MD13G1076200.v1.1

Protein kinase 2B, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0017292
Physical Location & Seq
Reverse (-)
5404303 .. 5406752
2450 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1076200.v1.1.491

Sequence Viewer

Length: 1137 bp
ATGCGTTTAAGTGCAGAAAAAAACCGTAGCAAAAGTGCAAGGGCCACCACAAACTCCAATACTCCGAGTCCAAAAGATCTGAATTTGGAGAGAGTGAACAAGAAGGACGGACTGTTGCGAGATTACAAGCTATACTGCTTCTGTTACAGTGCCTTGAAAGCTTCCACACGCAAGTTTAGCAGCAAGAACTTGATCGGGCAAGGCGGATTCGGTGATGTCTACAAAGGGTACGTGAGGTATTGTAATATGAAGGCTGCTGCAAAACCAAGTGAGGGGTTTCCAATTGCTATCAAAAGGCTTAGAAAGACAGCACTACAAGGCGATGAACAATGGGAGAATGAGAGGAAATTTATGAGCAAAATAAGTCATCCAAACATAGTGAAACTAATAGGGTACTGCTGCGAGGGTGAGCACAGAATGTTGGTTTATGAGTACATGAACGGAGGAAGCTTGGAGGCCCAACTCTTGGCAGAAAATGCGACACGGTTAGATTGGAGGAGAAGAACCAAAATAGCACTCGGCTCGGCCAAGGCCCTGCATTGTCTTCACACTCGTAGGGCGCCAGTCATTCATCGTGATCTCAAAGCTTCAAATGTTCTGTTAGATGATGACTTCAATGCCAAGCTATCAGACTTTGGCCTGGCAAAATATGGACCCAAAGATGACCAGGACCACATAATGTCAAGGGTTCTTGGCACCAAAGGCTACATCGCACCTGAGTACATAGGGACAGGGCATGTGACACTCAAAACGGACGTATACAGCTTCGGCGTGGTGCTCTTAGAGATCTTCTCAGGCTCCTGCGCCGTGAAGAAATATTCGGATGGAATGGCTGGTGATCTTACCAAGTGGGCTGAACCATATCTTAGCAACCGCCAACAACTGCATTGTGTGATTGATCAGAGACTCGGAAACAACTTCCCGGTGGAAAAAGCTCACAAGTTTGCTGAACTGATTCTCCGATGCCTCGATTCAAACCCCACGAACAGACCAACGATGAATGAAGTCGTGGCTGATTTGGAGGAGTTGCATGAAAACACAAGCAGCAATCGGATTTCAGACCATGTTACCGCATTTACACCATGCCCTCCTCCTTACAGAAGGATTTTCCCTACACGAAATGGATGTCAGGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

379

Amino Acids

42.62

Weight (kDa)

9.3

Isoelectric Point (pI)

34.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 60 - 338 2.1e-47 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 62 - 340 2.4e-51 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017292)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g31690
malus_domestica MD13G1076200.v1.1 MD16G1076400.v1.1
prunus_persica Prupe.1G270600_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0440201
rosa_laevigata RLG00000006196
rosa_multiflora Rmu_sc0001685.1_g000028
rosa_roxburghii Rroxscaffold_5G00381130
rosa_rugosa Rorug04G0325200 Rorug04G0325300.1
rosa_samantha Rh4AG376000 Rh4DG382800
rosa_wichuraiana Rw4G032180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 559, 695
AccB7I CCANNNNNTGG 1 cut(s) 466
AccI GTMKAC 2 cut(s) 219, 759
AciI CCGC 3 cut(s) 204, 874, 1071
AcoI YGGCCR 1 cut(s) 525
AcsI RAATTY 2 cut(s) 82, 347
AcyI GRCGYC 1 cut(s) 560
AfaI GTAC 4 cut(s) 230, 395, 434, 722
AfiI CCNNNNNNNGG 2 cut(s) 272, 466
AgsI TTSAA 4 cut(s) 157, 591, 616, 975
AjnI CCWGG 2 cut(s) 639, 666
AloI GAACNNNNNNTCC 2 cut(s) 942, 974
AluBI AGCT 7 cut(s) 130, 161, 450, 587, 625, 765, 935
AluI AGCT 7 cut(s) 130, 161, 450, 587, 625, 765, 935
Alw21I GWGCWC 2 cut(s) 414, 780
Alw26I GTCTC 1 cut(s) 898
AoxI GGCC 5 cut(s) 42, 456, 525, 531, 637
ApeKI GCWGC 5 cut(s) 180, 254, 257, 399, 1044
ApoI RAATTY 2 cut(s) 82, 347
ArsI GACNNNNNNTTYG 1 cut(s) 1111
Asp700I GAANNNNTTC 1 cut(s) 954
AspLEI GCGC 2 cut(s) 562, 806
AspS9I GGNCC 5 cut(s) 42, 457, 532, 653, 670
AsuC2I CCSGG 1 cut(s) 923
AsuHPI GGTGA 3 cut(s) 224, 419, 848
AvaII GGWCC 2 cut(s) 653, 670
BanI GGYRCC 2 cut(s) 559, 695
BbsI GAAGAC 1 cut(s) 536
Bbv12I GWGCWC 2 cut(s) 414, 780
BbvI GCAGC 5 cut(s) 192, 241, 244, 386, 1056
BccI CCATC 1 cut(s) 818
BceAI ACGGC 1 cut(s) 791
BcgI CGANNNNNNTGC 2 cut(s) 504, 538
BciT130I CCWGG 2 cut(s) 641, 668
BclI TGATCA 1 cut(s) 898
BcnI CCSGG 1 cut(s) 923
BcoDI GTCTC 1 cut(s) 898
BfoI RGCGCY 1 cut(s) 563
BglII AGATCT 2 cut(s) 76, 786
BisI GCNGC 5 cut(s) 181, 255, 258, 400, 1045
BlsI GCNGC 5 cut(s) 182, 256, 259, 401, 1046
Bme1390I CCNGG 3 cut(s) 641, 668, 923
Bme18I GGWCC 2 cut(s) 653, 670
BmgT120I GGNCC 5 cut(s) 42, 457, 532, 653, 670
BmiI GGNNCC 4 cut(s) 561, 655, 697, 799
BmrFI CCNGG 3 cut(s) 641, 668, 923
BmsI GCATC 1 cut(s) 953
BpiI GAAGAC 1 cut(s) 536
BplI GAGNNNNNCTC 2 cut(s) 776, 808
BpuMI CCSGG 1 cut(s) 923
BsaAI YACGTR 1 cut(s) 232
BsaHI GRCGYC 1 cut(s) 560
BsaJI CCNNGG 1 cut(s) 528
BsaXI ACNNNNNCTCC 2 cut(s) 942, 972
Bsc4I CCNNNNNNNGG 2 cut(s) 272, 466
Bse1I ACTGG 1 cut(s) 563
BseBI CCWGG 2 cut(s) 641, 668
BseDI CCNNGG 1 cut(s) 528
BseGI GGATG 3 cut(s) 367, 829, 1130
BseLI CCNNNNNNNGG 2 cut(s) 272, 466
BseMII CTCAG 2 cut(s) 708, 807
BseNI ACTGG 1 cut(s) 563
BseRI GAGGAG 3 cut(s) 511, 1037, 1080
BseXI GCAGC 5 cut(s) 192, 241, 244, 386, 1056
BsgI GTGCAG 1 cut(s) 33
BshFI GGCC 5 cut(s) 44, 458, 527, 533, 639
BshNI GGYRCC 2 cut(s) 559, 695
BsiHKAI GWGCWC 2 cut(s) 414, 780
BsiSI CCGG 1 cut(s) 923
BslFI GGGAC 1 cut(s) 742
BslI CCNNNNNNNGG 2 cut(s) 272, 466
BsmAI GTCTC 1 cut(s) 898
BsmFI GGGAC 1 cut(s) 742
BsnI GGCC 5 cut(s) 44, 458, 527, 533, 639
Bsp1286I GDGCHC 2 cut(s) 414, 780
Bsp143I GATC 6 cut(s) 76, 192, 577, 786, 838, 898
BspACI CCGC 3 cut(s) 204, 874, 1071
BspANI GGCC 5 cut(s) 44, 458, 527, 533, 639
BspCNI CTCAG 2 cut(s) 709, 806
BspLI GGNNCC 4 cut(s) 561, 655, 697, 799
BspT107I GGYRCC 2 cut(s) 559, 695
BsrI ACTGG 1 cut(s) 563
BssECI CCNNGG 1 cut(s) 528
BssMI GATC 6 cut(s) 76, 192, 577, 786, 838, 898
BssNAI GTATAC 1 cut(s) 760
BssNI GRCGYC 1 cut(s) 560
BssT1I CCWWGG 1 cut(s) 528
Bst1107I GTATAC 1 cut(s) 760
Bst2UI CCWGG 2 cut(s) 641, 668
Bst4CI ACNGT 4 cut(s) 26, 114, 149, 486
BstACI GRCGYC 1 cut(s) 560
BstAPI GCANNNNNTGC 1 cut(s) 476
BstBAI YACGTR 1 cut(s) 232
BstDEI CTNAG 5 cut(s) 299, 717, 781, 793, 866
BstF5I GGATG 3 cut(s) 367, 829, 1130
BstH2I RGCGCY 1 cut(s) 563
BstHHI GCGC 2 cut(s) 562, 806
BstKTI GATC 6 cut(s) 79, 195, 580, 789, 841, 901
BstMAI GTCTC 1 cut(s) 898
BstMBI GATC 6 cut(s) 76, 192, 577, 786, 838, 898
BstMWI GCNNNNNNNGC 4 cut(s) 158, 177, 476, 702
BstNI CCWGG 2 cut(s) 641, 668
BstNSI RCATGY 1 cut(s) 740
BstSCI CCNGG 3 cut(s) 639, 666, 921
BstV1I GCAGC 5 cut(s) 192, 241, 244, 386, 1056
BstV2I GAAGAC 1 cut(s) 536
BstX2I RGATCY 2 cut(s) 76, 786
BstYI RGATCY 2 cut(s) 76, 786
BstZ17I GTATAC 1 cut(s) 760
BsuRI GGCC 5 cut(s) 44, 458, 527, 533, 639
BtgZI GCGATG 2 cut(s) 336, 694
BtsCI GGATG 3 cut(s) 367, 829, 1130
BtsIMutI CAGTG 1 cut(s) 154
CfoI GCGC 2 cut(s) 562, 806
Cfr13I GGNCC 5 cut(s) 42, 457, 532, 653, 670
Csp6I GTAC 4 cut(s) 229, 394, 433, 721
CviAII CATG 5 cut(s) 436, 737, 1031, 1064, 1083
CviQI GTAC 4 cut(s) 229, 394, 433, 721
DdeI CTNAG 5 cut(s) 299, 717, 781, 793, 866
DinI GGCGCC 1 cut(s) 561
DpnI GATC 6 cut(s) 78, 194, 579, 788, 840, 900
DpnII GATC 6 cut(s) 76, 192, 577, 786, 838, 898
EaeI YGGCCR 1 cut(s) 525
EciI GGCGGA 1 cut(s) 219
Eco130I CCWWGG 1 cut(s) 528
Eco47I GGWCC 2 cut(s) 653, 670
EcoO109I RGGNCCY 1 cut(s) 532
EcoRII CCWGG 2 cut(s) 639, 666
EcoT14I CCWWGG 1 cut(s) 528
EgeI GGCGCC 1 cut(s) 561
EheI GGCGCC 1 cut(s) 561
ErhI CCWWGG 1 cut(s) 528
FaeI CATG 5 cut(s) 439, 740, 1034, 1067, 1086
FaqI GGGAC 1 cut(s) 742
FatI CATG 5 cut(s) 435, 736, 1030, 1063, 1082
FbaI TGATCA 1 cut(s) 898
FblI GTMKAC 2 cut(s) 219, 759
Fnu4HI GCNGC 5 cut(s) 181, 255, 258, 400, 1045
FokI GGATG 2 cut(s) 354, 836
Fsp4HI GCNGC 5 cut(s) 181, 255, 258, 400, 1045
GlaI GCGC 2 cut(s) 561, 805
GluI GCNGC 5 cut(s) 181, 255, 258, 400, 1045
HaeII RGCGCY 1 cut(s) 563
HaeIII GGCC 5 cut(s) 44, 458, 527, 533, 639
HapII CCGG 1 cut(s) 923
HhaI GCGC 2 cut(s) 562, 806
Hin1I GRCGYC 1 cut(s) 560
Hin1II CATG 5 cut(s) 439, 740, 1034, 1067, 1086
Hin6I GCGC 2 cut(s) 560, 804
HinP1I GCGC 2 cut(s) 560, 804
HindIII AAGCTT 3 cut(s) 159, 448, 585
HinfI GANTC 5 cut(s) 67, 207, 906, 955, 971
HpaII CCGG 1 cut(s) 923
HphI GGTGA 3 cut(s) 224, 419, 848
Hpy166II GTNNAC 3 cut(s) 97, 220, 760
Hpy188I TCNGA 9 cut(s) 66, 81, 631, 823, 903, 911, 962, 1053, 1060
Hpy188III TCNNGA 1 cut(s) 575
Hpy8I GTNNAC 3 cut(s) 97, 220, 760
HpyAV CCTTC 3 cut(s) 97, 244, 1095
HpyCH4III ACNGT 4 cut(s) 26, 114, 149, 486
HpyCH4IV ACGT 2 cut(s) 231, 756
HpyCH4V TGCA 6 cut(s) 14, 38, 260, 538, 886, 1030
HpyF10VI GCNNNNNNNGC 4 cut(s) 158, 177, 476, 702
HpyF3I CTNAG 5 cut(s) 299, 717, 781, 793, 866
HpySE526I ACGT 2 cut(s) 231, 756
Hsp92I GRCGYC 1 cut(s) 560
Hsp92II CATG 5 cut(s) 439, 740, 1034, 1067, 1086
HspAI GCGC 2 cut(s) 560, 804
KasI GGCGCC 1 cut(s) 559
Ksp22I TGATCA 1 cut(s) 898
Kzo9I GATC 6 cut(s) 76, 192, 577, 786, 838, 898
LmnI GCTCC 1 cut(s) 803
Lsp1109I GCAGC 5 cut(s) 192, 241, 244, 386, 1056
LweI GCATC 1 cut(s) 953
MaeII ACGT 2 cut(s) 231, 756
MaeIII GTNAC 3 cut(s) 143, 739, 1066
MalI GATC 6 cut(s) 78, 194, 579, 788, 840, 900
MboI GATC 6 cut(s) 76, 192, 577, 786, 838, 898
MboII GAAGA 4 cut(s) 513, 536, 781, 823
MfeI CAATTG 1 cut(s) 282
MflI RGATCY 2 cut(s) 76, 786
MhlI GDGCHC 2 cut(s) 414, 780
MluCI AATT 3 cut(s) 82, 282, 347
Mly113I GGCGCC 1 cut(s) 560
MlyI GAGTC 2 cut(s) 76, 900
MroXI GAANNNNTTC 1 cut(s) 954
MseI TTAA 1 cut(s) 8
MspI CCGG 1 cut(s) 923
MspR9I CCNGG 3 cut(s) 641, 668, 923
MunI CAATTG 1 cut(s) 282
MvaI CCWGG 2 cut(s) 641, 668
MwoI GCNNNNNNNGC 4 cut(s) 158, 177, 476, 702
NarI GGCGCC 1 cut(s) 560
NciI CCSGG 1 cut(s) 923
NdeII GATC 6 cut(s) 76, 192, 577, 786, 838, 898
NlaIII CATG 5 cut(s) 439, 740, 1034, 1067, 1086
NlaIV GGNNCC 4 cut(s) 561, 655, 697, 799
NmeAIII GCCGAG 2 cut(s) 498, 503
NmuCI GTSAC 1 cut(s) 739
NspI RCATGY 1 cut(s) 740
PdmI GAANNNNTTC 1 cut(s) 954
PfeI GAWTC 3 cut(s) 207, 955, 971
PflMI CCANNNNNTGG 1 cut(s) 466
PkrI GCNGC 5 cut(s) 182, 256, 259, 401, 1046
PleI GAGTC 2 cut(s) 75, 900
PluTI GGCGCC 1 cut(s) 563
PpsI GAGTC 2 cut(s) 75, 900
Ppu21I YACGTR 1 cut(s) 232
Psp6I CCWGG 2 cut(s) 639, 666
PspGI CCWGG 2 cut(s) 639, 666
PspN4I GGNNCC 4 cut(s) 561, 655, 697, 799
PspPI GGNCC 5 cut(s) 42, 457, 532, 653, 670
PsuI RGATCY 2 cut(s) 76, 786
RsaI GTAC 4 cut(s) 230, 395, 434, 722
RsaNI GTAC 4 cut(s) 229, 394, 433, 721
SaqAI TTAA 1 cut(s) 8
SatI GCNGC 5 cut(s) 181, 255, 258, 400, 1045
Sau3AI GATC 6 cut(s) 76, 192, 577, 786, 838, 898
Sau96I GGNCC 5 cut(s) 42, 457, 532, 653, 670
SchI GAGTC 2 cut(s) 76, 900
ScrFI CCNGG 3 cut(s) 641, 668, 923
SduI GDGCHC 2 cut(s) 414, 780
SfaNI GCATC 1 cut(s) 953
SfoI GGCGCC 1 cut(s) 561
SinI GGWCC 2 cut(s) 653, 670
Sse9I AATT 3 cut(s) 82, 282, 347
SsiI CCGC 3 cut(s) 204, 874, 1071
SspDI GGCGCC 1 cut(s) 559
SspI AATATT 1 cut(s) 818
StyD4I CCNGG 3 cut(s) 639, 666, 921
StyI CCWWGG 1 cut(s) 528
TaaI ACNGT 4 cut(s) 26, 114, 149, 486
TaiI ACGT 2 cut(s) 234, 759
TaqI TCGA 1 cut(s) 969
TasI AATT 3 cut(s) 82, 282, 347
TatI WGTACW 2 cut(s) 432, 720
TfiI GAWTC 3 cut(s) 207, 955, 971
Tru1I TTAA 1 cut(s) 8
Tru9I TTAA 1 cut(s) 8
TscAI CASTG 1 cut(s) 154
TseFI GTSAC 1 cut(s) 739
TseI GCWGC 5 cut(s) 180, 254, 257, 399, 1044
Tsp45I GTSAC 1 cut(s) 739
TspDTI ATGAA 7 cut(s) 263, 339, 452, 560, 1013, 1017, 1047
TspGWI ACGGA 3 cut(s) 123, 456, 767
TspRI CASTG 1 cut(s) 154
Van91I CCANNNNNTGG 1 cut(s) 466
VpaK11BI GGWCC 2 cut(s) 653, 670
XapI RAATTY 2 cut(s) 82, 347
XceI RCATGY 1 cut(s) 740
XmiI GTMKAC 2 cut(s) 219, 759
XmnI GAANNNNTTC 1 cut(s) 954
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.