Rh4DG413800

AMSH-like ubiquitin thioesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0002227
Physical Location & Seq
Reverse (-)
62441041 .. 62444165
3125 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG413800.1

Sequence Viewer

Length: 816 bp
ATGTGTGAAGCACTTTGTGTTTGGTTGTTAGTTCTGTTTAAGCACCTTGGGAATTATTGTGACGAGGTTAGAAGTTGTGCTATGGCAGAAAGAAACCATCTAAGCTTGAGAGTCATCAATGGGGAACAAGCTACTTTGTCATCTTTGAATTCGTTGCAATCTGGCAGTAGTTGCTTGGCTCGTAAATTTCTTGCTCCAAGCGAATGGCAGAATATTACAGTTCATACTGTTACGCAATTCTCCCCTTCTCCTTTAATCTCTTGCACACAAAGTATGCCTCAAGGTGCTACTGTTTCACAAATTACAGCCGCGAATTCAGAACAGTCATGTGATCAATCAACATCATCTAGTGTCCTGCGAGATGTACATATCTCAGCTCGGTTAATGGAAGATTTCCTTCAGCTTGCTAAGGACAACACAGAGAAGGATCTTGAAACCTGTGGGACTCTTGGCGCCTTTCTTAAAAATGAAACATTCTATGTGACGACTCTGATCATACCCAAGCAGGAATCCACTTCCAACTCTTGTCAGGCAACGAATGACGAGGAAGTCTTTGCCATACAAAATGAACAATCACTTTTTCCTGTAGGATGGATCCATACACATCCTTCTCAGAGTTGTTTTATGTCATCAGTGGATTTACACACACATTATTCACGTCAGGTTATGGTACCTGAGGCTTTTGCCATTGTCATGGCCCCAACTGATACTTCAAGGTATGTAGTTCTTATGAAGCTCAGCCAAAATATTTTTACAAAAAAACCAAAAATCAAAAAACTTGGATATGACATTAAATCAGTAGGTCAGAAACGTTAG

Protein Analysis

271

Amino Acids

30.14

Weight (kDa)

6.44

Isoelectric Point (pI)

65.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
JAB PF01398 119 - 226 1e-16 JAB1/Mov34/MPN/PAD-1 ubiquitin protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 548
Acc65I GGTACC 1 cut(s) 670
AccB1I GGYRCC 2 cut(s) 452, 670
AccII CGCG 1 cut(s) 311
AciI CCGC 1 cut(s) 309
AclI AACGTT 1 cut(s) 811
AclWI GGATC 3 cut(s) 435, 589, 602
AcsI RAATTY 3 cut(s) 148, 185, 313
AcuI CTGAAG 1 cut(s) 383
AcyI GRCGYC 1 cut(s) 453
AdeI CACNNNGTG 1 cut(s) 17
AfaI GTAC 2 cut(s) 366, 672
AgsI TTSAA 3 cut(s) 148, 434, 714
AjiI CACGTC 1 cut(s) 659
AjuI GAANNNNNNNTTGG 2 cut(s) 694, 726
AluBI AGCT 5 cut(s) 105, 131, 377, 403, 736
AluI AGCT 5 cut(s) 105, 131, 377, 403, 736
AlwI GGATC 3 cut(s) 435, 589, 602
AoxI GGCC 1 cut(s) 696
ApoI RAATTY 3 cut(s) 148, 185, 313
Asp718I GGTACC 1 cut(s) 670
AspLEI GCGC 1 cut(s) 455
AspS9I GGNCC 1 cut(s) 697
AxyI CCTNAGG 1 cut(s) 675
BamHI GGATCC 1 cut(s) 594
BanI GGYRCC 2 cut(s) 452, 670
BccI CCATC 2 cut(s) 105, 585
BclI TGATCA 2 cut(s) 331, 492
BfaI CTAG 1 cut(s) 348
BfmI CTRYAG 1 cut(s) 585
BfoI RGCGCY 1 cut(s) 456
BisI GCNGC 1 cut(s) 309
BlpI GCTNAGC 1 cut(s) 737
BlsI GCNGC 1 cut(s) 310
BmgBI CACGTC 1 cut(s) 659
BmgT120I GGNCC 1 cut(s) 697
BmiI GGNNCC 4 cut(s) 454, 596, 672, 699
Bpu10I CCTNAGC 1 cut(s) 408
Bpu1102I GCTNAGC 1 cut(s) 737
BpuEI CTTGAG 2 cut(s) 127, 264
BsaHI GRCGYC 1 cut(s) 453
BsaJI CCNNGG 1 cut(s) 46
Bse21I CCTNAGG 1 cut(s) 675
BseDI CCNNGG 1 cut(s) 46
BseGI GGATG 2 cut(s) 596, 604
BseMII CTCAG 4 cut(s) 387, 626, 666, 751
Bsh1236I CGCG 1 cut(s) 311
BshFI GGCC 1 cut(s) 698
BshNI GGYRCC 2 cut(s) 452, 670
BslFI GGGAC 1 cut(s) 457
BsmFI GGGAC 1 cut(s) 457
BsnI GGCC 1 cut(s) 698
Bsp1407I TGTACA 1 cut(s) 364
Bsp143I GATC 4 cut(s) 331, 427, 492, 594
Bsp1720I GCTNAGC 1 cut(s) 737
BspACI CCGC 1 cut(s) 309
BspANI GGCC 1 cut(s) 698
BspCNI CTCAG 4 cut(s) 386, 625, 667, 750
BspFNI CGCG 1 cut(s) 311
BspLI GGNNCC 4 cut(s) 454, 596, 672, 699
BspPI GGATC 3 cut(s) 435, 589, 602
BspT107I GGYRCC 2 cut(s) 452, 670
BsrGI TGTACA 1 cut(s) 364
BssECI CCNNGG 1 cut(s) 46
BssMI GATC 4 cut(s) 331, 427, 492, 594
BssNI GRCGYC 1 cut(s) 453
BssT1I CCWWGG 1 cut(s) 46
Bst4CI ACNGT 4 cut(s) 220, 229, 292, 324
BstACI GRCGYC 1 cut(s) 453
BstAPI GCANNNNNTGC 1 cut(s) 171
BstAUI TGTACA 1 cut(s) 364
BstC8I GCNNGC 1 cut(s) 405
BstDEI CTNAG 6 cut(s) 101, 373, 408, 612, 675, 737
BstF5I GGATG 2 cut(s) 596, 604
BstFNI CGCG 1 cut(s) 311
BstH2I RGCGCY 1 cut(s) 456
BstHHI GCGC 1 cut(s) 455
BstKTI GATC 4 cut(s) 334, 430, 495, 597
BstMBI GATC 4 cut(s) 331, 427, 492, 594
BstMWI GCNNNNNNNGC 1 cut(s) 171
BstSFI CTRYAG 1 cut(s) 585
BstUI CGCG 1 cut(s) 311
BstX2I RGATCY 2 cut(s) 427, 594
BstXI CCANNNNNNTGG 2 cut(s) 204, 694
BstYI RGATCY 2 cut(s) 427, 594
Bsu36I CCTNAGG 1 cut(s) 675
BsuRI GGCC 1 cut(s) 698
BtrI CACGTC 1 cut(s) 659
BtsCI GGATG 2 cut(s) 596, 604
BtsIMutI CAGTG 1 cut(s) 639
Cac8I GCNNGC 1 cut(s) 405
CfoI GCGC 1 cut(s) 455
Cfr13I GGNCC 1 cut(s) 697
Csp6I GTAC 2 cut(s) 365, 671
CviAII CATG 2 cut(s) 327, 694
CviQI GTAC 2 cut(s) 365, 671
DdeI CTNAG 6 cut(s) 101, 373, 408, 612, 675, 737
DinI GGCGCC 1 cut(s) 454
DpnI GATC 4 cut(s) 333, 429, 494, 596
DpnII GATC 4 cut(s) 331, 427, 492, 594
DraIII CACNNNGTG 1 cut(s) 17
DrdI GACNNNNNNGTC 1 cut(s) 548
DseDI GACNNNNNNGTC 1 cut(s) 548
Eco130I CCWWGG 1 cut(s) 46
Eco57I CTGAAG 1 cut(s) 383
Eco81I CCTNAGG 1 cut(s) 675
EcoRI GAATTC 2 cut(s) 148, 313
EcoT14I CCWWGG 1 cut(s) 46
EgeI GGCGCC 1 cut(s) 454
EheI GGCGCC 1 cut(s) 454
ErhI CCWWGG 1 cut(s) 46
FaeI CATG 2 cut(s) 330, 697
FalI AAGNNNNNCTT 2 cut(s) 381, 413
FaqI GGGAC 1 cut(s) 457
FatI CATG 2 cut(s) 326, 693
FbaI TGATCA 2 cut(s) 331, 492
Fnu4HI GCNGC 1 cut(s) 309
FokI GGATG 2 cut(s) 591, 603
Fsp4HI GCNGC 1 cut(s) 309
FspBI CTAG 1 cut(s) 348
GlaI GCGC 1 cut(s) 454
GluI GCNGC 1 cut(s) 309
HaeII RGCGCY 1 cut(s) 456
HaeIII GGCC 1 cut(s) 698
HhaI GCGC 1 cut(s) 455
Hin1I GRCGYC 1 cut(s) 453
Hin1II CATG 2 cut(s) 330, 697
Hin6I GCGC 1 cut(s) 453
HinP1I GCGC 1 cut(s) 453
HindIII AAGCTT 1 cut(s) 103
HinfI GANTC 4 cut(s) 111, 445, 487, 509
Hpy188I TCNGA 4 cut(s) 319, 492, 615, 807
Hpy188III TCNNGA 1 cut(s) 431
HpyAV CCTTC 4 cut(s) 255, 407, 418, 618
HpyCH4III ACNGT 4 cut(s) 220, 229, 292, 324
HpyCH4IV ACGT 2 cut(s) 658, 811
HpyCH4V TGCA 2 cut(s) 157, 264
HpyF10VI GCNNNNNNNGC 1 cut(s) 171
HpyF3I CTNAG 6 cut(s) 101, 373, 408, 612, 675, 737
HpySE526I ACGT 2 cut(s) 658, 811
Hsp92I GRCGYC 1 cut(s) 453
Hsp92II CATG 2 cut(s) 330, 697
HspAI GCGC 1 cut(s) 453
KasI GGCGCC 1 cut(s) 452
KpnI GGTACC 1 cut(s) 674
Ksp22I TGATCA 2 cut(s) 331, 492
Kzo9I GATC 4 cut(s) 331, 427, 492, 594
LmnI GCTCC 1 cut(s) 199
LpnPI CCDG 8 cut(s) 147, 368, 451, 491, 515, 597, 647, 687
MaeI CTAG 1 cut(s) 348
MaeII ACGT 2 cut(s) 658, 811
MaeIII GTNAC 3 cut(s) 59, 229, 481
MalI GATC 4 cut(s) 333, 429, 494, 596
MboI GATC 4 cut(s) 331, 427, 492, 594
MboII GAAGA 1 cut(s) 401
MflI RGATCY 2 cut(s) 427, 594
MluCI AATT 6 cut(s) 52, 148, 185, 236, 300, 313
Mly113I GGCGCC 1 cut(s) 453
MlyI GAGTC 3 cut(s) 120, 439, 481
MmeI TCCRAC 1 cut(s) 543
MnlI CCTC 4 cut(s) 58, 288, 538, 670
MseI TTAA 5 cut(s) 39, 254, 383, 462, 792
MslI CAYNNNNRTG 1 cut(s) 692
MvnI CGCG 1 cut(s) 311
MwoI GCNNNNNNNGC 1 cut(s) 171
NarI GGCGCC 1 cut(s) 453
NdeII GATC 4 cut(s) 331, 427, 492, 594
NlaIII CATG 2 cut(s) 330, 697
NlaIV GGNNCC 4 cut(s) 454, 596, 672, 699
NmuCI GTSAC 2 cut(s) 59, 481
PfeI GAWTC 1 cut(s) 509
PkrI GCNGC 1 cut(s) 310
PleI GAGTC 3 cut(s) 119, 439, 481
PluTI GGCGCC 1 cut(s) 456
PpsI GAGTC 3 cut(s) 119, 439, 481
Psp1406I AACGTT 1 cut(s) 811
PspN4I GGNNCC 4 cut(s) 454, 596, 672, 699
PspPI GGNCC 1 cut(s) 697
PsuI RGATCY 2 cut(s) 427, 594
RsaI GTAC 2 cut(s) 366, 672
RsaNI GTAC 2 cut(s) 365, 671
RseI CAYNNNNRTG 1 cut(s) 692
SaqAI TTAA 5 cut(s) 39, 254, 383, 462, 792
SatI GCNGC 1 cut(s) 309
Sau3AI GATC 4 cut(s) 331, 427, 492, 594
Sau96I GGNCC 1 cut(s) 697
SchI GAGTC 3 cut(s) 120, 439, 481
SfcI CTRYAG 1 cut(s) 585
SfoI GGCGCC 1 cut(s) 454
SmiMI CAYNNNNRTG 1 cut(s) 692
SmlI CTYRAG 2 cut(s) 106, 279
SmoI CTYRAG 2 cut(s) 106, 279
Sse9I AATT 6 cut(s) 52, 148, 185, 236, 300, 313
SsiI CCGC 1 cut(s) 309
SspDI GGCGCC 1 cut(s) 452
SspI AATATT 2 cut(s) 214, 748
SspMI CTAG 1 cut(s) 348
StyI CCWWGG 1 cut(s) 46
TaaI ACNGT 4 cut(s) 220, 229, 292, 324
TaiI ACGT 2 cut(s) 661, 814
TasI AATT 6 cut(s) 52, 148, 185, 236, 300, 313
TatI WGTACW 1 cut(s) 364
TauI GCSGC 1 cut(s) 311
TfiI GAWTC 1 cut(s) 509
Tru1I TTAA 5 cut(s) 39, 254, 383, 462, 792
Tru9I TTAA 5 cut(s) 39, 254, 383, 462, 792
TscAI CASTG 1 cut(s) 639
TseFI GTSAC 2 cut(s) 59, 481
Tsp45I GTSAC 2 cut(s) 59, 481
TspDTI ATGAA 4 cut(s) 212, 483, 582, 746
TspRI CASTG 1 cut(s) 639
XapI RAATTY 3 cut(s) 148, 185, 313
XspI CTAG 1 cut(s) 348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.