Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb4

Orthogroup ID

OG0006001

Physical Location & Seq

Genomic Coordinates Forward (+)

32991837 .. 32994559

Gene Structure 2723 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_4g35820.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2178 bp

ATGCTGATCTCCTATAGCTACTTCTTATATATAACTGCCCAAACTACCTCTCTTTCTCTCCATTCTTGCTTATCGGTTTCGATGTCGTCGCATAATCTGGACCTGAGTGGGAGCAAGAAGAGGAAGAGAGGCGAGAGGGTTTTCAGGTTCAAGACGTTTGGAGAAAACGGGTACCCGGTTGGGTTTGTCGCCTCTTCGTTTAGGGAAAACGTGAAAGCGCTTCTCGAATATGGGCATCTGGAGAGTACCCTATGCAATAGTGCTTCTGGGATGAAGAGCTGGTCGTTCCAGCTCCAGCTTCATCGCAATGCTCCTGTGCATCTCGTTCTCTTCGTCGTTGAAGAACCCTTTGAGGCCTCACTCAACAGTCATTGCAAGCACTGCCAATACGTAGGCTGGGGGAAGCATATGGTTTGCAATAAGAAGTACCACTTTGTGTTGCCTTCAACGGACACGATGGTGGCGGCAGCTTGTTTGGTCAACTCCAAAACTGGTGGAGGAAATAACAACAACTACGTTGATACTCCGGCGGGTAAGTCGTCGTCGTCATTGAATTTAGTGGAAGTAGAGGGGCATAACATGCATGGTGTATTTCACTCTAACGGCTTCGGGCATTTGCTTTGTGTCAATGGAGCGGAAACGGGTTCGGACTTGCCTGGACACCAGATACTTGAGTTTTGGGATCGATTGTGCACAGGATTACGTGCAAGGAAAGTGAGTTTAAACGACGTTTCACAGAAGAGAGGCATGGAGTTGAGGCTCATCCACGGAGTAGCATACAGTGAGCCCTGGTTTGGTCGTTGGGGTTATAAATTTGGCCGTGGAACATTTTGTGTCACTCAACAAATGTACCAAAGCGCCATAGAAGCATTACAAGGCATTCCCTTGTGCTTACTCATCCACCATCTTGCTGGCATTACTTGCAACCAATTAGGCCATGATATTTCCCTCATCTTCTCAAGGTATCACGCATTGTCGGATCATTCATTGGTGACTCTCGGTGATCTATTCCATTTCATGCTAGAGCTCAAGTCCCGGCTACCGACGGAAGATCAAAACTCGTGCATTGATTCCTATAACCCGGGAATGCTGGTGGAGACATCTTGCAGATGGTCTCCTAAAAGGGTTGAAATGGCGTCTCGAGTGATTGTGGAAGCCTTAAAAAGGGCTCATTTCAGATGGGTGTCTAGGCAGGAAGTAAGAGATGCTGCTCGTGCCTATATAGGCGACACAGGCTTGCTAGACTTCGTGTTAAAGTCGTTGGGCAACCAAATTGTGGGGAATTACTTGGTTCGTCGCAGCTTGAATCCGGTGACCAAAGTTCTCGAATATTGCTTAGATGACCTTTCTAATGTCTCCCCTAACCAAGATGGCAATGTCATGACCAACTCCAAAGTGAAGGGATGGTACAAGATTTCGAGGTTCCAGCTAATGAAGGACATGTTCTACTTGTACAAGTACATCCTCAAAGACCAGAATCTAAGCACTAGTGCTACCACAGGAATCTTCTCAGCTATTCCCATGGCAGTTAGGATAATTCTTCATTCCAAGTACCTTGTCAAGGATTACAGCGGCGGGTTACCGCAAGTGAAACTCGAATTAGGACCTGAAGGAAAATCAAACATCTATTGTACCGTAATGATACGAAACAGTACACAAGTGTTTGATCATAACAAGATGATTAGCAGCAATGAAATAGCATTGCCTCCATACGAGTGTATAACACTGAAAAACAATGCTACAATCAATGATCTTAAGCTCGAAGTGGAGAGGAACTTCAAAGAGATCTACTGGGGATTGAAAAGCTTCGTCGTCGAAACGATTGCGAATTCCATTATGGATAATGCAAATGGAACAGATACGGTTTTTGGGCTGGTTGAGGTGGGTGGAAAGATTGTGCTTGAAGGAAGCAACAAAGATATGAGAGGTGGTGGAGTTGGTGGAGTTGGACTTGATAGAGAGCTTGAAGGACCCATATCTGAATGCGGGTCGGGTAATTGCATTGTGGACTGTCCGTGTGGGGCCAAAGATGACGACGGGGAAAGAATGGTGTGCTGTGACATTTGTGAAGTGTGGCAGCATACTCTGTGCGTTCGAATTCCAAACAATGAACAAGTTCCTCATATATTTCTCTGCAATCGATGCGAGAAAGAAATCATACTCTTACCTTCTCCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007275 GO:0008150 GO:0009555 GO:0009653 GO:0009719 GO:0009725 GO:0009741 GO:0009791 GO:0009846 GO:0009856 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009987 GO:0010033 GO:0010208 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010927 GO:0010941 GO:0010942 GO:0014070 GO:0016043 GO:0019219 GO:0019222 GO:0022414 GO:0022607 GO:0030198 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0032870 GO:0032989 GO:0033993 GO:0042221 GO:0043062 GO:0043067 GO:0043068 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044085 GO:0044424 GO:0044464 GO:0044706 GO:0045229 GO:0045893 GO:0045935 GO:0048229 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048444 GO:0048448 GO:0048466 GO:0048518 GO:0048522 GO:0048608 GO:0048646 GO:0048653 GO:0048654 GO:0048655 GO:0048658 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051704 GO:0051716 GO:0055046 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0071310 GO:0071367 GO:0071396 GO:0071407 GO:0071495 GO:0071840 GO:0080090 GO:0085029 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0099402 GO:1901700 GO:1901701 GO:1902680 GO:1903506 GO:1903508 GO:1905392 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PHD

Protein Analysis

726

Amino Acids

80.95

Weight (kDa)

7.72

Isoelectric Point (pI)

35.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WHD_plant_repro	PF25874	370 - 448	1.3e-33	Winged helix-turn-helix domain in plant reproductive proteins
Ubiquitin_At1g33420	PF25565	538 - 637	3.4e-27	At1g33420-like, ubiquitin-like domain
PHD	PF00628	671 - 715	3.3e-07	PHD-finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006001)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G22260
fragaria_vesca	FvH4_4g35820 FvH4_4g35830 FvH4_4g35830
malus_domestica	MD16G1012700.v1.1
prunus_persica	Prupe.1G338600_v2.0.a1
pyrus_communis	pycom16g01190
rosa_chinensis	RchiOBHm_Chr4g0445351 RchiOBHm_Chr4g0445361
rosa_laevigata	RLG00000005745 RLG00000005746
rosa_multiflora	Rmu_sc0000050.1_g000007 Rmu_sc0000050.1_g000008 Rmu_sc0000050.1_g000009
rosa_roxburghii	Rroxscaffold_5G00385870 Rroxscaffold_5G00385880
rosa_rugosa	Rorug04G0361000
rosa_samantha	Rh4AG422000 Rh4AG422100 Rh4BG434000 Rh4BG434100 Rh4CG448900 Rh4CG449000 Rh4DG430000 Rh4DG430100
rosa_wichuraiana	Rw4G036200 Rw4G036210

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	810
Acc65I	GGTACC	1 cut(s)	171
AccB1I	GGYRCC	1 cut(s)	171
AccB7I	CCANNNNNTGG	1 cut(s)	1276
AccBSI	CCGCTC	1 cut(s)	635
AciI	CCGC	7 cut(s)	464, 530, 635, 1572, 1575, 1583, 1986
AclWI	GGATC	2 cut(s)	690, 987
AcoI	YGGCCR	1 cut(s)	817
AcsI	RAATTY	4 cut(s)	553, 812, 1828, 2097
AcuI	CTGAAG	1 cut(s)	1629
AcyI	GRCGYC	1 cut(s)	1136
AdeI	CACNNNGTG	1 cut(s)	436
AfeI	AGCGCT	1 cut(s)	219
AfiI	CCNNNNNNNGG	4 cut(s)	794, 1164, 1276, 1561
AflII	CTTAAG	1 cut(s)	1754
AflIII	ACRYGT	1 cut(s)	1440
AhlI	ACTAGT	1 cut(s)	1487
AjnI	CCWGG	2 cut(s)	655, 788
AleI	CACNNNNGTG	1 cut(s)	458
Alw21I	GWGCWC	2 cut(s)	695, 1029
Alw26I	GTCTC	4 cut(s)	1091, 1119, 1143, 1360
Alw44I	GTGCAC	1 cut(s)	691
AlwI	GGATC	2 cut(s)	690, 987
Ama87I	CYCGRG	2 cut(s)	1081, 1140
Aor51HI	AGCGCT	1 cut(s)	219
AoxI	GGCC	4 cut(s)	354, 817, 934, 2022
ApaLI	GTGCAC	1 cut(s)	691
ApeKI	GCWGC	5 cut(s)	467, 1208, 1299, 1686, 2077
ApoI	RAATTY	4 cut(s)	553, 812, 1828, 2097
Asp700I	GAANNNNTTC	2 cut(s)	1805, 2115
Asp718I	GGTACC	1 cut(s)	171
AspLEI	GCGC	2 cut(s)	220, 860
AspS9I	GGNCC	4 cut(s)	100, 1604, 1970, 2022
AsuC2I	CCSGG	4 cut(s)	176, 1036, 1082, 1083
AsuHPI	GGTGA	3 cut(s)	1003, 1013, 1324
AsuII	TTCGAA	1 cut(s)	2095
AvaI	CYCGRG	2 cut(s)	1081, 1140
AvaII	GGWCC	3 cut(s)	100, 1604, 1970
BaeGI	GKGCMC	1 cut(s)	695
BaeI	ACNNNNGTAYC	4 cut(s)	659, 692, 833, 866
BanI	GGYRCC	1 cut(s)	171
BanII	GRGCYC	3 cut(s)	789, 1029, 1171
BarI	GAAGNNNNNNTAC	2 cut(s)	1391, 1423
BauI	CACGAG	2 cut(s)	1060, 1212
Bbv12I	GWGCWC	2 cut(s)	695, 1029
BbvI	GCAGC	5 cut(s)	479, 1195, 1311, 1698, 2089
BccI	CCATC	6 cut(s)	451, 912, 1104, 1173, 1364, 1398
BceAI	ACGGC	2 cut(s)	619, 804
BcgI	CGANNNNNNTGC	2 cut(s)	1805, 1839
BciT130I	CCWGG	2 cut(s)	657, 790
BclI	TGATCA	1 cut(s)	1666
BcnI	CCSGG	4 cut(s)	176, 1036, 1082, 1083
BcoDI	GTCTC	4 cut(s)	1091, 1119, 1143, 1360
BcuI	ACTAGT	1 cut(s)	1487
BfaI	CTAG	4 cut(s)	1022, 1188, 1241, 1488
BfmI	CTRYAG	1 cut(s)	13
BfoI	RGCGCY	2 cut(s)	221, 861
BfrI	CTTAAG	1 cut(s)	1754
BglII	AGATCT	1 cut(s)	1785
BisI	GCNGC	7 cut(s)	465, 468, 1209, 1300, 1573, 1687, 2078
BlsI	GCNGC	7 cut(s)	466, 469, 1210, 1301, 1574, 1688, 2079
Bme1390I	CCNGG	6 cut(s)	176, 657, 790, 1036, 1082, 1083
Bme18I	GGWCC	3 cut(s)	100, 1604, 1970
BmeT110I	CYCGRG	2 cut(s)	1081, 1140
BmgT120I	GGNCC	4 cut(s)	100, 1604, 1970, 2022
BmiI	GGNNCC	4 cut(s)	173, 1424, 1972, 2023
BmrFI	CCNGG	6 cut(s)	176, 657, 790, 1036, 1082, 1083
BmrI	ACTGGG	1 cut(s)	1801
BmsI	GCATC	4 cut(s)	244, 328, 1195, 2132
BmuI	ACTGGG	1 cut(s)	1801
BpmI	CTGGAG	2 cut(s)	260, 278
Bpu14I	TTCGAA	1 cut(s)	2095
BpuEI	CTTGAG	3 cut(s)	692, 943, 1013
BpuMI	CCSGG	4 cut(s)	176, 1036, 1082, 1083
Bsa29I	ATCGAT	2 cut(s)	685, 2140
BsaAI	YACGTR	2 cut(s)	391, 704
BsaHI	GRCGYC	1 cut(s)	1136
BsaI	GGTCTC	1 cut(s)	1119
BsaJI	CCNNGG	5 cut(s)	766, 788, 820, 1081, 1521
BsaWI	WCCGGW	1 cut(s)	1309
Bsc4I	CCNNNNNNNGG	4 cut(s)	794, 1164, 1276, 1561
Bse1I	ACTGG	2 cut(s)	496, 1796
Bse3DI	GCAATG	5 cut(s)	313, 370, 1381, 1696, 1700
BseBI	CCWGG	2 cut(s)	657, 790
BseCI	ATCGAT	2 cut(s)	685, 2140
BseDI	CCNNGG	5 cut(s)	766, 788, 820, 1081, 1521
BseGI	GGATG	5 cut(s)	276, 762, 897, 1409, 1461
BseLI	CCNNNNNNNGG	4 cut(s)	794, 1164, 1276, 1561
BseMI	GCAATG	5 cut(s)	313, 370, 1381, 1696, 1700
BseMII	CTCAG	2 cut(s)	95, 1524
BseNI	ACTGG	2 cut(s)	496, 1796
BseSI	GKGCMC	1 cut(s)	695
BseXI	GCAGC	5 cut(s)	479, 1195, 1311, 1698, 2089
BseYI	CCCAGC	1 cut(s)	396
BshFI	GGCC	4 cut(s)	356, 819, 936, 2024
BshNI	GGYRCC	1 cut(s)	171
BshVI	ATCGAT	2 cut(s)	685, 2140
BsiHKAI	GWGCWC	2 cut(s)	695, 1029
BsiHKCI	CYCGRG	2 cut(s)	1081, 1140
BsiSI	CCGG	5 cut(s)	176, 527, 1036, 1082, 1310
BslFI	GGGAC	1 cut(s)	1018
BslI	CCNNNNNNNGG	4 cut(s)	794, 1164, 1276, 1561
BsmAI	GTCTC	4 cut(s)	1091, 1119, 1143, 1360
BsmBI	CGTCTC	1 cut(s)	1143
BsmFI	GGGAC	1 cut(s)	1018
BsmI	GAATGC	3 cut(s)	879, 1092, 1988
BsnI	GGCC	4 cut(s)	356, 819, 936, 2024
Bso31I	GGTCTC	1 cut(s)	1119
BsoBI	CYCGRG	2 cut(s)	1081, 1140
Bsp119I	TTCGAA	1 cut(s)	2095
Bsp1286I	GDGCHC	4 cut(s)	695, 789, 1029, 1171
Bsp1407I	TGTACA	1 cut(s)	1452
Bsp143I	GATC	8 cut(s)	6, 682, 979, 1003, 1051, 1666, 1750, 1785
Bsp19I	CCATGG	1 cut(s)	1521
BspACI	CCGC	7 cut(s)	464, 530, 635, 1572, 1575, 1583, 1986
BspANI	GGCC	4 cut(s)	356, 819, 936, 2024
BspCNI	CTCAG	2 cut(s)	96, 1523
BspDI	ATCGAT	2 cut(s)	685, 2140
BspHI	TCATGA	1 cut(s)	1380
BspLI	GGNNCC	4 cut(s)	173, 1424, 1972, 2023
BspPI	GGATC	2 cut(s)	690, 987
BspQI	GCTCTTC	1 cut(s)	269
BspT104I	TTCGAA	1 cut(s)	2095
BspT107I	GGYRCC	1 cut(s)	171
BspTI	CTTAAG	1 cut(s)	1754
BspTNI	GGTCTC	1 cut(s)	1119
BsrBI	CCGCTC	1 cut(s)	635
BsrDI	GCAATG	5 cut(s)	313, 370, 1381, 1696, 1700
BsrGI	TGTACA	1 cut(s)	1452
BsrI	ACTGG	2 cut(s)	496, 1796
BssECI	CCNNGG	5 cut(s)	766, 788, 820, 1081, 1521
BssMI	GATC	8 cut(s)	6, 682, 979, 1003, 1051, 1666, 1750, 1785
BssNI	GRCGYC	1 cut(s)	1136
BssSI	CACGAG	2 cut(s)	1060, 1212
BssT1I	CCWWGG	1 cut(s)	1521
Bst2BI	CACGAG	2 cut(s)	1060, 1212
Bst2UI	CCWGG	2 cut(s)	657, 790
Bst4CI	ACNGT	6 cut(s)	368, 782, 1636, 1652, 1864, 2012
Bst6I	CTCTTC	6 cut(s)	113, 119, 199, 269, 335, 734
BstACI	GRCGYC	1 cut(s)	1136
BstAFI	CTTAAG	1 cut(s)	1754
BstAPI	GCANNNNNTGC	4 cut(s)	381, 580, 921, 2142
BstAUI	TGTACA	1 cut(s)	1452
BstBAI	YACGTR	2 cut(s)	391, 704
BstBI	TTCGAA	1 cut(s)	2095
BstC8I	GCNNGC	3 cut(s)	377, 913, 1238
BstDEI	CTNAG	4 cut(s)	104, 1336, 1481, 1510
BstDSI	CCRYGG	3 cut(s)	766, 820, 1521
BstEII	GGTNACC	2 cut(s)	1312, 1578
BstENI	CCTNNNNNAGG	2 cut(s)	1162, 1559
BstF5I	GGATG	5 cut(s)	276, 762, 897, 1409, 1461
BstH2I	RGCGCY	2 cut(s)	221, 861
BstHHI	GCGC	2 cut(s)	220, 860
BstKTI	GATC	8 cut(s)	9, 685, 982, 1006, 1054, 1669, 1753, 1788
BstMAI	GTCTC	4 cut(s)	1091, 1119, 1143, 1360
BstMBI	GATC	8 cut(s)	6, 682, 979, 1003, 1051, 1666, 1750, 1785
BstMWI	GCNNNNNNNGC	7 cut(s)	381, 580, 866, 921, 1214, 1233, 2142
BstNI	CCWGG	2 cut(s)	657, 790
BstNSI	RCATGY	2 cut(s)	583, 1444
BstPI	GGTNACC	2 cut(s)	1312, 1578
BstSCI	CCNGG	6 cut(s)	174, 655, 788, 1034, 1080, 1081
BstSFI	CTRYAG	1 cut(s)	13
BstSLI	GKGCMC	1 cut(s)	695
BstSNI	TACGTA	1 cut(s)	391
BstV1I	GCAGC	5 cut(s)	479, 1195, 1311, 1698, 2089
BstX2I	RGATCY	1 cut(s)	1785
BstXI	CCANNNNNNTGG	1 cut(s)	911
BstYI	RGATCY	1 cut(s)	1785
Bsu15I	ATCGAT	2 cut(s)	685, 2140
BsuRI	GGCC	4 cut(s)	356, 819, 936, 2024
BsuTUI	ATCGAT	2 cut(s)	685, 2140
BtgI	CCRYGG	3 cut(s)	766, 820, 1521
BtgZI	GCGATG	1 cut(s)	287
BtsCI	GGATG	5 cut(s)	276, 762, 897, 1409, 1461
BtsI	GCAGTG	1 cut(s)	379
BtsIMutI	CAGTG	3 cut(s)	379, 787, 1724
Cac8I	GCNNGC	3 cut(s)	377, 913, 1238
CciI	TCATGA	1 cut(s)	1380
CfoI	GCGC	2 cut(s)	220, 860
Cfr13I	GGNCC	4 cut(s)	100, 1604, 1970, 2022
Cfr9I	CCCGGG	1 cut(s)	1081
ClaI	ATCGAT	2 cut(s)	685, 2140
CseI	GACGC	1 cut(s)	1125
CspCI	CAANNNNNGTGG	2 cut(s)	475, 510
CviAII	CATG	8 cut(s)	580, 584, 748, 938, 1018, 1381, 1441, 1522
DdeI	CTNAG	4 cut(s)	104, 1336, 1481, 1510
DpnI	GATC	8 cut(s)	8, 684, 981, 1005, 1053, 1668, 1752, 1787
DpnII	GATC	8 cut(s)	6, 682, 979, 1003, 1051, 1666, 1750, 1785
DraI	TTTAAA	1 cut(s)	723
DraIII	CACNNNGTG	1 cut(s)	436
EaeI	YGGCCR	1 cut(s)	817
Eam1104I	CTCTTC	6 cut(s)	113, 119, 199, 269, 335, 734
EarI	CTCTTC	6 cut(s)	113, 119, 199, 269, 335, 734
Ecl136II	GAGCTC	1 cut(s)	1027
Eco105I	TACGTA	1 cut(s)	391
Eco130I	CCWWGG	1 cut(s)	1521
Eco147I	AGGCCT	1 cut(s)	356
Eco24I	GRGCYC	3 cut(s)	789, 1029, 1171
Eco31I	GGTCTC	1 cut(s)	1119
Eco47I	GGWCC	3 cut(s)	100, 1604, 1970
Eco47III	AGCGCT	1 cut(s)	219
Eco53kI	GAGCTC	1 cut(s)	1027
Eco57I	CTGAAG	1 cut(s)	1629
Eco88I	CYCGRG	2 cut(s)	1081, 1140
Eco91I	GGTNACC	2 cut(s)	1312, 1578
EcoICRI	GAGCTC	1 cut(s)	1027
EcoNI	CCTNNNNNAGG	2 cut(s)	1162, 1559
EcoO109I	RGGNCCY	2 cut(s)	1604, 1970
EcoO65I	GGTNACC	2 cut(s)	1312, 1578
EcoRI	GAATTC	2 cut(s)	1828, 2097
EcoRII	CCWGG	2 cut(s)	655, 788
EcoT14I	CCWWGG	1 cut(s)	1521
EcoT22I	ATGCAT	1 cut(s)	585
EcoT38I	GRGCYC	3 cut(s)	789, 1029, 1171
ErhI	CCWWGG	1 cut(s)	1521
Esp3I	CGTCTC	1 cut(s)	1143
FaeI	CATG	8 cut(s)	583, 587, 751, 941, 1021, 1384, 1444, 1525
FalI	AAGNNNNNCTT	2 cut(s)	416, 448
FaqI	GGGAC	1 cut(s)	1018
FatI	CATG	8 cut(s)	579, 583, 747, 937, 1017, 1380, 1440, 1521
FauI	CCCGC	3 cut(s)	523, 1568, 1979
FauNDI	CATATG	1 cut(s)	408
FbaI	TGATCA	1 cut(s)	1666
Fnu4HI	GCNGC	7 cut(s)	465, 468, 1209, 1300, 1573, 1687, 2078
FokI	GGATG	5 cut(s)	283, 749, 884, 1416, 1448
FriOI	GRGCYC	3 cut(s)	789, 1029, 1171
Fsp4HI	GCNGC	7 cut(s)	465, 468, 1209, 1300, 1573, 1687, 2078
FspBI	CTAG	4 cut(s)	1022, 1188, 1241, 1488
GlaI	GCGC	2 cut(s)	219, 859
GluI	GCNGC	7 cut(s)	465, 468, 1209, 1300, 1573, 1687, 2078
GsaI	CCCAGC	1 cut(s)	400
GsuI	CTGGAG	2 cut(s)	260, 278
HaeII	RGCGCY	2 cut(s)	221, 861
HaeIII	GGCC	4 cut(s)	356, 819, 936, 2024
HapII	CCGG	5 cut(s)	176, 527, 1036, 1082, 1310
HgaI	GACGC	1 cut(s)	1125
HhaI	GCGC	2 cut(s)	220, 860
Hin1I	GRCGYC	1 cut(s)	1136
Hin1II	CATG	8 cut(s)	583, 587, 751, 941, 1021, 1384, 1444, 1525
Hin6I	GCGC	2 cut(s)	218, 858
HinP1I	GCGC	2 cut(s)	218, 858
HincII	GTYRAC	1 cut(s)	481
HindII	GTYRAC	1 cut(s)	481
HindIII	AAGCTT	1 cut(s)	1804
HinfI	GANTC	5 cut(s)	994, 1070, 1306, 1477, 1503
HpaII	CCGG	5 cut(s)	176, 527, 1036, 1082, 1310
HphI	GGTGA	3 cut(s)	1003, 1013, 1324
Hpy166II	GTNNAC	4 cut(s)	481, 693, 1655, 2008
Hpy188I	TCNGA	4 cut(s)	649, 979, 1178, 1981
Hpy188III	TCNNGA	7 cut(s)	98, 151, 224, 239, 1140, 1325, 1381
Hpy8I	GTNNAC	4 cut(s)	481, 693, 1655, 2008
HpyAV	CCTTC	7 cut(s)	453, 1393, 1429, 1604, 1898, 1961, 2178
HpyCH4III	ACNGT	6 cut(s)	368, 782, 1636, 1652, 1864, 2012
HpyCH4IV	ACGT	6 cut(s)	155, 210, 390, 516, 703, 729
HpyF10VI	GCNNNNNNNGC	7 cut(s)	381, 580, 866, 921, 1214, 1233, 2142
HpyF3I	CTNAG	4 cut(s)	104, 1336, 1481, 1510
HpySE526I	ACGT	6 cut(s)	155, 210, 390, 516, 703, 729
Hsp92I	GRCGYC	1 cut(s)	1136
Hsp92II	CATG	8 cut(s)	583, 587, 751, 941, 1021, 1384, 1444, 1525
HspAI	GCGC	2 cut(s)	218, 858
KpnI	GGTACC	1 cut(s)	175
Ksp22I	TGATCA	1 cut(s)	1666
Kzo9I	GATC	8 cut(s)	6, 682, 979, 1003, 1051, 1666, 1750, 1785
LguI	GCTCTTC	1 cut(s)	269
LmnI	GCTCC	4 cut(s)	111, 297, 316, 632
Lsp1109I	GCAGC	5 cut(s)	479, 1195, 1311, 1698, 2089
LweI	GCATC	4 cut(s)	244, 328, 1195, 2132
MaeI	CTAG	4 cut(s)	1022, 1188, 1241, 1488
MaeII	ACGT	6 cut(s)	155, 210, 390, 516, 703, 729
MaeIII	GTNAC	5 cut(s)	835, 991, 1312, 1578, 2057
MalI	GATC	8 cut(s)	8, 684, 981, 1005, 1053, 1668, 1752, 1787
MbiI	CCGCTC	1 cut(s)	635
MboI	GATC	8 cut(s)	6, 682, 979, 1003, 1051, 1666, 1750, 1785
MflI	RGATCY	1 cut(s)	1785
MhlI	GDGCHC	4 cut(s)	695, 789, 1029, 1171
MlyI	GAGTC	1 cut(s)	988
MmeI	TCCRAC	2 cut(s)	957, 1927
Mph1103I	ATGCAT	1 cut(s)	585
MroXI	GAANNNNTTC	2 cut(s)	1805, 2115
MseI	TTAA	4 cut(s)	722, 1160, 1253, 1755
MslI	CAYNNNNRTG	3 cut(s)	306, 458, 1714
MspA1I	CMGCKG	1 cut(s)	1572
MspCI	CTTAAG	1 cut(s)	1754
MspI	CCGG	5 cut(s)	176, 527, 1036, 1082, 1310
MspR9I	CCNGG	6 cut(s)	176, 657, 790, 1036, 1082, 1083
MssI	GTTTAAAC	1 cut(s)	723
Mva1269I	GAATGC	3 cut(s)	879, 1092, 1988
MvaI	CCWGG	2 cut(s)	657, 790
MwoI	GCNNNNNNNGC	7 cut(s)	381, 580, 866, 921, 1214, 1233, 2142
NciI	CCSGG	4 cut(s)	176, 1036, 1082, 1083
NcoI	CCATGG	1 cut(s)	1521
NdeI	CATATG	1 cut(s)	408
NdeII	GATC	8 cut(s)	6, 682, 979, 1003, 1051, 1666, 1750, 1785
NlaIII	CATG	8 cut(s)	583, 587, 751, 941, 1021, 1384, 1444, 1525
NlaIV	GGNNCC	4 cut(s)	173, 1424, 1972, 2023
NmuCI	GTSAC	4 cut(s)	835, 991, 1312, 2057
NsiI	ATGCAT	1 cut(s)	585
NspI	RCATGY	2 cut(s)	583, 1444
NspV	TTCGAA	1 cut(s)	2095
OliI	CACNNNNGTG	1 cut(s)	458
PaeR7I	CTCGAG	1 cut(s)	1140
PagI	TCATGA	1 cut(s)	1380
PceI	AGGCCT	1 cut(s)	356
PciI	ACATGT	1 cut(s)	1440
PciSI	GCTCTTC	1 cut(s)	269
PcsI	WCGNNNNNNNCGW	1 cut(s)	330
PctI	GAATGC	3 cut(s)	879, 1092, 1988
PdmI	GAANNNNTTC	2 cut(s)	1805, 2115
PfeI	GAWTC	4 cut(s)	1070, 1306, 1477, 1503
PflMI	CCANNNNNTGG	1 cut(s)	1276
PkrI	GCNGC	7 cut(s)	466, 469, 1210, 1301, 1574, 1688, 2079
PleI	GAGTC	1 cut(s)	988
PmeI	GTTTAAAC	1 cut(s)	723
PpsI	GAGTC	1 cut(s)	988
Ppu21I	YACGTR	2 cut(s)	391, 704
PpuMI	RGGWCCY	2 cut(s)	1604, 1970
PscI	ACATGT	1 cut(s)	1440
PsiI	TTATAA	1 cut(s)	810
Psp124BI	GAGCTC	1 cut(s)	1029
Psp5II	RGGWCCY	2 cut(s)	1604, 1970
Psp6I	CCWGG	2 cut(s)	655, 788
PspEI	GGTNACC	2 cut(s)	1312, 1578
PspFI	CCCAGC	1 cut(s)	396
PspGI	CCWGG	2 cut(s)	655, 788
PspN4I	GGNNCC	4 cut(s)	173, 1424, 1972, 2023
PspPI	GGNCC	4 cut(s)	100, 1604, 1970, 2022
PspPPI	RGGWCCY	2 cut(s)	1604, 1970
PsuI	RGATCY	1 cut(s)	1785
RseI	CAYNNNNRTG	3 cut(s)	306, 458, 1714
SacI	GAGCTC	1 cut(s)	1029
SapI	GCTCTTC	1 cut(s)	269
SaqAI	TTAA	4 cut(s)	722, 1160, 1253, 1755
SatI	GCNGC	7 cut(s)	465, 468, 1209, 1300, 1573, 1687, 2078
Sau3AI	GATC	8 cut(s)	6, 682, 979, 1003, 1051, 1666, 1750, 1785
Sau96I	GGNCC	4 cut(s)	100, 1604, 1970, 2022
SchI	GAGTC	1 cut(s)	988
ScrFI	CCNGG	6 cut(s)	176, 657, 790, 1036, 1082, 1083
SduI	GDGCHC	4 cut(s)	695, 789, 1029, 1171
SfaNI	GCATC	4 cut(s)	244, 328, 1195, 2132
SfcI	CTRYAG	1 cut(s)	13
Sfr274I	CTCGAG	1 cut(s)	1140
SfuI	TTCGAA	1 cut(s)	2095
SinI	GGWCC	3 cut(s)	100, 1604, 1970
SlaI	CTCGAG	1 cut(s)	1140
SmaI	CCCGGG	1 cut(s)	1083
SmiMI	CAYNNNNRTG	3 cut(s)	306, 458, 1714
SmlI	CTYRAG	5 cut(s)	671, 958, 1028, 1140, 1754
SmoI	CTYRAG	5 cut(s)	671, 958, 1028, 1140, 1754
SnaBI	TACGTA	1 cut(s)	391
SpeI	ACTAGT	1 cut(s)	1487
SseBI	AGGCCT	1 cut(s)	356
SsiI	CCGC	7 cut(s)	464, 530, 635, 1572, 1575, 1583, 1986
SspI	AATATT	1 cut(s)	1331
SspMI	CTAG	4 cut(s)	1022, 1188, 1241, 1488
SstI	GAGCTC	1 cut(s)	1029
StuI	AGGCCT	1 cut(s)	356
StyD4I	CCNGG	6 cut(s)	174, 655, 788, 1034, 1080, 1081
StyI	CCWWGG	1 cut(s)	1521
TaaI	ACNGT	6 cut(s)	368, 782, 1636, 1652, 1864, 2012
TaiI	ACGT	6 cut(s)	158, 213, 393, 519, 706, 732
TatI	WGTACW	3 cut(s)	1452, 1458, 1652
TauI	GCSGC	2 cut(s)	467, 1575
TfiI	GAWTC	4 cut(s)	1070, 1306, 1477, 1503
Tru1I	TTAA	4 cut(s)	722, 1160, 1253, 1755
Tru9I	TTAA	4 cut(s)	722, 1160, 1253, 1755
TscAI	CASTG	3 cut(s)	386, 787, 1731
TseFI	GTSAC	4 cut(s)	835, 991, 1312, 2057
TseI	GCWGC	5 cut(s)	467, 1208, 1299, 1686, 2077
Tsp45I	GTSAC	4 cut(s)	835, 991, 1312, 2057
TspDTI	ATGAA	8 cut(s)	287, 290, 975, 1006, 1448, 1532, 1707, 2124
TspGWI	ACGGA	4 cut(s)	464, 783, 1061, 2004
TspMI	CCCGGG	1 cut(s)	1081
TspRI	CASTG	3 cut(s)	386, 787, 1731
Van91I	CCANNNNNTGG	1 cut(s)	1276
Vha464I	CTTAAG	1 cut(s)	1754
VneI	GTGCAC	1 cut(s)	691
VpaK11BI	GGWCC	3 cut(s)	100, 1604, 1970
XagI	CCTNNNNNAGG	2 cut(s)	1162, 1559
XapI	RAATTY	4 cut(s)	553, 812, 1828, 2097
XceI	RCATGY	2 cut(s)	583, 1444
XcmI	CCANNNNNNNNNTGG	1 cut(s)	908
XhoI	CTCGAG	1 cut(s)	1140
XmaI	CCCGGG	1 cut(s)	1081
XmnI	GAANNNNTTC	2 cut(s)	1805, 2115
XspI	CTAG	4 cut(s)	1022, 1188, 1241, 1488
Zsp2I	ATGCAT	1 cut(s)	585

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.