MD16G1093400.v1.1

AMSH-like ubiquitin thioesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0002227
Physical Location & Seq
Forward (+)
6457626 .. 6461618
3993 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1093400.v1.1.491

Sequence Viewer

Length: 837 bp
ATGGCAGAGAGAAGCCATATAAGCGCCAGGGGCATCGATGGGGAAAATCCTAGGTTGTCATCTTTGTGTTCAGTGGAATCTGGCAGTAGTTGCTTCGCGTGTAAATTTCCTGGTCCAAACTTTTTGAAGACAACTAGTGACTATCAGAATATCACAGTTCATTCTGTTACTCAATGCTCTCCTTCACCTGTAATCTCTTGCGTAGAAAAGCCTCAAAGTTCTAATATATCACAAATTACGGACGCAAATACAGAACATGGTCAGTCACAGTCTTCTAATAAATCAGCATCATCTGGGGTCCTGCGAGATGTACATATTTCAGCTCAGTTAATGGAAGATTTCCTTGATCTTGCTAAAGAAAACACGGAGAAGGATCTAGAAACCTGCGGGATTCTTGGCGCTTTTCTTAAAAATGGAACATTCTATGTGACAACTCTGATCATACCCAAACAGGAATCAACTTCCAGCTCTTGTCTGGCAACAAACGATGAGGAAGCTTTTGCCATTCAAAACGAACACTCACTTTTTCCGGTTGGATGGATCCATACACATCCTTCTCAAAGTTGTTTCATGTCATCAGTGGATTTGCACACTCATTATTCGTACCAGGTTATGATACCCGAGGCTATTGCCGTTGTCATGGCTCCAACTGATACCTCAAGGAGCTATGGAATATTTCGGCTATCTGATCCTGGCGGGATGAGTGTCCTGAAAGCGTGCCAGGAGCAAGGATTTCATCCTCACGGAGAAACAACAGACGGGAGTCCCGTTTTCGAGCACTGCTCTAATGTATACAAGAATTCAAATCTGAGGTTCGAAATCTTCGACTTGCGTTGA

Protein Analysis

279

Amino Acids

30.38

Weight (kDa)

5.28

Isoelectric Point (pI)

62.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
JAB PF01398 102 - 208 1e-17 JAB1/Mov34/MPN/PAD-1 ubiquitin protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 392
AccI GTMKAC 1 cut(s) 792
AccII CGCG 1 cut(s) 98
AciI CCGC 2 cut(s) 387, 696
AclWI GGATC 4 cut(s) 381, 535, 548, 683
AcsI RAATTY 2 cut(s) 104, 799
AfaI GTAC 2 cut(s) 312, 605
AgsI TTSAA 3 cut(s) 127, 509, 804
AhlI ACTAGT 1 cut(s) 134
AjnI CCWGG 5 cut(s) 26, 109, 606, 691, 720
AluBI AGCT 4 cut(s) 323, 468, 497, 666
AluI AGCT 4 cut(s) 323, 468, 497, 666
Alw21I GWGCWC 1 cut(s) 780
AlwI GGATC 4 cut(s) 381, 535, 548, 683
Ama87I CYCGRG 1 cut(s) 620
ApoI RAATTY 2 cut(s) 104, 799
AspA2I CCTAGG 1 cut(s) 50
AspLEI GCGC 2 cut(s) 26, 401
AspS9I GGNCC 2 cut(s) 113, 298
AsuHPI GGTGA 1 cut(s) 177
AsuII TTCGAA 1 cut(s) 816
AvaI CYCGRG 1 cut(s) 620
AvaII GGWCC 2 cut(s) 113, 298
AvrII CCTAGG 1 cut(s) 50
BamHI GGATCC 1 cut(s) 540
BbsI GAAGAC 2 cut(s) 134, 264
Bbv12I GWGCWC 1 cut(s) 780
BccI CCATC 2 cut(s) 32, 531
BceAI ACGGC 1 cut(s) 617
BcgI CGANNNNNNTGC 2 cut(s) 611, 645
BciT130I CCWGG 5 cut(s) 28, 111, 608, 693, 722
BclI TGATCA 1 cut(s) 438
BcuI ACTAGT 1 cut(s) 134
BfaI CTAG 3 cut(s) 51, 135, 377
BfoI RGCGCY 2 cut(s) 27, 402
BfuAI ACCTGC 1 cut(s) 392
BlnI CCTAGG 1 cut(s) 50
Bme1390I CCNGG 5 cut(s) 28, 111, 608, 693, 722
Bme18I GGWCC 2 cut(s) 113, 298
BmeT110I CYCGRG 1 cut(s) 620
BmgT120I GGNCC 2 cut(s) 113, 298
BmiI GGNNCC 3 cut(s) 299, 542, 645
BmrFI CCNGG 5 cut(s) 28, 111, 608, 693, 722
BmsI GCATC 2 cut(s) 42, 296
BoxI GACNNNNGTC 1 cut(s) 762
BpiI GAAGAC 2 cut(s) 134, 264
BplI GAGNNNNNCTC 2 cut(s) 767, 799
Bpu14I TTCGAA 1 cut(s) 816
BpuEI CTTGAG 1 cut(s) 643
Bsa29I ATCGAT 1 cut(s) 36
BsaJI CCNNGG 3 cut(s) 27, 50, 621
BsaWI WCCGGW 1 cut(s) 529
BseBI CCWGG 5 cut(s) 28, 111, 608, 693, 722
BseCI ATCGAT 1 cut(s) 36
BseDI CCNNGG 3 cut(s) 27, 50, 621
BseGI GGATG 4 cut(s) 542, 550, 705, 736
BseMII CTCAG 2 cut(s) 338, 800
Bsh1236I CGCG 1 cut(s) 98
BshVI ATCGAT 1 cut(s) 36
BsiHKAI GWGCWC 1 cut(s) 780
BsiHKCI CYCGRG 1 cut(s) 620
BsiSI CCGG 1 cut(s) 530
BslFI GGGAC 1 cut(s) 750
BsmFI GGGAC 1 cut(s) 750
BsoBI CYCGRG 1 cut(s) 620
Bsp119I TTCGAA 1 cut(s) 816
Bsp1286I GDGCHC 1 cut(s) 780
Bsp1407I TGTACA 1 cut(s) 310
Bsp143I GATC 5 cut(s) 346, 373, 438, 540, 688
BspACI CCGC 2 cut(s) 387, 696
BspCNI CTCAG 2 cut(s) 337, 801
BspDI ATCGAT 1 cut(s) 36
BspFNI CGCG 1 cut(s) 98
BspLI GGNNCC 3 cut(s) 299, 542, 645
BspMI ACCTGC 1 cut(s) 392
BspPI GGATC 4 cut(s) 381, 535, 548, 683
BspT104I TTCGAA 1 cut(s) 816
BsrGI TGTACA 1 cut(s) 310
BssECI CCNNGG 3 cut(s) 27, 50, 621
BssMI GATC 5 cut(s) 346, 373, 438, 540, 688
BssNAI GTATAC 1 cut(s) 793
BssT1I CCWWGG 1 cut(s) 50
Bst1107I GTATAC 1 cut(s) 793
Bst2UI CCWGG 5 cut(s) 28, 111, 608, 693, 722
Bst4CI ACNGT 2 cut(s) 157, 270
BstAPI GCANNNNNTGC 1 cut(s) 90
BstAUI TGTACA 1 cut(s) 310
BstBI TTCGAA 1 cut(s) 816
BstC8I GCNNGC 1 cut(s) 718
BstDEI CTNAG 2 cut(s) 324, 809
BstF5I GGATG 4 cut(s) 542, 550, 705, 736
BstFNI CGCG 1 cut(s) 98
BstH2I RGCGCY 2 cut(s) 27, 402
BstHHI GCGC 2 cut(s) 26, 401
BstKTI GATC 5 cut(s) 349, 376, 441, 543, 691
BstMBI GATC 5 cut(s) 346, 373, 438, 540, 688
BstMWI GCNNNNNNNGC 3 cut(s) 21, 30, 90
BstNI CCWGG 5 cut(s) 28, 111, 608, 693, 722
BstPAI GACNNNNGTC 1 cut(s) 762
BstSCI CCNGG 5 cut(s) 26, 109, 606, 691, 720
BstUI CGCG 1 cut(s) 98
BstV2I GAAGAC 2 cut(s) 134, 264
BstX2I RGATCY 2 cut(s) 373, 540
BstYI RGATCY 2 cut(s) 373, 540
BstZ17I GTATAC 1 cut(s) 793
Bsu15I ATCGAT 1 cut(s) 36
BsuTUI ATCGAT 1 cut(s) 36
BtsCI GGATG 4 cut(s) 542, 550, 705, 736
BtsI GCAGTG 1 cut(s) 778
BtsIMutI CAGTG 3 cut(s) 78, 585, 778
BveI ACCTGC 1 cut(s) 392
Cac8I GCNNGC 1 cut(s) 718
CfoI GCGC 2 cut(s) 26, 401
Cfr13I GGNCC 2 cut(s) 113, 298
ClaI ATCGAT 1 cut(s) 36
CseI GACGC 1 cut(s) 251
CsiI ACCWGGT 1 cut(s) 606
Csp6I GTAC 2 cut(s) 311, 604
CviAII CATG 3 cut(s) 257, 571, 640
CviJI RGCY 9 cut(s) 15, 211, 323, 468, 497, 626, 644, 666, 682
CviKI_1 RGCY 9 cut(s) 15, 211, 323, 468, 497, 626, 644, 666, 682
CviQI GTAC 2 cut(s) 311, 604
DdeI CTNAG 2 cut(s) 324, 809
DpnI GATC 5 cut(s) 348, 375, 440, 542, 690
DpnII GATC 5 cut(s) 346, 373, 438, 540, 688
Eco130I CCWWGG 1 cut(s) 50
Eco47I GGWCC 2 cut(s) 113, 298
Eco88I CYCGRG 1 cut(s) 620
EcoO109I RGGNCCY 1 cut(s) 298
EcoRI GAATTC 1 cut(s) 799
EcoRII CCWGG 5 cut(s) 26, 109, 606, 691, 720
EcoT14I CCWWGG 1 cut(s) 50
ErhI CCWWGG 1 cut(s) 50
FaeI CATG 3 cut(s) 260, 574, 643
FalI AAGNNNNNCTT 2 cut(s) 327, 359
FaqI GGGAC 1 cut(s) 750
FatI CATG 3 cut(s) 256, 570, 639
FauI CCCGC 2 cut(s) 380, 689
FbaI TGATCA 1 cut(s) 438
FblI GTMKAC 1 cut(s) 792
FokI GGATG 4 cut(s) 537, 549, 712, 723
FspBI CTAG 3 cut(s) 51, 135, 377
GlaI GCGC 2 cut(s) 25, 400
HaeII RGCGCY 2 cut(s) 27, 402
HapII CCGG 1 cut(s) 530
HgaI GACGC 1 cut(s) 251
HhaI GCGC 2 cut(s) 26, 401
Hin1II CATG 3 cut(s) 260, 574, 643
Hin6I GCGC 2 cut(s) 24, 399
HinP1I GCGC 2 cut(s) 24, 399
HindIII AAGCTT 1 cut(s) 495
HinfI GANTC 4 cut(s) 77, 391, 455, 763
HpaII CCGG 1 cut(s) 530
HphI GGTGA 1 cut(s) 177
Hpy166II GTNNAC 1 cut(s) 793
Hpy188I TCNGA 4 cut(s) 147, 438, 688, 810
Hpy188III TCNNGA 2 cut(s) 377, 709
Hpy8I GTNNAC 1 cut(s) 793
HpyAV CCTTC 3 cut(s) 192, 364, 564
HpyCH4III ACNGT 2 cut(s) 157, 270
HpyCH4V TGCA 1 cut(s) 589
HpyF10VI GCNNNNNNNGC 3 cut(s) 21, 30, 90
HpyF3I CTNAG 2 cut(s) 324, 809
Hsp92II CATG 3 cut(s) 260, 574, 643
HspAI GCGC 2 cut(s) 24, 399
Ksp22I TGATCA 1 cut(s) 438
Kzo9I GATC 5 cut(s) 346, 373, 438, 540, 688
LmnI GCTCC 3 cut(s) 649, 663, 724
LweI GCATC 2 cut(s) 42, 296
MabI ACCWGGT 1 cut(s) 606
MaeI CTAG 3 cut(s) 51, 135, 377
MaeIII GTNAC 4 cut(s) 137, 166, 264, 427
MalI GATC 5 cut(s) 348, 375, 440, 542, 690
MboI GATC 5 cut(s) 346, 373, 438, 540, 688
MboII GAAGA 4 cut(s) 139, 264, 347, 814
MflI RGATCY 2 cut(s) 373, 540
MhlI GDGCHC 1 cut(s) 780
MluCI AATT 3 cut(s) 104, 234, 799
MlyI GAGTC 1 cut(s) 772
MmeI TCCRAC 2 cut(s) 514, 671
MnlI CCTC 6 cut(s) 222, 484, 616, 667, 750, 804
MseI TTAA 2 cut(s) 329, 408
MslI CAYNNNNRTG 1 cut(s) 64
MspI CCGG 1 cut(s) 530
MspR9I CCNGG 5 cut(s) 28, 111, 608, 693, 722
MvaI CCWGG 5 cut(s) 28, 111, 608, 693, 722
MvnI CGCG 1 cut(s) 98
MwoI GCNNNNNNNGC 3 cut(s) 21, 30, 90
NdeII GATC 5 cut(s) 346, 373, 438, 540, 688
NlaIII CATG 3 cut(s) 260, 574, 643
NlaIV GGNNCC 3 cut(s) 299, 542, 645
NmuCI GTSAC 3 cut(s) 137, 264, 427
NspV TTCGAA 1 cut(s) 816
PcsI WCGNNNNNNNCGW 2 cut(s) 765, 822
PfeI GAWTC 3 cut(s) 77, 391, 455
PleI GAGTC 1 cut(s) 771
PpsI GAGTC 1 cut(s) 771
PpuMI RGGWCCY 1 cut(s) 298
PshAI GACNNNNGTC 1 cut(s) 762
Psp5II RGGWCCY 1 cut(s) 298
Psp6I CCWGG 5 cut(s) 26, 109, 606, 691, 720
PspGI CCWGG 5 cut(s) 26, 109, 606, 691, 720
PspN4I GGNNCC 3 cut(s) 299, 542, 645
PspPI GGNCC 2 cut(s) 113, 298
PspPPI RGGWCCY 1 cut(s) 298
PsuI RGATCY 2 cut(s) 373, 540
RsaI GTAC 2 cut(s) 312, 605
RsaNI GTAC 2 cut(s) 311, 604
RseI CAYNNNNRTG 1 cut(s) 64
SaqAI TTAA 2 cut(s) 329, 408
Sau3AI GATC 5 cut(s) 346, 373, 438, 540, 688
Sau96I GGNCC 2 cut(s) 113, 298
SchI GAGTC 1 cut(s) 772
ScrFI CCNGG 5 cut(s) 28, 111, 608, 693, 722
SduI GDGCHC 1 cut(s) 780
SexAI ACCWGGT 1 cut(s) 606
SfaNI GCATC 2 cut(s) 42, 296
SfuI TTCGAA 1 cut(s) 816
SinI GGWCC 2 cut(s) 113, 298
SmiMI CAYNNNNRTG 1 cut(s) 64
SmlI CTYRAG 1 cut(s) 658
SmoI CTYRAG 1 cut(s) 658
SpeI ACTAGT 1 cut(s) 134
Sse9I AATT 3 cut(s) 104, 234, 799
SsiI CCGC 2 cut(s) 387, 696
SspI AATATT 1 cut(s) 675
SspMI CTAG 3 cut(s) 51, 135, 377
StyD4I CCNGG 5 cut(s) 26, 109, 606, 691, 720
StyI CCWWGG 1 cut(s) 50
TaaI ACNGT 2 cut(s) 157, 270
TaqI TCGA 4 cut(s) 36, 774, 816, 825
TasI AATT 3 cut(s) 104, 234, 799
TatI WGTACW 1 cut(s) 310
TfiI GAWTC 3 cut(s) 77, 391, 455
Tru1I TTAA 2 cut(s) 329, 408
Tru9I TTAA 2 cut(s) 329, 408
TscAI CASTG 3 cut(s) 78, 585, 785
TseFI GTSAC 3 cut(s) 137, 264, 427
Tsp45I GTSAC 3 cut(s) 137, 264, 427
TspDTI ATGAA 3 cut(s) 149, 559, 725
TspGWI ACGGA 3 cut(s) 254, 380, 759
TspRI CASTG 3 cut(s) 78, 585, 785
VpaK11BI GGWCC 2 cut(s) 113, 298
XapI RAATTY 2 cut(s) 104, 799
XbaI TCTAGA 1 cut(s) 376
XcmI CCANNNNNNNNNTGG 1 cut(s) 472
XmaJI CCTAGG 1 cut(s) 50
XmiI GTMKAC 1 cut(s) 792
XspI CTAG 3 cut(s) 51, 135, 377
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.