Rroxscaffold_5G00381130

Protein kinase 2B, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0017292
Physical Location & Seq
Reverse (-)
61203054 .. 61204543
1490 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00381130.1

Sequence Viewer

Length: 1173 bp
ATGGTTAATTACATGTATTCCATGGGTGCAGAGAAGCCTAGCAGAGAGAAAAGGCGTGGTTACCGAATTGCTCGAAAAGCTGGGAGTTCTAATATTGAGGGAGTTAACAAGAATGATGGAGTGTTTCATGCTTACTACTACTGCAAGCTACGCTGCTTTTATTACACTGACTTAAAAGTGGCCACGCGCAAATTTAGCAGCAGAAATTTAATCGGGCAAGGTGGATTTGGAGATGTATATAAAGGGTATGTGAGTTACTGCACTACGGACTCTGCTGCAAAACCAAATGAGGGACTCGCAATTGCCATCAAAAGGCTCAGAAAGACCGGGACACAAGGCCATGAACAATGGGAGAATGAAATCAAATTCATGAGCAAACTAAACCATCCCAACATAGTGAAGCTACTAGGCTACTGCCATGACGGTGGGCACAGGATGTTGGTTTATGAGTACATGAGCAAAGGAAGCTTGGAGACTCAACTACTGGCAGAGAATGCGCCGCAGTTAGATTGGAGCAGAAGAATTAATGTAGCAATTGGGTTAGCTAAGGCCCTAGATTCTCTTCACAATCATGGCACGCCGGTCATTCATAGAGATCTGAAAGCTTCCAATGTCCTGTTGGATGATGACTTCAATGCTAAGCTATCAGATTTTGGCCTGGCTAGATATGGACCTCTCTGTGACCAGGACCACATAATGACAAGGATTCTTGGCACCAAAGGCTATATTGCACCGGAATACATAGGAACTGGACAGGTAACGCTGAAAACCGATGTGTACAGCTTTGGAGTGGTGCTGTTGGAGATTTTATCAGGCTGCTGTGCCGTGAAGAAATATTCAGATGGGATGGCCGGAGATCTTATACAGTGGGCTGGACCATATCTTTGCAACAAGCAACAACTGCATCAAGTGATTGACCAGAGACTGGGAAGGAACTTCCCAAAGAAAGAGGCTCAAAAGTTTGCGGAACTCATCCTCCACTGCCTTGCTTCAAACCCCAAGAGCAGACCAACAATGACTGAAGTTATAGCCGAGTTGAAGGACTTGCAAGAAAATACAAGCAGCAGCAGCAGCAGCAGTACTCCGAACTCGTACCATACTCCTACATTTACCCCATGCCCTCCTCCATACAAAATACTTTTACCTAGACGAAATGGGTGTCAGACAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

390

Amino Acids

43.72

Weight (kDa)

9.11

Isoelectric Point (pI)

43.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 67 - 344 1.9e-48 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 69 - 346 4.7e-52 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017292)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g31690
malus_domestica MD13G1076200.v1.1 MD16G1076400.v1.1
prunus_persica Prupe.1G270600_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0440201
rosa_laevigata RLG00000006196
rosa_multiflora Rmu_sc0001685.1_g000028
rosa_roxburghii Rroxscaffold_5G00381130
rosa_rugosa Rorug04G0325200 Rorug04G0325300.1
rosa_samantha Rh4AG376000 Rh4DG382800
rosa_wichuraiana Rw4G032180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 713
AccB7I CCANNNNNTGG 1 cut(s) 925
AccII CGCG 1 cut(s) 187
AciI CCGC 2 cut(s) 500, 965
AcoI YGGCCR 2 cut(s) 180, 849
AcsI RAATTY 3 cut(s) 191, 205, 365
AcuI CTGAAG 1 cut(s) 1041
AfaI GTAC 4 cut(s) 452, 779, 1081, 1094
AfiI CCNNNNNNNGG 3 cut(s) 290, 312, 925
AflIII ACRYGT 1 cut(s) 12
AgsI TTSAA 3 cut(s) 634, 993, 1039
AjnI CCWGG 2 cut(s) 657, 684
AloI GAACNNNNNNTCC 2 cut(s) 960, 992
AluBI AGCT 8 cut(s) 80, 148, 403, 468, 545, 605, 643, 783
AluI AGCT 8 cut(s) 80, 148, 403, 468, 545, 605, 643, 783
Alw26I GTCTC 2 cut(s) 467, 916
AlwNI CAGNNNCTG 1 cut(s) 925
AoxI GGCC 5 cut(s) 180, 337, 549, 655, 849
ApeKI GCWGC 9 cut(s) 153, 198, 275, 816, 1062, 1065, 1068, 1071, 1074
ApoI RAATTY 3 cut(s) 191, 205, 365
ArsI GACNNNNNNTTYG 3 cut(s) 867, 899, 1144
AseI ATTAAT 1 cut(s) 525
AspLEI GCGC 2 cut(s) 189, 499
AspS9I GGNCC 4 cut(s) 550, 671, 688, 875
AsuC2I CCSGG 1 cut(s) 328
AvaII GGWCC 3 cut(s) 671, 688, 875
BaeGI GKGCMC 1 cut(s) 432
BalI TGGCCA 1 cut(s) 182
BanI GGYRCC 1 cut(s) 713
BbvI GCAGC 9 cut(s) 140, 210, 262, 803, 1074, 1077, 1080, 1083, 1086
BccI CCATC 5 cut(s) 110, 314, 393, 836, 841
BceAI ACGGC 1 cut(s) 809
BciT130I CCWGG 2 cut(s) 659, 686
BcnI CCSGG 1 cut(s) 328
BcoDI GTCTC 2 cut(s) 467, 916
BfaI CTAG 5 cut(s) 39, 407, 554, 663, 1146
BglII AGATCT 2 cut(s) 595, 856
BlpI GCTNAGC 1 cut(s) 639
BmcAI AGTACT 1 cut(s) 1081
Bme1390I CCNGG 3 cut(s) 328, 659, 686
Bme18I GGWCC 3 cut(s) 671, 688, 875
BmgT120I GGNCC 4 cut(s) 550, 671, 688, 875
BmiI GGNNCC 1 cut(s) 715
BmrFI CCNGG 3 cut(s) 328, 659, 686
BmrI ACTGGG 1 cut(s) 935
BmsI GCATC 1 cut(s) 913
BmuI ACTGGG 1 cut(s) 935
Bpu10I CCTNAGC 1 cut(s) 546
Bpu1102I GCTNAGC 1 cut(s) 639
BpuMI CCSGG 1 cut(s) 328
BsaJI CCNNGG 1 cut(s) 21
BsaWI WCCGGW 1 cut(s) 733
BsaXI ACNNNNNCTCC 2 cut(s) 960, 990
Bsc4I CCNNNNNNNGG 3 cut(s) 290, 312, 925
Bse118I RCCGGY 1 cut(s) 580
Bse1I ACTGG 3 cut(s) 489, 754, 930
BseBI CCWGG 2 cut(s) 659, 686
BseDI CCNNGG 1 cut(s) 21
BseGI GGATG 5 cut(s) 385, 441, 628, 852, 972
BseLI CCNNNNNNNGG 3 cut(s) 290, 312, 925
BseMII CTCAG 1 cut(s) 331
BseNI ACTGG 3 cut(s) 489, 754, 930
BseRI GAGGAG 1 cut(s) 1113
BseSI GKGCMC 1 cut(s) 432
BseXI GCAGC 9 cut(s) 140, 210, 262, 803, 1074, 1077, 1080, 1083, 1086
BseYI CCCAGC 1 cut(s) 80
BsgI GTGCAG 2 cut(s) 48, 244
Bsh1236I CGCG 1 cut(s) 187
BshFI GGCC 5 cut(s) 182, 339, 551, 657, 851
BshNI GGYRCC 1 cut(s) 713
BsiSI CCGG 4 cut(s) 327, 581, 734, 852
BslFI GGGAC 2 cut(s) 306, 343
BslI CCNNNNNNNGG 3 cut(s) 290, 312, 925
BsmAI GTCTC 2 cut(s) 467, 916
BsmFI GGGAC 2 cut(s) 306, 343
BsmI GAATGC 1 cut(s) 499
BsnI GGCC 5 cut(s) 182, 339, 551, 657, 851
Bsp1286I GDGCHC 1 cut(s) 432
Bsp1407I TGTACA 1 cut(s) 777
Bsp143I GATC 2 cut(s) 595, 856
Bsp1720I GCTNAGC 1 cut(s) 639
Bsp19I CCATGG 1 cut(s) 21
BspACI CCGC 2 cut(s) 500, 965
BspANI GGCC 5 cut(s) 182, 339, 551, 657, 851
BspCNI CTCAG 1 cut(s) 330
BspFNI CGCG 1 cut(s) 187
BspHI TCATGA 1 cut(s) 369
BspLI GGNNCC 1 cut(s) 715
BspT107I GGYRCC 1 cut(s) 713
BsrFI RCCGGY 1 cut(s) 580
BsrGI TGTACA 1 cut(s) 777
BsrI ACTGG 3 cut(s) 489, 754, 930
BssAI RCCGGY 1 cut(s) 580
BssECI CCNNGG 1 cut(s) 21
BssMI GATC 2 cut(s) 595, 856
BssT1I CCWWGG 1 cut(s) 21
Bst2UI CCWGG 2 cut(s) 659, 686
Bst4CI ACNGT 2 cut(s) 425, 867
Bst6I CTCTTC 1 cut(s) 567
BstAPI GCANNNNNTGC 2 cut(s) 494, 901
BstAUI TGTACA 1 cut(s) 777
BstC8I GCNNGC 2 cut(s) 146, 578
BstDEI CTNAG 3 cut(s) 317, 546, 639
BstDSI CCRYGG 1 cut(s) 21
BstEII GGTNACC 1 cut(s) 59
BstF5I GGATG 5 cut(s) 385, 441, 628, 852, 972
BstFNI CGCG 1 cut(s) 187
BstHHI GCGC 2 cut(s) 189, 499
BstKTI GATC 2 cut(s) 598, 859
BstMAI GTCTC 2 cut(s) 467, 916
BstMBI GATC 2 cut(s) 595, 856
BstNI CCWGG 2 cut(s) 659, 686
BstNSI RCATGY 1 cut(s) 16
BstPI GGTNACC 1 cut(s) 59
BstSCI CCNGG 3 cut(s) 326, 657, 684
BstSLI GKGCMC 1 cut(s) 432
BstUI CGCG 1 cut(s) 187
BstV1I GCAGC 9 cut(s) 140, 210, 262, 803, 1074, 1077, 1080, 1083, 1086
BstX2I RGATCY 2 cut(s) 595, 856
BstXI CCANNNNNNTGG 1 cut(s) 425
BstYI RGATCY 2 cut(s) 595, 856
BsuRI GGCC 5 cut(s) 182, 339, 551, 657, 851
BtgI CCRYGG 1 cut(s) 21
BtsCI GGATG 5 cut(s) 385, 441, 628, 852, 972
BtsI GCAGTG 1 cut(s) 979
BtsIMutI CAGTG 3 cut(s) 165, 872, 979
Cac8I GCNNGC 2 cut(s) 146, 578
CaiI CAGNNNCTG 1 cut(s) 925
CciI TCATGA 1 cut(s) 369
CfoI GCGC 2 cut(s) 189, 499
Cfr10I RCCGGY 1 cut(s) 580
Cfr13I GGNCC 4 cut(s) 550, 671, 688, 875
Csp6I GTAC 4 cut(s) 451, 778, 1080, 1093
CviAII CATG 9 cut(s) 13, 22, 128, 341, 370, 419, 454, 572, 1116
CviQI GTAC 4 cut(s) 451, 778, 1080, 1093
DdeI CTNAG 3 cut(s) 317, 546, 639
DpnI GATC 2 cut(s) 597, 858
DpnII GATC 2 cut(s) 595, 856
EaeI YGGCCR 2 cut(s) 180, 849
Eam1104I CTCTTC 1 cut(s) 567
EarI CTCTTC 1 cut(s) 567
Eco130I CCWWGG 1 cut(s) 21
Eco47I GGWCC 3 cut(s) 671, 688, 875
Eco57I CTGAAG 1 cut(s) 1041
Eco91I GGTNACC 1 cut(s) 59
EcoO109I RGGNCCY 1 cut(s) 550
EcoO65I GGTNACC 1 cut(s) 59
EcoRII CCWGG 2 cut(s) 657, 684
EcoT14I CCWWGG 1 cut(s) 21
ErhI CCWWGG 1 cut(s) 21
FaeI CATG 9 cut(s) 16, 25, 131, 344, 373, 422, 457, 575, 1119
FaqI GGGAC 2 cut(s) 306, 343
FatI CATG 9 cut(s) 12, 21, 127, 340, 369, 418, 453, 571, 1115
FokI GGATG 5 cut(s) 372, 448, 635, 859, 959
FspBI CTAG 5 cut(s) 39, 407, 554, 663, 1146
GlaI GCGC 2 cut(s) 188, 498
GsaI CCCAGC 1 cut(s) 84
HaeIII GGCC 5 cut(s) 182, 339, 551, 657, 851
HapII CCGG 4 cut(s) 327, 581, 734, 852
HhaI GCGC 2 cut(s) 189, 499
Hin1II CATG 9 cut(s) 16, 25, 131, 344, 373, 422, 457, 575, 1119
Hin6I GCGC 2 cut(s) 187, 497
HinP1I GCGC 2 cut(s) 187, 497
HincII GTYRAC 1 cut(s) 106
HindII GTYRAC 1 cut(s) 106
HindIII AAGCTT 2 cut(s) 466, 603
HinfI GANTC 5 cut(s) 269, 294, 475, 557, 706
HpaI GTTAAC 1 cut(s) 106
HpaII CCGG 4 cut(s) 327, 581, 734, 852
Hpy166II GTNNAC 2 cut(s) 106, 778
Hpy188I TCNGA 6 cut(s) 320, 600, 649, 841, 1086, 1164
Hpy188III TCNNGA 1 cut(s) 370
Hpy8I GTNNAC 2 cut(s) 106, 778
HpyAV CCTTC 2 cut(s) 924, 1033
HpyCH4III ACNGT 2 cut(s) 425, 867
HpyCH4V TGCA 8 cut(s) 29, 144, 261, 278, 731, 888, 904, 1048
HpyF3I CTNAG 3 cut(s) 317, 546, 639
Hsp92II CATG 9 cut(s) 16, 25, 131, 344, 373, 422, 457, 575, 1119
HspAI GCGC 2 cut(s) 187, 497
KspAI GTTAAC 1 cut(s) 106
Kzo9I GATC 2 cut(s) 595, 856
LmnI GCTCC 1 cut(s) 513
Lsp1109I GCAGC 9 cut(s) 140, 210, 262, 803, 1074, 1077, 1080, 1083, 1086
LweI GCATC 1 cut(s) 913
MaeI CTAG 5 cut(s) 39, 407, 554, 663, 1146
MaeIII GTNAC 4 cut(s) 59, 254, 680, 757
MalI GATC 2 cut(s) 597, 858
MboI GATC 2 cut(s) 595, 856
MboII GAAGA 3 cut(s) 531, 554, 841
MfeI CAATTG 2 cut(s) 300, 534
MflI RGATCY 2 cut(s) 595, 856
MhlI GDGCHC 1 cut(s) 432
MlsI TGGCCA 1 cut(s) 182
MluCI AATT 8 cut(s) 7, 66, 191, 205, 300, 365, 522, 534
MluNI TGGCCA 1 cut(s) 182
MlyI GAGTC 3 cut(s) 263, 288, 469
MmeI TCCRAC 2 cut(s) 600, 780
MnlI CCTC 7 cut(s) 91, 283, 684, 943, 986, 1131, 1134
Mox20I TGGCCA 1 cut(s) 182
MscI TGGCCA 1 cut(s) 182
MseI TTAA 5 cut(s) 6, 105, 173, 209, 525
MslI CAYNNNNRTG 2 cut(s) 423, 570
Msp20I TGGCCA 1 cut(s) 182
MspI CCGG 4 cut(s) 327, 581, 734, 852
MspR9I CCNGG 3 cut(s) 328, 659, 686
MunI CAATTG 2 cut(s) 300, 534
Mva1269I GAATGC 1 cut(s) 499
MvaI CCWGG 2 cut(s) 659, 686
MvnI CGCG 1 cut(s) 187
NciI CCSGG 1 cut(s) 328
NcoI CCATGG 1 cut(s) 21
NdeII GATC 2 cut(s) 595, 856
NlaIII CATG 9 cut(s) 16, 25, 131, 344, 373, 422, 457, 575, 1119
NlaIV GGNNCC 1 cut(s) 715
NmeAIII GCCGAG 1 cut(s) 1057
NmuCI GTSAC 1 cut(s) 680
NspI RCATGY 1 cut(s) 16
PagI TCATGA 1 cut(s) 369
PciI ACATGT 1 cut(s) 12
PctI GAATGC 1 cut(s) 499
PfeI GAWTC 2 cut(s) 557, 706
PflMI CCANNNNNTGG 1 cut(s) 925
PleI GAGTC 3 cut(s) 263, 288, 469
PpsI GAGTC 3 cut(s) 263, 288, 469
PscI ACATGT 1 cut(s) 12
PshBI ATTAAT 1 cut(s) 525
Psp6I CCWGG 2 cut(s) 657, 684
PspEI GGTNACC 1 cut(s) 59
PspFI CCCAGC 1 cut(s) 80
PspGI CCWGG 2 cut(s) 657, 684
PspN4I GGNNCC 1 cut(s) 715
PspPI GGNCC 4 cut(s) 550, 671, 688, 875
PstNI CAGNNNCTG 1 cut(s) 925
PsuI RGATCY 2 cut(s) 595, 856
RsaI GTAC 4 cut(s) 452, 779, 1081, 1094
RsaNI GTAC 4 cut(s) 451, 778, 1080, 1093
RseI CAYNNNNRTG 2 cut(s) 423, 570
SaqAI TTAA 5 cut(s) 6, 105, 173, 209, 525
Sau3AI GATC 2 cut(s) 595, 856
Sau96I GGNCC 4 cut(s) 550, 671, 688, 875
ScaI AGTACT 1 cut(s) 1081
SchI GAGTC 3 cut(s) 263, 288, 469
ScrFI CCNGG 3 cut(s) 328, 659, 686
SduI GDGCHC 1 cut(s) 432
SfaNI GCATC 1 cut(s) 913
SinI GGWCC 3 cut(s) 671, 688, 875
SmiMI CAYNNNNRTG 2 cut(s) 423, 570
Sse9I AATT 8 cut(s) 7, 66, 191, 205, 300, 365, 522, 534
SsiI CCGC 2 cut(s) 500, 965
SspI AATATT 2 cut(s) 94, 836
SspMI CTAG 5 cut(s) 39, 407, 554, 663, 1146
StyD4I CCNGG 3 cut(s) 326, 657, 684
StyI CCWWGG 1 cut(s) 21
TaaI ACNGT 2 cut(s) 425, 867
TaqI TCGA 1 cut(s) 73
TasI AATT 8 cut(s) 7, 66, 191, 205, 300, 365, 522, 534
TatI WGTACW 3 cut(s) 450, 777, 1079
TauI GCSGC 1 cut(s) 502
TfiI GAWTC 2 cut(s) 557, 706
Tru1I TTAA 5 cut(s) 6, 105, 173, 209, 525
Tru9I TTAA 5 cut(s) 6, 105, 173, 209, 525
TscAI CASTG 3 cut(s) 172, 872, 986
TseFI GTSAC 1 cut(s) 680
TseI GCWGC 9 cut(s) 153, 198, 275, 816, 1062, 1065, 1068, 1071, 1074
Tsp45I GTSAC 1 cut(s) 680
TspDTI ATGAA 5 cut(s) 116, 357, 358, 372, 578
TspGWI ACGGA 1 cut(s) 281
TspRI CASTG 3 cut(s) 172, 872, 986
Van91I CCANNNNNTGG 1 cut(s) 925
VpaK11BI GGWCC 3 cut(s) 671, 688, 875
VspI ATTAAT 1 cut(s) 525
XapI RAATTY 3 cut(s) 191, 205, 365
XceI RCATGY 1 cut(s) 16
XcmI CCANNNNNNNNNTGG 1 cut(s) 616
XspI CTAG 5 cut(s) 39, 407, 554, 663, 1146
ZrmI AGTACT 1 cut(s) 1081
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.