MD16G1011100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0009989
Physical Location & Seq
Forward (+)
840380 .. 842111
1732 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1011100.v1.1.491

Sequence Viewer

Length: 921 bp
ATGGGCGAGCTTCATCACTCCATGTCCATGGCTTTCACAAACAGTAGCCTTCACAGCTCTTCCCTAGGTTGGGATTGTCACAGCCACCTTGGAGTCCTCAACACAGACAGAATGTCTTTAGCTATGGAAGAGTACATGATGATGAATGTAGCATTTTACTCATCGCCTCATGTAGATCAGTCGGATTTCTCGACGGGGTACTTAGAAGACGCTTTGGTTGAATTTAGCGAGCGATCCAAAAGAAGACGCTTGCTCGCGTATACCGATGGTGATAACGAGCTCAAAGACTCCTCCACTGCTCTTCTTGCGAAGGGGTACTGGAATTCAGACTGGGAAGTGTCTGAGAATTTTGATTGCATGAGCCAGCTAACTACTTCCAGTTCTGGGCCATCAGTTCTTCTAGGTGATCCTGTAAGTATAACCACACCAATGAGCAGACTCGTGTCCGAAGAGATCACAAACGACATAGTCGGCACAAAGATAAACACACCAGAAGAGCCAACAATGTCAGCTCCCGAAGCTTTTGATTCCTCCTCTTCTTCTTCTAGCAAAGACGCGGCCAACACCAACTCTGATTATTTACCTGCTCCCAAAGAAACTTTTTTAACCAATATTGCTGTTCCAGCTGTAGGTGGTGGTGGTGATGAGAAGAAAAAAAAGAAGAGGGTGATAACAAGGGTGGTCTATCCATTTGCGTTGGTGAAGCCGGGCGGAGTAGAGGGCGACGTGACCTTGAACGACATCAATGAGAGAATCCTAATGCCGCCAACACGGCCAGTACGACACCCAGTTGGGGACTTTGCGTGTCGACCATGCGTGTCTGCCGATGGCCCTGGCCTTTCTGGCAAAGCTGTGGTGGCCCTTACCAGGATTCAAACCCAGGGCAGAGGCACAATCACTATTATCAGAACCAAAGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

307

Amino Acids

33.15

Weight (kDa)

5.31

Isoelectric Point (pI)

57.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009989)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14630 AT1G14630 AT2G01990 AT2G01990
fragaria_vesca FvH4_4g35920
malus_domestica MD13G1013900.v1.1 MD16G1011100.v1.1
prunus_persica Prupe.1G339600_v2.0.a1 Prupe.1G339600_v2.0.a1
pyrus_communis pycom13g01180 pycom16g01090
rosa_chinensis RchiOBHm_Chr4g0445521
rosa_laevigata RLG00000005733
rosa_multiflora Rmu_ssc0000253.1_g000002
rosa_roxburghii Rroxscaffold_5G00386000
rosa_rugosa Rorug04G0362100
rosa_samantha Rh4AG423600 Rh4BG435600 Rh4CG450300 Rh4DG431900
rosa_wichuraiana Rw4G036310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 592
AccI GTMKAC 2 cut(s) 260, 808
AccII CGCG 2 cut(s) 257, 557
AciI CCGC 3 cut(s) 557, 711, 764
AclWI GGATC 2 cut(s) 228, 401
AcoI YGGCCR 2 cut(s) 558, 773
AcsI RAATTY 3 cut(s) 221, 322, 346
AfaI GTAC 4 cut(s) 134, 200, 317, 780
AfiI CCNNNNNNNGG 6 cut(s) 69, 70, 384, 629, 793, 867
AgsI TTSAA 3 cut(s) 221, 736, 875
AhdI GACNNNNNGTC 1 cut(s) 112
AjiI CACGTC 1 cut(s) 727
AjnI CCWGG 3 cut(s) 832, 866, 879
AluBI AGCT 9 cut(s) 10, 57, 122, 280, 367, 512, 521, 626, 851
AluI AGCT 9 cut(s) 10, 57, 122, 280, 367, 512, 521, 626, 851
Alw21I GWGCWC 1 cut(s) 282
AlwI GGATC 2 cut(s) 228, 401
AoxI GGCC 6 cut(s) 386, 558, 773, 829, 835, 858
ApoI RAATTY 3 cut(s) 221, 322, 346
AspA2I CCTAGG 1 cut(s) 64
AspS9I GGNCC 3 cut(s) 386, 830, 859
AsuC2I CCSGG 1 cut(s) 708
AsuHPI GGTGA 5 cut(s) 281, 416, 653, 679, 712
AvrII CCTAGG 1 cut(s) 64
BanII GRGCYC 1 cut(s) 282
BauI CACGAG 1 cut(s) 440
BbsI GAAGAC 2 cut(s) 213, 250
Bbv12I GWGCWC 1 cut(s) 282
BccI CCATC 3 cut(s) 260, 397, 821
BceAI ACGGC 1 cut(s) 788
BciT130I CCWGG 3 cut(s) 834, 868, 881
BcnI CCSGG 1 cut(s) 708
BfaI CTAG 3 cut(s) 65, 401, 546
BfmI CTRYAG 1 cut(s) 627
BfuAI ACCTGC 1 cut(s) 592
BglI GCCNNNNNGGC 2 cut(s) 772, 843
BisI GCNGC 2 cut(s) 558, 764
BlnI CCTAGG 1 cut(s) 64
BlsI GCNGC 2 cut(s) 559, 765
Bme1390I CCNGG 4 cut(s) 708, 834, 868, 881
BmeRI GACNNNNNGTC 1 cut(s) 112
BmgBI CACGTC 1 cut(s) 727
BmgT120I GGNCC 3 cut(s) 386, 830, 859
BmrFI CCNGG 4 cut(s) 708, 834, 868, 881
BmrI ACTGGG 2 cut(s) 340, 782
BmuI ACTGGG 2 cut(s) 340, 782
BoxI GACNNNNGTC 1 cut(s) 442
BpiI GAAGAC 2 cut(s) 213, 250
BpuMI CCSGG 1 cut(s) 708
BsaJI CCNNGG 6 cut(s) 27, 64, 88, 832, 879, 880
Bsc4I CCNNNNNNNGG 6 cut(s) 69, 70, 384, 629, 793, 867
Bse1I ACTGG 5 cut(s) 323, 335, 378, 776, 788
BseBI CCWGG 3 cut(s) 834, 868, 881
BseDI CCNNGG 6 cut(s) 27, 64, 88, 832, 879, 880
BseLI CCNNNNNNNGG 6 cut(s) 69, 70, 384, 629, 793, 867
BseMII CTCAG 1 cut(s) 333
BseNI ACTGG 5 cut(s) 323, 335, 378, 776, 788
BseRI GAGGAG 2 cut(s) 280, 523
Bsh1236I CGCG 2 cut(s) 257, 557
BshFI GGCC 6 cut(s) 388, 560, 775, 831, 837, 860
BsiHKAI GWGCWC 1 cut(s) 282
BsiSI CCGG 1 cut(s) 707
BslFI GGGAC 1 cut(s) 809
BslI CCNNNNNNNGG 6 cut(s) 69, 70, 384, 629, 793, 867
BsmFI GGGAC 1 cut(s) 809
BsnI GGCC 6 cut(s) 388, 560, 775, 831, 837, 860
Bsp1286I GDGCHC 1 cut(s) 282
Bsp143I GATC 4 cut(s) 175, 233, 406, 453
Bsp19I CCATGG 1 cut(s) 27
BspACI CCGC 3 cut(s) 557, 711, 764
BspANI GGCC 6 cut(s) 388, 560, 775, 831, 837, 860
BspCNI CTCAG 1 cut(s) 334
BspFNI CGCG 2 cut(s) 257, 557
BspMI ACCTGC 1 cut(s) 592
BspPI GGATC 2 cut(s) 228, 401
BspQI GCTCTTC 3 cut(s) 64, 306, 489
BsrI ACTGG 5 cut(s) 323, 335, 378, 776, 788
BssECI CCNNGG 6 cut(s) 27, 64, 88, 832, 879, 880
BssMI GATC 4 cut(s) 175, 233, 406, 453
BssNAI GTATAC 1 cut(s) 261
BssSI CACGAG 1 cut(s) 440
BssT1I CCWWGG 3 cut(s) 27, 64, 88
Bst1107I GTATAC 1 cut(s) 261
Bst2BI CACGAG 1 cut(s) 440
Bst2UI CCWGG 3 cut(s) 834, 868, 881
Bst4CI ACNGT 1 cut(s) 44
Bst6I CTCTTC 7 cut(s) 64, 123, 306, 444, 489, 541, 656
BstC8I GCNNGC 5 cut(s) 8, 230, 251, 255, 365
BstDEI CTNAG 2 cut(s) 202, 342
BstDSI CCRYGG 1 cut(s) 27
BstFNI CGCG 2 cut(s) 257, 557
BstKTI GATC 4 cut(s) 178, 236, 409, 456
BstMBI GATC 4 cut(s) 175, 233, 406, 453
BstMWI GCNNNNNNNGC 7 cut(s) 54, 305, 518, 623, 772, 843, 857
BstNI CCWGG 3 cut(s) 834, 868, 881
BstPAI GACNNNNGTC 1 cut(s) 442
BstSCI CCNGG 4 cut(s) 706, 832, 866, 879
BstSFI CTRYAG 1 cut(s) 627
BstUI CGCG 2 cut(s) 257, 557
BstV2I GAAGAC 2 cut(s) 213, 250
BstXI CCANNNNNNTGG 1 cut(s) 28
BstZ17I GTATAC 1 cut(s) 261
BsuRI GGCC 6 cut(s) 388, 560, 775, 831, 837, 860
BtgI CCRYGG 1 cut(s) 27
BtgZI GCGATG 1 cut(s) 147
BtrI CACGTC 1 cut(s) 727
BtsI GCAGTG 1 cut(s) 294
BtsIMutI CAGTG 1 cut(s) 294
BveI ACCTGC 1 cut(s) 592
Cac8I GCNNGC 5 cut(s) 8, 230, 251, 255, 365
Cfr13I GGNCC 3 cut(s) 386, 830, 859
CseI GACGC 3 cut(s) 218, 255, 563
Csp6I GTAC 4 cut(s) 133, 199, 316, 779
CviAII CATG 6 cut(s) 22, 28, 136, 170, 358, 813
CviQI GTAC 4 cut(s) 133, 199, 316, 779
DdeI CTNAG 2 cut(s) 202, 342
DpnI GATC 4 cut(s) 177, 235, 408, 455
DpnII GATC 4 cut(s) 175, 233, 406, 453
DriI GACNNNNNGTC 1 cut(s) 112
EaeI YGGCCR 2 cut(s) 558, 773
Eam1104I CTCTTC 7 cut(s) 64, 123, 306, 444, 489, 541, 656
Eam1105I GACNNNNNGTC 1 cut(s) 112
EarI CTCTTC 7 cut(s) 64, 123, 306, 444, 489, 541, 656
EciI GGCGGA 1 cut(s) 726
Ecl136II GAGCTC 1 cut(s) 280
Eco130I CCWWGG 3 cut(s) 27, 64, 88
Eco24I GRGCYC 1 cut(s) 282
Eco53kI GAGCTC 1 cut(s) 280
EcoICRI GAGCTC 1 cut(s) 280
EcoRI GAATTC 1 cut(s) 322
EcoRII CCWGG 3 cut(s) 832, 866, 879
EcoT14I CCWWGG 3 cut(s) 27, 64, 88
EcoT38I GRGCYC 1 cut(s) 282
ErhI CCWWGG 3 cut(s) 27, 64, 88
FaeI CATG 6 cut(s) 25, 31, 139, 173, 361, 816
FaqI GGGAC 1 cut(s) 809
FatI CATG 6 cut(s) 21, 27, 135, 169, 357, 812
FblI GTMKAC 2 cut(s) 260, 808
Fnu4HI GCNGC 2 cut(s) 558, 764
FriOI GRGCYC 1 cut(s) 282
Fsp4HI GCNGC 2 cut(s) 558, 764
FspBI CTAG 3 cut(s) 65, 401, 546
GluI GCNGC 2 cut(s) 558, 764
HaeIII GGCC 6 cut(s) 388, 560, 775, 831, 837, 860
HapII CCGG 1 cut(s) 707
HgaI GACGC 3 cut(s) 218, 255, 563
Hin1II CATG 6 cut(s) 25, 31, 139, 173, 361, 816
HincII GTYRAC 1 cut(s) 809
HindII GTYRAC 1 cut(s) 809
HindIII AAGCTT 1 cut(s) 519
HinfI GANTC 6 cut(s) 93, 287, 438, 527, 753, 871
HpaII CCGG 1 cut(s) 707
HphI GGTGA 5 cut(s) 281, 416, 653, 679, 712
Hpy166II GTNNAC 2 cut(s) 261, 809
Hpy188I TCNGA 6 cut(s) 184, 328, 343, 448, 574, 908
Hpy188III TCNNGA 2 cut(s) 190, 515
Hpy8I GTNNAC 2 cut(s) 261, 809
Hpy99I CGWCG 2 cut(s) 196, 728
HpyAV CCTTC 2 cut(s) 59, 304
HpyCH4III ACNGT 1 cut(s) 44
HpyCH4IV ACGT 1 cut(s) 726
HpyCH4V TGCA 1 cut(s) 357
HpyF10VI GCNNNNNNNGC 7 cut(s) 54, 305, 518, 623, 772, 843, 857
HpyF3I CTNAG 2 cut(s) 202, 342
HpySE526I ACGT 1 cut(s) 726
Hsp92II CATG 6 cut(s) 25, 31, 139, 173, 361, 816
Kzo9I GATC 4 cut(s) 175, 233, 406, 453
LguI GCTCTTC 3 cut(s) 64, 306, 489
LmnI GCTCC 2 cut(s) 517, 592
MaeI CTAG 3 cut(s) 65, 401, 546
MaeII ACGT 1 cut(s) 726
MaeIII GTNAC 2 cut(s) 77, 727
MalI GATC 4 cut(s) 177, 235, 408, 455
MboI GATC 4 cut(s) 175, 233, 406, 453
MhlI GDGCHC 1 cut(s) 282
MluCI AATT 3 cut(s) 221, 322, 346
MlyI GAGTC 3 cut(s) 102, 281, 432
MmeI TCCRAC 1 cut(s) 162
MnlI CCTC 8 cut(s) 107, 177, 301, 541, 544, 657, 712, 881
MseI TTAA 2 cut(s) 605, 919
MslI CAYNNNNRTG 3 cut(s) 26, 140, 428
MspA1I CMGCKG 1 cut(s) 626
MspI CCGG 1 cut(s) 707
MspR9I CCNGG 4 cut(s) 708, 834, 868, 881
MvaI CCWGG 3 cut(s) 834, 868, 881
MvnI CGCG 2 cut(s) 257, 557
MwoI GCNNNNNNNGC 7 cut(s) 54, 305, 518, 623, 772, 843, 857
NciI CCSGG 1 cut(s) 708
NcoI CCATGG 1 cut(s) 27
NdeII GATC 4 cut(s) 175, 233, 406, 453
NlaIII CATG 6 cut(s) 25, 31, 139, 173, 361, 816
NmuCI GTSAC 2 cut(s) 77, 727
PasI CCCWGGG 1 cut(s) 880
PciSI GCTCTTC 3 cut(s) 64, 306, 489
PcsI WCGNNNNNNNCGW 3 cut(s) 188, 261, 778
PfeI GAWTC 3 cut(s) 527, 753, 871
PflFI GACNNNGTC 1 cut(s) 467
PkrI GCNGC 2 cut(s) 559, 765
PleI GAGTC 3 cut(s) 101, 281, 432
PpsI GAGTC 3 cut(s) 101, 281, 432
PshAI GACNNNNGTC 1 cut(s) 442
Psp124BI GAGCTC 1 cut(s) 282
Psp6I CCWGG 3 cut(s) 832, 866, 879
PspGI CCWGG 3 cut(s) 832, 866, 879
PspPI GGNCC 3 cut(s) 386, 830, 859
PsyI GACNNNGTC 1 cut(s) 467
PvuII CAGCTG 1 cut(s) 626
RsaI GTAC 4 cut(s) 134, 200, 317, 780
RsaNI GTAC 4 cut(s) 133, 199, 316, 779
RseI CAYNNNNRTG 3 cut(s) 26, 140, 428
SacI GAGCTC 1 cut(s) 282
SalI GTCGAC 1 cut(s) 807
SapI GCTCTTC 3 cut(s) 64, 306, 489
SaqAI TTAA 2 cut(s) 605, 919
SatI GCNGC 2 cut(s) 558, 764
Sau3AI GATC 4 cut(s) 175, 233, 406, 453
Sau96I GGNCC 3 cut(s) 386, 830, 859
SchI GAGTC 3 cut(s) 102, 281, 432
ScrFI CCNGG 4 cut(s) 708, 834, 868, 881
SduI GDGCHC 1 cut(s) 282
SfcI CTRYAG 1 cut(s) 627
SmiMI CAYNNNNRTG 3 cut(s) 26, 140, 428
Sse9I AATT 3 cut(s) 221, 322, 346
SsiI CCGC 3 cut(s) 557, 711, 764
SspI AATATT 1 cut(s) 613
SspMI CTAG 3 cut(s) 65, 401, 546
SstI GAGCTC 1 cut(s) 282
StyD4I CCNGG 4 cut(s) 706, 832, 866, 879
StyI CCWWGG 3 cut(s) 27, 64, 88
TaaI ACNGT 1 cut(s) 44
TaiI ACGT 1 cut(s) 729
TaqI TCGA 2 cut(s) 191, 808
TasI AATT 3 cut(s) 221, 322, 346
TatI WGTACW 1 cut(s) 132
TauI GCSGC 2 cut(s) 560, 766
TfiI GAWTC 3 cut(s) 527, 753, 871
Tru1I TTAA 2 cut(s) 605, 919
Tru9I TTAA 2 cut(s) 605, 919
TscAI CASTG 1 cut(s) 301
TseFI GTSAC 2 cut(s) 77, 727
Tsp45I GTSAC 2 cut(s) 77, 727
TspDTI ATGAA 1 cut(s) 158
TspRI CASTG 1 cut(s) 301
Tth111I GACNNNGTC 1 cut(s) 467
XapI RAATTY 3 cut(s) 221, 322, 346
XmaJI CCTAGG 1 cut(s) 64
XmiI GTMKAC 2 cut(s) 260, 808
XspI CTAG 3 cut(s) 65, 401, 546
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.