Rroxscaffold_5G00384370

AMSH-like ubiquitin thioesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0002227
Physical Location & Seq
Reverse (-)
63774413 .. 63777291
2879 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00384370.1

Sequence Viewer

Length: 903 bp
ATGTGTGAAGCACTTTGTGTTTGGTTGTTAGTTTTGTTTAAGCACCTTGGGAATTATTGTAATGAGGTTAGAAGTTGTGCTATGGCGAAAAGAGACCATCTAAGCTTGAGAATGATCAATGGGGAACAAGCTACTTTGTCATCTTTGAATTCGTTGGAATCTGGCAGTAGTTGCTTGGCTTGCAAATTTCTTGCTCCAAGCGAATGGCAGAATATTACAGTTCATACTGTTACGCAATTCTCCCCTTCTCCTTTAATCTCTTGCACAGAAAGTATGCCTCAAGGTGCCACTGTTTCACAAATTACAGCCGCGAATTCAGAACAGTCATGTGATCAATCAACATCATCTAGGGTCCTGCGAGATGTACATATCTCAGCTCGGTTAATGGAAGATTTCCTTCAGCTTGCTAAGGACAACACAGAGAAGGATCTTGAAACCTGTGGGACTCTTGGTGCCTTTCTCAAAAATGAAACATTCTACGTGACGACTCTGATCGTACCCAAGCAGGAATCCACTTCCAACTCTTGTCAAGCAACAAATGACGAGGAAGTCTTTGCCATACAAAATGAACAATCACTTTTTCCTGTAGGATGGATCCATACACATCCTTCTCAGAGTTGTTTTATGTCATCAGTGGATTTACACACACATTATTCACGTCAGGTTATGGTACCTGAGGCTTTTGCCATTGTCATGGCCCCAACTGATACTTCAAGAAGCTATGGAATATTCCGGCTATCTGACCCTGGTGGGATGAGTGTCTTGAAAGAGTGCCAAGAGACAGGGTTCCACACTCACGAAGAAACAGGAGATGGGAGTCCCATTTACGAGCACTGCTCCAACGTCTACACAAACTCAAATCTGAGGTTTGAAATCTTTGACTTGCGTGGAGATTCTGTTTAG

Protein Analysis

300

Amino Acids

33.27

Weight (kDa)

5.01

Isoelectric Point (pI)

66.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
JAB PF01398 119 - 226 3.5e-16 JAB1/Mov34/MPN/PAD-1 ubiquitin protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 548
Acc65I GGTACC 1 cut(s) 670
AccB1I GGYRCC 3 cut(s) 284, 452, 670
AccI GTMKAC 1 cut(s) 846
AccII CGCG 1 cut(s) 311
AciI CCGC 1 cut(s) 309
AclWI GGATC 3 cut(s) 435, 589, 602
AcsI RAATTY 3 cut(s) 148, 185, 313
AcuI CTGAAG 1 cut(s) 383
AdeI CACNNNGTG 1 cut(s) 17
AfaI GTAC 3 cut(s) 366, 498, 672
AgsI TTSAA 5 cut(s) 148, 434, 714, 766, 872
AjiI CACGTC 1 cut(s) 659
AjnI CCWGG 1 cut(s) 745
AjuI GAANNNNNNNTTGG 2 cut(s) 694, 726
AluBI AGCT 5 cut(s) 105, 131, 377, 403, 720
AluI AGCT 5 cut(s) 105, 131, 377, 403, 720
Alw21I GWGCWC 1 cut(s) 834
Alw26I GTCTC 2 cut(s) 87, 773
AlwI GGATC 3 cut(s) 435, 589, 602
AoxI GGCC 1 cut(s) 696
ApoI RAATTY 3 cut(s) 148, 185, 313
Asp718I GGTACC 1 cut(s) 670
AspS9I GGNCC 2 cut(s) 352, 697
AvaII GGWCC 1 cut(s) 352
AxyI CCTNAGG 1 cut(s) 675
BamHI GGATCC 1 cut(s) 594
BanI GGYRCC 3 cut(s) 284, 452, 670
Bbv12I GWGCWC 1 cut(s) 834
BccI CCATC 3 cut(s) 105, 585, 806
BciT130I CCWGG 1 cut(s) 747
BclI TGATCA 2 cut(s) 114, 331
BcoDI GTCTC 2 cut(s) 87, 773
BfaI CTAG 1 cut(s) 348
BfmI CTRYAG 1 cut(s) 585
BisI GCNGC 1 cut(s) 309
BlsI GCNGC 1 cut(s) 310
Bme1390I CCNGG 1 cut(s) 747
Bme18I GGWCC 1 cut(s) 352
BmgBI CACGTC 1 cut(s) 659
BmgT120I GGNCC 2 cut(s) 352, 697
BmiI GGNNCC 7 cut(s) 286, 353, 454, 596, 672, 699, 788
BmrFI CCNGG 1 cut(s) 747
BplI GAGNNNNNCTC 2 cut(s) 821, 853
Bpu10I CCTNAGC 1 cut(s) 408
BpuEI CTTGAG 2 cut(s) 127, 264
BsaAI YACGTR 1 cut(s) 481
BsaI GGTCTC 1 cut(s) 87
BsaJI CCNNGG 2 cut(s) 46, 745
BsaXI ACNNNNNCTCC 1 cut(s) 882
Bse21I CCTNAGG 1 cut(s) 675
BseBI CCWGG 1 cut(s) 747
BseDI CCNNGG 2 cut(s) 46, 745
BseGI GGATG 3 cut(s) 596, 604, 759
BseMII CTCAG 4 cut(s) 387, 626, 666, 854
Bsh1236I CGCG 1 cut(s) 311
BshFI GGCC 1 cut(s) 698
BshNI GGYRCC 3 cut(s) 284, 452, 670
BsiHKAI GWGCWC 1 cut(s) 834
BsiSI CCGG 1 cut(s) 733
BslFI GGGAC 2 cut(s) 457, 804
BsmAI GTCTC 2 cut(s) 87, 773
BsmFI GGGAC 2 cut(s) 457, 804
BsnI GGCC 1 cut(s) 698
Bso31I GGTCTC 1 cut(s) 87
Bsp1286I GDGCHC 1 cut(s) 834
Bsp1407I TGTACA 1 cut(s) 364
Bsp143I GATC 5 cut(s) 114, 331, 427, 492, 594
BspACI CCGC 1 cut(s) 309
BspANI GGCC 1 cut(s) 698
BspCNI CTCAG 4 cut(s) 386, 625, 667, 855
BspFNI CGCG 1 cut(s) 311
BspLI GGNNCC 7 cut(s) 286, 353, 454, 596, 672, 699, 788
BspPI GGATC 3 cut(s) 435, 589, 602
BspT107I GGYRCC 3 cut(s) 284, 452, 670
BspTNI GGTCTC 1 cut(s) 87
BsrGI TGTACA 1 cut(s) 364
BssECI CCNNGG 2 cut(s) 46, 745
BssMI GATC 5 cut(s) 114, 331, 427, 492, 594
BssT1I CCWWGG 1 cut(s) 46
Bst2UI CCWGG 1 cut(s) 747
Bst4CI ACNGT 4 cut(s) 220, 229, 292, 324
BstAPI GCANNNNNTGC 1 cut(s) 171
BstAUI TGTACA 1 cut(s) 364
BstBAI YACGTR 1 cut(s) 481
BstC8I GCNNGC 2 cut(s) 181, 405
BstDEI CTNAG 6 cut(s) 101, 373, 408, 612, 675, 863
BstF5I GGATG 3 cut(s) 596, 604, 759
BstFNI CGCG 1 cut(s) 311
BstKTI GATC 5 cut(s) 117, 334, 430, 495, 597
BstMAI GTCTC 2 cut(s) 87, 773
BstMBI GATC 5 cut(s) 114, 331, 427, 492, 594
BstMWI GCNNNNNNNGC 2 cut(s) 171, 180
BstNI CCWGG 1 cut(s) 747
BstSCI CCNGG 1 cut(s) 745
BstSFI CTRYAG 1 cut(s) 585
BstUI CGCG 1 cut(s) 311
BstX2I RGATCY 2 cut(s) 427, 594
BstXI CCANNNNNNTGG 2 cut(s) 204, 694
BstYI RGATCY 2 cut(s) 427, 594
Bsu36I CCTNAGG 1 cut(s) 675
BsuRI GGCC 1 cut(s) 698
BtrI CACGTC 1 cut(s) 659
BtsCI GGATG 3 cut(s) 596, 604, 759
BtsI GCAGTG 1 cut(s) 832
BtsIMutI CAGTG 3 cut(s) 288, 639, 832
Cac8I GCNNGC 2 cut(s) 181, 405
Cfr13I GGNCC 2 cut(s) 352, 697
Csp6I GTAC 3 cut(s) 365, 497, 671
CviAII CATG 2 cut(s) 327, 694
CviQI GTAC 3 cut(s) 365, 497, 671
DdeI CTNAG 6 cut(s) 101, 373, 408, 612, 675, 863
DpnI GATC 5 cut(s) 116, 333, 429, 494, 596
DpnII GATC 5 cut(s) 114, 331, 427, 492, 594
DraIII CACNNNGTG 1 cut(s) 17
DrdI GACNNNNNNGTC 1 cut(s) 548
DseDI GACNNNNNNGTC 1 cut(s) 548
Eco130I CCWWGG 1 cut(s) 46
Eco31I GGTCTC 1 cut(s) 87
Eco47I GGWCC 1 cut(s) 352
Eco57I CTGAAG 1 cut(s) 383
Eco81I CCTNAGG 1 cut(s) 675
EcoO109I RGGNCCY 1 cut(s) 352
EcoRI GAATTC 2 cut(s) 148, 313
EcoRII CCWGG 1 cut(s) 745
EcoT14I CCWWGG 1 cut(s) 46
ErhI CCWWGG 1 cut(s) 46
FaeI CATG 2 cut(s) 330, 697
FalI AAGNNNNNCTT 2 cut(s) 381, 413
FaqI GGGAC 2 cut(s) 457, 804
FatI CATG 2 cut(s) 326, 693
FbaI TGATCA 2 cut(s) 114, 331
FblI GTMKAC 1 cut(s) 846
Fnu4HI GCNGC 1 cut(s) 309
FokI GGATG 3 cut(s) 591, 603, 766
Fsp4HI GCNGC 1 cut(s) 309
FspBI CTAG 1 cut(s) 348
GluI GCNGC 1 cut(s) 309
HaeIII GGCC 1 cut(s) 698
HapII CCGG 1 cut(s) 733
Hin1II CATG 2 cut(s) 330, 697
HindIII AAGCTT 1 cut(s) 103
HinfI GANTC 6 cut(s) 158, 445, 487, 509, 817, 893
HpaII CCGG 1 cut(s) 733
Hpy166II GTNNAC 1 cut(s) 847
Hpy188I TCNGA 5 cut(s) 319, 492, 615, 742, 864
Hpy188III TCNNGA 4 cut(s) 431, 714, 763, 797
Hpy8I GTNNAC 1 cut(s) 847
HpyAV CCTTC 4 cut(s) 255, 407, 418, 618
HpyCH4III ACNGT 4 cut(s) 220, 229, 292, 324
HpyCH4IV ACGT 3 cut(s) 480, 658, 843
HpyCH4V TGCA 2 cut(s) 183, 264
HpyF10VI GCNNNNNNNGC 2 cut(s) 171, 180
HpyF3I CTNAG 6 cut(s) 101, 373, 408, 612, 675, 863
HpySE526I ACGT 3 cut(s) 480, 658, 843
Hsp92II CATG 2 cut(s) 330, 697
KpnI GGTACC 1 cut(s) 674
Ksp22I TGATCA 2 cut(s) 114, 331
Kzo9I GATC 5 cut(s) 114, 331, 427, 492, 594
LmnI GCTCC 2 cut(s) 199, 842
MaeI CTAG 1 cut(s) 348
MaeII ACGT 3 cut(s) 480, 658, 843
MaeIII GTNAC 2 cut(s) 229, 481
MalI GATC 5 cut(s) 116, 333, 429, 494, 596
MboI GATC 5 cut(s) 114, 331, 427, 492, 594
MboII GAAGA 2 cut(s) 401, 812
MflI RGATCY 2 cut(s) 427, 594
MhlI GDGCHC 1 cut(s) 834
MluCI AATT 6 cut(s) 52, 148, 185, 236, 300, 313
MlyI GAGTC 3 cut(s) 439, 481, 826
MmeI TCCRAC 3 cut(s) 135, 543, 864
MnlI CCTC 5 cut(s) 58, 288, 538, 670, 858
MseI TTAA 3 cut(s) 39, 254, 383
MslI CAYNNNNRTG 1 cut(s) 692
MspI CCGG 1 cut(s) 733
MspR9I CCNGG 1 cut(s) 747
MvaI CCWGG 1 cut(s) 747
MvnI CGCG 1 cut(s) 311
MwoI GCNNNNNNNGC 2 cut(s) 171, 180
NdeII GATC 5 cut(s) 114, 331, 427, 492, 594
NlaIII CATG 2 cut(s) 330, 697
NlaIV GGNNCC 7 cut(s) 286, 353, 454, 596, 672, 699, 788
NmuCI GTSAC 1 cut(s) 481
PfeI GAWTC 3 cut(s) 158, 509, 893
PkrI GCNGC 1 cut(s) 310
PleI GAGTC 3 cut(s) 439, 481, 825
PpsI GAGTC 3 cut(s) 439, 481, 825
Ppu21I YACGTR 1 cut(s) 481
PpuMI RGGWCCY 1 cut(s) 352
Psp5II RGGWCCY 1 cut(s) 352
Psp6I CCWGG 1 cut(s) 745
PspGI CCWGG 1 cut(s) 745
PspN4I GGNNCC 7 cut(s) 286, 353, 454, 596, 672, 699, 788
PspPI GGNCC 2 cut(s) 352, 697
PspPPI RGGWCCY 1 cut(s) 352
PsuI RGATCY 2 cut(s) 427, 594
RsaI GTAC 3 cut(s) 366, 498, 672
RsaNI GTAC 3 cut(s) 365, 497, 671
RseI CAYNNNNRTG 1 cut(s) 692
SaqAI TTAA 3 cut(s) 39, 254, 383
SatI GCNGC 1 cut(s) 309
Sau3AI GATC 5 cut(s) 114, 331, 427, 492, 594
Sau96I GGNCC 2 cut(s) 352, 697
SchI GAGTC 3 cut(s) 439, 481, 826
ScrFI CCNGG 1 cut(s) 747
SduI GDGCHC 1 cut(s) 834
SfcI CTRYAG 1 cut(s) 585
SinI GGWCC 1 cut(s) 352
SmiMI CAYNNNNRTG 1 cut(s) 692
SmlI CTYRAG 2 cut(s) 106, 279
SmoI CTYRAG 2 cut(s) 106, 279
Sse9I AATT 6 cut(s) 52, 148, 185, 236, 300, 313
SsiI CCGC 1 cut(s) 309
SspI AATATT 2 cut(s) 214, 729
SspMI CTAG 1 cut(s) 348
StyD4I CCNGG 1 cut(s) 745
StyI CCWWGG 1 cut(s) 46
TaaI ACNGT 4 cut(s) 220, 229, 292, 324
TaiI ACGT 3 cut(s) 483, 661, 846
TasI AATT 6 cut(s) 52, 148, 185, 236, 300, 313
TatI WGTACW 1 cut(s) 364
TauI GCSGC 1 cut(s) 311
TfiI GAWTC 3 cut(s) 158, 509, 893
Tru1I TTAA 3 cut(s) 39, 254, 383
Tru9I TTAA 3 cut(s) 39, 254, 383
TscAI CASTG 3 cut(s) 295, 639, 839
TseFI GTSAC 1 cut(s) 481
Tsp45I GTSAC 1 cut(s) 481
TspDTI ATGAA 3 cut(s) 212, 483, 582
TspRI CASTG 3 cut(s) 295, 639, 839
VpaK11BI GGWCC 1 cut(s) 352
XapI RAATTY 3 cut(s) 148, 185, 313
XmiI GTMKAC 1 cut(s) 846
XspI CTAG 1 cut(s) 348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.