Rh4DG431900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0009989
Physical Location & Seq
Reverse (-)
63611021 .. 63613856
2836 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG431900.1

Sequence Viewer

Length: 738 bp
ATGGATGACTTTAATGCCGCACCACCATTTTCTTCTACTCTCGAGTCGGATTTCTCGACCGGTTACTTGGAAGACGCTTTGGTAGAATTCAGTGAGCGCTCGAAAAGAAGACGCATGCTGTTGTTTAACGATGACGAAATCAGAGACTCCACCGCTGTTGCTTCAAAGAGACACTGGAATTCAGACTGGGAAGTGCTTGAGAGTTTTGACAGCATTAGCCAACTAATCACCTCTACTGGGCTATCAGGGGATGATCCTACAAGTATGAGTACTACACCAGTGAGCAGAATCTGTGAGGATGAGACCAACATAGCCGATACAAAGATGAACGCAAGAGAAGAAGCTATTGATTCCTCGTCTTCATCTTCTTCTTGCAAAGAATCAGCCAACACCAACTCCATGGCTGCCGAGAAAGAGAACCTCTTTTCCGCTAATGCTCCTCTGGCTGCAGATGAGAAGAGAAATAAGAAGAGGCCGGTGATAACGTCGAGGGTGGTGTATCCCTTTGCGGTTGTGAAACCGGGAGGAGTAGAAGGTGACGTGACGCTAAACGACATCAACGAGAGGATTTCAATGCCACCAACGCGACCAGTACGACACCCCGTTGGGGACTTTGCGTGTCGGCCATGCGTATCTGATCCTGATGGTCCAGGACTTTCTGGTAAAGCTGTGGTGGCCCTTACCAGAATTCAAACACAGGGAAGAGGCACAATCACTATTATCAGAACCAAAGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

245

Amino Acids

26.68

Weight (kDa)

4.96

Isoelectric Point (pI)

55.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009989)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14630 AT1G14630 AT2G01990 AT2G01990
fragaria_vesca FvH4_4g35920
malus_domestica MD13G1013900.v1.1 MD16G1011100.v1.1
prunus_persica Prupe.1G339600_v2.0.a1 Prupe.1G339600_v2.0.a1
pyrus_communis pycom13g01180 pycom16g01090
rosa_chinensis RchiOBHm_Chr4g0445521
rosa_laevigata RLG00000005733
rosa_multiflora Rmu_ssc0000253.1_g000002
rosa_roxburghii Rroxscaffold_5G00386000
rosa_rugosa Rorug04G0362100
rosa_samantha Rh4AG423600 Rh4BG435600 Rh4CG450300 Rh4DG431900
rosa_wichuraiana Rw4G036310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 586
AciI CCGC 4 cut(s) 18, 153, 429, 509
AclWI GGATC 2 cut(s) 248, 632
AcoI YGGCCR 1 cut(s) 623
AcsI RAATTY 3 cut(s) 86, 178, 687
AfaI GTAC 2 cut(s) 271, 594
AfeI AGCGCT 1 cut(s) 98
AfiI CCNNNNNNNGG 2 cut(s) 237, 607
AgeI ACCGGT 1 cut(s) 59
AgsI TTSAA 3 cut(s) 165, 573, 692
AjiI CACGTC 1 cut(s) 541
AjnI CCWGG 1 cut(s) 649
AloI GAACNNNNNNTCC 2 cut(s) 410, 442
AluBI AGCT 2 cut(s) 344, 668
AluI AGCT 2 cut(s) 344, 668
Alw26I GTCTC 3 cut(s) 138, 163, 296
AlwI GGATC 2 cut(s) 248, 632
AlwNI CAGNNNCTG 1 cut(s) 291
Ama87I CYCGRG 1 cut(s) 41
Aor51HI AGCGCT 1 cut(s) 98
AoxI GGCC 3 cut(s) 473, 623, 675
ApeKI GCWGC 2 cut(s) 404, 446
ApoI RAATTY 3 cut(s) 86, 178, 687
AsiGI ACCGGT 1 cut(s) 59
AspLEI GCGC 1 cut(s) 99
AspS9I GGNCC 2 cut(s) 647, 676
AsuC2I CCSGG 1 cut(s) 522
AsuHPI GGTGA 3 cut(s) 220, 490, 548
AvaI CYCGRG 1 cut(s) 41
AvaII GGWCC 1 cut(s) 647
BbsI GAAGAC 3 cut(s) 78, 115, 351
BbvI GCAGC 2 cut(s) 391, 433
BccI CCATC 1 cut(s) 638
BciT130I CCWGG 1 cut(s) 651
BciVI GTATCC 1 cut(s) 510
BcnI CCSGG 1 cut(s) 522
BcoDI GTCTC 3 cut(s) 138, 163, 296
BfmI CTRYAG 1 cut(s) 447
BfoI RGCGCY 1 cut(s) 100
BfuI GTATCC 1 cut(s) 510
BisI GCNGC 3 cut(s) 18, 405, 447
BlsI GCNGC 3 cut(s) 19, 406, 448
BmcAI AGTACT 1 cut(s) 271
Bme1390I CCNGG 2 cut(s) 522, 651
Bme18I GGWCC 1 cut(s) 647
BmeT110I CYCGRG 1 cut(s) 41
BmgBI CACGTC 1 cut(s) 541
BmgT120I GGNCC 2 cut(s) 647, 676
BmrFI CCNGG 2 cut(s) 522, 651
BmrI ACTGGG 2 cut(s) 196, 246
BmuI ACTGGG 2 cut(s) 196, 246
BpiI GAAGAC 3 cut(s) 78, 115, 351
BpuEI CTTGAG 1 cut(s) 218
BpuMI CCSGG 1 cut(s) 522
BsaI GGTCTC 1 cut(s) 296
BsaJI CCNNGG 1 cut(s) 399
BsaWI WCCGGW 1 cut(s) 59
BsaXI ACNNNNNCTCC 2 cut(s) 380, 410
Bsc4I CCNNNNNNNGG 2 cut(s) 237, 607
Bse118I RCCGGY 2 cut(s) 59, 475
Bse1I ACTGG 5 cut(s) 179, 191, 241, 278, 590
BseBI CCWGG 1 cut(s) 651
BseDI CCNNGG 1 cut(s) 399
BseGI GGATG 3 cut(s) 10, 256, 304
BseLI CCNNNNNNNGG 2 cut(s) 237, 607
BseNI ACTGG 5 cut(s) 179, 191, 241, 278, 590
BseRI GAGGAG 2 cut(s) 429, 540
BseXI GCAGC 2 cut(s) 391, 433
Bsh1236I CGCG 1 cut(s) 586
Bsh1285I CGRYCG 1 cut(s) 60
BshFI GGCC 3 cut(s) 475, 625, 677
BshTI ACCGGT 1 cut(s) 59
BsiEI CGRYCG 1 cut(s) 60
BsiHKCI CYCGRG 1 cut(s) 41
BsiSI CCGG 3 cut(s) 60, 476, 521
BslFI GGGAC 1 cut(s) 623
BslI CCNNNNNNNGG 2 cut(s) 237, 607
BsmAI GTCTC 3 cut(s) 138, 163, 296
BsmFI GGGAC 1 cut(s) 623
BsnI GGCC 3 cut(s) 475, 625, 677
Bso31I GGTCTC 1 cut(s) 296
BsoBI CYCGRG 1 cut(s) 41
Bsp143I GATC 2 cut(s) 253, 637
Bsp19I CCATGG 1 cut(s) 399
BspACI CCGC 4 cut(s) 18, 153, 429, 509
BspANI GGCC 3 cut(s) 475, 625, 677
BspFNI CGCG 1 cut(s) 586
BspMAI CTGCAG 1 cut(s) 451
BspPI GGATC 2 cut(s) 248, 632
BspTNI GGTCTC 1 cut(s) 296
BsrFI RCCGGY 2 cut(s) 59, 475
BsrI ACTGG 5 cut(s) 179, 191, 241, 278, 590
BssAI RCCGGY 2 cut(s) 59, 475
BssECI CCNNGG 1 cut(s) 399
BssMI GATC 2 cut(s) 253, 637
BssT1I CCWWGG 1 cut(s) 399
Bst2UI CCWGG 1 cut(s) 651
Bst6I CTCTTC 3 cut(s) 452, 464, 697
BstC8I GCNNGC 1 cut(s) 116
BstDSI CCRYGG 1 cut(s) 399
BstF5I GGATG 3 cut(s) 10, 256, 304
BstFNI CGCG 1 cut(s) 586
BstH2I RGCGCY 1 cut(s) 100
BstHHI GCGC 1 cut(s) 99
BstKTI GATC 2 cut(s) 256, 640
BstMAI GTCTC 3 cut(s) 138, 163, 296
BstMBI GATC 2 cut(s) 253, 637
BstMCI CGRYCG 1 cut(s) 60
BstMWI GCNNNNNNNGC 3 cut(s) 443, 583, 674
BstNI CCWGG 1 cut(s) 651
BstNSI RCATGY 1 cut(s) 118
BstSCI CCNGG 2 cut(s) 520, 649
BstSFI CTRYAG 1 cut(s) 447
BstUI CGCG 1 cut(s) 586
BstV1I GCAGC 2 cut(s) 391, 433
BstV2I GAAGAC 3 cut(s) 78, 115, 351
BstXI CCANNNNNNTGG 1 cut(s) 400
BsuI GTATCC 1 cut(s) 510
BsuRI GGCC 3 cut(s) 475, 625, 677
BtgI CCRYGG 1 cut(s) 399
BtrI CACGTC 1 cut(s) 541
BtsCI GGATG 3 cut(s) 10, 256, 304
BtsIMutI CAGTG 3 cut(s) 97, 172, 285
Cac8I GCNNGC 1 cut(s) 116
CaiI CAGNNNCTG 1 cut(s) 291
CfoI GCGC 1 cut(s) 99
Cfr10I RCCGGY 2 cut(s) 59, 475
Cfr13I GGNCC 2 cut(s) 647, 676
CseI GACGC 3 cut(s) 83, 120, 553
Csp6I GTAC 2 cut(s) 270, 593
CspAI ACCGGT 1 cut(s) 59
CspCI CAANNNNNGTGG 2 cut(s) 139, 174
CviAII CATG 3 cut(s) 115, 400, 627
CviQI GTAC 2 cut(s) 270, 593
DpnI GATC 2 cut(s) 255, 639
DpnII GATC 2 cut(s) 253, 637
EaeI YGGCCR 1 cut(s) 623
Eam1104I CTCTTC 3 cut(s) 452, 464, 697
EarI CTCTTC 3 cut(s) 452, 464, 697
Eco130I CCWWGG 1 cut(s) 399
Eco31I GGTCTC 1 cut(s) 296
Eco47I GGWCC 1 cut(s) 647
Eco47III AGCGCT 1 cut(s) 98
Eco88I CYCGRG 1 cut(s) 41
EcoRI GAATTC 3 cut(s) 86, 178, 687
EcoRII CCWGG 1 cut(s) 649
EcoT14I CCWWGG 1 cut(s) 399
ErhI CCWWGG 1 cut(s) 399
FaeI CATG 3 cut(s) 118, 403, 630
FaiI YATR 5 cut(s) 116, 266, 311, 401, 628
FaqI GGGAC 1 cut(s) 623
FatI CATG 3 cut(s) 114, 399, 626
Fnu4HI GCNGC 3 cut(s) 18, 405, 447
FokI GGATG 3 cut(s) 17, 263, 311
Fsp4HI GCNGC 3 cut(s) 18, 405, 447
GlaI GCGC 1 cut(s) 98
GluI GCNGC 3 cut(s) 18, 405, 447
HaeII RGCGCY 1 cut(s) 100
HaeIII GGCC 3 cut(s) 475, 625, 677
HapII CCGG 3 cut(s) 60, 476, 521
HgaI GACGC 3 cut(s) 83, 120, 553
HhaI GCGC 1 cut(s) 99
Hin1II CATG 3 cut(s) 118, 403, 630
Hin6I GCGC 1 cut(s) 97
HinP1I GCGC 1 cut(s) 97
HinfI GANTC 5 cut(s) 44, 146, 288, 350, 380
HpaII CCGG 3 cut(s) 60, 476, 521
HphI GGTGA 3 cut(s) 220, 490, 548
Hpy188I TCNGA 5 cut(s) 49, 143, 184, 637, 725
Hpy188III TCNNGA 3 cut(s) 41, 55, 641
Hpy99I CGWCG 1 cut(s) 490
HpyAV CCTTC 1 cut(s) 527
HpyCH4IV ACGT 2 cut(s) 485, 540
HpyCH4V TGCA 2 cut(s) 375, 449
HpyF10VI GCNNNNNNNGC 3 cut(s) 443, 583, 674
HpySE526I ACGT 2 cut(s) 485, 540
Hsp92II CATG 3 cut(s) 118, 403, 630
HspAI GCGC 1 cut(s) 97
Kzo9I GATC 2 cut(s) 253, 637
LmnI GCTCC 1 cut(s) 442
Lsp1109I GCAGC 2 cut(s) 391, 433
MaeII ACGT 2 cut(s) 485, 540
MaeIII GTNAC 3 cut(s) 62, 536, 541
MalI GATC 2 cut(s) 255, 639
MboI GATC 2 cut(s) 253, 637
MluCI AATT 3 cut(s) 86, 178, 687
MlyI GAGTC 2 cut(s) 53, 140
MmeI TCCRAC 1 cut(s) 27
MseI TTAA 3 cut(s) 12, 126, 736
MspA1I CMGCKG 1 cut(s) 155
MspI CCGG 3 cut(s) 60, 476, 521
MspR9I CCNGG 2 cut(s) 522, 651
MvaI CCWGG 1 cut(s) 651
MvnI CGCG 1 cut(s) 586
MwoI GCNNNNNNNGC 3 cut(s) 443, 583, 674
NciI CCSGG 1 cut(s) 522
NcoI CCATGG 1 cut(s) 399
NdeII GATC 2 cut(s) 253, 637
NlaIII CATG 3 cut(s) 118, 403, 630
NmeAIII GCCGAG 1 cut(s) 433
NmuCI GTSAC 2 cut(s) 536, 541
NspI RCATGY 1 cut(s) 118
PaeI GCATGC 1 cut(s) 118
PaeR7I CTCGAG 1 cut(s) 41
PcsI WCGNNNNNNNCGW 2 cut(s) 53, 558
PfeI GAWTC 3 cut(s) 288, 350, 380
PfoI TCCNGGA 1 cut(s) 649
PinAI ACCGGT 1 cut(s) 59
PkrI GCNGC 3 cut(s) 19, 406, 448
PleI GAGTC 2 cut(s) 52, 140
PpsI GAGTC 2 cut(s) 52, 140
Psp6I CCWGG 1 cut(s) 649
PspGI CCWGG 1 cut(s) 649
PspPI GGNCC 2 cut(s) 647, 676
PstI CTGCAG 1 cut(s) 451
PstNI CAGNNNCTG 1 cut(s) 291
RsaI GTAC 2 cut(s) 271, 594
RsaNI GTAC 2 cut(s) 270, 593
SaqAI TTAA 3 cut(s) 12, 126, 736
SatI GCNGC 3 cut(s) 18, 405, 447
Sau3AI GATC 2 cut(s) 253, 637
Sau96I GGNCC 2 cut(s) 647, 676
ScaI AGTACT 1 cut(s) 271
SchI GAGTC 2 cut(s) 53, 140
ScrFI CCNGG 2 cut(s) 522, 651
SetI ASST 8 cut(s) 233, 346, 423, 488, 538, 543, 670, 736
SfcI CTRYAG 1 cut(s) 447
Sfr274I CTCGAG 1 cut(s) 41
SinI GGWCC 1 cut(s) 647
SlaI CTCGAG 1 cut(s) 41
SmlI CTYRAG 2 cut(s) 41, 197
SmoI CTYRAG 2 cut(s) 41, 197
SphI GCATGC 1 cut(s) 118
Sse9I AATT 3 cut(s) 86, 178, 687
SsiI CCGC 4 cut(s) 18, 153, 429, 509
StyD4I CCNGG 2 cut(s) 520, 649
StyI CCWWGG 1 cut(s) 399
TaiI ACGT 2 cut(s) 488, 543
TaqI TCGA 4 cut(s) 42, 56, 101, 488
TasI AATT 3 cut(s) 86, 178, 687
TatI WGTACW 1 cut(s) 269
TauI GCSGC 1 cut(s) 20
TfiI GAWTC 3 cut(s) 288, 350, 380
Tru1I TTAA 3 cut(s) 12, 126, 736
Tru9I TTAA 3 cut(s) 12, 126, 736
TscAI CASTG 3 cut(s) 97, 179, 285
TseFI GTSAC 2 cut(s) 536, 541
TseI GCWGC 2 cut(s) 404, 446
Tsp45I GTSAC 2 cut(s) 536, 541
TspDTI ATGAA 2 cut(s) 341, 351
TspRI CASTG 3 cut(s) 97, 179, 285
VpaK11BI GGWCC 1 cut(s) 647
XapI RAATTY 3 cut(s) 86, 178, 687
XceI RCATGY 1 cut(s) 118
XhoI CTCGAG 1 cut(s) 41
ZrmI AGTACT 1 cut(s) 271
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.