Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000005

Orthogroup ID

OG0006001

Physical Location & Seq

Genomic Coordinates Forward (+)

64861756 .. 64864650

Gene Structure 2895 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_5G00385870.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1866 bp

ATGTCGTCACATAATCTGGACCTGACTGGAAGCAAGAAGAGAAAGAGAAGCGAGAGGGTTTTCAGGTTCAAGACTTTTGGAGAAAACGGTTATCCAGTCGGGTTCGTTGCCTCGTCGTTTAGGGAAAACGTGAAAGCGCTTCTCGAATATGGGCATCTGGAGAGTACCCTATGCAATAGTGCTACTGGAATAAAGAGCTGGTCGTTCCATCTCCAGCTTCATCGTAATGCTCGTGTGCATCTCGTTCTCTTCGTCGTCGAAGAACCTATTGAGGCCTCACTGAACCGTCATTGCAAGCAGTGCCAATACGTCGGCTGGGGGCAGCATATGGTTTGCAATAAGAAGTATCACTTTGTGTTGCCTTCGACGGACACGATGGTGGCGGCTGCTTGTTTGATCAACTGCAAAGTTGCCGGAGGCAATAACAACGACGATGATATTCCGGCGGGTAAGTCATCGTCGTTGAATTTACTGGAATTAGAGGGGCATATCATGCATGGTGTATTTCACTCTAACGGGTTCGGGCATTTGCTTTGTGTCAATGGCGCGGAAACGGGTTCGGGCTTGCCTGGACACCAGATACTAGAGTTTTGGGATCGATTGTGCTCGGGATTACGAGCAAGGAAAGTGAGTTTAAACGACGTTTCACAGAAAAATGGCATGGAGTTGAGGCTCATCCATGGAGTAGCATACAGTGAGCCCTGGTTTGGTCGTTGGGGTTACAAATTTGGCCGTGGAACATTTTGTGTCACTCAACAAATGTACCAAAGCGCCATTGAAGCATTACAAGGCATGCCCTTGTGCTTACTCATCCACCATCTTGCTGACATTACTTGCAACCAATTAGGCCACGATATTTCCCTCATCTTCTCAAGATGGTCTCCTAAAAGAGTTGAAATGGCGTCTCGGGTGATTGTGGAAGCCTTAAAGAGGGCTCATTTCAGATGGGTCTCTCGGCAGGAAGTAAGGGATGCTGCTCGCTCGTATATAGGGGACACGGGCTTGCTAGACTTCGTGTTGAAGTCGTTGGGCAACCAAATTGTCGGGAATTACTTGGTTCGTCGCAGCTTGAATCCGGTGACCAAAGTTCTCGAGTATTGCTTAGAAGACCTTTCTAATGTGTCCCCTAAACAAGATGGCAATGTCATGGCCAACTCCAAAGTGAAGGGATGGTACAAGATTTCGAGGTTCCAGCTAATGAAGGACATGTTCTACTTGTACAGGTACATCCTCAAAGACCAAAAGCTAAGCACCGGTACTACTACAGGGATCTTTTCAGCAATTCCGATGGCTGTTAGGATCATTCTTGATTCCAAGTACCTTGTCAAAGATTACTGCGGCGAGTTACGGCATGTGAAACTAGAATTAGGACCTGAAGGAAAATCAAATCTCTATTGCACCGTAATGCTAAGAAACAGTACACAAGTGCTTGATTATAACAAGATGATTAACAACAAAGAATTATTATTGCCGCCATACGAGTGTGTGATACTGAAAAACAATGCTACCATCAATGATCTTAAGCTCGAAGTGGAGAGGAACTTCAAAGAGATCTACTGGGGATTGAAAAGCTTCGTTGTGGAAACGATTGCGAATTTGAGTATCGATGCAAAGGGAACAGATATGGTTTTTGGGCTGGTTGAGGTGGGTGGGAAGATTGTGCTTGAAGGAAGCGACAAAGATATGAGAGGTGGTGGACTTGGACTTGTTAGAGAGCATGAAGGACCCATATTAGAATGCGGGTCGGTTAACTGCATTGTGGACTGTCCATGTGGCGCCAAAGATGACGACGGGGAAAGAATGGTGTGCTGCGACATATGTGAAGTGTGGCAGCATACTCTCAACATGGTATCTAGTCATGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007275 GO:0008150 GO:0009555 GO:0009653 GO:0009719 GO:0009725 GO:0009741 GO:0009791 GO:0009846 GO:0009856 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009987 GO:0010033 GO:0010208 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010927 GO:0010941 GO:0010942 GO:0014070 GO:0016043 GO:0019219 GO:0019222 GO:0022414 GO:0022607 GO:0030198 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0032870 GO:0032989 GO:0033993 GO:0042221 GO:0043062 GO:0043067 GO:0043068 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044085 GO:0044424 GO:0044464 GO:0044706 GO:0045229 GO:0045893 GO:0045935 GO:0048229 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048444 GO:0048448 GO:0048466 GO:0048518 GO:0048522 GO:0048608 GO:0048646 GO:0048653 GO:0048654 GO:0048655 GO:0048658 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051704 GO:0051716 GO:0055046 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0071310 GO:0071367 GO:0071396 GO:0071407 GO:0071495 GO:0071840 GO:0080090 GO:0085029 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0099402 GO:1901700 GO:1901701 GO:1902680 GO:1903506 GO:1903508 GO:1905392 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PHD

Protein Analysis

621

Amino Acids

69.77

Weight (kDa)

8.42

Isoelectric Point (pI)

35.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WHD_plant_repro	PF25874	292 - 370	3.1e-34	Winged helix-turn-helix domain in plant reproductive proteins
Ubiquitin_At1g33420	PF25565	460 - 558	2.6e-28	At1g33420-like, ubiquitin-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006001)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G22260
fragaria_vesca	FvH4_4g35820 FvH4_4g35830 FvH4_4g35830
malus_domestica	MD16G1012700.v1.1
prunus_persica	Prupe.1G338600_v2.0.a1
pyrus_communis	pycom16g01190
rosa_chinensis	RchiOBHm_Chr4g0445351 RchiOBHm_Chr4g0445361
rosa_laevigata	RLG00000005745 RLG00000005746
rosa_multiflora	Rmu_sc0000050.1_g000007 Rmu_sc0000050.1_g000008 Rmu_sc0000050.1_g000009
rosa_roxburghii	Rroxscaffold_5G00385870 Rroxscaffold_5G00385880
rosa_rugosa	Rorug04G0361000
rosa_samantha	Rh4AG422000 Rh4AG422100 Rh4BG434000 Rh4BG434100 Rh4CG448900 Rh4CG449000 Rh4DG430000 Rh4DG430100
rosa_wichuraiana	Rw4G036200 Rw4G036210

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1437
AccB1I	GGYRCC	1 cut(s)	1775
AccII	CGCG	1 cut(s)	548
AciI	CCGC	6 cut(s)	383, 446, 548, 1338, 1472, 1740
AclWI	GGATC	3 cut(s)	603, 1277, 1307
AcoI	YGGCCR	2 cut(s)	730, 1149
AcsI	RAATTY	3 cut(s)	466, 725, 1594
AcuI	CTGAAG	1 cut(s)	1395
AcyI	GRCGYC	2 cut(s)	902, 1776
AdeI	CACNNNGTG	1 cut(s)	355
AfaI	GTAC	8 cut(s)	166, 764, 1175, 1220, 1226, 1258, 1319, 1420
AfeI	AGCGCT	1 cut(s)	138
AfiI	CCNNNNNNNGG	2 cut(s)	707, 930
AflII	CTTAAG	1 cut(s)	1520
AflIII	ACRYGT	1 cut(s)	1206
AgeI	ACCGGT	1 cut(s)	1253
AgsI	TTSAA	9 cut(s)	70, 466, 779, 896, 1021, 1072, 1546, 1567, 1667
AjnI	CCWGG	2 cut(s)	568, 701
AleI	CACNNNNGTG	1 cut(s)	377
AluBI	AGCT	7 cut(s)	198, 217, 1068, 1195, 1246, 1525, 1572
AluI	AGCT	7 cut(s)	198, 217, 1068, 1195, 1246, 1525, 1572
Alw21I	GWGCWC	1 cut(s)	608
Alw26I	GTCTC	3 cut(s)	885, 909, 955
AlwI	GGATC	3 cut(s)	603, 1277, 1307
Ama87I	CYCGRG	3 cut(s)	607, 906, 1091
Aor51HI	AGCGCT	1 cut(s)	138
AoxI	GGCC	4 cut(s)	273, 730, 847, 1149
ApeKI	GCWGC	6 cut(s)	322, 386, 974, 1065, 1809, 1831
ApoI	RAATTY	3 cut(s)	466, 725, 1594
AsiGI	ACCGGT	1 cut(s)	1253
Asp700I	GAANNNNTTC	1 cut(s)	1571
AspLEI	GCGC	4 cut(s)	139, 548, 773, 1778
AspS9I	GGNCC	3 cut(s)	19, 1370, 1724
AsuHPI	GGTGA	2 cut(s)	922, 1090
AvaI	CYCGRG	3 cut(s)	607, 906, 1091
AvaII	GGWCC	3 cut(s)	19, 1370, 1724
BaeI	ACNNNNGTAYC	4 cut(s)	572, 605, 746, 779
BalI	TGGCCA	1 cut(s)	1151
BanI	GGYRCC	1 cut(s)	1775
BanII	GRGCYC	2 cut(s)	702, 937
BarI	GAAGNNNNNNTAC	2 cut(s)	1157, 1189
BauI	CACGAG	1 cut(s)	231
BbsI	GAAGAC	1 cut(s)	1113
Bbv12I	GWGCWC	1 cut(s)	608
BbvI	GCAGC	6 cut(s)	334, 373, 961, 1077, 1796, 1843
BccI	CCATC	9 cut(s)	216, 370, 825, 870, 939, 1130, 1164, 1282, 1517
BceAI	ACGGC	2 cut(s)	717, 1364
BciT130I	CCWGG	2 cut(s)	570, 703
BclI	TGATCA	1 cut(s)	396
BcoDI	GTCTC	3 cut(s)	885, 909, 955
BfaI	CTAG	4 cut(s)	584, 1007, 1361, 1854
BfmI	CTRYAG	1 cut(s)	1263
BfoI	RGCGCY	3 cut(s)	140, 774, 1779
BfrI	CTTAAG	1 cut(s)	1520
BglII	AGATCT	1 cut(s)	1551
BisI	GCNGC	9 cut(s)	323, 384, 387, 975, 1066, 1339, 1472, 1810, 1832
BlpI	GCTNAGC	1 cut(s)	1247
BlsI	GCNGC	9 cut(s)	324, 385, 388, 976, 1067, 1340, 1473, 1811, 1833
Bme1390I	CCNGG	2 cut(s)	570, 703
Bme18I	GGWCC	3 cut(s)	19, 1370, 1724
BmeT110I	CYCGRG	3 cut(s)	607, 906, 1091
BmgT120I	GGNCC	3 cut(s)	19, 1370, 1724
BmiI	GGNNCC	3 cut(s)	1190, 1726, 1777
BmrFI	CCNGG	2 cut(s)	570, 703
BmrI	ACTGGG	1 cut(s)	1567
BmsI	GCATC	4 cut(s)	163, 247, 961, 1597
BmuI	ACTGGG	1 cut(s)	1567
BpiI	GAAGAC	1 cut(s)	1113
BpmI	CTGGAG	2 cut(s)	179, 197
Bpu1102I	GCTNAGC	1 cut(s)	1247
BpuEI	CTTGAG	1 cut(s)	856
Bsa29I	ATCGAT	2 cut(s)	598, 1605
BsaHI	GRCGYC	2 cut(s)	902, 1776
BsaI	GGTCTC	2 cut(s)	885, 955
BsaJI	CCNNGG	3 cut(s)	679, 701, 733
BsaWI	WCCGGW	2 cut(s)	1075, 1253
Bsc4I	CCNNNNNNNGG	2 cut(s)	707, 930
Bse118I	RCCGGY	1 cut(s)	1253
Bse1I	ACTGG	5 cut(s)	31, 95, 190, 477, 1562
Bse3DI	GCAATG	2 cut(s)	289, 1147
BseBI	CCWGG	2 cut(s)	570, 703
BseCI	ATCGAT	2 cut(s)	598, 1605
BseDI	CCNNGG	3 cut(s)	679, 701, 733
BseGI	GGATG	5 cut(s)	675, 810, 976, 1175, 1227
BseLI	CCNNNNNNNGG	2 cut(s)	707, 930
BseMI	GCAATG	2 cut(s)	289, 1147
BseNI	ACTGG	5 cut(s)	31, 95, 190, 477, 1562
BseXI	GCAGC	6 cut(s)	334, 373, 961, 1077, 1796, 1843
BseYI	CCCAGC	1 cut(s)	315
Bsh1236I	CGCG	1 cut(s)	548
BshFI	GGCC	4 cut(s)	275, 732, 849, 1151
BshNI	GGYRCC	1 cut(s)	1775
BshTI	ACCGGT	1 cut(s)	1253
BshVI	ATCGAT	2 cut(s)	598, 1605
BsiHKAI	GWGCWC	1 cut(s)	608
BsiHKCI	CYCGRG	3 cut(s)	607, 906, 1091
BsiSI	CCGG	4 cut(s)	414, 443, 1076, 1254
BslFI	GGGAC	2 cut(s)	1007, 1108
BslI	CCNNNNNNNGG	2 cut(s)	707, 930
BsmAI	GTCTC	3 cut(s)	885, 909, 955
BsmBI	CGTCTC	1 cut(s)	909
BsmFI	GGGAC	2 cut(s)	1007, 1108
BsmI	GAATGC	1 cut(s)	1742
BsnI	GGCC	4 cut(s)	275, 732, 849, 1151
Bso31I	GGTCTC	2 cut(s)	885, 955
BsoBI	CYCGRG	3 cut(s)	607, 906, 1091
Bsp1286I	GDGCHC	3 cut(s)	608, 702, 937
Bsp1407I	TGTACA	1 cut(s)	1218
Bsp143I	GATC	6 cut(s)	396, 595, 1269, 1299, 1516, 1551
Bsp1720I	GCTNAGC	1 cut(s)	1247
Bsp19I	CCATGG	1 cut(s)	679
BspACI	CCGC	6 cut(s)	383, 446, 548, 1338, 1472, 1740
BspANI	GGCC	4 cut(s)	275, 732, 849, 1151
BspDI	ATCGAT	2 cut(s)	598, 1605
BspFNI	CGCG	1 cut(s)	548
BspLI	GGNNCC	3 cut(s)	1190, 1726, 1777
BspPI	GGATC	3 cut(s)	603, 1277, 1307
BspT107I	GGYRCC	1 cut(s)	1775
BspTI	CTTAAG	1 cut(s)	1520
BspTNI	GGTCTC	2 cut(s)	885, 955
BsrDI	GCAATG	2 cut(s)	289, 1147
BsrFI	RCCGGY	1 cut(s)	1253
BsrGI	TGTACA	1 cut(s)	1218
BsrI	ACTGG	5 cut(s)	31, 95, 190, 477, 1562
BssAI	RCCGGY	1 cut(s)	1253
BssECI	CCNNGG	3 cut(s)	679, 701, 733
BssMI	GATC	6 cut(s)	396, 595, 1269, 1299, 1516, 1551
BssNI	GRCGYC	2 cut(s)	902, 1776
BssSI	CACGAG	1 cut(s)	231
BssT1I	CCWWGG	1 cut(s)	679
Bst2BI	CACGAG	1 cut(s)	231
Bst2UI	CCWGG	2 cut(s)	570, 703
Bst4CI	ACNGT	6 cut(s)	89, 287, 695, 1402, 1418, 1766
Bst6I	CTCTTC	2 cut(s)	32, 254
BstACI	GRCGYC	2 cut(s)	902, 1776
BstAFI	CTTAAG	1 cut(s)	1520
BstAPI	GCANNNNNTGC	2 cut(s)	300, 493
BstAUI	TGTACA	1 cut(s)	1218
BstC8I	GCNNGC	5 cut(s)	296, 566, 794, 979, 1004
BstDEI	CTNAG	3 cut(s)	1102, 1247, 1409
BstDSI	CCRYGG	2 cut(s)	679, 733
BstEII	GGTNACC	1 cut(s)	1078
BstENI	CCTNNNNNAGG	1 cut(s)	928
BstF5I	GGATG	5 cut(s)	675, 810, 976, 1175, 1227
BstFNI	CGCG	1 cut(s)	548
BstH2I	RGCGCY	3 cut(s)	140, 774, 1779
BstHHI	GCGC	4 cut(s)	139, 548, 773, 1778
BstKTI	GATC	6 cut(s)	399, 598, 1272, 1302, 1519, 1554
BstMAI	GTCTC	3 cut(s)	885, 909, 955
BstMBI	GATC	6 cut(s)	396, 595, 1269, 1299, 1516, 1551
BstMWI	GCNNNNNNNGC	3 cut(s)	300, 493, 779
BstNI	CCWGG	2 cut(s)	570, 703
BstNSI	RCATGY	3 cut(s)	796, 1210, 1355
BstPI	GGTNACC	1 cut(s)	1078
BstSCI	CCNGG	2 cut(s)	568, 701
BstSFI	CTRYAG	1 cut(s)	1263
BstUI	CGCG	1 cut(s)	548
BstV1I	GCAGC	6 cut(s)	334, 373, 961, 1077, 1796, 1843
BstV2I	GAAGAC	1 cut(s)	1113
BstX2I	RGATCY	2 cut(s)	1269, 1551
BstYI	RGATCY	2 cut(s)	1269, 1551
Bsu15I	ATCGAT	2 cut(s)	598, 1605
BsuRI	GGCC	4 cut(s)	275, 732, 849, 1151
BsuTUI	ATCGAT	2 cut(s)	598, 1605
BtgI	CCRYGG	2 cut(s)	679, 733
BtsCI	GGATG	5 cut(s)	675, 810, 976, 1175, 1227
BtsI	GCAGTG	1 cut(s)	305
BtsIMutI	CAGTG	3 cut(s)	278, 305, 700
Cac8I	GCNNGC	5 cut(s)	296, 566, 794, 979, 1004
CfoI	GCGC	4 cut(s)	139, 548, 773, 1778
Cfr10I	RCCGGY	1 cut(s)	1253
Cfr13I	GGNCC	3 cut(s)	19, 1370, 1724
ClaI	ATCGAT	2 cut(s)	598, 1605
CseI	GACGC	1 cut(s)	891
Csp6I	GTAC	8 cut(s)	165, 763, 1174, 1219, 1225, 1257, 1318, 1419
CspAI	ACCGGT	1 cut(s)	1253
CviQI	GTAC	8 cut(s)	165, 763, 1174, 1219, 1225, 1257, 1318, 1419
DdeI	CTNAG	3 cut(s)	1102, 1247, 1409
DinI	GGCGCC	1 cut(s)	1777
DpnI	GATC	6 cut(s)	398, 597, 1271, 1301, 1518, 1553
DpnII	GATC	6 cut(s)	396, 595, 1269, 1299, 1516, 1551
DraI	TTTAAA	1 cut(s)	636
DraIII	CACNNNGTG	1 cut(s)	355
EaeI	YGGCCR	2 cut(s)	730, 1149
Eam1104I	CTCTTC	2 cut(s)	32, 254
EarI	CTCTTC	2 cut(s)	32, 254
Eco130I	CCWWGG	1 cut(s)	679
Eco147I	AGGCCT	1 cut(s)	275
Eco24I	GRGCYC	2 cut(s)	702, 937
Eco31I	GGTCTC	2 cut(s)	885, 955
Eco47I	GGWCC	3 cut(s)	19, 1370, 1724
Eco47III	AGCGCT	1 cut(s)	138
Eco57I	CTGAAG	1 cut(s)	1395
Eco88I	CYCGRG	3 cut(s)	607, 906, 1091
Eco91I	GGTNACC	1 cut(s)	1078
EcoNI	CCTNNNNNAGG	1 cut(s)	928
EcoO109I	RGGNCCY	2 cut(s)	1370, 1724
EcoO65I	GGTNACC	1 cut(s)	1078
EcoRII	CCWGG	2 cut(s)	568, 701
EcoT14I	CCWWGG	1 cut(s)	679
EcoT22I	ATGCAT	1 cut(s)	498
EcoT38I	GRGCYC	2 cut(s)	702, 937
EgeI	GGCGCC	1 cut(s)	1777
EheI	GGCGCC	1 cut(s)	1777
ErhI	CCWWGG	1 cut(s)	679
Esp3I	CGTCTC	1 cut(s)	909
FalI	AAGNNNNNCTT	2 cut(s)	335, 367
FaqI	GGGAC	2 cut(s)	1007, 1108
FauI	CCCGC	2 cut(s)	439, 1733
FauNDI	CATATG	2 cut(s)	327, 1817
FbaI	TGATCA	1 cut(s)	396
Fnu4HI	GCNGC	9 cut(s)	323, 384, 387, 975, 1066, 1339, 1472, 1810, 1832
FokI	GGATG	5 cut(s)	662, 797, 983, 1182, 1214
FriOI	GRGCYC	2 cut(s)	702, 937
Fsp4HI	GCNGC	9 cut(s)	323, 384, 387, 975, 1066, 1339, 1472, 1810, 1832
FspBI	CTAG	4 cut(s)	584, 1007, 1361, 1854
GlaI	GCGC	4 cut(s)	138, 547, 772, 1777
GluI	GCNGC	9 cut(s)	323, 384, 387, 975, 1066, 1339, 1472, 1810, 1832
GsaI	CCCAGC	1 cut(s)	319
GsuI	CTGGAG	2 cut(s)	179, 197
HaeII	RGCGCY	3 cut(s)	140, 774, 1779
HaeIII	GGCC	4 cut(s)	275, 732, 849, 1151
HapII	CCGG	4 cut(s)	414, 443, 1076, 1254
HgaI	GACGC	1 cut(s)	891
HhaI	GCGC	4 cut(s)	139, 548, 773, 1778
Hin1I	GRCGYC	2 cut(s)	902, 1776
Hin6I	GCGC	4 cut(s)	137, 546, 771, 1776
HinP1I	GCGC	4 cut(s)	137, 546, 771, 1776
HincII	GTYRAC	1 cut(s)	1750
HindII	GTYRAC	1 cut(s)	1750
HindIII	AAGCTT	1 cut(s)	1570
HinfI	GANTC	2 cut(s)	1072, 1310
HpaI	GTTAAC	1 cut(s)	1750
HpaII	CCGG	4 cut(s)	414, 443, 1076, 1254
HphI	GGTGA	2 cut(s)	922, 1090
Hpy166II	GTNNAC	4 cut(s)	1421, 1697, 1750, 1762
Hpy188I	TCNGA	2 cut(s)	944, 1287
Hpy188III	TCNNGA	9 cut(s)	17, 70, 143, 158, 609, 873, 1045, 1091, 1307
Hpy8I	GTNNAC	4 cut(s)	1421, 1697, 1750, 1762
HpyAV	CCTTC	6 cut(s)	372, 1159, 1195, 1370, 1661, 1715
HpyCH4III	ACNGT	6 cut(s)	89, 287, 695, 1402, 1418, 1766
HpyCH4IV	ACGT	3 cut(s)	129, 309, 642
HpyF10VI	GCNNNNNNNGC	3 cut(s)	300, 493, 779
HpyF3I	CTNAG	3 cut(s)	1102, 1247, 1409
HpySE526I	ACGT	3 cut(s)	129, 309, 642
Hsp92I	GRCGYC	2 cut(s)	902, 1776
HspAI	GCGC	4 cut(s)	137, 546, 771, 1776
KasI	GGCGCC	1 cut(s)	1775
Ksp22I	TGATCA	1 cut(s)	396
KspAI	GTTAAC	1 cut(s)	1750
Kzo9I	GATC	6 cut(s)	396, 595, 1269, 1299, 1516, 1551
Lsp1109I	GCAGC	6 cut(s)	334, 373, 961, 1077, 1796, 1843
LweI	GCATC	4 cut(s)	163, 247, 961, 1597
MaeI	CTAG	4 cut(s)	584, 1007, 1361, 1854
MaeII	ACGT	3 cut(s)	129, 309, 642
MaeIII	GTNAC	5 cut(s)	6, 719, 748, 1078, 1344
MalI	GATC	6 cut(s)	398, 597, 1271, 1301, 1518, 1553
MboI	GATC	6 cut(s)	396, 595, 1269, 1299, 1516, 1551
MboII	GAAGA	6 cut(s)	49, 241, 272, 859, 1118, 1666
MflI	RGATCY	2 cut(s)	1269, 1551
MhlI	GDGCHC	3 cut(s)	608, 702, 937
MlsI	TGGCCA	1 cut(s)	1151
MluNI	TGGCCA	1 cut(s)	1151
Mly113I	GGCGCC	1 cut(s)	1776
Mox20I	TGGCCA	1 cut(s)	1151
Mph1103I	ATGCAT	1 cut(s)	498
MroXI	GAANNNNTTC	1 cut(s)	1571
MscI	TGGCCA	1 cut(s)	1151
MseI	TTAA	5 cut(s)	635, 926, 1449, 1521, 1749
MslI	CAYNNNNRTG	4 cut(s)	225, 377, 1403, 1480
Msp20I	TGGCCA	1 cut(s)	1151
MspCI	CTTAAG	1 cut(s)	1520
MspI	CCGG	4 cut(s)	414, 443, 1076, 1254
MspR9I	CCNGG	2 cut(s)	570, 703
MssI	GTTTAAAC	1 cut(s)	636
Mva1269I	GAATGC	1 cut(s)	1742
MvaI	CCWGG	2 cut(s)	570, 703
MvnI	CGCG	1 cut(s)	548
MwoI	GCNNNNNNNGC	3 cut(s)	300, 493, 779
NarI	GGCGCC	1 cut(s)	1776
NcoI	CCATGG	1 cut(s)	679
NdeI	CATATG	2 cut(s)	327, 1817
NdeII	GATC	6 cut(s)	396, 595, 1269, 1299, 1516, 1551
NlaIV	GGNNCC	3 cut(s)	1190, 1726, 1777
NmeAIII	GCCGAG	1 cut(s)	934
NmuCI	GTSAC	3 cut(s)	6, 748, 1078
NsiI	ATGCAT	1 cut(s)	498
NspI	RCATGY	3 cut(s)	796, 1210, 1355
OliI	CACNNNNGTG	1 cut(s)	377
PaeI	GCATGC	1 cut(s)	796
PaeR7I	CTCGAG	1 cut(s)	1091
PceI	AGGCCT	1 cut(s)	275
PciI	ACATGT	1 cut(s)	1206
PcsI	WCGNNNNNNNCGW	3 cut(s)	229, 249, 371
PctI	GAATGC	1 cut(s)	1742
PdmI	GAANNNNTTC	1 cut(s)	1571
PfeI	GAWTC	2 cut(s)	1072, 1310
PinAI	ACCGGT	1 cut(s)	1253
PkrI	GCNGC	9 cut(s)	324, 385, 388, 976, 1067, 1340, 1473, 1811, 1833
PluTI	GGCGCC	1 cut(s)	1779
PmeI	GTTTAAAC	1 cut(s)	636
PpuMI	RGGWCCY	2 cut(s)	1370, 1724
PscI	ACATGT	1 cut(s)	1206
PsiI	TTATAA	1 cut(s)	1437
Psp5II	RGGWCCY	2 cut(s)	1370, 1724
Psp6I	CCWGG	2 cut(s)	568, 701
PspEI	GGTNACC	1 cut(s)	1078
PspFI	CCCAGC	1 cut(s)	315
PspGI	CCWGG	2 cut(s)	568, 701
PspN4I	GGNNCC	3 cut(s)	1190, 1726, 1777
PspPI	GGNCC	3 cut(s)	19, 1370, 1724
PspPPI	RGGWCCY	2 cut(s)	1370, 1724
PsuI	RGATCY	2 cut(s)	1269, 1551
RsaI	GTAC	8 cut(s)	166, 764, 1175, 1220, 1226, 1258, 1319, 1420
RsaNI	GTAC	8 cut(s)	165, 763, 1174, 1219, 1225, 1257, 1318, 1419
RseI	CAYNNNNRTG	4 cut(s)	225, 377, 1403, 1480
SaqAI	TTAA	5 cut(s)	635, 926, 1449, 1521, 1749
SatI	GCNGC	9 cut(s)	323, 384, 387, 975, 1066, 1339, 1472, 1810, 1832
Sau3AI	GATC	6 cut(s)	396, 595, 1269, 1299, 1516, 1551
Sau96I	GGNCC	3 cut(s)	19, 1370, 1724
ScrFI	CCNGG	2 cut(s)	570, 703
SduI	GDGCHC	3 cut(s)	608, 702, 937
SfaNI	GCATC	4 cut(s)	163, 247, 961, 1597
SfcI	CTRYAG	1 cut(s)	1263
SfoI	GGCGCC	1 cut(s)	1777
Sfr274I	CTCGAG	1 cut(s)	1091
SinI	GGWCC	3 cut(s)	19, 1370, 1724
SlaI	CTCGAG	1 cut(s)	1091
SmiMI	CAYNNNNRTG	4 cut(s)	225, 377, 1403, 1480
SmlI	CTYRAG	3 cut(s)	871, 1091, 1520
SmoI	CTYRAG	3 cut(s)	871, 1091, 1520
SphI	GCATGC	1 cut(s)	796
SseBI	AGGCCT	1 cut(s)	275
SsiI	CCGC	6 cut(s)	383, 446, 548, 1338, 1472, 1740
SspDI	GGCGCC	1 cut(s)	1775
SspMI	CTAG	4 cut(s)	584, 1007, 1361, 1854
StuI	AGGCCT	1 cut(s)	275
StyD4I	CCNGG	2 cut(s)	568, 701
StyI	CCWWGG	1 cut(s)	679
TaaI	ACNGT	6 cut(s)	89, 287, 695, 1402, 1418, 1766
TaiI	ACGT	3 cut(s)	132, 312, 645
TaqI	TCGA	8 cut(s)	144, 258, 365, 598, 1092, 1184, 1527, 1605
TatI	WGTACW	2 cut(s)	1218, 1418
TauI	GCSGC	3 cut(s)	386, 1341, 1474
TfiI	GAWTC	2 cut(s)	1072, 1310
Tru1I	TTAA	5 cut(s)	635, 926, 1449, 1521, 1749
Tru9I	TTAA	5 cut(s)	635, 926, 1449, 1521, 1749
TscAI	CASTG	3 cut(s)	285, 305, 700
TseFI	GTSAC	3 cut(s)	6, 748, 1078
TseI	GCWGC	6 cut(s)	322, 386, 974, 1065, 1809, 1831
Tsp45I	GTSAC	3 cut(s)	6, 748, 1078
TspDTI	ATGAA	3 cut(s)	209, 1214, 1734
TspGWI	ACGGA	1 cut(s)	383
TspRI	CASTG	3 cut(s)	285, 305, 700
Vha464I	CTTAAG	1 cut(s)	1520
VpaK11BI	GGWCC	3 cut(s)	19, 1370, 1724
XagI	CCTNNNNNAGG	1 cut(s)	928
XapI	RAATTY	3 cut(s)	466, 725, 1594
XceI	RCATGY	3 cut(s)	796, 1210, 1355
XhoI	CTCGAG	1 cut(s)	1091
XmnI	GAANNNNTTC	1 cut(s)	1571
XspI	CTAG	4 cut(s)	584, 1007, 1361, 1854
Zsp2I	ATGCAT	1 cut(s)	498

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.