290, 692, 883, 1099, 1157, 1260
SspI AATATT 2 cut(s) 286, 1028
SspMI CTAG 2 cut(s) 746, 869
StyD4I CCNGG 3 cut(s) 518, 849, 876
StyI CCWWGG 1 cut(s) 738
TaaI ACNGT 4 cut(s) 100, 1059, 1284, 1289
TaiI ACGT 3 cut(s) 157, 723, 969
TaqI TCGA 4 cut(s) 28, 73, 784, 1107
TasI AATT 6 cut(s) 7, 255, 383, 397, 714, 726
TatI WGTACW 4 cut(s) 333, 642, 969, 1327
TfiI GAWTC 3 cut(s) 115, 749, 898
Tru1I TTAA 4 cut(s) 87, 401, 573, 717
Tru9I TTAA 4 cut(s) 87, 401, 573, 717
TscAI CASTG 2 cut(s) 350, 1064
TseFI GTSAC 2 cut(s) 359, 442
TseI GCWGC 5 cut(s) 269, 390, 1008, 1254, 1257
Tsp45I GTSAC 2 cut(s) 359, 442
TspDTI ATGAA 4 cut(s) 308, 549, 564, 770
TspGWI ACGGA 1 cut(s) 123
TspRI CASTG 2 cut(s) 350, 1064
Van91I CCANNNNNTGG 1 cut(s) 676
VneI GTGCAC 1 cut(s) 341
VpaK11BI GGWCC 3 cut(s) 863, 880, 1067
XapI RAATTY 2 cut(s) 383, 397
XceI RCATGY 2 cut(s) 194, 950
XcmI CCANNNNNNNNNTGG 1 cut(s) 808
XspI CTAG 2 cut(s) 746, 869
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.