Rw4G035480

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0016321
Physical Location & Seq
Reverse (-)
62280366 .. 62282336
1971 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G035480.1

Sequence Viewer

Length: 468 bp
ATGGCAATGCACCGCTCCTGTGGGCCTCTCATTTCGAGCCCATTACTGACTCCCGCCACAACTCCAACCTCGACCATTCCCATTCATTCATGCCCGCCAACTCCACTAAGAGCCACCGCCAGCACACCCCAGAAATACTTGTACCCGGACCCAATTCCGGAATTTGCAGAATCCGAGACCCACAAGTTCAGAGCTGAGCTTTCCAGGAAGCTATTGAAGGACAAGGCGACTTTTGAGGATGACTTCAGTAGTGTCATTGACGTATGCGCCGAGATATTTAGTGAATTCTTGCTCAAGCAGTATGGAGGACCTGGAACATTGTTGGTGGATCCCTTCACTGATATGTTAGTTGCTCTCAAGGAGAAGGAACTACCTGGAGCACCTTTGGCTGCAAGGGCAACACTGTTATGGGCTCAAAACTATTTGGATCAAGACTGGGATGTTTGGAATTCGAAATCGTCAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

155

Amino Acids

17.18

Weight (kDa)

5.33

Isoelectric Point (pI)

48.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016321)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g34960
malus_domestica MD13G1087300.v1.1 MD16G1087800.v1.1
prunus_persica Prupe.1G258900_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0444421
rosa_laevigata RLG00000005831
rosa_roxburghii Rroxscaffold_5G00384950
rosa_rugosa Rorug04G0353000
rosa_samantha Rh4AG413000 Rh4AG413100 Rh4BG424500 Rh4BG424600 Rh4CG438900 Rh4DG419900
rosa_wichuraiana Rw4G035480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 15
AccIII TCCGGA 1 cut(s) 157
AciI CCGC 4 cut(s) 13, 54, 95, 117
AclWI GGATC 3 cut(s) 323, 336, 435
AcsI RAATTY 3 cut(s) 161, 284, 448
AcuI CTGAAG 1 cut(s) 229
AfaI GTAC 1 cut(s) 143
AfiI CCNNNNNNNGG 1 cut(s) 157
AgsI TTSAA 1 cut(s) 217
AjnI CCWGG 3 cut(s) 203, 310, 373
AluBI AGCT 3 cut(s) 194, 199, 211
AluI AGCT 3 cut(s) 194, 199, 211
Alw21I GWGCWC 1 cut(s) 382
Alw26I GTCTC 1 cut(s) 170
AlwI GGATC 3 cut(s) 323, 336, 435
Aor13HI TCCGGA 1 cut(s) 157
AoxI GGCC 1 cut(s) 23
ApeKI GCWGC 1 cut(s) 389
ApoI RAATTY 3 cut(s) 161, 284, 448
AspLEI GCGC 1 cut(s) 269
AspS9I GGNCC 3 cut(s) 23, 148, 308
AsuC2I CCSGG 1 cut(s) 146
AsuII TTCGAA 1 cut(s) 452
AvaII GGWCC 2 cut(s) 148, 308
BamHI GGATCC 1 cut(s) 328
BanII GRGCYC 2 cut(s) 41, 415
Bbv12I GWGCWC 1 cut(s) 382
BbvI GCAGC 1 cut(s) 376
BciT130I CCWGG 3 cut(s) 205, 312, 375
BcnI CCSGG 1 cut(s) 146
BcoDI GTCTC 1 cut(s) 170
BisI GCNGC 1 cut(s) 390
BlpI GCTNAGC 1 cut(s) 195
BlsI GCNGC 1 cut(s) 391
Bme1390I CCNGG 4 cut(s) 146, 205, 312, 375
Bme18I GGWCC 2 cut(s) 148, 308
BmgT120I GGNCC 3 cut(s) 23, 148, 308
BmiI GGNNCC 2 cut(s) 150, 330
BmrFI CCNGG 4 cut(s) 146, 205, 312, 375
BmrI ACTGGG 1 cut(s) 445
BmuI ACTGGG 1 cut(s) 445
BpmI CTGGAG 1 cut(s) 396
Bpu1102I GCTNAGC 1 cut(s) 195
Bpu14I TTCGAA 1 cut(s) 452
BpuEI CTTGAG 2 cut(s) 278, 341
BpuMI CCSGG 1 cut(s) 146
BsaI GGTCTC 1 cut(s) 170
BsaWI WCCGGW 1 cut(s) 157
Bsc4I CCNNNNNNNGG 1 cut(s) 157
Bse1I ACTGG 1 cut(s) 440
Bse3DI GCAATG 1 cut(s) 12
BseAI TCCGGA 1 cut(s) 157
BseBI CCWGG 3 cut(s) 205, 312, 375
BseGI GGATG 2 cut(s) 244, 445
BseLI CCNNNNNNNGG 1 cut(s) 157
BseMI GCAATG 1 cut(s) 12
BseMII CTCAG 1 cut(s) 186
BseNI ACTGG 1 cut(s) 440
BseXI GCAGC 1 cut(s) 376
BshFI GGCC 1 cut(s) 25
BsiHKAI GWGCWC 1 cut(s) 382
BsiSI CCGG 2 cut(s) 146, 158
BslI CCNNNNNNNGG 1 cut(s) 157
BsmAI GTCTC 1 cut(s) 170
BsnI GGCC 1 cut(s) 25
Bso31I GGTCTC 1 cut(s) 170
Bsp119I TTCGAA 1 cut(s) 452
Bsp1286I GDGCHC 3 cut(s) 41, 382, 415
Bsp13I TCCGGA 1 cut(s) 157
Bsp143I GATC 2 cut(s) 328, 427
Bsp1720I GCTNAGC 1 cut(s) 195
BspACI CCGC 4 cut(s) 13, 54, 95, 117
BspANI GGCC 1 cut(s) 25
BspCNI CTCAG 1 cut(s) 187
BspEI TCCGGA 1 cut(s) 157
BspLI GGNNCC 2 cut(s) 150, 330
BspPI GGATC 3 cut(s) 323, 336, 435
BspT104I TTCGAA 1 cut(s) 452
BspTNI GGTCTC 1 cut(s) 170
BsrBI CCGCTC 1 cut(s) 15
BsrDI GCAATG 1 cut(s) 12
BsrI ACTGG 1 cut(s) 440
BssMI GATC 2 cut(s) 328, 427
Bst2UI CCWGG 3 cut(s) 205, 312, 375
Bst4CI ACNGT 1 cut(s) 405
BstBI TTCGAA 1 cut(s) 452
BstC8I GCNNGC 2 cut(s) 95, 121
BstDEI CTNAG 2 cut(s) 107, 195
BstF5I GGATG 2 cut(s) 244, 445
BstHHI GCGC 1 cut(s) 269
BstKTI GATC 2 cut(s) 331, 430
BstMAI GTCTC 1 cut(s) 170
BstMBI GATC 2 cut(s) 328, 427
BstMWI GCNNNNNNNGC 2 cut(s) 386, 395
BstNI CCWGG 3 cut(s) 205, 312, 375
BstSCI CCNGG 4 cut(s) 144, 203, 310, 373
BstV1I GCAGC 1 cut(s) 376
BstX2I RGATCY 1 cut(s) 328
BstYI RGATCY 1 cut(s) 328
BsuRI GGCC 1 cut(s) 25
BtsCI GGATG 2 cut(s) 244, 445
BtsIMutI CAGTG 2 cut(s) 336, 401
Cac8I GCNNGC 2 cut(s) 95, 121
CfoI GCGC 1 cut(s) 269
Cfr13I GGNCC 3 cut(s) 23, 148, 308
Csp6I GTAC 1 cut(s) 142
CviAII CATG 1 cut(s) 90
CviJI RGCY 8 cut(s) 25, 39, 113, 194, 199, 211, 389, 413
CviKI_1 RGCY 8 cut(s) 25, 39, 113, 194, 199, 211, 389, 413
CviQI GTAC 1 cut(s) 142
DdeI CTNAG 2 cut(s) 107, 195
DpnI GATC 2 cut(s) 330, 429
DpnII GATC 2 cut(s) 328, 427
Eco24I GRGCYC 2 cut(s) 41, 415
Eco31I GGTCTC 1 cut(s) 170
Eco47I GGWCC 2 cut(s) 148, 308
Eco57I CTGAAG 1 cut(s) 229
EcoO109I RGGNCCY 1 cut(s) 308
EcoRI GAATTC 2 cut(s) 284, 448
EcoRII CCWGG 3 cut(s) 203, 310, 373
EcoT38I GRGCYC 2 cut(s) 41, 415
FaeI CATG 1 cut(s) 93
FaiI YATR 5 cut(s) 91, 265, 303, 344, 409
FatI CATG 1 cut(s) 89
FauI CCCGC 2 cut(s) 61, 102
Fnu4HI GCNGC 1 cut(s) 390
FokI GGATG 2 cut(s) 251, 452
FriOI GRGCYC 2 cut(s) 41, 415
Fsp4HI GCNGC 1 cut(s) 390
GlaI GCGC 1 cut(s) 268
GluI GCNGC 1 cut(s) 390
GsuI CTGGAG 1 cut(s) 396
HaeIII GGCC 1 cut(s) 25
HapII CCGG 2 cut(s) 146, 158
HhaI GCGC 1 cut(s) 269
Hin1II CATG 1 cut(s) 93
Hin6I GCGC 1 cut(s) 267
HinP1I GCGC 1 cut(s) 267
HinfI GANTC 2 cut(s) 49, 170
HpaII CCGG 2 cut(s) 146, 158
Hpy188I TCNGA 2 cut(s) 175, 191
Hpy188III TCNNGA 2 cut(s) 158, 431
HpyAV CCTTC 3 cut(s) 211, 343, 358
HpyCH4III ACNGT 1 cut(s) 405
HpyCH4IV ACGT 1 cut(s) 261
HpyCH4V TGCA 3 cut(s) 10, 167, 392
HpyF10VI GCNNNNNNNGC 2 cut(s) 386, 395
HpyF3I CTNAG 2 cut(s) 107, 195
HpySE526I ACGT 1 cut(s) 261
Hsp92II CATG 1 cut(s) 93
HspAI GCGC 1 cut(s) 267
Kpn2I TCCGGA 1 cut(s) 157
Kzo9I GATC 2 cut(s) 328, 427
LmnI GCTCC 2 cut(s) 20, 377
Lsp1109I GCAGC 1 cut(s) 376
MaeII ACGT 1 cut(s) 261
MalI GATC 2 cut(s) 330, 429
MbiI CCGCTC 1 cut(s) 15
MboI GATC 2 cut(s) 328, 427
MflI RGATCY 1 cut(s) 328
MhlI GDGCHC 3 cut(s) 41, 382, 415
MluCI AATT 4 cut(s) 153, 161, 284, 448
MlyI GAGTC 1 cut(s) 43
MmeI TCCRAC 1 cut(s) 89
MnlI CCTC 4 cut(s) 36, 79, 229, 299
MroI TCCGGA 1 cut(s) 157
MslI CAYNNNNRTG 2 cut(s) 341, 406
MspI CCGG 2 cut(s) 146, 158
MspR9I CCNGG 4 cut(s) 146, 205, 312, 375
MvaI CCWGG 3 cut(s) 205, 312, 375
MwoI GCNNNNNNNGC 2 cut(s) 386, 395
NciI CCSGG 1 cut(s) 146
NdeII GATC 2 cut(s) 328, 427
NlaIII CATG 1 cut(s) 93
NlaIV GGNNCC 2 cut(s) 150, 330
NmeAIII GCCGAG 1 cut(s) 295
NspV TTCGAA 1 cut(s) 452
PcsI WCGNNNNNNNCGW 1 cut(s) 267
PfeI GAWTC 1 cut(s) 170
PfoI TCCNGGA 1 cut(s) 203
PkrI GCNGC 1 cut(s) 391
PleI GAGTC 1 cut(s) 43
PpsI GAGTC 1 cut(s) 43
PpuMI RGGWCCY 1 cut(s) 308
Psp5II RGGWCCY 1 cut(s) 308
Psp6I CCWGG 3 cut(s) 203, 310, 373
PspGI CCWGG 3 cut(s) 203, 310, 373
PspN4I GGNNCC 2 cut(s) 150, 330
PspPI GGNCC 3 cut(s) 23, 148, 308
PspPPI RGGWCCY 1 cut(s) 308
PsuI RGATCY 1 cut(s) 328
RsaI GTAC 1 cut(s) 143
RsaNI GTAC 1 cut(s) 142
RseI CAYNNNNRTG 2 cut(s) 341, 406
SatI GCNGC 1 cut(s) 390
Sau3AI GATC 2 cut(s) 328, 427
Sau96I GGNCC 3 cut(s) 23, 148, 308
SchI GAGTC 1 cut(s) 43
ScrFI CCNGG 4 cut(s) 146, 205, 312, 375
SduI GDGCHC 3 cut(s) 41, 382, 415
SetI ASST 8 cut(s) 71, 196, 201, 213, 264, 313, 376, 385
SfuI TTCGAA 1 cut(s) 452
SinI GGWCC 2 cut(s) 148, 308
SmiMI CAYNNNNRTG 2 cut(s) 341, 406
SmlI CTYRAG 2 cut(s) 293, 356
SmoI CTYRAG 2 cut(s) 293, 356
Sse9I AATT 4 cut(s) 153, 161, 284, 448
SsiI CCGC 4 cut(s) 13, 54, 95, 117
StyD4I CCNGG 4 cut(s) 144, 203, 310, 373
TaaI ACNGT 1 cut(s) 405
TaiI ACGT 1 cut(s) 264
TaqI TCGA 3 cut(s) 35, 71, 452
TasI AATT 4 cut(s) 153, 161, 284, 448
TfiI GAWTC 1 cut(s) 170
TscAI CASTG 2 cut(s) 343, 408
TseI GCWGC 1 cut(s) 389
TspDTI ATGAA 2 cut(s) 74, 78
TspRI CASTG 2 cut(s) 343, 408
VpaK11BI GGWCC 2 cut(s) 148, 308
XapI RAATTY 3 cut(s) 161, 284, 448
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.