FvH4_4g35920

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0009989
Physical Location & Seq
Reverse (-)
33058909 .. 33061009
2101 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g35920.t1

Sequence Viewer

Length: 903 bp
ATGGGTGAGCTTCACTCCATGTCCATGGCTTACTCTAGCAACAGCTTAGCCTTAGGAGGACCAGGAGGACTGGGTCTTGGTCTAGGACTGGGAGGAGGGAATAATTGGGATAATTGCCATAACTTCAACCTTGGAGTTCTCAGCTCGGACCGGATGTCTTTAGTGATGGATGACTTCGATGCGCCACCATTTTCCTCTACCCTGGAGTCGGATTTCTCGACCGGTTACTTGGAAGACGCTTTGGTCGAATTCAGCGAGCGCTCGAAAAGAAGACGCATGCTCTTGTTTAACGATGACAAGATCAGATCAGACTCTACTGCTTCTGCTTCAAAGGCATACTGGAATTCAGACTGGGAAATGTCTGAGAGTTTTGACAGCATCAGCCAACTAATCACCTCTGCTGGGCTATCAGGTGATGATGATCCTGCAAGTATGAGTAATACGCCAATTAGTAAAATCTATGGGGATGAGACAAACATAGCCGACACGAAGATGAACACAGGAGAAGAAGCTATTGATTCCTCGTCTTCATCTTCTTCTTGCAAAGAATCAGCCAACACCAACTCCATGGCTGCCGAGAAAGAAAGCCCCTTTTCCGTCGATGCTCTTCCCGCAGATGAGAAGAGAAAGAAGAAGAGGGCGGTAATGACGTCGAGGGTGGTGTATCCCTTTGCGGTGGTGAAGCCGGGAGGAGTAGAAGGTGACGTGACGCTAAACGACATTAACGAGAGGATTTCAATGCCACCAACGCGACCAGTACGACACCCAGTTGGGGACTTTGCGTGTCGGCCATGCGTATCTGATCCTGATGGTCCTGGACTTTCTGGTAAAGCTGTGGTGGCCCTTACCAGAATTCAAACACAGGGACGAGGCACGATCACTATTATCAGAACCAGAGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

301

Amino Acids

32.11

Weight (kDa)

4.84

Isoelectric Point (pI)

51.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009989)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14630 AT1G14630 AT2G01990 AT2G01990
fragaria_vesca FvH4_4g35920
malus_domestica MD13G1013900.v1.1 MD16G1011100.v1.1
prunus_persica Prupe.1G339600_v2.0.a1 Prupe.1G339600_v2.0.a1
pyrus_communis pycom13g01180 pycom16g01090
rosa_chinensis RchiOBHm_Chr4g0445521
rosa_laevigata RLG00000005733
rosa_multiflora Rmu_ssc0000253.1_g000002
rosa_roxburghii Rroxscaffold_5G00386000
rosa_rugosa Rorug04G0362100
rosa_samantha Rh4AG423600 Rh4BG435600 Rh4CG450300 Rh4DG431900
rosa_wichuraiana Rw4G036310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 242
AatII GACGTC 1 cut(s) 653
AccII CGCG 1 cut(s) 751
AciI CCGC 3 cut(s) 612, 641, 674
AclWI GGATC 2 cut(s) 416, 797
AcoI YGGCCR 1 cut(s) 788
AcsI RAATTY 3 cut(s) 248, 343, 852
AcyI GRCGYC 1 cut(s) 650
AfaI GTAC 1 cut(s) 759
AfeI AGCGCT 1 cut(s) 260
AfiI CCNNNNNNNGG 3 cut(s) 208, 402, 772
AgeI ACCGGT 1 cut(s) 221
AgsI TTSAA 4 cut(s) 127, 330, 738, 857
AhdI GACNNNNNGTC 1 cut(s) 154
AjiI CACGTC 1 cut(s) 706
AjnI CCWGG 3 cut(s) 61, 201, 814
AluBI AGCT 5 cut(s) 10, 45, 144, 512, 833
AluI AGCT 5 cut(s) 10, 45, 144, 512, 833
Alw26I GTCTC 1 cut(s) 464
AlwI GGATC 2 cut(s) 416, 797
Aor51HI AGCGCT 1 cut(s) 260
AoxI GGCC 2 cut(s) 788, 840
ApeKI GCWGC 1 cut(s) 572
ApoI RAATTY 3 cut(s) 248, 343, 852
AsiGI ACCGGT 1 cut(s) 221
AspLEI GCGC 2 cut(s) 184, 261
AspS9I GGNCC 4 cut(s) 59, 148, 812, 841
AsuC2I CCSGG 1 cut(s) 687
AsuHPI GGTGA 5 cut(s) 17, 385, 425, 691, 713
AvaII GGWCC 3 cut(s) 59, 148, 812
AxyI CCTNAGG 1 cut(s) 52
BbsI GAAGAC 3 cut(s) 240, 277, 519
BbvI GCAGC 1 cut(s) 559
BccI CCATC 2 cut(s) 160, 803
BciT130I CCWGG 3 cut(s) 63, 203, 816
BciVI GTATCC 1 cut(s) 675
BcnI CCSGG 1 cut(s) 687
BcoDI GTCTC 1 cut(s) 464
BfaI CTAG 3 cut(s) 36, 83, 901
BfoI RGCGCY 1 cut(s) 262
BfuI GTATCC 1 cut(s) 675
BisI GCNGC 1 cut(s) 573
BlpI GCTNAGC 1 cut(s) 46
BlsI GCNGC 1 cut(s) 574
Bme1390I CCNGG 4 cut(s) 63, 203, 687, 816
Bme18I GGWCC 3 cut(s) 59, 148, 812
BmeRI GACNNNNNGTC 1 cut(s) 154
BmgBI CACGTC 1 cut(s) 706
BmgT120I GGNCC 4 cut(s) 59, 148, 812, 841
BmrFI CCNGG 4 cut(s) 63, 203, 687, 816
BmrI ACTGGG 4 cut(s) 80, 98, 361, 761
BmsI GCATC 3 cut(s) 169, 387, 592
BmuI ACTGGG 4 cut(s) 80, 98, 361, 761
BpiI GAAGAC 3 cut(s) 240, 277, 519
BplI GAGNNNNNCTC 1 cut(s) 31
BpmI CTGGAG 1 cut(s) 224
Bpu1102I GCTNAGC 1 cut(s) 46
BpuMI CCSGG 1 cut(s) 687
BsaBI GATNNNNATC 1 cut(s) 420
BsaHI GRCGYC 1 cut(s) 650
BsaJI CCNNGG 4 cut(s) 24, 130, 201, 567
BsaWI WCCGGW 2 cut(s) 150, 221
BsaXI ACNNNNNCTCC 2 cut(s) 548, 578
Bsc4I CCNNNNNNNGG 3 cut(s) 208, 402, 772
Bse118I RCCGGY 1 cut(s) 221
Bse1I ACTGG 6 cut(s) 75, 93, 344, 356, 755, 767
Bse21I CCTNAGG 1 cut(s) 52
Bse8I GATNNNNATC 1 cut(s) 420
BseBI CCWGG 3 cut(s) 63, 203, 816
BseDI CCNNGG 4 cut(s) 24, 130, 201, 567
BseGI GGATG 3 cut(s) 159, 175, 472
BseJI GATNNNNATC 1 cut(s) 420
BseLI CCNNNNNNNGG 3 cut(s) 208, 402, 772
BseMII CTCAG 2 cut(s) 154, 354
BseNI ACTGG 6 cut(s) 75, 93, 344, 356, 755, 767
BseRI GAGGAG 2 cut(s) 108, 705
BseXI GCAGC 1 cut(s) 559
BseYI CCCAGC 1 cut(s) 401
Bsh1236I CGCG 1 cut(s) 751
Bsh1285I CGRYCG 1 cut(s) 222
BshFI GGCC 2 cut(s) 790, 842
BshTI ACCGGT 1 cut(s) 221
BsiEI CGRYCG 1 cut(s) 222
BsiSI CCGG 3 cut(s) 151, 222, 686
BslFI GGGAC 2 cut(s) 788, 879
BslI CCNNNNNNNGG 3 cut(s) 208, 402, 772
BsmAI GTCTC 1 cut(s) 464
BsmFI GGGAC 2 cut(s) 788, 879
BsnI GGCC 2 cut(s) 790, 842
Bsp143I GATC 5 cut(s) 300, 305, 421, 802, 876
Bsp1720I GCTNAGC 1 cut(s) 46
Bsp19I CCATGG 2 cut(s) 24, 567
BspACI CCGC 3 cut(s) 612, 641, 674
BspANI GGCC 2 cut(s) 790, 842
BspCNI CTCAG 2 cut(s) 153, 355
BspFNI CGCG 1 cut(s) 751
BspPI GGATC 2 cut(s) 416, 797
BspQI GCTCTTC 1 cut(s) 612
BsrFI RCCGGY 1 cut(s) 221
BsrI ACTGG 6 cut(s) 75, 93, 344, 356, 755, 767
BssAI RCCGGY 1 cut(s) 221
BssECI CCNNGG 4 cut(s) 24, 130, 201, 567
BssMI GATC 5 cut(s) 300, 305, 421, 802, 876
BssNI GRCGYC 1 cut(s) 650
BssT1I CCWWGG 3 cut(s) 24, 130, 567
Bst2UI CCWGG 3 cut(s) 63, 203, 816
Bst6I CTCTTC 3 cut(s) 612, 617, 629
BstACI GRCGYC 1 cut(s) 650
BstC8I GCNNGC 2 cut(s) 257, 278
BstDEI CTNAG 4 cut(s) 46, 52, 140, 363
BstDSI CCRYGG 2 cut(s) 24, 567
BstF5I GGATG 3 cut(s) 159, 175, 472
BstFNI CGCG 1 cut(s) 751
BstH2I RGCGCY 1 cut(s) 262
BstHHI GCGC 2 cut(s) 184, 261
BstKTI GATC 5 cut(s) 303, 308, 424, 805, 879
BstMAI GTCTC 1 cut(s) 464
BstMBI GATC 5 cut(s) 300, 305, 421, 802, 876
BstMCI CGRYCG 1 cut(s) 222
BstMWI GCNNNNNNNGC 4 cut(s) 332, 611, 748, 839
BstNI CCWGG 3 cut(s) 63, 203, 816
BstNSI RCATGY 1 cut(s) 280
BstSCI CCNGG 4 cut(s) 61, 201, 685, 814
BstUI CGCG 1 cut(s) 751
BstV1I GCAGC 1 cut(s) 559
BstV2I GAAGAC 3 cut(s) 240, 277, 519
BstXI CCANNNNNNTGG 2 cut(s) 25, 568
Bsu36I CCTNAGG 1 cut(s) 52
BsuI GTATCC 1 cut(s) 675
BsuRI GGCC 2 cut(s) 790, 842
BtgI CCRYGG 2 cut(s) 24, 567
BtrI CACGTC 1 cut(s) 706
BtsCI GGATG 3 cut(s) 159, 175, 472
Cac8I GCNNGC 2 cut(s) 257, 278
CfoI GCGC 2 cut(s) 184, 261
Cfr10I RCCGGY 1 cut(s) 221
Cfr13I GGNCC 4 cut(s) 59, 148, 812, 841
CpoI CGGWCCG 1 cut(s) 148
CseI GACGC 3 cut(s) 245, 282, 718
Csp6I GTAC 1 cut(s) 758
CspAI ACCGGT 1 cut(s) 221
CspI CGGWCCG 1 cut(s) 148
CviAII CATG 5 cut(s) 19, 25, 277, 568, 792
CviQI GTAC 1 cut(s) 758
DdeI CTNAG 4 cut(s) 46, 52, 140, 363
DpnI GATC 5 cut(s) 302, 307, 423, 804, 878
DpnII GATC 5 cut(s) 300, 305, 421, 802, 876
DrdI GACNNNNNNGTC 1 cut(s) 242
DriI GACNNNNNGTC 1 cut(s) 154
DseDI GACNNNNNNGTC 1 cut(s) 242
EaeI YGGCCR 1 cut(s) 788
Eam1104I CTCTTC 3 cut(s) 612, 617, 629
Eam1105I GACNNNNNGTC 1 cut(s) 154
EarI CTCTTC 3 cut(s) 612, 617, 629
Eco130I CCWWGG 3 cut(s) 24, 130, 567
Eco47I GGWCC 3 cut(s) 59, 148, 812
Eco47III AGCGCT 1 cut(s) 260
Eco81I CCTNAGG 1 cut(s) 52
EcoRI GAATTC 3 cut(s) 248, 343, 852
EcoRII CCWGG 3 cut(s) 61, 201, 814
EcoT14I CCWWGG 3 cut(s) 24, 130, 567
ErhI CCWWGG 3 cut(s) 24, 130, 567
FaeI CATG 5 cut(s) 22, 28, 280, 571, 795
FaqI GGGAC 2 cut(s) 788, 879
FatI CATG 5 cut(s) 18, 24, 276, 567, 791
FauI CCCGC 1 cut(s) 619
Fnu4HI GCNGC 1 cut(s) 573
FokI GGATG 3 cut(s) 166, 182, 479
Fsp4HI GCNGC 1 cut(s) 573
FspBI CTAG 3 cut(s) 36, 83, 901
GlaI GCGC 2 cut(s) 183, 260
GluI GCNGC 1 cut(s) 573
GsaI CCCAGC 1 cut(s) 405
GsuI CTGGAG 1 cut(s) 224
HaeII RGCGCY 1 cut(s) 262
HaeIII GGCC 2 cut(s) 790, 842
HapII CCGG 3 cut(s) 151, 222, 686
HgaI GACGC 3 cut(s) 245, 282, 718
HhaI GCGC 2 cut(s) 184, 261
Hin1I GRCGYC 1 cut(s) 650
Hin1II CATG 5 cut(s) 22, 28, 280, 571, 795
Hin6I GCGC 2 cut(s) 182, 259
HinP1I GCGC 2 cut(s) 182, 259
HinfI GANTC 4 cut(s) 206, 311, 518, 548
HpaII CCGG 3 cut(s) 151, 222, 686
HphI GGTGA 5 cut(s) 17, 385, 425, 691, 713
Hpy188I TCNGA 8 cut(s) 148, 211, 305, 310, 349, 364, 802, 890
Hpy188III TCNNGA 2 cut(s) 217, 806
Hpy99I CGWCG 2 cut(s) 602, 655
HpyAV CCTTC 1 cut(s) 692
HpyCH4IV ACGT 2 cut(s) 650, 705
HpyCH4V TGCA 2 cut(s) 428, 543
HpyF10VI GCNNNNNNNGC 4 cut(s) 332, 611, 748, 839
HpyF3I CTNAG 4 cut(s) 46, 52, 140, 363
HpySE526I ACGT 2 cut(s) 650, 705
Hsp92I GRCGYC 1 cut(s) 650
Hsp92II CATG 5 cut(s) 22, 28, 280, 571, 795
HspAI GCGC 2 cut(s) 182, 259
Kzo9I GATC 5 cut(s) 300, 305, 421, 802, 876
LguI GCTCTTC 1 cut(s) 612
Lsp1109I GCAGC 1 cut(s) 559
LweI GCATC 3 cut(s) 169, 387, 592
MaeI CTAG 3 cut(s) 36, 83, 901
MaeII ACGT 2 cut(s) 650, 705
MaeIII GTNAC 3 cut(s) 224, 701, 706
MalI GATC 5 cut(s) 302, 307, 423, 804, 878
MboI GATC 5 cut(s) 300, 305, 421, 802, 876
MluCI AATT 6 cut(s) 103, 112, 248, 343, 447, 852
MlyI GAGTC 2 cut(s) 215, 305
MmeI TCCRAC 1 cut(s) 189
MseI TTAA 2 cut(s) 288, 723
MslI CAYNNNNRTG 2 cut(s) 23, 491
MspI CCGG 3 cut(s) 151, 222, 686
MspR9I CCNGG 4 cut(s) 63, 203, 687, 816
MvaI CCWGG 3 cut(s) 63, 203, 816
MvnI CGCG 1 cut(s) 751
MwoI GCNNNNNNNGC 4 cut(s) 332, 611, 748, 839
NciI CCSGG 1 cut(s) 687
NcoI CCATGG 2 cut(s) 24, 567
NdeII GATC 5 cut(s) 300, 305, 421, 802, 876
NlaIII CATG 5 cut(s) 22, 28, 280, 571, 795
NmeAIII GCCGAG 1 cut(s) 601
NmuCI GTSAC 2 cut(s) 701, 706
NspI RCATGY 1 cut(s) 280
PaeI GCATGC 1 cut(s) 280
PciSI GCTCTTC 1 cut(s) 612
PcsI WCGNNNNNNNCGW 4 cut(s) 215, 243, 252, 723
PfeI GAWTC 2 cut(s) 518, 548
PflFI GACNNNGTC 1 cut(s) 72
PfoI TCCNGGA 1 cut(s) 814
PinAI ACCGGT 1 cut(s) 221
PkrI GCNGC 1 cut(s) 574
PleI GAGTC 2 cut(s) 214, 305
PpsI GAGTC 2 cut(s) 214, 305
Psp6I CCWGG 3 cut(s) 61, 201, 814
PspFI CCCAGC 1 cut(s) 401
PspGI CCWGG 3 cut(s) 61, 201, 814
PspPI GGNCC 4 cut(s) 59, 148, 812, 841
PsyI GACNNNGTC 1 cut(s) 72
RsaI GTAC 1 cut(s) 759
RsaNI GTAC 1 cut(s) 758
RseI CAYNNNNRTG 2 cut(s) 23, 491
Rsr2I CGGWCCG 1 cut(s) 148
RsrII CGGWCCG 1 cut(s) 148
SapI GCTCTTC 1 cut(s) 612
SaqAI TTAA 2 cut(s) 288, 723
SatI GCNGC 1 cut(s) 573
Sau3AI GATC 5 cut(s) 300, 305, 421, 802, 876
Sau96I GGNCC 4 cut(s) 59, 148, 812, 841
SchI GAGTC 2 cut(s) 215, 305
ScrFI CCNGG 4 cut(s) 63, 203, 687, 816
SfaNI GCATC 3 cut(s) 169, 387, 592
SinI GGWCC 3 cut(s) 59, 148, 812
SmiMI CAYNNNNRTG 2 cut(s) 23, 491
SphI GCATGC 1 cut(s) 280
Sse9I AATT 6 cut(s) 103, 112, 248, 343, 447, 852
SsiI CCGC 3 cut(s) 612, 641, 674
SspMI CTAG 3 cut(s) 36, 83, 901
StyD4I CCNGG 4 cut(s) 61, 201, 685, 814
StyI CCWWGG 3 cut(s) 24, 130, 567
TaiI ACGT 2 cut(s) 653, 708
TaqI TCGA 6 cut(s) 177, 218, 246, 263, 600, 653
TasI AATT 6 cut(s) 103, 112, 248, 343, 447, 852
TfiI GAWTC 2 cut(s) 518, 548
Tru1I TTAA 2 cut(s) 288, 723
Tru9I TTAA 2 cut(s) 288, 723
TseFI GTSAC 2 cut(s) 701, 706
TseI GCWGC 1 cut(s) 572
Tsp45I GTSAC 2 cut(s) 701, 706
TspDTI ATGAA 2 cut(s) 509, 519
TspGWI ACGGA 1 cut(s) 586
Tth111I GACNNNGTC 1 cut(s) 72
VpaK11BI GGWCC 3 cut(s) 59, 148, 812
XapI RAATTY 3 cut(s) 248, 343, 852
XceI RCATGY 1 cut(s) 280
XspI CTAG 3 cut(s) 36, 83, 901
ZraI GACGTC 1 cut(s) 651
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.