pycom16g01820

Peroxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0011412
Physical Location & Seq
Forward (+)
1118468 .. 1121518
3051 bp
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UTR
Exon/CDS
Intron
pycom16g01820.2

Sequence Viewer

Length: 612 bp
ATGGGTTCCTCTTCTCTGTCAGAAAACAGTAAGCAACAAGATAATAATGGTGGGAAATTCGTACCCAACAAAGAGGACGTTCTGCAGAGACATGTAGCCTTCTTTGACAGGAATCACGATGGCATTGTTTATCCTTGGGAAACTTTTCAAGGTTTTCGTGCGATCGGGTGTGGTATCATGTTGTCCACAGTCAGTGCCTTTCTCATCAACGCGGCCCTCAGTCGAAAGACTCGCCAGGGAAAGTTCCCTTCTCTGCTTTTCCCAGTTGAAGTTAAAAACATCCATAAAGCCAAACATGGCAGTGACTCAGGAGTCTATGATACTGAAGGAAGGTTTGTTCCGTCGAAATTTGAAGAAATCTTTGAGAAGCATGCCCATACGCATCCAAATGCGTTGACGTCAGATGAACTGATGGGCATGCTAAAGGCTAATAGGGATCCCAAGGATTATGCAGGATGGTTTGCTGCCTATACAGAATGGAAGATCTTATATGTGCTTTGCAAGGACAAAAATGGTTTGTTGCACAAGGATACAGTTAGAGCTGTTTACGATGGAAGCTTGTTTGAACGCATGGAGAAGGAAAACTCCGGCGGAGACAAAAAGAAAGAATAA

Protein Analysis

204

Amino Acids

22.96

Weight (kDa)

8.41

Isoelectric Point (pI)

37.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011412)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G70670
fragaria_vesca FvH4_4g33760 FvH4_4g33760
malus_domestica MD13G1029500.v1.1 MD16G1020300.v1.1
prunus_persica Prupe.1G332000_v2.0.a1 Prupe.1G332000_v2.0.a1 Prupe.1G332000_v2.0.a1
pyrus_communis pycom13g01800 pycom16g01820
rosa_chinensis RchiOBHm_Chr4g0443291
rosa_laevigata RLG00000005940
rosa_multiflora Rmu_ssc0000122.1_g000004
rosa_roxburghii Rroxscaffold_5G00383880
rosa_rugosa Rorug04G0345400
rosa_samantha Rh4AG400300 Rh4BG411900 Rh4CG427200 Rh4DG407200
rosa_wichuraiana Rw4G034530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 311
AatII GACGTC 1 cut(s) 401
AccII CGCG 1 cut(s) 212
AciI CCGC 2 cut(s) 212, 591
AclWI GGATC 2 cut(s) 431, 444
AcsI RAATTY 2 cut(s) 56, 347
AcuI CTGAAG 1 cut(s) 345
AcyI GRCGYC 1 cut(s) 398
AfaI GTAC 1 cut(s) 63
AflIII ACRYGT 1 cut(s) 91
AgsI TTSAA 4 cut(s) 149, 269, 353, 566
AjnI CCWGG 1 cut(s) 234
AloI GAACNNNNNNTCC 2 cut(s) 321, 353
AluBI AGCT 2 cut(s) 542, 558
AluI AGCT 2 cut(s) 542, 558
Alw26I GTCTC 2 cut(s) 82, 588
AlwI GGATC 2 cut(s) 431, 444
AoxI GGCC 1 cut(s) 213
ApeKI GCWGC 1 cut(s) 464
ApoI RAATTY 2 cut(s) 56, 347
Asp700I GAANNNNTTC 1 cut(s) 144
AspS9I GGNCC 1 cut(s) 214
BamHI GGATCC 1 cut(s) 436
BbvI GCAGC 1 cut(s) 451
BccI CCATC 4 cut(s) 113, 406, 450, 545
BciT130I CCWGG 1 cut(s) 236
BciVI GTATCC 1 cut(s) 523
BcoDI GTCTC 2 cut(s) 82, 588
BfmI CTRYAG 1 cut(s) 83
BfuI GTATCC 1 cut(s) 523
BglII AGATCT 1 cut(s) 483
BisI GCNGC 2 cut(s) 213, 465
BlsI GCNGC 2 cut(s) 214, 466
Bme1390I CCNGG 1 cut(s) 236
BmgT120I GGNCC 1 cut(s) 214
BmiI GGNNCC 2 cut(s) 7, 438
BmrFI CCNGG 1 cut(s) 236
BmrI ACTGGG 1 cut(s) 257
BmsI GCATC 1 cut(s) 391
BmuI ACTGGG 1 cut(s) 257
BsaHI GRCGYC 1 cut(s) 398
BsaJI CCNNGG 3 cut(s) 134, 235, 441
Bse1I ACTGG 1 cut(s) 263
BseBI CCWGG 1 cut(s) 236
BseDI CCNNGG 3 cut(s) 134, 235, 441
BseGI GGATG 3 cut(s) 279, 382, 461
BseMII CTCAG 2 cut(s) 232, 321
BseNI ACTGG 1 cut(s) 263
BseXI GCAGC 1 cut(s) 451
Bsh1236I CGCG 1 cut(s) 212
Bsh1285I CGRYCG 1 cut(s) 165
BshFI GGCC 1 cut(s) 215
BsiEI CGRYCG 1 cut(s) 165
BsiSI CCGG 1 cut(s) 588
BsmAI GTCTC 2 cut(s) 82, 588
BsnI GGCC 1 cut(s) 215
Bsp143I GATC 3 cut(s) 162, 436, 483
BspACI CCGC 2 cut(s) 212, 591
BspANI GGCC 1 cut(s) 215
BspCNI CTCAG 2 cut(s) 231, 320
BspFNI CGCG 1 cut(s) 212
BspLI GGNNCC 2 cut(s) 7, 438
BspMAI CTGCAG 1 cut(s) 87
BspPI GGATC 2 cut(s) 431, 444
BsrI ACTGG 1 cut(s) 263
BssECI CCNNGG 3 cut(s) 134, 235, 441
BssMI GATC 3 cut(s) 162, 436, 483
BssNI GRCGYC 1 cut(s) 398
BssT1I CCWWGG 2 cut(s) 134, 441
Bst2UI CCWGG 1 cut(s) 236
Bst4CI ACNGT 3 cut(s) 29, 190, 535
Bst6I CTCTTC 1 cut(s) 16
BstACI GRCGYC 1 cut(s) 398
BstC8I GCNNGC 2 cut(s) 372, 419
BstDEI CTNAG 2 cut(s) 218, 307
BstF5I GGATG 3 cut(s) 279, 382, 461
BstFNI CGCG 1 cut(s) 212
BstKTI GATC 3 cut(s) 165, 439, 486
BstMAI GTCTC 2 cut(s) 82, 588
BstMBI GATC 3 cut(s) 162, 436, 483
BstMCI CGRYCG 1 cut(s) 165
BstNI CCWGG 1 cut(s) 236
BstNSI RCATGY 3 cut(s) 95, 374, 421
BstSCI CCNGG 1 cut(s) 234
BstSFI CTRYAG 1 cut(s) 83
BstUI CGCG 1 cut(s) 212
BstV1I GCAGC 1 cut(s) 451
BstX2I RGATCY 2 cut(s) 436, 483
BstYI RGATCY 2 cut(s) 436, 483
BsuI GTATCC 1 cut(s) 523
BsuRI GGCC 1 cut(s) 215
BtsCI GGATG 3 cut(s) 279, 382, 461
BtsI GCAGTG 1 cut(s) 307
BtsIMutI CAGTG 2 cut(s) 199, 307
Cac8I GCNNGC 2 cut(s) 372, 419
Cfr13I GGNCC 1 cut(s) 214
Csp6I GTAC 1 cut(s) 62
CviAII CATG 6 cut(s) 92, 178, 296, 371, 418, 571
CviJI RGCY 6 cut(s) 98, 215, 290, 428, 542, 558
CviKI_1 RGCY 6 cut(s) 98, 215, 290, 428, 542, 558
CviQI GTAC 1 cut(s) 62
DdeI CTNAG 2 cut(s) 218, 307
DpnI GATC 3 cut(s) 164, 438, 485
DpnII GATC 3 cut(s) 162, 436, 483
DrdI GACNNNNNNGTC 1 cut(s) 311
DseDI GACNNNNNNGTC 1 cut(s) 311
Eam1104I CTCTTC 1 cut(s) 16
EarI CTCTTC 1 cut(s) 16
EciI GGCGGA 1 cut(s) 606
Eco130I CCWWGG 2 cut(s) 134, 441
Eco57I CTGAAG 1 cut(s) 345
EcoRII CCWGG 1 cut(s) 234
EcoT14I CCWWGG 2 cut(s) 134, 441
ErhI CCWWGG 2 cut(s) 134, 441
FaeI CATG 6 cut(s) 95, 181, 299, 374, 421, 574
FatI CATG 6 cut(s) 91, 177, 295, 370, 417, 570
Fnu4HI GCNGC 2 cut(s) 213, 465
FokI GGATG 3 cut(s) 266, 369, 468
Fsp4HI GCNGC 2 cut(s) 213, 465
GluI GCNGC 2 cut(s) 213, 465
HaeIII GGCC 1 cut(s) 215
HapII CCGG 1 cut(s) 588
Hin1I GRCGYC 1 cut(s) 398
Hin1II CATG 6 cut(s) 95, 181, 299, 374, 421, 574
HincII GTYRAC 1 cut(s) 396
HindII GTYRAC 1 cut(s) 396
HindIII AAGCTT 1 cut(s) 556
HinfI GANTC 4 cut(s) 112, 229, 305, 312
HpaII CCGG 1 cut(s) 588
Hpy166II GTNNAC 3 cut(s) 186, 396, 547
Hpy188I TCNGA 2 cut(s) 22, 403
Hpy188III TCNNGA 2 cut(s) 116, 309
Hpy8I GTNNAC 3 cut(s) 186, 396, 547
Hpy99I CGWCG 1 cut(s) 346
HpyAV CCTTC 5 cut(s) 109, 258, 320, 324, 571
HpyCH4III ACNGT 3 cut(s) 29, 190, 535
HpyCH4IV ACGT 2 cut(s) 78, 398
HpyCH4V TGCA 4 cut(s) 85, 452, 501, 523
HpyF3I CTNAG 2 cut(s) 218, 307
HpySE526I ACGT 2 cut(s) 78, 398
Hsp92I GRCGYC 1 cut(s) 398
Hsp92II CATG 6 cut(s) 95, 181, 299, 374, 421, 574
Kzo9I GATC 3 cut(s) 162, 436, 483
LpnPI CCDG 7 cut(s) 94, 221, 248, 276, 294, 438, 601
Lsp1109I GCAGC 1 cut(s) 451
LweI GCATC 1 cut(s) 391
MaeII ACGT 2 cut(s) 78, 398
MaeIII GTNAC 1 cut(s) 302
MalI GATC 3 cut(s) 164, 438, 485
MboI GATC 3 cut(s) 162, 436, 483
MboII GAAGA 3 cut(s) 3, 365, 493
MflI RGATCY 2 cut(s) 436, 483
MluCI AATT 2 cut(s) 56, 347
MlyI GAGTC 3 cut(s) 223, 299, 321
MnlI CCTC 3 cut(s) 19, 67, 227
MroXI GAANNNNTTC 1 cut(s) 144
MseI TTAA 1 cut(s) 273
MslI CAYNNNNRTG 2 cut(s) 300, 387
MspI CCGG 1 cut(s) 588
MspR9I CCNGG 1 cut(s) 236
MvaI CCWGG 1 cut(s) 236
MvnI CGCG 1 cut(s) 212
NdeII GATC 3 cut(s) 162, 436, 483
NlaIII CATG 6 cut(s) 95, 181, 299, 374, 421, 574
NlaIV GGNNCC 2 cut(s) 7, 438
NmuCI GTSAC 1 cut(s) 302
NspI RCATGY 3 cut(s) 95, 374, 421
PaeI GCATGC 2 cut(s) 374, 421
PciI ACATGT 1 cut(s) 91
PdmI GAANNNNTTC 1 cut(s) 144
PfeI GAWTC 1 cut(s) 112
PkrI GCNGC 2 cut(s) 214, 466
Ple19I CGATCG 1 cut(s) 165
PleI GAGTC 3 cut(s) 223, 299, 320
PpsI GAGTC 3 cut(s) 223, 299, 320
PscI ACATGT 1 cut(s) 91
Psp6I CCWGG 1 cut(s) 234
PspGI CCWGG 1 cut(s) 234
PspN4I GGNNCC 2 cut(s) 7, 438
PspPI GGNCC 1 cut(s) 214
PstI CTGCAG 1 cut(s) 87
PsuI RGATCY 2 cut(s) 436, 483
PvuI CGATCG 1 cut(s) 165
RsaI GTAC 1 cut(s) 63
RsaNI GTAC 1 cut(s) 62
RseI CAYNNNNRTG 2 cut(s) 300, 387
SaqAI TTAA 1 cut(s) 273
SatI GCNGC 2 cut(s) 213, 465
Sau3AI GATC 3 cut(s) 162, 436, 483
Sau96I GGNCC 1 cut(s) 214
SchI GAGTC 3 cut(s) 223, 299, 321
ScrFI CCNGG 1 cut(s) 236
SetI ASST 6 cut(s) 81, 154, 335, 401, 544, 560
SfaNI GCATC 1 cut(s) 391
SfcI CTRYAG 1 cut(s) 83
SmiMI CAYNNNNRTG 2 cut(s) 300, 387
SphI GCATGC 2 cut(s) 374, 421
Sse9I AATT 2 cut(s) 56, 347
SsiI CCGC 2 cut(s) 212, 591
StyD4I CCNGG 1 cut(s) 234
StyI CCWWGG 2 cut(s) 134, 441
TaaI ACNGT 3 cut(s) 29, 190, 535
TaiI ACGT 2 cut(s) 81, 401
TaqI TCGA 2 cut(s) 223, 344
TasI AATT 2 cut(s) 56, 347
TauI GCSGC 1 cut(s) 215
TfiI GAWTC 1 cut(s) 112
Tru1I TTAA 1 cut(s) 273
Tru9I TTAA 1 cut(s) 273
TscAI CASTG 2 cut(s) 199, 307
TseFI GTSAC 1 cut(s) 302
TseI GCWGC 1 cut(s) 464
Tsp45I GTSAC 1 cut(s) 302
TspDTI ATGAA 1 cut(s) 420
TspGWI ACGGA 1 cut(s) 330
TspRI CASTG 2 cut(s) 199, 307
XapI RAATTY 2 cut(s) 56, 347
XceI RCATGY 3 cut(s) 95, 374, 421
XmnI GAANNNNTTC 1 cut(s) 144
ZraI GACGTC 1 cut(s) 399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.