Rw4G034530

Peroxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0011412
Physical Location & Seq
Forward (+)
61574143 .. 61578203
4061 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G034530.1

Sequence Viewer

Length: 615 bp
ATGTGTAATGGGATTGAAACTTACAGCAAATCTAATCAAGTTAGTGGGAAATTTGTTCCGAATGAACACGATGCTCTGCAGAGGCATGTAGCATTCTTCGACAGGAATCACGATGGCATTGTTTATCCTTGGGAGACCTTTCAAGGTTTTCGTGCAATTGGGTGTGGCCTCCTCTTGTCCTCGTTCAGTGCTATCTTCATCAACTTCGGTCTCAGTCGGCAGACTCGCCCGGGAAAGTCGTTTTCTCTGCTGTTTCCGATTGAAGTTAGAAATATTCACAAAGCCAAACACGGCAGTGACTCAGGCGTCTACGACAATGAAGGAAGGTTTGTTCCCTGTAAGTTTGAAGAGATTTTCATCAAGCATGCACATACTAATCCCAATGCTTTGACATCTGCCGAACTGAATGGGCTGCTCAAAGCAAATAGGGAACCAAATGATCGCAGAGGATGGTTGGCTGCCTGGACTGAATGGAAGATCCTGTATTTGCTTTGCAAGGACAAGCATGGTTTACTGCACAAAGAAACCATTAGAGCTGTTTATGATGGGAGCTTGTTCGACCGTATGGAGAAGGAAAGATCAGCTGCAGCAGCCAAGAAGAAAGATATTGTCTGA

Protein Analysis

204

Amino Acids

23.23

Weight (kDa)

9.16

Isoelectric Point (pI)

32.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Caleosin PF05042 23 - 189 2.9e-72 Caleosin related protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011412)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G70670
fragaria_vesca FvH4_4g33760 FvH4_4g33760
malus_domestica MD13G1029500.v1.1 MD16G1020300.v1.1
prunus_persica Prupe.1G332000_v2.0.a1 Prupe.1G332000_v2.0.a1 Prupe.1G332000_v2.0.a1
pyrus_communis pycom13g01800 pycom16g01820
rosa_chinensis RchiOBHm_Chr4g0443291
rosa_laevigata RLG00000005940
rosa_multiflora Rmu_ssc0000122.1_g000004
rosa_roxburghii Rroxscaffold_5G00383880
rosa_rugosa Rorug04G0345400
rosa_samantha Rh4AG400300 Rh4BG411900 Rh4CG427200 Rh4DG407200
rosa_wichuraiana Rw4G034530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 305
AccI GTMKAC 1 cut(s) 309
AclWI GGATC 1 cut(s) 472
AcsI RAATTY 1 cut(s) 50
AcyI GRCGYC 1 cut(s) 306
AgsI TTSAA 4 cut(s) 17, 143, 263, 347
AjnI CCWGG 1 cut(s) 461
AleI CACNNNNGTG 1 cut(s) 294
AloI GAACNNNNNNTCC 2 cut(s) 315, 347
AluBI AGCT 3 cut(s) 536, 552, 584
AluI AGCT 3 cut(s) 536, 552, 584
Alw26I GTCTC 2 cut(s) 128, 215
AlwI GGATC 1 cut(s) 472
Ama87I CYCGRG 1 cut(s) 229
AoxI GGCC 1 cut(s) 166
ApeKI GCWGC 5 cut(s) 412, 458, 584, 587, 590
ApoI RAATTY 1 cut(s) 50
AsuC2I CCSGG 2 cut(s) 230, 231
AvaI CYCGRG 1 cut(s) 229
BbvI GCAGC 5 cut(s) 399, 445, 571, 599, 602
BccI CCATC 3 cut(s) 107, 444, 539
BceAI ACGGC 1 cut(s) 307
BciT130I CCWGG 1 cut(s) 463
BcnI CCSGG 2 cut(s) 230, 231
BcoDI GTCTC 2 cut(s) 128, 215
BfmI CTRYAG 2 cut(s) 77, 585
BisI GCNGC 5 cut(s) 413, 459, 585, 588, 591
BlsI GCNGC 5 cut(s) 414, 460, 586, 589, 592
Bme1390I CCNGG 3 cut(s) 230, 231, 463
BmeT110I CYCGRG 1 cut(s) 229
BmiI GGNNCC 1 cut(s) 432
BmrFI CCNGG 3 cut(s) 230, 231, 463
BmsI GCATC 1 cut(s) 61
BpuMI CCSGG 2 cut(s) 230, 231
BsaBI GATNNNNATC 1 cut(s) 356
BsaHI GRCGYC 1 cut(s) 306
BsaI GGTCTC 2 cut(s) 128, 215
BsaJI CCNNGG 2 cut(s) 128, 229
Bse8I GATNNNNATC 1 cut(s) 356
BseBI CCWGG 1 cut(s) 463
BseDI CCNNGG 2 cut(s) 128, 229
BseGI GGATG 1 cut(s) 455
BseJI GATNNNNATC 1 cut(s) 356
BseMII CTCAG 2 cut(s) 226, 315
BseRI GAGGAG 1 cut(s) 161
BseXI GCAGC 5 cut(s) 399, 445, 571, 599, 602
BsgI GTGCAG 1 cut(s) 500
Bsh1285I CGRYCG 1 cut(s) 562
BshFI GGCC 1 cut(s) 168
BsiEI CGRYCG 1 cut(s) 562
BsiHKCI CYCGRG 1 cut(s) 229
BsiSI CCGG 1 cut(s) 230
BsmAI GTCTC 2 cut(s) 128, 215
BsmI GAATGC 1 cut(s) 92
BsnI GGCC 1 cut(s) 168
Bso31I GGTCTC 2 cut(s) 128, 215
BsoBI CYCGRG 1 cut(s) 229
Bsp143I GATC 3 cut(s) 439, 477, 578
BspANI GGCC 1 cut(s) 168
BspCNI CTCAG 2 cut(s) 225, 314
BspLI GGNNCC 1 cut(s) 432
BspMAI CTGCAG 2 cut(s) 81, 589
BspPI GGATC 1 cut(s) 472
BspTNI GGTCTC 2 cut(s) 128, 215
BssECI CCNNGG 2 cut(s) 128, 229
BssMI GATC 3 cut(s) 439, 477, 578
BssNI GRCGYC 1 cut(s) 306
BssT1I CCWWGG 1 cut(s) 128
Bst2UI CCWGG 1 cut(s) 463
Bst4CI ACNGT 1 cut(s) 563
Bst6I CTCTTC 1 cut(s) 342
BstACI GRCGYC 1 cut(s) 306
BstC8I GCNNGC 1 cut(s) 366
BstDEI CTNAG 2 cut(s) 212, 301
BstF5I GGATG 1 cut(s) 455
BstKTI GATC 3 cut(s) 442, 480, 581
BstMAI GTCTC 2 cut(s) 128, 215
BstMBI GATC 3 cut(s) 439, 477, 578
BstMCI CGRYCG 1 cut(s) 562
BstMWI GCNNNNNNNGC 1 cut(s) 590
BstNI CCWGG 1 cut(s) 463
BstNSI RCATGY 2 cut(s) 89, 368
BstSCI CCNGG 3 cut(s) 228, 229, 461
BstSFI CTRYAG 2 cut(s) 77, 585
BstV1I GCAGC 5 cut(s) 399, 445, 571, 599, 602
BstX2I RGATCY 1 cut(s) 477
BstYI RGATCY 1 cut(s) 477
BsuRI GGCC 1 cut(s) 168
BtsCI GGATG 1 cut(s) 455
BtsI GCAGTG 1 cut(s) 301
BtsIMutI CAGTG 2 cut(s) 193, 301
Cac8I GCNNGC 1 cut(s) 366
Cfr9I CCCGGG 1 cut(s) 229
CseI GACGC 1 cut(s) 295
CviAII CATG 3 cut(s) 86, 365, 506
CviJI RGCY 8 cut(s) 168, 284, 412, 458, 536, 552, 584, 593
CviKI_1 RGCY 8 cut(s) 168, 284, 412, 458, 536, 552, 584, 593
DdeI CTNAG 2 cut(s) 212, 301
DpnI GATC 3 cut(s) 441, 479, 580
DpnII GATC 3 cut(s) 439, 477, 578
DrdI GACNNNNNNGTC 1 cut(s) 305
DseDI GACNNNNNNGTC 1 cut(s) 305
Eam1104I CTCTTC 1 cut(s) 342
EarI CTCTTC 1 cut(s) 342
Eco130I CCWWGG 1 cut(s) 128
Eco31I GGTCTC 2 cut(s) 128, 215
Eco88I CYCGRG 1 cut(s) 229
EcoRII CCWGG 1 cut(s) 461
EcoT14I CCWWGG 1 cut(s) 128
ErhI CCWWGG 1 cut(s) 128
FaeI CATG 3 cut(s) 89, 368, 509
FaiI YATR 6 cut(s) 87, 366, 372, 507, 543, 566
FatI CATG 3 cut(s) 85, 364, 505
FblI GTMKAC 1 cut(s) 309
Fnu4HI GCNGC 5 cut(s) 413, 459, 585, 588, 591
FokI GGATG 1 cut(s) 462
Fsp4HI GCNGC 5 cut(s) 413, 459, 585, 588, 591
GluI GCNGC 5 cut(s) 413, 459, 585, 588, 591
HaeIII GGCC 1 cut(s) 168
HapII CCGG 1 cut(s) 230
HgaI GACGC 1 cut(s) 295
Hin1I GRCGYC 1 cut(s) 306
Hin1II CATG 3 cut(s) 89, 368, 509
HinfI GANTC 3 cut(s) 106, 223, 299
HpaII CCGG 1 cut(s) 230
Hpy166II GTNNAC 2 cut(s) 310, 512
Hpy188I TCNGA 3 cut(s) 60, 258, 614
Hpy188III TCNNGA 1 cut(s) 110
Hpy8I GTNNAC 2 cut(s) 310, 512
HpyAV CCTTC 3 cut(s) 314, 318, 565
HpyCH4III ACNGT 1 cut(s) 563
HpyCH4V TGCA 6 cut(s) 79, 155, 368, 495, 517, 587
HpyF10VI GCNNNNNNNGC 1 cut(s) 590
HpyF3I CTNAG 2 cut(s) 212, 301
Hsp92I GRCGYC 1 cut(s) 306
Hsp92II CATG 3 cut(s) 89, 368, 509
Kzo9I GATC 3 cut(s) 439, 477, 578
LmnI GCTCC 1 cut(s) 549
LpnPI CCDG 7 cut(s) 88, 243, 288, 349, 448, 475, 494
Lsp1109I GCAGC 5 cut(s) 399, 445, 571, 599, 602
LweI GCATC 1 cut(s) 61
MaeIII GTNAC 1 cut(s) 296
MalI GATC 3 cut(s) 441, 479, 580
MboI GATC 3 cut(s) 439, 477, 578
MboII GAAGA 5 cut(s) 88, 187, 359, 487, 610
MfeI CAATTG 1 cut(s) 156
MflI RGATCY 1 cut(s) 477
MluCI AATT 2 cut(s) 50, 156
MlyI GAGTC 2 cut(s) 217, 293
MnlI CCTC 5 cut(s) 75, 179, 182, 190, 440
MslI CAYNNNNRTG 1 cut(s) 294
MspA1I CMGCKG 1 cut(s) 584
MspI CCGG 1 cut(s) 230
MspR9I CCNGG 3 cut(s) 230, 231, 463
MunI CAATTG 1 cut(s) 156
Mva1269I GAATGC 1 cut(s) 92
MvaI CCWGG 1 cut(s) 463
MwoI GCNNNNNNNGC 1 cut(s) 590
NciI CCSGG 2 cut(s) 230, 231
NdeII GATC 3 cut(s) 439, 477, 578
NlaIII CATG 3 cut(s) 89, 368, 509
NlaIV GGNNCC 1 cut(s) 432
NmuCI GTSAC 1 cut(s) 296
NspI RCATGY 2 cut(s) 89, 368
OliI CACNNNNGTG 1 cut(s) 294
PaeI GCATGC 1 cut(s) 368
PctI GAATGC 1 cut(s) 92
PfeI GAWTC 1 cut(s) 106
PkrI GCNGC 5 cut(s) 414, 460, 586, 589, 592
PleI GAGTC 2 cut(s) 217, 293
PpsI GAGTC 2 cut(s) 217, 293
Psp6I CCWGG 1 cut(s) 461
PspGI CCWGG 1 cut(s) 461
PspN4I GGNNCC 1 cut(s) 432
PstI CTGCAG 2 cut(s) 81, 589
PsuI RGATCY 1 cut(s) 477
PvuII CAGCTG 1 cut(s) 584
RseI CAYNNNNRTG 1 cut(s) 294
SatI GCNGC 5 cut(s) 413, 459, 585, 588, 591
Sau3AI GATC 3 cut(s) 439, 477, 578
SchI GAGTC 2 cut(s) 217, 293
ScrFI CCNGG 3 cut(s) 230, 231, 463
SetI ASST 6 cut(s) 140, 148, 329, 538, 554, 586
SfaNI GCATC 1 cut(s) 61
SfcI CTRYAG 2 cut(s) 77, 585
SmaI CCCGGG 1 cut(s) 231
SmiMI CAYNNNNRTG 1 cut(s) 294
SphI GCATGC 1 cut(s) 368
Sse9I AATT 2 cut(s) 50, 156
SspI AATATT 1 cut(s) 274
StyD4I CCNGG 3 cut(s) 228, 229, 461
StyI CCWWGG 1 cut(s) 128
TaaI ACNGT 1 cut(s) 563
TaqI TCGA 2 cut(s) 99, 558
TaqII GACCGA 1 cut(s) 197
TasI AATT 2 cut(s) 50, 156
TfiI GAWTC 1 cut(s) 106
TscAI CASTG 2 cut(s) 193, 301
TseFI GTSAC 1 cut(s) 296
TseI GCWGC 5 cut(s) 412, 458, 584, 587, 590
Tsp45I GTSAC 1 cut(s) 296
TspDTI ATGAA 4 cut(s) 78, 187, 333, 346
TspMI CCCGGG 1 cut(s) 229
TspRI CASTG 2 cut(s) 193, 301
XapI RAATTY 1 cut(s) 50
XceI RCATGY 2 cut(s) 89, 368
XmaI CCCGGG 1 cut(s) 229
XmiI GTMKAC 1 cut(s) 309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.