Rh4AG376000

Protein kinase 2B, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0017292
Physical Location & Seq
Reverse (-)
68312693 .. 68316011
3319 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG376000.1

Sequence Viewer

Length: 1368 bp
ATGGGAAATTGTTGCAGAAAACATCTTCAAGAGCCAAACTCACCGGTTCACGTTATTTCTCAAACTCCTACTGGCGGTTCTCATCGATCCAAGAAAGTTAATAGAACAACAGTTGGAGACAGAACTACTTCAACTGTTTCCTCAACTTCTGTGGGAGAAACGAAGCGTGAAAAACAGAGATCCCCACTTCAAGAGATCACACATGTTGCTTCAAACACAGATATAGAAAAGCCTAGCAGAGAGAAAAGGCGTGGTTACCAAATTGCTCGAAAAGCTGGGAGTTCTAATATTGAGGGAGTGAACAAGAATGATGGAGTGTTTCATGCTTACTACAACTGCAAGCTACGCTGCTTTTGTTACAGTGACTTGAAAGTGGCCACGCGCAAATTTAGCAGCAGAAATTTAATCGGGCAAGGCGGATTTGGTGATGTATATAAAGGGTATGTGAGTTACTGCACTATGGACTCTGCTGCAAAACCAAATAAGGGACTCGCAATAGCCATCAAAAGGCTCAGAAAGACCGGGACACAAGGCCATGAACAATGGGAGAATGAAATCAAATTCATGTGCAAACTAAACCATCCCAACATAGTGAAGCTACTAGGCTACTGCCATGACGGTGGGCACAGGATGTTGGTTTATGAGTACATGAGCAAAGGAAGCTTGGAGACTCAACTACTGGCAGAGAATGCGCCGCAGTTAGATTGGAGCAGAAGAATCAATGTAGCAATTGGGTTAGCTAAGGCCCTAGATTCTCTTCACAATCATGGCACGCCGGTCATTCATAGAGATCTGAAAGCTTCCAATGTCCTGTTGGATGATGACTTCAATGCAAAGCTATCAGATTTTGGCCTGGCTAGATATGGACCTCTCTCTGACCAGGACCGCATAATGACAAGGATTCTTGGCACCAAAGGCTATATTGCACCGGAATACATAGGAACTGGACAGGTAACGCTGAAAACCGATGTGTACAGCTTTGGAGTGGTGCTGTTGGAGATTTTATCAGGCTGCTGTGCTGTGAAGAAATATTCAGATGGGATGGCCGGAGATCTTATACAGTGGGCTGGACCATATCTTTGCAACAAGCAACAACTGCATCAAGTGATTGACCAGAGACTGGGAAGGAACTTCCCAAAGAAAGAGGCTCAAAAGTTTGCGGAACTCATCCTCCACTGCCTTGCTTCAAACCCCAAGAGCAGACCAATAATGACTGAAGTTGTAGCTGAGTTGAAGGACTTACAAGAAAATACAAGCAGCAGCAGTAACAGCAGTACTCCGAACTCGTACCATACTCCTACATTTACCCCGTGCCCTCCTCCATACAAAATACTTTTACCTAGACGAAATGGATGTCAGACAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

455

Amino Acids

50.66

Weight (kDa)

9.4

Isoelectric Point (pI)

42.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 132 - 409 9.2e-47 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 134 - 411 7.6e-52 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0017292)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g31690
malus_domestica MD13G1076200.v1.1 MD16G1076400.v1.1
prunus_persica Prupe.1G270600_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0440201
rosa_laevigata RLG00000006196
rosa_multiflora Rmu_sc0001685.1_g000028
rosa_roxburghii Rroxscaffold_5G00381130
rosa_rugosa Rorug04G0325200 Rorug04G0325300.1
rosa_samantha Rh4AG376000 Rh4DG382800
rosa_wichuraiana Rw4G032180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 908
AccB7I CCANNNNNTGG 1 cut(s) 1120
AccII CGCG 1 cut(s) 382
AciI CCGC 5 cut(s) 75, 417, 695, 886, 1160
AclWI GGATC 2 cut(s) 81, 174
AcoI YGGCCR 2 cut(s) 375, 1044
AcsI RAATTY 3 cut(s) 386, 400, 560
AcuI CTGAAG 1 cut(s) 1236
AfaI GTAC 4 cut(s) 647, 974, 1276, 1289
AfiI CCNNNNNNNGG 4 cut(s) 74, 485, 507, 1120
AflIII ACRYGT 1 cut(s) 202
AgeI ACCGGT 1 cut(s) 43
AgsI TTSAA 8 cut(s) 29, 132, 191, 213, 370, 829, 1188, 1234
AjnI CCWGG 2 cut(s) 852, 879
AloI GAACNNNNNNTCC 2 cut(s) 1155, 1187
AluBI AGCT 9 cut(s) 275, 343, 598, 663, 740, 800, 838, 978, 1226
AluI AGCT 9 cut(s) 275, 343, 598, 663, 740, 800, 838, 978, 1226
Alw26I GTCTC 3 cut(s) 111, 662, 1111
AlwI GGATC 2 cut(s) 81, 174
AlwNI CAGNNNCTG 1 cut(s) 1120
AoxI GGCC 5 cut(s) 375, 532, 744, 850, 1044
ApeKI GCWGC 6 cut(s) 348, 393, 470, 1011, 1257, 1260
ApoI RAATTY 3 cut(s) 386, 400, 560
ArsI GACNNNNNNTTYG 3 cut(s) 1062, 1094, 1339
AsiGI ACCGGT 1 cut(s) 43
Asp700I GAANNNNTTC 1 cut(s) 127
AspLEI GCGC 2 cut(s) 384, 694
AspS9I GGNCC 4 cut(s) 745, 866, 883, 1070
AsuC2I CCSGG 1 cut(s) 523
AsuHPI GGTGA 2 cut(s) 33, 437
AvaII GGWCC 3 cut(s) 866, 883, 1070
BaeGI GKGCMC 2 cut(s) 627, 1316
BalI TGGCCA 1 cut(s) 377
BanI GGYRCC 1 cut(s) 908
BbvI GCAGC 6 cut(s) 335, 405, 457, 998, 1269, 1272
BccI CCATC 5 cut(s) 305, 509, 588, 1031, 1036
BciT130I CCWGG 2 cut(s) 854, 881
BcnI CCSGG 1 cut(s) 523
BcoDI GTCTC 3 cut(s) 111, 662, 1111
BfaI CTAG 5 cut(s) 234, 602, 749, 858, 1341
BglII AGATCT 2 cut(s) 790, 1051
BisI GCNGC 7 cut(s) 349, 394, 471, 695, 1012, 1258, 1261
BlsI GCNGC 7 cut(s) 350, 395, 472, 696, 1013, 1259, 1262
BmcAI AGTACT 1 cut(s) 1276
Bme1390I CCNGG 3 cut(s) 523, 854, 881
Bme18I GGWCC 3 cut(s) 866, 883, 1070
BmgT120I GGNCC 4 cut(s) 745, 866, 883, 1070
BmiI GGNNCC 1 cut(s) 910
BmrFI CCNGG 3 cut(s) 523, 854, 881
BmrI ACTGGG 1 cut(s) 1130
BmsI GCATC 1 cut(s) 1108
BmuI ACTGGG 1 cut(s) 1130
BplI GAGNNNNNCTC 2 cut(s) 23, 55
Bpu10I CCTNAGC 1 cut(s) 741
BpuMI CCSGG 1 cut(s) 523
Bsa29I ATCGAT 1 cut(s) 85
BsaWI WCCGGW 2 cut(s) 43, 928
BsaXI ACNNNNNCTCC 2 cut(s) 1155, 1185
Bsc4I CCNNNNNNNGG 4 cut(s) 74, 485, 507, 1120
Bse118I RCCGGY 2 cut(s) 43, 775
Bse1I ACTGG 4 cut(s) 76, 684, 949, 1125
BseBI CCWGG 2 cut(s) 854, 881
BseCI ATCGAT 1 cut(s) 85
BseGI GGATG 6 cut(s) 580, 636, 823, 1047, 1167, 1358
BseLI CCNNNNNNNGG 4 cut(s) 74, 485, 507, 1120
BseMII CTCAG 2 cut(s) 526, 1218
BseNI ACTGG 4 cut(s) 76, 684, 949, 1125
BseRI GAGGAG 1 cut(s) 1308
BseSI GKGCMC 2 cut(s) 627, 1316
BseXI GCAGC 6 cut(s) 335, 405, 457, 998, 1269, 1272
BseYI CCCAGC 1 cut(s) 275
BsgI GTGCAG 1 cut(s) 439
Bsh1236I CGCG 1 cut(s) 382
BshFI GGCC 5 cut(s) 377, 534, 746, 852, 1046
BshNI GGYRCC 1 cut(s) 908
BshTI ACCGGT 1 cut(s) 43
BshVI ATCGAT 1 cut(s) 85
BsiSI CCGG 5 cut(s) 44, 522, 776, 929, 1047
BslFI GGGAC 2 cut(s) 501, 538
BslI CCNNNNNNNGG 4 cut(s) 74, 485, 507, 1120
BsmAI GTCTC 3 cut(s) 111, 662, 1111
BsmFI GGGAC 2 cut(s) 501, 538
BsmI GAATGC 1 cut(s) 694
BsnI GGCC 5 cut(s) 377, 534, 746, 852, 1046
Bsp1286I GDGCHC 2 cut(s) 627, 1316
Bsp1407I TGTACA 1 cut(s) 972
Bsp143I GATC 5 cut(s) 86, 179, 195, 790, 1051
BspACI CCGC 5 cut(s) 75, 417, 695, 886, 1160
BspANI GGCC 5 cut(s) 377, 534, 746, 852, 1046
BspCNI CTCAG 2 cut(s) 525, 1219
BspDI ATCGAT 1 cut(s) 85
BspFNI CGCG 1 cut(s) 382
BspLI GGNNCC 1 cut(s) 910
BspPI GGATC 2 cut(s) 81, 174
BspT107I GGYRCC 1 cut(s) 908
BsrFI RCCGGY 2 cut(s) 43, 775
BsrGI TGTACA 1 cut(s) 972
BsrI ACTGG 4 cut(s) 76, 684, 949, 1125
BssAI RCCGGY 2 cut(s) 43, 775
BssMI GATC 5 cut(s) 86, 179, 195, 790, 1051
Bst2UI CCWGG 2 cut(s) 854, 881
Bst4CI ACNGT 5 cut(s) 112, 136, 362, 620, 1062
Bst6I CTCTTC 1 cut(s) 762
BstAPI GCANNNNNTGC 2 cut(s) 689, 1096
BstAUI TGTACA 1 cut(s) 972
BstC8I GCNNGC 2 cut(s) 341, 773
BstDEI CTNAG 3 cut(s) 512, 741, 1227
BstEII GGTNACC 1 cut(s) 254
BstF5I GGATG 6 cut(s) 580, 636, 823, 1047, 1167, 1358
BstFNI CGCG 1 cut(s) 382
BstHHI GCGC 2 cut(s) 384, 694
BstKTI GATC 5 cut(s) 89, 182, 198, 793, 1054
BstMAI GTCTC 3 cut(s) 111, 662, 1111
BstMBI GATC 5 cut(s) 86, 179, 195, 790, 1051
BstMWI GCNNNNNNNGC 8 cut(s) 272, 345, 390, 660, 689, 915, 1096, 1269
BstNI CCWGG 2 cut(s) 854, 881
BstNSI RCATGY 1 cut(s) 206
BstPI GGTNACC 1 cut(s) 254
BstSCI CCNGG 3 cut(s) 521, 852, 879
BstSLI GKGCMC 2 cut(s) 627, 1316
BstUI CGCG 1 cut(s) 382
BstV1I GCAGC 6 cut(s) 335, 405, 457, 998, 1269, 1272
BstX2I RGATCY 3 cut(s) 179, 790, 1051
BstXI CCANNNNNNTGG 1 cut(s) 620
BstYI RGATCY 3 cut(s) 179, 790, 1051
Bsu15I ATCGAT 1 cut(s) 85
BsuRI GGCC 5 cut(s) 377, 534, 746, 852, 1046
BsuTUI ATCGAT 1 cut(s) 85
BtsCI GGATG 6 cut(s) 580, 636, 823, 1047, 1167, 1358
BtsI GCAGTG 1 cut(s) 1174
BtsIMutI CAGTG 3 cut(s) 367, 1067, 1174
Cac8I GCNNGC 2 cut(s) 341, 773
CaiI CAGNNNCTG 1 cut(s) 1120
CfoI GCGC 2 cut(s) 384, 694
Cfr10I RCCGGY 2 cut(s) 43, 775
Cfr13I GGNCC 4 cut(s) 745, 866, 883, 1070
ClaI ATCGAT 1 cut(s) 85
Csp6I GTAC 4 cut(s) 646, 973, 1275, 1288
CspAI ACCGGT 1 cut(s) 43
CspCI CAANNNNNGTGG 2 cut(s) 132, 167
CviAII CATG 7 cut(s) 203, 323, 536, 565, 614, 649, 767
CviQI GTAC 4 cut(s) 646, 973, 1275, 1288
DdeI CTNAG 3 cut(s) 512, 741, 1227
DpnI GATC 5 cut(s) 88, 181, 197, 792, 1053
DpnII GATC 5 cut(s) 86, 179, 195, 790, 1051
EaeI YGGCCR 2 cut(s) 375, 1044
Eam1104I CTCTTC 1 cut(s) 762
EarI CTCTTC 1 cut(s) 762
EciI GGCGGA 1 cut(s) 432
Eco47I GGWCC 3 cut(s) 866, 883, 1070
Eco57I CTGAAG 1 cut(s) 1236
Eco91I GGTNACC 1 cut(s) 254
EcoO109I RGGNCCY 1 cut(s) 745
EcoO65I GGTNACC 1 cut(s) 254
EcoRII CCWGG 2 cut(s) 852, 879
FaeI CATG 7 cut(s) 206, 326, 539, 568, 617, 652, 770
FaqI GGGAC 2 cut(s) 501, 538
FatI CATG 7 cut(s) 202, 322, 535, 564, 613, 648, 766
Fnu4HI GCNGC 7 cut(s) 349, 394, 471, 695, 1012, 1258, 1261
FokI GGATG 5 cut(s) 567, 643, 830, 1054, 1154
Fsp4HI GCNGC 7 cut(s) 349, 394, 471, 695, 1012, 1258, 1261
FspBI CTAG 5 cut(s) 234, 602, 749, 858, 1341
GlaI GCGC 2 cut(s) 383, 693
GluI GCNGC 7 cut(s) 349, 394, 471, 695, 1012, 1258, 1261
GsaI CCCAGC 1 cut(s) 279
HaeIII GGCC 5 cut(s) 377, 534, 746, 852, 1046
HapII CCGG 5 cut(s) 44, 522, 776, 929, 1047
HhaI GCGC 2 cut(s) 384, 694
Hin1II CATG 7 cut(s) 206, 326, 539, 568, 617, 652, 770
Hin6I GCGC 2 cut(s) 382, 692
HinP1I GCGC 2 cut(s) 382, 692
HindIII AAGCTT 2 cut(s) 661, 798
HinfI GANTC 6 cut(s) 464, 489, 670, 717, 752, 901
HpaII CCGG 5 cut(s) 44, 522, 776, 929, 1047
HphI GGTGA 2 cut(s) 33, 437
Hpy166II GTNNAC 3 cut(s) 49, 301, 973
Hpy188I TCNGA 7 cut(s) 515, 795, 844, 877, 1036, 1281, 1359
Hpy188III TCNNGA 2 cut(s) 29, 191
Hpy8I GTNNAC 3 cut(s) 49, 301, 973
HpyAV CCTTC 2 cut(s) 1119, 1228
HpyCH4III ACNGT 5 cut(s) 112, 136, 362, 620, 1062
HpyCH4IV ACGT 1 cut(s) 51
HpyCH4V TGCA 9 cut(s) 15, 339, 456, 473, 570, 833, 926, 1083, 1099
HpyF10VI GCNNNNNNNGC 8 cut(s) 272, 345, 390, 660, 689, 915, 1096, 1269
HpyF3I CTNAG 3 cut(s) 512, 741, 1227
HpySE526I ACGT 1 cut(s) 51
Hsp92II CATG 7 cut(s) 206, 326, 539, 568, 617, 652, 770
HspAI GCGC 2 cut(s) 382, 692
Kzo9I GATC 5 cut(s) 86, 179, 195, 790, 1051
LmnI GCTCC 1 cut(s) 708
Lsp1109I GCAGC 6 cut(s) 335, 405, 457, 998, 1269, 1272
LweI GCATC 1 cut(s) 1108
MaeI CTAG 5 cut(s) 234, 602, 749, 858, 1341
MaeII ACGT 1 cut(s) 51
MaeIII GTNAC 6 cut(s) 254, 356, 362, 449, 952, 1265
MalI GATC 5 cut(s) 88, 181, 197, 792, 1053
MboI GATC 5 cut(s) 86, 179, 195, 790, 1051
MboII GAAGA 4 cut(s) 17, 726, 749, 1036
MfeI CAATTG 1 cut(s) 729
MflI RGATCY 3 cut(s) 179, 790, 1051
MhlI GDGCHC 2 cut(s) 627, 1316
MlsI TGGCCA 1 cut(s) 377
MluCI AATT 6 cut(s) 7, 261, 386, 400, 560, 729
MluNI TGGCCA 1 cut(s) 377
MlyI GAGTC 3 cut(s) 458, 483, 664
MmeI TCCRAC 3 cut(s) 94, 795, 975
MnlI CCTC 7 cut(s) 151, 286, 879, 1138, 1181, 1326, 1329
Mox20I TGGCCA 1 cut(s) 377
MroXI GAANNNNTTC 1 cut(s) 127
MscI TGGCCA 1 cut(s) 377
MseI TTAA 2 cut(s) 99, 404
MslI CAYNNNNRTG 2 cut(s) 618, 765
Msp20I TGGCCA 1 cut(s) 377
MspI CCGG 5 cut(s) 44, 522, 776, 929, 1047
MspR9I CCNGG 3 cut(s) 523, 854, 881
MunI CAATTG 1 cut(s) 729
Mva1269I GAATGC 1 cut(s) 694
MvaI CCWGG 2 cut(s) 854, 881
MvnI CGCG 1 cut(s) 382
MwoI GCNNNNNNNGC 8 cut(s) 272, 345, 390, 660, 689, 915, 1096, 1269
NciI CCSGG 1 cut(s) 523
NdeII GATC 5 cut(s) 86, 179, 195, 790, 1051
NlaIII CATG 7 cut(s) 206, 326, 539, 568, 617, 652, 770
NlaIV GGNNCC 1 cut(s) 910
NmuCI GTSAC 1 cut(s) 362
NspI RCATGY 1 cut(s) 206
PciI ACATGT 1 cut(s) 202
PctI GAATGC 1 cut(s) 694
PdmI GAANNNNTTC 1 cut(s) 127
PfeI GAWTC 3 cut(s) 717, 752, 901
PflMI CCANNNNNTGG 1 cut(s) 1120
PinAI ACCGGT 1 cut(s) 43
PkrI GCNGC 7 cut(s) 350, 395, 472, 696, 1013, 1259, 1262
PleI GAGTC 3 cut(s) 458, 483, 664
PpsI GAGTC 3 cut(s) 458, 483, 664
PscI ACATGT 1 cut(s) 202
Psp6I CCWGG 2 cut(s) 852, 879
PspEI GGTNACC 1 cut(s) 254
PspFI CCCAGC 1 cut(s) 275
PspGI CCWGG 2 cut(s) 852, 879
PspN4I GGNNCC 1 cut(s) 910
PspPI GGNCC 4 cut(s) 745, 866, 883, 1070
PstNI CAGNNNCTG 1 cut(s) 1120
PsuI RGATCY 3 cut(s) 179, 790, 1051
RsaI GTAC 4 cut(s) 647, 974, 1276, 1289
RsaNI GTAC 4 cut(s) 646, 973, 1275, 1288
RseI CAYNNNNRTG 2 cut(s) 618, 765
SaqAI TTAA 2 cut(s) 99, 404
SatI GCNGC 7 cut(s) 349, 394, 471, 695, 1012, 1258, 1261
Sau3AI GATC 5 cut(s) 86, 179, 195, 790, 1051
Sau96I GGNCC 4 cut(s) 745, 866, 883, 1070
ScaI AGTACT 1 cut(s) 1276
SchI GAGTC 3 cut(s) 458, 483, 664
ScrFI CCNGG 3 cut(s) 523, 854, 881
SduI GDGCHC 2 cut(s) 627, 1316
SfaNI GCATC 1 cut(s) 1108
SinI GGWCC 3 cut(s) 866, 883, 1070
SmiMI CAYNNNNRTG 2 cut(s) 618, 765
Sse9I AATT 6 cut(s) 7, 261, 386, 400, 560, 729
SsiI CCGC 5 cut(s) 75, 417, 695, 886, 1160
SspI AATATT 2 cut(s) 289, 1031
SspMI CTAG 5 cut(s) 234, 602, 749, 858, 1341
StyD4I CCNGG 3 cut(s) 521, 852, 879
TaaI ACNGT 5 cut(s) 112, 136, 362, 620, 1062
TaiI ACGT 1 cut(s) 54
TaqI TCGA 2 cut(s) 85, 268
TasI AATT 6 cut(s) 7, 261, 386, 400, 560, 729
TatI WGTACW 3 cut(s) 645, 972, 1274
TauI GCSGC 1 cut(s) 697
TfiI GAWTC 3 cut(s) 717, 752, 901
Tru1I TTAA 2 cut(s) 99, 404
Tru9I TTAA 2 cut(s) 99, 404
TscAI CASTG 3 cut(s) 367, 1067, 1181
TseFI GTSAC 1 cut(s) 362
TseI GCWGC 6 cut(s) 348, 393, 470, 1011, 1257, 1260
Tsp45I GTSAC 1 cut(s) 362
TspDTI ATGAA 5 cut(s) 311, 552, 553, 567, 773
TspRI CASTG 3 cut(s) 367, 1067, 1181
Van91I CCANNNNNTGG 1 cut(s) 1120
VpaK11BI GGWCC 3 cut(s) 866, 883, 1070
XapI RAATTY 3 cut(s) 386, 400, 560
XceI RCATGY 1 cut(s) 206
XcmI CCANNNNNNNNNTGG 1 cut(s) 811
XmnI GAANNNNTTC 1 cut(s) 127
XspI CTAG 5 cut(s) 234, 602, 749, 858, 1341
ZrmI AGTACT 1 cut(s) 1276
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.