pycom16g00330

ATP-dependent Clp protease proteolytic subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0014077
Physical Location & Seq
Forward (+)
176542 .. 178943
2402 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g00330.1

Sequence Viewer

Length: 714 bp
ATGAGAGGCTTAATCTCAAACGCGAAGCTCCTGGCAAGCAGCAGGCCATGGGACCCATCTCTGCGCAGCTACAGCTTGATTCCGATGGTCATTGAGCACTCCTCCCGAGGTGAACGCGCGTACGACATCTTCTCCCGCCTCCTCAAGGAGCGGATCATCTGCATCAACGGCCCCATCAACGACGACACCTCCCACGTCGTCGTCGCGCAGCTTCTCTTCTTGGAATCCGAGAACCCGTCCAAGCCCATCCACATGTACCTCAATTCCCCCGGTGGTCACGTCACTGCAGGTCTTGCAATTTATGATACGATGCAGTACATTCGGTCACCAATCAGTACCATTTGTTTGGGCCAAGCTGCATCAATGGCTTCTCTTCTCTTAGCTGCGGGCGCCAAGGGTGAGAGGCGGTCTCTCCCAAATGCTTCAGTTATGATTCATCAGCCTTCAGGCGGGTACAGCGGACAAGCAAAAGATATGACAATTCACACGAAGCAGATGGTTCGTGTCTGGGATTCCCTTAATGCGTTGTACTCGAAGCATACGGGGCAACCAGTAGATGTAGTTGAGAAGAATATGGACAGGGATAATTTTATGACCCCAGAAGAGGCAAAGGCATTCGGAATCATTGATGAGGTGATTGATGAAAGACCAATGGCTCTGGTGACTGATGCTGTTGCCAATGAAGGCAAAGAAAAGGATAAAAGTTCAAAGTAG

Protein Analysis

238

Amino Acids

26.07

Weight (kDa)

6.66

Isoelectric Point (pI)

37.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLP_protease PF00574 36 - 215 6.9e-81 Clp protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014077)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23140
fragaria_vesca FvH4_4g36920
malus_domestica MD13G1005300.v1.1 MD16G1002600.v1.1
prunus_persica Prupe.1G347500_v2.0.a1
pyrus_communis pycom13g00450 pycom16g00330
rosa_chinensis RchiOBHm_Chr4g0446681
rosa_laevigata RLG00000005640
rosa_multiflora Rmu_sc0001753.1_g000041
rosa_roxburghii Rroxscaffold_5G00387040
rosa_rugosa Rorug04G0373600
rosa_samantha Rh4AG435100 Rh4BG444100 Rh4CG461400 Rh4DG442900
rosa_wichuraiana Rw4G037200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 65
Acc36I ACCTGC 1 cut(s) 278
AccB1I GGYRCC 1 cut(s) 389
AccBSI CCGCTC 1 cut(s) 151
AccII CGCG 4 cut(s) 23, 117, 119, 206
AciI CCGC 6 cut(s) 136, 151, 386, 406, 450, 459
AclWI GGATC 1 cut(s) 161
AcuI CTGAAG 2 cut(s) 408, 429
AcyI GRCGYC 1 cut(s) 390
AfaI GTAC 6 cut(s) 122, 257, 317, 337, 455, 530
AfiI CCNNNNNNNGG 3 cut(s) 145, 449, 604
AflIII ACRYGT 1 cut(s) 252
AgsI TTSAA 1 cut(s) 708
AjiI CACGTC 2 cut(s) 196, 280
AjnI CCWGG 1 cut(s) 30
AluBI AGCT 6 cut(s) 28, 69, 75, 211, 356, 383
AluI AGCT 6 cut(s) 28, 69, 75, 211, 356, 383
Alw21I GWGCWC 1 cut(s) 99
Alw26I GTCTC 1 cut(s) 414
AlwI GGATC 1 cut(s) 161
Ama87I CYCGRG 1 cut(s) 105
AoxI GGCC 3 cut(s) 44, 169, 349
ApeKI GCWGC 5 cut(s) 39, 66, 208, 356, 383
AspLEI GCGC 4 cut(s) 66, 119, 208, 392
AspS9I GGNCC 3 cut(s) 52, 170, 349
AsuC2I CCSGG 1 cut(s) 270
AsuHPI GGTGA 5 cut(s) 122, 318, 410, 646, 673
AvaI CYCGRG 1 cut(s) 105
AvaII GGWCC 1 cut(s) 52
BanI GGYRCC 1 cut(s) 389
BarI GAAGNNNNNNTAC 2 cut(s) 113, 145
Bbv12I GWGCWC 1 cut(s) 99
BbvI GCAGC 5 cut(s) 51, 78, 220, 343, 370
BccI CCATC 5 cut(s) 64, 79, 182, 254, 490
BceAI ACGGC 1 cut(s) 184
BcgI CGANNNNNNTGC 4 cut(s) 302, 336, 482, 516
BciT130I CCWGG 1 cut(s) 32
BcnI CCSGG 1 cut(s) 270
BcoDI GTCTC 1 cut(s) 414
BfmI CTRYAG 2 cut(s) 70, 285
BfoI RGCGCY 1 cut(s) 393
BfuAI ACCTGC 1 cut(s) 278
BisI GCNGC 5 cut(s) 40, 67, 209, 357, 384
BlsI GCNGC 5 cut(s) 41, 68, 210, 358, 385
Bme1390I CCNGG 2 cut(s) 32, 270
Bme18I GGWCC 1 cut(s) 52
BmeT110I CYCGRG 1 cut(s) 105
BmgBI CACGTC 2 cut(s) 196, 280
BmgT120I GGNCC 3 cut(s) 52, 170, 349
BmiI GGNNCC 4 cut(s) 53, 54, 172, 391
BmrFI CCNGG 2 cut(s) 32, 270
BmsI GCATC 4 cut(s) 171, 300, 368, 658
BplI GAGNNNNNCTC 4 cut(s) 86, 118, 394, 426
BpuEI CTTGAG 1 cut(s) 128
BpuMI CCSGG 1 cut(s) 270
BsaHI GRCGYC 1 cut(s) 390
BsaI GGTCTC 1 cut(s) 414
BsaJI CCNNGG 4 cut(s) 47, 106, 268, 393
BsaXI ACNNNNNCTCC 4 cut(s) 116, 146, 173, 203
Bsc4I CCNNNNNNNGG 3 cut(s) 145, 449, 604
Bse1I ACTGG 1 cut(s) 551
BseBI CCWGG 1 cut(s) 32
BseDI CCNNGG 4 cut(s) 47, 106, 268, 393
BseGI GGATG 1 cut(s) 246
BseLI CCNNNNNNNGG 3 cut(s) 145, 449, 604
BseNI ACTGG 1 cut(s) 551
BseRI GAGGAG 2 cut(s) 91, 131
BseXI GCAGC 5 cut(s) 51, 78, 220, 343, 370
Bsh1236I CGCG 4 cut(s) 23, 117, 119, 206
BshFI GGCC 3 cut(s) 46, 171, 351
BshNI GGYRCC 1 cut(s) 389
BsiHKAI GWGCWC 1 cut(s) 99
BsiHKCI CYCGRG 1 cut(s) 105
BsiSI CCGG 1 cut(s) 270
BsiWI CGTACG 1 cut(s) 120
BslFI GGGAC 1 cut(s) 65
BslI CCNNNNNNNGG 3 cut(s) 145, 449, 604
BsmAI GTCTC 1 cut(s) 414
BsmFI GGGAC 1 cut(s) 65
BsmI GAATGC 1 cut(s) 614
BsnI GGCC 3 cut(s) 46, 171, 351
Bso31I GGTCTC 1 cut(s) 414
BsoBI CYCGRG 1 cut(s) 105
Bsp1286I GDGCHC 1 cut(s) 99
Bsp143I GATC 1 cut(s) 153
Bsp19I CCATGG 1 cut(s) 47
BspACI CCGC 6 cut(s) 136, 151, 386, 406, 450, 459
BspANI GGCC 3 cut(s) 46, 171, 351
BspFNI CGCG 4 cut(s) 23, 117, 119, 206
BspLI GGNNCC 4 cut(s) 53, 54, 172, 391
BspMAI CTGCAG 1 cut(s) 289
BspMI ACCTGC 1 cut(s) 278
BspPI GGATC 1 cut(s) 161
BspT107I GGYRCC 1 cut(s) 389
BspTNI GGTCTC 1 cut(s) 414
BsrBI CCGCTC 1 cut(s) 151
BsrI ACTGG 1 cut(s) 551
BssECI CCNNGG 4 cut(s) 47, 106, 268, 393
BssMI GATC 1 cut(s) 153
BssNI GRCGYC 1 cut(s) 390
BssT1I CCWWGG 2 cut(s) 47, 393
Bst2UI CCWGG 1 cut(s) 32
Bst6I CTCTTC 3 cut(s) 221, 378, 597
BstACI GRCGYC 1 cut(s) 390
BstAPI GCANNNNNTGC 1 cut(s) 293
BstC8I GCNNGC 3 cut(s) 37, 44, 388
BstDEI CTNAG 1 cut(s) 379
BstDSI CCRYGG 1 cut(s) 47
BstEII GGTNACC 1 cut(s) 324
BstENI CCTNNNNNAGG 1 cut(s) 143
BstF5I GGATG 1 cut(s) 246
BstFNI CGCG 4 cut(s) 23, 117, 119, 206
BstH2I RGCGCY 1 cut(s) 393
BstHHI GCGC 4 cut(s) 66, 119, 208, 392
BstKTI GATC 1 cut(s) 156
BstMAI GTCTC 1 cut(s) 414
BstMBI GATC 1 cut(s) 153
BstMWI GCNNNNNNNGC 7 cut(s) 72, 168, 293, 365, 389, 456, 544
BstNI CCWGG 1 cut(s) 32
BstNSI RCATGY 1 cut(s) 256
BstPI GGTNACC 1 cut(s) 324
BstSCI CCNGG 2 cut(s) 30, 268
BstSFI CTRYAG 2 cut(s) 70, 285
BstUI CGCG 4 cut(s) 23, 117, 119, 206
BstV1I GCAGC 5 cut(s) 51, 78, 220, 343, 370
BstXI CCANNNNNNTGG 1 cut(s) 346
BsuRI GGCC 3 cut(s) 46, 171, 351
BtgI CCRYGG 1 cut(s) 47
BtrI CACGTC 2 cut(s) 196, 280
BtsCI GGATG 1 cut(s) 246
BtsI GCAGTG 1 cut(s) 282
BtsIMutI CAGTG 1 cut(s) 282
BveI ACCTGC 1 cut(s) 278
Cac8I GCNNGC 3 cut(s) 37, 44, 388
CfoI GCGC 4 cut(s) 66, 119, 208, 392
Cfr13I GGNCC 3 cut(s) 52, 170, 349
Csp6I GTAC 6 cut(s) 121, 256, 316, 336, 454, 529
CviAII CATG 2 cut(s) 48, 253
CviQI GTAC 6 cut(s) 121, 256, 316, 336, 454, 529
DdeI CTNAG 1 cut(s) 379
DinI GGCGCC 1 cut(s) 391
DpnI GATC 1 cut(s) 155
DpnII GATC 1 cut(s) 153
Eam1104I CTCTTC 3 cut(s) 221, 378, 597
EarI CTCTTC 3 cut(s) 221, 378, 597
Eco130I CCWWGG 2 cut(s) 47, 393
Eco31I GGTCTC 1 cut(s) 414
Eco47I GGWCC 1 cut(s) 52
Eco57I CTGAAG 2 cut(s) 408, 429
Eco88I CYCGRG 1 cut(s) 105
Eco91I GGTNACC 1 cut(s) 324
EcoNI CCTNNNNNAGG 1 cut(s) 143
EcoO109I RGGNCCY 1 cut(s) 52
EcoO65I GGTNACC 1 cut(s) 324
EcoRII CCWGG 1 cut(s) 30
EcoT14I CCWWGG 2 cut(s) 47, 393
EgeI GGCGCC 1 cut(s) 391
EheI GGCGCC 1 cut(s) 391
ErhI CCWWGG 2 cut(s) 47, 393
FaeI CATG 2 cut(s) 51, 256
FaiI YATR 8 cut(s) 49, 254, 303, 431, 476, 540, 575, 593
FaqI GGGAC 1 cut(s) 65
FatI CATG 2 cut(s) 47, 252
FauI CCCGC 3 cut(s) 143, 379, 443
Fnu4HI GCNGC 5 cut(s) 40, 67, 209, 357, 384
FokI GGATG 1 cut(s) 233
Fsp4HI GCNGC 5 cut(s) 40, 67, 209, 357, 384
FspI TGCGCA 1 cut(s) 65
GlaI GCGC 4 cut(s) 65, 118, 207, 391
GluI GCNGC 5 cut(s) 40, 67, 209, 357, 384
HaeII RGCGCY 1 cut(s) 393
HaeIII GGCC 3 cut(s) 46, 171, 351
HapII CCGG 1 cut(s) 270
HhaI GCGC 4 cut(s) 66, 119, 208, 392
Hin1I GRCGYC 1 cut(s) 390
Hin1II CATG 2 cut(s) 51, 256
Hin6I GCGC 4 cut(s) 64, 117, 206, 390
HinP1I GCGC 4 cut(s) 64, 117, 206, 390
HinfI GANTC 5 cut(s) 79, 224, 433, 512, 621
HpaII CCGG 1 cut(s) 270
HphI GGTGA 5 cut(s) 122, 318, 410, 646, 673
Hpy166II GTNNAC 1 cut(s) 113
Hpy188I TCNGA 3 cut(s) 84, 229, 620
Hpy188III TCNNGA 1 cut(s) 105
Hpy8I GTNNAC 1 cut(s) 113
Hpy99I CGWCG 4 cut(s) 185, 200, 203, 206
HpyAV CCTTC 2 cut(s) 453, 677
HpyCH4IV ACGT 2 cut(s) 195, 279
HpyCH4V TGCA 5 cut(s) 162, 287, 296, 313, 359
HpyF10VI GCNNNNNNNGC 7 cut(s) 72, 168, 293, 365, 389, 456, 544
HpyF3I CTNAG 1 cut(s) 379
HpySE526I ACGT 2 cut(s) 195, 279
Hsp92I GRCGYC 1 cut(s) 390
Hsp92II CATG 2 cut(s) 51, 256
HspAI GCGC 4 cut(s) 64, 117, 206, 390
KasI GGCGCC 1 cut(s) 389
KflI GGGWCCC 1 cut(s) 52
Kzo9I GATC 1 cut(s) 153
LmnI GCTCC 2 cut(s) 33, 148
Lsp1109I GCAGC 5 cut(s) 51, 78, 220, 343, 370
LweI GCATC 4 cut(s) 171, 300, 368, 658
MaeII ACGT 2 cut(s) 195, 279
MaeIII GTNAC 4 cut(s) 275, 280, 324, 661
MalI GATC 1 cut(s) 155
MbiI CCGCTC 1 cut(s) 151
MboI GATC 1 cut(s) 153
MboII GAAGA 5 cut(s) 121, 208, 365, 580, 614
MhlI GDGCHC 1 cut(s) 99
MluCI AATT 4 cut(s) 262, 297, 480, 586
Mly113I GGCGCC 1 cut(s) 390
MnlI CCTC 9 cut(s) 101, 112, 149, 152, 199, 269, 396, 598, 625
MseI TTAA 2 cut(s) 11, 519
MslI CAYNNNNRTG 1 cut(s) 251
MspA1I CMGCKG 1 cut(s) 459
MspI CCGG 1 cut(s) 270
MspR9I CCNGG 2 cut(s) 32, 270
Mva1269I GAATGC 1 cut(s) 614
MvaI CCWGG 1 cut(s) 32
MvnI CGCG 4 cut(s) 23, 117, 119, 206
MwoI GCNNNNNNNGC 7 cut(s) 72, 168, 293, 365, 389, 456, 544
NarI GGCGCC 1 cut(s) 390
NciI CCSGG 1 cut(s) 270
NcoI CCATGG 1 cut(s) 47
NdeII GATC 1 cut(s) 153
NlaIII CATG 2 cut(s) 51, 256
NlaIV GGNNCC 4 cut(s) 53, 54, 172, 391
NmuCI GTSAC 4 cut(s) 275, 280, 324, 661
NsbI TGCGCA 1 cut(s) 65
NspI RCATGY 1 cut(s) 256
PciI ACATGT 1 cut(s) 252
PctI GAATGC 1 cut(s) 614
PfeI GAWTC 5 cut(s) 79, 224, 433, 512, 621
Pfl23II CGTACG 1 cut(s) 120
PkrI GCNGC 5 cut(s) 41, 68, 210, 358, 385
PluTI GGCGCC 1 cut(s) 393
PpuMI RGGWCCY 1 cut(s) 52
PscI ACATGT 1 cut(s) 252
Psp5II RGGWCCY 1 cut(s) 52
Psp6I CCWGG 1 cut(s) 30
PspEI GGTNACC 1 cut(s) 324
PspGI CCWGG 1 cut(s) 30
PspLI CGTACG 1 cut(s) 120
PspN4I GGNNCC 4 cut(s) 53, 54, 172, 391
PspPI GGNCC 3 cut(s) 52, 170, 349
PspPPI RGGWCCY 1 cut(s) 52
PstI CTGCAG 1 cut(s) 289
RsaI GTAC 6 cut(s) 122, 257, 317, 337, 455, 530
RsaNI GTAC 6 cut(s) 121, 256, 316, 336, 454, 529
RseI CAYNNNNRTG 1 cut(s) 251
SaqAI TTAA 2 cut(s) 11, 519
SatI GCNGC 5 cut(s) 40, 67, 209, 357, 384
Sau3AI GATC 1 cut(s) 153
Sau96I GGNCC 3 cut(s) 52, 170, 349
ScrFI CCNGG 2 cut(s) 32, 270
SduI GDGCHC 1 cut(s) 99
SfaNI GCATC 4 cut(s) 171, 300, 368, 658
SfcI CTRYAG 2 cut(s) 70, 285
SfoI GGCGCC 1 cut(s) 391
SinI GGWCC 1 cut(s) 52
SmiMI CAYNNNNRTG 1 cut(s) 251
SmlI CTYRAG 1 cut(s) 143
SmoI CTYRAG 1 cut(s) 143
Sse9I AATT 4 cut(s) 262, 297, 480, 586
SsiI CCGC 6 cut(s) 136, 151, 386, 406, 450, 459
SspDI GGCGCC 1 cut(s) 389
StyD4I CCNGG 2 cut(s) 30, 268
StyI CCWWGG 2 cut(s) 47, 393
TaiI ACGT 2 cut(s) 198, 282
TaqI TCGA 1 cut(s) 533
TaqII GACCGA 1 cut(s) 312
TasI AATT 4 cut(s) 262, 297, 480, 586
TatI WGTACW 2 cut(s) 315, 528
TfiI GAWTC 5 cut(s) 79, 224, 433, 512, 621
Tru1I TTAA 2 cut(s) 11, 519
Tru9I TTAA 2 cut(s) 11, 519
TscAI CASTG 1 cut(s) 289
TseFI GTSAC 4 cut(s) 275, 280, 324, 661
TseI GCWGC 5 cut(s) 39, 66, 208, 356, 383
Tsp45I GTSAC 4 cut(s) 275, 280, 324, 661
TspDTI ATGAA 3 cut(s) 425, 657, 696
TspRI CASTG 1 cut(s) 289
VpaK11BI GGWCC 1 cut(s) 52
XagI CCTNNNNNAGG 1 cut(s) 143
XceI RCATGY 1 cut(s) 256
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.