FvH4_4g34380

AMSH-like ubiquitin thioesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0002227
Physical Location & Seq
Reverse (-)
32201212 .. 32204814
3603 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g34380.t2

Sequence Viewer

Length: 813 bp
ATGGCGGAAAGAAACCTTTTAAGCTTGAGAATCATCAATGGGGAACAAGCTACTTTGTCATCGTTGGATTCAGTGGAATCTGGCAGTAGTTGCTTGGCTTGTAAATTTTTTGCTCCAAGCGAATTGCAGAATATTACAGTTCATGCTGTTACGCAATGCACCCCTTCTCCTTTAATCTCCTGCACAGCAAGTATGCCTCAAGGTGCTACTGTCTCACAAATTACAGCCGCCAATTCAGAACAGCTGTGTGATCAATCATCATCATCATCTAGAGTACTGCGAGATGTACATATCTCAGCTCGGTTAATGGAAGATTTCCTGCAGCTTGCTAAAGAAAATACAGACAAGGATCTTGAAACATGTGGAACTCTTGGCGCCTTTCTTAAAAATGAAACATTCTACGTGACGACGCTGATCATACCCAAGCAGGAATCCACTTCCAGCTCTTGTCAGGCAATAAATGACGAGGAAGTCTTTGCAATACAAAATGAACAATCACTTTTTCCAGCAGGATGGATCCATACGCATCCTTCTCAGAGTTGTTTCATGTCATCAGTGGATTTACACACACATTATTCATTTCAGGTTATGGTACCTGAGGCTTTTGCCATTGTCATGGCCCCAACTGATACTTCAAGAAGCTATGGAATATTCCGGCTATCTGAGCCTGGTGGGATGAATGTCCTGAAAGAGTGCCAAGAGGAAGGGTTCCACACTCACAAAGAAACAACAGATGGGAGTCCTATCTACGAGCACTGCTCCAATGTCTACACAAACTCAAATCTGAGGTTTGAAATCTTCGACTTACGTTGA

Protein Analysis

271

Amino Acids

29.77

Weight (kDa)

4.84

Isoelectric Point (pI)

70.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
JAB PF01398 93 - 200 2.6e-16 JAB1/Mov34/MPN/PAD-1 ubiquitin protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 470
Acc65I GGTACC 1 cut(s) 592
AccB1I GGYRCC 2 cut(s) 374, 592
AccI GTMKAC 1 cut(s) 768
AciI CCGC 2 cut(s) 5, 228
AclWI GGATC 3 cut(s) 357, 511, 524
AcsI RAATTY 1 cut(s) 104
AcyI GRCGYC 1 cut(s) 375
AfaI GTAC 3 cut(s) 276, 288, 594
AflIII ACRYGT 1 cut(s) 359
AgsI TTSAA 3 cut(s) 356, 636, 794
AjnI CCWGG 1 cut(s) 667
AjuI GAANNNNNNNTTGG 2 cut(s) 616, 648
AluBI AGCT 7 cut(s) 24, 50, 244, 299, 325, 444, 642
AluI AGCT 7 cut(s) 24, 50, 244, 299, 325, 444, 642
Alw21I GWGCWC 1 cut(s) 756
Alw26I GTCTC 1 cut(s) 217
AlwI GGATC 3 cut(s) 357, 511, 524
AoxI GGCC 1 cut(s) 618
ApeKI GCWGC 1 cut(s) 322
ApoI RAATTY 1 cut(s) 104
Asp718I GGTACC 1 cut(s) 592
AspLEI GCGC 1 cut(s) 377
AspS9I GGNCC 1 cut(s) 619
AxyI CCTNAGG 1 cut(s) 597
BamHI GGATCC 1 cut(s) 516
BanI GGYRCC 2 cut(s) 374, 592
Bbv12I GWGCWC 1 cut(s) 756
BbvI GCAGC 1 cut(s) 334
BccI CCATC 2 cut(s) 507, 728
BciT130I CCWGG 1 cut(s) 669
BclI TGATCA 2 cut(s) 250, 414
BcoDI GTCTC 1 cut(s) 217
BfaI CTAG 1 cut(s) 270
BfmI CTRYAG 1 cut(s) 320
BfoI RGCGCY 1 cut(s) 378
BisI GCNGC 2 cut(s) 228, 323
BlsI GCNGC 2 cut(s) 229, 324
BmcAI AGTACT 1 cut(s) 276
Bme1390I CCNGG 1 cut(s) 669
BmgT120I GGNCC 1 cut(s) 619
BmiI GGNNCC 5 cut(s) 376, 518, 594, 621, 710
BmrFI CCNGG 1 cut(s) 669
BmsI GCATC 1 cut(s) 535
BplI GAGNNNNNCTC 2 cut(s) 743, 775
BpuEI CTTGAG 2 cut(s) 46, 183
BsaAI YACGTR 1 cut(s) 403
BsaHI GRCGYC 1 cut(s) 375
BsaXI ACNNNNNCTCC 2 cut(s) 151, 181
Bse21I CCTNAGG 1 cut(s) 597
Bse3DI GCAATG 1 cut(s) 161
BseBI CCWGG 1 cut(s) 669
BseGI GGATG 3 cut(s) 518, 526, 681
BseMI GCAATG 1 cut(s) 161
BseMII CTCAG 5 cut(s) 309, 548, 588, 654, 776
BseXI GCAGC 1 cut(s) 334
BsgI GTGCAG 1 cut(s) 166
BshFI GGCC 1 cut(s) 620
BshNI GGYRCC 2 cut(s) 374, 592
BsiHKAI GWGCWC 1 cut(s) 756
BsiSI CCGG 1 cut(s) 655
BsmAI GTCTC 1 cut(s) 217
BsnI GGCC 1 cut(s) 620
Bsp1286I GDGCHC 1 cut(s) 756
Bsp1407I TGTACA 1 cut(s) 286
Bsp143I GATC 4 cut(s) 250, 349, 414, 516
BspACI CCGC 2 cut(s) 5, 228
BspANI GGCC 1 cut(s) 620
BspCNI CTCAG 5 cut(s) 308, 547, 589, 655, 777
BspLI GGNNCC 5 cut(s) 376, 518, 594, 621, 710
BspMAI CTGCAG 1 cut(s) 324
BspPI GGATC 3 cut(s) 357, 511, 524
BspT107I GGYRCC 2 cut(s) 374, 592
BsrDI GCAATG 1 cut(s) 161
BsrGI TGTACA 1 cut(s) 286
BssMI GATC 4 cut(s) 250, 349, 414, 516
BssNI GRCGYC 1 cut(s) 375
Bst2UI CCWGG 1 cut(s) 669
Bst4CI ACNGT 2 cut(s) 139, 211
BstACI GRCGYC 1 cut(s) 375
BstAPI GCANNNNNTGC 1 cut(s) 90
BstAUI TGTACA 1 cut(s) 286
BstBAI YACGTR 1 cut(s) 403
BstC8I GCNNGC 1 cut(s) 327
BstDEI CTNAG 5 cut(s) 295, 534, 597, 663, 785
BstF5I GGATG 3 cut(s) 518, 526, 681
BstH2I RGCGCY 1 cut(s) 378
BstHHI GCGC 1 cut(s) 377
BstKTI GATC 4 cut(s) 253, 352, 417, 519
BstMAI GTCTC 1 cut(s) 217
BstMBI GATC 4 cut(s) 250, 349, 414, 516
BstMWI GCNNNNNNNGC 2 cut(s) 90, 664
BstNI CCWGG 1 cut(s) 669
BstNSI RCATGY 1 cut(s) 363
BstSCI CCNGG 1 cut(s) 667
BstSFI CTRYAG 1 cut(s) 320
BstV1I GCAGC 1 cut(s) 334
BstX2I RGATCY 2 cut(s) 349, 516
BstXI CCANNNNNNTGG 2 cut(s) 513, 616
BstYI RGATCY 2 cut(s) 349, 516
Bsu36I CCTNAGG 1 cut(s) 597
BsuRI GGCC 1 cut(s) 620
BtsCI GGATG 3 cut(s) 518, 526, 681
BtsI GCAGTG 1 cut(s) 754
BtsIMutI CAGTG 3 cut(s) 78, 561, 754
Cac8I GCNNGC 1 cut(s) 327
CfoI GCGC 1 cut(s) 377
Cfr13I GGNCC 1 cut(s) 619
CseI GACGC 1 cut(s) 418
Csp6I GTAC 3 cut(s) 275, 287, 593
CviAII CATG 4 cut(s) 143, 360, 547, 616
CviQI GTAC 3 cut(s) 275, 287, 593
DdeI CTNAG 5 cut(s) 295, 534, 597, 663, 785
DinI GGCGCC 1 cut(s) 376
DpnI GATC 4 cut(s) 252, 351, 416, 518
DpnII GATC 4 cut(s) 250, 349, 414, 516
DrdI GACNNNNNNGTC 1 cut(s) 470
DseDI GACNNNNNNGTC 1 cut(s) 470
EciI GGCGGA 1 cut(s) 20
Eco81I CCTNAGG 1 cut(s) 597
EcoRII CCWGG 1 cut(s) 667
EgeI GGCGCC 1 cut(s) 376
EheI GGCGCC 1 cut(s) 376
FaeI CATG 4 cut(s) 146, 363, 550, 619
FatI CATG 4 cut(s) 142, 359, 546, 615
FbaI TGATCA 2 cut(s) 250, 414
FblI GTMKAC 1 cut(s) 768
Fnu4HI GCNGC 2 cut(s) 228, 323
FokI GGATG 3 cut(s) 513, 525, 688
Fsp4HI GCNGC 2 cut(s) 228, 323
FspBI CTAG 1 cut(s) 270
GlaI GCGC 1 cut(s) 376
GluI GCNGC 2 cut(s) 228, 323
HaeII RGCGCY 1 cut(s) 378
HaeIII GGCC 1 cut(s) 620
HapII CCGG 1 cut(s) 655
HgaI GACGC 1 cut(s) 418
HhaI GCGC 1 cut(s) 377
Hin1I GRCGYC 1 cut(s) 375
Hin1II CATG 4 cut(s) 146, 363, 550, 619
Hin6I GCGC 1 cut(s) 375
HinP1I GCGC 1 cut(s) 375
HindIII AAGCTT 1 cut(s) 22
HinfI GANTC 5 cut(s) 30, 68, 77, 431, 739
HpaII CCGG 1 cut(s) 655
Hpy166II GTNNAC 1 cut(s) 769
Hpy188I TCNGA 4 cut(s) 238, 537, 664, 786
Hpy188III TCNNGA 4 cut(s) 270, 353, 636, 685
Hpy8I GTNNAC 1 cut(s) 769
Hpy99I CGWCG 1 cut(s) 412
HpyAV CCTTC 3 cut(s) 174, 540, 698
HpyCH4III ACNGT 2 cut(s) 139, 211
HpyCH4IV ACGT 2 cut(s) 402, 808
HpyCH4V TGCA 5 cut(s) 127, 159, 183, 322, 479
HpyF10VI GCNNNNNNNGC 2 cut(s) 90, 664
HpyF3I CTNAG 5 cut(s) 295, 534, 597, 663, 785
HpySE526I ACGT 2 cut(s) 402, 808
Hsp92I GRCGYC 1 cut(s) 375
Hsp92II CATG 4 cut(s) 146, 363, 550, 619
HspAI GCGC 1 cut(s) 375
KasI GGCGCC 1 cut(s) 374
KpnI GGTACC 1 cut(s) 596
Ksp22I TGATCA 2 cut(s) 250, 414
Kzo9I GATC 4 cut(s) 250, 349, 414, 516
LmnI GCTCC 2 cut(s) 118, 764
Lsp1109I GCAGC 1 cut(s) 334
LweI GCATC 1 cut(s) 535
MaeI CTAG 1 cut(s) 270
MaeII ACGT 2 cut(s) 402, 808
MaeIII GTNAC 2 cut(s) 148, 403
MalI GATC 4 cut(s) 252, 351, 416, 518
MboI GATC 4 cut(s) 250, 349, 414, 516
MboII GAAGA 2 cut(s) 323, 790
MflI RGATCY 2 cut(s) 349, 516
MhlI GDGCHC 1 cut(s) 756
MluCI AATT 4 cut(s) 104, 122, 219, 232
Mly113I GGCGCC 1 cut(s) 375
MlyI GAGTC 1 cut(s) 748
MmeI TCCRAC 1 cut(s) 45
MnlI CCTC 5 cut(s) 207, 460, 592, 694, 780
MseI TTAA 4 cut(s) 20, 173, 305, 384
MslI CAYNNNNRTG 1 cut(s) 614
MspA1I CMGCKG 1 cut(s) 244
MspI CCGG 1 cut(s) 655
MspR9I CCNGG 1 cut(s) 669
MvaI CCWGG 1 cut(s) 669
MwoI GCNNNNNNNGC 2 cut(s) 90, 664
NarI GGCGCC 1 cut(s) 375
NdeII GATC 4 cut(s) 250, 349, 414, 516
NlaIII CATG 4 cut(s) 146, 363, 550, 619
NlaIV GGNNCC 5 cut(s) 376, 518, 594, 621, 710
NmuCI GTSAC 1 cut(s) 403
NspI RCATGY 1 cut(s) 363
PciI ACATGT 1 cut(s) 359
PfeI GAWTC 4 cut(s) 30, 68, 77, 431
PkrI GCNGC 2 cut(s) 229, 324
PleI GAGTC 1 cut(s) 747
PluTI GGCGCC 1 cut(s) 378
PpsI GAGTC 1 cut(s) 747
Ppu21I YACGTR 1 cut(s) 403
PscI ACATGT 1 cut(s) 359
Psp6I CCWGG 1 cut(s) 667
PspGI CCWGG 1 cut(s) 667
PspN4I GGNNCC 5 cut(s) 376, 518, 594, 621, 710
PspPI GGNCC 1 cut(s) 619
PstI CTGCAG 1 cut(s) 324
PsuI RGATCY 2 cut(s) 349, 516
PvuII CAGCTG 1 cut(s) 244
RsaI GTAC 3 cut(s) 276, 288, 594
RsaNI GTAC 3 cut(s) 275, 287, 593
RseI CAYNNNNRTG 1 cut(s) 614
SaqAI TTAA 4 cut(s) 20, 173, 305, 384
SatI GCNGC 2 cut(s) 228, 323
Sau3AI GATC 4 cut(s) 250, 349, 414, 516
Sau96I GGNCC 1 cut(s) 619
ScaI AGTACT 1 cut(s) 276
SchI GAGTC 1 cut(s) 748
ScrFI CCNGG 1 cut(s) 669
SduI GDGCHC 1 cut(s) 756
SfaNI GCATC 1 cut(s) 535
SfcI CTRYAG 1 cut(s) 320
SfoI GGCGCC 1 cut(s) 376
SmiMI CAYNNNNRTG 1 cut(s) 614
SmlI CTYRAG 2 cut(s) 25, 198
SmoI CTYRAG 2 cut(s) 25, 198
Sse9I AATT 4 cut(s) 104, 122, 219, 232
SsiI CCGC 2 cut(s) 5, 228
SspDI GGCGCC 1 cut(s) 374
SspI AATATT 2 cut(s) 133, 651
SspMI CTAG 1 cut(s) 270
StyD4I CCNGG 1 cut(s) 667
TaaI ACNGT 2 cut(s) 139, 211
TaiI ACGT 2 cut(s) 405, 811
TaqI TCGA 1 cut(s) 801
TasI AATT 4 cut(s) 104, 122, 219, 232
TatI WGTACW 2 cut(s) 274, 286
TauI GCSGC 1 cut(s) 230
TfiI GAWTC 4 cut(s) 30, 68, 77, 431
Tru1I TTAA 4 cut(s) 20, 173, 305, 384
Tru9I TTAA 4 cut(s) 20, 173, 305, 384
TscAI CASTG 3 cut(s) 78, 561, 761
TseFI GTSAC 1 cut(s) 403
TseI GCWGC 1 cut(s) 322
Tsp45I GTSAC 1 cut(s) 403
TspDTI ATGAA 6 cut(s) 131, 405, 504, 535, 567, 692
TspRI CASTG 3 cut(s) 78, 561, 761
XapI RAATTY 1 cut(s) 104
XbaI TCTAGA 1 cut(s) 269
XceI RCATGY 1 cut(s) 363
XmiI GTMKAC 1 cut(s) 768
XspI CTAG 1 cut(s) 270
ZrmI AGTACT 1 cut(s) 276
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.