Prupe.1G270600_v2.0.a1

Protein kinase 2B, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0017292
Physical Location & Seq
Forward (+)
27787467 .. 27789996
2530 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G270600.1

Sequence Viewer

Length: 1377 bp
ATGGGAAATTGCTTGAGGAAAAAGATTCAAGAGCCAGAGTCACCAGTTGCCATTATTCCAAGGCCTCCCACTGGGGACATTGGGTTACAAGTTTTTCTTCCCGAGAAGAAGATTAATGGAACAGTTGAAGAGAGAGCTCCAACTGCTTCCCCAACTTTCTTACAAGGTCAAGAAAAGCGGAGTATACGTGAAAGACGTAGATTACCACTTCAAGAAAGGAATTCAATCGTCTGTTCAAACACAGATATAGGTGCAAAAAAGATTGGTAAAAATACTAGGCATGGTTATACAGCCACTCCAAGTCCAAAAAATTGGACTTTGGAGACGCTGACTGGGAAGGAAGGAGGGTTCCGGGATTACAAGCTTTACTGCTTTTGTTATAGTTCCTTGAAAGCTGCCACACGCAAATTTAGCAGTAAGAACTTAATCGGACAAGGCGGATTCGGAGATGTCTACAAAGGGTATGTCAGTTATTGCAACATGAACTCCGCTGCAAAACCAAGTGAAGGATTTCCTATTGCTATCAAAAGGCTCAGGAAGACTGGAGCACAAGGCCATGAACAATGGGAGAATGAGAGGAAATTTATGAGCAAAATAAGCCATCCGAACATAGTGAAGCTAATAGGGTACTGCTGCGAAGGTGAGCACAGAATGTTGGTCTATGAGTATATGAGCGGAGGAAGCTTGGAGGCCCAACTCATGACAGAAAATGCTACACAACTAGATTGGAGCAGAAGAATCAAAGTAGCACTTGGGGCTGCCAAGGCTCTGAATTGTCTTCACACTCATAAGACGCCTTTCATTCATCGCGATCTAAAAGCTTCGAATGTTCTGTTGGATGATGACTGGAATGCTAAGCTTTCAGACTTTGGCCTTGCTAAATATGGACCCCGAGATGGCCAGGACCATGTAATGACAAGGATTCTTGGCACCAAAGGCTACATTGCACCTGAATACATAGGGACAGGGCATGTGACACTCAAAACCGATGTATACAGCTTTGGTGTGGTGCTTTTGGAGATTTTGTCAGGCTCCTGTGCCGTGAAGAAATACTCGGATGGGATGGCGGGTGATCTAACCAAGTGGGCTGAACCATATCTTAGCAACAGGCAGAAGCTGCATCATGTGATTGACCAGAGACTTGGAAATAACTTCCCTGTGGAAGAAGCTCACAAGTTTGCTGAGCTCATCCTCCGGTGCCTTGATTCAGACCCCAAGAGCAGACCAACAATGGCTGAAGTTGTGGGTGATTTGGAGCAGTTACAAGAAAACAGAAGCAGCAGCAGCAGCAATCGGGTCTCAGTCCATGTTACTACGCTCACCCCATGCCCTCCTTACAGAAGCATTTTCCCTGGACGAAATGGATGTCAGGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

459

Amino Acids

51.1

Weight (kDa)

9.27

Isoelectric Point (pI)

42.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017292)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g31690
malus_domestica MD13G1076200.v1.1 MD16G1076400.v1.1
prunus_persica Prupe.1G270600_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0440201
rosa_laevigata RLG00000006196
rosa_multiflora Rmu_sc0001685.1_g000028
rosa_roxburghii Rroxscaffold_5G00381130
rosa_rugosa Rorug04G0325200 Rorug04G0325300.1
rosa_samantha Rh4AG376000 Rh4DG382800
rosa_wichuraiana Rw4G032180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 929, 1197
AccBSI CCGCTC 1 cut(s) 675
AccI GTMKAC 3 cut(s) 184, 453, 993
AccII CGCG 1 cut(s) 810
AciI CCGC 5 cut(s) 178, 438, 489, 675, 1067
AcoI YGGCCR 1 cut(s) 898
AcsI RAATTY 3 cut(s) 220, 407, 581
AcuI CTGAAG 1 cut(s) 1257
AcyI GRCGYC 1 cut(s) 794
AfaI GTAC 1 cut(s) 629
AfiI CCNNNNNNNGG 3 cut(s) 71, 506, 896
AgsI TTSAA 6 cut(s) 29, 128, 212, 225, 237, 391
AjnI CCWGG 2 cut(s) 900, 1351
AjuI GAANNNNNNNTTGG 2 cut(s) 818, 850
AloI GAACNNNNNNTCC 2 cut(s) 332, 364
Alw21I GWGCWC 4 cut(s) 139, 550, 648, 1188
Alw26I GTCTC 3 cut(s) 317, 1132, 1303
AlwNI CAGNNNCTG 1 cut(s) 1117
Ama87I CYCGRG 2 cut(s) 101, 891
AoxI GGCC 5 cut(s) 62, 553, 690, 871, 898
ApeKI GCWGC 9 cut(s) 395, 491, 633, 758, 1117, 1278, 1281, 1284, 1287
ApoI RAATTY 3 cut(s) 220, 407, 581
ArsI GACNNNNNNTTYG 3 cut(s) 450, 482, 1351
AseI ATTAAT 1 cut(s) 114
Asp700I GAANNNNTTC 1 cut(s) 510
AspS9I GGNCC 3 cut(s) 691, 887, 904
AsuC2I CCSGG 1 cut(s) 353
AsuHPI GGTGA 5 cut(s) 33, 653, 1082, 1259, 1312
AsuII TTCGAA 1 cut(s) 824
AvaI CYCGRG 2 cut(s) 101, 891
AvaII GGWCC 2 cut(s) 887, 904
BalI TGGCCA 1 cut(s) 900
BanI GGYRCC 2 cut(s) 929, 1197
BanII GRGCYC 2 cut(s) 139, 1188
BbsI GAAGAC 2 cut(s) 545, 770
Bbv12I GWGCWC 4 cut(s) 139, 550, 648, 1188
BbvI GCAGC 9 cut(s) 382, 478, 620, 745, 1104, 1290, 1293, 1296, 1299
BccI CCATC 4 cut(s) 609, 890, 1052, 1057
BceAI ACGGC 1 cut(s) 1025
BciT130I CCWGG 2 cut(s) 902, 1353
BcnI CCSGG 1 cut(s) 353
BcoDI GTCTC 3 cut(s) 317, 1132, 1303
BfaI CTAG 2 cut(s) 276, 722
BisI GCNGC 9 cut(s) 396, 492, 634, 759, 1118, 1279, 1282, 1285, 1288
BlpI GCTNAGC 2 cut(s) 855, 1182
BlsI GCNGC 9 cut(s) 397, 493, 635, 760, 1119, 1280, 1283, 1286, 1289
Bme1390I CCNGG 3 cut(s) 353, 902, 1353
Bme18I GGWCC 2 cut(s) 887, 904
BmeT110I CYCGRG 2 cut(s) 101, 891
BmgT120I GGNCC 3 cut(s) 691, 887, 904
BmiI GGNNCC 5 cut(s) 350, 889, 931, 1033, 1199
BmrFI CCNGG 3 cut(s) 353, 902, 1353
BmrI ACTGGG 2 cut(s) 81, 342
BmsI GCATC 1 cut(s) 1129
BmuI ACTGGG 2 cut(s) 81, 342
BpiI GAAGAC 2 cut(s) 545, 770
BpmI CTGGAG 1 cut(s) 564
Bpu10I CCTNAGC 1 cut(s) 533
Bpu1102I GCTNAGC 2 cut(s) 855, 1182
Bpu14I TTCGAA 1 cut(s) 824
BpuEI CTTGAG 1 cut(s) 34
BpuMI CCSGG 1 cut(s) 353
BsaAI YACGTR 1 cut(s) 188
BsaHI GRCGYC 1 cut(s) 794
BsaI GGTCTC 1 cut(s) 1303
BsaJI CCNNGG 3 cut(s) 59, 762, 1351
BsaWI WCCGGW 1 cut(s) 1194
BsaXI ACNNNNNCTCC 8 cut(s) 172, 202, 280, 310, 470, 500, 1010, 1040
Bsc4I CCNNNNNNNGG 3 cut(s) 71, 506, 896
Bse1I ACTGG 5 cut(s) 44, 76, 337, 547, 851
Bse3DI GCAATG 1 cut(s) 942
BseBI CCWGG 2 cut(s) 902, 1353
BseDI CCNNGG 3 cut(s) 59, 762, 1351
BseGI GGATG 6 cut(s) 601, 844, 1063, 1068, 1188, 1370
BseLI CCNNNNNNNGG 3 cut(s) 71, 506, 896
BseMI GCAATG 1 cut(s) 942
BseMII CTCAG 3 cut(s) 547, 1173, 1314
BseNI ACTGG 5 cut(s) 44, 76, 337, 547, 851
BseXI GCAGC 9 cut(s) 382, 478, 620, 745, 1104, 1290, 1293, 1296, 1299
Bsh1236I CGCG 1 cut(s) 810
BshFI GGCC 5 cut(s) 64, 555, 692, 873, 900
BshNI GGYRCC 2 cut(s) 929, 1197
BsiHKAI GWGCWC 4 cut(s) 139, 550, 648, 1188
BsiHKCI CYCGRG 2 cut(s) 101, 891
BsiSI CCGG 2 cut(s) 352, 1195
BslFI GGGAC 2 cut(s) 89, 976
BslI CCNNNNNNNGG 3 cut(s) 71, 506, 896
BsmAI GTCTC 3 cut(s) 317, 1132, 1303
BsmBI CGTCTC 1 cut(s) 317
BsmFI GGGAC 2 cut(s) 89, 976
BsmI GAATGC 1 cut(s) 856
BsnI GGCC 5 cut(s) 64, 555, 692, 873, 900
Bso31I GGTCTC 1 cut(s) 1303
BsoBI CYCGRG 2 cut(s) 101, 891
Bsp119I TTCGAA 1 cut(s) 824
Bsp1286I GDGCHC 4 cut(s) 139, 550, 648, 1188
Bsp143I GATC 2 cut(s) 811, 1072
Bsp1720I GCTNAGC 2 cut(s) 855, 1182
Bsp68I TCGCGA 1 cut(s) 810
BspACI CCGC 5 cut(s) 178, 438, 489, 675, 1067
BspANI GGCC 5 cut(s) 64, 555, 692, 873, 900
BspCNI CTCAG 3 cut(s) 546, 1174, 1313
BspFNI CGCG 1 cut(s) 810
BspHI TCATGA 1 cut(s) 699
BspLI GGNNCC 5 cut(s) 350, 889, 931, 1033, 1199
BspT104I TTCGAA 1 cut(s) 824
BspT107I GGYRCC 2 cut(s) 929, 1197
BspTNI GGTCTC 1 cut(s) 1303
BsrBI CCGCTC 1 cut(s) 675
BsrDI GCAATG 1 cut(s) 942
BsrI ACTGG 5 cut(s) 44, 76, 337, 547, 851
BssECI CCNNGG 3 cut(s) 59, 762, 1351
BssMI GATC 2 cut(s) 811, 1072
BssNAI GTATAC 2 cut(s) 185, 994
BssNI GRCGYC 1 cut(s) 794
BssT1I CCWWGG 2 cut(s) 59, 762
Bst1107I GTATAC 2 cut(s) 185, 994
Bst2UI CCWGG 2 cut(s) 902, 1353
Bst4CI ACNGT 1 cut(s) 124
Bst6I CTCTTC 1 cut(s) 123
BstACI GRCGYC 1 cut(s) 794
BstAPI GCANNNNNTGC 1 cut(s) 1117
BstBAI YACGTR 1 cut(s) 188
BstBI TTCGAA 1 cut(s) 824
BstDEI CTNAG 5 cut(s) 533, 855, 1100, 1182, 1300
BstF5I GGATG 6 cut(s) 601, 844, 1063, 1068, 1188, 1370
BstFNI CGCG 1 cut(s) 810
BstKTI GATC 2 cut(s) 814, 1075
BstMAI GTCTC 3 cut(s) 317, 1132, 1303
BstMBI GATC 2 cut(s) 811, 1072
BstNI CCWGG 2 cut(s) 902, 1353
BstNSI RCATGY 1 cut(s) 974
BstSCI CCNGG 3 cut(s) 351, 900, 1351
BstUI CGCG 1 cut(s) 810
BstV1I GCAGC 9 cut(s) 382, 478, 620, 745, 1104, 1290, 1293, 1296, 1299
BstV2I GAAGAC 2 cut(s) 545, 770
BstXI CCANNNNNNTGG 2 cut(s) 312, 1142
BstZ17I GTATAC 2 cut(s) 185, 994
BsuRI GGCC 5 cut(s) 64, 555, 692, 873, 900
BtgZI GCGATG 1 cut(s) 791
BtsCI GGATG 6 cut(s) 601, 844, 1063, 1068, 1188, 1370
BtsIMutI CAGTG 1 cut(s) 69
BtuMI TCGCGA 1 cut(s) 810
CaiI CAGNNNCTG 1 cut(s) 1117
CciI TCATGA 1 cut(s) 699
Cfr13I GGNCC 3 cut(s) 691, 887, 904
CseI GACGC 2 cut(s) 334, 802
Csp6I GTAC 1 cut(s) 628
CviAII CATG 9 cut(s) 281, 481, 557, 700, 908, 971, 1124, 1307, 1326
CviQI GTAC 1 cut(s) 628
DdeI CTNAG 5 cut(s) 533, 855, 1100, 1182, 1300
DpnI GATC 2 cut(s) 813, 1074
DpnII GATC 2 cut(s) 811, 1072
EaeI YGGCCR 1 cut(s) 898
Eam1104I CTCTTC 1 cut(s) 123
EarI CTCTTC 1 cut(s) 123
EciI GGCGGA 1 cut(s) 453
Ecl136II GAGCTC 2 cut(s) 137, 1186
Eco130I CCWWGG 2 cut(s) 59, 762
Eco147I AGGCCT 1 cut(s) 64
Eco24I GRGCYC 2 cut(s) 139, 1188
Eco31I GGTCTC 1 cut(s) 1303
Eco47I GGWCC 2 cut(s) 887, 904
Eco53kI GAGCTC 2 cut(s) 137, 1186
Eco57I CTGAAG 1 cut(s) 1257
Eco88I CYCGRG 2 cut(s) 101, 891
EcoICRI GAGCTC 2 cut(s) 137, 1186
EcoRI GAATTC 1 cut(s) 220
EcoRII CCWGG 2 cut(s) 900, 1351
EcoT14I CCWWGG 2 cut(s) 59, 762
EcoT38I GRGCYC 2 cut(s) 139, 1188
ErhI CCWWGG 2 cut(s) 59, 762
Esp3I CGTCTC 1 cut(s) 317
FaeI CATG 9 cut(s) 284, 484, 560, 703, 911, 974, 1127, 1310, 1329
FalI AAGNNNNNCTT 4 cut(s) 81, 113, 735, 767
FaqI GGGAC 2 cut(s) 89, 976
FatI CATG 9 cut(s) 280, 480, 556, 699, 907, 970, 1123, 1306, 1325
FauI CCCGC 1 cut(s) 1060
FblI GTMKAC 3 cut(s) 184, 453, 993
Fnu4HI GCNGC 9 cut(s) 396, 492, 634, 759, 1118, 1279, 1282, 1285, 1288
FokI GGATG 5 cut(s) 588, 851, 1070, 1075, 1175
FriOI GRGCYC 2 cut(s) 139, 1188
Fsp4HI GCNGC 9 cut(s) 396, 492, 634, 759, 1118, 1279, 1282, 1285, 1288
FspBI CTAG 2 cut(s) 276, 722
GluI GCNGC 9 cut(s) 396, 492, 634, 759, 1118, 1279, 1282, 1285, 1288
GsuI CTGGAG 1 cut(s) 564
HaeIII GGCC 5 cut(s) 64, 555, 692, 873, 900
HapII CCGG 2 cut(s) 352, 1195
HgaI GACGC 2 cut(s) 334, 802
Hin1I GRCGYC 1 cut(s) 794
Hin1II CATG 9 cut(s) 284, 484, 560, 703, 911, 974, 1127, 1310, 1329
HindIII AAGCTT 4 cut(s) 362, 682, 819, 857
HinfI GANTC 6 cut(s) 25, 38, 441, 738, 922, 1205
HpaII CCGG 2 cut(s) 352, 1195
HphI GGTGA 5 cut(s) 33, 653, 1082, 1259, 1312
Hpy166II GTNNAC 3 cut(s) 185, 454, 994
Hpy188I TCNGA 7 cut(s) 431, 446, 606, 771, 865, 1057, 1210
Hpy188III TCNNGA 7 cut(s) 29, 101, 170, 212, 535, 700, 809
Hpy8I GTNNAC 3 cut(s) 185, 454, 994
HpyAV CCTTC 4 cut(s) 331, 335, 500, 632
HpyCH4III ACNGT 1 cut(s) 124
HpyCH4IV ACGT 2 cut(s) 187, 196
HpyCH4V TGCA 5 cut(s) 254, 477, 494, 947, 1120
HpyF3I CTNAG 5 cut(s) 533, 855, 1100, 1182, 1300
HpySE526I ACGT 2 cut(s) 187, 196
Hsp92I GRCGYC 1 cut(s) 794
Hsp92II CATG 9 cut(s) 284, 484, 560, 703, 911, 974, 1127, 1310, 1329
Kzo9I GATC 2 cut(s) 811, 1072
LmnI GCTCC 5 cut(s) 142, 545, 729, 1037, 1255
Lsp1109I GCAGC 9 cut(s) 382, 478, 620, 745, 1104, 1290, 1293, 1296, 1299
LweI GCATC 1 cut(s) 1129
MaeI CTAG 2 cut(s) 276, 722
MaeII ACGT 2 cut(s) 187, 196
MaeIII GTNAC 5 cut(s) 39, 84, 973, 1260, 1309
MalI GATC 2 cut(s) 813, 1074
MbiI CCGCTC 1 cut(s) 675
MboI GATC 2 cut(s) 811, 1072
MboII GAAGA 9 cut(s) 89, 118, 121, 140, 550, 747, 770, 1057, 1175
MhlI GDGCHC 4 cut(s) 139, 550, 648, 1188
MlsI TGGCCA 1 cut(s) 900
MluCI AATT 6 cut(s) 7, 220, 310, 407, 581, 772
MluNI TGGCCA 1 cut(s) 900
MlyI GAGTC 1 cut(s) 47
MmeI TCCRAC 2 cut(s) 164, 816
MnlI CCTC 8 cut(s) 9, 75, 338, 570, 671, 682, 1202, 1341
Mox20I TGGCCA 1 cut(s) 900
MroXI GAANNNNTTC 1 cut(s) 510
MscI TGGCCA 1 cut(s) 900
MseI TTAA 2 cut(s) 114, 425
Msp20I TGGCCA 1 cut(s) 900
MspA1I CMGCKG 1 cut(s) 491
MspI CCGG 2 cut(s) 352, 1195
MspR9I CCNGG 3 cut(s) 353, 902, 1353
Mva1269I GAATGC 1 cut(s) 856
MvaI CCWGG 2 cut(s) 902, 1353
MvnI CGCG 1 cut(s) 810
NciI CCSGG 1 cut(s) 353
NdeII GATC 2 cut(s) 811, 1072
NlaIII CATG 9 cut(s) 284, 484, 560, 703, 911, 974, 1127, 1310, 1329
NlaIV GGNNCC 5 cut(s) 350, 889, 931, 1033, 1199
NmuCI GTSAC 2 cut(s) 39, 973
NruI TCGCGA 1 cut(s) 810
NspI RCATGY 1 cut(s) 974
NspV TTCGAA 1 cut(s) 824
PagI TCATGA 1 cut(s) 699
PceI AGGCCT 1 cut(s) 64
PcsI WCGNNNNNNNCGW 1 cut(s) 193
PctI GAATGC 1 cut(s) 856
PdmI GAANNNNTTC 1 cut(s) 510
PfeI GAWTC 5 cut(s) 25, 441, 738, 922, 1205
PfoI TCCNGGA 1 cut(s) 351
PkrI GCNGC 9 cut(s) 397, 493, 635, 760, 1119, 1280, 1283, 1286, 1289
PleI GAGTC 1 cut(s) 46
PpsI GAGTC 1 cut(s) 46
Ppu21I YACGTR 1 cut(s) 188
PshBI ATTAAT 1 cut(s) 114
Psp124BI GAGCTC 2 cut(s) 139, 1188
Psp6I CCWGG 2 cut(s) 900, 1351
PspGI CCWGG 2 cut(s) 900, 1351
PspN4I GGNNCC 5 cut(s) 350, 889, 931, 1033, 1199
PspPI GGNCC 3 cut(s) 691, 887, 904
PstNI CAGNNNCTG 1 cut(s) 1117
RruI TCGCGA 1 cut(s) 810
RsaI GTAC 1 cut(s) 629
RsaNI GTAC 1 cut(s) 628
SacI GAGCTC 2 cut(s) 139, 1188
SaqAI TTAA 2 cut(s) 114, 425
SatI GCNGC 9 cut(s) 396, 492, 634, 759, 1118, 1279, 1282, 1285, 1288
Sau3AI GATC 2 cut(s) 811, 1072
Sau96I GGNCC 3 cut(s) 691, 887, 904
SchI GAGTC 1 cut(s) 47
ScrFI CCNGG 3 cut(s) 353, 902, 1353
SduI GDGCHC 4 cut(s) 139, 550, 648, 1188
SfaNI GCATC 1 cut(s) 1129
SfuI TTCGAA 1 cut(s) 824
SinI GGWCC 2 cut(s) 887, 904
SmlI CTYRAG 1 cut(s) 13
SmoI CTYRAG 1 cut(s) 13
Sse9I AATT 6 cut(s) 7, 220, 310, 407, 581, 772
SseBI AGGCCT 1 cut(s) 64
SsiI CCGC 5 cut(s) 178, 438, 489, 675, 1067
SspMI CTAG 2 cut(s) 276, 722
SstI GAGCTC 2 cut(s) 139, 1188
StuI AGGCCT 1 cut(s) 64
StyD4I CCNGG 3 cut(s) 351, 900, 1351
StyI CCWWGG 2 cut(s) 59, 762
TaaI ACNGT 1 cut(s) 124
TaiI ACGT 2 cut(s) 190, 199
TaqI TCGA 1 cut(s) 824
TasI AATT 6 cut(s) 7, 220, 310, 407, 581, 772
TfiI GAWTC 5 cut(s) 25, 441, 738, 922, 1205
Tru1I TTAA 2 cut(s) 114, 425
Tru9I TTAA 2 cut(s) 114, 425
TscAI CASTG 1 cut(s) 76
TseFI GTSAC 2 cut(s) 39, 973
TseI GCWGC 9 cut(s) 395, 491, 633, 758, 1117, 1278, 1281, 1284, 1287
Tsp45I GTSAC 2 cut(s) 39, 973
TspDTI ATGAA 4 cut(s) 497, 573, 790, 794
TspRI CASTG 1 cut(s) 76
VpaK11BI GGWCC 2 cut(s) 887, 904
VspI ATTAAT 1 cut(s) 114
XapI RAATTY 3 cut(s) 220, 407, 581
XceI RCATGY 1 cut(s) 974
XmiI GTMKAC 3 cut(s) 184, 453, 993
XmnI GAANNNNTTC 1 cut(s) 510
XspI CTAG 2 cut(s) 276, 722
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.