RchiOBHm_Chr4g0440201

Protein kinase 2B, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0017292
Physical Location & Seq
Reverse (-)
62439477 .. 62441128
1652 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40823

Sequence Viewer

Length: 1230 bp
ATGCATTCGGTTAATACAGAAACGAAGCGTGAAAAACAGAGATCCCCACTTCAAGAGATCACACATGTTGCTTCAAACACAGATATAGAAAAGCCTAGCAGAGAGAAAAGGCGTGGTTACCAAATTGCTCGAAAAGCTGGGAGTTCTAATATTGAGGGAGTGAACAAGAATGATGGAGTGTTTCATGCTTACTACAACTGCAAGCTACGCTGCTTTTGTTACAGTGACTTGAAAGTGGCCACGCGCAAATTTAGCAGCAGAAATTTAATCGGGCAAGGCGGATTTGGTGATGTATATAAAGGGTATGTGAGTTACTGCACTATGGACTCTGCTGCAAAACCAAATAAGGGACTCGCAATAGCCATCAAAAGGCTCAGAAAGACCGGGACACAAGGCCATGAACAATGGGAGAATGAAATCAAATTCATGTGCAAACTAAACCATCCCAACATAGTGAAGCTACTAGGCTACTGCCATGACGGTGGGCACAGGATGTTGGTTTATGAGTACATGAGCAAAGGAAGCTTGGAGACTCAACTACTGGCAGAGAATGCGCCGCAGTTAGATTGGAGCAGAAGAATCAATGTAGCAATTGGGTTAGCTAAGGCCCTAGATTCTCTTCACAATCATGGCACGCCGGTCATTCATAGAGATCTGAAAGCTTCCAATGTCCTGTTGGATGATGACTTCAATGCAAAGCTATCAGATTTTGGCCTGGCTAGATATGGACCTCTCTCTGACCAGGACCGCATAATGACAAGGATTCTTGGCACCAAAGGCTATATTGCACCGGAATACATAGGAACTGGACAGGTAACGCTGAAAACCGATGTGTACAGCTTTGGAGTGGTGCTGTTGGAGATTTTATCAGGCTGCTGTGCTGTGAAGAAATATTCAGATGGGATGGCCGGAGATCTTATACAGTGGGCTGGACCATATCTTTGCAACAAGCAACAACTGCATCAAGTGATTGACCAGAGACTGGGAAGGAACTTCCCAAAGAAAGAGGCTCAAAAGTTTGCGGAACTCATCCTCCACTGCCTTGCTTCAAACCCCAAGAGCAGACCAATAATGACTGAAGTTGTAGCTGAGTTGAAGGACTTACAAGAAAATACAAGCAGCAGCAGTAACAGCAGTACTCCGAACTCGTACCATACTCCTACATTTACCCCGTGCCCTCCTCCATACAAAATACTTTTACCTAGACGAAATGGATGTCAGACAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

409

Amino Acids

45.82

Weight (kDa)

9.28

Isoelectric Point (pI)

43.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 86 - 363 6.7e-47 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 88 - 365 5.5e-52 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0017292)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g31690
malus_domestica MD13G1076200.v1.1 MD16G1076400.v1.1
prunus_persica Prupe.1G270600_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0440201
rosa_laevigata RLG00000006196
rosa_multiflora Rmu_sc0001685.1_g000028
rosa_roxburghii Rroxscaffold_5G00381130
rosa_rugosa Rorug04G0325200 Rorug04G0325300.1
rosa_samantha Rh4AG376000 Rh4DG382800
rosa_wichuraiana Rw4G032180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 770
AccB7I CCANNNNNTGG 1 cut(s) 982
AccII CGCG 1 cut(s) 244
AciI CCGC 4 cut(s) 279, 557, 748, 1022
AclWI GGATC 1 cut(s) 36
AcoI YGGCCR 2 cut(s) 237, 906
AcsI RAATTY 3 cut(s) 248, 262, 422
AcuI CTGAAG 1 cut(s) 1098
AfaI GTAC 4 cut(s) 509, 836, 1138, 1151
AfiI CCNNNNNNNGG 3 cut(s) 347, 369, 982
AflIII ACRYGT 1 cut(s) 64
AgsI TTSAA 6 cut(s) 53, 75, 232, 691, 1050, 1096
AjnI CCWGG 2 cut(s) 714, 741
AloI GAACNNNNNNTCC 2 cut(s) 1017, 1049
AluBI AGCT 9 cut(s) 137, 205, 460, 525, 602, 662, 700, 840, 1088
AluI AGCT 9 cut(s) 137, 205, 460, 525, 602, 662, 700, 840, 1088
Alw26I GTCTC 2 cut(s) 524, 973
AlwI GGATC 1 cut(s) 36
AlwNI CAGNNNCTG 1 cut(s) 982
AoxI GGCC 5 cut(s) 237, 394, 606, 712, 906
ApeKI GCWGC 6 cut(s) 210, 255, 332, 873, 1119, 1122
ApoI RAATTY 3 cut(s) 248, 262, 422
ArsI GACNNNNNNTTYG 3 cut(s) 924, 956, 1201
AspLEI GCGC 2 cut(s) 246, 556
AspS9I GGNCC 4 cut(s) 607, 728, 745, 932
AsuC2I CCSGG 1 cut(s) 385
AsuHPI GGTGA 1 cut(s) 299
AvaII GGWCC 3 cut(s) 728, 745, 932
BaeGI GKGCMC 2 cut(s) 489, 1178
BalI TGGCCA 1 cut(s) 239
BanI GGYRCC 1 cut(s) 770
BbvI GCAGC 6 cut(s) 197, 267, 319, 860, 1131, 1134
BccI CCATC 5 cut(s) 167, 371, 450, 893, 898
BciT130I CCWGG 2 cut(s) 716, 743
BcnI CCSGG 1 cut(s) 385
BcoDI GTCTC 2 cut(s) 524, 973
BfaI CTAG 5 cut(s) 96, 464, 611, 720, 1203
BglII AGATCT 2 cut(s) 652, 913
BisI GCNGC 7 cut(s) 211, 256, 333, 557, 874, 1120, 1123
BlsI GCNGC 7 cut(s) 212, 257, 334, 558, 875, 1121, 1124
BmcAI AGTACT 1 cut(s) 1138
Bme1390I CCNGG 3 cut(s) 385, 716, 743
Bme18I GGWCC 3 cut(s) 728, 745, 932
BmgT120I GGNCC 4 cut(s) 607, 728, 745, 932
BmiI GGNNCC 1 cut(s) 772
BmrFI CCNGG 3 cut(s) 385, 716, 743
BmrI ACTGGG 1 cut(s) 992
BmsI GCATC 1 cut(s) 970
BmuI ACTGGG 1 cut(s) 992
Bpu10I CCTNAGC 1 cut(s) 603
BpuMI CCSGG 1 cut(s) 385
BsaWI WCCGGW 1 cut(s) 790
BsaXI ACNNNNNCTCC 2 cut(s) 1017, 1047
Bsc4I CCNNNNNNNGG 3 cut(s) 347, 369, 982
Bse118I RCCGGY 1 cut(s) 637
Bse1I ACTGG 3 cut(s) 546, 811, 987
BseBI CCWGG 2 cut(s) 716, 743
BseGI GGATG 6 cut(s) 442, 498, 685, 909, 1029, 1220
BseLI CCNNNNNNNGG 3 cut(s) 347, 369, 982
BseMII CTCAG 2 cut(s) 388, 1080
BseNI ACTGG 3 cut(s) 546, 811, 987
BseRI GAGGAG 1 cut(s) 1170
BseSI GKGCMC 2 cut(s) 489, 1178
BseXI GCAGC 6 cut(s) 197, 267, 319, 860, 1131, 1134
BseYI CCCAGC 1 cut(s) 137
BsgI GTGCAG 1 cut(s) 301
Bsh1236I CGCG 1 cut(s) 244
BshFI GGCC 5 cut(s) 239, 396, 608, 714, 908
BshNI GGYRCC 1 cut(s) 770
BsiSI CCGG 4 cut(s) 384, 638, 791, 909
BslFI GGGAC 2 cut(s) 363, 400
BslI CCNNNNNNNGG 3 cut(s) 347, 369, 982
BsmAI GTCTC 2 cut(s) 524, 973
BsmFI GGGAC 2 cut(s) 363, 400
BsmI GAATGC 2 cut(s) 4, 556
BsnI GGCC 5 cut(s) 239, 396, 608, 714, 908
Bsp1286I GDGCHC 2 cut(s) 489, 1178
Bsp1407I TGTACA 1 cut(s) 834
Bsp143I GATC 4 cut(s) 41, 57, 652, 913
BspACI CCGC 4 cut(s) 279, 557, 748, 1022
BspANI GGCC 5 cut(s) 239, 396, 608, 714, 908
BspCNI CTCAG 2 cut(s) 387, 1081
BspFNI CGCG 1 cut(s) 244
BspLI GGNNCC 1 cut(s) 772
BspPI GGATC 1 cut(s) 36
BspT107I GGYRCC 1 cut(s) 770
BsrFI RCCGGY 1 cut(s) 637
BsrGI TGTACA 1 cut(s) 834
BsrI ACTGG 3 cut(s) 546, 811, 987
BssAI RCCGGY 1 cut(s) 637
BssMI GATC 4 cut(s) 41, 57, 652, 913
Bst2UI CCWGG 2 cut(s) 716, 743
Bst4CI ACNGT 3 cut(s) 224, 482, 924
Bst6I CTCTTC 1 cut(s) 624
BstAPI GCANNNNNTGC 2 cut(s) 551, 958
BstAUI TGTACA 1 cut(s) 834
BstC8I GCNNGC 2 cut(s) 203, 635
BstDEI CTNAG 3 cut(s) 374, 603, 1089
BstEII GGTNACC 1 cut(s) 116
BstF5I GGATG 6 cut(s) 442, 498, 685, 909, 1029, 1220
BstFNI CGCG 1 cut(s) 244
BstHHI GCGC 2 cut(s) 246, 556
BstKTI GATC 4 cut(s) 44, 60, 655, 916
BstMAI GTCTC 2 cut(s) 524, 973
BstMBI GATC 4 cut(s) 41, 57, 652, 913
BstMWI GCNNNNNNNGC 8 cut(s) 134, 207, 252, 522, 551, 777, 958, 1131
BstNI CCWGG 2 cut(s) 716, 743
BstNSI RCATGY 1 cut(s) 68
BstPI GGTNACC 1 cut(s) 116
BstSCI CCNGG 3 cut(s) 383, 714, 741
BstSLI GKGCMC 2 cut(s) 489, 1178
BstUI CGCG 1 cut(s) 244
BstV1I GCAGC 6 cut(s) 197, 267, 319, 860, 1131, 1134
BstX2I RGATCY 3 cut(s) 41, 652, 913
BstXI CCANNNNNNTGG 1 cut(s) 482
BstYI RGATCY 3 cut(s) 41, 652, 913
BsuRI GGCC 5 cut(s) 239, 396, 608, 714, 908
BtsCI GGATG 6 cut(s) 442, 498, 685, 909, 1029, 1220
BtsI GCAGTG 1 cut(s) 1036
BtsIMutI CAGTG 3 cut(s) 229, 929, 1036
Cac8I GCNNGC 2 cut(s) 203, 635
CaiI CAGNNNCTG 1 cut(s) 982
CfoI GCGC 2 cut(s) 246, 556
Cfr10I RCCGGY 1 cut(s) 637
Cfr13I GGNCC 4 cut(s) 607, 728, 745, 932
Csp6I GTAC 4 cut(s) 508, 835, 1137, 1150
CviAII CATG 7 cut(s) 65, 185, 398, 427, 476, 511, 629
CviQI GTAC 4 cut(s) 508, 835, 1137, 1150
DdeI CTNAG 3 cut(s) 374, 603, 1089
DpnI GATC 4 cut(s) 43, 59, 654, 915
DpnII GATC 4 cut(s) 41, 57, 652, 913
EaeI YGGCCR 2 cut(s) 237, 906
Eam1104I CTCTTC 1 cut(s) 624
EarI CTCTTC 1 cut(s) 624
EciI GGCGGA 1 cut(s) 294
Eco47I GGWCC 3 cut(s) 728, 745, 932
Eco57I CTGAAG 1 cut(s) 1098
Eco91I GGTNACC 1 cut(s) 116
EcoO109I RGGNCCY 1 cut(s) 607
EcoO65I GGTNACC 1 cut(s) 116
EcoRII CCWGG 2 cut(s) 714, 741
EcoT22I ATGCAT 1 cut(s) 6
FaeI CATG 7 cut(s) 68, 188, 401, 430, 479, 514, 632
FaqI GGGAC 2 cut(s) 363, 400
FatI CATG 7 cut(s) 64, 184, 397, 426, 475, 510, 628
Fnu4HI GCNGC 7 cut(s) 211, 256, 333, 557, 874, 1120, 1123
FokI GGATG 5 cut(s) 429, 505, 692, 916, 1016
Fsp4HI GCNGC 7 cut(s) 211, 256, 333, 557, 874, 1120, 1123
FspBI CTAG 5 cut(s) 96, 464, 611, 720, 1203
GlaI GCGC 2 cut(s) 245, 555
GluI GCNGC 7 cut(s) 211, 256, 333, 557, 874, 1120, 1123
GsaI CCCAGC 1 cut(s) 141
HaeIII GGCC 5 cut(s) 239, 396, 608, 714, 908
HapII CCGG 4 cut(s) 384, 638, 791, 909
HhaI GCGC 2 cut(s) 246, 556
Hin1II CATG 7 cut(s) 68, 188, 401, 430, 479, 514, 632
Hin6I GCGC 2 cut(s) 244, 554
HinP1I GCGC 2 cut(s) 244, 554
HindIII AAGCTT 2 cut(s) 523, 660
HinfI GANTC 6 cut(s) 326, 351, 532, 579, 614, 763
HpaII CCGG 4 cut(s) 384, 638, 791, 909
HphI GGTGA 1 cut(s) 299
Hpy166II GTNNAC 2 cut(s) 163, 835
Hpy188I TCNGA 7 cut(s) 377, 657, 706, 739, 898, 1143, 1221
Hpy188III TCNNGA 1 cut(s) 53
Hpy8I GTNNAC 2 cut(s) 163, 835
HpyAV CCTTC 2 cut(s) 981, 1090
HpyCH4III ACNGT 3 cut(s) 224, 482, 924
HpyCH4V TGCA 9 cut(s) 4, 201, 318, 335, 432, 695, 788, 945, 961
HpyF10VI GCNNNNNNNGC 8 cut(s) 134, 207, 252, 522, 551, 777, 958, 1131
HpyF3I CTNAG 3 cut(s) 374, 603, 1089
Hsp92II CATG 7 cut(s) 68, 188, 401, 430, 479, 514, 632
HspAI GCGC 2 cut(s) 244, 554
Kzo9I GATC 4 cut(s) 41, 57, 652, 913
LmnI GCTCC 1 cut(s) 570
Lsp1109I GCAGC 6 cut(s) 197, 267, 319, 860, 1131, 1134
LweI GCATC 1 cut(s) 970
MaeI CTAG 5 cut(s) 96, 464, 611, 720, 1203
MaeIII GTNAC 6 cut(s) 116, 218, 224, 311, 814, 1127
MalI GATC 4 cut(s) 43, 59, 654, 915
MboI GATC 4 cut(s) 41, 57, 652, 913
MboII GAAGA 3 cut(s) 588, 611, 898
MfeI CAATTG 1 cut(s) 591
MflI RGATCY 3 cut(s) 41, 652, 913
MhlI GDGCHC 2 cut(s) 489, 1178
MlsI TGGCCA 1 cut(s) 239
MluCI AATT 5 cut(s) 123, 248, 262, 422, 591
MluNI TGGCCA 1 cut(s) 239
MlyI GAGTC 3 cut(s) 320, 345, 526
MmeI TCCRAC 2 cut(s) 657, 837
MnlI CCTC 6 cut(s) 148, 741, 1000, 1043, 1188, 1191
Mox20I TGGCCA 1 cut(s) 239
Mph1103I ATGCAT 1 cut(s) 6
MscI TGGCCA 1 cut(s) 239
MseI TTAA 2 cut(s) 12, 266
MslI CAYNNNNRTG 2 cut(s) 480, 627
Msp20I TGGCCA 1 cut(s) 239
MspI CCGG 4 cut(s) 384, 638, 791, 909
MspR9I CCNGG 3 cut(s) 385, 716, 743
MunI CAATTG 1 cut(s) 591
Mva1269I GAATGC 2 cut(s) 4, 556
MvaI CCWGG 2 cut(s) 716, 743
MvnI CGCG 1 cut(s) 244
MwoI GCNNNNNNNGC 8 cut(s) 134, 207, 252, 522, 551, 777, 958, 1131
NciI CCSGG 1 cut(s) 385
NdeII GATC 4 cut(s) 41, 57, 652, 913
NlaIII CATG 7 cut(s) 68, 188, 401, 430, 479, 514, 632
NlaIV GGNNCC 1 cut(s) 772
NmuCI GTSAC 1 cut(s) 224
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 68
PciI ACATGT 1 cut(s) 64
PctI GAATGC 2 cut(s) 4, 556
PfeI GAWTC 3 cut(s) 579, 614, 763
PflMI CCANNNNNTGG 1 cut(s) 982
PkrI GCNGC 7 cut(s) 212, 257, 334, 558, 875, 1121, 1124
PleI GAGTC 3 cut(s) 320, 345, 526
PpsI GAGTC 3 cut(s) 320, 345, 526
PscI ACATGT 1 cut(s) 64
Psp6I CCWGG 2 cut(s) 714, 741
PspEI GGTNACC 1 cut(s) 116
PspFI CCCAGC 1 cut(s) 137
PspGI CCWGG 2 cut(s) 714, 741
PspN4I GGNNCC 1 cut(s) 772
PspPI GGNCC 4 cut(s) 607, 728, 745, 932
PstNI CAGNNNCTG 1 cut(s) 982
PsuI RGATCY 3 cut(s) 41, 652, 913
RsaI GTAC 4 cut(s) 509, 836, 1138, 1151
RsaNI GTAC 4 cut(s) 508, 835, 1137, 1150
RseI CAYNNNNRTG 2 cut(s) 480, 627
SaqAI TTAA 2 cut(s) 12, 266
SatI GCNGC 7 cut(s) 211, 256, 333, 557, 874, 1120, 1123
Sau3AI GATC 4 cut(s) 41, 57, 652, 913
Sau96I GGNCC 4 cut(s) 607, 728, 745, 932
ScaI AGTACT 1 cut(s) 1138
SchI GAGTC 3 cut(s) 320, 345, 526
ScrFI CCNGG 3 cut(s) 385, 716, 743
SduI GDGCHC 2 cut(s) 489, 1178
SfaNI GCATC 1 cut(s) 970
SinI GGWCC 3 cut(s) 728, 745, 932
SmiMI CAYNNNNRTG 2 cut(s) 480, 627
Sse9I AATT 5 cut(s) 123, 248, 262, 422, 591
SsiI CCGC 4 cut(s) 279, 557, 748, 1022
SspI AATATT 2 cut(s) 151, 893
SspMI CTAG 5 cut(s) 96, 464, 611, 720, 1203
StyD4I CCNGG 3 cut(s) 383, 714, 741
TaaI ACNGT 3 cut(s) 224, 482, 924
TaqI TCGA 1 cut(s) 130
TasI AATT 5 cut(s) 123, 248, 262, 422, 591
TatI WGTACW 3 cut(s) 507, 834, 1136
TauI GCSGC 1 cut(s) 559
TfiI GAWTC 3 cut(s) 579, 614, 763
Tru1I TTAA 2 cut(s) 12, 266
Tru9I TTAA 2 cut(s) 12, 266
TscAI CASTG 3 cut(s) 229, 929, 1043
TseFI GTSAC 1 cut(s) 224
TseI GCWGC 6 cut(s) 210, 255, 332, 873, 1119, 1122
Tsp45I GTSAC 1 cut(s) 224
TspDTI ATGAA 5 cut(s) 173, 414, 415, 429, 635
TspRI CASTG 3 cut(s) 229, 929, 1043
Van91I CCANNNNNTGG 1 cut(s) 982
VpaK11BI GGWCC 3 cut(s) 728, 745, 932
XapI RAATTY 3 cut(s) 248, 262, 422
XceI RCATGY 1 cut(s) 68
XcmI CCANNNNNNNNNTGG 1 cut(s) 673
XspI CTAG 5 cut(s) 96, 464, 611, 720, 1203
ZrmI AGTACT 1 cut(s) 1138
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.