FvH4_4g33760

Peroxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0011412
Physical Location & Seq
Forward (+)
31859145 .. 31862765
3621 bp
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UTR
Exon/CDS
Intron
FvH4_4g33760.t1

Sequence Viewer

Length: 618 bp
ATGGCTTCCTCTCCTGTATCAGATACCAACAATAAGCAAGAAGTTGGTGGGAGATTTGTTCCGAAAGAACACGACTCTTTGCAGAGACATGTAGCATTTTTCGACAGGAATCACGATGGCATTGTTTATCCTTGGGAGACCTTTCAAGGTTTTCGTGCAATTGGGTGTGGCATCCTCTTGTCCTCTTTCAGTGCTATCTTCATCAACTTCGGTCTCAGTCGACAGACGCGCCCGGGGAAGTCGTTTTCTCTGCTGTTTCCGATTGAAGTTAGAAACATTCACAAAGCCAAACATGGCAGTGACTCAGGTGTCTACGACAGTGAAGGAAGGTTCGTTCCCTCAAAATTTGAAGAGATTTTCATCAAGCATGCACACACTAATGGCAATGCTTTGACATCAGAAGAACTAGATGGTTTGCTCAAAGCCAACAGGGAACCAAATGATCGCAGAGGATGGTTGGCGGCCTGGACTGAATGGAAGATCCTGTATTTGCTTTGCAAGGACAAGAATGGTTTACTGCACAAAGACACCATTAGAGCTGTCTACGATGGGAGCTTGTTCGAGCGTATGGAGAAGGAAAGATCAGAAGCAGCAGCCAAGAAGAAAGATATTGCCTGA

Protein Analysis

206

Amino Acids

23.27

Weight (kDa)

8.82

Isoelectric Point (pI)

45.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Caleosin PF05042 24 - 190 1.6e-72 Caleosin related protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011412)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G70670
fragaria_vesca FvH4_4g33760 FvH4_4g33760
malus_domestica MD13G1029500.v1.1 MD16G1020300.v1.1
prunus_persica Prupe.1G332000_v2.0.a1 Prupe.1G332000_v2.0.a1 Prupe.1G332000_v2.0.a1
pyrus_communis pycom13g01800 pycom16g01820
rosa_chinensis RchiOBHm_Chr4g0443291
rosa_laevigata RLG00000005940
rosa_multiflora Rmu_ssc0000122.1_g000004
rosa_roxburghii Rroxscaffold_5G00383880
rosa_rugosa Rorug04G0345400
rosa_samantha Rh4AG400300 Rh4BG411900 Rh4CG427200 Rh4DG407200
rosa_wichuraiana Rw4G034530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 308
AccI GTMKAC 3 cut(s) 220, 312, 543
AccII CGCG 1 cut(s) 229
AciI CCGC 1 cut(s) 461
AclWI GGATC 1 cut(s) 475
AcsI RAATTY 1 cut(s) 344
AflIII ACRYGT 1 cut(s) 88
AgsI TTSAA 3 cut(s) 146, 266, 350
AjnI CCWGG 1 cut(s) 464
AloI GAACNNNNNNTCC 2 cut(s) 318, 350
AluBI AGCT 2 cut(s) 539, 555
AluI AGCT 2 cut(s) 539, 555
Alw26I GTCTC 3 cut(s) 79, 131, 218
AlwI GGATC 1 cut(s) 475
Ama87I CYCGRG 1 cut(s) 232
AoxI GGCC 1 cut(s) 462
ApeKI GCWGC 2 cut(s) 590, 593
ApoI RAATTY 1 cut(s) 344
AspLEI GCGC 1 cut(s) 231
AsuC2I CCSGG 2 cut(s) 233, 234
AvaI CYCGRG 1 cut(s) 232
BbvI GCAGC 2 cut(s) 602, 605
BccI CCATC 4 cut(s) 110, 404, 447, 542
BciT130I CCWGG 1 cut(s) 466
BcnI CCSGG 2 cut(s) 233, 234
BcoDI GTCTC 3 cut(s) 79, 131, 218
BfaI CTAG 1 cut(s) 407
BisI GCNGC 3 cut(s) 462, 591, 594
BlsI GCNGC 3 cut(s) 463, 592, 595
Bme1390I CCNGG 3 cut(s) 233, 234, 466
BmeT110I CYCGRG 1 cut(s) 232
BmiI GGNNCC 1 cut(s) 435
BmrFI CCNGG 3 cut(s) 233, 234, 466
BmsI GCATC 1 cut(s) 180
BpuMI CCSGG 2 cut(s) 233, 234
BsaBI GATNNNNATC 1 cut(s) 359
BsaI GGTCTC 2 cut(s) 131, 218
BsaJI CCNNGG 3 cut(s) 131, 232, 233
Bse3DI GCAATG 1 cut(s) 391
Bse8I GATNNNNATC 1 cut(s) 359
BseBI CCWGG 1 cut(s) 466
BseDI CCNNGG 3 cut(s) 131, 232, 233
BseGI GGATG 2 cut(s) 171, 458
BseJI GATNNNNATC 1 cut(s) 359
BseMI GCAATG 1 cut(s) 391
BseMII CTCAG 2 cut(s) 229, 318
BseXI GCAGC 2 cut(s) 602, 605
BsgI GTGCAG 1 cut(s) 503
Bsh1236I CGCG 1 cut(s) 229
BshFI GGCC 1 cut(s) 464
BsiHKCI CYCGRG 1 cut(s) 232
BsiSI CCGG 1 cut(s) 233
BsmAI GTCTC 3 cut(s) 79, 131, 218
BsnI GGCC 1 cut(s) 464
Bso31I GGTCTC 2 cut(s) 131, 218
BsoBI CYCGRG 1 cut(s) 232
Bsp143I GATC 3 cut(s) 442, 480, 581
BspACI CCGC 1 cut(s) 461
BspANI GGCC 1 cut(s) 464
BspCNI CTCAG 2 cut(s) 228, 317
BspFNI CGCG 1 cut(s) 229
BspLI GGNNCC 1 cut(s) 435
BspPI GGATC 1 cut(s) 475
BspTNI GGTCTC 2 cut(s) 131, 218
BsrDI GCAATG 1 cut(s) 391
BssECI CCNNGG 3 cut(s) 131, 232, 233
BssMI GATC 3 cut(s) 442, 480, 581
BssT1I CCWWGG 1 cut(s) 131
Bst2UI CCWGG 1 cut(s) 466
Bst4CI ACNGT 1 cut(s) 320
Bst6I CTCTTC 1 cut(s) 345
BstC8I GCNNGC 1 cut(s) 369
BstDEI CTNAG 2 cut(s) 215, 304
BstF5I GGATG 2 cut(s) 171, 458
BstFNI CGCG 1 cut(s) 229
BstHHI GCGC 1 cut(s) 231
BstKTI GATC 3 cut(s) 445, 483, 584
BstMAI GTCTC 3 cut(s) 79, 131, 218
BstMBI GATC 3 cut(s) 442, 480, 581
BstNI CCWGG 1 cut(s) 466
BstNSI RCATGY 2 cut(s) 92, 371
BstSCI CCNGG 3 cut(s) 231, 232, 464
BstUI CGCG 1 cut(s) 229
BstV1I GCAGC 2 cut(s) 602, 605
BstX2I RGATCY 1 cut(s) 480
BstYI RGATCY 1 cut(s) 480
BsuRI GGCC 1 cut(s) 464
BtsCI GGATG 2 cut(s) 171, 458
BtsI GCAGTG 1 cut(s) 304
BtsIMutI CAGTG 3 cut(s) 196, 304, 325
Cac8I GCNNGC 1 cut(s) 369
CfoI GCGC 1 cut(s) 231
Cfr9I CCCGGG 1 cut(s) 232
CseI GACGC 1 cut(s) 235
CviAII CATG 3 cut(s) 89, 293, 368
CviJI RGCY 7 cut(s) 5, 287, 425, 464, 539, 555, 596
CviKI_1 RGCY 7 cut(s) 5, 287, 425, 464, 539, 555, 596
DdeI CTNAG 2 cut(s) 215, 304
DpnI GATC 3 cut(s) 444, 482, 583
DpnII GATC 3 cut(s) 442, 480, 581
DrdI GACNNNNNNGTC 1 cut(s) 308
DseDI GACNNNNNNGTC 1 cut(s) 308
Eam1104I CTCTTC 1 cut(s) 345
EarI CTCTTC 1 cut(s) 345
Eco130I CCWWGG 1 cut(s) 131
Eco31I GGTCTC 2 cut(s) 131, 218
Eco88I CYCGRG 1 cut(s) 232
EcoRII CCWGG 1 cut(s) 464
EcoT14I CCWWGG 1 cut(s) 131
ErhI CCWWGG 1 cut(s) 131
FaeI CATG 3 cut(s) 92, 296, 371
FaiI YATR 4 cut(s) 90, 294, 369, 569
FatI CATG 3 cut(s) 88, 292, 367
FblI GTMKAC 3 cut(s) 220, 312, 543
Fnu4HI GCNGC 3 cut(s) 462, 591, 594
FokI GGATG 2 cut(s) 158, 465
Fsp4HI GCNGC 3 cut(s) 462, 591, 594
FspBI CTAG 1 cut(s) 407
GlaI GCGC 1 cut(s) 230
GluI GCNGC 3 cut(s) 462, 591, 594
HaeIII GGCC 1 cut(s) 464
HapII CCGG 1 cut(s) 233
HgaI GACGC 1 cut(s) 235
HhaI GCGC 1 cut(s) 231
Hin1II CATG 3 cut(s) 92, 296, 371
Hin6I GCGC 1 cut(s) 229
HinP1I GCGC 1 cut(s) 229
HincII GTYRAC 1 cut(s) 221
HindII GTYRAC 1 cut(s) 221
HinfI GANTC 3 cut(s) 74, 109, 302
HpaII CCGG 1 cut(s) 233
Hpy166II GTNNAC 4 cut(s) 221, 313, 515, 544
Hpy188I TCNGA 5 cut(s) 22, 63, 261, 400, 586
Hpy188III TCNNGA 1 cut(s) 113
Hpy8I GTNNAC 4 cut(s) 221, 313, 515, 544
HpyAV CCTTC 3 cut(s) 317, 321, 568
HpyCH4III ACNGT 1 cut(s) 320
HpyCH4V TGCA 5 cut(s) 82, 158, 371, 498, 520
HpyF3I CTNAG 2 cut(s) 215, 304
Hsp92II CATG 3 cut(s) 92, 296, 371
HspAI GCGC 1 cut(s) 229
Kzo9I GATC 3 cut(s) 442, 480, 581
LmnI GCTCC 1 cut(s) 552
LpnPI CCDG 8 cut(s) 27, 91, 246, 291, 415, 451, 478, 497
Lsp1109I GCAGC 2 cut(s) 602, 605
LweI GCATC 1 cut(s) 180
MaeI CTAG 1 cut(s) 407
MaeIII GTNAC 1 cut(s) 299
MalI GATC 3 cut(s) 444, 482, 583
MboI GATC 3 cut(s) 442, 480, 581
MboII GAAGA 5 cut(s) 190, 362, 413, 490, 613
MfeI CAATTG 1 cut(s) 159
MflI RGATCY 1 cut(s) 480
MluCI AATT 2 cut(s) 159, 344
MlyI GAGTC 2 cut(s) 68, 296
MnlI CCTC 5 cut(s) 19, 185, 193, 349, 443
MslI CAYNNNNRTG 2 cut(s) 297, 378
MspI CCGG 1 cut(s) 233
MspR9I CCNGG 3 cut(s) 233, 234, 466
MunI CAATTG 1 cut(s) 159
MvaI CCWGG 1 cut(s) 466
MvnI CGCG 1 cut(s) 229
NciI CCSGG 2 cut(s) 233, 234
NdeII GATC 3 cut(s) 442, 480, 581
NlaIII CATG 3 cut(s) 92, 296, 371
NlaIV GGNNCC 1 cut(s) 435
NmuCI GTSAC 1 cut(s) 299
NspI RCATGY 2 cut(s) 92, 371
PaeI GCATGC 1 cut(s) 371
PciI ACATGT 1 cut(s) 88
PfeI GAWTC 1 cut(s) 109
PkrI GCNGC 3 cut(s) 463, 592, 595
PleI GAGTC 2 cut(s) 68, 296
PpsI GAGTC 2 cut(s) 68, 296
PscI ACATGT 1 cut(s) 88
Psp6I CCWGG 1 cut(s) 464
PspGI CCWGG 1 cut(s) 464
PspN4I GGNNCC 1 cut(s) 435
PsuI RGATCY 1 cut(s) 480
RseI CAYNNNNRTG 2 cut(s) 297, 378
SalI GTCGAC 1 cut(s) 219
SatI GCNGC 3 cut(s) 462, 591, 594
Sau3AI GATC 3 cut(s) 442, 480, 581
SchI GAGTC 2 cut(s) 68, 296
ScrFI CCNGG 3 cut(s) 233, 234, 466
SetI ASST 6 cut(s) 143, 151, 310, 332, 541, 557
SfaNI GCATC 1 cut(s) 180
SmaI CCCGGG 1 cut(s) 234
SmiMI CAYNNNNRTG 2 cut(s) 297, 378
SphI GCATGC 1 cut(s) 371
Sse9I AATT 2 cut(s) 159, 344
SsiI CCGC 1 cut(s) 461
SspMI CTAG 1 cut(s) 407
StyD4I CCNGG 3 cut(s) 231, 232, 464
StyI CCWWGG 1 cut(s) 131
TaaI ACNGT 1 cut(s) 320
TaqI TCGA 3 cut(s) 102, 220, 561
TaqII GACCGA 1 cut(s) 200
TasI AATT 2 cut(s) 159, 344
TauI GCSGC 1 cut(s) 464
TfiI GAWTC 1 cut(s) 109
TscAI CASTG 3 cut(s) 196, 304, 325
TseFI GTSAC 1 cut(s) 299
TseI GCWGC 2 cut(s) 590, 593
Tsp45I GTSAC 1 cut(s) 299
TspDTI ATGAA 2 cut(s) 190, 349
TspMI CCCGGG 1 cut(s) 232
TspRI CASTG 3 cut(s) 196, 304, 325
XapI RAATTY 1 cut(s) 344
XceI RCATGY 2 cut(s) 92, 371
XmaI CCCGGG 1 cut(s) 232
XmiI GTMKAC 3 cut(s) 220, 312, 543
XspI CTAG 1 cut(s) 407
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.