RLG00000005888

AMSH-like ubiquitin thioesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0002227
Physical Location & Seq
Forward (+)
2583956 .. 2586693
2738 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005888

Sequence Viewer

Length: 858 bp
ATGTGCGATATTGACTGGAAGGTCCAAAGTAATGTTAGAAGTTGTGCTATGGCAGAAAGAAACCATCTAAGCTTGAGAATGATCAATGGGGAGCAAGCTAATTTGTCATCTTTGAATTCGTTGGAAGCTGGCAGTAGTTGCTTGGCTTGCAAATTTCTTGCTCCAAGCGAATGGCAGAATATTACAGTTCATACTGTTACGCAATTCTCCCCTTCTCCTTTAATCTCTTGCACAGAAAGTATGCCTCAAGGTGCTACTGTTTCACAAATTACAGCCGCGAATTCAGAGCAGTCATGTGATCAATCAACATCATCTAGGGTCCTGCGAGATGTACATATCTCAGCTCGGTTAATGGAAGATTTCCTTCAGCTTGCTGAGGACAACACAGAGAAGGATCTTGAAACCTGTGGGACTCTTGGCGCCTTTCTTAAAAATGAAACATTCTACGTGACTAGTCTGATCATACCCAAGCAGGAATCCACTTCCAACTCTTGTCAGGCAACAAATGACGAGGAAGTCTTTGCCATACAAAATGAACAATCACTTTTTCCTGTAGGATGGATCCATACACATCCTTCTCAGAGTTGTTTTATGTCATCAGTGGATTTACACACACATTATTCACGTCAGGTTATGGTACCTGAGGCTTTTGCCATTGTCATGGCCCCAACTGATACTTCAAGAAGCTATGGAATATTCCGGCTATCTGACCCTGGTGGGATGAGTGTCTTGAAAGAGTGCCAAGAGACAGGGTTCCACACTCACGAAGAAACAGGAGATGGGAGTCCAATTTACGAGCACTGCTCCAACGTGTACACAAACTCAAATCTGAGGTTTGAAATCTTTGACTTGCGTTGA

Protein Analysis

286

Amino Acids

31.64

Weight (kDa)

4.81

Isoelectric Point (pI)

70.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
JAB PF01398 108 - 215 9.6e-17 JAB1/Mov34/MPN/PAD-1 ubiquitin protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 20, 515
Acc65I GGTACC 1 cut(s) 637
AccB1I GGYRCC 2 cut(s) 419, 637
AccII CGCG 1 cut(s) 278
AciI CCGC 1 cut(s) 276
AclWI GGATC 3 cut(s) 402, 556, 569
AcsI RAATTY 3 cut(s) 115, 152, 280
AcuI CTGAAG 1 cut(s) 350
AcyI GRCGYC 1 cut(s) 420
AfaI GTAC 3 cut(s) 333, 639, 815
AflIII ACRYGT 1 cut(s) 810
AgsI TTSAA 5 cut(s) 115, 401, 681, 733, 839
AhlI ACTAGT 1 cut(s) 452
AjiI CACGTC 1 cut(s) 626
AjnI CCWGG 1 cut(s) 712
AjuI GAANNNNNNNTTGG 2 cut(s) 661, 693
AluBI AGCT 6 cut(s) 72, 98, 128, 344, 370, 687
AluI AGCT 6 cut(s) 72, 98, 128, 344, 370, 687
Alw21I GWGCWC 1 cut(s) 801
Alw26I GTCTC 1 cut(s) 740
AlwI GGATC 3 cut(s) 402, 556, 569
AoxI GGCC 1 cut(s) 663
ApoI RAATTY 3 cut(s) 115, 152, 280
Asp718I GGTACC 1 cut(s) 637
AspLEI GCGC 1 cut(s) 422
AspS9I GGNCC 3 cut(s) 22, 319, 664
AvaII GGWCC 2 cut(s) 22, 319
AxyI CCTNAGG 1 cut(s) 642
BamHI GGATCC 1 cut(s) 561
BanI GGYRCC 2 cut(s) 419, 637
Bbv12I GWGCWC 1 cut(s) 801
BbvCI CCTCAGC 1 cut(s) 375
BccI CCATC 3 cut(s) 72, 552, 773
BciT130I CCWGG 1 cut(s) 714
BclI TGATCA 3 cut(s) 81, 298, 459
BcoDI GTCTC 1 cut(s) 740
BcuI ACTAGT 1 cut(s) 452
BfaI CTAG 2 cut(s) 315, 453
BfmI CTRYAG 1 cut(s) 552
BfoI RGCGCY 1 cut(s) 423
BisI GCNGC 1 cut(s) 276
BlsI GCNGC 1 cut(s) 277
Bme1390I CCNGG 1 cut(s) 714
Bme18I GGWCC 2 cut(s) 22, 319
BmgBI CACGTC 1 cut(s) 626
BmgT120I GGNCC 3 cut(s) 22, 319, 664
BmiI GGNNCC 6 cut(s) 320, 421, 563, 639, 666, 755
BmrFI CCNGG 1 cut(s) 714
BplI GAGNNNNNCTC 2 cut(s) 788, 820
Bpu10I CCTNAGC 1 cut(s) 375
BpuEI CTTGAG 2 cut(s) 94, 231
BsaAI YACGTR 1 cut(s) 448
BsaHI GRCGYC 1 cut(s) 420
BsaJI CCNNGG 1 cut(s) 712
Bse1I ACTGG 1 cut(s) 20
Bse21I CCTNAGG 1 cut(s) 642
BseBI CCWGG 1 cut(s) 714
BseDI CCNNGG 1 cut(s) 712
BseGI GGATG 3 cut(s) 563, 571, 726
BseMII CTCAG 5 cut(s) 354, 366, 593, 633, 821
BseNI ACTGG 1 cut(s) 20
Bsh1236I CGCG 1 cut(s) 278
BshFI GGCC 1 cut(s) 665
BshNI GGYRCC 2 cut(s) 419, 637
BsiHKAI GWGCWC 1 cut(s) 801
BsiSI CCGG 1 cut(s) 700
BslFI GGGAC 1 cut(s) 424
BsmAI GTCTC 1 cut(s) 740
BsmFI GGGAC 1 cut(s) 424
BsnI GGCC 1 cut(s) 665
Bsp1286I GDGCHC 1 cut(s) 801
Bsp1407I TGTACA 2 cut(s) 331, 813
Bsp143I GATC 5 cut(s) 81, 298, 394, 459, 561
BspACI CCGC 1 cut(s) 276
BspANI GGCC 1 cut(s) 665
BspCNI CTCAG 5 cut(s) 353, 367, 592, 634, 822
BspFNI CGCG 1 cut(s) 278
BspLI GGNNCC 6 cut(s) 320, 421, 563, 639, 666, 755
BspPI GGATC 3 cut(s) 402, 556, 569
BspT107I GGYRCC 2 cut(s) 419, 637
BsrGI TGTACA 2 cut(s) 331, 813
BsrI ACTGG 1 cut(s) 20
BssECI CCNNGG 1 cut(s) 712
BssMI GATC 5 cut(s) 81, 298, 394, 459, 561
BssNI GRCGYC 1 cut(s) 420
Bst2UI CCWGG 1 cut(s) 714
Bst4CI ACNGT 3 cut(s) 187, 196, 259
BstACI GRCGYC 1 cut(s) 420
BstAPI GCANNNNNTGC 1 cut(s) 138
BstAUI TGTACA 2 cut(s) 331, 813
BstBAI YACGTR 1 cut(s) 448
BstC8I GCNNGC 4 cut(s) 96, 130, 148, 372
BstDEI CTNAG 6 cut(s) 68, 340, 375, 579, 642, 830
BstF5I GGATG 3 cut(s) 563, 571, 726
BstFNI CGCG 1 cut(s) 278
BstH2I RGCGCY 1 cut(s) 423
BstHHI GCGC 1 cut(s) 422
BstKTI GATC 5 cut(s) 84, 301, 397, 462, 564
BstMAI GTCTC 1 cut(s) 740
BstMBI GATC 5 cut(s) 81, 298, 394, 459, 561
BstMWI GCNNNNNNNGC 2 cut(s) 138, 147
BstNI CCWGG 1 cut(s) 714
BstSCI CCNGG 1 cut(s) 712
BstSFI CTRYAG 1 cut(s) 552
BstUI CGCG 1 cut(s) 278
BstX2I RGATCY 2 cut(s) 394, 561
BstXI CCANNNNNNTGG 2 cut(s) 171, 661
BstYI RGATCY 2 cut(s) 394, 561
Bsu36I CCTNAGG 1 cut(s) 642
BsuRI GGCC 1 cut(s) 665
BtrI CACGTC 1 cut(s) 626
BtsCI GGATG 3 cut(s) 563, 571, 726
BtsI GCAGTG 1 cut(s) 799
BtsIMutI CAGTG 2 cut(s) 606, 799
Cac8I GCNNGC 4 cut(s) 96, 130, 148, 372
CfoI GCGC 1 cut(s) 422
Cfr13I GGNCC 3 cut(s) 22, 319, 664
Csp6I GTAC 3 cut(s) 332, 638, 814
CviAII CATG 2 cut(s) 294, 661
CviQI GTAC 3 cut(s) 332, 638, 814
DdeI CTNAG 6 cut(s) 68, 340, 375, 579, 642, 830
DinI GGCGCC 1 cut(s) 421
DpnI GATC 5 cut(s) 83, 300, 396, 461, 563
DpnII GATC 5 cut(s) 81, 298, 394, 459, 561
DrdI GACNNNNNNGTC 2 cut(s) 20, 515
DseDI GACNNNNNNGTC 2 cut(s) 20, 515
Eco47I GGWCC 2 cut(s) 22, 319
Eco57I CTGAAG 1 cut(s) 350
Eco81I CCTNAGG 1 cut(s) 642
EcoO109I RGGNCCY 1 cut(s) 319
EcoRI GAATTC 2 cut(s) 115, 280
EcoRII CCWGG 1 cut(s) 712
EgeI GGCGCC 1 cut(s) 421
EheI GGCGCC 1 cut(s) 421
FaeI CATG 2 cut(s) 297, 664
FalI AAGNNNNNCTT 2 cut(s) 348, 380
FaqI GGGAC 1 cut(s) 424
FatI CATG 2 cut(s) 293, 660
FbaI TGATCA 3 cut(s) 81, 298, 459
Fnu4HI GCNGC 1 cut(s) 276
FokI GGATG 3 cut(s) 558, 570, 733
Fsp4HI GCNGC 1 cut(s) 276
FspBI CTAG 2 cut(s) 315, 453
GlaI GCGC 1 cut(s) 421
GluI GCNGC 1 cut(s) 276
HaeII RGCGCY 1 cut(s) 423
HaeIII GGCC 1 cut(s) 665
HapII CCGG 1 cut(s) 700
HhaI GCGC 1 cut(s) 422
Hin1I GRCGYC 1 cut(s) 420
Hin1II CATG 2 cut(s) 297, 664
Hin6I GCGC 1 cut(s) 420
HinP1I GCGC 1 cut(s) 420
HindIII AAGCTT 1 cut(s) 70
HinfI GANTC 3 cut(s) 412, 476, 784
HpaII CCGG 1 cut(s) 700
Hpy166II GTNNAC 2 cut(s) 814, 816
Hpy188I TCNGA 5 cut(s) 286, 459, 582, 709, 831
Hpy188III TCNNGA 4 cut(s) 398, 681, 730, 764
Hpy8I GTNNAC 2 cut(s) 814, 816
HpyAV CCTTC 5 cut(s) 13, 222, 374, 385, 585
HpyCH4III ACNGT 3 cut(s) 187, 196, 259
HpyCH4IV ACGT 3 cut(s) 447, 625, 810
HpyCH4V TGCA 2 cut(s) 150, 231
HpyF10VI GCNNNNNNNGC 2 cut(s) 138, 147
HpyF3I CTNAG 6 cut(s) 68, 340, 375, 579, 642, 830
HpySE526I ACGT 3 cut(s) 447, 625, 810
Hsp92I GRCGYC 1 cut(s) 420
Hsp92II CATG 2 cut(s) 297, 664
HspAI GCGC 1 cut(s) 420
KasI GGCGCC 1 cut(s) 419
KpnI GGTACC 1 cut(s) 641
Ksp22I TGATCA 3 cut(s) 81, 298, 459
Kzo9I GATC 5 cut(s) 81, 298, 394, 459, 561
LmnI GCTCC 3 cut(s) 91, 166, 809
MaeI CTAG 2 cut(s) 315, 453
MaeII ACGT 3 cut(s) 447, 625, 810
MaeIII GTNAC 2 cut(s) 196, 448
MalI GATC 5 cut(s) 83, 300, 396, 461, 563
MboI GATC 5 cut(s) 81, 298, 394, 459, 561
MboII GAAGA 2 cut(s) 368, 779
MflI RGATCY 2 cut(s) 394, 561
MhlI GDGCHC 1 cut(s) 801
MluCI AATT 7 cut(s) 100, 115, 152, 203, 267, 280, 789
Mly113I GGCGCC 1 cut(s) 420
MlyI GAGTC 2 cut(s) 406, 793
MmeI TCCRAC 3 cut(s) 102, 510, 831
MnlI CCTC 5 cut(s) 255, 370, 505, 637, 825
MseI TTAA 3 cut(s) 221, 350, 429
MslI CAYNNNNRTG 1 cut(s) 659
MspI CCGG 1 cut(s) 700
MspR9I CCNGG 1 cut(s) 714
MvaI CCWGG 1 cut(s) 714
MvnI CGCG 1 cut(s) 278
MwoI GCNNNNNNNGC 2 cut(s) 138, 147
NarI GGCGCC 1 cut(s) 420
NdeII GATC 5 cut(s) 81, 298, 394, 459, 561
NlaIII CATG 2 cut(s) 297, 664
NlaIV GGNNCC 6 cut(s) 320, 421, 563, 639, 666, 755
NmuCI GTSAC 1 cut(s) 448
PfeI GAWTC 1 cut(s) 476
PkrI GCNGC 1 cut(s) 277
PleI GAGTC 2 cut(s) 406, 792
PluTI GGCGCC 1 cut(s) 423
PpsI GAGTC 2 cut(s) 406, 792
Ppu21I YACGTR 1 cut(s) 448
PpuMI RGGWCCY 1 cut(s) 319
Psp5II RGGWCCY 1 cut(s) 319
Psp6I CCWGG 1 cut(s) 712
PspGI CCWGG 1 cut(s) 712
PspN4I GGNNCC 6 cut(s) 320, 421, 563, 639, 666, 755
PspPI GGNCC 3 cut(s) 22, 319, 664
PspPPI RGGWCCY 1 cut(s) 319
PsuI RGATCY 2 cut(s) 394, 561
RsaI GTAC 3 cut(s) 333, 639, 815
RsaNI GTAC 3 cut(s) 332, 638, 814
RseI CAYNNNNRTG 1 cut(s) 659
SaqAI TTAA 3 cut(s) 221, 350, 429
SatI GCNGC 1 cut(s) 276
Sau3AI GATC 5 cut(s) 81, 298, 394, 459, 561
Sau96I GGNCC 3 cut(s) 22, 319, 664
SchI GAGTC 2 cut(s) 406, 793
ScrFI CCNGG 1 cut(s) 714
SduI GDGCHC 1 cut(s) 801
SfcI CTRYAG 1 cut(s) 552
SfoI GGCGCC 1 cut(s) 421
SinI GGWCC 2 cut(s) 22, 319
SmiMI CAYNNNNRTG 1 cut(s) 659
SmlI CTYRAG 2 cut(s) 73, 246
SmoI CTYRAG 2 cut(s) 73, 246
SpeI ACTAGT 1 cut(s) 452
Sse9I AATT 7 cut(s) 100, 115, 152, 203, 267, 280, 789
SsiI CCGC 1 cut(s) 276
SspDI GGCGCC 1 cut(s) 419
SspI AATATT 2 cut(s) 181, 696
SspMI CTAG 2 cut(s) 315, 453
StyD4I CCNGG 1 cut(s) 712
TaaI ACNGT 3 cut(s) 187, 196, 259
TaiI ACGT 3 cut(s) 450, 628, 813
TasI AATT 7 cut(s) 100, 115, 152, 203, 267, 280, 789
TatI WGTACW 2 cut(s) 331, 813
TauI GCSGC 1 cut(s) 278
TfiI GAWTC 1 cut(s) 476
Tru1I TTAA 3 cut(s) 221, 350, 429
Tru9I TTAA 3 cut(s) 221, 350, 429
TscAI CASTG 2 cut(s) 606, 806
TseFI GTSAC 1 cut(s) 448
Tsp45I GTSAC 1 cut(s) 448
TspDTI ATGAA 3 cut(s) 179, 450, 549
TspRI CASTG 2 cut(s) 606, 806
VpaK11BI GGWCC 2 cut(s) 22, 319
XapI RAATTY 3 cut(s) 115, 152, 280
XspI CTAG 2 cut(s) 315, 453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.