MD13G1013900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0009989
Physical Location & Seq
Forward (+)
861001 .. 862806
1806 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1013900.v1.1.491

Sequence Viewer

Length: 918 bp
ATGGGTGAGCTTCATCACTCCATGCCCATGGCTTACACGAACAGTAGCCTCCACAGCTCTTCCCAAGGTTGGGATTGTCACGGCCACCTTGGAGTTCTCAACACAGACAGAATGTCTTTAGCTATGGATGAGTACATGATGATGAACGCAGCATTTTGCTCATCACCTCATGTAGATCAGTCAGATTTCTCGACGGGGTACTTAGAAGACGCCTTGGTAGAATTTGGTGAGCGATCCAAAAGGAGACGCTTGCTCGTGTATACCAATGATGATAACGAGCTCAAAGACTCCACTACTATTCTTGCGAAGAGAAACTGGAATTCAAACTGGGAATTATCTGAGAATTTTGATTGCATGAGCCAGCTAACTACTTCCAGTACTGGGCTATCAGTTCTACTTGGTGATCCTTTATGTATAACCAGACCAATGAACAGACTCGTTTCCGAGGAGATCACAAATGACATCGTCGACTCAAAGATAAACACTCCAGAAGAGCCAACAATGTCAGCTCCTGAAGCTTTTGATTCCTCCTCTTCTTCTTGCAAAGATTCGACCAACACCAACTCTGATTATTTACCTACTCCCAAAGAAACCCTTTTTTCCAATATTGCTGCTCCAGCTGCCGGTGGTGGTGGTGATGAAAAGAGAAAAAAGAAGAAGAGGGTGATAACAAGGGTAGTTTATCCATTTGCGTTGGTGAAGCCCGGCGGAGTAGAAGGCGACGTCACCTTAAGCGACATCAACGAGAGGATCCTAATGCCACCAACACGGCCAGTACGACACCCAGTTGGGGACTTTGCATGTCGGCCATGCGTGTCTGCGGATGGCCCTGGCCTTTCTGGCAAAGCTGTGGTGGCCCTTACCAGAATTCAAACCCAGGGCAGAGGCACAATCACTATTATCAGAACCAAAGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

306

Amino Acids

33.37

Weight (kDa)

5.69

Isoelectric Point (pI)

52.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009989)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14630 AT1G14630 AT2G01990 AT2G01990
fragaria_vesca FvH4_4g35920
malus_domestica MD13G1013900.v1.1 MD16G1011100.v1.1
prunus_persica Prupe.1G339600_v2.0.a1 Prupe.1G339600_v2.0.a1
pyrus_communis pycom13g01180 pycom16g01090
rosa_chinensis RchiOBHm_Chr4g0445521
rosa_laevigata RLG00000005733
rosa_multiflora Rmu_ssc0000253.1_g000002
rosa_roxburghii Rroxscaffold_5G00386000
rosa_rugosa Rorug04G0362100
rosa_samantha Rh4AG423600 Rh4BG435600 Rh4CG450300 Rh4DG431900
rosa_wichuraiana Rw4G036310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 726
AccI GTMKAC 2 cut(s) 260, 468
AciI CCGC 2 cut(s) 708, 821
AclWI GGATC 4 cut(s) 228, 398, 745, 758
AcoI YGGCCR 3 cut(s) 82, 770, 806
AcsI RAATTY 4 cut(s) 221, 319, 343, 867
AcuI CTGAAG 1 cut(s) 534
AcyI GRCGYC 2 cut(s) 210, 723
AfaI GTAC 4 cut(s) 134, 200, 379, 777
AfiI CCNNNNNNNGG 5 cut(s) 69, 70, 381, 623, 790
AflII CTTAAG 1 cut(s) 730
AgsI TTSAA 2 cut(s) 324, 872
AhdI GACNNNNNGTC 1 cut(s) 112
AjnI CCWGG 2 cut(s) 829, 876
AluBI AGCT 9 cut(s) 10, 57, 122, 280, 364, 509, 518, 620, 848
AluI AGCT 9 cut(s) 10, 57, 122, 280, 364, 509, 518, 620, 848
Alw21I GWGCWC 1 cut(s) 282
Alw26I GTCTC 1 cut(s) 238
AlwI GGATC 4 cut(s) 228, 398, 745, 758
AlwNI CAGNNNCTG 1 cut(s) 512
AoxI GGCC 6 cut(s) 82, 770, 806, 826, 832, 855
ApeKI GCWGC 3 cut(s) 149, 611, 620
ApoI RAATTY 4 cut(s) 221, 319, 343, 867
AspS9I GGNCC 2 cut(s) 827, 856
AsuC2I CCSGG 1 cut(s) 705
AsuHPI GGTGA 8 cut(s) 17, 156, 239, 413, 647, 676, 709, 718
BamHI GGATCC 1 cut(s) 750
BanII GRGCYC 1 cut(s) 282
BauI CACGAG 1 cut(s) 254
BbsI GAAGAC 1 cut(s) 213
Bbv12I GWGCWC 1 cut(s) 282
BbvI GCAGC 3 cut(s) 161, 598, 607
BccI CCATC 1 cut(s) 818
BceAI ACGGC 2 cut(s) 97, 785
BciT130I CCWGG 2 cut(s) 831, 878
BcnI CCSGG 1 cut(s) 705
BcoDI GTCTC 1 cut(s) 238
BfrI CTTAAG 1 cut(s) 730
BglI GCCNNNNNGGC 1 cut(s) 840
BisI GCNGC 3 cut(s) 150, 612, 621
BlsI GCNGC 3 cut(s) 151, 613, 622
BmcAI AGTACT 1 cut(s) 379
Bme1390I CCNGG 3 cut(s) 705, 831, 878
BmeRI GACNNNNNGTC 1 cut(s) 112
BmgT120I GGNCC 2 cut(s) 827, 856
BmiI GGNNCC 1 cut(s) 752
BmrFI CCNGG 3 cut(s) 705, 831, 878
BmrI ACTGGG 3 cut(s) 337, 390, 779
BmuI ACTGGG 3 cut(s) 337, 390, 779
BpiI GAAGAC 1 cut(s) 213
BpmI CTGGAG 2 cut(s) 471, 600
BpuMI CCSGG 1 cut(s) 705
BsaHI GRCGYC 2 cut(s) 210, 723
BsaJI CCNNGG 8 cut(s) 27, 64, 88, 213, 444, 829, 876, 877
Bsc4I CCNNNNNNNGG 5 cut(s) 69, 70, 381, 623, 790
Bse118I RCCGGY 1 cut(s) 623
Bse1I ACTGG 6 cut(s) 320, 332, 375, 385, 773, 785
BseBI CCWGG 2 cut(s) 831, 878
BseDI CCNNGG 8 cut(s) 27, 64, 88, 213, 444, 829, 876, 877
BseGI GGATG 2 cut(s) 133, 829
BseLI CCNNNNNNNGG 5 cut(s) 69, 70, 381, 623, 790
BseMII CTCAG 1 cut(s) 330
BseNI ACTGG 6 cut(s) 320, 332, 375, 385, 773, 785
BseRI GAGGAG 2 cut(s) 461, 520
BseXI GCAGC 3 cut(s) 161, 598, 607
BshFI GGCC 6 cut(s) 84, 772, 808, 828, 834, 857
BsiHKAI GWGCWC 1 cut(s) 282
BsiSI CCGG 2 cut(s) 624, 705
BslFI GGGAC 1 cut(s) 806
BslI CCNNNNNNNGG 5 cut(s) 69, 70, 381, 623, 790
BsmAI GTCTC 1 cut(s) 238
BsmBI CGTCTC 1 cut(s) 238
BsmFI GGGAC 1 cut(s) 806
BsnI GGCC 6 cut(s) 84, 772, 808, 828, 834, 857
Bsp1286I GDGCHC 1 cut(s) 282
Bsp143I GATC 5 cut(s) 175, 233, 403, 450, 750
Bsp19I CCATGG 1 cut(s) 27
BspACI CCGC 2 cut(s) 708, 821
BspANI GGCC 6 cut(s) 84, 772, 808, 828, 834, 857
BspCNI CTCAG 1 cut(s) 331
BspLI GGNNCC 1 cut(s) 752
BspPI GGATC 4 cut(s) 228, 398, 745, 758
BspQI GCTCTTC 2 cut(s) 64, 486
BspTI CTTAAG 1 cut(s) 730
BsrFI RCCGGY 1 cut(s) 623
BsrI ACTGG 6 cut(s) 320, 332, 375, 385, 773, 785
BssAI RCCGGY 1 cut(s) 623
BssECI CCNNGG 8 cut(s) 27, 64, 88, 213, 444, 829, 876, 877
BssMI GATC 5 cut(s) 175, 233, 403, 450, 750
BssNAI GTATAC 1 cut(s) 261
BssNI GRCGYC 2 cut(s) 210, 723
BssSI CACGAG 1 cut(s) 254
BssT1I CCWWGG 4 cut(s) 27, 64, 88, 213
Bst1107I GTATAC 1 cut(s) 261
Bst2BI CACGAG 1 cut(s) 254
Bst2UI CCWGG 2 cut(s) 831, 878
Bst4CI ACNGT 1 cut(s) 44
Bst6I CTCTTC 5 cut(s) 64, 302, 486, 538, 653
BstACI GRCGYC 2 cut(s) 210, 723
BstAFI CTTAAG 1 cut(s) 730
BstC8I GCNNGC 2 cut(s) 251, 362
BstDEI CTNAG 2 cut(s) 202, 339
BstDSI CCRYGG 1 cut(s) 27
BstF5I GGATG 2 cut(s) 133, 829
BstKTI GATC 5 cut(s) 178, 236, 406, 453, 753
BstMAI GTCTC 1 cut(s) 238
BstMBI GATC 5 cut(s) 175, 233, 403, 450, 750
BstMWI GCNNNNNNNGC 6 cut(s) 54, 515, 617, 620, 840, 854
BstNI CCWGG 2 cut(s) 831, 878
BstNSI RCATGY 1 cut(s) 804
BstSCI CCNGG 3 cut(s) 703, 829, 876
BstV1I GCAGC 3 cut(s) 161, 598, 607
BstV2I GAAGAC 1 cut(s) 213
BstX2I RGATCY 1 cut(s) 750
BstXI CCANNNNNNTGG 1 cut(s) 28
BstYI RGATCY 1 cut(s) 750
BstZ17I GTATAC 1 cut(s) 261
BsuRI GGCC 6 cut(s) 84, 772, 808, 828, 834, 857
BtgI CCRYGG 1 cut(s) 27
BtsCI GGATG 2 cut(s) 133, 829
Cac8I GCNNGC 2 cut(s) 251, 362
CaiI CAGNNNCTG 1 cut(s) 512
Cfr10I RCCGGY 1 cut(s) 623
Cfr13I GGNCC 2 cut(s) 827, 856
CseI GACGC 2 cut(s) 218, 255
Csp6I GTAC 4 cut(s) 133, 199, 378, 776
CviAII CATG 7 cut(s) 22, 28, 136, 170, 355, 801, 810
CviQI GTAC 4 cut(s) 133, 199, 378, 776
DdeI CTNAG 2 cut(s) 202, 339
DpnI GATC 5 cut(s) 177, 235, 405, 452, 752
DpnII GATC 5 cut(s) 175, 233, 403, 450, 750
DriI GACNNNNNGTC 1 cut(s) 112
EaeI YGGCCR 3 cut(s) 82, 770, 806
Eam1104I CTCTTC 5 cut(s) 64, 302, 486, 538, 653
Eam1105I GACNNNNNGTC 1 cut(s) 112
EarI CTCTTC 5 cut(s) 64, 302, 486, 538, 653
EciI GGCGGA 1 cut(s) 723
Ecl136II GAGCTC 1 cut(s) 280
Eco130I CCWWGG 4 cut(s) 27, 64, 88, 213
Eco24I GRGCYC 1 cut(s) 282
Eco53kI GAGCTC 1 cut(s) 280
Eco57I CTGAAG 1 cut(s) 534
EcoICRI GAGCTC 1 cut(s) 280
EcoRI GAATTC 2 cut(s) 319, 867
EcoRII CCWGG 2 cut(s) 829, 876
EcoT14I CCWWGG 4 cut(s) 27, 64, 88, 213
EcoT38I GRGCYC 1 cut(s) 282
ErhI CCWWGG 4 cut(s) 27, 64, 88, 213
Esp3I CGTCTC 1 cut(s) 238
FaeI CATG 7 cut(s) 25, 31, 139, 173, 358, 804, 813
FalI AAGNNNNNCTT 2 cut(s) 579, 611
FaqI GGGAC 1 cut(s) 806
FatI CATG 7 cut(s) 21, 27, 135, 169, 354, 800, 809
FblI GTMKAC 2 cut(s) 260, 468
Fnu4HI GCNGC 3 cut(s) 150, 612, 621
FokI GGATG 2 cut(s) 140, 836
FriOI GRGCYC 1 cut(s) 282
Fsp4HI GCNGC 3 cut(s) 150, 612, 621
GluI GCNGC 3 cut(s) 150, 612, 621
GsuI CTGGAG 2 cut(s) 471, 600
HaeIII GGCC 6 cut(s) 84, 772, 808, 828, 834, 857
HapII CCGG 2 cut(s) 624, 705
HgaI GACGC 2 cut(s) 218, 255
Hin1I GRCGYC 2 cut(s) 210, 723
Hin1II CATG 7 cut(s) 25, 31, 139, 173, 358, 804, 813
HincII GTYRAC 1 cut(s) 469
HindII GTYRAC 1 cut(s) 469
HindIII AAGCTT 1 cut(s) 516
HinfI GANTC 5 cut(s) 287, 435, 470, 524, 548
HpaII CCGG 2 cut(s) 624, 705
HphI GGTGA 8 cut(s) 17, 156, 239, 413, 647, 676, 709, 718
Hpy166II GTNNAC 2 cut(s) 261, 469
Hpy188I TCNGA 5 cut(s) 184, 340, 445, 568, 905
Hpy188III TCNNGA 3 cut(s) 190, 488, 512
Hpy8I GTNNAC 2 cut(s) 261, 469
Hpy99I CGWCG 3 cut(s) 196, 470, 725
HpyAV CCTTC 1 cut(s) 710
HpyCH4III ACNGT 1 cut(s) 44
HpyCH4IV ACGT 1 cut(s) 723
HpyCH4V TGCA 3 cut(s) 354, 543, 800
HpyF10VI GCNNNNNNNGC 6 cut(s) 54, 515, 617, 620, 840, 854
HpyF3I CTNAG 2 cut(s) 202, 339
HpySE526I ACGT 1 cut(s) 723
Hsp92I GRCGYC 2 cut(s) 210, 723
Hsp92II CATG 7 cut(s) 25, 31, 139, 173, 358, 804, 813
Kzo9I GATC 5 cut(s) 175, 233, 403, 450, 750
LguI GCTCTTC 2 cut(s) 64, 486
LmnI GCTCC 2 cut(s) 514, 619
Lsp1109I GCAGC 3 cut(s) 161, 598, 607
MaeII ACGT 1 cut(s) 723
MaeIII GTNAC 2 cut(s) 77, 724
MalI GATC 5 cut(s) 177, 235, 405, 452, 752
MboI GATC 5 cut(s) 175, 233, 403, 450, 750
MboII GAAGA 8 cut(s) 51, 218, 319, 503, 525, 528, 667, 670
MflI RGATCY 1 cut(s) 750
MhlI GDGCHC 1 cut(s) 282
MluCI AATT 5 cut(s) 221, 319, 332, 343, 867
MlyI GAGTC 3 cut(s) 281, 429, 464
MnlI CCTC 8 cut(s) 59, 177, 439, 538, 541, 654, 741, 878
MseI TTAA 2 cut(s) 731, 916
MslI CAYNNNNRTG 2 cut(s) 26, 140
MspA1I CMGCKG 1 cut(s) 620
MspCI CTTAAG 1 cut(s) 730
MspI CCGG 2 cut(s) 624, 705
MspR9I CCNGG 3 cut(s) 705, 831, 878
MvaI CCWGG 2 cut(s) 831, 878
MwoI GCNNNNNNNGC 6 cut(s) 54, 515, 617, 620, 840, 854
NciI CCSGG 1 cut(s) 705
NcoI CCATGG 1 cut(s) 27
NdeII GATC 5 cut(s) 175, 233, 403, 450, 750
NlaIII CATG 7 cut(s) 25, 31, 139, 173, 358, 804, 813
NlaIV GGNNCC 1 cut(s) 752
NmuCI GTSAC 2 cut(s) 77, 724
NspI RCATGY 1 cut(s) 804
PasI CCCWGGG 1 cut(s) 877
PciSI GCTCTTC 2 cut(s) 64, 486
PcsI WCGNNNNNNNCGW 1 cut(s) 775
PfeI GAWTC 2 cut(s) 524, 548
PflFI GACNNNGTC 1 cut(s) 464
PkrI GCNGC 3 cut(s) 151, 613, 622
PleI GAGTC 3 cut(s) 281, 429, 464
PpsI GAGTC 3 cut(s) 281, 429, 464
Psp124BI GAGCTC 1 cut(s) 282
Psp6I CCWGG 2 cut(s) 829, 876
PspGI CCWGG 2 cut(s) 829, 876
PspN4I GGNNCC 1 cut(s) 752
PspPI GGNCC 2 cut(s) 827, 856
PstNI CAGNNNCTG 1 cut(s) 512
PsuI RGATCY 1 cut(s) 750
PsyI GACNNNGTC 1 cut(s) 464
PvuII CAGCTG 1 cut(s) 620
RsaI GTAC 4 cut(s) 134, 200, 379, 777
RsaNI GTAC 4 cut(s) 133, 199, 378, 776
RseI CAYNNNNRTG 2 cut(s) 26, 140
SacI GAGCTC 1 cut(s) 282
SalI GTCGAC 1 cut(s) 467
SapI GCTCTTC 2 cut(s) 64, 486
SaqAI TTAA 2 cut(s) 731, 916
SatI GCNGC 3 cut(s) 150, 612, 621
Sau3AI GATC 5 cut(s) 175, 233, 403, 450, 750
Sau96I GGNCC 2 cut(s) 827, 856
ScaI AGTACT 1 cut(s) 379
SchI GAGTC 3 cut(s) 281, 429, 464
ScrFI CCNGG 3 cut(s) 705, 831, 878
SduI GDGCHC 1 cut(s) 282
SmiMI CAYNNNNRTG 2 cut(s) 26, 140
SmlI CTYRAG 1 cut(s) 730
SmoI CTYRAG 1 cut(s) 730
Sse9I AATT 5 cut(s) 221, 319, 332, 343, 867
SsiI CCGC 2 cut(s) 708, 821
SspI AATATT 1 cut(s) 607
SstI GAGCTC 1 cut(s) 282
StyD4I CCNGG 3 cut(s) 703, 829, 876
StyI CCWWGG 4 cut(s) 27, 64, 88, 213
TaaI ACNGT 1 cut(s) 44
TaiI ACGT 1 cut(s) 726
TaqI TCGA 3 cut(s) 191, 468, 551
TasI AATT 5 cut(s) 221, 319, 332, 343, 867
TatI WGTACW 2 cut(s) 132, 377
TfiI GAWTC 2 cut(s) 524, 548
Tru1I TTAA 2 cut(s) 731, 916
Tru9I TTAA 2 cut(s) 731, 916
TseFI GTSAC 2 cut(s) 77, 724
TseI GCWGC 3 cut(s) 149, 611, 620
Tsp45I GTSAC 2 cut(s) 77, 724
TspDTI ATGAA 3 cut(s) 158, 443, 654
Tth111I GACNNNGTC 1 cut(s) 464
Vha464I CTTAAG 1 cut(s) 730
XapI RAATTY 4 cut(s) 221, 319, 343, 867
XceI RCATGY 1 cut(s) 804
XmiI GTMKAC 2 cut(s) 260, 468
ZraI GACGTC 1 cut(s) 724
ZrmI AGTACT 1 cut(s) 379
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.