Rorug04G0325200

Protein kinase 2B, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0017292
Physical Location & Seq
Forward (+)
50017661 .. 50021513
3853 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0325200.1

Sequence Viewer

Length: 1074 bp
ATGTACGGATCCGATTCGGCTTCGGAGGATTTGGAGCGAACCGCTTTCCGAAAAGCCGAGAAGAAGTACAAGCTCTATTACCAAGACACTTACAGAACCCCCAAGAAGAAAAAGCAACCGAAGCCGGTGGATTTATCTGAGGTTTTGGATTTCAAGTCCATTCTAGAATCACATGATCAAAACGCTGAGCTTCCATCTGGAATCGCTCCAGTTCGGTGCGGTCTGGACCGTCCTGTTTTCTCCGTAGAGAATCGCCCAGGATTTTATTTCATTCCAGGAGCACTGACTATAGAACAACAATGTCAATGGATAAAGGAGAGTTTAACCAGCTTCCCACAGCCTCCTAACAGAACAAATCACAATGCTTTTTATGGGCCTATAAATGATTTATTTATTGCAGCAAAGGAGAGGAAAATTTTAGTTGCAAAAGAGAATTCAAATTCTGAATGTAATCCTTCTGTCAGCAATAGAGATGATCAAGGGTGGAAGTTCTTTGAGGAACATGAAACATCATCAAAAGGAAACTCATGCAAATCAATTTCAGCTTCAGTTCTGTTGCGGAAACTGCGTTGGAGCACCCTTGGCCTGCAATTTGAGTGGTCCAAGCGAAACTATGATGTTTCCCTTCCACATAAAAAGATCCCTGATACACTCTGTCAACTGGCTAAAAAACTGGCAGCACCTGCAATGCCCTCGAGTGAAGATTTCCAGCCTGAAGCTGCAATAGTGAACTACTTTGGCTTAGGTGATATGCTTGGGGGGCACCTGGATGACATGGAAGCTGATTGGAGTAAGCCTATTGTAAGCATGAGTTTAGGCTGCAAAGCAATATTCCTTCTGGGAGGAAAGCTTAGAGAGGATCCTCCATTAGCAATGTTCCTTCGAAGTGGTGATGTTGTACTTATGGCTGGAGAAGCAAGAGAATGCTTTCATGGCGTGCCTCGGATATTCACAGACAGAGAAAATGCTGAAATTGTGCCTCTTGAGCAGCAGTTCTCACACGAGGATGATTCAAGTTTTTTAGAATACATCCAAACTTCAAGAATCAACATCAACATTAGACAAGTATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

357

Amino Acids

40.49

Weight (kDa)

6.05

Isoelectric Point (pI)

49.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy_2 PF13532 167 - 354 4.3e-38 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017292)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g31690
malus_domestica MD13G1076200.v1.1 MD16G1076400.v1.1
prunus_persica Prupe.1G270600_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0440201
rosa_laevigata RLG00000006196
rosa_multiflora Rmu_sc0001685.1_g000028
rosa_roxburghii Rroxscaffold_5G00381130
rosa_rugosa Rorug04G0325200 Rorug04G0325300.1
rosa_samantha Rh4AG376000 Rh4DG382800
rosa_wichuraiana Rw4G032180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 691
Acc36I ACCTGC 1 cut(s) 691
AccB1I GGYRCC 1 cut(s) 762
AciI CCGC 3 cut(s) 42, 219, 559
AclWI GGATC 5 cut(s) 3, 16, 634, 854, 867
AcsI RAATTY 3 cut(s) 414, 433, 439
AcuI CTGAAG 2 cut(s) 531, 735
AfaI GTAC 3 cut(s) 5, 68, 900
AgsI TTSAA 4 cut(s) 154, 438, 1014, 1041
AjnI CCWGG 3 cut(s) 256, 274, 765
AluBI AGCT 7 cut(s) 73, 190, 330, 545, 719, 782, 850
AluI AGCT 7 cut(s) 73, 190, 330, 545, 719, 782, 850
Alw21I GWGCWC 2 cut(s) 283, 578
AlwI GGATC 5 cut(s) 3, 16, 634, 854, 867
AlwNI CAGNNNCTG 1 cut(s) 683
Ama87I CYCGRG 1 cut(s) 694
AoxI GGCC 2 cut(s) 374, 583
ApeKI GCWGC 5 cut(s) 398, 677, 719, 819, 988
ApoI RAATTY 3 cut(s) 414, 433, 439
Asp700I GAANNNNTTC 1 cut(s) 927
AspS9I GGNCC 3 cut(s) 226, 374, 600
AsuHPI GGTGA 2 cut(s) 758, 902
AsuII TTCGAA 1 cut(s) 883
AvaI CYCGRG 1 cut(s) 694
AvaII GGWCC 2 cut(s) 226, 600
BaeGI GKGCMC 1 cut(s) 765
BamHI GGATCC 2 cut(s) 8, 859
BanI GGYRCC 1 cut(s) 762
BauI CACGAG 1 cut(s) 1001
Bbv12I GWGCWC 2 cut(s) 283, 578
BbvI GCAGC 5 cut(s) 410, 689, 706, 806, 1000
BccI CCATC 1 cut(s) 202
BcgI CGANNNNNNTGC 2 cut(s) 675, 709
BciT130I CCWGG 3 cut(s) 258, 276, 767
BclI TGATCA 2 cut(s) 175, 475
BfaI CTAG 1 cut(s) 164
BfmI CTRYAG 1 cut(s) 288
BfuAI ACCTGC 1 cut(s) 691
BisI GCNGC 5 cut(s) 399, 678, 720, 820, 989
BlpI GCTNAGC 1 cut(s) 186
BlsI GCNGC 5 cut(s) 400, 679, 721, 821, 990
Bme1390I CCNGG 3 cut(s) 258, 276, 767
Bme18I GGWCC 2 cut(s) 226, 600
BmeT110I CYCGRG 1 cut(s) 694
BmgT120I GGNCC 3 cut(s) 226, 374, 600
BmiI GGNNCC 3 cut(s) 10, 764, 861
BmrFI CCNGG 3 cut(s) 258, 276, 767
BpmI CTGGAG 2 cut(s) 192, 930
Bpu10I CCTNAGC 1 cut(s) 742
Bpu1102I GCTNAGC 1 cut(s) 186
Bpu14I TTCGAA 1 cut(s) 883
BpuEI CTTGAG 1 cut(s) 1004
BsaJI CCNNGG 3 cut(s) 256, 580, 941
Bse118I RCCGGY 1 cut(s) 124
Bse1I ACTGG 3 cut(s) 209, 666, 678
Bse3DI GCAATG 2 cut(s) 693, 879
BseBI CCWGG 3 cut(s) 258, 276, 767
BseDI CCNNGG 3 cut(s) 256, 580, 941
BseGI GGATG 3 cut(s) 775, 1012, 1029
BseMI GCAATG 2 cut(s) 693, 879
BseMII CTCAG 2 cut(s) 129, 177
BseNI ACTGG 3 cut(s) 209, 666, 678
BseSI GKGCMC 1 cut(s) 765
BseXI GCAGC 5 cut(s) 410, 689, 706, 806, 1000
BshFI GGCC 2 cut(s) 376, 585
BshNI GGYRCC 1 cut(s) 762
BsiHKAI GWGCWC 2 cut(s) 283, 578
BsiHKCI CYCGRG 1 cut(s) 694
BsiSI CCGG 1 cut(s) 125
BsmI GAATGC 1 cut(s) 929
BsnI GGCC 2 cut(s) 376, 585
BsoBI CYCGRG 1 cut(s) 694
Bsp119I TTCGAA 1 cut(s) 883
Bsp1286I GDGCHC 3 cut(s) 283, 578, 765
Bsp143I GATC 5 cut(s) 8, 175, 475, 639, 859
Bsp1720I GCTNAGC 1 cut(s) 186
BspACI CCGC 3 cut(s) 42, 219, 559
BspANI GGCC 2 cut(s) 376, 585
BspCNI CTCAG 2 cut(s) 130, 178
BspLI GGNNCC 3 cut(s) 10, 764, 861
BspMI ACCTGC 1 cut(s) 691
BspPI GGATC 5 cut(s) 3, 16, 634, 854, 867
BspT104I TTCGAA 1 cut(s) 883
BspT107I GGYRCC 1 cut(s) 762
BsrDI GCAATG 2 cut(s) 693, 879
BsrFI RCCGGY 1 cut(s) 124
BsrI ACTGG 3 cut(s) 209, 666, 678
BssAI RCCGGY 1 cut(s) 124
BssECI CCNNGG 3 cut(s) 256, 580, 941
BssMI GATC 5 cut(s) 8, 175, 475, 639, 859
BssSI CACGAG 1 cut(s) 1001
BssT1I CCWWGG 1 cut(s) 580
Bst2BI CACGAG 1 cut(s) 1001
Bst2UI CCWGG 3 cut(s) 258, 276, 767
Bst4CI ACNGT 1 cut(s) 230
BstAPI GCANNNNNTGC 1 cut(s) 683
BstBI TTCGAA 1 cut(s) 883
BstC8I GCNNGC 2 cut(s) 587, 938
BstDEI CTNAG 4 cut(s) 138, 186, 742, 851
BstF5I GGATG 3 cut(s) 775, 1012, 1029
BstKTI GATC 5 cut(s) 11, 178, 478, 642, 862
BstMBI GATC 5 cut(s) 8, 175, 475, 639, 859
BstMWI GCNNNNNNNGC 8 cut(s) 121, 565, 582, 683, 760, 914, 933, 985
BstNI CCWGG 3 cut(s) 258, 276, 767
BstSCI CCNGG 3 cut(s) 256, 274, 765
BstSFI CTRYAG 1 cut(s) 288
BstSLI GKGCMC 1 cut(s) 765
BstV1I GCAGC 5 cut(s) 410, 689, 706, 806, 1000
BstX2I RGATCY 3 cut(s) 8, 639, 859
BstYI RGATCY 3 cut(s) 8, 639, 859
BsuRI GGCC 2 cut(s) 376, 585
BtsCI GGATG 3 cut(s) 775, 1012, 1029
BtsIMutI CAGTG 1 cut(s) 281
BveI ACCTGC 1 cut(s) 691
Cac8I GCNNGC 2 cut(s) 587, 938
CaiI CAGNNNCTG 1 cut(s) 683
Cfr10I RCCGGY 1 cut(s) 124
Cfr13I GGNCC 3 cut(s) 226, 374, 600
Csp6I GTAC 3 cut(s) 4, 67, 899
CspCI CAANNNNNGTGG 2 cut(s) 578, 613
CviAII CATG 6 cut(s) 173, 503, 528, 775, 808, 932
CviQI GTAC 3 cut(s) 4, 67, 899
DdeI CTNAG 4 cut(s) 138, 186, 742, 851
DpnI GATC 5 cut(s) 10, 177, 477, 641, 861
DpnII GATC 5 cut(s) 8, 175, 475, 639, 859
Eco130I CCWWGG 1 cut(s) 580
Eco47I GGWCC 2 cut(s) 226, 600
Eco57I CTGAAG 2 cut(s) 531, 735
Eco88I CYCGRG 1 cut(s) 694
EcoRI GAATTC 1 cut(s) 433
EcoRII CCWGG 3 cut(s) 256, 274, 765
EcoT14I CCWWGG 1 cut(s) 580
ErhI CCWWGG 1 cut(s) 580
FaeI CATG 6 cut(s) 176, 506, 531, 778, 811, 935
FatI CATG 6 cut(s) 172, 502, 527, 774, 807, 931
FbaI TGATCA 2 cut(s) 175, 475
Fnu4HI GCNGC 5 cut(s) 399, 678, 720, 820, 989
FokI GGATG 3 cut(s) 782, 1016, 1019
Fsp4HI GCNGC 5 cut(s) 399, 678, 720, 820, 989
FspBI CTAG 1 cut(s) 164
GluI GCNGC 5 cut(s) 399, 678, 720, 820, 989
GsuI CTGGAG 2 cut(s) 192, 930
HaeIII GGCC 2 cut(s) 376, 585
HapII CCGG 1 cut(s) 125
Hin1II CATG 6 cut(s) 176, 506, 531, 778, 811, 935
HincII GTYRAC 1 cut(s) 659
HindII GTYRAC 1 cut(s) 659
HindIII AAGCTT 1 cut(s) 848
HinfI GANTC 6 cut(s) 14, 167, 201, 250, 1010, 1044
HpaII CCGG 1 cut(s) 125
HphI GGTGA 2 cut(s) 758, 902
Hpy166II GTNNAC 2 cut(s) 659, 730
Hpy188I TCNGA 7 cut(s) 13, 25, 50, 139, 445, 945, 1073
Hpy188III TCNNGA 5 cut(s) 164, 198, 224, 983, 1041
Hpy8I GTNNAC 2 cut(s) 659, 730
HpyAV CCTTC 4 cut(s) 465, 635, 845, 890
HpyCH4III ACNGT 1 cut(s) 230
HpyCH4V TGCA 7 cut(s) 398, 425, 531, 589, 686, 722, 822
HpyF10VI GCNNNNNNNGC 8 cut(s) 121, 565, 582, 683, 760, 914, 933, 985
HpyF3I CTNAG 4 cut(s) 138, 186, 742, 851
Hsp92II CATG 6 cut(s) 176, 506, 531, 778, 811, 935
Ksp22I TGATCA 2 cut(s) 175, 475
Kzo9I GATC 5 cut(s) 8, 175, 475, 639, 859
LmnI GCTCC 4 cut(s) 34, 211, 278, 573
Lsp1109I GCAGC 5 cut(s) 410, 689, 706, 806, 1000
MaeI CTAG 1 cut(s) 164
MalI GATC 5 cut(s) 10, 177, 477, 641, 861
MboI GATC 5 cut(s) 8, 175, 475, 639, 859
MboII GAAGA 3 cut(s) 73, 118, 713
MflI RGATCY 3 cut(s) 8, 639, 859
MhlI GDGCHC 3 cut(s) 283, 578, 765
MluCI AATT 6 cut(s) 414, 433, 439, 537, 590, 972
MmeI TCCRAC 1 cut(s) 551
MroXI GAANNNNTTC 1 cut(s) 927
MseI TTAA 1 cut(s) 323
MslI CAYNNNNRTG 2 cut(s) 768, 1005
MspI CCGG 1 cut(s) 125
MspR9I CCNGG 3 cut(s) 258, 276, 767
Mva1269I GAATGC 1 cut(s) 929
MvaI CCWGG 3 cut(s) 258, 276, 767
MwoI GCNNNNNNNGC 8 cut(s) 121, 565, 582, 683, 760, 914, 933, 985
NdeII GATC 5 cut(s) 8, 175, 475, 639, 859
NlaIII CATG 6 cut(s) 176, 506, 531, 778, 811, 935
NlaIV GGNNCC 3 cut(s) 10, 764, 861
NmeAIII GCCGAG 1 cut(s) 82
NspV TTCGAA 1 cut(s) 883
PaeR7I CTCGAG 1 cut(s) 694
PaqCI CACCTGC 1 cut(s) 691
PctI GAATGC 1 cut(s) 929
PdmI GAANNNNTTC 1 cut(s) 927
PfeI GAWTC 6 cut(s) 14, 167, 201, 250, 1010, 1044
PfoI TCCNGGA 1 cut(s) 274
PkrI GCNGC 5 cut(s) 400, 679, 721, 821, 990
Psp6I CCWGG 3 cut(s) 256, 274, 765
PspGI CCWGG 3 cut(s) 256, 274, 765
PspN4I GGNNCC 3 cut(s) 10, 764, 861
PspPI GGNCC 3 cut(s) 226, 374, 600
PspXI VCTCGAGB 1 cut(s) 694
PstNI CAGNNNCTG 1 cut(s) 683
PsuI RGATCY 3 cut(s) 8, 639, 859
RsaI GTAC 3 cut(s) 5, 68, 900
RsaNI GTAC 3 cut(s) 4, 67, 899
RseI CAYNNNNRTG 2 cut(s) 768, 1005
SaqAI TTAA 1 cut(s) 323
SatI GCNGC 5 cut(s) 399, 678, 720, 820, 989
Sau3AI GATC 5 cut(s) 8, 175, 475, 639, 859
Sau96I GGNCC 3 cut(s) 226, 374, 600
ScrFI CCNGG 3 cut(s) 258, 276, 767
SduI GDGCHC 3 cut(s) 283, 578, 765
SfcI CTRYAG 1 cut(s) 288
Sfr274I CTCGAG 1 cut(s) 694
SfuI TTCGAA 1 cut(s) 883
SinI GGWCC 2 cut(s) 226, 600
SlaI CTCGAG 1 cut(s) 694
SmiMI CAYNNNNRTG 2 cut(s) 768, 1005
SmlI CTYRAG 2 cut(s) 694, 983
SmoI CTYRAG 2 cut(s) 694, 983
Sse9I AATT 6 cut(s) 414, 433, 439, 537, 590, 972
SsiI CCGC 3 cut(s) 42, 219, 559
SspI AATATT 1 cut(s) 831
SspMI CTAG 1 cut(s) 164
StyD4I CCNGG 3 cut(s) 256, 274, 765
StyI CCWWGG 1 cut(s) 580
TaaI ACNGT 1 cut(s) 230
TaqI TCGA 2 cut(s) 695, 883
TasI AATT 6 cut(s) 414, 433, 439, 537, 590, 972
TatI WGTACW 2 cut(s) 66, 898
TfiI GAWTC 6 cut(s) 14, 167, 201, 250, 1010, 1044
Tru1I TTAA 1 cut(s) 323
Tru9I TTAA 1 cut(s) 323
TscAI CASTG 1 cut(s) 288
TseI GCWGC 5 cut(s) 398, 677, 719, 819, 988
TspDTI ATGAA 3 cut(s) 259, 519, 920
TspGWI ACGGA 2 cut(s) 21, 232
TspRI CASTG 1 cut(s) 288
VpaK11BI GGWCC 2 cut(s) 226, 600
XapI RAATTY 3 cut(s) 414, 433, 439
XbaI TCTAGA 1 cut(s) 163
XhoI CTCGAG 1 cut(s) 694
XmnI GAANNNNTTC 1 cut(s) 927
XspI CTAG 1 cut(s) 164
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.