Rroxscaffold_5G00386000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0009989
Physical Location & Seq
Reverse (-)
64990999 .. 64992481
1483 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00386000.1

Sequence Viewer

Length: 873 bp
ATGGCTTACTCTAGCAGTAGCCTAGCCCTAGGAGGACCAGGACTAGGTCTAGGTCTAGGACTGGGAGGAGGGAATTGGGATAATTGCCACAACTTCAACCTTGGAGTTCTCAGCTCAGACAGGATGTCTTTAGTGATGGATGACTTTAATGCCGCACCACCATTTTCTTCTACTCTCGAGTCGGATTTCTCGACCGGTTACTTGGAAGACGCTTTGGTAGAATTCAGTGAGCGCTCGAAAAGAAGACGCATGCTGTTGTTTAACGATGACGAAATCAGAGACTCCACCGCTGTTGCTTCAAAGAAACACTGGAATTCAGACTGGGAAGTGCTTGAGAGTTTTGACAGCATCAGCCAACTAATCACCTCCACTGGGCTATCAGGGGATGATCCTACAAGTATGAGTACTACACCAGTGAGCAGACTCTGTGGGGATGAGACCAACATAGCCGATACAAAGATGAACACAAGAGAAGAAGCTATTGATTCCTCGTCTTCATCTTCTTCTTGCAAAGAATCAGGCAACACCAACTCCATGGCTGCCGAGAAAGAGAACCTCTTTTCCGCTAATGCTCCTCTGGCTGCAGATGAGAAGAGAAATAAGAAGAGGCCTGTGATAGCGTCGAGGGTGGTGTATCCATTTGCGGTGGTGAAACCGGGAGGAGTAGAAGGTGACGTGACGCTAAACGACATCAACGAGAGGATTTCAATGCCACCAACGCGACCAGTACGACACCCCGTTGGGGACTTTGCGTGTCGGCCATGCGTATCTGATCCTGATGGTCCAGGACTTTCTGGTAAAGCTGTGGTGGCCCTTACCAGAATTCAAACACAGGGAAGAGGCACAATCACTATTATCAGAACCAAAGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

290

Amino Acids

31.05

Weight (kDa)

5.04

Isoelectric Point (pI)

51.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009989)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14630 AT1G14630 AT2G01990 AT2G01990
fragaria_vesca FvH4_4g35920
malus_domestica MD13G1013900.v1.1 MD16G1011100.v1.1
prunus_persica Prupe.1G339600_v2.0.a1 Prupe.1G339600_v2.0.a1
pyrus_communis pycom13g01180 pycom16g01090
rosa_chinensis RchiOBHm_Chr4g0445521
rosa_laevigata RLG00000005733
rosa_multiflora Rmu_ssc0000253.1_g000002
rosa_roxburghii Rroxscaffold_5G00386000
rosa_rugosa Rorug04G0362100
rosa_samantha Rh4AG423600 Rh4BG435600 Rh4CG450300 Rh4DG431900
rosa_wichuraiana Rw4G036310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 721
AciI CCGC 4 cut(s) 153, 288, 564, 644
AclWI GGATC 2 cut(s) 383, 767
AcoI YGGCCR 1 cut(s) 758
AcsI RAATTY 3 cut(s) 221, 313, 822
AfaI GTAC 2 cut(s) 406, 729
AfeI AGCGCT 1 cut(s) 233
AfiI CCNNNNNNNGG 3 cut(s) 44, 372, 742
AgeI ACCGGT 1 cut(s) 194
AgsI TTSAA 4 cut(s) 97, 300, 708, 827
AhdI GACNNNNNGTC 1 cut(s) 124
AjiI CACGTC 1 cut(s) 676
AjnI CCWGG 2 cut(s) 37, 784
AloI GAACNNNNNNTCC 2 cut(s) 545, 577
AluBI AGCT 3 cut(s) 114, 479, 803
AluI AGCT 3 cut(s) 114, 479, 803
Alw26I GTCTC 2 cut(s) 273, 431
AlwI GGATC 2 cut(s) 383, 767
AlwNI CAGNNNCTG 1 cut(s) 426
Ama87I CYCGRG 1 cut(s) 176
Aor51HI AGCGCT 1 cut(s) 233
AoxI GGCC 3 cut(s) 608, 758, 810
ApeKI GCWGC 2 cut(s) 539, 581
ApoI RAATTY 3 cut(s) 221, 313, 822
AsiGI ACCGGT 1 cut(s) 194
AspA2I CCTAGG 1 cut(s) 28
AspLEI GCGC 1 cut(s) 234
AspS9I GGNCC 3 cut(s) 35, 782, 811
AsuC2I CCSGG 1 cut(s) 657
AsuHPI GGTGA 3 cut(s) 355, 661, 683
AvaI CYCGRG 1 cut(s) 176
AvaII GGWCC 2 cut(s) 35, 782
AvrII CCTAGG 1 cut(s) 28
BbsI GAAGAC 3 cut(s) 213, 250, 486
BbvI GCAGC 2 cut(s) 526, 568
BccI CCATC 2 cut(s) 130, 773
BciT130I CCWGG 2 cut(s) 39, 786
BciVI GTATCC 1 cut(s) 645
BcnI CCSGG 1 cut(s) 657
BcoDI GTCTC 2 cut(s) 273, 431
BfaI CTAG 6 cut(s) 12, 23, 29, 44, 50, 56
BfmI CTRYAG 1 cut(s) 582
BfoI RGCGCY 1 cut(s) 235
BfuI GTATCC 1 cut(s) 645
BisI GCNGC 3 cut(s) 153, 540, 582
BlnI CCTAGG 1 cut(s) 28
BlsI GCNGC 3 cut(s) 154, 541, 583
BmcAI AGTACT 1 cut(s) 406
Bme1390I CCNGG 3 cut(s) 39, 657, 786
Bme18I GGWCC 2 cut(s) 35, 782
BmeRI GACNNNNNGTC 1 cut(s) 124
BmeT110I CYCGRG 1 cut(s) 176
BmgBI CACGTC 1 cut(s) 676
BmgT120I GGNCC 3 cut(s) 35, 782, 811
BmrFI CCNGG 3 cut(s) 39, 657, 786
BmrI ACTGGG 3 cut(s) 71, 331, 381
BmsI GCATC 1 cut(s) 357
BmuI ACTGGG 3 cut(s) 71, 331, 381
BpiI GAAGAC 3 cut(s) 213, 250, 486
BpuEI CTTGAG 1 cut(s) 353
BpuMI CCSGG 1 cut(s) 657
BsaI GGTCTC 1 cut(s) 431
BsaJI CCNNGG 3 cut(s) 28, 100, 534
BsaWI WCCGGW 1 cut(s) 194
BsaXI ACNNNNNCTCC 2 cut(s) 515, 545
Bsc4I CCNNNNNNNGG 3 cut(s) 44, 372, 742
Bse118I RCCGGY 1 cut(s) 194
Bse1I ACTGG 6 cut(s) 66, 314, 326, 376, 413, 725
BseBI CCWGG 2 cut(s) 39, 786
BseDI CCNNGG 3 cut(s) 28, 100, 534
BseGI GGATG 4 cut(s) 129, 145, 391, 439
BseLI CCNNNNNNNGG 3 cut(s) 44, 372, 742
BseMII CTCAG 2 cut(s) 124, 129
BseNI ACTGG 6 cut(s) 66, 314, 326, 376, 413, 725
BseRI GAGGAG 3 cut(s) 81, 564, 675
BseXI GCAGC 2 cut(s) 526, 568
Bsh1236I CGCG 1 cut(s) 721
Bsh1285I CGRYCG 1 cut(s) 195
BshFI GGCC 3 cut(s) 610, 760, 812
BshTI ACCGGT 1 cut(s) 194
BsiEI CGRYCG 1 cut(s) 195
BsiHKCI CYCGRG 1 cut(s) 176
BsiSI CCGG 2 cut(s) 195, 656
BslFI GGGAC 1 cut(s) 758
BslI CCNNNNNNNGG 3 cut(s) 44, 372, 742
BsmAI GTCTC 2 cut(s) 273, 431
BsmFI GGGAC 1 cut(s) 758
BsnI GGCC 3 cut(s) 610, 760, 812
Bso31I GGTCTC 1 cut(s) 431
BsoBI CYCGRG 1 cut(s) 176
Bsp143I GATC 2 cut(s) 388, 772
Bsp19I CCATGG 1 cut(s) 534
BspACI CCGC 4 cut(s) 153, 288, 564, 644
BspANI GGCC 3 cut(s) 610, 760, 812
BspCNI CTCAG 2 cut(s) 123, 128
BspFNI CGCG 1 cut(s) 721
BspMAI CTGCAG 1 cut(s) 586
BspPI GGATC 2 cut(s) 383, 767
BspTNI GGTCTC 1 cut(s) 431
BsrFI RCCGGY 1 cut(s) 194
BsrI ACTGG 6 cut(s) 66, 314, 326, 376, 413, 725
BssAI RCCGGY 1 cut(s) 194
BssECI CCNNGG 3 cut(s) 28, 100, 534
BssMI GATC 2 cut(s) 388, 772
BssT1I CCWWGG 3 cut(s) 28, 100, 534
Bst2UI CCWGG 2 cut(s) 39, 786
Bst6I CTCTTC 3 cut(s) 587, 599, 832
BstC8I GCNNGC 1 cut(s) 251
BstDEI CTNAG 2 cut(s) 110, 115
BstDSI CCRYGG 1 cut(s) 534
BstF5I GGATG 4 cut(s) 129, 145, 391, 439
BstFNI CGCG 1 cut(s) 721
BstH2I RGCGCY 1 cut(s) 235
BstHHI GCGC 1 cut(s) 234
BstKTI GATC 2 cut(s) 391, 775
BstMAI GTCTC 2 cut(s) 273, 431
BstMBI GATC 2 cut(s) 388, 772
BstMCI CGRYCG 1 cut(s) 195
BstMWI GCNNNNNNNGC 3 cut(s) 578, 718, 809
BstNI CCWGG 2 cut(s) 39, 786
BstNSI RCATGY 1 cut(s) 253
BstSCI CCNGG 3 cut(s) 37, 655, 784
BstSFI CTRYAG 1 cut(s) 582
BstUI CGCG 1 cut(s) 721
BstV1I GCAGC 2 cut(s) 526, 568
BstV2I GAAGAC 3 cut(s) 213, 250, 486
BstXI CCANNNNNNTGG 1 cut(s) 535
BsuI GTATCC 1 cut(s) 645
BsuRI GGCC 3 cut(s) 610, 760, 812
BtgI CCRYGG 1 cut(s) 534
BtrI CACGTC 1 cut(s) 676
BtsCI GGATG 4 cut(s) 129, 145, 391, 439
BtsIMutI CAGTG 4 cut(s) 232, 307, 369, 420
Cac8I GCNNGC 1 cut(s) 251
CaiI CAGNNNCTG 1 cut(s) 426
CfoI GCGC 1 cut(s) 234
Cfr10I RCCGGY 1 cut(s) 194
Cfr13I GGNCC 3 cut(s) 35, 782, 811
CseI GACGC 4 cut(s) 218, 255, 609, 688
Csp6I GTAC 2 cut(s) 405, 728
CspAI ACCGGT 1 cut(s) 194
CspCI CAANNNNNGTGG 2 cut(s) 274, 309
CviAII CATG 3 cut(s) 250, 535, 762
CviQI GTAC 2 cut(s) 405, 728
DdeI CTNAG 2 cut(s) 110, 115
DpnI GATC 2 cut(s) 390, 774
DpnII GATC 2 cut(s) 388, 772
DriI GACNNNNNGTC 1 cut(s) 124
EaeI YGGCCR 1 cut(s) 758
Eam1104I CTCTTC 3 cut(s) 587, 599, 832
Eam1105I GACNNNNNGTC 1 cut(s) 124
EarI CTCTTC 3 cut(s) 587, 599, 832
Eco130I CCWWGG 3 cut(s) 28, 100, 534
Eco147I AGGCCT 1 cut(s) 610
Eco31I GGTCTC 1 cut(s) 431
Eco47I GGWCC 2 cut(s) 35, 782
Eco47III AGCGCT 1 cut(s) 233
Eco88I CYCGRG 1 cut(s) 176
EcoRI GAATTC 3 cut(s) 221, 313, 822
EcoRII CCWGG 2 cut(s) 37, 784
EcoT14I CCWWGG 3 cut(s) 28, 100, 534
ErhI CCWWGG 3 cut(s) 28, 100, 534
FaeI CATG 3 cut(s) 253, 538, 765
FaiI YATR 5 cut(s) 251, 401, 446, 536, 763
FaqI GGGAC 1 cut(s) 758
FatI CATG 3 cut(s) 249, 534, 761
Fnu4HI GCNGC 3 cut(s) 153, 540, 582
FokI GGATG 4 cut(s) 136, 152, 398, 446
Fsp4HI GCNGC 3 cut(s) 153, 540, 582
FspBI CTAG 6 cut(s) 12, 23, 29, 44, 50, 56
GlaI GCGC 1 cut(s) 233
GluI GCNGC 3 cut(s) 153, 540, 582
HaeII RGCGCY 1 cut(s) 235
HaeIII GGCC 3 cut(s) 610, 760, 812
HapII CCGG 2 cut(s) 195, 656
HgaI GACGC 4 cut(s) 218, 255, 609, 688
HhaI GCGC 1 cut(s) 234
Hin1II CATG 3 cut(s) 253, 538, 765
Hin6I GCGC 1 cut(s) 232
HinP1I GCGC 1 cut(s) 232
HinfI GANTC 5 cut(s) 179, 281, 423, 485, 515
HpaII CCGG 2 cut(s) 195, 656
HphI GGTGA 3 cut(s) 355, 661, 683
Hpy188I TCNGA 6 cut(s) 118, 184, 278, 319, 772, 860
Hpy188III TCNNGA 3 cut(s) 176, 190, 776
Hpy99I CGWCG 1 cut(s) 625
HpyAV CCTTC 1 cut(s) 662
HpyCH4IV ACGT 1 cut(s) 675
HpyCH4V TGCA 2 cut(s) 510, 584
HpyF10VI GCNNNNNNNGC 3 cut(s) 578, 718, 809
HpyF3I CTNAG 2 cut(s) 110, 115
HpySE526I ACGT 1 cut(s) 675
Hsp92II CATG 3 cut(s) 253, 538, 765
HspAI GCGC 1 cut(s) 232
Kzo9I GATC 2 cut(s) 388, 772
LmnI GCTCC 1 cut(s) 577
Lsp1109I GCAGC 2 cut(s) 526, 568
LweI GCATC 1 cut(s) 357
MaeI CTAG 6 cut(s) 12, 23, 29, 44, 50, 56
MaeII ACGT 1 cut(s) 675
MaeIII GTNAC 3 cut(s) 197, 671, 676
MalI GATC 2 cut(s) 390, 774
MboI GATC 2 cut(s) 388, 772
MluCI AATT 5 cut(s) 73, 82, 221, 313, 822
MlyI GAGTC 3 cut(s) 188, 275, 417
MmeI TCCRAC 1 cut(s) 162
MseI TTAA 3 cut(s) 147, 261, 871
MspA1I CMGCKG 1 cut(s) 290
MspI CCGG 2 cut(s) 195, 656
MspR9I CCNGG 3 cut(s) 39, 657, 786
MvaI CCWGG 2 cut(s) 39, 786
MvnI CGCG 1 cut(s) 721
MwoI GCNNNNNNNGC 3 cut(s) 578, 718, 809
NciI CCSGG 1 cut(s) 657
NcoI CCATGG 1 cut(s) 534
NdeII GATC 2 cut(s) 388, 772
NlaIII CATG 3 cut(s) 253, 538, 765
NmeAIII GCCGAG 1 cut(s) 568
NmuCI GTSAC 2 cut(s) 671, 676
NspI RCATGY 1 cut(s) 253
PaeI GCATGC 1 cut(s) 253
PaeR7I CTCGAG 1 cut(s) 176
PceI AGGCCT 1 cut(s) 610
PcsI WCGNNNNNNNCGW 2 cut(s) 188, 693
PfeI GAWTC 2 cut(s) 485, 515
PflFI GACNNNGTC 1 cut(s) 45
PfoI TCCNGGA 1 cut(s) 784
PinAI ACCGGT 1 cut(s) 194
PkrI GCNGC 3 cut(s) 154, 541, 583
PleI GAGTC 3 cut(s) 187, 275, 417
PpsI GAGTC 3 cut(s) 187, 275, 417
Psp6I CCWGG 2 cut(s) 37, 784
PspGI CCWGG 2 cut(s) 37, 784
PspPI GGNCC 3 cut(s) 35, 782, 811
PstI CTGCAG 1 cut(s) 586
PstNI CAGNNNCTG 1 cut(s) 426
PsyI GACNNNGTC 1 cut(s) 45
RsaI GTAC 2 cut(s) 406, 729
RsaNI GTAC 2 cut(s) 405, 728
SaqAI TTAA 3 cut(s) 147, 261, 871
SatI GCNGC 3 cut(s) 153, 540, 582
Sau3AI GATC 2 cut(s) 388, 772
Sau96I GGNCC 3 cut(s) 35, 782, 811
ScaI AGTACT 1 cut(s) 406
SchI GAGTC 3 cut(s) 188, 275, 417
ScrFI CCNGG 3 cut(s) 39, 657, 786
SfaNI GCATC 1 cut(s) 357
SfcI CTRYAG 1 cut(s) 582
Sfr274I CTCGAG 1 cut(s) 176
SinI GGWCC 2 cut(s) 35, 782
SlaI CTCGAG 1 cut(s) 176
SmlI CTYRAG 2 cut(s) 176, 332
SmoI CTYRAG 2 cut(s) 176, 332
SphI GCATGC 1 cut(s) 253
Sse9I AATT 5 cut(s) 73, 82, 221, 313, 822
SseBI AGGCCT 1 cut(s) 610
SsiI CCGC 4 cut(s) 153, 288, 564, 644
SspMI CTAG 6 cut(s) 12, 23, 29, 44, 50, 56
StuI AGGCCT 1 cut(s) 610
StyD4I CCNGG 3 cut(s) 37, 655, 784
StyI CCWWGG 3 cut(s) 28, 100, 534
TaiI ACGT 1 cut(s) 678
TaqI TCGA 4 cut(s) 177, 191, 236, 623
TasI AATT 5 cut(s) 73, 82, 221, 313, 822
TatI WGTACW 1 cut(s) 404
TauI GCSGC 1 cut(s) 155
TfiI GAWTC 2 cut(s) 485, 515
Tru1I TTAA 3 cut(s) 147, 261, 871
Tru9I TTAA 3 cut(s) 147, 261, 871
TscAI CASTG 4 cut(s) 232, 314, 376, 420
TseFI GTSAC 2 cut(s) 671, 676
TseI GCWGC 2 cut(s) 539, 581
Tsp45I GTSAC 2 cut(s) 671, 676
TspDTI ATGAA 2 cut(s) 476, 486
TspRI CASTG 4 cut(s) 232, 314, 376, 420
Tth111I GACNNNGTC 1 cut(s) 45
VpaK11BI GGWCC 2 cut(s) 35, 782
XapI RAATTY 3 cut(s) 221, 313, 822
XceI RCATGY 1 cut(s) 253
XhoI CTCGAG 1 cut(s) 176
XmaJI CCTAGG 1 cut(s) 28
XspI CTAG 6 cut(s) 12, 23, 29, 44, 50, 56
ZrmI AGTACT 1 cut(s) 406
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.