RchiOBHm_Chr4g0443791

AMSH-like ubiquitin thioesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0002227
Physical Location & Seq
Reverse (-)
64795254 .. 64799006
3753 bp
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UTR
Exon/CDS
Intron
PRQ41149

Sequence Viewer

Length: 810 bp
ATGGCAGAAAGAAACCATCTAAGCTTGAGAGTCATCAATGGGGAACAAGCTACTTTGTCATCTTTGAATTCGTTGCAATCTGGCAGTAGTTGCTTGGCTCGTAAATTTCTTGCTCCAAGCGAATGGCAGAATATTACAGTTCATACTGTTACGCAATTCTCCCCTTCTCCTTTAATCTCTTGCACACAAAGTATGCCTCAAGGTGCTACTGTTTCACAAATTACAGCCGCGAATTCAGAACAGTCATGTGATCAATCAACATCATCTAGTGTCCTGCGAGATGTACATATCTCAGCTCGGTTAATGGAAGATTTCCTTCAGCTTGCTAAGGACAACACAGAGAAGGATCTTGAAACCTGTGGGACTCTTGGCGCCTTTCTTAAAAATGAAACATTCTATGTGACGACTCTGATCATACCCAAGCAGGAATCCACTTCCAACTCTTGTCAGGCAACGAATGACGAGGAAGTCTTTGCCATACAAAATGAACAATCACTTTTTCCTGTAGGATGGATCCATACACATCCTTCTCAGAGTTGTTTTATGTCATCAGTGGATTTACACACACATTATTCACGTCAGGTTATGGTACCTGAGGCTTTTGCCATTGTCATGGCCCCAACTGATACTTCAAGAAGCTATGGAATATTCCGGCTATCTGACCCTGGTGGGATGAGTGTCTTGAAAGAGTGCCAAGAGACAGGGTTCCACACTCACGAAGAAACAGGAGATGGGAGTCCCATTTACGAGCACTGCTCCAACGTCTACACAAACTCAAATCTGAGGTTTGAAATCTTTGACTTGCGTTGA

Protein Analysis

269

Amino Acids

29.77

Weight (kDa)

5.03

Isoelectric Point (pI)

69.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
JAB PF01398 92 - 199 1e-16 JAB1/Mov34/MPN/PAD-1 ubiquitin protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 467
Acc65I GGTACC 1 cut(s) 589
AccB1I GGYRCC 2 cut(s) 371, 589
AccI GTMKAC 1 cut(s) 765
AccII CGCG 1 cut(s) 230
AciI CCGC 1 cut(s) 228
AclWI GGATC 3 cut(s) 354, 508, 521
AcsI RAATTY 3 cut(s) 67, 104, 232
AcuI CTGAAG 1 cut(s) 302
AcyI GRCGYC 1 cut(s) 372
AfaI GTAC 2 cut(s) 285, 591
AgsI TTSAA 5 cut(s) 67, 353, 633, 685, 791
AjiI CACGTC 1 cut(s) 578
AjnI CCWGG 1 cut(s) 664
AjuI GAANNNNNNNTTGG 2 cut(s) 613, 645
AluBI AGCT 5 cut(s) 24, 50, 296, 322, 639
AluI AGCT 5 cut(s) 24, 50, 296, 322, 639
Alw21I GWGCWC 1 cut(s) 753
Alw26I GTCTC 1 cut(s) 692
AlwI GGATC 3 cut(s) 354, 508, 521
AoxI GGCC 1 cut(s) 615
ApoI RAATTY 3 cut(s) 67, 104, 232
Asp718I GGTACC 1 cut(s) 589
AspLEI GCGC 1 cut(s) 374
AspS9I GGNCC 1 cut(s) 616
AxyI CCTNAGG 1 cut(s) 594
BamHI GGATCC 1 cut(s) 513
BanI GGYRCC 2 cut(s) 371, 589
Bbv12I GWGCWC 1 cut(s) 753
BccI CCATC 3 cut(s) 24, 504, 725
BciT130I CCWGG 1 cut(s) 666
BclI TGATCA 2 cut(s) 250, 411
BcoDI GTCTC 1 cut(s) 692
BfaI CTAG 1 cut(s) 267
BfmI CTRYAG 1 cut(s) 504
BfoI RGCGCY 1 cut(s) 375
BisI GCNGC 1 cut(s) 228
BlsI GCNGC 1 cut(s) 229
Bme1390I CCNGG 1 cut(s) 666
BmgBI CACGTC 1 cut(s) 578
BmgT120I GGNCC 1 cut(s) 616
BmiI GGNNCC 5 cut(s) 373, 515, 591, 618, 707
BmrFI CCNGG 1 cut(s) 666
BplI GAGNNNNNCTC 2 cut(s) 740, 772
Bpu10I CCTNAGC 1 cut(s) 327
BpuEI CTTGAG 2 cut(s) 46, 183
BsaHI GRCGYC 1 cut(s) 372
BsaJI CCNNGG 1 cut(s) 664
Bse21I CCTNAGG 1 cut(s) 594
BseBI CCWGG 1 cut(s) 666
BseDI CCNNGG 1 cut(s) 664
BseGI GGATG 3 cut(s) 515, 523, 678
BseMII CTCAG 4 cut(s) 306, 545, 585, 773
Bsh1236I CGCG 1 cut(s) 230
BshFI GGCC 1 cut(s) 617
BshNI GGYRCC 2 cut(s) 371, 589
BsiHKAI GWGCWC 1 cut(s) 753
BsiSI CCGG 1 cut(s) 652
BslFI GGGAC 2 cut(s) 376, 723
BsmAI GTCTC 1 cut(s) 692
BsmFI GGGAC 2 cut(s) 376, 723
BsnI GGCC 1 cut(s) 617
Bsp1286I GDGCHC 1 cut(s) 753
Bsp1407I TGTACA 1 cut(s) 283
Bsp143I GATC 4 cut(s) 250, 346, 411, 513
BspACI CCGC 1 cut(s) 228
BspANI GGCC 1 cut(s) 617
BspCNI CTCAG 4 cut(s) 305, 544, 586, 774
BspFNI CGCG 1 cut(s) 230
BspLI GGNNCC 5 cut(s) 373, 515, 591, 618, 707
BspPI GGATC 3 cut(s) 354, 508, 521
BspT107I GGYRCC 2 cut(s) 371, 589
BsrGI TGTACA 1 cut(s) 283
BssECI CCNNGG 1 cut(s) 664
BssMI GATC 4 cut(s) 250, 346, 411, 513
BssNI GRCGYC 1 cut(s) 372
Bst2UI CCWGG 1 cut(s) 666
Bst4CI ACNGT 4 cut(s) 139, 148, 211, 243
BstACI GRCGYC 1 cut(s) 372
BstAPI GCANNNNNTGC 1 cut(s) 90
BstAUI TGTACA 1 cut(s) 283
BstC8I GCNNGC 1 cut(s) 324
BstDEI CTNAG 6 cut(s) 20, 292, 327, 531, 594, 782
BstF5I GGATG 3 cut(s) 515, 523, 678
BstFNI CGCG 1 cut(s) 230
BstH2I RGCGCY 1 cut(s) 375
BstHHI GCGC 1 cut(s) 374
BstKTI GATC 4 cut(s) 253, 349, 414, 516
BstMAI GTCTC 1 cut(s) 692
BstMBI GATC 4 cut(s) 250, 346, 411, 513
BstMWI GCNNNNNNNGC 1 cut(s) 90
BstNI CCWGG 1 cut(s) 666
BstSCI CCNGG 1 cut(s) 664
BstSFI CTRYAG 1 cut(s) 504
BstUI CGCG 1 cut(s) 230
BstX2I RGATCY 2 cut(s) 346, 513
BstXI CCANNNNNNTGG 2 cut(s) 123, 613
BstYI RGATCY 2 cut(s) 346, 513
Bsu36I CCTNAGG 1 cut(s) 594
BsuRI GGCC 1 cut(s) 617
BtrI CACGTC 1 cut(s) 578
BtsCI GGATG 3 cut(s) 515, 523, 678
BtsI GCAGTG 1 cut(s) 751
BtsIMutI CAGTG 2 cut(s) 558, 751
Cac8I GCNNGC 1 cut(s) 324
CfoI GCGC 1 cut(s) 374
Cfr13I GGNCC 1 cut(s) 616
Csp6I GTAC 2 cut(s) 284, 590
CviAII CATG 2 cut(s) 246, 613
CviQI GTAC 2 cut(s) 284, 590
DdeI CTNAG 6 cut(s) 20, 292, 327, 531, 594, 782
DinI GGCGCC 1 cut(s) 373
DpnI GATC 4 cut(s) 252, 348, 413, 515
DpnII GATC 4 cut(s) 250, 346, 411, 513
DrdI GACNNNNNNGTC 1 cut(s) 467
DseDI GACNNNNNNGTC 1 cut(s) 467
Eco57I CTGAAG 1 cut(s) 302
Eco81I CCTNAGG 1 cut(s) 594
EcoRI GAATTC 2 cut(s) 67, 232
EcoRII CCWGG 1 cut(s) 664
EgeI GGCGCC 1 cut(s) 373
EheI GGCGCC 1 cut(s) 373
FaeI CATG 2 cut(s) 249, 616
FalI AAGNNNNNCTT 2 cut(s) 300, 332
FaqI GGGAC 2 cut(s) 376, 723
FatI CATG 2 cut(s) 245, 612
FbaI TGATCA 2 cut(s) 250, 411
FblI GTMKAC 1 cut(s) 765
Fnu4HI GCNGC 1 cut(s) 228
FokI GGATG 3 cut(s) 510, 522, 685
Fsp4HI GCNGC 1 cut(s) 228
FspBI CTAG 1 cut(s) 267
GlaI GCGC 1 cut(s) 373
GluI GCNGC 1 cut(s) 228
HaeII RGCGCY 1 cut(s) 375
HaeIII GGCC 1 cut(s) 617
HapII CCGG 1 cut(s) 652
HhaI GCGC 1 cut(s) 374
Hin1I GRCGYC 1 cut(s) 372
Hin1II CATG 2 cut(s) 249, 616
Hin6I GCGC 1 cut(s) 372
HinP1I GCGC 1 cut(s) 372
HindIII AAGCTT 1 cut(s) 22
HinfI GANTC 5 cut(s) 30, 364, 406, 428, 736
HpaII CCGG 1 cut(s) 652
Hpy166II GTNNAC 1 cut(s) 766
Hpy188I TCNGA 5 cut(s) 238, 411, 534, 661, 783
Hpy188III TCNNGA 4 cut(s) 350, 633, 682, 716
Hpy8I GTNNAC 1 cut(s) 766
HpyAV CCTTC 4 cut(s) 174, 326, 337, 537
HpyCH4III ACNGT 4 cut(s) 139, 148, 211, 243
HpyCH4IV ACGT 2 cut(s) 577, 762
HpyCH4V TGCA 2 cut(s) 76, 183
HpyF10VI GCNNNNNNNGC 1 cut(s) 90
HpyF3I CTNAG 6 cut(s) 20, 292, 327, 531, 594, 782
HpySE526I ACGT 2 cut(s) 577, 762
Hsp92I GRCGYC 1 cut(s) 372
Hsp92II CATG 2 cut(s) 249, 616
HspAI GCGC 1 cut(s) 372
KasI GGCGCC 1 cut(s) 371
KpnI GGTACC 1 cut(s) 593
Ksp22I TGATCA 2 cut(s) 250, 411
Kzo9I GATC 4 cut(s) 250, 346, 411, 513
LmnI GCTCC 2 cut(s) 118, 761
MaeI CTAG 1 cut(s) 267
MaeII ACGT 2 cut(s) 577, 762
MaeIII GTNAC 2 cut(s) 148, 400
MalI GATC 4 cut(s) 252, 348, 413, 515
MboI GATC 4 cut(s) 250, 346, 411, 513
MboII GAAGA 2 cut(s) 320, 731
MflI RGATCY 2 cut(s) 346, 513
MhlI GDGCHC 1 cut(s) 753
MluCI AATT 5 cut(s) 67, 104, 155, 219, 232
Mly113I GGCGCC 1 cut(s) 372
MlyI GAGTC 4 cut(s) 39, 358, 400, 745
MmeI TCCRAC 2 cut(s) 462, 783
MnlI CCTC 4 cut(s) 207, 457, 589, 777
MseI TTAA 3 cut(s) 173, 302, 381
MslI CAYNNNNRTG 1 cut(s) 611
MspI CCGG 1 cut(s) 652
MspR9I CCNGG 1 cut(s) 666
MvaI CCWGG 1 cut(s) 666
MvnI CGCG 1 cut(s) 230
MwoI GCNNNNNNNGC 1 cut(s) 90
NarI GGCGCC 1 cut(s) 372
NdeII GATC 4 cut(s) 250, 346, 411, 513
NlaIII CATG 2 cut(s) 249, 616
NlaIV GGNNCC 5 cut(s) 373, 515, 591, 618, 707
NmuCI GTSAC 1 cut(s) 400
PfeI GAWTC 1 cut(s) 428
PkrI GCNGC 1 cut(s) 229
PleI GAGTC 4 cut(s) 38, 358, 400, 744
PluTI GGCGCC 1 cut(s) 375
PpsI GAGTC 4 cut(s) 38, 358, 400, 744
Psp6I CCWGG 1 cut(s) 664
PspGI CCWGG 1 cut(s) 664
PspN4I GGNNCC 5 cut(s) 373, 515, 591, 618, 707
PspPI GGNCC 1 cut(s) 616
PsuI RGATCY 2 cut(s) 346, 513
RsaI GTAC 2 cut(s) 285, 591
RsaNI GTAC 2 cut(s) 284, 590
RseI CAYNNNNRTG 1 cut(s) 611
SaqAI TTAA 3 cut(s) 173, 302, 381
SatI GCNGC 1 cut(s) 228
Sau3AI GATC 4 cut(s) 250, 346, 411, 513
Sau96I GGNCC 1 cut(s) 616
SchI GAGTC 4 cut(s) 39, 358, 400, 745
ScrFI CCNGG 1 cut(s) 666
SduI GDGCHC 1 cut(s) 753
SfcI CTRYAG 1 cut(s) 504
SfoI GGCGCC 1 cut(s) 373
SmiMI CAYNNNNRTG 1 cut(s) 611
SmlI CTYRAG 2 cut(s) 25, 198
SmoI CTYRAG 2 cut(s) 25, 198
Sse9I AATT 5 cut(s) 67, 104, 155, 219, 232
SsiI CCGC 1 cut(s) 228
SspDI GGCGCC 1 cut(s) 371
SspI AATATT 2 cut(s) 133, 648
SspMI CTAG 1 cut(s) 267
StyD4I CCNGG 1 cut(s) 664
TaaI ACNGT 4 cut(s) 139, 148, 211, 243
TaiI ACGT 2 cut(s) 580, 765
TasI AATT 5 cut(s) 67, 104, 155, 219, 232
TatI WGTACW 1 cut(s) 283
TauI GCSGC 1 cut(s) 230
TfiI GAWTC 1 cut(s) 428
Tru1I TTAA 3 cut(s) 173, 302, 381
Tru9I TTAA 3 cut(s) 173, 302, 381
TscAI CASTG 2 cut(s) 558, 758
TseFI GTSAC 1 cut(s) 400
Tsp45I GTSAC 1 cut(s) 400
TspDTI ATGAA 3 cut(s) 131, 402, 501
TspRI CASTG 2 cut(s) 558, 758
XapI RAATTY 3 cut(s) 67, 104, 232
XmiI GTMKAC 1 cut(s) 765
XspI CTAG 1 cut(s) 267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.