Rw4G036310

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0009989
Physical Location & Seq
Reverse (-)
63162661 .. 63164875
2215 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G036310.1

Sequence Viewer

Length: 750 bp
ATGTCTTTAGTGATGGATGACTTTAATGCCGCACCACCATTTTCTTCTACTCTCGAGTCGGATTTCTCGACCGGTTACTTGGAAGACGCTTTGGTAGAATTCAGTGAGCGCTCGAAAAGAAGACGCATGCTGTTGTTTAACGATGACGAAATCAGAGACTCCACCGCTGTTGCTTCAAAGAGACACTGGAATTCAGACTGGGAAGTGCTTCAGAGTTTTGACAGCATTAGCCAACTAATCACCTCTACTGGGCTATCAGGGGATGATCCTACAAGTATGAGTACTACACCAGTGAGCAGAATCTGTGGGGATGAGACCAACATAGCCGATACAAAGATGAGCACAAGAGAAGAAGCTATTGATTCCTCGTCTTCATCTTCTTCTTGCAAAGAATCAGCCAACACCAACTCCATGGCTGCCGAGAAAGAGAACCGCTTTTCCGCTAATGCTCCTCTGGCTGCAGATGAGAAGAGAAATAAGAAGAGGCCGGTGATAACGTCGAGGGTGGTGTATCCCTTTGCGGTTGTGAAACCGGGAGGAGTAGAAGGTGACGTGACGCTAAACGACATCAACGAGAGGATTTCAATGCCACCAACACGACCAGTACGACACCCCGTTGGGGACTTTGCGTGTCGGCCATGCGTATCTGATCCTGATGGTCCAGGACTTTCTGGTAAAGCTGTGGTGGCCCTTACCAGAATTCAAACACAGGGAAGAGGCACAATCACTATTATCAGAACCAAAGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

249

Amino Acids

27.09

Weight (kDa)

5.24

Isoelectric Point (pI)

59.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009989)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14630 AT1G14630 AT2G01990 AT2G01990
fragaria_vesca FvH4_4g35920
malus_domestica MD13G1013900.v1.1 MD16G1011100.v1.1
prunus_persica Prupe.1G339600_v2.0.a1 Prupe.1G339600_v2.0.a1
pyrus_communis pycom13g01180 pycom16g01090
rosa_chinensis RchiOBHm_Chr4g0445521
rosa_laevigata RLG00000005733
rosa_multiflora Rmu_ssc0000253.1_g000002
rosa_roxburghii Rroxscaffold_5G00386000
rosa_rugosa Rorug04G0362100
rosa_samantha Rh4AG423600 Rh4BG435600 Rh4CG450300 Rh4DG431900
rosa_wichuraiana Rw4G036310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 30, 165, 433, 441, 521
AclWI GGATC 2 cut(s) 260, 644
AcoI YGGCCR 1 cut(s) 635
AcsI RAATTY 3 cut(s) 98, 190, 699
AcuI CTGAAG 1 cut(s) 194
AfaI GTAC 2 cut(s) 283, 606
AfeI AGCGCT 1 cut(s) 110
AfiI CCNNNNNNNGG 2 cut(s) 249, 619
AgeI ACCGGT 1 cut(s) 71
AgsI TTSAA 3 cut(s) 177, 585, 704
AjiI CACGTC 1 cut(s) 553
AjnI CCWGG 1 cut(s) 661
AloI GAACNNNNNNTCC 2 cut(s) 422, 454
AluBI AGCT 2 cut(s) 356, 680
AluI AGCT 2 cut(s) 356, 680
Alw21I GWGCWC 1 cut(s) 344
Alw26I GTCTC 3 cut(s) 150, 175, 308
AlwI GGATC 2 cut(s) 260, 644
AlwNI CAGNNNCTG 1 cut(s) 303
Ama87I CYCGRG 1 cut(s) 53
Aor51HI AGCGCT 1 cut(s) 110
AoxI GGCC 3 cut(s) 485, 635, 687
ApeKI GCWGC 2 cut(s) 416, 458
ApoI RAATTY 3 cut(s) 98, 190, 699
AsiGI ACCGGT 1 cut(s) 71
Asp700I GAANNNNTTC 1 cut(s) 207
AspLEI GCGC 1 cut(s) 111
AspS9I GGNCC 2 cut(s) 659, 688
AsuC2I CCSGG 1 cut(s) 534
AsuHPI GGTGA 3 cut(s) 232, 502, 560
AvaI CYCGRG 1 cut(s) 53
AvaII GGWCC 1 cut(s) 659
BbsI GAAGAC 3 cut(s) 90, 127, 363
Bbv12I GWGCWC 1 cut(s) 344
BbvI GCAGC 2 cut(s) 403, 445
BccI CCATC 2 cut(s) 7, 650
BciT130I CCWGG 1 cut(s) 663
BciVI GTATCC 1 cut(s) 522
BcnI CCSGG 1 cut(s) 534
BcoDI GTCTC 3 cut(s) 150, 175, 308
BfmI CTRYAG 1 cut(s) 459
BfoI RGCGCY 1 cut(s) 112
BfuI GTATCC 1 cut(s) 522
BisI GCNGC 3 cut(s) 30, 417, 459
BlsI GCNGC 3 cut(s) 31, 418, 460
BmcAI AGTACT 1 cut(s) 283
Bme1390I CCNGG 2 cut(s) 534, 663
Bme18I GGWCC 1 cut(s) 659
BmeT110I CYCGRG 1 cut(s) 53
BmgBI CACGTC 1 cut(s) 553
BmgT120I GGNCC 2 cut(s) 659, 688
BmrFI CCNGG 2 cut(s) 534, 663
BmrI ACTGGG 2 cut(s) 208, 258
BmuI ACTGGG 2 cut(s) 208, 258
BpiI GAAGAC 3 cut(s) 90, 127, 363
BpuMI CCSGG 1 cut(s) 534
BsaI GGTCTC 1 cut(s) 308
BsaJI CCNNGG 1 cut(s) 411
BsaWI WCCGGW 1 cut(s) 71
BsaXI ACNNNNNCTCC 2 cut(s) 392, 422
Bsc4I CCNNNNNNNGG 2 cut(s) 249, 619
Bse118I RCCGGY 2 cut(s) 71, 487
Bse1I ACTGG 5 cut(s) 191, 203, 253, 290, 602
BseBI CCWGG 1 cut(s) 663
BseDI CCNNGG 1 cut(s) 411
BseGI GGATG 3 cut(s) 22, 268, 316
BseLI CCNNNNNNNGG 2 cut(s) 249, 619
BseNI ACTGG 5 cut(s) 191, 203, 253, 290, 602
BseRI GAGGAG 2 cut(s) 441, 552
BseXI GCAGC 2 cut(s) 403, 445
Bsh1285I CGRYCG 1 cut(s) 72
BshFI GGCC 3 cut(s) 487, 637, 689
BshTI ACCGGT 1 cut(s) 71
BsiEI CGRYCG 1 cut(s) 72
BsiHKAI GWGCWC 1 cut(s) 344
BsiHKCI CYCGRG 1 cut(s) 53
BsiSI CCGG 3 cut(s) 72, 488, 533
BslFI GGGAC 1 cut(s) 635
BslI CCNNNNNNNGG 2 cut(s) 249, 619
BsmAI GTCTC 3 cut(s) 150, 175, 308
BsmFI GGGAC 1 cut(s) 635
BsnI GGCC 3 cut(s) 487, 637, 689
Bso31I GGTCTC 1 cut(s) 308
BsoBI CYCGRG 1 cut(s) 53
Bsp1286I GDGCHC 1 cut(s) 344
Bsp143I GATC 2 cut(s) 265, 649
Bsp19I CCATGG 1 cut(s) 411
BspACI CCGC 5 cut(s) 30, 165, 433, 441, 521
BspANI GGCC 3 cut(s) 487, 637, 689
BspMAI CTGCAG 1 cut(s) 463
BspPI GGATC 2 cut(s) 260, 644
BspTNI GGTCTC 1 cut(s) 308
BsrFI RCCGGY 2 cut(s) 71, 487
BsrI ACTGG 5 cut(s) 191, 203, 253, 290, 602
BssAI RCCGGY 2 cut(s) 71, 487
BssECI CCNNGG 1 cut(s) 411
BssMI GATC 2 cut(s) 265, 649
BssT1I CCWWGG 1 cut(s) 411
Bst2UI CCWGG 1 cut(s) 663
Bst6I CTCTTC 3 cut(s) 464, 476, 709
BstC8I GCNNGC 1 cut(s) 128
BstDSI CCRYGG 1 cut(s) 411
BstF5I GGATG 3 cut(s) 22, 268, 316
BstH2I RGCGCY 1 cut(s) 112
BstHHI GCGC 1 cut(s) 111
BstKTI GATC 2 cut(s) 268, 652
BstMAI GTCTC 3 cut(s) 150, 175, 308
BstMBI GATC 2 cut(s) 265, 649
BstMCI CGRYCG 1 cut(s) 72
BstMWI GCNNNNNNNGC 2 cut(s) 455, 686
BstNI CCWGG 1 cut(s) 663
BstNSI RCATGY 1 cut(s) 130
BstSCI CCNGG 2 cut(s) 532, 661
BstSFI CTRYAG 1 cut(s) 459
BstV1I GCAGC 2 cut(s) 403, 445
BstV2I GAAGAC 3 cut(s) 90, 127, 363
BstXI CCANNNNNNTGG 1 cut(s) 412
BsuI GTATCC 1 cut(s) 522
BsuRI GGCC 3 cut(s) 487, 637, 689
BtgI CCRYGG 1 cut(s) 411
BtrI CACGTC 1 cut(s) 553
BtsCI GGATG 3 cut(s) 22, 268, 316
BtsIMutI CAGTG 3 cut(s) 109, 184, 297
Cac8I GCNNGC 1 cut(s) 128
CaiI CAGNNNCTG 1 cut(s) 303
CfoI GCGC 1 cut(s) 111
Cfr10I RCCGGY 2 cut(s) 71, 487
Cfr13I GGNCC 2 cut(s) 659, 688
CseI GACGC 3 cut(s) 95, 132, 565
Csp6I GTAC 2 cut(s) 282, 605
CspAI ACCGGT 1 cut(s) 71
CspCI CAANNNNNGTGG 2 cut(s) 151, 186
CviAII CATG 3 cut(s) 127, 412, 639
CviQI GTAC 2 cut(s) 282, 605
DpnI GATC 2 cut(s) 267, 651
DpnII GATC 2 cut(s) 265, 649
EaeI YGGCCR 1 cut(s) 635
Eam1104I CTCTTC 3 cut(s) 464, 476, 709
EarI CTCTTC 3 cut(s) 464, 476, 709
Eco130I CCWWGG 1 cut(s) 411
Eco31I GGTCTC 1 cut(s) 308
Eco47I GGWCC 1 cut(s) 659
Eco47III AGCGCT 1 cut(s) 110
Eco57I CTGAAG 1 cut(s) 194
Eco88I CYCGRG 1 cut(s) 53
EcoRI GAATTC 3 cut(s) 98, 190, 699
EcoRII CCWGG 1 cut(s) 661
EcoT14I CCWWGG 1 cut(s) 411
ErhI CCWWGG 1 cut(s) 411
FaeI CATG 3 cut(s) 130, 415, 642
FaiI YATR 5 cut(s) 128, 278, 323, 413, 640
FaqI GGGAC 1 cut(s) 635
FatI CATG 3 cut(s) 126, 411, 638
Fnu4HI GCNGC 3 cut(s) 30, 417, 459
FokI GGATG 3 cut(s) 29, 275, 323
Fsp4HI GCNGC 3 cut(s) 30, 417, 459
GlaI GCGC 1 cut(s) 110
GluI GCNGC 3 cut(s) 30, 417, 459
HaeII RGCGCY 1 cut(s) 112
HaeIII GGCC 3 cut(s) 487, 637, 689
HapII CCGG 3 cut(s) 72, 488, 533
HgaI GACGC 3 cut(s) 95, 132, 565
HhaI GCGC 1 cut(s) 111
Hin1II CATG 3 cut(s) 130, 415, 642
Hin6I GCGC 1 cut(s) 109
HinP1I GCGC 1 cut(s) 109
HinfI GANTC 5 cut(s) 56, 158, 300, 362, 392
HpaII CCGG 3 cut(s) 72, 488, 533
HphI GGTGA 3 cut(s) 232, 502, 560
Hpy188I TCNGA 6 cut(s) 61, 155, 196, 213, 649, 737
Hpy188III TCNNGA 3 cut(s) 53, 67, 653
Hpy99I CGWCG 1 cut(s) 502
HpyAV CCTTC 1 cut(s) 539
HpyCH4IV ACGT 2 cut(s) 497, 552
HpyCH4V TGCA 2 cut(s) 387, 461
HpyF10VI GCNNNNNNNGC 2 cut(s) 455, 686
HpySE526I ACGT 2 cut(s) 497, 552
Hsp92II CATG 3 cut(s) 130, 415, 642
HspAI GCGC 1 cut(s) 109
Kzo9I GATC 2 cut(s) 265, 649
LmnI GCTCC 1 cut(s) 454
Lsp1109I GCAGC 2 cut(s) 403, 445
MaeII ACGT 2 cut(s) 497, 552
MaeIII GTNAC 3 cut(s) 74, 548, 553
MalI GATC 2 cut(s) 267, 651
MboI GATC 2 cut(s) 265, 649
MhlI GDGCHC 1 cut(s) 344
MluCI AATT 3 cut(s) 98, 190, 699
MlyI GAGTC 2 cut(s) 65, 152
MmeI TCCRAC 1 cut(s) 39
MnlI CCTC 8 cut(s) 253, 376, 462, 477, 495, 530, 570, 710
MroXI GAANNNNTTC 1 cut(s) 207
MseI TTAA 3 cut(s) 24, 138, 748
MspA1I CMGCKG 1 cut(s) 167
MspI CCGG 3 cut(s) 72, 488, 533
MspR9I CCNGG 2 cut(s) 534, 663
MvaI CCWGG 1 cut(s) 663
MwoI GCNNNNNNNGC 2 cut(s) 455, 686
NciI CCSGG 1 cut(s) 534
NcoI CCATGG 1 cut(s) 411
NdeII GATC 2 cut(s) 265, 649
NlaIII CATG 3 cut(s) 130, 415, 642
NmeAIII GCCGAG 1 cut(s) 445
NmuCI GTSAC 2 cut(s) 548, 553
NspI RCATGY 1 cut(s) 130
PaeI GCATGC 1 cut(s) 130
PaeR7I CTCGAG 1 cut(s) 53
PcsI WCGNNNNNNNCGW 3 cut(s) 65, 570, 604
PdmI GAANNNNTTC 1 cut(s) 207
PfeI GAWTC 3 cut(s) 300, 362, 392
PfoI TCCNGGA 1 cut(s) 661
PinAI ACCGGT 1 cut(s) 71
PkrI GCNGC 3 cut(s) 31, 418, 460
PleI GAGTC 2 cut(s) 64, 152
PpsI GAGTC 2 cut(s) 64, 152
Psp6I CCWGG 1 cut(s) 661
PspGI CCWGG 1 cut(s) 661
PspPI GGNCC 2 cut(s) 659, 688
PstI CTGCAG 1 cut(s) 463
PstNI CAGNNNCTG 1 cut(s) 303
RsaI GTAC 2 cut(s) 283, 606
RsaNI GTAC 2 cut(s) 282, 605
SaqAI TTAA 3 cut(s) 24, 138, 748
SatI GCNGC 3 cut(s) 30, 417, 459
Sau3AI GATC 2 cut(s) 265, 649
Sau96I GGNCC 2 cut(s) 659, 688
ScaI AGTACT 1 cut(s) 283
SchI GAGTC 2 cut(s) 65, 152
ScrFI CCNGG 2 cut(s) 534, 663
SduI GDGCHC 1 cut(s) 344
SetI ASST 7 cut(s) 245, 358, 500, 550, 555, 682, 748
SfcI CTRYAG 1 cut(s) 459
Sfr274I CTCGAG 1 cut(s) 53
SinI GGWCC 1 cut(s) 659
SlaI CTCGAG 1 cut(s) 53
SmlI CTYRAG 1 cut(s) 53
SmoI CTYRAG 1 cut(s) 53
SphI GCATGC 1 cut(s) 130
Sse9I AATT 3 cut(s) 98, 190, 699
SsiI CCGC 5 cut(s) 30, 165, 433, 441, 521
StyD4I CCNGG 2 cut(s) 532, 661
StyI CCWWGG 1 cut(s) 411
TaiI ACGT 2 cut(s) 500, 555
TaqI TCGA 4 cut(s) 54, 68, 113, 500
TasI AATT 3 cut(s) 98, 190, 699
TatI WGTACW 1 cut(s) 281
TauI GCSGC 1 cut(s) 32
TfiI GAWTC 3 cut(s) 300, 362, 392
Tru1I TTAA 3 cut(s) 24, 138, 748
Tru9I TTAA 3 cut(s) 24, 138, 748
TscAI CASTG 3 cut(s) 109, 191, 297
TseFI GTSAC 2 cut(s) 548, 553
TseI GCWGC 2 cut(s) 416, 458
Tsp45I GTSAC 2 cut(s) 548, 553
TspDTI ATGAA 1 cut(s) 363
TspRI CASTG 3 cut(s) 109, 191, 297
VpaK11BI GGWCC 1 cut(s) 659
XapI RAATTY 3 cut(s) 98, 190, 699
XceI RCATGY 1 cut(s) 130
XhoI CTCGAG 1 cut(s) 53
XmnI GAANNNNTTC 1 cut(s) 207
ZrmI AGTACT 1 cut(s) 283
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.