FvH4_4g34960

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0016321
Physical Location & Seq
Reverse (-)
32453852 .. 32456628
2777 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g34960.t1

Sequence Viewer

Length: 513 bp
ATGGCACTGCACGGCTCCTGTGGGCCTCTAATTTCGAGCCCATTACTCACTCCCACCACAACTCCAACCTTGACCATTCTCATCCATCCACGCCCACCACCTGTTCGTGATAATGCCAGACTCAGAACCACCCCCACATTGCCGCTAAGAGCCACCGCCACCACACCCCTTAAGAATCAATACGTGTACCCGGACCCAGTTCCGGAATTTGCAGAATGCGAGACCCACAAGTTCAGAGCTGAGCTTTCCAGGAAGCTATTGAAGGACAAGGCGACTTTTGGGGACGACTTCAATAGTGTCGTTGACGTATGCGCTGAGATATTTAGTGAATTCTTGCTCAATGAGTATGGAGGACCTGGAACATTGGTAGTGGATCCCTTCACTGATATGCTGATTGCTCTCAAGGAGAAGGAACTACCTGGAGCACCTTTGGCTGCAAGGGCATCACTGTTATGGGCTCAAAACTATTTGGATCAAGACTGGGATGCTTGGAACACGAAATCATCAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

171

Amino Acids

18.82

Weight (kDa)

5.6

Isoelectric Point (pI)

36.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016321)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g34960
malus_domestica MD13G1087300.v1.1 MD16G1087800.v1.1
prunus_persica Prupe.1G258900_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0444421
rosa_laevigata RLG00000005831
rosa_roxburghii Rroxscaffold_5G00384950
rosa_rugosa Rorug04G0353000
rosa_samantha Rh4AG413000 Rh4AG413100 Rh4BG424500 Rh4BG424600 Rh4CG438900 Rh4DG419900
rosa_wichuraiana Rw4G035480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 202
AciI CCGC 2 cut(s) 143, 156
AclWI GGATC 3 cut(s) 368, 381, 480
AcsI RAATTY 2 cut(s) 206, 329
AfaI GTAC 1 cut(s) 188
AfiI CCNNNNNNNGG 1 cut(s) 202
AflII CTTAAG 1 cut(s) 170
AflIII ACRYGT 1 cut(s) 183
AgsI TTSAA 2 cut(s) 262, 292
AjnI CCWGG 3 cut(s) 248, 355, 418
AluBI AGCT 3 cut(s) 239, 244, 256
AluI AGCT 3 cut(s) 239, 244, 256
Alw21I GWGCWC 1 cut(s) 427
Alw26I GTCTC 1 cut(s) 215
AlwI GGATC 3 cut(s) 368, 381, 480
Aor13HI TCCGGA 1 cut(s) 202
AoxI GGCC 1 cut(s) 23
ApeKI GCWGC 1 cut(s) 434
ApoI RAATTY 2 cut(s) 206, 329
AspLEI GCGC 1 cut(s) 314
AspS9I GGNCC 3 cut(s) 23, 193, 353
AsuC2I CCSGG 1 cut(s) 191
AvaII GGWCC 2 cut(s) 193, 353
BamHI GGATCC 1 cut(s) 373
BanII GRGCYC 2 cut(s) 41, 460
Bbv12I GWGCWC 1 cut(s) 427
BbvI GCAGC 1 cut(s) 421
BccI CCATC 1 cut(s) 93
BceAI ACGGC 1 cut(s) 28
BciT130I CCWGG 3 cut(s) 250, 357, 420
BcnI CCSGG 1 cut(s) 191
BcoDI GTCTC 1 cut(s) 215
BfrI CTTAAG 1 cut(s) 170
BisI GCNGC 2 cut(s) 143, 435
BlpI GCTNAGC 1 cut(s) 240
BlsI GCNGC 2 cut(s) 144, 436
Bme1390I CCNGG 4 cut(s) 191, 250, 357, 420
Bme18I GGWCC 2 cut(s) 193, 353
BmgT120I GGNCC 3 cut(s) 23, 193, 353
BmiI GGNNCC 3 cut(s) 16, 195, 375
BmrFI CCNGG 4 cut(s) 191, 250, 357, 420
BmrI ACTGGG 2 cut(s) 191, 490
BmsI GCATC 2 cut(s) 452, 475
BmuI ACTGGG 2 cut(s) 191, 490
BpmI CTGGAG 1 cut(s) 441
Bpu1102I GCTNAGC 1 cut(s) 240
BpuEI CTTGAG 1 cut(s) 386
BpuMI CCSGG 1 cut(s) 191
BsaAI YACGTR 1 cut(s) 184
BsaI GGTCTC 1 cut(s) 215
BsaWI WCCGGW 1 cut(s) 202
BsaXI ACNNNNNCTCC 2 cut(s) 46, 76
Bsc4I CCNNNNNNNGG 1 cut(s) 202
Bse1I ACTGG 2 cut(s) 197, 485
Bse3DI GCAATG 1 cut(s) 137
BseAI TCCGGA 1 cut(s) 202
BseBI CCWGG 3 cut(s) 250, 357, 420
BseGI GGATG 3 cut(s) 81, 85, 490
BseLI CCNNNNNNNGG 1 cut(s) 202
BseMI GCAATG 1 cut(s) 137
BseMII CTCAG 3 cut(s) 136, 231, 306
BseNI ACTGG 2 cut(s) 197, 485
BseXI GCAGC 1 cut(s) 421
BshFI GGCC 1 cut(s) 25
BsiHKAI GWGCWC 1 cut(s) 427
BsiSI CCGG 2 cut(s) 191, 203
BslFI GGGAC 1 cut(s) 296
BslI CCNNNNNNNGG 1 cut(s) 202
BsmAI GTCTC 1 cut(s) 215
BsmFI GGGAC 1 cut(s) 296
BsmI GAATGC 1 cut(s) 221
BsnI GGCC 1 cut(s) 25
Bso31I GGTCTC 1 cut(s) 215
Bsp1286I GDGCHC 3 cut(s) 41, 427, 460
Bsp13I TCCGGA 1 cut(s) 202
Bsp143I GATC 2 cut(s) 373, 472
Bsp1720I GCTNAGC 1 cut(s) 240
BspACI CCGC 2 cut(s) 143, 156
BspANI GGCC 1 cut(s) 25
BspCNI CTCAG 3 cut(s) 135, 232, 307
BspEI TCCGGA 1 cut(s) 202
BspLI GGNNCC 3 cut(s) 16, 195, 375
BspPI GGATC 3 cut(s) 368, 381, 480
BspTI CTTAAG 1 cut(s) 170
BspTNI GGTCTC 1 cut(s) 215
BsrDI GCAATG 1 cut(s) 137
BsrI ACTGG 2 cut(s) 197, 485
BssMI GATC 2 cut(s) 373, 472
Bst2UI CCWGG 3 cut(s) 250, 357, 420
Bst4CI ACNGT 1 cut(s) 450
BstAFI CTTAAG 1 cut(s) 170
BstBAI YACGTR 1 cut(s) 184
BstDEI CTNAG 4 cut(s) 122, 146, 240, 315
BstF5I GGATG 3 cut(s) 81, 85, 490
BstHHI GCGC 1 cut(s) 314
BstKTI GATC 2 cut(s) 376, 475
BstMAI GTCTC 1 cut(s) 215
BstMBI GATC 2 cut(s) 373, 472
BstMWI GCNNNNNNNGC 2 cut(s) 431, 440
BstNI CCWGG 3 cut(s) 250, 357, 420
BstSCI CCNGG 4 cut(s) 189, 248, 355, 418
BstV1I GCAGC 1 cut(s) 421
BstX2I RGATCY 1 cut(s) 373
BstYI RGATCY 1 cut(s) 373
BsuRI GGCC 1 cut(s) 25
BtsCI GGATG 3 cut(s) 81, 85, 490
BtsI GCAGTG 1 cut(s) 5
BtsIMutI CAGTG 3 cut(s) 5, 381, 446
CfoI GCGC 1 cut(s) 314
Cfr13I GGNCC 3 cut(s) 23, 193, 353
Csp6I GTAC 1 cut(s) 187
CviJI RGCY 9 cut(s) 15, 25, 39, 152, 239, 244, 256, 434, 458
CviKI_1 RGCY 9 cut(s) 15, 25, 39, 152, 239, 244, 256, 434, 458
CviQI GTAC 1 cut(s) 187
DdeI CTNAG 4 cut(s) 122, 146, 240, 315
DpnI GATC 2 cut(s) 375, 474
DpnII GATC 2 cut(s) 373, 472
Eco24I GRGCYC 2 cut(s) 41, 460
Eco31I GGTCTC 1 cut(s) 215
Eco47I GGWCC 2 cut(s) 193, 353
EcoO109I RGGNCCY 1 cut(s) 353
EcoRI GAATTC 1 cut(s) 329
EcoRII CCWGG 3 cut(s) 248, 355, 418
EcoT38I GRGCYC 2 cut(s) 41, 460
FaiI YATR 4 cut(s) 310, 348, 389, 454
FaqI GGGAC 1 cut(s) 296
Fnu4HI GCNGC 2 cut(s) 143, 435
FokI GGATG 3 cut(s) 68, 72, 497
FriOI GRGCYC 2 cut(s) 41, 460
Fsp4HI GCNGC 2 cut(s) 143, 435
GlaI GCGC 1 cut(s) 313
GluI GCNGC 2 cut(s) 143, 435
GsuI CTGGAG 1 cut(s) 441
HaeIII GGCC 1 cut(s) 25
HapII CCGG 2 cut(s) 191, 203
HhaI GCGC 1 cut(s) 314
Hin6I GCGC 1 cut(s) 312
HinP1I GCGC 1 cut(s) 312
HincII GTYRAC 1 cut(s) 304
HindII GTYRAC 1 cut(s) 304
HinfI GANTC 2 cut(s) 120, 175
HpaII CCGG 2 cut(s) 191, 203
Hpy166II GTNNAC 2 cut(s) 187, 304
Hpy188I TCNGA 2 cut(s) 125, 236
Hpy188III TCNNGA 3 cut(s) 107, 203, 476
Hpy8I GTNNAC 2 cut(s) 187, 304
HpyAV CCTTC 3 cut(s) 256, 388, 403
HpyCH4III ACNGT 1 cut(s) 450
HpyCH4IV ACGT 2 cut(s) 183, 306
HpyCH4V TGCA 3 cut(s) 10, 212, 437
HpyF10VI GCNNNNNNNGC 2 cut(s) 431, 440
HpyF3I CTNAG 4 cut(s) 122, 146, 240, 315
HpySE526I ACGT 2 cut(s) 183, 306
HspAI GCGC 1 cut(s) 312
Kpn2I TCCGGA 1 cut(s) 202
Kzo9I GATC 2 cut(s) 373, 472
LmnI GCTCC 2 cut(s) 20, 422
Lsp1109I GCAGC 1 cut(s) 421
LweI GCATC 2 cut(s) 452, 475
MaeII ACGT 2 cut(s) 183, 306
MalI GATC 2 cut(s) 375, 474
MboI GATC 2 cut(s) 373, 472
MflI RGATCY 1 cut(s) 373
MhlI GDGCHC 3 cut(s) 41, 427, 460
MluCI AATT 3 cut(s) 30, 206, 329
MlyI GAGTC 1 cut(s) 114
MmeI TCCRAC 1 cut(s) 89
MnlI CCTC 2 cut(s) 36, 344
MroI TCCGGA 1 cut(s) 202
MseI TTAA 1 cut(s) 171
MslI CAYNNNNRTG 3 cut(s) 386, 451, 508
MspCI CTTAAG 1 cut(s) 170
MspI CCGG 2 cut(s) 191, 203
MspR9I CCNGG 4 cut(s) 191, 250, 357, 420
Mva1269I GAATGC 1 cut(s) 221
MvaI CCWGG 3 cut(s) 250, 357, 420
MwoI GCNNNNNNNGC 2 cut(s) 431, 440
NciI CCSGG 1 cut(s) 191
NdeII GATC 2 cut(s) 373, 472
NlaIV GGNNCC 3 cut(s) 16, 195, 375
PctI GAATGC 1 cut(s) 221
PfeI GAWTC 1 cut(s) 175
PfoI TCCNGGA 1 cut(s) 248
PkrI GCNGC 2 cut(s) 144, 436
PleI GAGTC 1 cut(s) 114
PpsI GAGTC 1 cut(s) 114
Ppu21I YACGTR 1 cut(s) 184
PpuMI RGGWCCY 1 cut(s) 353
Psp5II RGGWCCY 1 cut(s) 353
Psp6I CCWGG 3 cut(s) 248, 355, 418
PspGI CCWGG 3 cut(s) 248, 355, 418
PspN4I GGNNCC 3 cut(s) 16, 195, 375
PspPI GGNCC 3 cut(s) 23, 193, 353
PspPPI RGGWCCY 1 cut(s) 353
PsuI RGATCY 1 cut(s) 373
RsaI GTAC 1 cut(s) 188
RsaNI GTAC 1 cut(s) 187
RseI CAYNNNNRTG 3 cut(s) 386, 451, 508
SaqAI TTAA 1 cut(s) 171
SatI GCNGC 2 cut(s) 143, 435
Sau3AI GATC 2 cut(s) 373, 472
Sau96I GGNCC 3 cut(s) 23, 193, 353
SchI GAGTC 1 cut(s) 114
ScrFI CCNGG 4 cut(s) 191, 250, 357, 420
SduI GDGCHC 3 cut(s) 41, 427, 460
SfaNI GCATC 2 cut(s) 452, 475
SinI GGWCC 2 cut(s) 193, 353
SmiMI CAYNNNNRTG 3 cut(s) 386, 451, 508
SmlI CTYRAG 2 cut(s) 170, 401
SmoI CTYRAG 2 cut(s) 170, 401
Sse9I AATT 3 cut(s) 30, 206, 329
SsiI CCGC 2 cut(s) 143, 156
StyD4I CCNGG 4 cut(s) 189, 248, 355, 418
TaaI ACNGT 1 cut(s) 450
TaiI ACGT 2 cut(s) 186, 309
TaqI TCGA 1 cut(s) 35
TasI AATT 3 cut(s) 30, 206, 329
TauI GCSGC 1 cut(s) 145
TfiI GAWTC 1 cut(s) 175
Tru1I TTAA 1 cut(s) 171
Tru9I TTAA 1 cut(s) 171
TscAI CASTG 3 cut(s) 12, 388, 453
TseI GCWGC 1 cut(s) 434
TspRI CASTG 3 cut(s) 12, 388, 453
Vha464I CTTAAG 1 cut(s) 170
VpaK11BI GGWCC 2 cut(s) 193, 353
XapI RAATTY 2 cut(s) 206, 329
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.