Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4A

Orthogroup ID

OG0006001

Physical Location & Seq

Genomic Coordinates Forward (+)

71877420 .. 71880747

Gene Structure 3328 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4AG422000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2079 bp

ATGTCGTCACATAATCTGGACATGAGTGGGAGCAAGAAGAGGAAGAGAGGCGAGAGGGTTTTCAGGTTCAAGACTTTTGGAGAAAACGGTTATCCAGTCGGGTTCGTTGCCTCGTCGCTTAGGGAAAACGTGAAAGCGCTTCTCGAATATGGGCATCTGGAGAGTGCCCTATGCAATAGTGCTACTGGAATAAAGAGCTGGTCGTTCCATCTCCAGGTTCATCGTAATGCTCTCGTGCATCTCGTTCTCTTCGTCGTCGAAGAACCTATTGAGGCCTCACTGAACCGTCATTGCAAGCAGTGCCAATACGTCGGCTGGGGGCAGCATATGGTTTGCAATAAGAAGTATCACTTTGTGTTGCCTTCGACCGACACGATGGTGGCGGCGGCTTGTTTGAACAACTGCAAAGTTGCCGGAGGCAATAACAACTACGATGATATTCCGGCGGGTAAGTCATCGTCGTTGAATTTACTGGAATTAGAGGGGCATATCATGCATGGTGTATTTCACTCTAACGGGTTCGGGCATTTGCTTTGTGTCAATGGGGCGGAAACGGGTTCGGACTTGCCTGGACATCAGATACTTGAGTTTTGGGATCGATTGTGCACGGGATTACGAGCAAGGAAAGTGAGTTTAAACGACGTTTCACATAAGAAAGGCATGGAGTTGAGGCTCATCCATGGAGTAGCATACAGTGAGCCCTGGTTTGGTCGTTGGGGTTACAAATTTGGCCGTGGAACATTTTGTGTCACTCAACAAATGTACCAAAGCGCCATTGAAGCATTACAAGGCATGCCCTTGTGCTTGCTCATCCACCATCTTGCTGACATTACTTGCAACCAATTAGGCCACGATATTTCCCTCATCTTCTCAAGGTATCACGCATTGTCGGATCATTCATTGGCGACTCTCGGTGACCTATTCCATTTCATGCTAGAGCTCAAGTCCCGGCTACCCACTGACGATCAAAACTGGTGCATTGATTCCTATAACCCGGGAATGCTGGTGGAGACATCTTGCAGATGGTCTCCTAAAAGAGTTGAAATGGCGTCTCGGGTGATTGTGGAAGCCTTAAAGAGGGCTCATTTCAGATGGGTCTCTAGGCAGGAAGTAAGGGATGCTGCTCGCTCGTATATAGGGGACACGGGCTTGCTAGACTTCGTGTTGAAGTCGTTGGGCAACCAAATTGTAGGGAATTACTTGGTTCGTCGCAGCTTGAATCCGGTGACCAAAGTTCTCGAGTATTGCTTAGAAGACCTTTCTAATGTGTCCCCTAACCAAGATGGCAATGTCATGACCAACACCAAAGTGAAGGGATGGTACAAGATTTCGAGGTTCCAGCTAATGAAGGACATGTTCTACTTGTACAAGTACATCCTCAAAGACCAAAAGCTAAGCACCGGTACTACTACAGGGATCTTTTCAGCAATTCCGATGGCTGTTAGGATCATTCTTGATTCCAAGTACCTTGTCAAAGATTACCGCGGCGAGTTACCGCATGTGAAACTCGAATTAGGACCTGAAGGAAAATCAAATCTCTATTGTACCGTAATGCTAAGAAACAGTACACAAGTGCTTGATTATAACAAGATGATTAACAACAACGAATTATTATTGCCGCCATACGAGTGTGTGACACTGAAAAACAATGCTACCATCAATGATCTTAAGCTCGAAGTGGAGAGGAACTTCAAGGAGATCTACTGGGGATTGAAAAGCTTCGTCGTGGAAACGATTGCGAATTTGAGTATCGATGCAAAGGGAACAGATATGGTTTTTGGGCTGGTTGAGGTGGGTGGAAAGATTGTGCTTGAAGGAAGCAACAAAGATATGAGAGGTGGTGGACTTGGACTTGTTAGAGAGCATGAAGGACCCATATTAGAATGCGGGTCGGTTAACTGCATTGTGGACTGTCCATGTGGTGCCAAAGATGACGACGGGGAAAGAATGGTGTGCTGCGACATATGTGAAGTGTGGCAGCATACTGTGTGTGTTAGAATTCCAAACAATGAACAAGTTCCTCATATATTTATCTGCAATCGATGCGAGAGAGAGATCGTACTTTTACCTTCTCCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007275 GO:0008150 GO:0009555 GO:0009653 GO:0009719 GO:0009725 GO:0009741 GO:0009791 GO:0009846 GO:0009856 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009987 GO:0010033 GO:0010208 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010927 GO:0010941 GO:0010942 GO:0014070 GO:0016043 GO:0019219 GO:0019222 GO:0022414 GO:0022607 GO:0030198 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0032870 GO:0032989 GO:0033993 GO:0042221 GO:0043062 GO:0043067 GO:0043068 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044085 GO:0044424 GO:0044464 GO:0044706 GO:0045229 GO:0045893 GO:0045935 GO:0048229 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048444 GO:0048448 GO:0048466 GO:0048518 GO:0048522 GO:0048608 GO:0048646 GO:0048653 GO:0048654 GO:0048655 GO:0048658 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051704 GO:0051716 GO:0055046 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0071310 GO:0071367 GO:0071396 GO:0071407 GO:0071495 GO:0071840 GO:0080090 GO:0085029 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0099402 GO:1901700 GO:1901701 GO:1902680 GO:1903506 GO:1903508 GO:1905392 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PHD

Protein Analysis

692

Amino Acids

77.99

Weight (kDa)

7.73

Isoelectric Point (pI)

34.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WHD_plant_repro	PF25874	341 - 419	3.7e-34	Winged helix-turn-helix domain in plant reproductive proteins
Ubiquitin_At1g33420	PF25565	509 - 607	2.5e-28	At1g33420-like, ubiquitin-like domain
PHD	PF00628	638 - 682	3.8e-07	PHD-finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0006001)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G22260
fragaria_vesca	FvH4_4g35820 FvH4_4g35830 FvH4_4g35830
malus_domestica	MD16G1012700.v1.1
prunus_persica	Prupe.1G338600_v2.0.a1
pyrus_communis	pycom16g01190
rosa_chinensis	RchiOBHm_Chr4g0445351 RchiOBHm_Chr4g0445361
rosa_laevigata	RLG00000005745 RLG00000005746
rosa_multiflora	Rmu_sc0000050.1_g000007 Rmu_sc0000050.1_g000008 Rmu_sc0000050.1_g000009
rosa_roxburghii	Rroxscaffold_5G00385870 Rroxscaffold_5G00385880
rosa_rugosa	Rorug04G0361000
rosa_samantha	Rh4AG422000 Rh4AG422100 Rh4BG434000 Rh4BG434100 Rh4CG448900 Rh4CG449000 Rh4DG430000 Rh4DG430100
rosa_wichuraiana	Rw4G036200 Rw4G036210

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1584
AccB1I	GGYRCC	1 cut(s)	1922
AccII	CGCG	1 cut(s)	1485
AciI	CCGC	9 cut(s)	383, 386, 446, 548, 1483, 1485, 1496, 1619, 1887
AclWI	GGATC	4 cut(s)	603, 900, 1424, 1454
AcoI	YGGCCR	1 cut(s)	730
AcsI	RAATTY	4 cut(s)	466, 725, 1741, 1998
AcuI	CTGAAG	1 cut(s)	1542
AcyI	GRCGYC	1 cut(s)	1049
AdeI	CACNNNGTG	1 cut(s)	355
AfaI	GTAC	9 cut(s)	764, 1322, 1367, 1373, 1405, 1466, 1546, 1567, 2061
AfeI	AGCGCT	1 cut(s)	138
AfiI	CCNNNNNNNGG	3 cut(s)	214, 707, 1077
AflII	CTTAAG	1 cut(s)	1667
AflIII	ACRYGT	1 cut(s)	1353
AgeI	ACCGGT	1 cut(s)	1400
AjnI	CCWGG	3 cut(s)	213, 568, 701
AleI	CACNNNNGTG	2 cut(s)	377, 1307
AluBI	AGCT	7 cut(s)	198, 940, 1215, 1342, 1393, 1672, 1719
AluI	AGCT	7 cut(s)	198, 940, 1215, 1342, 1393, 1672, 1719
Alw21I	GWGCWC	2 cut(s)	608, 942
Alw26I	GTCTC	4 cut(s)	1004, 1032, 1056, 1102
Alw44I	GTGCAC	1 cut(s)	604
AlwI	GGATC	4 cut(s)	603, 900, 1424, 1454
Ama87I	CYCGRG	3 cut(s)	994, 1053, 1238
Aor51HI	AGCGCT	1 cut(s)	138
AoxI	GGCC	3 cut(s)	273, 730, 847
ApaLI	GTGCAC	1 cut(s)	604
ApeKI	GCWGC	5 cut(s)	322, 1121, 1212, 1956, 1978
ApoI	RAATTY	4 cut(s)	466, 725, 1741, 1998
AsiGI	ACCGGT	1 cut(s)	1400
Asp700I	GAANNNNTTC	2 cut(s)	1718, 2016
AspLEI	GCGC	2 cut(s)	139, 773
AspS9I	GGNCC	2 cut(s)	1517, 1871
AsuC2I	CCSGG	3 cut(s)	949, 995, 996
AsuHPI	GGTGA	3 cut(s)	926, 1069, 1237
AvaI	CYCGRG	3 cut(s)	994, 1053, 1238
AvaII	GGWCC	2 cut(s)	1517, 1871
BaeGI	GKGCMC	2 cut(s)	169, 608
BaeI	ACNNNNGTAYC	4 cut(s)	572, 605, 746, 779
BanI	GGYRCC	1 cut(s)	1922
BanII	GRGCYC	3 cut(s)	702, 942, 1084
BarI	GAAGNNNNNNTAC	2 cut(s)	1304, 1336
BauI	CACGAG	1 cut(s)	233
BbsI	GAAGAC	1 cut(s)	1260
Bbv12I	GWGCWC	2 cut(s)	608, 942
BbvI	GCAGC	5 cut(s)	334, 1108, 1224, 1943, 1990
BccI	CCATC	9 cut(s)	216, 370, 825, 1017, 1086, 1277, 1311, 1429, 1664
BceAI	ACGGC	1 cut(s)	717
BciT130I	CCWGG	3 cut(s)	215, 570, 703
BcnI	CCSGG	3 cut(s)	949, 995, 996
BcoDI	GTCTC	4 cut(s)	1004, 1032, 1056, 1102
BfaI	CTAG	3 cut(s)	935, 1101, 1154
BfmI	CTRYAG	1 cut(s)	1410
BfoI	RGCGCY	2 cut(s)	140, 774
BfrI	CTTAAG	1 cut(s)	1667
BglII	AGATCT	1 cut(s)	1698
BisI	GCNGC	9 cut(s)	323, 384, 387, 1122, 1213, 1486, 1619, 1957, 1979
BlpI	GCTNAGC	1 cut(s)	1394
BlsI	GCNGC	9 cut(s)	324, 385, 388, 1123, 1214, 1487, 1620, 1958, 1980
Bme1390I	CCNGG	6 cut(s)	215, 570, 703, 949, 995, 996
Bme18I	GGWCC	2 cut(s)	1517, 1871
BmeT110I	CYCGRG	3 cut(s)	994, 1053, 1238
BmgT120I	GGNCC	2 cut(s)	1517, 1871
BmiI	GGNNCC	3 cut(s)	1337, 1873, 1924
BmrFI	CCNGG	6 cut(s)	215, 570, 703, 949, 995, 996
BmrI	ACTGGG	1 cut(s)	1714
BmsI	GCATC	5 cut(s)	163, 247, 1108, 1744, 2033
BmuI	ACTGGG	1 cut(s)	1714
BpiI	GAAGAC	1 cut(s)	1260
BpmI	CTGGAG	2 cut(s)	179, 197
Bpu10I	CCTNAGC	1 cut(s)	119
Bpu1102I	GCTNAGC	1 cut(s)	1394
BpuEI	CTTGAG	3 cut(s)	605, 856, 926
BpuMI	CCSGG	3 cut(s)	949, 995, 996
Bsa29I	ATCGAT	3 cut(s)	598, 1752, 2041
BsaHI	GRCGYC	1 cut(s)	1049
BsaI	GGTCTC	2 cut(s)	1032, 1102
BsaJI	CCNNGG	5 cut(s)	679, 701, 733, 994, 1483
BsaWI	WCCGGW	2 cut(s)	1222, 1400
Bsc4I	CCNNNNNNNGG	3 cut(s)	214, 707, 1077
Bse118I	RCCGGY	1 cut(s)	1400
Bse1I	ACTGG	5 cut(s)	95, 190, 477, 977, 1709
Bse3DI	GCAATG	2 cut(s)	289, 1294
BseBI	CCWGG	3 cut(s)	215, 570, 703
BseCI	ATCGAT	3 cut(s)	598, 1752, 2041
BseDI	CCNNGG	5 cut(s)	679, 701, 733, 994, 1483
BseGI	GGATG	5 cut(s)	675, 810, 1123, 1322, 1374
BseLI	CCNNNNNNNGG	3 cut(s)	214, 707, 1077
BseMI	GCAATG	2 cut(s)	289, 1294
BseNI	ACTGG	5 cut(s)	95, 190, 477, 977, 1709
BseSI	GKGCMC	2 cut(s)	169, 608
BseXI	GCAGC	5 cut(s)	334, 1108, 1224, 1943, 1990
BseYI	CCCAGC	1 cut(s)	315
Bsh1236I	CGCG	1 cut(s)	1485
Bsh1285I	CGRYCG	1 cut(s)	369
BshFI	GGCC	3 cut(s)	275, 732, 849
BshNI	GGYRCC	1 cut(s)	1922
BshTI	ACCGGT	1 cut(s)	1400
BshVI	ATCGAT	3 cut(s)	598, 1752, 2041
BsiEI	CGRYCG	1 cut(s)	369
BsiHKAI	GWGCWC	2 cut(s)	608, 942
BsiHKCI	CYCGRG	3 cut(s)	994, 1053, 1238
BsiSI	CCGG	6 cut(s)	414, 443, 949, 995, 1223, 1401
BslFI	GGGAC	3 cut(s)	931, 1154, 1255
BslI	CCNNNNNNNGG	3 cut(s)	214, 707, 1077
BsmAI	GTCTC	4 cut(s)	1004, 1032, 1056, 1102
BsmBI	CGTCTC	1 cut(s)	1056
BsmFI	GGGAC	3 cut(s)	931, 1154, 1255
BsmI	GAATGC	2 cut(s)	1005, 1889
BsnI	GGCC	3 cut(s)	275, 732, 849
Bso31I	GGTCTC	2 cut(s)	1032, 1102
BsoBI	CYCGRG	3 cut(s)	994, 1053, 1238
Bsp1286I	GDGCHC	5 cut(s)	169, 608, 702, 942, 1084
Bsp1407I	TGTACA	1 cut(s)	1365
Bsp143I	GATC	8 cut(s)	595, 892, 964, 1416, 1446, 1663, 1698, 2055
Bsp1720I	GCTNAGC	1 cut(s)	1394
Bsp19I	CCATGG	1 cut(s)	679
BspACI	CCGC	9 cut(s)	383, 386, 446, 548, 1483, 1485, 1496, 1619, 1887
BspANI	GGCC	3 cut(s)	275, 732, 849
BspDI	ATCGAT	3 cut(s)	598, 1752, 2041
BspFNI	CGCG	1 cut(s)	1485
BspHI	TCATGA	1 cut(s)	1293
BspLI	GGNNCC	3 cut(s)	1337, 1873, 1924
BspPI	GGATC	4 cut(s)	603, 900, 1424, 1454
BspT107I	GGYRCC	1 cut(s)	1922
BspTI	CTTAAG	1 cut(s)	1667
BspTNI	GGTCTC	2 cut(s)	1032, 1102
BsrDI	GCAATG	2 cut(s)	289, 1294
BsrFI	RCCGGY	1 cut(s)	1400
BsrGI	TGTACA	1 cut(s)	1365
BsrI	ACTGG	5 cut(s)	95, 190, 477, 977, 1709
BssAI	RCCGGY	1 cut(s)	1400
BssECI	CCNNGG	5 cut(s)	679, 701, 733, 994, 1483
BssMI	GATC	8 cut(s)	595, 892, 964, 1416, 1446, 1663, 1698, 2055
BssNI	GRCGYC	1 cut(s)	1049
BssSI	CACGAG	1 cut(s)	233
BssT1I	CCWWGG	1 cut(s)	679
Bst2BI	CACGAG	1 cut(s)	233
Bst2UI	CCWGG	3 cut(s)	215, 570, 703
Bst4CI	ACNGT	7 cut(s)	89, 287, 695, 1549, 1565, 1913, 1987
Bst6I	CTCTTC	3 cut(s)	32, 38, 254
BstACI	GRCGYC	1 cut(s)	1049
BstAFI	CTTAAG	1 cut(s)	1667
BstAPI	GCANNNNNTGC	3 cut(s)	300, 493, 2043
BstAUI	TGTACA	1 cut(s)	1365
BstC8I	GCNNGC	5 cut(s)	296, 794, 806, 1126, 1151
BstDEI	CTNAG	4 cut(s)	119, 1249, 1394, 1556
BstDSI	CCRYGG	3 cut(s)	679, 733, 1483
BstEII	GGTNACC	2 cut(s)	914, 1225
BstENI	CCTNNNNNAGG	1 cut(s)	1075
BstF5I	GGATG	5 cut(s)	675, 810, 1123, 1322, 1374
BstFNI	CGCG	1 cut(s)	1485
BstH2I	RGCGCY	2 cut(s)	140, 774
BstHHI	GCGC	2 cut(s)	139, 773
BstKTI	GATC	8 cut(s)	598, 895, 967, 1419, 1449, 1666, 1701, 2058
BstMAI	GTCTC	4 cut(s)	1004, 1032, 1056, 1102
BstMBI	GATC	8 cut(s)	595, 892, 964, 1416, 1446, 1663, 1698, 2055
BstMCI	CGRYCG	1 cut(s)	369
BstMWI	GCNNNNNNNGC	4 cut(s)	300, 493, 779, 2043
BstNI	CCWGG	3 cut(s)	215, 570, 703
BstNSI	RCATGY	3 cut(s)	796, 1357, 1502
BstPI	GGTNACC	2 cut(s)	914, 1225
BstSCI	CCNGG	6 cut(s)	213, 568, 701, 947, 993, 994
BstSFI	CTRYAG	1 cut(s)	1410
BstSLI	GKGCMC	2 cut(s)	169, 608
BstUI	CGCG	1 cut(s)	1485
BstV1I	GCAGC	5 cut(s)	334, 1108, 1224, 1943, 1990
BstV2I	GAAGAC	1 cut(s)	1260
BstX2I	RGATCY	2 cut(s)	1416, 1698
BstYI	RGATCY	2 cut(s)	1416, 1698
Bsu15I	ATCGAT	3 cut(s)	598, 1752, 2041
BsuRI	GGCC	3 cut(s)	275, 732, 849
BsuTUI	ATCGAT	3 cut(s)	598, 1752, 2041
BtgI	CCRYGG	3 cut(s)	679, 733, 1483
BtsCI	GGATG	5 cut(s)	675, 810, 1123, 1322, 1374
BtsI	GCAGTG	1 cut(s)	305
BtsIMutI	CAGTG	5 cut(s)	278, 305, 700, 957, 1637
Cac8I	GCNNGC	5 cut(s)	296, 794, 806, 1126, 1151
CciI	TCATGA	1 cut(s)	1293
CfoI	GCGC	2 cut(s)	139, 773
Cfr10I	RCCGGY	1 cut(s)	1400
Cfr13I	GGNCC	2 cut(s)	1517, 1871
Cfr42I	CCGCGG	1 cut(s)	1486
Cfr9I	CCCGGG	1 cut(s)	994
ClaI	ATCGAT	3 cut(s)	598, 1752, 2041
CseI	GACGC	1 cut(s)	1038
Csp6I	GTAC	9 cut(s)	763, 1321, 1366, 1372, 1404, 1465, 1545, 1566, 2060
CspAI	ACCGGT	1 cut(s)	1400
CviQI	GTAC	9 cut(s)	763, 1321, 1366, 1372, 1404, 1465, 1545, 1566, 2060
DdeI	CTNAG	4 cut(s)	119, 1249, 1394, 1556
DpnI	GATC	8 cut(s)	597, 894, 966, 1418, 1448, 1665, 1700, 2057
DpnII	GATC	8 cut(s)	595, 892, 964, 1416, 1446, 1663, 1698, 2055
DraI	TTTAAA	1 cut(s)	636
DraIII	CACNNNGTG	1 cut(s)	355
EaeI	YGGCCR	1 cut(s)	730
Eam1104I	CTCTTC	3 cut(s)	32, 38, 254
EarI	CTCTTC	3 cut(s)	32, 38, 254
EciI	GGCGGA	1 cut(s)	563
Ecl136II	GAGCTC	1 cut(s)	940
Eco130I	CCWWGG	1 cut(s)	679
Eco147I	AGGCCT	1 cut(s)	275
Eco24I	GRGCYC	3 cut(s)	702, 942, 1084
Eco31I	GGTCTC	2 cut(s)	1032, 1102
Eco47I	GGWCC	2 cut(s)	1517, 1871
Eco47III	AGCGCT	1 cut(s)	138
Eco53kI	GAGCTC	1 cut(s)	940
Eco57I	CTGAAG	1 cut(s)	1542
Eco88I	CYCGRG	3 cut(s)	994, 1053, 1238
Eco91I	GGTNACC	2 cut(s)	914, 1225
EcoICRI	GAGCTC	1 cut(s)	940
EcoNI	CCTNNNNNAGG	1 cut(s)	1075
EcoO109I	RGGNCCY	2 cut(s)	1517, 1871
EcoO65I	GGTNACC	2 cut(s)	914, 1225
EcoRI	GAATTC	1 cut(s)	1998
EcoRII	CCWGG	3 cut(s)	213, 568, 701
EcoT14I	CCWWGG	1 cut(s)	679
EcoT22I	ATGCAT	1 cut(s)	498
EcoT38I	GRGCYC	3 cut(s)	702, 942, 1084
ErhI	CCWWGG	1 cut(s)	679
Esp3I	CGTCTC	1 cut(s)	1056
FalI	AAGNNNNNCTT	2 cut(s)	335, 367
FaqI	GGGAC	3 cut(s)	931, 1154, 1255
FauI	CCCGC	2 cut(s)	439, 1880
FauNDI	CATATG	2 cut(s)	327, 1964
Fnu4HI	GCNGC	9 cut(s)	323, 384, 387, 1122, 1213, 1486, 1619, 1957, 1979
FokI	GGATG	5 cut(s)	662, 797, 1130, 1329, 1361
FriOI	GRGCYC	3 cut(s)	702, 942, 1084
Fsp4HI	GCNGC	9 cut(s)	323, 384, 387, 1122, 1213, 1486, 1619, 1957, 1979
FspBI	CTAG	3 cut(s)	935, 1101, 1154
GlaI	GCGC	2 cut(s)	138, 772
GluI	GCNGC	9 cut(s)	323, 384, 387, 1122, 1213, 1486, 1619, 1957, 1979
GsaI	CCCAGC	1 cut(s)	319
GsuI	CTGGAG	2 cut(s)	179, 197
HaeII	RGCGCY	2 cut(s)	140, 774
HaeIII	GGCC	3 cut(s)	275, 732, 849
HapII	CCGG	6 cut(s)	414, 443, 949, 995, 1223, 1401
HgaI	GACGC	1 cut(s)	1038
HhaI	GCGC	2 cut(s)	139, 773
Hin1I	GRCGYC	1 cut(s)	1049
Hin6I	GCGC	2 cut(s)	137, 771
HinP1I	GCGC	2 cut(s)	137, 771
HincII	GTYRAC	1 cut(s)	1897
HindII	GTYRAC	1 cut(s)	1897
HindIII	AAGCTT	1 cut(s)	1717
HinfI	GANTC	4 cut(s)	907, 983, 1219, 1457
HpaI	GTTAAC	1 cut(s)	1897
HpaII	CCGG	6 cut(s)	414, 443, 949, 995, 1223, 1401
HphI	GGTGA	3 cut(s)	926, 1069, 1237
Hpy166II	GTNNAC	5 cut(s)	606, 1568, 1844, 1897, 1909
Hpy188I	TCNGA	5 cut(s)	562, 579, 892, 1091, 1434
Hpy188III	TCNNGA	7 cut(s)	17, 70, 143, 158, 1238, 1294, 1454
Hpy8I	GTNNAC	5 cut(s)	606, 1568, 1844, 1897, 1909
Hpy99I	CGWCG	9 cut(s)	118, 257, 260, 314, 463, 644, 1212, 1727, 1940
HpyAV	CCTTC	7 cut(s)	372, 1306, 1342, 1517, 1808, 1862, 2079
HpyCH4III	ACNGT	7 cut(s)	89, 287, 695, 1549, 1565, 1913, 1987
HpyCH4IV	ACGT	3 cut(s)	129, 309, 642
HpyF10VI	GCNNNNNNNGC	4 cut(s)	300, 493, 779, 2043
HpyF3I	CTNAG	4 cut(s)	119, 1249, 1394, 1556
HpySE526I	ACGT	3 cut(s)	129, 309, 642
Hsp92I	GRCGYC	1 cut(s)	1049
HspAI	GCGC	2 cut(s)	137, 771
KspAI	GTTAAC	1 cut(s)	1897
KspI	CCGCGG	1 cut(s)	1486
Kzo9I	GATC	8 cut(s)	595, 892, 964, 1416, 1446, 1663, 1698, 2055
LmnI	GCTCC	1 cut(s)	30
Lsp1109I	GCAGC	5 cut(s)	334, 1108, 1224, 1943, 1990
LweI	GCATC	5 cut(s)	163, 247, 1108, 1744, 2033
MaeI	CTAG	3 cut(s)	935, 1101, 1154
MaeII	ACGT	3 cut(s)	129, 309, 642
MaeIII	GTNAC	7 cut(s)	6, 719, 748, 914, 1225, 1491, 1633
MalI	GATC	8 cut(s)	597, 894, 966, 1418, 1448, 1665, 1700, 2057
MboI	GATC	8 cut(s)	595, 892, 964, 1416, 1446, 1663, 1698, 2055
MboII	GAAGA	6 cut(s)	49, 55, 241, 272, 859, 1265
MflI	RGATCY	2 cut(s)	1416, 1698
MhlI	GDGCHC	5 cut(s)	169, 608, 702, 942, 1084
MlyI	GAGTC	1 cut(s)	901
MmeI	TCCRAC	1 cut(s)	870
Mph1103I	ATGCAT	1 cut(s)	498
MroXI	GAANNNNTTC	2 cut(s)	1718, 2016
MseI	TTAA	5 cut(s)	635, 1073, 1596, 1668, 1896
MslI	CAYNNNNRTG	4 cut(s)	225, 377, 1307, 1627
MspA1I	CMGCKG	1 cut(s)	1485
MspCI	CTTAAG	1 cut(s)	1667
MspI	CCGG	6 cut(s)	414, 443, 949, 995, 1223, 1401
MspR9I	CCNGG	6 cut(s)	215, 570, 703, 949, 995, 996
MssI	GTTTAAAC	1 cut(s)	636
Mva1269I	GAATGC	2 cut(s)	1005, 1889
MvaI	CCWGG	3 cut(s)	215, 570, 703
MvnI	CGCG	1 cut(s)	1485
MwoI	GCNNNNNNNGC	4 cut(s)	300, 493, 779, 2043
NciI	CCSGG	3 cut(s)	949, 995, 996
NcoI	CCATGG	1 cut(s)	679
NdeI	CATATG	2 cut(s)	327, 1964
NdeII	GATC	8 cut(s)	595, 892, 964, 1416, 1446, 1663, 1698, 2055
NlaIV	GGNNCC	3 cut(s)	1337, 1873, 1924
NmuCI	GTSAC	5 cut(s)	6, 748, 914, 1225, 1633
NsiI	ATGCAT	1 cut(s)	498
NspI	RCATGY	3 cut(s)	796, 1357, 1502
OliI	CACNNNNGTG	2 cut(s)	377, 1307
PaeI	GCATGC	1 cut(s)	796
PaeR7I	CTCGAG	1 cut(s)	1238
PagI	TCATGA	1 cut(s)	1293
PceI	AGGCCT	1 cut(s)	275
PciI	ACATGT	1 cut(s)	1353
PcsI	WCGNNNNNNNCGW	3 cut(s)	240, 249, 371
PctI	GAATGC	2 cut(s)	1005, 1889
PdmI	GAANNNNTTC	2 cut(s)	1718, 2016
PfeI	GAWTC	3 cut(s)	983, 1219, 1457
PinAI	ACCGGT	1 cut(s)	1400
PkrI	GCNGC	9 cut(s)	324, 385, 388, 1123, 1214, 1487, 1620, 1958, 1980
PleI	GAGTC	1 cut(s)	901
PmeI	GTTTAAAC	1 cut(s)	636
PpsI	GAGTC	1 cut(s)	901
PpuMI	RGGWCCY	2 cut(s)	1517, 1871
PscI	ACATGT	1 cut(s)	1353
PsiI	TTATAA	1 cut(s)	1584
Psp124BI	GAGCTC	1 cut(s)	942
Psp5II	RGGWCCY	2 cut(s)	1517, 1871
Psp6I	CCWGG	3 cut(s)	213, 568, 701
PspEI	GGTNACC	2 cut(s)	914, 1225
PspFI	CCCAGC	1 cut(s)	315
PspGI	CCWGG	3 cut(s)	213, 568, 701
PspN4I	GGNNCC	3 cut(s)	1337, 1873, 1924
PspPI	GGNCC	2 cut(s)	1517, 1871
PspPPI	RGGWCCY	2 cut(s)	1517, 1871
PsuI	RGATCY	2 cut(s)	1416, 1698
RsaI	GTAC	9 cut(s)	764, 1322, 1367, 1373, 1405, 1466, 1546, 1567, 2061
RsaNI	GTAC	9 cut(s)	763, 1321, 1366, 1372, 1404, 1465, 1545, 1566, 2060
RseI	CAYNNNNRTG	4 cut(s)	225, 377, 1307, 1627
SacI	GAGCTC	1 cut(s)	942
SacII	CCGCGG	1 cut(s)	1486
SaqAI	TTAA	5 cut(s)	635, 1073, 1596, 1668, 1896
SatI	GCNGC	9 cut(s)	323, 384, 387, 1122, 1213, 1486, 1619, 1957, 1979
Sau3AI	GATC	8 cut(s)	595, 892, 964, 1416, 1446, 1663, 1698, 2055
Sau96I	GGNCC	2 cut(s)	1517, 1871
SchI	GAGTC	1 cut(s)	901
ScrFI	CCNGG	6 cut(s)	215, 570, 703, 949, 995, 996
SduI	GDGCHC	5 cut(s)	169, 608, 702, 942, 1084
SfaNI	GCATC	5 cut(s)	163, 247, 1108, 1744, 2033
SfcI	CTRYAG	1 cut(s)	1410
Sfr274I	CTCGAG	1 cut(s)	1238
Sfr303I	CCGCGG	1 cut(s)	1486
SgrBI	CCGCGG	1 cut(s)	1486
SinI	GGWCC	2 cut(s)	1517, 1871
SlaI	CTCGAG	1 cut(s)	1238
SmaI	CCCGGG	1 cut(s)	996
SmiMI	CAYNNNNRTG	4 cut(s)	225, 377, 1307, 1627
SmlI	CTYRAG	5 cut(s)	584, 871, 941, 1238, 1667
SmoI	CTYRAG	5 cut(s)	584, 871, 941, 1238, 1667
SphI	GCATGC	1 cut(s)	796
SseBI	AGGCCT	1 cut(s)	275
SsiI	CCGC	9 cut(s)	383, 386, 446, 548, 1483, 1485, 1496, 1619, 1887
SspMI	CTAG	3 cut(s)	935, 1101, 1154
SstI	GAGCTC	1 cut(s)	942
StuI	AGGCCT	1 cut(s)	275
StyD4I	CCNGG	6 cut(s)	213, 568, 701, 947, 993, 994
StyI	CCWWGG	1 cut(s)	679
TaaI	ACNGT	7 cut(s)	89, 287, 695, 1549, 1565, 1913, 1987
TaiI	ACGT	3 cut(s)	132, 312, 645
TaqII	GACCGA	1 cut(s)	383
TatI	WGTACW	3 cut(s)	1365, 1371, 1565
TauI	GCSGC	4 cut(s)	386, 389, 1488, 1621
TfiI	GAWTC	3 cut(s)	983, 1219, 1457
Tru1I	TTAA	5 cut(s)	635, 1073, 1596, 1668, 1896
Tru9I	TTAA	5 cut(s)	635, 1073, 1596, 1668, 1896
TscAI	CASTG	5 cut(s)	285, 305, 700, 964, 1644
TseFI	GTSAC	5 cut(s)	6, 748, 914, 1225, 1633
TseI	GCWGC	5 cut(s)	322, 1121, 1212, 1956, 1978
Tsp45I	GTSAC	5 cut(s)	6, 748, 914, 1225, 1633
TspDTI	ATGAA	6 cut(s)	209, 888, 919, 1361, 1881, 2025
TspMI	CCCGGG	1 cut(s)	994
TspRI	CASTG	5 cut(s)	285, 305, 700, 964, 1644
Vha464I	CTTAAG	1 cut(s)	1667
VneI	GTGCAC	1 cut(s)	604
VpaK11BI	GGWCC	2 cut(s)	1517, 1871
XagI	CCTNNNNNAGG	1 cut(s)	1075
XapI	RAATTY	4 cut(s)	466, 725, 1741, 1998
XceI	RCATGY	3 cut(s)	796, 1357, 1502
XhoI	CTCGAG	1 cut(s)	1238
XmaI	CCCGGG	1 cut(s)	994
XmnI	GAANNNNTTC	2 cut(s)	1718, 2016
XspI	CTAG	3 cut(s)	935, 1101, 1154
Zsp2I	ATGCAT	1 cut(s)	498

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.