Rw4G032180

Protein kinase 2B, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0017292
Physical Location & Seq
Reverse (-)
59317075 .. 59319084
2010 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G032180.1

Sequence Viewer

Length: 1383 bp
ATGGGAAATTGTTGCAGAAAACATCTTCAAGAGCCAAACTCACCGGTTCACGTTATTTCTCAAACTCCTACTGGCTGTTCTCATCGATCCAAGAAAGTTAATAGAACAACAGTTGGAGACAGAACAACTTCAACTGTTTCCTCAACTTCTGTGGGAGAAACGAAGCGTGAAAAACAGAGATCCCCACTTCAAGAGATCACACATGTTGCTTCAAACACAGATATAGTGGGTGCAGAGAAGCCTAGCAGAGAGAAAAGGCGTGGTTACCGAATTGCTCGAAAAGCTGGGAGTTCTAATATTGAGGGAGTGAACAAGAATGATGGAGTGTTTCATGCTTACTACAACTGCAAGCTACGCTGCTTTTGTTACAGTGACTTGAAAGTGGCCACGCGCAAATTTAGCAGCAGAAATTTAATCGGGCAAGGCGGATTTGGTGATGTATATAAAGGGTATGTGAGTTACTGCACTATGGACTCTGCTGCAAAACCAAATAAGGGACTCGCAATAGCCATCAAAAGGCTCAGAAAGACCGGGACACAAGGCCATGAACAATGGGAGAATGAAATCAAATTCATGAGCAAACTAAACCATCCCAACATAGTGAAGCTACTAGGCTACTGCCATGACGGTGGGCACAGGATGTTGGTTTATGAGTACATGAGCAAAGGAAGCTTGGAGACTCAACTACTGGCAGGTAAGAAGAATGCGCCGCAGTTAGATTGGAGCAGAAGAATCAATGTAGCAATTGGGTTAGCTAAGGCCCTAGATTCTCTTCACAATCATGGCACGCCGGTCATTCATAGAGATCTGAAAGCTTCCAATGTCCTGTTGGATGATGACTTCAATGCAAAGCTATCAGATTTTGGCCTGGCTAGATATGGACCTCTCTGTGACCAGGACCGCATAATGACAAGGATTCTTGGCACCAAAGGCTATATTGCACCGGAATACATAGGAACTGGACAGGTAACGCTGAAAACCGATGTGTACAGCTTTGGAGTGGTGCTGTTGGAGATTTTATCAGGCTGCTGTGCTGTGAAGAAATATTCAGATGGGATGGCCGGAGATCTTATACAGTGGGCTGGACCATATCTTTGCAACAAGCAACAACTGCATCAAGTGATTGACCAGAGACTGGGAAGGAACTTCCCAAAGAAAGAGGCTCAAAAGTTTGCGGAACTCATCCTCCACTGCCTTGCTTCAAACCCCAAGAGCAGACCAACAATGACTGAAGTTGTAGCTGAGTTGAAGGACTTACAAGAAAATACAAGCAGCAGCAGTAACAGCAGTACTCCGAACTCGTACCATACTCCTACATTTACCCCGTGCCCTCCTCCATACAAAATACTTTTACCTAGACGAAATGGATGTCAGACAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

460

Amino Acids

51.13

Weight (kDa)

9.44

Isoelectric Point (pI)

41.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 135 - 414 5.5e-48 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 137 - 416 1.5e-51 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0017292)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g31690
malus_domestica MD13G1076200.v1.1 MD16G1076400.v1.1
prunus_persica Prupe.1G270600_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0440201
rosa_laevigata RLG00000006196
rosa_multiflora Rmu_sc0001685.1_g000028
rosa_roxburghii Rroxscaffold_5G00381130
rosa_rugosa Rorug04G0325200 Rorug04G0325300.1
rosa_samantha Rh4AG376000 Rh4DG382800
rosa_wichuraiana Rw4G032180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 683
AccB1I GGYRCC 1 cut(s) 923
AccB7I CCANNNNNTGG 1 cut(s) 1135
AccII CGCG 1 cut(s) 391
AciI CCGC 4 cut(s) 426, 710, 901, 1175
AclWI GGATC 2 cut(s) 81, 174
AcoI YGGCCR 2 cut(s) 384, 1059
AcsI RAATTY 3 cut(s) 395, 409, 569
AcuI CTGAAG 1 cut(s) 1251
AfaI GTAC 4 cut(s) 656, 989, 1291, 1304
AfiI CCNNNNNNNGG 3 cut(s) 494, 516, 1135
AflIII ACRYGT 1 cut(s) 202
AgeI ACCGGT 1 cut(s) 43
AgsI TTSAA 8 cut(s) 29, 132, 191, 213, 379, 844, 1203, 1249
AjnI CCWGG 2 cut(s) 867, 894
AloI GAACNNNNNNTCC 2 cut(s) 1170, 1202
AluBI AGCT 9 cut(s) 284, 352, 607, 672, 755, 815, 853, 993, 1241
AluI AGCT 9 cut(s) 284, 352, 607, 672, 755, 815, 853, 993, 1241
Alw26I GTCTC 3 cut(s) 111, 671, 1126
AlwI GGATC 2 cut(s) 81, 174
AlwNI CAGNNNCTG 1 cut(s) 1135
AoxI GGCC 5 cut(s) 384, 541, 759, 865, 1059
ApeKI GCWGC 6 cut(s) 357, 402, 479, 1026, 1272, 1275
ApoI RAATTY 3 cut(s) 395, 409, 569
ArsI GACNNNNNNTTYG 3 cut(s) 1077, 1109, 1354
AsiGI ACCGGT 1 cut(s) 43
Asp700I GAANNNNTTC 1 cut(s) 127
AspLEI GCGC 2 cut(s) 393, 709
AspS9I GGNCC 4 cut(s) 760, 881, 898, 1085
AsuC2I CCSGG 1 cut(s) 532
AsuHPI GGTGA 2 cut(s) 33, 446
AvaII GGWCC 3 cut(s) 881, 898, 1085
BaeGI GKGCMC 2 cut(s) 636, 1331
BalI TGGCCA 1 cut(s) 386
BanI GGYRCC 1 cut(s) 923
BbvI GCAGC 6 cut(s) 344, 414, 466, 1013, 1284, 1287
BccI CCATC 5 cut(s) 314, 518, 597, 1046, 1051
BciT130I CCWGG 2 cut(s) 869, 896
BcnI CCSGG 1 cut(s) 532
BcoDI GTCTC 3 cut(s) 111, 671, 1126
BfaI CTAG 5 cut(s) 243, 611, 764, 873, 1356
BfuAI ACCTGC 1 cut(s) 683
BglII AGATCT 2 cut(s) 805, 1066
BisI GCNGC 7 cut(s) 358, 403, 480, 710, 1027, 1273, 1276
BlsI GCNGC 7 cut(s) 359, 404, 481, 711, 1028, 1274, 1277
BmcAI AGTACT 1 cut(s) 1291
Bme1390I CCNGG 3 cut(s) 532, 869, 896
Bme18I GGWCC 3 cut(s) 881, 898, 1085
BmgT120I GGNCC 4 cut(s) 760, 881, 898, 1085
BmiI GGNNCC 1 cut(s) 925
BmrFI CCNGG 3 cut(s) 532, 869, 896
BmrI ACTGGG 1 cut(s) 1145
BmsI GCATC 1 cut(s) 1123
BmuI ACTGGG 1 cut(s) 1145
BplI GAGNNNNNCTC 2 cut(s) 23, 55
Bpu10I CCTNAGC 1 cut(s) 756
BpuMI CCSGG 1 cut(s) 532
Bsa29I ATCGAT 1 cut(s) 85
BsaWI WCCGGW 2 cut(s) 43, 943
BsaXI ACNNNNNCTCC 2 cut(s) 1170, 1200
Bsc4I CCNNNNNNNGG 3 cut(s) 494, 516, 1135
Bse118I RCCGGY 2 cut(s) 43, 790
Bse1I ACTGG 4 cut(s) 76, 693, 964, 1140
BseBI CCWGG 2 cut(s) 869, 896
BseCI ATCGAT 1 cut(s) 85
BseGI GGATG 6 cut(s) 589, 645, 838, 1062, 1182, 1373
BseLI CCNNNNNNNGG 3 cut(s) 494, 516, 1135
BseMII CTCAG 2 cut(s) 535, 1233
BseNI ACTGG 4 cut(s) 76, 693, 964, 1140
BseRI GAGGAG 1 cut(s) 1323
BseSI GKGCMC 2 cut(s) 636, 1331
BseXI GCAGC 6 cut(s) 344, 414, 466, 1013, 1284, 1287
BseYI CCCAGC 1 cut(s) 284
BsgI GTGCAG 2 cut(s) 252, 448
Bsh1236I CGCG 1 cut(s) 391
BshFI GGCC 5 cut(s) 386, 543, 761, 867, 1061
BshNI GGYRCC 1 cut(s) 923
BshTI ACCGGT 1 cut(s) 43
BshVI ATCGAT 1 cut(s) 85
BsiSI CCGG 5 cut(s) 44, 531, 791, 944, 1062
BslFI GGGAC 2 cut(s) 510, 547
BslI CCNNNNNNNGG 3 cut(s) 494, 516, 1135
BsmAI GTCTC 3 cut(s) 111, 671, 1126
BsmFI GGGAC 2 cut(s) 510, 547
BsmI GAATGC 1 cut(s) 709
BsnI GGCC 5 cut(s) 386, 543, 761, 867, 1061
Bsp1286I GDGCHC 2 cut(s) 636, 1331
Bsp1407I TGTACA 1 cut(s) 987
Bsp143I GATC 5 cut(s) 86, 179, 195, 805, 1066
BspACI CCGC 4 cut(s) 426, 710, 901, 1175
BspANI GGCC 5 cut(s) 386, 543, 761, 867, 1061
BspCNI CTCAG 2 cut(s) 534, 1234
BspDI ATCGAT 1 cut(s) 85
BspFNI CGCG 1 cut(s) 391
BspHI TCATGA 1 cut(s) 573
BspLI GGNNCC 1 cut(s) 925
BspMI ACCTGC 1 cut(s) 683
BspPI GGATC 2 cut(s) 81, 174
BspT107I GGYRCC 1 cut(s) 923
BsrFI RCCGGY 2 cut(s) 43, 790
BsrGI TGTACA 1 cut(s) 987
BsrI ACTGG 4 cut(s) 76, 693, 964, 1140
BssAI RCCGGY 2 cut(s) 43, 790
BssMI GATC 5 cut(s) 86, 179, 195, 805, 1066
Bst2UI CCWGG 2 cut(s) 869, 896
Bst4CI ACNGT 5 cut(s) 112, 136, 371, 629, 1077
Bst6I CTCTTC 1 cut(s) 777
BstAPI GCANNNNNTGC 1 cut(s) 1111
BstAUI TGTACA 1 cut(s) 987
BstC8I GCNNGC 2 cut(s) 350, 788
BstDEI CTNAG 3 cut(s) 521, 756, 1242
BstEII GGTNACC 1 cut(s) 263
BstF5I GGATG 6 cut(s) 589, 645, 838, 1062, 1182, 1373
BstFNI CGCG 1 cut(s) 391
BstHHI GCGC 2 cut(s) 393, 709
BstKTI GATC 5 cut(s) 89, 182, 198, 808, 1069
BstMAI GTCTC 3 cut(s) 111, 671, 1126
BstMBI GATC 5 cut(s) 86, 179, 195, 805, 1066
BstMWI GCNNNNNNNGC 7 cut(s) 281, 354, 399, 669, 930, 1111, 1284
BstNI CCWGG 2 cut(s) 869, 896
BstNSI RCATGY 1 cut(s) 206
BstPI GGTNACC 1 cut(s) 263
BstSCI CCNGG 3 cut(s) 530, 867, 894
BstSLI GKGCMC 2 cut(s) 636, 1331
BstUI CGCG 1 cut(s) 391
BstV1I GCAGC 6 cut(s) 344, 414, 466, 1013, 1284, 1287
BstX2I RGATCY 3 cut(s) 179, 805, 1066
BstXI CCANNNNNNTGG 1 cut(s) 629
BstYI RGATCY 3 cut(s) 179, 805, 1066
Bsu15I ATCGAT 1 cut(s) 85
BsuRI GGCC 5 cut(s) 386, 543, 761, 867, 1061
BsuTUI ATCGAT 1 cut(s) 85
BtsCI GGATG 6 cut(s) 589, 645, 838, 1062, 1182, 1373
BtsI GCAGTG 1 cut(s) 1189
BtsIMutI CAGTG 3 cut(s) 376, 1082, 1189
BveI ACCTGC 1 cut(s) 683
Cac8I GCNNGC 2 cut(s) 350, 788
CaiI CAGNNNCTG 1 cut(s) 1135
CciI TCATGA 1 cut(s) 573
CfoI GCGC 2 cut(s) 393, 709
Cfr10I RCCGGY 2 cut(s) 43, 790
Cfr13I GGNCC 4 cut(s) 760, 881, 898, 1085
ClaI ATCGAT 1 cut(s) 85
Csp6I GTAC 4 cut(s) 655, 988, 1290, 1303
CspAI ACCGGT 1 cut(s) 43
CspCI CAANNNNNGTGG 2 cut(s) 132, 167
CviAII CATG 7 cut(s) 203, 332, 545, 574, 623, 658, 782
CviQI GTAC 4 cut(s) 655, 988, 1290, 1303
DdeI CTNAG 3 cut(s) 521, 756, 1242
DpnI GATC 5 cut(s) 88, 181, 197, 807, 1068
DpnII GATC 5 cut(s) 86, 179, 195, 805, 1066
EaeI YGGCCR 2 cut(s) 384, 1059
Eam1104I CTCTTC 1 cut(s) 777
EarI CTCTTC 1 cut(s) 777
EciI GGCGGA 1 cut(s) 441
Eco47I GGWCC 3 cut(s) 881, 898, 1085
Eco57I CTGAAG 1 cut(s) 1251
Eco91I GGTNACC 1 cut(s) 263
EcoO109I RGGNCCY 1 cut(s) 760
EcoO65I GGTNACC 1 cut(s) 263
EcoRII CCWGG 2 cut(s) 867, 894
FaeI CATG 7 cut(s) 206, 335, 548, 577, 626, 661, 785
FaqI GGGAC 2 cut(s) 510, 547
FatI CATG 7 cut(s) 202, 331, 544, 573, 622, 657, 781
Fnu4HI GCNGC 7 cut(s) 358, 403, 480, 710, 1027, 1273, 1276
FokI GGATG 5 cut(s) 576, 652, 845, 1069, 1169
Fsp4HI GCNGC 7 cut(s) 358, 403, 480, 710, 1027, 1273, 1276
FspBI CTAG 5 cut(s) 243, 611, 764, 873, 1356
GlaI GCGC 2 cut(s) 392, 708
GluI GCNGC 7 cut(s) 358, 403, 480, 710, 1027, 1273, 1276
GsaI CCCAGC 1 cut(s) 288
HaeIII GGCC 5 cut(s) 386, 543, 761, 867, 1061
HapII CCGG 5 cut(s) 44, 531, 791, 944, 1062
HhaI GCGC 2 cut(s) 393, 709
Hin1II CATG 7 cut(s) 206, 335, 548, 577, 626, 661, 785
Hin6I GCGC 2 cut(s) 391, 707
HinP1I GCGC 2 cut(s) 391, 707
HindIII AAGCTT 2 cut(s) 670, 813
HinfI GANTC 6 cut(s) 473, 498, 679, 732, 767, 916
HpaII CCGG 5 cut(s) 44, 531, 791, 944, 1062
HphI GGTGA 2 cut(s) 33, 446
Hpy166II GTNNAC 3 cut(s) 49, 310, 988
Hpy188I TCNGA 6 cut(s) 524, 810, 859, 1051, 1296, 1374
Hpy188III TCNNGA 3 cut(s) 29, 191, 574
Hpy8I GTNNAC 3 cut(s) 49, 310, 988
HpyAV CCTTC 2 cut(s) 1134, 1243
HpyCH4III ACNGT 5 cut(s) 112, 136, 371, 629, 1077
HpyCH4IV ACGT 1 cut(s) 51
HpyCH4V TGCA 9 cut(s) 15, 233, 348, 465, 482, 848, 941, 1098, 1114
HpyF10VI GCNNNNNNNGC 7 cut(s) 281, 354, 399, 669, 930, 1111, 1284
HpyF3I CTNAG 3 cut(s) 521, 756, 1242
HpySE526I ACGT 1 cut(s) 51
Hsp92II CATG 7 cut(s) 206, 335, 548, 577, 626, 661, 785
HspAI GCGC 2 cut(s) 391, 707
Kzo9I GATC 5 cut(s) 86, 179, 195, 805, 1066
LmnI GCTCC 1 cut(s) 723
Lsp1109I GCAGC 6 cut(s) 344, 414, 466, 1013, 1284, 1287
LweI GCATC 1 cut(s) 1123
MaeI CTAG 5 cut(s) 243, 611, 764, 873, 1356
MaeII ACGT 1 cut(s) 51
MaeIII GTNAC 7 cut(s) 263, 365, 371, 458, 890, 967, 1280
MalI GATC 5 cut(s) 88, 181, 197, 807, 1068
MboI GATC 5 cut(s) 86, 179, 195, 805, 1066
MboII GAAGA 5 cut(s) 17, 712, 741, 764, 1051
MfeI CAATTG 1 cut(s) 744
MflI RGATCY 3 cut(s) 179, 805, 1066
MhlI GDGCHC 2 cut(s) 636, 1331
MlsI TGGCCA 1 cut(s) 386
MluCI AATT 6 cut(s) 7, 270, 395, 409, 569, 744
MluNI TGGCCA 1 cut(s) 386
MlyI GAGTC 3 cut(s) 467, 492, 673
MmeI TCCRAC 3 cut(s) 94, 810, 990
MnlI CCTC 7 cut(s) 151, 295, 894, 1153, 1196, 1341, 1344
Mox20I TGGCCA 1 cut(s) 386
MroXI GAANNNNTTC 1 cut(s) 127
MscI TGGCCA 1 cut(s) 386
MseI TTAA 2 cut(s) 99, 413
MslI CAYNNNNRTG 2 cut(s) 627, 780
Msp20I TGGCCA 1 cut(s) 386
MspI CCGG 5 cut(s) 44, 531, 791, 944, 1062
MspR9I CCNGG 3 cut(s) 532, 869, 896
MunI CAATTG 1 cut(s) 744
Mva1269I GAATGC 1 cut(s) 709
MvaI CCWGG 2 cut(s) 869, 896
MvnI CGCG 1 cut(s) 391
MwoI GCNNNNNNNGC 7 cut(s) 281, 354, 399, 669, 930, 1111, 1284
NciI CCSGG 1 cut(s) 532
NdeII GATC 5 cut(s) 86, 179, 195, 805, 1066
NlaIII CATG 7 cut(s) 206, 335, 548, 577, 626, 661, 785
NlaIV GGNNCC 1 cut(s) 925
NmuCI GTSAC 2 cut(s) 371, 890
NspI RCATGY 1 cut(s) 206
PagI TCATGA 1 cut(s) 573
PciI ACATGT 1 cut(s) 202
PctI GAATGC 1 cut(s) 709
PdmI GAANNNNTTC 1 cut(s) 127
PfeI GAWTC 3 cut(s) 732, 767, 916
PflMI CCANNNNNTGG 1 cut(s) 1135
PinAI ACCGGT 1 cut(s) 43
PkrI GCNGC 7 cut(s) 359, 404, 481, 711, 1028, 1274, 1277
PleI GAGTC 3 cut(s) 467, 492, 673
PpsI GAGTC 3 cut(s) 467, 492, 673
PscI ACATGT 1 cut(s) 202
Psp6I CCWGG 2 cut(s) 867, 894
PspEI GGTNACC 1 cut(s) 263
PspFI CCCAGC 1 cut(s) 284
PspGI CCWGG 2 cut(s) 867, 894
PspN4I GGNNCC 1 cut(s) 925
PspPI GGNCC 4 cut(s) 760, 881, 898, 1085
PstNI CAGNNNCTG 1 cut(s) 1135
PsuI RGATCY 3 cut(s) 179, 805, 1066
RsaI GTAC 4 cut(s) 656, 989, 1291, 1304
RsaNI GTAC 4 cut(s) 655, 988, 1290, 1303
RseI CAYNNNNRTG 2 cut(s) 627, 780
SaqAI TTAA 2 cut(s) 99, 413
SatI GCNGC 7 cut(s) 358, 403, 480, 710, 1027, 1273, 1276
Sau3AI GATC 5 cut(s) 86, 179, 195, 805, 1066
Sau96I GGNCC 4 cut(s) 760, 881, 898, 1085
ScaI AGTACT 1 cut(s) 1291
SchI GAGTC 3 cut(s) 467, 492, 673
ScrFI CCNGG 3 cut(s) 532, 869, 896
SduI GDGCHC 2 cut(s) 636, 1331
SfaNI GCATC 1 cut(s) 1123
SinI GGWCC 3 cut(s) 881, 898, 1085
SmiMI CAYNNNNRTG 2 cut(s) 627, 780
Sse9I AATT 6 cut(s) 7, 270, 395, 409, 569, 744
SsiI CCGC 4 cut(s) 426, 710, 901, 1175
SspI AATATT 2 cut(s) 298, 1046
SspMI CTAG 5 cut(s) 243, 611, 764, 873, 1356
StyD4I CCNGG 3 cut(s) 530, 867, 894
TaaI ACNGT 5 cut(s) 112, 136, 371, 629, 1077
TaiI ACGT 1 cut(s) 54
TaqI TCGA 2 cut(s) 85, 277
TasI AATT 6 cut(s) 7, 270, 395, 409, 569, 744
TatI WGTACW 3 cut(s) 654, 987, 1289
TauI GCSGC 1 cut(s) 712
TfiI GAWTC 3 cut(s) 732, 767, 916
Tru1I TTAA 2 cut(s) 99, 413
Tru9I TTAA 2 cut(s) 99, 413
TscAI CASTG 3 cut(s) 376, 1082, 1196
TseFI GTSAC 2 cut(s) 371, 890
TseI GCWGC 6 cut(s) 357, 402, 479, 1026, 1272, 1275
Tsp45I GTSAC 2 cut(s) 371, 890
TspDTI ATGAA 5 cut(s) 320, 561, 562, 576, 788
TspRI CASTG 3 cut(s) 376, 1082, 1196
Van91I CCANNNNNTGG 1 cut(s) 1135
VpaK11BI GGWCC 3 cut(s) 881, 898, 1085
XapI RAATTY 3 cut(s) 395, 409, 569
XceI RCATGY 1 cut(s) 206
XcmI CCANNNNNNNNNTGG 1 cut(s) 826
XmnI GAANNNNTTC 1 cut(s) 127
XspI CTAG 5 cut(s) 243, 611, 764, 873, 1356
ZrmI AGTACT 1 cut(s) 1291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.