Rh4BG444100

ATP-dependent Clp protease proteolytic subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0014077
Physical Location & Seq
Forward (+)
58391072 .. 58393317
2246 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG444100.1

Sequence Viewer

Length: 759 bp
ATGATTATGATGAGGAGCCTAATCTCAGGCGCCAAGCTCGTCGCGACCAGCAGAAGAGCGCGAGTTTCGAGTCCATGGGGAACGAGCACCACGACGACGACGTCATCTCGGTGCTACAGTCTGATCCCAATGGTGATAGAGCACTCGTCTCGAGGTGAGCGAGCGTACGACATCTTCTCTCGCCTTCTCAAGGAGCGCATAGTCTGCATCAACGGTCCCATCAACGACGACACCGCCCATGTCGTCGTCGCCCAGCTTCTCTTTCTCGAGTCTGAGAATCCATCCAAGCCCATCAATATGTACCTCAATTCTCCTGGTGGCCATGTCACTGCAGGTCTTGCAATTTATGATACTATGCAGTACATTCGGTCACCGATCAATACAATTTGTTTGGGTCAAGCTGCATCAATGGCTTCACTTCTCTTAGCTGCGGGTGCCAAGGGTGAGAGGCGGTCACTTCCCAATGCCACAATTATGATTCATCAGCCTTCAGGTGGGTACAGCGGACAAGCAAAAGATATGACAATTCACACAAAGCAGATTGTTCGTGTGTGGGATTCACTCAATGCATTGTATTCAAAGCATACAGGGCAGTCAAAAGAGATGATCGAGAAGAATATGGATAGGGATCACTTCATGACCGCAGTAGAGGCAAAGGAGTTTGGAATAATTGATGAAGTCATTGATGAAAGACCAATGGCTCTGGTCACTGATGCTGTTGGCAAAGAAGGCAAAGAAAAGGACAAGAGTTCAAACTAG

Protein Analysis

252

Amino Acids

27.68

Weight (kDa)

8.71

Isoelectric Point (pI)

36.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLP_protease PF00574 51 - 230 6.5e-82 Clp protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014077)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23140
fragaria_vesca FvH4_4g36920
malus_domestica MD13G1005300.v1.1 MD16G1002600.v1.1
prunus_persica Prupe.1G347500_v2.0.a1
pyrus_communis pycom13g00450 pycom16g00330
rosa_chinensis RchiOBHm_Chr4g0446681
rosa_laevigata RLG00000005640
rosa_multiflora Rmu_sc0001753.1_g000041
rosa_roxburghii Rroxscaffold_5G00387040
rosa_rugosa Rorug04G0373600
rosa_samantha Rh4AG435100 Rh4BG444100 Rh4CG461400 Rh4DG442900
rosa_wichuraiana Rw4G037200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 100
AatII GACGTC 1 cut(s) 104
Acc36I ACCTGC 1 cut(s) 323
AccB1I GGYRCC 2 cut(s) 29, 434
AccII CGCG 2 cut(s) 44, 61
AciI CCGC 5 cut(s) 234, 431, 451, 504, 642
AclWI GGATC 2 cut(s) 118, 636
AcoI YGGCCR 1 cut(s) 319
AcuI CTGAAG 1 cut(s) 474
AcyI GRCGYC 2 cut(s) 30, 101
AfaI GTAC 4 cut(s) 167, 302, 362, 500
AfiI CCNNNNNNNGG 2 cut(s) 190, 494
AgsI TTSAA 2 cut(s) 579, 753
AjnI CCWGG 1 cut(s) 313
AluBI AGCT 4 cut(s) 37, 256, 401, 428
AluI AGCT 4 cut(s) 37, 256, 401, 428
Alw21I GWGCWC 2 cut(s) 89, 144
Alw26I GTCTC 1 cut(s) 153
AlwI GGATC 2 cut(s) 118, 636
Ama87I CYCGRG 2 cut(s) 150, 266
AoxI GGCC 1 cut(s) 319
ApeKI GCWGC 2 cut(s) 401, 428
AspLEI GCGC 3 cut(s) 32, 61, 198
AspS9I GGNCC 1 cut(s) 215
AsuHPI GGTGA 4 cut(s) 145, 167, 363, 455
AvaI CYCGRG 2 cut(s) 150, 266
AvaII GGWCC 1 cut(s) 215
BalI TGGCCA 1 cut(s) 321
BanI GGYRCC 2 cut(s) 29, 434
BarI GAAGNNNNNNTAC 2 cut(s) 158, 190
Bbv12I GWGCWC 2 cut(s) 89, 144
BbvI GCAGC 2 cut(s) 388, 415
BccI CCATC 3 cut(s) 227, 289, 299
BcgI CGANNNNNNTGC 6 cut(s) 131, 165, 347, 381, 527, 561
BciT130I CCWGG 1 cut(s) 315
BcoDI GTCTC 1 cut(s) 153
BfaI CTAG 1 cut(s) 757
BfmI CTRYAG 2 cut(s) 115, 330
BfoI RGCGCY 1 cut(s) 33
BfuAI ACCTGC 1 cut(s) 323
BisI GCNGC 2 cut(s) 402, 429
BlsI GCNGC 2 cut(s) 403, 430
Bme1390I CCNGG 1 cut(s) 315
Bme18I GGWCC 1 cut(s) 215
BmeT110I CYCGRG 2 cut(s) 150, 266
BmgT120I GGNCC 1 cut(s) 215
BmiI GGNNCC 4 cut(s) 17, 31, 217, 436
BmrFI CCNGG 1 cut(s) 315
BmsI GCATC 3 cut(s) 216, 413, 703
BpuEI CTTGAG 1 cut(s) 173
BsaBI GATNNNNATC 1 cut(s) 627
BsaHI GRCGYC 2 cut(s) 30, 101
BsaJI CCNNGG 2 cut(s) 74, 438
Bsc4I CCNNNNNNNGG 2 cut(s) 190, 494
Bse8I GATNNNNATC 1 cut(s) 627
BseBI CCWGG 1 cut(s) 315
BseDI CCNNGG 2 cut(s) 74, 438
BseGI GGATG 1 cut(s) 281
BseJI GATNNNNATC 1 cut(s) 627
BseLI CCNNNNNNNGG 2 cut(s) 190, 494
BseMII CTCAG 2 cut(s) 39, 264
BseRI GAGGAG 1 cut(s) 28
BseXI GCAGC 2 cut(s) 388, 415
BseYI CCCAGC 1 cut(s) 252
Bsh1236I CGCG 2 cut(s) 44, 61
BshFI GGCC 1 cut(s) 321
BshNI GGYRCC 2 cut(s) 29, 434
BsiHKAI GWGCWC 2 cut(s) 89, 144
BsiHKCI CYCGRG 2 cut(s) 150, 266
BsiWI CGTACG 1 cut(s) 165
BslFI GGGAC 1 cut(s) 201
BslI CCNNNNNNNGG 2 cut(s) 190, 494
BsmAI GTCTC 1 cut(s) 153
BsmBI CGTCTC 1 cut(s) 153
BsmFI GGGAC 1 cut(s) 201
BsnI GGCC 1 cut(s) 321
BsoBI CYCGRG 2 cut(s) 150, 266
Bsp1286I GDGCHC 2 cut(s) 89, 144
Bsp143I GATC 4 cut(s) 123, 375, 606, 628
Bsp19I CCATGG 1 cut(s) 74
Bsp68I TCGCGA 1 cut(s) 44
BspACI CCGC 5 cut(s) 234, 431, 451, 504, 642
BspANI GGCC 1 cut(s) 321
BspCNI CTCAG 2 cut(s) 38, 265
BspFNI CGCG 2 cut(s) 44, 61
BspHI TCATGA 1 cut(s) 636
BspLI GGNNCC 4 cut(s) 17, 31, 217, 436
BspMAI CTGCAG 1 cut(s) 334
BspMI ACCTGC 1 cut(s) 323
BspPI GGATC 2 cut(s) 118, 636
BspQI GCTCTTC 1 cut(s) 49
BspT107I GGYRCC 2 cut(s) 29, 434
BssECI CCNNGG 2 cut(s) 74, 438
BssMI GATC 4 cut(s) 123, 375, 606, 628
BssNI GRCGYC 2 cut(s) 30, 101
BssT1I CCWWGG 2 cut(s) 74, 438
Bst2UI CCWGG 1 cut(s) 315
Bst4CI ACNGT 2 cut(s) 119, 215
Bst6I CTCTTC 1 cut(s) 49
BstACI GRCGYC 2 cut(s) 30, 101
BstAPI GCANNNNNTGC 2 cut(s) 204, 338
BstC8I GCNNGC 1 cut(s) 162
BstDEI CTNAG 3 cut(s) 25, 273, 424
BstDSI CCRYGG 1 cut(s) 74
BstEII GGTNACC 1 cut(s) 369
BstENI CCTNNNNNAGG 1 cut(s) 188
BstF5I GGATG 1 cut(s) 281
BstFNI CGCG 2 cut(s) 44, 61
BstH2I RGCGCY 1 cut(s) 33
BstHHI GCGC 3 cut(s) 32, 61, 198
BstKTI GATC 4 cut(s) 126, 378, 609, 631
BstMAI GTCTC 1 cut(s) 153
BstMBI GATC 4 cut(s) 123, 375, 606, 628
BstMWI GCNNNNNNNGC 7 cut(s) 204, 338, 410, 434, 589, 650, 729
BstNI CCWGG 1 cut(s) 315
BstPI GGTNACC 1 cut(s) 369
BstSCI CCNGG 1 cut(s) 313
BstSFI CTRYAG 2 cut(s) 115, 330
BstUI CGCG 2 cut(s) 44, 61
BstV1I GCAGC 2 cut(s) 388, 415
BsuRI GGCC 1 cut(s) 321
BtgI CCRYGG 1 cut(s) 74
BtsCI GGATG 1 cut(s) 281
BtsI GCAGTG 1 cut(s) 327
BtsIMutI CAGTG 2 cut(s) 327, 708
BtuMI TCGCGA 1 cut(s) 44
BveI ACCTGC 1 cut(s) 323
Cac8I GCNNGC 1 cut(s) 162
CciI TCATGA 1 cut(s) 636
CfoI GCGC 3 cut(s) 32, 61, 198
Cfr13I GGNCC 1 cut(s) 215
Csp6I GTAC 4 cut(s) 166, 301, 361, 499
CviAII CATG 4 cut(s) 75, 239, 323, 637
CviQI GTAC 4 cut(s) 166, 301, 361, 499
DdeI CTNAG 3 cut(s) 25, 273, 424
DinI GGCGCC 1 cut(s) 31
DpnI GATC 4 cut(s) 125, 377, 608, 630
DpnII GATC 4 cut(s) 123, 375, 606, 628
DrdI GACNNNNNNGTC 1 cut(s) 100
DseDI GACNNNNNNGTC 1 cut(s) 100
EaeI YGGCCR 1 cut(s) 319
Eam1104I CTCTTC 1 cut(s) 49
EarI CTCTTC 1 cut(s) 49
Eco130I CCWWGG 2 cut(s) 74, 438
Eco47I GGWCC 1 cut(s) 215
Eco57I CTGAAG 1 cut(s) 474
Eco88I CYCGRG 2 cut(s) 150, 266
Eco91I GGTNACC 1 cut(s) 369
EcoNI CCTNNNNNAGG 1 cut(s) 188
EcoO65I GGTNACC 1 cut(s) 369
EcoRII CCWGG 1 cut(s) 313
EcoT14I CCWWGG 2 cut(s) 74, 438
EcoT22I ATGCAT 1 cut(s) 571
EgeI GGCGCC 1 cut(s) 31
EheI GGCGCC 1 cut(s) 31
ErhI CCWWGG 2 cut(s) 74, 438
Esp3I CGTCTC 1 cut(s) 153
FaeI CATG 4 cut(s) 78, 242, 326, 640
FaqI GGGAC 1 cut(s) 201
FatI CATG 4 cut(s) 74, 238, 322, 636
FauI CCCGC 1 cut(s) 424
Fnu4HI GCNGC 2 cut(s) 402, 429
FokI GGATG 1 cut(s) 268
Fsp4HI GCNGC 2 cut(s) 402, 429
FspBI CTAG 1 cut(s) 757
GlaI GCGC 3 cut(s) 31, 60, 197
GluI GCNGC 2 cut(s) 402, 429
GsaI CCCAGC 1 cut(s) 256
HaeII RGCGCY 1 cut(s) 33
HaeIII GGCC 1 cut(s) 321
HhaI GCGC 3 cut(s) 32, 61, 198
Hin1I GRCGYC 2 cut(s) 30, 101
Hin1II CATG 4 cut(s) 78, 242, 326, 640
Hin6I GCGC 3 cut(s) 30, 59, 196
HinP1I GCGC 3 cut(s) 30, 59, 196
HinfI GANTC 5 cut(s) 70, 269, 277, 478, 557
HphI GGTGA 4 cut(s) 145, 167, 363, 455
Hpy188I TCNGA 2 cut(s) 123, 274
Hpy188III TCNNGA 5 cut(s) 43, 150, 266, 610, 637
Hpy99I CGWCG 7 cut(s) 44, 97, 100, 103, 230, 248, 251
HpyAV CCTTC 3 cut(s) 194, 498, 722
HpyCH4III ACNGT 2 cut(s) 119, 215
HpyCH4IV ACGT 1 cut(s) 101
HpyCH4V TGCA 6 cut(s) 207, 332, 341, 358, 404, 569
HpyF10VI GCNNNNNNNGC 7 cut(s) 204, 338, 410, 434, 589, 650, 729
HpyF3I CTNAG 3 cut(s) 25, 273, 424
HpySE526I ACGT 1 cut(s) 101
Hsp92I GRCGYC 2 cut(s) 30, 101
Hsp92II CATG 4 cut(s) 78, 242, 326, 640
HspAI GCGC 3 cut(s) 30, 59, 196
KasI GGCGCC 1 cut(s) 29
Kzo9I GATC 4 cut(s) 123, 375, 606, 628
LguI GCTCTTC 1 cut(s) 49
LmnI GCTCC 2 cut(s) 15, 193
LpnPI CCDG 9 cut(s) 12, 61, 266, 300, 318, 327, 477, 573, 689
Lsp1109I GCAGC 2 cut(s) 388, 415
LweI GCATC 3 cut(s) 216, 413, 703
MaeI CTAG 1 cut(s) 757
MaeII ACGT 1 cut(s) 101
MaeIII GTNAC 4 cut(s) 325, 369, 453, 706
MalI GATC 4 cut(s) 125, 377, 608, 630
MboI GATC 4 cut(s) 123, 375, 606, 628
MboII GAAGA 3 cut(s) 66, 166, 625
MhlI GDGCHC 2 cut(s) 89, 144
MlsI TGGCCA 1 cut(s) 321
MluCI AATT 6 cut(s) 307, 342, 384, 471, 525, 669
MluNI TGGCCA 1 cut(s) 321
Mly113I GGCGCC 1 cut(s) 30
MlyI GAGTC 2 cut(s) 79, 278
MnlI CCTC 5 cut(s) 6, 146, 314, 441, 643
Mox20I TGGCCA 1 cut(s) 321
Mph1103I ATGCAT 1 cut(s) 571
MscI TGGCCA 1 cut(s) 321
MslI CAYNNNNRTG 3 cut(s) 109, 296, 473
Msp20I TGGCCA 1 cut(s) 321
MspA1I CMGCKG 1 cut(s) 504
MspR9I CCNGG 1 cut(s) 315
MvaI CCWGG 1 cut(s) 315
MvnI CGCG 2 cut(s) 44, 61
MwoI GCNNNNNNNGC 7 cut(s) 204, 338, 410, 434, 589, 650, 729
NarI GGCGCC 1 cut(s) 30
NcoI CCATGG 1 cut(s) 74
NdeII GATC 4 cut(s) 123, 375, 606, 628
NlaIII CATG 4 cut(s) 78, 242, 326, 640
NlaIV GGNNCC 4 cut(s) 17, 31, 217, 436
NmuCI GTSAC 4 cut(s) 325, 369, 453, 706
NruI TCGCGA 1 cut(s) 44
NsiI ATGCAT 1 cut(s) 571
PaeR7I CTCGAG 2 cut(s) 150, 266
PagI TCATGA 1 cut(s) 636
PciSI GCTCTTC 1 cut(s) 49
PcsI WCGNNNNNNNCGW 3 cut(s) 89, 98, 157
PfeI GAWTC 3 cut(s) 277, 478, 557
Pfl23II CGTACG 1 cut(s) 165
PflFI GACNNNGTC 1 cut(s) 100
PkrI GCNGC 2 cut(s) 403, 430
PleI GAGTC 2 cut(s) 78, 277
PluTI GGCGCC 1 cut(s) 33
PpsI GAGTC 2 cut(s) 78, 277
Psp6I CCWGG 1 cut(s) 313
PspEI GGTNACC 1 cut(s) 369
PspFI CCCAGC 1 cut(s) 252
PspGI CCWGG 1 cut(s) 313
PspLI CGTACG 1 cut(s) 165
PspN4I GGNNCC 4 cut(s) 17, 31, 217, 436
PspPI GGNCC 1 cut(s) 215
PstI CTGCAG 1 cut(s) 334
PsyI GACNNNGTC 1 cut(s) 100
RruI TCGCGA 1 cut(s) 44
RsaI GTAC 4 cut(s) 167, 302, 362, 500
RsaNI GTAC 4 cut(s) 166, 301, 361, 499
RseI CAYNNNNRTG 3 cut(s) 109, 296, 473
SapI GCTCTTC 1 cut(s) 49
SatI GCNGC 2 cut(s) 402, 429
Sau3AI GATC 4 cut(s) 123, 375, 606, 628
Sau96I GGNCC 1 cut(s) 215
SchI GAGTC 2 cut(s) 79, 278
ScrFI CCNGG 1 cut(s) 315
SduI GDGCHC 2 cut(s) 89, 144
SetI ASST 9 cut(s) 39, 104, 157, 258, 306, 337, 403, 430, 496
SfaNI GCATC 3 cut(s) 216, 413, 703
SfcI CTRYAG 2 cut(s) 115, 330
SfoI GGCGCC 1 cut(s) 31
Sfr274I CTCGAG 2 cut(s) 150, 266
SinI GGWCC 1 cut(s) 215
SlaI CTCGAG 2 cut(s) 150, 266
SmiMI CAYNNNNRTG 3 cut(s) 109, 296, 473
SmlI CTYRAG 3 cut(s) 150, 188, 266
SmoI CTYRAG 3 cut(s) 150, 188, 266
Sse9I AATT 6 cut(s) 307, 342, 384, 471, 525, 669
SsiI CCGC 5 cut(s) 234, 431, 451, 504, 642
SspDI GGCGCC 1 cut(s) 29
SspMI CTAG 1 cut(s) 757
StyD4I CCNGG 1 cut(s) 313
StyI CCWWGG 2 cut(s) 74, 438
TaaI ACNGT 2 cut(s) 119, 215
TaiI ACGT 1 cut(s) 104
TaqI TCGA 4 cut(s) 68, 151, 267, 609
TaqII GACCGA 1 cut(s) 357
TasI AATT 6 cut(s) 307, 342, 384, 471, 525, 669
TatI WGTACW 1 cut(s) 360
TfiI GAWTC 3 cut(s) 277, 478, 557
TscAI CASTG 2 cut(s) 334, 715
TseFI GTSAC 4 cut(s) 325, 369, 453, 706
TseI GCWGC 2 cut(s) 401, 428
Tsp45I GTSAC 4 cut(s) 325, 369, 453, 706
TspDTI ATGAA 4 cut(s) 470, 625, 690, 702
TspRI CASTG 2 cut(s) 334, 715
Tth111I GACNNNGTC 1 cut(s) 100
VpaK11BI GGWCC 1 cut(s) 215
XagI CCTNNNNNAGG 1 cut(s) 188
XhoI CTCGAG 2 cut(s) 150, 266
XspI CTAG 1 cut(s) 757
ZraI GACGTC 1 cut(s) 102
Zsp2I ATGCAT 1 cut(s) 571
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.