MD16G1076400.v1.1

Protein kinase 2B, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0017292
Physical Location & Seq
Reverse (-)
5372739 .. 5376047
3309 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1076400.v1.1.491

Sequence Viewer

Length: 1191 bp
ATGATCAGTAAACGTGAGAAACGAAGATCACCACTTCAAGAGAGGAATTTCTCGGTCTCAGACATAGGTATGGTTATTGCATGCATGCAATCCAAAAAGCATGTAGCTGGTGCAGAAAAAAAACGTAGCAAAAATGCAAGGGCCACGACAAACTCCAATACTCCGAGTCCAAAAGATTGGAATTTGGAGAGAGTGAACAAGAAGGACGGAGTGTTGCGAGATTACAGGCTATACTGCTTCTGTTACAGTGCCTTGAAAGCTTCTACACGCAAGTTTAGCAGCAAGAACTTGATCGGGCAAGGCGGATTCGGTGATGTCTACAAAGGGTATGTAAGTTATTGTAATGTGAATGCTGCCGCAAAACCAAGTGAGGGATTTCCAATTGCTGTCAAAAGGCTCAGAAAGACAGCACTACAAGGCGATGAACAATGGGAGAATGAGAGGAAATTTATGAGCAAACTAAGTCATCCAAACATAGTGAAACTAATAGGGTACTGCCGCGAAGGTGAGCACAGAATGTTGGTTTATGAGTACATGAAAGGAGGAAGCTTGGAGGCCCAACTGATGGCGAAAAATGCTACACAGTTACACTGGAGGAGAAGAACCAAATTAGCACTCGGCGTAGCCAAGGCTCTGCATTGTCTTCACACTCGCGGGGCGCCCGTAATTCATCGTGATCTCAAAGCTTCAAATGTTCTGTTGGATGATGACTTCAATGCCAAGCTATCAGACTTTGGCCTGGCAAAATATGGACCCAAAGATGACCAGGACCACATAATGACAAGGGTTCTTGGTACCAAAGGCTACATCGCACCTGAGTACATAGCGACAGGGCATGTAACACTCAAAACCGACGTATACAGCTTCGGCGTGGTGCTCTTAGAGATCTTTTCAGGCTCCTGCGCCGTGAAGAAATATTCGGATGGGATGACTGGTGATCTTACCAAGTGGGCTGAACCGTATCTTCGCAACCGGCTACAACTGCATCGTGTGATTGATCAGAGACTTGGAAACAACTTCCCGGTGGAAGAAGCTCACAAGTTTGCTGAACTCATCCTCCGATGCCTCGATTCAAACCCTAAGAGCAGACCAACGATGACTGAGGTCGTAGCTGATTTGGAGGAGTTGCATGAAAACACAGGCAGCAATCGGATTTCAGGCCATGTTACGAAATGGGTGCCAGGCATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

397

Amino Acids

44.7

Weight (kDa)

9.6

Isoelectric Point (pI)

39.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 93 - 371 4.4e-49 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 95 - 373 5.6e-52 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017292)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g31690
malus_domestica MD13G1076200.v1.1 MD16G1076400.v1.1
prunus_persica Prupe.1G270600_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0440201
rosa_laevigata RLG00000006196
rosa_multiflora Rmu_sc0001685.1_g000028
rosa_roxburghii Rroxscaffold_5G00381130
rosa_rugosa Rorug04G0325200 Rorug04G0325300.1
rosa_samantha Rh4AG376000 Rh4DG382800
rosa_wichuraiana Rw4G032180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 794
AccB1I GGYRCC 3 cut(s) 658, 794, 1177
AccB7I CCANNNNNTGG 1 cut(s) 565
AccI GTMKAC 2 cut(s) 318, 858
AccII CGCG 2 cut(s) 501, 654
AciI CCGC 4 cut(s) 303, 357, 499, 654
AcsI RAATTY 3 cut(s) 46, 181, 446
AcyI GRCGYC 1 cut(s) 659
AfaI GTAC 4 cut(s) 494, 533, 796, 821
AfiI CCNNNNNNNGG 2 cut(s) 371, 565
AgsI TTSAA 5 cut(s) 38, 256, 690, 715, 1074
AjnI CCWGG 3 cut(s) 738, 765, 1180
AloI GAACNNNNNNTCC 2 cut(s) 1041, 1073
AluBI AGCT 8 cut(s) 107, 260, 549, 686, 724, 864, 1034, 1112
AluI AGCT 8 cut(s) 107, 260, 549, 686, 724, 864, 1034, 1112
Alw21I GWGCWC 2 cut(s) 513, 879
Alw26I GTCTC 2 cut(s) 61, 997
AoxI GGCC 4 cut(s) 141, 555, 736, 1159
ApeKI GCWGC 3 cut(s) 279, 353, 1143
ApoI RAATTY 3 cut(s) 46, 181, 446
Asp718I GGTACC 1 cut(s) 794
AspLEI GCGC 2 cut(s) 661, 905
AspS9I GGNCC 4 cut(s) 141, 556, 752, 769
AsuC2I CCSGG 1 cut(s) 1022
AsuHPI GGTGA 4 cut(s) 21, 323, 518, 947
AvaII GGWCC 2 cut(s) 752, 769
BanI GGYRCC 3 cut(s) 658, 794, 1177
BbsI GAAGAC 1 cut(s) 635
Bbv12I GWGCWC 2 cut(s) 513, 879
BbvI GCAGC 3 cut(s) 291, 340, 1155
BccI CCATC 2 cut(s) 559, 917
BceAI ACGGC 1 cut(s) 890
BcgI CGANNNNNNTGC 1 cut(s) 1159
BciT130I CCWGG 3 cut(s) 740, 767, 1182
BclI TGATCA 2 cut(s) 3, 997
BcnI CCSGG 1 cut(s) 1022
BcoDI GTCTC 2 cut(s) 61, 997
BfoI RGCGCY 1 cut(s) 662
BglII AGATCT 1 cut(s) 885
BisI GCNGC 5 cut(s) 280, 354, 357, 499, 1144
BlsI GCNGC 5 cut(s) 281, 355, 358, 500, 1145
Bme1390I CCNGG 4 cut(s) 740, 767, 1022, 1182
Bme18I GGWCC 2 cut(s) 752, 769
BmgT120I GGNCC 4 cut(s) 141, 556, 752, 769
BmiI GGNNCC 5 cut(s) 660, 754, 796, 898, 1179
BmrFI CCNGG 4 cut(s) 740, 767, 1022, 1182
BmsI GCATC 2 cut(s) 994, 1052
BoxI GACNNNNGTC 1 cut(s) 1103
BpiI GAAGAC 1 cut(s) 635
BpmI CTGGAG 1 cut(s) 613
BpuMI CCSGG 1 cut(s) 1022
BsaHI GRCGYC 1 cut(s) 659
BsaI GGTCTC 1 cut(s) 61
BsaJI CCNNGG 1 cut(s) 627
BsaXI ACNNNNNCTCC 2 cut(s) 1041, 1071
Bsc4I CCNNNNNNNGG 2 cut(s) 371, 565
Bse118I RCCGGY 1 cut(s) 972
Bse1I ACTGG 2 cut(s) 596, 937
BseBI CCWGG 3 cut(s) 740, 767, 1182
BseDI CCNNGG 1 cut(s) 627
BseGI GGATG 5 cut(s) 466, 709, 928, 933, 1053
BseLI CCNNNNNNNGG 2 cut(s) 371, 565
BseMII CTCAG 4 cut(s) 72, 412, 807, 1092
BseNI ACTGG 2 cut(s) 596, 937
BseRI GAGGAG 2 cut(s) 610, 1136
BseXI GCAGC 3 cut(s) 291, 340, 1155
BsgI GTGCAG 1 cut(s) 132
Bsh1236I CGCG 2 cut(s) 501, 654
BshFI GGCC 4 cut(s) 143, 557, 738, 1161
BshNI GGYRCC 3 cut(s) 658, 794, 1177
BsiHKAI GWGCWC 2 cut(s) 513, 879
BsiSI CCGG 2 cut(s) 973, 1022
BslI CCNNNNNNNGG 2 cut(s) 371, 565
BsmAI GTCTC 2 cut(s) 61, 997
BsmI GAATGC 1 cut(s) 355
BsnI GGCC 4 cut(s) 143, 557, 738, 1161
Bso31I GGTCTC 1 cut(s) 61
Bsp1286I GDGCHC 2 cut(s) 513, 879
Bsp143I GATC 7 cut(s) 3, 26, 291, 676, 885, 937, 997
BspACI CCGC 4 cut(s) 303, 357, 499, 654
BspANI GGCC 4 cut(s) 143, 557, 738, 1161
BspCNI CTCAG 4 cut(s) 71, 411, 808, 1093
BspFNI CGCG 2 cut(s) 501, 654
BspLI GGNNCC 5 cut(s) 660, 754, 796, 898, 1179
BspT107I GGYRCC 3 cut(s) 658, 794, 1177
BspTNI GGTCTC 1 cut(s) 61
BsrFI RCCGGY 1 cut(s) 972
BsrI ACTGG 2 cut(s) 596, 937
BssAI RCCGGY 1 cut(s) 972
BssECI CCNNGG 1 cut(s) 627
BssMI GATC 7 cut(s) 3, 26, 291, 676, 885, 937, 997
BssNAI GTATAC 1 cut(s) 859
BssNI GRCGYC 1 cut(s) 659
BssT1I CCWWGG 1 cut(s) 627
Bst1107I GTATAC 1 cut(s) 859
Bst2UI CCWGG 3 cut(s) 740, 767, 1182
Bst4CI ACNGT 3 cut(s) 248, 585, 960
BstACI GRCGYC 1 cut(s) 659
BstC8I GCNNGC 2 cut(s) 82, 86
BstDEI CTNAG 7 cut(s) 58, 398, 461, 816, 880, 1080, 1101
BstF5I GGATG 5 cut(s) 466, 709, 928, 933, 1053
BstFNI CGCG 2 cut(s) 501, 654
BstH2I RGCGCY 1 cut(s) 662
BstHHI GCGC 2 cut(s) 661, 905
BstKTI GATC 7 cut(s) 6, 29, 294, 679, 888, 940, 1000
BstMAI GTCTC 2 cut(s) 61, 997
BstMBI GATC 7 cut(s) 3, 26, 291, 676, 885, 937, 997
BstMWI GCNNNNNNNGC 4 cut(s) 257, 276, 575, 982
BstNI CCWGG 3 cut(s) 740, 767, 1182
BstNSI RCATGY 4 cut(s) 84, 88, 104, 839
BstPAI GACNNNNGTC 1 cut(s) 1103
BstSCI CCNGG 4 cut(s) 738, 765, 1020, 1180
BstUI CGCG 2 cut(s) 501, 654
BstV1I GCAGC 3 cut(s) 291, 340, 1155
BstV2I GAAGAC 1 cut(s) 635
BstX2I RGATCY 1 cut(s) 885
BstXI CCANNNNNNTGG 1 cut(s) 177
BstYI RGATCY 1 cut(s) 885
BstZ17I GTATAC 1 cut(s) 859
BsuRI GGCC 4 cut(s) 143, 557, 738, 1161
BtgZI GCGATG 2 cut(s) 435, 793
BtsCI GGATG 5 cut(s) 466, 709, 928, 933, 1053
BtsIMutI CAGTG 2 cut(s) 253, 589
Cac8I GCNNGC 2 cut(s) 82, 86
CfoI GCGC 2 cut(s) 661, 905
Cfr10I RCCGGY 1 cut(s) 972
Cfr13I GGNCC 4 cut(s) 141, 556, 752, 769
Csp6I GTAC 4 cut(s) 493, 532, 795, 820
CviAII CATG 7 cut(s) 81, 85, 101, 535, 836, 1130, 1163
CviQI GTAC 4 cut(s) 493, 532, 795, 820
DdeI CTNAG 7 cut(s) 58, 398, 461, 816, 880, 1080, 1101
DinI GGCGCC 1 cut(s) 660
DpnI GATC 7 cut(s) 5, 28, 293, 678, 887, 939, 999
DpnII GATC 7 cut(s) 3, 26, 291, 676, 885, 937, 997
EciI GGCGGA 1 cut(s) 318
Eco130I CCWWGG 1 cut(s) 627
Eco31I GGTCTC 1 cut(s) 61
Eco47I GGWCC 2 cut(s) 752, 769
EcoRII CCWGG 3 cut(s) 738, 765, 1180
EcoT14I CCWWGG 1 cut(s) 627
EcoT22I ATGCAT 1 cut(s) 86
EgeI GGCGCC 1 cut(s) 660
EheI GGCGCC 1 cut(s) 660
ErhI CCWWGG 1 cut(s) 627
FaeI CATG 7 cut(s) 84, 88, 104, 538, 839, 1133, 1166
FatI CATG 7 cut(s) 80, 84, 100, 534, 835, 1129, 1162
FauI CCCGC 1 cut(s) 647
FbaI TGATCA 2 cut(s) 3, 997
FblI GTMKAC 2 cut(s) 318, 858
Fnu4HI GCNGC 5 cut(s) 280, 354, 357, 499, 1144
FokI GGATG 5 cut(s) 453, 716, 935, 940, 1040
Fsp4HI GCNGC 5 cut(s) 280, 354, 357, 499, 1144
GlaI GCGC 2 cut(s) 660, 904
GluI GCNGC 5 cut(s) 280, 354, 357, 499, 1144
GsuI CTGGAG 1 cut(s) 613
HaeII RGCGCY 1 cut(s) 662
HaeIII GGCC 4 cut(s) 143, 557, 738, 1161
HapII CCGG 2 cut(s) 973, 1022
HhaI GCGC 2 cut(s) 661, 905
Hin1I GRCGYC 1 cut(s) 659
Hin1II CATG 7 cut(s) 84, 88, 104, 538, 839, 1133, 1166
Hin6I GCGC 2 cut(s) 659, 903
HinP1I GCGC 2 cut(s) 659, 903
HindIII AAGCTT 3 cut(s) 258, 547, 684
HinfI GANTC 3 cut(s) 166, 306, 1070
HpaII CCGG 2 cut(s) 973, 1022
HphI GGTGA 4 cut(s) 21, 323, 518, 947
Hpy166II GTNNAC 4 cut(s) 11, 196, 319, 859
Hpy188I TCNGA 8 cut(s) 61, 165, 401, 730, 922, 1002, 1061, 1152
Hpy188III TCNNGA 2 cut(s) 38, 674
Hpy8I GTNNAC 4 cut(s) 11, 196, 319, 859
Hpy99I CGWCG 1 cut(s) 857
HpyAV CCTTC 2 cut(s) 196, 497
HpyCH4III ACNGT 3 cut(s) 248, 585, 960
HpyCH4IV ACGT 3 cut(s) 13, 124, 855
HpyCH4V TGCA 8 cut(s) 80, 84, 88, 113, 137, 637, 985, 1129
HpyF10VI GCNNNNNNNGC 4 cut(s) 257, 276, 575, 982
HpyF3I CTNAG 7 cut(s) 58, 398, 461, 816, 880, 1080, 1101
HpySE526I ACGT 3 cut(s) 13, 124, 855
Hsp92I GRCGYC 1 cut(s) 659
Hsp92II CATG 7 cut(s) 84, 88, 104, 538, 839, 1133, 1166
HspAI GCGC 2 cut(s) 659, 903
KasI GGCGCC 1 cut(s) 658
KpnI GGTACC 1 cut(s) 798
Ksp22I TGATCA 2 cut(s) 3, 997
Kzo9I GATC 7 cut(s) 3, 26, 291, 676, 885, 937, 997
LmnI GCTCC 1 cut(s) 902
Lsp1109I GCAGC 3 cut(s) 291, 340, 1155
LweI GCATC 2 cut(s) 994, 1052
MaeII ACGT 3 cut(s) 13, 124, 855
MaeIII GTNAC 4 cut(s) 242, 585, 838, 1165
MalI GATC 7 cut(s) 5, 28, 293, 678, 887, 939, 999
MboI GATC 7 cut(s) 3, 26, 291, 676, 885, 937, 997
MboII GAAGA 6 cut(s) 36, 612, 635, 922, 956, 1040
MfeI CAATTG 1 cut(s) 381
MflI RGATCY 1 cut(s) 885
MhlI GDGCHC 2 cut(s) 513, 879
MluCI AATT 6 cut(s) 46, 181, 381, 446, 608, 666
Mly113I GGCGCC 1 cut(s) 659
MlyI GAGTC 1 cut(s) 175
MmeI TCCRAC 1 cut(s) 681
Mph1103I ATGCAT 1 cut(s) 86
MslI CAYNNNNRTG 1 cut(s) 68
MspI CCGG 2 cut(s) 973, 1022
MspR9I CCNGG 4 cut(s) 740, 767, 1022, 1182
MunI CAATTG 1 cut(s) 381
Mva1269I GAATGC 1 cut(s) 355
MvaI CCWGG 3 cut(s) 740, 767, 1182
MvnI CGCG 2 cut(s) 501, 654
MwoI GCNNNNNNNGC 4 cut(s) 257, 276, 575, 982
NarI GGCGCC 1 cut(s) 659
NciI CCSGG 1 cut(s) 1022
NdeII GATC 7 cut(s) 3, 26, 291, 676, 885, 937, 997
NlaIII CATG 7 cut(s) 84, 88, 104, 538, 839, 1133, 1166
NlaIV GGNNCC 5 cut(s) 660, 754, 796, 898, 1179
NmeAIII GCCGAG 1 cut(s) 597
NsiI ATGCAT 1 cut(s) 86
NspI RCATGY 4 cut(s) 84, 88, 104, 839
PaeI GCATGC 2 cut(s) 84, 88
PcsI WCGNNNNNNNCGW 1 cut(s) 19
PctI GAATGC 1 cut(s) 355
PfeI GAWTC 2 cut(s) 306, 1070
PflMI CCANNNNNTGG 1 cut(s) 565
PkrI GCNGC 5 cut(s) 281, 355, 358, 500, 1145
PleI GAGTC 1 cut(s) 174
PluTI GGCGCC 1 cut(s) 662
PpsI GAGTC 1 cut(s) 174
PshAI GACNNNNGTC 1 cut(s) 1103
Psp6I CCWGG 3 cut(s) 738, 765, 1180
PspGI CCWGG 3 cut(s) 738, 765, 1180
PspN4I GGNNCC 5 cut(s) 660, 754, 796, 898, 1179
PspPI GGNCC 4 cut(s) 141, 556, 752, 769
PsuI RGATCY 1 cut(s) 885
RsaI GTAC 4 cut(s) 494, 533, 796, 821
RsaNI GTAC 4 cut(s) 493, 532, 795, 820
RseI CAYNNNNRTG 1 cut(s) 68
SatI GCNGC 5 cut(s) 280, 354, 357, 499, 1144
Sau3AI GATC 7 cut(s) 3, 26, 291, 676, 885, 937, 997
Sau96I GGNCC 4 cut(s) 141, 556, 752, 769
SchI GAGTC 1 cut(s) 175
ScrFI CCNGG 4 cut(s) 740, 767, 1022, 1182
SduI GDGCHC 2 cut(s) 513, 879
SfaNI GCATC 2 cut(s) 994, 1052
SfoI GGCGCC 1 cut(s) 660
SinI GGWCC 2 cut(s) 752, 769
SmiMI CAYNNNNRTG 1 cut(s) 68
SphI GCATGC 2 cut(s) 84, 88
Sse9I AATT 6 cut(s) 46, 181, 381, 446, 608, 666
SsiI CCGC 4 cut(s) 303, 357, 499, 654
SspDI GGCGCC 1 cut(s) 658
SspI AATATT 1 cut(s) 917
StyD4I CCNGG 4 cut(s) 738, 765, 1020, 1180
StyI CCWWGG 1 cut(s) 627
TaaI ACNGT 3 cut(s) 248, 585, 960
TaiI ACGT 3 cut(s) 16, 127, 858
TaqI TCGA 1 cut(s) 1068
TaqII GACCGA 1 cut(s) 43
TasI AATT 6 cut(s) 46, 181, 381, 446, 608, 666
TatI WGTACW 2 cut(s) 531, 819
TauI GCSGC 2 cut(s) 359, 501
TfiI GAWTC 2 cut(s) 306, 1070
TscAI CASTG 2 cut(s) 253, 596
TseI GCWGC 3 cut(s) 279, 353, 1143
TspDTI ATGAA 4 cut(s) 438, 551, 659, 1146
TspGWI ACGGA 1 cut(s) 222
TspRI CASTG 2 cut(s) 253, 596
Van91I CCANNNNNTGG 1 cut(s) 565
VpaK11BI GGWCC 2 cut(s) 752, 769
XapI RAATTY 3 cut(s) 46, 181, 446
XceI RCATGY 4 cut(s) 84, 88, 104, 839
XmiI GTMKAC 2 cut(s) 318, 858
Zsp2I ATGCAT 1 cut(s) 86
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.