Rmu_sc0001753.1_g000041

ATP-dependent Clp protease proteolytic subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001753.1
OG0014077
Physical Location & Seq
Reverse (-)
195414 .. 197560
2147 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001753.1_g000041.1.cds

Sequence Viewer

Length: 747 bp
atgaggagcctaatctcaggcgccaagctcgtcgcgaccagcagaagagcacgagtttcgagtccatggggaacgagcccgacgacgacgtcttctcggtgctacagtctgatcccaatggtgatagagcactcgtctcgaggcgagcgagcgtacgacatcttctctcgccttctcaaggagcgcatagtctgcatcaacggtcccatcaacgacgacaccgcccatgtcgtcgtcgcccagcttctctttctcgagtctgagaacccatccaagcccatcaatatgtacctcaattctcctggtggccatgtcactgcaggtcttgcaatttatgatactatgcagtacattcggtcaccgatcaatacaatttgtttgggtcaagctgcatcaatggcttcacttctcttagctgcgggtgccaagggtgagaggcggtcacttcccaatgccacaattatgattcatcagccttcaggtgggtacagcggacaagcaaaagatatgacaattcacacaaagcagattgtccgtgtgtgggattcactcaatgcattgtattcaaagcatacagggcagtcaatagagatgatcgagaagaatatggatagggatcacttcatgactgcagtagaggcaaaggagtttggaataattgatgaagtcattgatgaaagaccaatggctctggtcactgatgctgttggcaaagaaggcaaagaaaaggacaagagttcaaactag

Protein Analysis

248

Amino Acids

27.18

Weight (kDa)

8.39

Isoelectric Point (pI)

40.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014077)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23140
fragaria_vesca FvH4_4g36920
malus_domestica MD13G1005300.v1.1 MD16G1002600.v1.1
prunus_persica Prupe.1G347500_v2.0.a1
pyrus_communis pycom13g00450 pycom16g00330
rosa_chinensis RchiOBHm_Chr4g0446681
rosa_laevigata RLG00000005640
rosa_multiflora Rmu_sc0001753.1_g000041
rosa_roxburghii Rroxscaffold_5G00387040
rosa_rugosa Rorug04G0373600
rosa_samantha Rh4AG435100 Rh4BG444100 Rh4CG461400 Rh4DG442900
rosa_wichuraiana Rw4G037200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 88
AatII GACGTC 1 cut(s) 92
Acc36I ACCTGC 1 cut(s) 311
AccB1I GGYRCC 2 cut(s) 20, 422
AccII CGCG 1 cut(s) 35
AciI CCGC 4 cut(s) 222, 419, 439, 492
AclWI GGATC 2 cut(s) 106, 624
AcoI YGGCCR 1 cut(s) 307
AcuI CTGAAG 1 cut(s) 462
AcyI GRCGYC 2 cut(s) 21, 89
AfaI GTAC 4 cut(s) 155, 290, 350, 488
AfiI CCNNNNNNNGG 3 cut(s) 178, 482, 541
AgsI TTSAA 2 cut(s) 567, 741
AjnI CCWGG 1 cut(s) 301
AluBI AGCT 4 cut(s) 28, 244, 389, 416
AluI AGCT 4 cut(s) 28, 244, 389, 416
Alw21I GWGCWC 2 cut(s) 52, 132
Alw26I GTCTC 1 cut(s) 141
AlwI GGATC 2 cut(s) 106, 624
Ama87I CYCGRG 2 cut(s) 138, 254
AoxI GGCC 1 cut(s) 307
ApeKI GCWGC 2 cut(s) 389, 416
AspLEI GCGC 2 cut(s) 23, 186
AspS9I GGNCC 1 cut(s) 203
AsuHPI GGTGA 3 cut(s) 133, 351, 443
AvaI CYCGRG 2 cut(s) 138, 254
AvaII GGWCC 1 cut(s) 203
BalI TGGCCA 1 cut(s) 309
BanI GGYRCC 2 cut(s) 20, 422
BanII GRGCYC 1 cut(s) 80
BarI GAAGNNNNNNTAC 2 cut(s) 146, 178
BauI CACGAG 1 cut(s) 51
BbsI GAAGAC 1 cut(s) 84
Bbv12I GWGCWC 2 cut(s) 52, 132
BbvI GCAGC 2 cut(s) 376, 403
BccI CCATC 3 cut(s) 215, 277, 287
BcgI CGANNNNNNTGC 6 cut(s) 39, 73, 119, 153, 335, 369
BciT130I CCWGG 1 cut(s) 303
BcoDI GTCTC 1 cut(s) 141
BfaI CTAG 1 cut(s) 745
BfmI CTRYAG 3 cut(s) 103, 318, 630
BfoI RGCGCY 1 cut(s) 24
BfuAI ACCTGC 1 cut(s) 311
BisI GCNGC 2 cut(s) 390, 417
BlsI GCNGC 2 cut(s) 391, 418
Bme1390I CCNGG 1 cut(s) 303
Bme18I GGWCC 1 cut(s) 203
BmeT110I CYCGRG 2 cut(s) 138, 254
BmgT120I GGNCC 1 cut(s) 203
BmiI GGNNCC 4 cut(s) 8, 22, 205, 424
BmrFI CCNGG 1 cut(s) 303
BmsI GCATC 3 cut(s) 204, 401, 691
BpiI GAAGAC 1 cut(s) 84
BpuEI CTTGAG 1 cut(s) 161
BsaBI GATNNNNATC 1 cut(s) 615
BsaHI GRCGYC 2 cut(s) 21, 89
BsaJI CCNNGG 2 cut(s) 65, 426
Bsc4I CCNNNNNNNGG 3 cut(s) 178, 482, 541
Bse8I GATNNNNATC 1 cut(s) 615
BseBI CCWGG 1 cut(s) 303
BseDI CCNNGG 2 cut(s) 65, 426
BseGI GGATG 1 cut(s) 269
BseJI GATNNNNATC 1 cut(s) 615
BseLI CCNNNNNNNGG 3 cut(s) 178, 482, 541
BseMII CTCAG 2 cut(s) 30, 252
BseRI GAGGAG 1 cut(s) 19
BseXI GCAGC 2 cut(s) 376, 403
BseYI CCCAGC 1 cut(s) 240
Bsh1236I CGCG 1 cut(s) 35
BshFI GGCC 1 cut(s) 309
BshNI GGYRCC 2 cut(s) 20, 422
BsiHKAI GWGCWC 2 cut(s) 52, 132
BsiHKCI CYCGRG 2 cut(s) 138, 254
BsiWI CGTACG 1 cut(s) 153
BslFI GGGAC 1 cut(s) 189
BslI CCNNNNNNNGG 3 cut(s) 178, 482, 541
BsmAI GTCTC 1 cut(s) 141
BsmBI CGTCTC 1 cut(s) 141
BsmFI GGGAC 1 cut(s) 189
BsnI GGCC 1 cut(s) 309
BsoBI CYCGRG 2 cut(s) 138, 254
Bsp1286I GDGCHC 3 cut(s) 52, 80, 132
Bsp143I GATC 4 cut(s) 111, 363, 594, 616
Bsp19I CCATGG 1 cut(s) 65
Bsp68I TCGCGA 1 cut(s) 35
BspACI CCGC 4 cut(s) 222, 419, 439, 492
BspANI GGCC 1 cut(s) 309
BspCNI CTCAG 2 cut(s) 29, 253
BspFNI CGCG 1 cut(s) 35
BspHI TCATGA 1 cut(s) 624
BspLI GGNNCC 4 cut(s) 8, 22, 205, 424
BspMAI CTGCAG 2 cut(s) 322, 634
BspMI ACCTGC 1 cut(s) 311
BspPI GGATC 2 cut(s) 106, 624
BspQI GCTCTTC 1 cut(s) 40
BspT107I GGYRCC 2 cut(s) 20, 422
BssECI CCNNGG 2 cut(s) 65, 426
BssMI GATC 4 cut(s) 111, 363, 594, 616
BssNI GRCGYC 2 cut(s) 21, 89
BssSI CACGAG 1 cut(s) 51
BssT1I CCWWGG 2 cut(s) 65, 426
Bst2BI CACGAG 1 cut(s) 51
Bst2UI CCWGG 1 cut(s) 303
Bst4CI ACNGT 2 cut(s) 107, 203
Bst6I CTCTTC 1 cut(s) 40
BstACI GRCGYC 2 cut(s) 21, 89
BstAPI GCANNNNNTGC 2 cut(s) 192, 326
BstC8I GCNNGC 2 cut(s) 146, 150
BstDEI CTNAG 3 cut(s) 16, 261, 412
BstDSI CCRYGG 1 cut(s) 65
BstEII GGTNACC 1 cut(s) 357
BstENI CCTNNNNNAGG 1 cut(s) 176
BstF5I GGATG 1 cut(s) 269
BstFNI CGCG 1 cut(s) 35
BstH2I RGCGCY 1 cut(s) 24
BstHHI GCGC 2 cut(s) 23, 186
BstKTI GATC 4 cut(s) 114, 366, 597, 619
BstMAI GTCTC 1 cut(s) 141
BstMBI GATC 4 cut(s) 111, 363, 594, 616
BstMWI GCNNNNNNNGC 7 cut(s) 192, 326, 398, 422, 577, 638, 717
BstNI CCWGG 1 cut(s) 303
BstPI GGTNACC 1 cut(s) 357
BstSCI CCNGG 1 cut(s) 301
BstSFI CTRYAG 3 cut(s) 103, 318, 630
BstUI CGCG 1 cut(s) 35
BstV1I GCAGC 2 cut(s) 376, 403
BstV2I GAAGAC 1 cut(s) 84
BsuRI GGCC 1 cut(s) 309
BtgI CCRYGG 1 cut(s) 65
BtsCI GGATG 1 cut(s) 269
BtsI GCAGTG 1 cut(s) 315
BtsIMutI CAGTG 2 cut(s) 315, 696
BtuMI TCGCGA 1 cut(s) 35
BveI ACCTGC 1 cut(s) 311
Cac8I GCNNGC 2 cut(s) 146, 150
CciI TCATGA 1 cut(s) 624
CfoI GCGC 2 cut(s) 23, 186
Cfr13I GGNCC 1 cut(s) 203
Csp6I GTAC 4 cut(s) 154, 289, 349, 487
CviAII CATG 4 cut(s) 66, 227, 311, 625
CviQI GTAC 4 cut(s) 154, 289, 349, 487
DdeI CTNAG 3 cut(s) 16, 261, 412
DinI GGCGCC 1 cut(s) 22
DpnI GATC 4 cut(s) 113, 365, 596, 618
DpnII GATC 4 cut(s) 111, 363, 594, 616
DrdI GACNNNNNNGTC 1 cut(s) 88
DseDI GACNNNNNNGTC 1 cut(s) 88
EaeI YGGCCR 1 cut(s) 307
Eam1104I CTCTTC 1 cut(s) 40
EarI CTCTTC 1 cut(s) 40
Eco130I CCWWGG 2 cut(s) 65, 426
Eco24I GRGCYC 1 cut(s) 80
Eco47I GGWCC 1 cut(s) 203
Eco57I CTGAAG 1 cut(s) 462
Eco88I CYCGRG 2 cut(s) 138, 254
Eco91I GGTNACC 1 cut(s) 357
EcoNI CCTNNNNNAGG 1 cut(s) 176
EcoO65I GGTNACC 1 cut(s) 357
EcoRII CCWGG 1 cut(s) 301
EcoT14I CCWWGG 2 cut(s) 65, 426
EcoT22I ATGCAT 1 cut(s) 559
EcoT38I GRGCYC 1 cut(s) 80
EgeI GGCGCC 1 cut(s) 22
EheI GGCGCC 1 cut(s) 22
ErhI CCWWGG 2 cut(s) 65, 426
Esp3I CGTCTC 1 cut(s) 141
FaeI CATG 4 cut(s) 69, 230, 314, 628
FaqI GGGAC 1 cut(s) 189
FatI CATG 4 cut(s) 65, 226, 310, 624
FauI CCCGC 1 cut(s) 412
Fnu4HI GCNGC 2 cut(s) 390, 417
FokI GGATG 1 cut(s) 256
FriOI GRGCYC 1 cut(s) 80
Fsp4HI GCNGC 2 cut(s) 390, 417
FspBI CTAG 1 cut(s) 745
GlaI GCGC 2 cut(s) 22, 185
GluI GCNGC 2 cut(s) 390, 417
GsaI CCCAGC 1 cut(s) 244
HaeII RGCGCY 1 cut(s) 24
HaeIII GGCC 1 cut(s) 309
HhaI GCGC 2 cut(s) 23, 186
Hin1I GRCGYC 2 cut(s) 21, 89
Hin1II CATG 4 cut(s) 69, 230, 314, 628
Hin6I GCGC 2 cut(s) 21, 184
HinP1I GCGC 2 cut(s) 21, 184
HinfI GANTC 4 cut(s) 61, 257, 466, 545
HphI GGTGA 3 cut(s) 133, 351, 443
Hpy188I TCNGA 2 cut(s) 111, 262
Hpy188III TCNNGA 5 cut(s) 34, 138, 254, 598, 625
Hpy99I CGWCG 7 cut(s) 35, 85, 88, 91, 218, 236, 239
HpyAV CCTTC 3 cut(s) 182, 486, 710
HpyCH4III ACNGT 2 cut(s) 107, 203
HpyCH4IV ACGT 1 cut(s) 89
HpyCH4V TGCA 7 cut(s) 195, 320, 329, 346, 392, 557, 632
HpyF10VI GCNNNNNNNGC 7 cut(s) 192, 326, 398, 422, 577, 638, 717
HpyF3I CTNAG 3 cut(s) 16, 261, 412
HpySE526I ACGT 1 cut(s) 89
Hsp92I GRCGYC 2 cut(s) 21, 89
Hsp92II CATG 4 cut(s) 69, 230, 314, 628
HspAI GCGC 2 cut(s) 21, 184
KasI GGCGCC 1 cut(s) 20
Kzo9I GATC 4 cut(s) 111, 363, 594, 616
LguI GCTCTTC 1 cut(s) 40
LmnI GCTCC 2 cut(s) 6, 181
LpnPI CCDG 9 cut(s) 3, 52, 254, 288, 306, 315, 465, 561, 677
Lsp1109I GCAGC 2 cut(s) 376, 403
LweI GCATC 3 cut(s) 204, 401, 691
MaeI CTAG 1 cut(s) 745
MaeII ACGT 1 cut(s) 89
MaeIII GTNAC 4 cut(s) 313, 357, 441, 694
MalI GATC 4 cut(s) 113, 365, 596, 618
MboI GATC 4 cut(s) 111, 363, 594, 616
MboII GAAGA 4 cut(s) 57, 84, 154, 613
MhlI GDGCHC 3 cut(s) 52, 80, 132
MlsI TGGCCA 1 cut(s) 309
MluCI AATT 6 cut(s) 295, 330, 372, 459, 513, 657
MluNI TGGCCA 1 cut(s) 309
Mly113I GGCGCC 1 cut(s) 21
MlyI GAGTC 2 cut(s) 70, 266
MnlI CCTC 4 cut(s) 134, 302, 429, 631
Mox20I TGGCCA 1 cut(s) 309
Mph1103I ATGCAT 1 cut(s) 559
MscI TGGCCA 1 cut(s) 309
MslI CAYNNNNRTG 2 cut(s) 284, 461
Msp20I TGGCCA 1 cut(s) 309
MspA1I CMGCKG 1 cut(s) 492
MspR9I CCNGG 1 cut(s) 303
MvaI CCWGG 1 cut(s) 303
MvnI CGCG 1 cut(s) 35
MwoI GCNNNNNNNGC 7 cut(s) 192, 326, 398, 422, 577, 638, 717
NarI GGCGCC 1 cut(s) 21
NcoI CCATGG 1 cut(s) 65
NdeII GATC 4 cut(s) 111, 363, 594, 616
NlaIII CATG 4 cut(s) 69, 230, 314, 628
NlaIV GGNNCC 4 cut(s) 8, 22, 205, 424
NmuCI GTSAC 4 cut(s) 313, 357, 441, 694
NruI TCGCGA 1 cut(s) 35
NsiI ATGCAT 1 cut(s) 559
PaeR7I CTCGAG 2 cut(s) 138, 254
PagI TCATGA 1 cut(s) 624
PciSI GCTCTTC 1 cut(s) 40
PcsI WCGNNNNNNNCGW 2 cut(s) 80, 145
PfeI GAWTC 2 cut(s) 466, 545
Pfl23II CGTACG 1 cut(s) 153
PflFI GACNNNGTC 1 cut(s) 88
PkrI GCNGC 2 cut(s) 391, 418
PleI GAGTC 2 cut(s) 69, 265
PluTI GGCGCC 1 cut(s) 24
PpsI GAGTC 2 cut(s) 69, 265
Psp6I CCWGG 1 cut(s) 301
PspEI GGTNACC 1 cut(s) 357
PspFI CCCAGC 1 cut(s) 240
PspGI CCWGG 1 cut(s) 301
PspLI CGTACG 1 cut(s) 153
PspN4I GGNNCC 4 cut(s) 8, 22, 205, 424
PspPI GGNCC 1 cut(s) 203
PstI CTGCAG 2 cut(s) 322, 634
PsyI GACNNNGTC 1 cut(s) 88
RruI TCGCGA 1 cut(s) 35
RsaI GTAC 4 cut(s) 155, 290, 350, 488
RsaNI GTAC 4 cut(s) 154, 289, 349, 487
RseI CAYNNNNRTG 2 cut(s) 284, 461
SapI GCTCTTC 1 cut(s) 40
SatI GCNGC 2 cut(s) 390, 417
Sau3AI GATC 4 cut(s) 111, 363, 594, 616
Sau96I GGNCC 1 cut(s) 203
SchI GAGTC 2 cut(s) 70, 266
ScrFI CCNGG 1 cut(s) 303
SduI GDGCHC 3 cut(s) 52, 80, 132
SetI ASST 8 cut(s) 30, 92, 246, 294, 325, 391, 418, 484
SfaNI GCATC 3 cut(s) 204, 401, 691
SfcI CTRYAG 3 cut(s) 103, 318, 630
SfoI GGCGCC 1 cut(s) 22
Sfr274I CTCGAG 2 cut(s) 138, 254
SinI GGWCC 1 cut(s) 203
SlaI CTCGAG 2 cut(s) 138, 254
SmiMI CAYNNNNRTG 2 cut(s) 284, 461
SmlI CTYRAG 3 cut(s) 138, 176, 254
SmoI CTYRAG 3 cut(s) 138, 176, 254
Sse9I AATT 6 cut(s) 295, 330, 372, 459, 513, 657
SsiI CCGC 4 cut(s) 222, 419, 439, 492
SspDI GGCGCC 1 cut(s) 20
SspMI CTAG 1 cut(s) 745
StyD4I CCNGG 1 cut(s) 301
StyI CCWWGG 2 cut(s) 65, 426
TaaI ACNGT 2 cut(s) 107, 203
TaiI ACGT 1 cut(s) 92
TaqI TCGA 4 cut(s) 59, 139, 255, 597
TaqII GACCGA 1 cut(s) 345
TasI AATT 6 cut(s) 295, 330, 372, 459, 513, 657
TatI WGTACW 1 cut(s) 348
TfiI GAWTC 2 cut(s) 466, 545
TscAI CASTG 2 cut(s) 322, 703
TseFI GTSAC 4 cut(s) 313, 357, 441, 694
TseI GCWGC 2 cut(s) 389, 416
Tsp45I GTSAC 4 cut(s) 313, 357, 441, 694
TspDTI ATGAA 4 cut(s) 458, 613, 678, 690
TspGWI ACGGA 1 cut(s) 524
TspRI CASTG 2 cut(s) 322, 703
Tth111I GACNNNGTC 1 cut(s) 88
VpaK11BI GGWCC 1 cut(s) 203
XagI CCTNNNNNAGG 1 cut(s) 176
XhoI CTCGAG 2 cut(s) 138, 254
XspI CTAG 1 cut(s) 745
ZraI GACGTC 1 cut(s) 90
Zsp2I ATGCAT 1 cut(s) 559
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.