FvH4_5g00470

Heat shock chaperonin-binding motif.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0013195
Physical Location & Seq
Forward (+)
300275 .. 305263
4989 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g00470.t2

Sequence Viewer

Length: 1272 bp
ATGGAGGAGGCGCAGCTGAACCAGTTGAAGCAGTTCGTCGAGCAGTGCAAGTCCGATCCCTCCGTTCTCGCCGATCCTTCCCTCTCCTTCTTCCGCGATTACCTCGAAAGTCTCGGAGCTACTGTGCCTTCCTCTGCTAGCAACAAGGAAGATTCGAAACCGAGGAGCTATGTAGTGGAGGAGAGCGACGACGACATGTCGGAGGAGGCTGAGCCGGAGCCGGTTCATGTTCAGGAAGAAGAGGAAGAAGAGATAGTCGAATCTGATCTCGAGCTCGAAGGCGACATTGTGGAGCCTGACGATGAGCCTCCTCAGAAGATGGGAGACGCATCGGTCGAGGTCACTGAAGAGAACCGTGATGCCTCGCAGGCGGCCAAGGGTAAAGCTATGGAGGCCATTTCAGAAGGTAACTTGGACGAAGCCATTGAGCATCTTACTGAGGCAATTTTGCTCAATCCTACTTCGGCTATTATGTACAGCACCAGAGCCAGTGTATACATAAAAATGAAAAAACCTAATGCCGCCATCCGTGATGCCAACGCTGCTCTTGAGATCAATCCTGACTCTGCTAAAGGCTACAAATCTCGTGGCATAGCTCATTCAATGCTTGGTAATTGGGAAGAGGCTGCGAAGGATCTCCACTTGGCATCAAAGCTAGACTTTGATGAGGAAATTAGTGCCGTACTTAAGAAGGTTGAACCAAATGTACACAGGATTCAGGAACACCGTCGTAAGTATGACAGGCTTCGAAAGGAAAGAGAGGACAGAAAGATTGAGCGTGACAGACAACGTCGCAAAGCTGAGGCCCAGGCTGCATATGAGAAGGCTAAGAAGAAAGAGCAGTCGTCTTCTAGTAGAAGACCTGGAGGCTTTCCTGGTGGAATGCCTGGAGGCTTCCCAGGAGGGATGCCAGGTGGTTTTCCAGGGGCAGGAGGTATGCCAGGTGGTTTTCCAGGGGCAGGAGGTATGCCAGGTGGTTTTCCAGGGGCAGGAGGGATGCCAGGTGGCTTTCCAGGCGCAGGAGGAATGCCTGGAGGCTTTCCAGGGGCAGGAGGTGCACCTGGAGGCTCTGCAGGAGGGGCTCCTGGAAACATTGACTTCAGCAAAATATTAAATGACCCAGAAATGATGGCTGCATTTAATGATCCAGAAGTTATGTCTGCTCTGCAAGATGTGATGAAGAACCCTGCTAATCTAGCAAAGCATCAAGAAAATCCCAAGGTGGCTCCTATTATTGCTAAGATGATGAGCAAATTTGGTGGACCAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0008150 GO:0010035 GO:0010038 GO:0042221 GO:0044424 GO:0044444 GO:0044464 GO:0046686 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPR_8

Protein Analysis

424

Amino Acids

45.22

Weight (kDa)

4.89

Isoelectric Point (pI)

64.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HipN PF18253 3 - 43 1.3e-19 Hsp70-interacting protein N N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013195)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G22670
fragaria_vesca FvH4_5g00470 FvH4_5g00470
malus_domestica MD04G1080700.v1.1
prunus_persica Prupe.5G077400_v2.0.a1
pyrus_communis pycom04g07300
rosa_chinensis RchiOBHm_Chr7g0202041
rosa_laevigata RLG00000003631
rosa_multiflora Rmu_sc0006592.1_g000004
rosa_roxburghii Rroxscaffold_3G00254910
rosa_rugosa Rorug07G0067500 Rorug07G0067600 Rorug07G0067700
rosa_samantha Rh7AG194800 Rh7BG196300 Rh7CG206100 Rh7DG201500
rosa_wichuraiana Rw7G017070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 332
AccI GTMKAC 1 cut(s) 495
AccII CGCG 1 cut(s) 96
AciI CCGC 3 cut(s) 94, 371, 522
AclWI GGATC 4 cut(s) 50, 68, 642, 1139
AcoI YGGCCR 1 cut(s) 372
AcsI RAATTY 1 cut(s) 1253
AcuI CTGAAG 2 cut(s) 366, 1084
AfaI GTAC 3 cut(s) 476, 684, 708
AfiI CCNNNNNNNGG 5 cut(s) 929, 959, 989, 1019, 1049
AflII CTTAAG 1 cut(s) 686
AflIII ACRYGT 1 cut(s) 195
AgsI TTSAA 3 cut(s) 28, 603, 698
AhdI GACNNNNNGTC 1 cut(s) 196
AluBI AGCT 8 cut(s) 16, 119, 168, 274, 386, 596, 655, 800
AluI AGCT 8 cut(s) 16, 119, 168, 274, 386, 596, 655, 800
Alw21I GWGCWC 2 cut(s) 276, 1060
Alw26I GTCTC 2 cut(s) 116, 318
Alw44I GTGCAC 1 cut(s) 1056
AlwI GGATC 4 cut(s) 50, 68, 642, 1139
Ama87I CYCGRG 1 cut(s) 269
AoxI GGCC 3 cut(s) 372, 393, 804
ApaLI GTGCAC 1 cut(s) 1056
ApeKI GCWGC 5 cut(s) 13, 542, 626, 812, 1133
ApoI RAATTY 1 cut(s) 1253
Asp700I GAANNNNTTC 1 cut(s) 32
AspLEI GCGC 2 cut(s) 13, 1019
AspS9I GGNCC 2 cut(s) 805, 1262
AsuII TTCGAA 2 cut(s) 155, 748
AsuNHI GCTAGC 1 cut(s) 137
AvaI CYCGRG 1 cut(s) 269
AvaII GGWCC 1 cut(s) 1262
BaeGI GKGCMC 1 cut(s) 1060
BanII GRGCYC 2 cut(s) 276, 1084
BauI CACGAG 1 cut(s) 585
BbsI GAAGAC 2 cut(s) 840, 865
Bbv12I GWGCWC 2 cut(s) 276, 1060
BbvCI CCTCAGC 1 cut(s) 801
BbvI GCAGC 5 cut(s) 25, 529, 613, 799, 1120
BccI CCATC 3 cut(s) 313, 533, 1123
BceAI ACGGC 1 cut(s) 665
BcoDI GTCTC 2 cut(s) 116, 318
BfaI CTAG 4 cut(s) 138, 656, 852, 1196
BfmI CTRYAG 1 cut(s) 1071
BfrI CTTAAG 1 cut(s) 686
BglI GCCNNNNNGGC 1 cut(s) 368
BisI GCNGC 7 cut(s) 14, 372, 522, 543, 627, 813, 1134
BlpI GCTNAGC 1 cut(s) 210
BlsI GCNGC 7 cut(s) 15, 373, 523, 544, 628, 814, 1135
Bme18I GGWCC 1 cut(s) 1262
BmeRI GACNNNNNGTC 1 cut(s) 196
BmeT110I CYCGRG 1 cut(s) 269
BmgT120I GGNCC 2 cut(s) 805, 1262
BmiI GGNNCC 4 cut(s) 219, 294, 1083, 1227
BmsI GCATC 8 cut(s) 338, 349, 439, 523, 656, 897, 987, 1213
BmtI GCTAGC 1 cut(s) 141
BpiI GAAGAC 2 cut(s) 840, 865
BpmI CTGGAG 4 cut(s) 885, 909, 1053, 1083
Bpu10I CCTNAGC 1 cut(s) 801
Bpu1102I GCTNAGC 1 cut(s) 210
Bpu14I TTCGAA 2 cut(s) 155, 748
BpuEI CTTGAG 1 cut(s) 569
BsaJI CCNNGG 9 cut(s) 161, 375, 807, 898, 923, 953, 983, 1043, 1218
BsaXI ACNNNNNCTCC 2 cut(s) 108, 138
Bsc4I CCNNNNNNNGG 5 cut(s) 929, 959, 989, 1019, 1049
Bse118I RCCGGY 1 cut(s) 220
Bse1I ACTGG 2 cut(s) 22, 489
BseDI CCNNGG 9 cut(s) 161, 375, 807, 898, 923, 953, 983, 1043, 1218
BseGI GGATG 3 cut(s) 525, 912, 1002
BseLI CCNNNNNNNGG 5 cut(s) 929, 959, 989, 1019, 1049
BseMII CTCAG 4 cut(s) 201, 326, 429, 792
BseNI ACTGG 2 cut(s) 22, 489
BseRI GAGGAG 5 cut(s) 20, 178, 194, 218, 300
BseSI GKGCMC 1 cut(s) 1060
BseXI GCAGC 5 cut(s) 25, 529, 613, 799, 1120
Bsh1236I CGCG 1 cut(s) 96
Bsh1285I CGRYCG 1 cut(s) 336
BshFI GGCC 3 cut(s) 374, 395, 806
BsiEI CGRYCG 1 cut(s) 336
BsiHKAI GWGCWC 2 cut(s) 276, 1060
BsiHKCI CYCGRG 1 cut(s) 269
BsiSI CCGG 2 cut(s) 215, 221
BslI CCNNNNNNNGG 5 cut(s) 929, 959, 989, 1019, 1049
BsmAI GTCTC 2 cut(s) 116, 318
BsmBI CGTCTC 1 cut(s) 318
BsmI GAATGC 2 cut(s) 888, 1032
BsnI GGCC 3 cut(s) 374, 395, 806
BsoBI CYCGRG 1 cut(s) 269
Bsp119I TTCGAA 2 cut(s) 155, 748
Bsp1286I GDGCHC 3 cut(s) 276, 1060, 1084
Bsp1407I TGTACA 2 cut(s) 474, 706
Bsp143I GATC 6 cut(s) 55, 73, 265, 552, 634, 1144
Bsp1720I GCTNAGC 1 cut(s) 210
BspACI CCGC 3 cut(s) 94, 371, 522
BspANI GGCC 3 cut(s) 374, 395, 806
BspCNI CTCAG 4 cut(s) 202, 325, 430, 793
BspFNI CGCG 1 cut(s) 96
BspLI GGNNCC 4 cut(s) 219, 294, 1083, 1227
BspMAI CTGCAG 1 cut(s) 1075
BspOI GCTAGC 1 cut(s) 141
BspPI GGATC 4 cut(s) 50, 68, 642, 1139
BspT104I TTCGAA 2 cut(s) 155, 748
BspTI CTTAAG 1 cut(s) 686
BsrFI RCCGGY 1 cut(s) 220
BsrGI TGTACA 2 cut(s) 474, 706
BsrI ACTGG 2 cut(s) 22, 489
BssAI RCCGGY 1 cut(s) 220
BssECI CCNNGG 9 cut(s) 161, 375, 807, 898, 923, 953, 983, 1043, 1218
BssMI GATC 6 cut(s) 55, 73, 265, 552, 634, 1144
BssNAI GTATAC 1 cut(s) 496
BssSI CACGAG 1 cut(s) 585
BssT1I CCWWGG 2 cut(s) 375, 1218
Bst1107I GTATAC 1 cut(s) 496
Bst2BI CACGAG 1 cut(s) 585
Bst4CI ACNGT 3 cut(s) 124, 356, 728
Bst6I CTCTTC 4 cut(s) 234, 243, 342, 615
BstAFI CTTAAG 1 cut(s) 686
BstAPI GCANNNNNTGC 1 cut(s) 1055
BstAUI TGTACA 2 cut(s) 474, 706
BstBI TTCGAA 2 cut(s) 155, 748
BstC8I GCNNGC 2 cut(s) 139, 369
BstDEI CTNAG 6 cut(s) 210, 312, 438, 801, 828, 1239
BstF5I GGATG 3 cut(s) 525, 912, 1002
BstFNI CGCG 1 cut(s) 96
BstHHI GCGC 2 cut(s) 13, 1019
BstKTI GATC 6 cut(s) 58, 76, 268, 555, 637, 1147
BstMAI GTCTC 2 cut(s) 116, 318
BstMBI GATC 6 cut(s) 55, 73, 265, 552, 634, 1144
BstMCI CGRYCG 1 cut(s) 336
BstMWI GCNNNNNNNGC 8 cut(s) 368, 392, 542, 812, 1014, 1055, 1079, 1196
BstNSI RCATGY 1 cut(s) 199
BstSFI CTRYAG 1 cut(s) 1071
BstSLI GKGCMC 1 cut(s) 1060
BstUI CGCG 1 cut(s) 96
BstV1I GCAGC 5 cut(s) 25, 529, 613, 799, 1120
BstV2I GAAGAC 2 cut(s) 840, 865
BstX2I RGATCY 1 cut(s) 634
BstYI RGATCY 1 cut(s) 634
BstZ17I GTATAC 1 cut(s) 496
BsuRI GGCC 3 cut(s) 374, 395, 806
BtsCI GGATG 3 cut(s) 525, 912, 1002
BtsI GCAGTG 1 cut(s) 50
BtsIMutI CAGTG 3 cut(s) 50, 342, 496
Cac8I GCNNGC 2 cut(s) 139, 369
CfoI GCGC 2 cut(s) 13, 1019
Cfr10I RCCGGY 1 cut(s) 220
Cfr13I GGNCC 2 cut(s) 805, 1262
CseI GACGC 1 cut(s) 335
Csp6I GTAC 3 cut(s) 475, 683, 707
CspCI CAANNNNNGTGG 3 cut(s) 568, 603, 1240
CviAII CATG 2 cut(s) 196, 227
CviQI GTAC 3 cut(s) 475, 683, 707
DdeI CTNAG 6 cut(s) 210, 312, 438, 801, 828, 1239
DpnI GATC 6 cut(s) 57, 75, 267, 554, 636, 1146
DpnII GATC 6 cut(s) 55, 73, 265, 552, 634, 1144
DrdI GACNNNNNNGTC 1 cut(s) 332
DriI GACNNNNNGTC 1 cut(s) 196
DseDI GACNNNNNNGTC 1 cut(s) 332
EaeI YGGCCR 1 cut(s) 372
Eam1104I CTCTTC 4 cut(s) 234, 243, 342, 615
Eam1105I GACNNNNNGTC 1 cut(s) 196
EarI CTCTTC 4 cut(s) 234, 243, 342, 615
Ecl136II GAGCTC 1 cut(s) 274
Eco130I CCWWGG 2 cut(s) 375, 1218
Eco24I GRGCYC 2 cut(s) 276, 1084
Eco47I GGWCC 1 cut(s) 1262
Eco53kI GAGCTC 1 cut(s) 274
Eco57I CTGAAG 2 cut(s) 366, 1084
Eco88I CYCGRG 1 cut(s) 269
EcoICRI GAGCTC 1 cut(s) 274
EcoT14I CCWWGG 2 cut(s) 375, 1218
EcoT38I GRGCYC 2 cut(s) 276, 1084
ErhI CCWWGG 2 cut(s) 375, 1218
Esp3I CGTCTC 1 cut(s) 318
FaeI CATG 2 cut(s) 199, 230
FalI AAGNNNNNCTT 2 cut(s) 644, 676
FatI CATG 2 cut(s) 195, 226
FauNDI CATATG 1 cut(s) 817
FblI GTMKAC 1 cut(s) 495
Fnu4HI GCNGC 7 cut(s) 14, 372, 522, 543, 627, 813, 1134
FokI GGATG 3 cut(s) 512, 919, 1009
FriOI GRGCYC 2 cut(s) 276, 1084
Fsp4HI GCNGC 7 cut(s) 14, 372, 522, 543, 627, 813, 1134
FspBI CTAG 4 cut(s) 138, 656, 852, 1196
GlaI GCGC 2 cut(s) 12, 1018
GluI GCNGC 7 cut(s) 14, 372, 522, 543, 627, 813, 1134
GsuI CTGGAG 4 cut(s) 885, 909, 1053, 1083
HaeIII GGCC 3 cut(s) 374, 395, 806
HapII CCGG 2 cut(s) 215, 221
HgaI GACGC 1 cut(s) 335
HhaI GCGC 2 cut(s) 13, 1019
Hin1II CATG 2 cut(s) 199, 230
Hin6I GCGC 2 cut(s) 11, 1017
HinP1I GCGC 2 cut(s) 11, 1017
HinfI GANTC 4 cut(s) 152, 260, 563, 715
HpaII CCGG 2 cut(s) 215, 221
Hpy166II GTNNAC 4 cut(s) 496, 709, 1058, 1262
Hpy188I TCNGA 6 cut(s) 55, 116, 202, 265, 315, 403
Hpy188III TCNNGA 7 cut(s) 233, 269, 548, 560, 719, 1148, 1208
Hpy8I GTNNAC 4 cut(s) 496, 709, 1058, 1262
Hpy99I CGWCG 5 cut(s) 41, 191, 194, 732, 795
HpyAV CCTTC 8 cut(s) 87, 97, 138, 272, 398, 625, 685, 817
HpyCH4III ACNGT 3 cut(s) 124, 356, 728
HpyCH4IV ACGT 1 cut(s) 790
HpyCH4V TGCA 6 cut(s) 48, 815, 1058, 1073, 1136, 1168
HpyF10VI GCNNNNNNNGC 8 cut(s) 368, 392, 542, 812, 1014, 1055, 1079, 1196
HpyF3I CTNAG 6 cut(s) 210, 312, 438, 801, 828, 1239
HpySE526I ACGT 1 cut(s) 790
Hsp92II CATG 2 cut(s) 199, 230
HspAI GCGC 2 cut(s) 11, 1017
Kzo9I GATC 6 cut(s) 55, 73, 265, 552, 634, 1144
LmnI GCTCC 6 cut(s) 116, 165, 217, 292, 1087, 1231
Lsp1109I GCAGC 5 cut(s) 25, 529, 613, 799, 1120
LweI GCATC 8 cut(s) 338, 349, 439, 523, 656, 897, 987, 1213
MaeI CTAG 4 cut(s) 138, 656, 852, 1196
MaeII ACGT 1 cut(s) 790
MaeIII GTNAC 3 cut(s) 340, 407, 779
MalI GATC 6 cut(s) 57, 75, 267, 554, 636, 1146
MboI GATC 6 cut(s) 55, 73, 265, 552, 634, 1144
MflI RGATCY 1 cut(s) 634
MhlI GDGCHC 3 cut(s) 276, 1060, 1084
MluCI AATT 4 cut(s) 444, 613, 672, 1253
MlyI GAGTC 1 cut(s) 557
MmeI TCCRAC 1 cut(s) 180
MroXI GAANNNNTTC 1 cut(s) 32
MseI TTAA 3 cut(s) 687, 1112, 1140
MslI CAYNNNNRTG 1 cut(s) 503
MspA1I CMGCKG 1 cut(s) 16
MspCI CTTAAG 1 cut(s) 686
MspI CCGG 2 cut(s) 215, 221
Mva1269I GAATGC 2 cut(s) 888, 1032
MvnI CGCG 1 cut(s) 96
MwoI GCNNNNNNNGC 8 cut(s) 368, 392, 542, 812, 1014, 1055, 1079, 1196
NdeI CATATG 1 cut(s) 817
NdeII GATC 6 cut(s) 55, 73, 265, 552, 634, 1144
NheI GCTAGC 1 cut(s) 137
NlaIII CATG 2 cut(s) 199, 230
NlaIV GGNNCC 4 cut(s) 219, 294, 1083, 1227
NmuCI GTSAC 2 cut(s) 340, 779
NspI RCATGY 1 cut(s) 199
NspV TTCGAA 2 cut(s) 155, 748
PaeR7I CTCGAG 1 cut(s) 269
PciI ACATGT 1 cut(s) 195
PcsI WCGNNNNNNNCGW 1 cut(s) 333
PctI GAATGC 2 cut(s) 888, 1032
PdmI GAANNNNTTC 1 cut(s) 32
PfeI GAWTC 3 cut(s) 152, 260, 715
PflFI GACNNNGTC 1 cut(s) 789
PfoI TCCNGGA 1 cut(s) 1084
PkrI GCNGC 7 cut(s) 15, 373, 523, 544, 628, 814, 1135
PleI GAGTC 1 cut(s) 557
PpsI GAGTC 1 cut(s) 557
PscI ACATGT 1 cut(s) 195
Psp124BI GAGCTC 1 cut(s) 276
PspN4I GGNNCC 4 cut(s) 219, 294, 1083, 1227
PspPI GGNCC 2 cut(s) 805, 1262
PsrI GAACNNNNNNTAC 2 cut(s) 690, 722
PstI CTGCAG 1 cut(s) 1075
PsuI RGATCY 1 cut(s) 634
PsyI GACNNNGTC 1 cut(s) 789
PvuII CAGCTG 1 cut(s) 16
RsaI GTAC 3 cut(s) 476, 684, 708
RsaNI GTAC 3 cut(s) 475, 683, 707
RseI CAYNNNNRTG 1 cut(s) 503
SacI GAGCTC 1 cut(s) 276
SaqAI TTAA 3 cut(s) 687, 1112, 1140
SatI GCNGC 7 cut(s) 14, 372, 522, 543, 627, 813, 1134
Sau3AI GATC 6 cut(s) 55, 73, 265, 552, 634, 1144
Sau96I GGNCC 2 cut(s) 805, 1262
SchI GAGTC 1 cut(s) 557
SduI GDGCHC 3 cut(s) 276, 1060, 1084
SfaNI GCATC 8 cut(s) 338, 349, 439, 523, 656, 897, 987, 1213
SfcI CTRYAG 1 cut(s) 1071
Sfr274I CTCGAG 1 cut(s) 269
SfuI TTCGAA 2 cut(s) 155, 748
SinI GGWCC 1 cut(s) 1262
SlaI CTCGAG 1 cut(s) 269
SmiMI CAYNNNNRTG 1 cut(s) 503
SmlI CTYRAG 3 cut(s) 269, 548, 686
SmoI CTYRAG 3 cut(s) 269, 548, 686
Sse9I AATT 4 cut(s) 444, 613, 672, 1253
SsiI CCGC 3 cut(s) 94, 371, 522
SspI AATATT 1 cut(s) 1110
SspMI CTAG 4 cut(s) 138, 656, 852, 1196
SstI GAGCTC 1 cut(s) 276
StyI CCWWGG 2 cut(s) 375, 1218
TaaI ACNGT 3 cut(s) 124, 356, 728
TaiI ACGT 1 cut(s) 793
TaqI TCGA 8 cut(s) 39, 105, 155, 258, 270, 276, 336, 748
TaqII GACCGA 1 cut(s) 322
TasI AATT 4 cut(s) 444, 613, 672, 1253
TatI WGTACW 2 cut(s) 474, 706
TauI GCSGC 2 cut(s) 374, 524
TfiI GAWTC 3 cut(s) 152, 260, 715
Tru1I TTAA 3 cut(s) 687, 1112, 1140
Tru9I TTAA 3 cut(s) 687, 1112, 1140
TscAI CASTG 3 cut(s) 50, 349, 496
TseFI GTSAC 2 cut(s) 340, 779
TseI GCWGC 5 cut(s) 13, 542, 626, 812, 1133
Tsp45I GTSAC 2 cut(s) 340, 779
TspDTI ATGAA 3 cut(s) 215, 521, 1193
TspGWI ACGGA 2 cut(s) 52, 518
TspRI CASTG 3 cut(s) 50, 349, 496
Tth111I GACNNNGTC 1 cut(s) 789
Vha464I CTTAAG 1 cut(s) 686
VneI GTGCAC 1 cut(s) 1056
VpaK11BI GGWCC 1 cut(s) 1262
XapI RAATTY 1 cut(s) 1253
XceI RCATGY 1 cut(s) 199
XhoI CTCGAG 1 cut(s) 269
XmiI GTMKAC 1 cut(s) 495
XmnI GAANNNNTTC 1 cut(s) 32
XspI CTAG 4 cut(s) 138, 656, 852, 1196
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.