RLG00000005640

ATP-dependent Clp protease proteolytic subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0014077
Physical Location & Seq
Reverse (-)
520759 .. 522925
2167 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005640

Sequence Viewer

Length: 744 bp
ATGATGAGGAGCCTAATCTCAGGCGCCAAGCTCGTCGCTACCAGCAGAGCGCGAGTTTCGAGTCCATGGGGAACGAGCACGACGACGTCTTCGCGGTGCTACAGTCTGATCCCAATGGTGATAGAGCACTCGTCTCGAGGTGAGCGAGCGTACGACATCTTCTCTCGCCTTCTCAAGGAGCGCATAGTCTGCATCAACGGTCCCATCAACGACGACACCGCCCATGTCGTCGTCGCCCAGCTTCTCTTTCTCGAGTCTGAGAACCCATCCAAGCCCATCAATATGTACCTCAATTCTCCTGGTGGCCATGTCACTGCAGGTCTTGCAATTTATGATACTATGCAGTACATTCGTTCCCCAATCAATACAATTTGTTTGGGTCAAGCTGCATCAATGGCTTCACTTCTCTTAGCTGCGGGTGCCAAGGGTGAGAGGCGGTCACTTCCTAATGCCACAATTATGATTCATCAGCCTTCAGGTGGGTACAGCGGACAAGCAAAAGATATGACAATTCACACAAAGCAGATTGTCCGTGTGTGGGATTCACTCAATGCATTGTATTCAAAGCATACAGGGCAGTCAAAAGAGATGATCGAGAAGAATATGGATAGGGATCACTTCATGACCGCAGTAGAGGCAAAGGAGTTTGGAATAATTGATGAAGTCATTGATGAAAGACCAATGGCTCTGGTCACTGATGCTGTTGGCAAAGAAGGCAAAGAAAAGGACAAGAGTTCAAACTAG

Protein Analysis

248

Amino Acids

27.07

Weight (kDa)

8.38

Isoelectric Point (pI)

34.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLP_protease PF00574 46 - 225 6.1e-82 Clp protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014077)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23140
fragaria_vesca FvH4_4g36920
malus_domestica MD13G1005300.v1.1 MD16G1002600.v1.1
prunus_persica Prupe.1G347500_v2.0.a1
pyrus_communis pycom13g00450 pycom16g00330
rosa_chinensis RchiOBHm_Chr4g0446681
rosa_laevigata RLG00000005640
rosa_multiflora Rmu_sc0001753.1_g000041
rosa_roxburghii Rroxscaffold_5G00387040
rosa_rugosa Rorug04G0373600
rosa_samantha Rh4AG435100 Rh4BG444100 Rh4CG461400 Rh4DG442900
rosa_wichuraiana Rw4G037200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 89
Acc36I ACCTGC 1 cut(s) 308
AccB1I GGYRCC 2 cut(s) 23, 419
AccII CGCG 2 cut(s) 52, 94
AciI CCGC 6 cut(s) 94, 219, 416, 436, 489, 627
AclWI GGATC 2 cut(s) 103, 621
AcoI YGGCCR 1 cut(s) 304
AcuI CTGAAG 1 cut(s) 459
AcyI GRCGYC 2 cut(s) 24, 86
AfaI GTAC 4 cut(s) 152, 287, 347, 485
AfiI CCNNNNNNNGG 3 cut(s) 175, 479, 538
AgsI TTSAA 2 cut(s) 564, 738
AjnI CCWGG 1 cut(s) 298
AluBI AGCT 4 cut(s) 31, 241, 386, 413
AluI AGCT 4 cut(s) 31, 241, 386, 413
Alw21I GWGCWC 2 cut(s) 80, 129
Alw26I GTCTC 1 cut(s) 138
AlwI GGATC 2 cut(s) 103, 621
Ama87I CYCGRG 2 cut(s) 135, 251
AoxI GGCC 1 cut(s) 304
ApeKI GCWGC 2 cut(s) 386, 413
ArsI GACNNNNNNTTYG 2 cut(s) 73, 105
AspLEI GCGC 3 cut(s) 26, 52, 183
AspS9I GGNCC 1 cut(s) 200
AsuHPI GGTGA 3 cut(s) 130, 152, 440
AvaI CYCGRG 2 cut(s) 135, 251
AvaII GGWCC 1 cut(s) 200
BalI TGGCCA 1 cut(s) 306
BanI GGYRCC 2 cut(s) 23, 419
BarI GAAGNNNNNNTAC 2 cut(s) 143, 175
BbsI GAAGAC 1 cut(s) 81
Bbv12I GWGCWC 2 cut(s) 80, 129
BbvI GCAGC 2 cut(s) 373, 400
BccI CCATC 3 cut(s) 212, 274, 284
BcgI CGANNNNNNTGC 4 cut(s) 116, 150, 332, 366
BciT130I CCWGG 1 cut(s) 300
BcoDI GTCTC 1 cut(s) 138
BfaI CTAG 1 cut(s) 742
BfmI CTRYAG 2 cut(s) 100, 315
BfoI RGCGCY 1 cut(s) 27
BfuAI ACCTGC 1 cut(s) 308
BisI GCNGC 2 cut(s) 387, 414
BlsI GCNGC 2 cut(s) 388, 415
Bme1390I CCNGG 1 cut(s) 300
Bme18I GGWCC 1 cut(s) 200
BmeT110I CYCGRG 2 cut(s) 135, 251
BmgT120I GGNCC 1 cut(s) 200
BmiI GGNNCC 4 cut(s) 11, 25, 202, 421
BmrFI CCNGG 1 cut(s) 300
BmsI GCATC 3 cut(s) 201, 398, 688
BpiI GAAGAC 1 cut(s) 81
BpuEI CTTGAG 1 cut(s) 158
BsaBI GATNNNNATC 1 cut(s) 612
BsaHI GRCGYC 2 cut(s) 24, 86
BsaJI CCNNGG 2 cut(s) 65, 423
Bsc4I CCNNNNNNNGG 3 cut(s) 175, 479, 538
Bse8I GATNNNNATC 1 cut(s) 612
BseBI CCWGG 1 cut(s) 300
BseDI CCNNGG 2 cut(s) 65, 423
BseGI GGATG 1 cut(s) 266
BseJI GATNNNNATC 1 cut(s) 612
BseLI CCNNNNNNNGG 3 cut(s) 175, 479, 538
BseMII CTCAG 2 cut(s) 33, 249
BseRI GAGGAG 1 cut(s) 22
BseXI GCAGC 2 cut(s) 373, 400
BseYI CCCAGC 1 cut(s) 237
Bsh1236I CGCG 2 cut(s) 52, 94
BshFI GGCC 1 cut(s) 306
BshNI GGYRCC 2 cut(s) 23, 419
BsiHKAI GWGCWC 2 cut(s) 80, 129
BsiHKCI CYCGRG 2 cut(s) 135, 251
BsiWI CGTACG 1 cut(s) 150
BslFI GGGAC 1 cut(s) 186
BslI CCNNNNNNNGG 3 cut(s) 175, 479, 538
BsmAI GTCTC 1 cut(s) 138
BsmBI CGTCTC 1 cut(s) 138
BsmFI GGGAC 1 cut(s) 186
BsnI GGCC 1 cut(s) 306
BsoBI CYCGRG 2 cut(s) 135, 251
Bsp1286I GDGCHC 2 cut(s) 80, 129
Bsp143I GATC 3 cut(s) 108, 591, 613
Bsp19I CCATGG 1 cut(s) 65
BspACI CCGC 6 cut(s) 94, 219, 416, 436, 489, 627
BspANI GGCC 1 cut(s) 306
BspCNI CTCAG 2 cut(s) 32, 250
BspFNI CGCG 2 cut(s) 52, 94
BspHI TCATGA 1 cut(s) 621
BspLI GGNNCC 4 cut(s) 11, 25, 202, 421
BspMAI CTGCAG 1 cut(s) 319
BspMI ACCTGC 1 cut(s) 308
BspPI GGATC 2 cut(s) 103, 621
BspT107I GGYRCC 2 cut(s) 23, 419
BssECI CCNNGG 2 cut(s) 65, 423
BssMI GATC 3 cut(s) 108, 591, 613
BssNI GRCGYC 2 cut(s) 24, 86
BssT1I CCWWGG 2 cut(s) 65, 423
Bst2UI CCWGG 1 cut(s) 300
Bst4CI ACNGT 2 cut(s) 104, 200
BstACI GRCGYC 2 cut(s) 24, 86
BstAPI GCANNNNNTGC 2 cut(s) 189, 323
BstC8I GCNNGC 1 cut(s) 147
BstDEI CTNAG 3 cut(s) 19, 258, 409
BstDSI CCRYGG 1 cut(s) 65
BstENI CCTNNNNNAGG 1 cut(s) 173
BstF5I GGATG 1 cut(s) 266
BstFNI CGCG 2 cut(s) 52, 94
BstH2I RGCGCY 1 cut(s) 27
BstHHI GCGC 3 cut(s) 26, 52, 183
BstKTI GATC 3 cut(s) 111, 594, 616
BstMAI GTCTC 1 cut(s) 138
BstMBI GATC 3 cut(s) 108, 591, 613
BstMWI GCNNNNNNNGC 7 cut(s) 189, 323, 395, 419, 574, 635, 714
BstNI CCWGG 1 cut(s) 300
BstSCI CCNGG 1 cut(s) 298
BstSFI CTRYAG 2 cut(s) 100, 315
BstUI CGCG 2 cut(s) 52, 94
BstV1I GCAGC 2 cut(s) 373, 400
BstV2I GAAGAC 1 cut(s) 81
BsuRI GGCC 1 cut(s) 306
BtgI CCRYGG 1 cut(s) 65
BtsCI GGATG 1 cut(s) 266
BtsI GCAGTG 1 cut(s) 312
BtsIMutI CAGTG 2 cut(s) 312, 693
BveI ACCTGC 1 cut(s) 308
Cac8I GCNNGC 1 cut(s) 147
CciI TCATGA 1 cut(s) 621
CfoI GCGC 3 cut(s) 26, 52, 183
Cfr13I GGNCC 1 cut(s) 200
Csp6I GTAC 4 cut(s) 151, 286, 346, 484
CviAII CATG 4 cut(s) 66, 224, 308, 622
CviQI GTAC 4 cut(s) 151, 286, 346, 484
DdeI CTNAG 3 cut(s) 19, 258, 409
DinI GGCGCC 1 cut(s) 25
DpnI GATC 3 cut(s) 110, 593, 615
DpnII GATC 3 cut(s) 108, 591, 613
EaeI YGGCCR 1 cut(s) 304
Eco130I CCWWGG 2 cut(s) 65, 423
Eco47I GGWCC 1 cut(s) 200
Eco57I CTGAAG 1 cut(s) 459
Eco88I CYCGRG 2 cut(s) 135, 251
EcoNI CCTNNNNNAGG 1 cut(s) 173
EcoRII CCWGG 1 cut(s) 298
EcoT14I CCWWGG 2 cut(s) 65, 423
EcoT22I ATGCAT 1 cut(s) 556
EgeI GGCGCC 1 cut(s) 25
EheI GGCGCC 1 cut(s) 25
ErhI CCWWGG 2 cut(s) 65, 423
Esp3I CGTCTC 1 cut(s) 138
FaeI CATG 4 cut(s) 69, 227, 311, 625
FaqI GGGAC 1 cut(s) 186
FatI CATG 4 cut(s) 65, 223, 307, 621
FauI CCCGC 1 cut(s) 409
Fnu4HI GCNGC 2 cut(s) 387, 414
FokI GGATG 1 cut(s) 253
Fsp4HI GCNGC 2 cut(s) 387, 414
FspBI CTAG 1 cut(s) 742
GlaI GCGC 3 cut(s) 25, 51, 182
GluI GCNGC 2 cut(s) 387, 414
GsaI CCCAGC 1 cut(s) 241
HaeII RGCGCY 1 cut(s) 27
HaeIII GGCC 1 cut(s) 306
HhaI GCGC 3 cut(s) 26, 52, 183
Hin1I GRCGYC 2 cut(s) 24, 86
Hin1II CATG 4 cut(s) 69, 227, 311, 625
Hin6I GCGC 3 cut(s) 24, 50, 181
HinP1I GCGC 3 cut(s) 24, 50, 181
HinfI GANTC 4 cut(s) 61, 254, 463, 542
HphI GGTGA 3 cut(s) 130, 152, 440
Hpy188I TCNGA 2 cut(s) 108, 259
Hpy188III TCNNGA 4 cut(s) 135, 251, 595, 622
Hpy99I CGWCG 6 cut(s) 38, 85, 88, 215, 233, 236
HpyAV CCTTC 3 cut(s) 179, 483, 707
HpyCH4III ACNGT 2 cut(s) 104, 200
HpyCH4IV ACGT 1 cut(s) 86
HpyCH4V TGCA 6 cut(s) 192, 317, 326, 343, 389, 554
HpyF10VI GCNNNNNNNGC 7 cut(s) 189, 323, 395, 419, 574, 635, 714
HpyF3I CTNAG 3 cut(s) 19, 258, 409
HpySE526I ACGT 1 cut(s) 86
Hsp92I GRCGYC 2 cut(s) 24, 86
Hsp92II CATG 4 cut(s) 69, 227, 311, 625
HspAI GCGC 3 cut(s) 24, 50, 181
KasI GGCGCC 1 cut(s) 23
Kzo9I GATC 3 cut(s) 108, 591, 613
LmnI GCTCC 2 cut(s) 9, 178
LpnPI CCDG 9 cut(s) 6, 55, 251, 285, 303, 312, 462, 558, 674
Lsp1109I GCAGC 2 cut(s) 373, 400
LweI GCATC 3 cut(s) 201, 398, 688
MaeI CTAG 1 cut(s) 742
MaeII ACGT 1 cut(s) 86
MaeIII GTNAC 3 cut(s) 310, 438, 691
MalI GATC 3 cut(s) 110, 593, 615
MboI GATC 3 cut(s) 108, 591, 613
MboII GAAGA 3 cut(s) 81, 151, 610
MhlI GDGCHC 2 cut(s) 80, 129
MlsI TGGCCA 1 cut(s) 306
MluCI AATT 6 cut(s) 292, 327, 369, 456, 510, 654
MluNI TGGCCA 1 cut(s) 306
Mly113I GGCGCC 1 cut(s) 24
MlyI GAGTC 2 cut(s) 70, 263
MnlI CCTC 4 cut(s) 131, 299, 426, 628
Mox20I TGGCCA 1 cut(s) 306
Mph1103I ATGCAT 1 cut(s) 556
MscI TGGCCA 1 cut(s) 306
MslI CAYNNNNRTG 2 cut(s) 281, 458
Msp20I TGGCCA 1 cut(s) 306
MspA1I CMGCKG 1 cut(s) 489
MspR9I CCNGG 1 cut(s) 300
MvaI CCWGG 1 cut(s) 300
MvnI CGCG 2 cut(s) 52, 94
MwoI GCNNNNNNNGC 7 cut(s) 189, 323, 395, 419, 574, 635, 714
NarI GGCGCC 1 cut(s) 24
NcoI CCATGG 1 cut(s) 65
NdeII GATC 3 cut(s) 108, 591, 613
NlaIII CATG 4 cut(s) 69, 227, 311, 625
NlaIV GGNNCC 4 cut(s) 11, 25, 202, 421
NmuCI GTSAC 3 cut(s) 310, 438, 691
NsiI ATGCAT 1 cut(s) 556
PaeR7I CTCGAG 2 cut(s) 135, 251
PagI TCATGA 1 cut(s) 621
PcsI WCGNNNNNNNCGW 2 cut(s) 80, 142
PfeI GAWTC 2 cut(s) 463, 542
Pfl23II CGTACG 1 cut(s) 150
PflFI GACNNNGTC 1 cut(s) 85
PkrI GCNGC 2 cut(s) 388, 415
PleI GAGTC 2 cut(s) 69, 262
PluTI GGCGCC 1 cut(s) 27
PpsI GAGTC 2 cut(s) 69, 262
Psp6I CCWGG 1 cut(s) 298
PspFI CCCAGC 1 cut(s) 237
PspGI CCWGG 1 cut(s) 298
PspLI CGTACG 1 cut(s) 150
PspN4I GGNNCC 4 cut(s) 11, 25, 202, 421
PspPI GGNCC 1 cut(s) 200
PstI CTGCAG 1 cut(s) 319
PsyI GACNNNGTC 1 cut(s) 85
RsaI GTAC 4 cut(s) 152, 287, 347, 485
RsaNI GTAC 4 cut(s) 151, 286, 346, 484
RseI CAYNNNNRTG 2 cut(s) 281, 458
SatI GCNGC 2 cut(s) 387, 414
Sau3AI GATC 3 cut(s) 108, 591, 613
Sau96I GGNCC 1 cut(s) 200
SchI GAGTC 2 cut(s) 70, 263
ScrFI CCNGG 1 cut(s) 300
SduI GDGCHC 2 cut(s) 80, 129
SetI ASST 9 cut(s) 33, 89, 142, 243, 291, 322, 388, 415, 481
SfaNI GCATC 3 cut(s) 201, 398, 688
SfcI CTRYAG 2 cut(s) 100, 315
SfoI GGCGCC 1 cut(s) 25
Sfr274I CTCGAG 2 cut(s) 135, 251
SinI GGWCC 1 cut(s) 200
SlaI CTCGAG 2 cut(s) 135, 251
SmiMI CAYNNNNRTG 2 cut(s) 281, 458
SmlI CTYRAG 3 cut(s) 135, 173, 251
SmoI CTYRAG 3 cut(s) 135, 173, 251
Sse9I AATT 6 cut(s) 292, 327, 369, 456, 510, 654
SsiI CCGC 6 cut(s) 94, 219, 416, 436, 489, 627
SspDI GGCGCC 1 cut(s) 23
SspMI CTAG 1 cut(s) 742
StyD4I CCNGG 1 cut(s) 298
StyI CCWWGG 2 cut(s) 65, 423
TaaI ACNGT 2 cut(s) 104, 200
TaiI ACGT 1 cut(s) 89
TaqI TCGA 4 cut(s) 59, 136, 252, 594
TasI AATT 6 cut(s) 292, 327, 369, 456, 510, 654
TatI WGTACW 1 cut(s) 345
TfiI GAWTC 2 cut(s) 463, 542
TscAI CASTG 2 cut(s) 319, 700
TseFI GTSAC 3 cut(s) 310, 438, 691
TseI GCWGC 2 cut(s) 386, 413
Tsp45I GTSAC 3 cut(s) 310, 438, 691
TspDTI ATGAA 4 cut(s) 455, 610, 675, 687
TspGWI ACGGA 1 cut(s) 521
TspRI CASTG 2 cut(s) 319, 700
Tth111I GACNNNGTC 1 cut(s) 85
VpaK11BI GGWCC 1 cut(s) 200
XagI CCTNNNNNAGG 1 cut(s) 173
XhoI CTCGAG 2 cut(s) 135, 251
XspI CTAG 1 cut(s) 742
ZraI GACGTC 1 cut(s) 87
Zsp2I ATGCAT 1 cut(s) 556
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.