FvH4_4g36920

ATP-dependent Clp protease proteolytic subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0014077
Physical Location & Seq
Forward (+)
33641955 .. 33644679
2725 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g36920.t1

Sequence Viewer

Length: 729 bp
ATGATGAGGAGCCTCATCTCAGGCGCCAAGCTCGTGGCGACCAGCAGAGCACGATGGGGAACGACGACGCGGTGCTACAGTCTGATCCCAATGGTGATAGAGCACTCGTCTCGAGGTGAGCGAGCATACGACATCTTCTCTCGCCTTCTCAAGGAGCGCATAATCTGCATCAACGGTCCCATCAACGACGACACCGCCCATGTCGTTGTCGCTCAGCTTCTCTTTCTCGAGTCCGAGAATCCTTCCAAGCCCATCCATATGTACCTCAATTCTCCCGGTGGCCATGTCACTGCAGGTCTTGCAATTTATGATACTATGCAGTACATACGGTCTCCAATCAATACAATTTGTTTGGGCCAAGCTGCATCAATGGCTTCACTTCTCTTAGCTGCGGGTGCCAAGGGTGAGAGGCGGTCACTACCCAATGCGACGATTATGATTCATCAGCCTTCAGGTGGGTACAGTGGACAAGCAAAAGATATGACGATTCACACAAAGCAGATTGTCCGTGTGTGGGATTCACTCAATGCATTGTATTCAAAGCATACAGGACAGTCAAAAGAGATAATTGAGAAGAATATGGATAGGGATTACTTCATGACCCCAGAAGAGGCCAAGAAGTTTGGAATAATTGATGAAGTGATTGATGAAAGACCAATGGCTCTGGTCACTGATGCTGTTGGCAAAGAAGGCAAAGAAAGCAAAGAAAAGGATAAGAGTTCAAACTAG

Protein Analysis

243

Amino Acids

26.79

Weight (kDa)

8.67

Isoelectric Point (pI)

38.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLP_protease PF00574 38 - 217 4.1e-82 Clp protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014077)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23140
fragaria_vesca FvH4_4g36920
malus_domestica MD13G1005300.v1.1 MD16G1002600.v1.1
prunus_persica Prupe.1G347500_v2.0.a1
pyrus_communis pycom13g00450 pycom16g00330
rosa_chinensis RchiOBHm_Chr4g0446681
rosa_laevigata RLG00000005640
rosa_multiflora Rmu_sc0001753.1_g000041
rosa_roxburghii Rroxscaffold_5G00387040
rosa_rugosa Rorug04G0373600
rosa_samantha Rh4AG435100 Rh4BG444100 Rh4CG461400 Rh4DG442900
rosa_wichuraiana Rw4G037200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 284
AccB1I GGYRCC 2 cut(s) 23, 395
AccII CGCG 1 cut(s) 70
AciI CCGC 4 cut(s) 70, 195, 392, 412
AclWI GGATC 1 cut(s) 79
AcoI YGGCCR 1 cut(s) 280
AcuI CTGAAG 1 cut(s) 435
AcyI GRCGYC 1 cut(s) 24
AfaI GTAC 3 cut(s) 263, 323, 461
AfiI CCNNNNNNNGG 4 cut(s) 151, 455, 514, 610
AgsI TTSAA 2 cut(s) 540, 723
AluBI AGCT 4 cut(s) 31, 217, 362, 389
AluI AGCT 4 cut(s) 31, 217, 362, 389
Alw21I GWGCWC 2 cut(s) 52, 105
Alw26I GTCTC 2 cut(s) 114, 336
AlwI GGATC 1 cut(s) 79
Ama87I CYCGRG 2 cut(s) 111, 227
AoxI GGCC 3 cut(s) 280, 355, 612
ApeKI GCWGC 2 cut(s) 362, 389
AspLEI GCGC 2 cut(s) 26, 159
AspS9I GGNCC 2 cut(s) 176, 355
AsuC2I CCSGG 1 cut(s) 276
AsuHPI GGTGA 3 cut(s) 106, 128, 416
AvaI CYCGRG 2 cut(s) 111, 227
AvaII GGWCC 1 cut(s) 176
BalI TGGCCA 1 cut(s) 282
BanI GGYRCC 2 cut(s) 23, 395
BauI CACGAG 1 cut(s) 32
Bbv12I GWGCWC 2 cut(s) 52, 105
BbvI GCAGC 2 cut(s) 349, 376
BccI CCATC 3 cut(s) 48, 188, 260
BcgI CGANNNNNNTGC 2 cut(s) 92, 126
BcnI CCSGG 1 cut(s) 276
BcoDI GTCTC 2 cut(s) 114, 336
BfaI CTAG 1 cut(s) 727
BfmI CTRYAG 2 cut(s) 76, 291
BfoI RGCGCY 1 cut(s) 27
BfuAI ACCTGC 1 cut(s) 284
BisI GCNGC 2 cut(s) 363, 390
BlpI GCTNAGC 1 cut(s) 213
BlsI GCNGC 2 cut(s) 364, 391
Bme1390I CCNGG 1 cut(s) 276
Bme18I GGWCC 1 cut(s) 176
BmeT110I CYCGRG 2 cut(s) 111, 227
BmgT120I GGNCC 2 cut(s) 176, 355
BmiI GGNNCC 4 cut(s) 11, 25, 178, 397
BmrFI CCNGG 1 cut(s) 276
BmsI GCATC 3 cut(s) 177, 374, 664
Bpu1102I GCTNAGC 1 cut(s) 213
BpuEI CTTGAG 1 cut(s) 134
BpuMI CCSGG 1 cut(s) 276
BsaHI GRCGYC 1 cut(s) 24
BsaI GGTCTC 1 cut(s) 336
BsaJI CCNNGG 1 cut(s) 399
Bsc4I CCNNNNNNNGG 4 cut(s) 151, 455, 514, 610
BseDI CCNNGG 1 cut(s) 399
BseGI GGATG 1 cut(s) 252
BseLI CCNNNNNNNGG 4 cut(s) 151, 455, 514, 610
BseMII CTCAG 2 cut(s) 33, 227
BseRI GAGGAG 1 cut(s) 22
BseXI GCAGC 2 cut(s) 349, 376
Bsh1236I CGCG 1 cut(s) 70
BshFI GGCC 3 cut(s) 282, 357, 614
BshNI GGYRCC 2 cut(s) 23, 395
BsiHKAI GWGCWC 2 cut(s) 52, 105
BsiHKCI CYCGRG 2 cut(s) 111, 227
BsiSI CCGG 1 cut(s) 276
BslFI GGGAC 1 cut(s) 162
BslI CCNNNNNNNGG 4 cut(s) 151, 455, 514, 610
BsmAI GTCTC 2 cut(s) 114, 336
BsmBI CGTCTC 1 cut(s) 114
BsmFI GGGAC 1 cut(s) 162
BsnI GGCC 3 cut(s) 282, 357, 614
Bso31I GGTCTC 1 cut(s) 336
BsoBI CYCGRG 2 cut(s) 111, 227
Bsp1286I GDGCHC 2 cut(s) 52, 105
Bsp143I GATC 1 cut(s) 84
Bsp1720I GCTNAGC 1 cut(s) 213
BspACI CCGC 4 cut(s) 70, 195, 392, 412
BspANI GGCC 3 cut(s) 282, 357, 614
BspCNI CTCAG 2 cut(s) 32, 226
BspFNI CGCG 1 cut(s) 70
BspHI TCATGA 1 cut(s) 597
BspLI GGNNCC 4 cut(s) 11, 25, 178, 397
BspMAI CTGCAG 1 cut(s) 295
BspMI ACCTGC 1 cut(s) 284
BspPI GGATC 1 cut(s) 79
BspT107I GGYRCC 2 cut(s) 23, 395
BspTNI GGTCTC 1 cut(s) 336
BssECI CCNNGG 1 cut(s) 399
BssMI GATC 1 cut(s) 84
BssNI GRCGYC 1 cut(s) 24
BssSI CACGAG 1 cut(s) 32
BssT1I CCWWGG 1 cut(s) 399
Bst2BI CACGAG 1 cut(s) 32
Bst4CI ACNGT 5 cut(s) 80, 176, 330, 464, 555
Bst6I CTCTTC 1 cut(s) 603
BstACI GRCGYC 1 cut(s) 24
BstAPI GCANNNNNTGC 2 cut(s) 165, 299
BstC8I GCNNGC 1 cut(s) 123
BstDEI CTNAG 3 cut(s) 19, 213, 385
BstENI CCTNNNNNAGG 1 cut(s) 149
BstF5I GGATG 1 cut(s) 252
BstFNI CGCG 1 cut(s) 70
BstH2I RGCGCY 1 cut(s) 27
BstHHI GCGC 2 cut(s) 26, 159
BstKTI GATC 1 cut(s) 87
BstMAI GTCTC 2 cut(s) 114, 336
BstMBI GATC 1 cut(s) 84
BstMWI GCNNNNNNNGC 6 cut(s) 165, 299, 371, 395, 690, 699
BstSCI CCNGG 1 cut(s) 274
BstSFI CTRYAG 2 cut(s) 76, 291
BstUI CGCG 1 cut(s) 70
BstV1I GCAGC 2 cut(s) 349, 376
BstXI CCANNNNNNTGG 1 cut(s) 34
BsuRI GGCC 3 cut(s) 282, 357, 614
BtsCI GGATG 1 cut(s) 252
BtsI GCAGTG 1 cut(s) 288
BtsIMutI CAGTG 3 cut(s) 288, 469, 669
BveI ACCTGC 1 cut(s) 284
Cac8I GCNNGC 1 cut(s) 123
CciI TCATGA 1 cut(s) 597
CfoI GCGC 2 cut(s) 26, 159
Cfr13I GGNCC 2 cut(s) 176, 355
CseI GACGC 1 cut(s) 76
Csp6I GTAC 3 cut(s) 262, 322, 460
CviAII CATG 3 cut(s) 200, 284, 598
CviQI GTAC 3 cut(s) 262, 322, 460
DdeI CTNAG 3 cut(s) 19, 213, 385
DinI GGCGCC 1 cut(s) 25
DpnI GATC 1 cut(s) 86
DpnII GATC 1 cut(s) 84
EaeI YGGCCR 1 cut(s) 280
Eam1104I CTCTTC 1 cut(s) 603
EarI CTCTTC 1 cut(s) 603
Eco130I CCWWGG 1 cut(s) 399
Eco31I GGTCTC 1 cut(s) 336
Eco47I GGWCC 1 cut(s) 176
Eco57I CTGAAG 1 cut(s) 435
Eco88I CYCGRG 2 cut(s) 111, 227
EcoNI CCTNNNNNAGG 1 cut(s) 149
EcoT14I CCWWGG 1 cut(s) 399
EcoT22I ATGCAT 1 cut(s) 532
EgeI GGCGCC 1 cut(s) 25
EheI GGCGCC 1 cut(s) 25
ErhI CCWWGG 1 cut(s) 399
Esp3I CGTCTC 1 cut(s) 114
FaeI CATG 3 cut(s) 203, 287, 601
FaqI GGGAC 1 cut(s) 162
FatI CATG 3 cut(s) 199, 283, 597
FauI CCCGC 1 cut(s) 385
FauNDI CATATG 1 cut(s) 258
Fnu4HI GCNGC 2 cut(s) 363, 390
FokI GGATG 1 cut(s) 239
Fsp4HI GCNGC 2 cut(s) 363, 390
FspBI CTAG 1 cut(s) 727
GlaI GCGC 2 cut(s) 25, 158
GluI GCNGC 2 cut(s) 363, 390
HaeII RGCGCY 1 cut(s) 27
HaeIII GGCC 3 cut(s) 282, 357, 614
HapII CCGG 1 cut(s) 276
HgaI GACGC 1 cut(s) 76
HhaI GCGC 2 cut(s) 26, 159
Hin1I GRCGYC 1 cut(s) 24
Hin1II CATG 3 cut(s) 203, 287, 601
Hin6I GCGC 2 cut(s) 24, 157
HinP1I GCGC 2 cut(s) 24, 157
HinfI GANTC 5 cut(s) 230, 238, 439, 487, 518
HpaII CCGG 1 cut(s) 276
HphI GGTGA 3 cut(s) 106, 128, 416
Hpy166II GTNNAC 1 cut(s) 467
Hpy188I TCNGA 2 cut(s) 84, 235
Hpy188III TCNNGA 3 cut(s) 111, 227, 598
Hpy8I GTNNAC 1 cut(s) 467
Hpy99I CGWCG 4 cut(s) 67, 70, 191, 433
HpyAV CCTTC 4 cut(s) 155, 252, 459, 683
HpyCH4III ACNGT 5 cut(s) 80, 176, 330, 464, 555
HpyCH4V TGCA 6 cut(s) 168, 293, 302, 319, 365, 530
HpyF10VI GCNNNNNNNGC 6 cut(s) 165, 299, 371, 395, 690, 699
HpyF3I CTNAG 3 cut(s) 19, 213, 385
Hsp92I GRCGYC 1 cut(s) 24
Hsp92II CATG 3 cut(s) 203, 287, 601
HspAI GCGC 2 cut(s) 24, 157
KasI GGCGCC 1 cut(s) 23
Kzo9I GATC 1 cut(s) 84
LmnI GCTCC 2 cut(s) 9, 154
LpnPI CCDG 8 cut(s) 6, 55, 279, 289, 438, 534, 618, 650
Lsp1109I GCAGC 2 cut(s) 349, 376
LweI GCATC 3 cut(s) 177, 374, 664
MaeI CTAG 1 cut(s) 727
MaeIII GTNAC 3 cut(s) 286, 414, 667
MalI GATC 1 cut(s) 86
MboI GATC 1 cut(s) 84
MboII GAAGA 3 cut(s) 127, 586, 620
MhlI GDGCHC 2 cut(s) 52, 105
MlsI TGGCCA 1 cut(s) 282
MluCI AATT 5 cut(s) 268, 303, 345, 567, 630
MluNI TGGCCA 1 cut(s) 282
Mly113I GGCGCC 1 cut(s) 24
MlyI GAGTC 1 cut(s) 239
MnlI CCTC 5 cut(s) 23, 107, 275, 402, 604
Mox20I TGGCCA 1 cut(s) 282
Mph1103I ATGCAT 1 cut(s) 532
MscI TGGCCA 1 cut(s) 282
MslI CAYNNNNRTG 1 cut(s) 257
Msp20I TGGCCA 1 cut(s) 282
MspI CCGG 1 cut(s) 276
MspR9I CCNGG 1 cut(s) 276
MvnI CGCG 1 cut(s) 70
MwoI GCNNNNNNNGC 6 cut(s) 165, 299, 371, 395, 690, 699
NarI GGCGCC 1 cut(s) 24
NciI CCSGG 1 cut(s) 276
NdeI CATATG 1 cut(s) 258
NdeII GATC 1 cut(s) 84
NlaIII CATG 3 cut(s) 203, 287, 601
NlaIV GGNNCC 4 cut(s) 11, 25, 178, 397
NmuCI GTSAC 3 cut(s) 286, 414, 667
NsiI ATGCAT 1 cut(s) 532
PaeR7I CTCGAG 2 cut(s) 111, 227
PagI TCATGA 1 cut(s) 597
PcsI WCGNNNNNNNCGW 1 cut(s) 118
PfeI GAWTC 4 cut(s) 238, 439, 487, 518
PkrI GCNGC 2 cut(s) 364, 391
PleI GAGTC 1 cut(s) 238
PluTI GGCGCC 1 cut(s) 27
PpsI GAGTC 1 cut(s) 238
PspN4I GGNNCC 4 cut(s) 11, 25, 178, 397
PspPI GGNCC 2 cut(s) 176, 355
PstI CTGCAG 1 cut(s) 295
RsaI GTAC 3 cut(s) 263, 323, 461
RsaNI GTAC 3 cut(s) 262, 322, 460
RseI CAYNNNNRTG 1 cut(s) 257
SatI GCNGC 2 cut(s) 363, 390
Sau3AI GATC 1 cut(s) 84
Sau96I GGNCC 2 cut(s) 176, 355
SchI GAGTC 1 cut(s) 239
ScrFI CCNGG 1 cut(s) 276
SduI GDGCHC 2 cut(s) 52, 105
SetI ASST 8 cut(s) 33, 118, 219, 267, 298, 364, 391, 457
SfaNI GCATC 3 cut(s) 177, 374, 664
SfcI CTRYAG 2 cut(s) 76, 291
SfoI GGCGCC 1 cut(s) 25
Sfr274I CTCGAG 2 cut(s) 111, 227
SinI GGWCC 1 cut(s) 176
SlaI CTCGAG 2 cut(s) 111, 227
SmiMI CAYNNNNRTG 1 cut(s) 257
SmlI CTYRAG 3 cut(s) 111, 149, 227
SmoI CTYRAG 3 cut(s) 111, 149, 227
Sse9I AATT 5 cut(s) 268, 303, 345, 567, 630
SsiI CCGC 4 cut(s) 70, 195, 392, 412
SspDI GGCGCC 1 cut(s) 23
SspMI CTAG 1 cut(s) 727
StyD4I CCNGG 1 cut(s) 274
StyI CCWWGG 1 cut(s) 399
TaaI ACNGT 5 cut(s) 80, 176, 330, 464, 555
TaqI TCGA 2 cut(s) 112, 228
TasI AATT 5 cut(s) 268, 303, 345, 567, 630
TatI WGTACW 1 cut(s) 321
TfiI GAWTC 4 cut(s) 238, 439, 487, 518
TscAI CASTG 3 cut(s) 295, 469, 676
TseFI GTSAC 3 cut(s) 286, 414, 667
TseI GCWGC 2 cut(s) 362, 389
Tsp45I GTSAC 3 cut(s) 286, 414, 667
TspDTI ATGAA 4 cut(s) 431, 586, 651, 663
TspGWI ACGGA 1 cut(s) 497
TspRI CASTG 3 cut(s) 295, 469, 676
VpaK11BI GGWCC 1 cut(s) 176
XagI CCTNNNNNAGG 1 cut(s) 149
XhoI CTCGAG 2 cut(s) 111, 227
XspI CTAG 1 cut(s) 727
Zsp2I ATGCAT 1 cut(s) 532
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.