MD13G1005300.v1.1

ATP-dependent Clp protease proteolytic subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0014077
Physical Location & Seq
Forward (+)
336369 .. 336750
382 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1005300.v1.1.491

Sequence Viewer

Length: 360 bp
ATGAGAGGCTTAATCTCAAAGGCGAAGCTCCTGGCAAGTAGCAGGCCATGGCGCCCATCTCTGCGCAGCTACAGCTTGATCCCCATGGTCATTGAGCACTCCTCCCGAGGGGAACGCGCGTACGACATCTTCTCCCGCCTCCTTAAGGAGCGGATCATTTGCATCAACGGACCCATCGACGACGACACTTCCCACGTCGTCGTCGCGCAGCTTCTCTACTTGGAATCCGAGAACCCGTCGAAGCCCATCCACATGTACCTTAATTCCCCCGGTGGTCACGTCACTGCAGGTCCTTTTGTTATTTCCCATTTTTATTTCTGTTTAATTTTTTATAGGTTGTGTGAAATTCTCGTGCTTTAA

Protein Analysis

120

Amino Acids

13.58

Weight (kDa)

8.88

Isoelectric Point (pI)

46.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLP_protease PF00574 35 - 99 2.1e-22 Clp protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014077)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23140
fragaria_vesca FvH4_4g36920
malus_domestica MD13G1005300.v1.1 MD16G1002600.v1.1
prunus_persica Prupe.1G347500_v2.0.a1
pyrus_communis pycom13g00450 pycom16g00330
rosa_chinensis RchiOBHm_Chr4g0446681
rosa_laevigata RLG00000005640
rosa_multiflora Rmu_sc0001753.1_g000041
rosa_roxburghii Rroxscaffold_5G00387040
rosa_rugosa Rorug04G0373600
rosa_samantha Rh4AG435100 Rh4BG444100 Rh4CG461400 Rh4DG442900
rosa_wichuraiana Rw4G037200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 65
Acc36I ACCTGC 1 cut(s) 278
AccB1I GGYRCC 1 cut(s) 51
AccBSI CCGCTC 1 cut(s) 151
AccII CGCG 3 cut(s) 117, 119, 206
AciI CCGC 2 cut(s) 136, 151
AclWI GGATC 2 cut(s) 73, 161
AcsI RAATTY 1 cut(s) 345
AcyI GRCGYC 1 cut(s) 52
AfaI GTAC 2 cut(s) 122, 257
AfiI CCNNNNNNNGG 2 cut(s) 108, 145
AflII CTTAAG 1 cut(s) 143
AflIII ACRYGT 1 cut(s) 252
AjiI CACGTC 2 cut(s) 196, 280
AjnI CCWGG 1 cut(s) 30
AluBI AGCT 4 cut(s) 28, 69, 75, 211
AluI AGCT 4 cut(s) 28, 69, 75, 211
Alw21I GWGCWC 1 cut(s) 99
AlwI GGATC 2 cut(s) 73, 161
Ama87I CYCGRG 1 cut(s) 105
AoxI GGCC 1 cut(s) 44
ApeKI GCWGC 2 cut(s) 66, 208
ApoI RAATTY 1 cut(s) 345
AspLEI GCGC 4 cut(s) 54, 66, 119, 208
AspS9I GGNCC 2 cut(s) 170, 290
AsuC2I CCSGG 1 cut(s) 270
AvaI CYCGRG 1 cut(s) 105
AvaII GGWCC 2 cut(s) 170, 290
BanI GGYRCC 1 cut(s) 51
BarI GAAGNNNNNNTAC 2 cut(s) 113, 145
BauI CACGAG 1 cut(s) 350
Bbv12I GWGCWC 1 cut(s) 99
BbvI GCAGC 2 cut(s) 78, 220
BccI CCATC 3 cut(s) 64, 182, 254
BciT130I CCWGG 1 cut(s) 32
BcnI CCSGG 1 cut(s) 270
BfmI CTRYAG 2 cut(s) 70, 285
BfoI RGCGCY 1 cut(s) 55
BfrI CTTAAG 1 cut(s) 143
BfuAI ACCTGC 1 cut(s) 278
BisI GCNGC 2 cut(s) 67, 209
BlsI GCNGC 2 cut(s) 68, 210
Bme1390I CCNGG 2 cut(s) 32, 270
Bme18I GGWCC 2 cut(s) 170, 290
BmeT110I CYCGRG 1 cut(s) 105
BmgBI CACGTC 2 cut(s) 196, 280
BmgT120I GGNCC 2 cut(s) 170, 290
BmiI GGNNCC 2 cut(s) 53, 172
BmrFI CCNGG 2 cut(s) 32, 270
BmsI GCATC 1 cut(s) 171
BplI GAGNNNNNCTC 2 cut(s) 86, 118
BpuMI CCSGG 1 cut(s) 270
BsaHI GRCGYC 1 cut(s) 52
BsaJI CCNNGG 4 cut(s) 47, 84, 106, 268
BsaXI ACNNNNNCTCC 2 cut(s) 116, 146
Bsc4I CCNNNNNNNGG 2 cut(s) 108, 145
BseBI CCWGG 1 cut(s) 32
BseDI CCNNGG 4 cut(s) 47, 84, 106, 268
BseGI GGATG 1 cut(s) 246
BseLI CCNNNNNNNGG 2 cut(s) 108, 145
BseRI GAGGAG 1 cut(s) 91
BseXI GCAGC 2 cut(s) 78, 220
Bsh1236I CGCG 3 cut(s) 117, 119, 206
BshFI GGCC 1 cut(s) 46
BshNI GGYRCC 1 cut(s) 51
BsiHKAI GWGCWC 1 cut(s) 99
BsiHKCI CYCGRG 1 cut(s) 105
BsiSI CCGG 1 cut(s) 270
BsiWI CGTACG 1 cut(s) 120
BslI CCNNNNNNNGG 2 cut(s) 108, 145
BsnI GGCC 1 cut(s) 46
BsoBI CYCGRG 1 cut(s) 105
Bsp1286I GDGCHC 1 cut(s) 99
Bsp143I GATC 2 cut(s) 78, 153
Bsp19I CCATGG 2 cut(s) 47, 84
BspACI CCGC 2 cut(s) 136, 151
BspANI GGCC 1 cut(s) 46
BspFNI CGCG 3 cut(s) 117, 119, 206
BspLI GGNNCC 2 cut(s) 53, 172
BspMAI CTGCAG 1 cut(s) 289
BspMI ACCTGC 1 cut(s) 278
BspPI GGATC 2 cut(s) 73, 161
BspT107I GGYRCC 1 cut(s) 51
BspTI CTTAAG 1 cut(s) 143
BsrBI CCGCTC 1 cut(s) 151
BssECI CCNNGG 4 cut(s) 47, 84, 106, 268
BssMI GATC 2 cut(s) 78, 153
BssNI GRCGYC 1 cut(s) 52
BssSI CACGAG 1 cut(s) 350
BssT1I CCWWGG 2 cut(s) 47, 84
Bst2BI CACGAG 1 cut(s) 350
Bst2UI CCWGG 1 cut(s) 32
BstACI GRCGYC 1 cut(s) 52
BstAFI CTTAAG 1 cut(s) 143
BstC8I GCNNGC 1 cut(s) 44
BstDSI CCRYGG 2 cut(s) 47, 84
BstENI CCTNNNNNAGG 1 cut(s) 143
BstF5I GGATG 1 cut(s) 246
BstFNI CGCG 3 cut(s) 117, 119, 206
BstH2I RGCGCY 1 cut(s) 55
BstHHI GCGC 4 cut(s) 54, 66, 119, 208
BstKTI GATC 2 cut(s) 81, 156
BstMBI GATC 2 cut(s) 78, 153
BstMWI GCNNNNNNNGC 1 cut(s) 72
BstNI CCWGG 1 cut(s) 32
BstNSI RCATGY 1 cut(s) 256
BstSCI CCNGG 2 cut(s) 30, 268
BstSFI CTRYAG 2 cut(s) 70, 285
BstUI CGCG 3 cut(s) 117, 119, 206
BstV1I GCAGC 2 cut(s) 78, 220
BsuRI GGCC 1 cut(s) 46
BtgI CCRYGG 2 cut(s) 47, 84
BtrI CACGTC 2 cut(s) 196, 280
BtsCI GGATG 1 cut(s) 246
BtsI GCAGTG 1 cut(s) 282
BtsIMutI CAGTG 1 cut(s) 282
BveI ACCTGC 1 cut(s) 278
Cac8I GCNNGC 1 cut(s) 44
CfoI GCGC 4 cut(s) 54, 66, 119, 208
Cfr13I GGNCC 2 cut(s) 170, 290
Csp6I GTAC 2 cut(s) 121, 256
CviAII CATG 3 cut(s) 48, 85, 253
CviJI RGCY 7 cut(s) 9, 28, 46, 69, 75, 211, 244
CviKI_1 RGCY 7 cut(s) 9, 28, 46, 69, 75, 211, 244
CviQI GTAC 2 cut(s) 121, 256
DinI GGCGCC 1 cut(s) 53
DpnI GATC 2 cut(s) 80, 155
DpnII GATC 2 cut(s) 78, 153
Eco130I CCWWGG 2 cut(s) 47, 84
Eco47I GGWCC 2 cut(s) 170, 290
Eco88I CYCGRG 1 cut(s) 105
EcoNI CCTNNNNNAGG 1 cut(s) 143
EcoO109I RGGNCCY 1 cut(s) 290
EcoRII CCWGG 1 cut(s) 30
EcoT14I CCWWGG 2 cut(s) 47, 84
EgeI GGCGCC 1 cut(s) 53
EheI GGCGCC 1 cut(s) 53
ErhI CCWWGG 2 cut(s) 47, 84
FaeI CATG 3 cut(s) 51, 88, 256
FaiI YATR 4 cut(s) 49, 86, 254, 333
FatI CATG 3 cut(s) 47, 84, 252
FauI CCCGC 1 cut(s) 143
Fnu4HI GCNGC 2 cut(s) 67, 209
FokI GGATG 1 cut(s) 233
Fsp4HI GCNGC 2 cut(s) 67, 209
FspI TGCGCA 1 cut(s) 65
GlaI GCGC 4 cut(s) 53, 65, 118, 207
GluI GCNGC 2 cut(s) 67, 209
HaeII RGCGCY 1 cut(s) 55
HaeIII GGCC 1 cut(s) 46
HapII CCGG 1 cut(s) 270
HhaI GCGC 4 cut(s) 54, 66, 119, 208
Hin1I GRCGYC 1 cut(s) 52
Hin1II CATG 3 cut(s) 51, 88, 256
Hin6I GCGC 4 cut(s) 52, 64, 117, 206
HinP1I GCGC 4 cut(s) 52, 64, 117, 206
HinfI GANTC 1 cut(s) 224
HpaII CCGG 1 cut(s) 270
Hpy188I TCNGA 1 cut(s) 229
Hpy188III TCNNGA 1 cut(s) 105
Hpy99I CGWCG 6 cut(s) 182, 185, 200, 203, 206, 241
HpyCH4IV ACGT 2 cut(s) 195, 279
HpyCH4V TGCA 2 cut(s) 162, 287
HpyF10VI GCNNNNNNNGC 1 cut(s) 72
HpySE526I ACGT 2 cut(s) 195, 279
Hsp92I GRCGYC 1 cut(s) 52
Hsp92II CATG 3 cut(s) 51, 88, 256
HspAI GCGC 4 cut(s) 52, 64, 117, 206
KasI GGCGCC 1 cut(s) 51
Kzo9I GATC 2 cut(s) 78, 153
LmnI GCTCC 2 cut(s) 33, 148
LpnPI CCDG 5 cut(s) 17, 28, 44, 273, 283
Lsp1109I GCAGC 2 cut(s) 78, 220
LweI GCATC 1 cut(s) 171
MaeII ACGT 2 cut(s) 195, 279
MaeIII GTNAC 2 cut(s) 275, 280
MalI GATC 2 cut(s) 80, 155
MbiI CCGCTC 1 cut(s) 151
MboI GATC 2 cut(s) 78, 153
MboII GAAGA 1 cut(s) 121
MhlI GDGCHC 1 cut(s) 99
MluCI AATT 3 cut(s) 262, 324, 345
Mly113I GGCGCC 1 cut(s) 52
MnlI CCTC 3 cut(s) 101, 112, 149
MseI TTAA 5 cut(s) 11, 144, 261, 323, 358
MslI CAYNNNNRTG 1 cut(s) 251
MspCI CTTAAG 1 cut(s) 143
MspI CCGG 1 cut(s) 270
MspR9I CCNGG 2 cut(s) 32, 270
MvaI CCWGG 1 cut(s) 32
MvnI CGCG 3 cut(s) 117, 119, 206
MwoI GCNNNNNNNGC 1 cut(s) 72
NarI GGCGCC 1 cut(s) 52
NciI CCSGG 1 cut(s) 270
NcoI CCATGG 2 cut(s) 47, 84
NdeII GATC 2 cut(s) 78, 153
NlaIII CATG 3 cut(s) 51, 88, 256
NlaIV GGNNCC 2 cut(s) 53, 172
NmuCI GTSAC 2 cut(s) 275, 280
NsbI TGCGCA 1 cut(s) 65
NspI RCATGY 1 cut(s) 256
PciI ACATGT 1 cut(s) 252
PcsI WCGNNNNNNNCGW 1 cut(s) 174
PfeI GAWTC 1 cut(s) 224
Pfl23II CGTACG 1 cut(s) 120
PkrI GCNGC 2 cut(s) 68, 210
PluTI GGCGCC 1 cut(s) 55
PpuMI RGGWCCY 1 cut(s) 290
PscI ACATGT 1 cut(s) 252
Psp5II RGGWCCY 1 cut(s) 290
Psp6I CCWGG 1 cut(s) 30
PspGI CCWGG 1 cut(s) 30
PspLI CGTACG 1 cut(s) 120
PspN4I GGNNCC 2 cut(s) 53, 172
PspPI GGNCC 2 cut(s) 170, 290
PspPPI RGGWCCY 1 cut(s) 290
PstI CTGCAG 1 cut(s) 289
RsaI GTAC 2 cut(s) 122, 257
RsaNI GTAC 2 cut(s) 121, 256
RseI CAYNNNNRTG 1 cut(s) 251
SaqAI TTAA 5 cut(s) 11, 144, 261, 323, 358
SatI GCNGC 2 cut(s) 67, 209
Sau3AI GATC 2 cut(s) 78, 153
Sau96I GGNCC 2 cut(s) 170, 290
ScrFI CCNGG 2 cut(s) 32, 270
SduI GDGCHC 1 cut(s) 99
SetI ASST 9 cut(s) 30, 71, 77, 198, 213, 261, 282, 292, 338
SfaNI GCATC 1 cut(s) 171
SfcI CTRYAG 2 cut(s) 70, 285
SfoI GGCGCC 1 cut(s) 53
SinI GGWCC 2 cut(s) 170, 290
SmiMI CAYNNNNRTG 1 cut(s) 251
SmlI CTYRAG 1 cut(s) 143
SmoI CTYRAG 1 cut(s) 143
Sse9I AATT 3 cut(s) 262, 324, 345
SsiI CCGC 2 cut(s) 136, 151
SspDI GGCGCC 1 cut(s) 51
StyD4I CCNGG 2 cut(s) 30, 268
StyI CCWWGG 2 cut(s) 47, 84
TaiI ACGT 2 cut(s) 198, 282
TaqI TCGA 2 cut(s) 177, 239
TasI AATT 3 cut(s) 262, 324, 345
TfiI GAWTC 1 cut(s) 224
Tru1I TTAA 5 cut(s) 11, 144, 261, 323, 358
Tru9I TTAA 5 cut(s) 11, 144, 261, 323, 358
TscAI CASTG 1 cut(s) 289
TseFI GTSAC 2 cut(s) 275, 280
TseI GCWGC 2 cut(s) 66, 208
Tsp45I GTSAC 2 cut(s) 275, 280
TspGWI ACGGA 1 cut(s) 183
TspRI CASTG 1 cut(s) 289
Vha464I CTTAAG 1 cut(s) 143
VpaK11BI GGWCC 2 cut(s) 170, 290
XagI CCTNNNNNAGG 1 cut(s) 143
XapI RAATTY 1 cut(s) 345
XceI RCATGY 1 cut(s) 256
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.