pycom04g07300

Heat shock chaperonin-binding motif.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0013195
Physical Location & Seq
Reverse (-)
7740616 .. 7745838
5223 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g07300.1

Sequence Viewer

Length: 1290 bp
ATGGACGAGGCGAAGCTCAACCAACTCAAGCATTTCGTCGAACAGTGCAAATCCGATCCCTCCGTCCTCGCCGATCCTTCGCTCTCCTTCTTCCGCGACTACCTCGAGAGTCTCGGCGCTACGCTCCCTGCCTCTGCTCAGAAGCAAGGCGATTCAAAATCGAAGAGCTATGTCGTGGAGGAGAGCGACGACGACATGGCGGACACTGAAGCCGCTCAAGTTCATGTCGAAGAAGAGGAGGATGAGATAATCGAATCTGATGTTGAGCTCGAGGGTGACACCGTGGAGCCTGACGATGAACCTCCTCAGAAGATGGGAGACGCATCGGTTGAGGTGACTGATGAGAACCGTGATGCTGCACAGGCAGCAAAATCTAAAGCTATGGAGGCCATTTCAGAAGGTAACTTGGAGGAAGCAGTTGAACATCTCACTGAGGCGATTTTGCTCAATCCTACATCAGCAATTATGTACGGCACCAGAGCTAGTGTGTACATCAAAATGAAAAAACCTAGTGCTGCTATCCGGGATGCCAGTGCTGCTCTTGAGATCAATCCCGACTCTGCTAAAGGCTACAAGTCTCGTGGCATAGCACGAGCAATGCTTGGTCATTGGGAAGAGGCTGCTAAGGATCTTCACTTGGCATCAAAGCTTGATTATGATGAAGAAATAGGTGCTGTACTTAAGAAGGTTGAACCAAATGTGCACAGGATTGAGGAACATCGTCGCAAATATGATAGGCTTCGCAAAGAAAGAGAGGACAAAAGGATTGAGCGCGAGAGACAACGTCGCCGTGCTGAAGCTCAGGCTGCATATGAGAAGGCTAAGAAGCAAGAGCAGTCATCTTCTAGTAGAAGGTCTGGAGGCATGCCTGGTGGCTTTCCAGCGGGCATGGGTGGAGGCTTTCCAGGAGGAATGCCTGGTATGCCCGGTGGCTTTCCAGGAGGAATGCCTGGTATGCCCGGTGGCTTTCCAGCAGGCATGGGTGGAGGGTTTCCAGGAGGAATGCCTGGAGGGTTTCCAGCCGGCATGGGTGGCGGCTTTCCTGGAGGTGCATCTGGAGATGCACCTGGAGGTTTCCCCGGTAGCGCGCCAGGAGGGGTGCCTGGAAATGTTGATTTTAGCAAAATATTGAATGACCCTGAACTGATGAATGCATTTAGTGATCCAGAAGTCATGGATGCTCTTCAAGACGTAATGAAGAACCCTGCTAATCTAGCAAAGCATCAAGCAAATCCAAAGGTGGCTCCTATTATTGCAAAGATGATGAGCAAATTTGCCGGACCAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0008150 GO:0010035 GO:0010038 GO:0042221 GO:0044424 GO:0044444 GO:0044464 GO:0046686 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPR_8

Protein Analysis

430

Amino Acids

45.7

Weight (kDa)

4.97

Isoelectric Point (pI)

61.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HipN PF18253 2 - 43 9.8e-19 Hsp70-interacting protein N N-terminal domain
STI1-HOP_DP PF17830 373 - 423 1.5e-11 STI1/HOP, DP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013195)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G22670
fragaria_vesca FvH4_5g00470 FvH4_5g00470
malus_domestica MD04G1080700.v1.1
prunus_persica Prupe.5G077400_v2.0.a1
pyrus_communis pycom04g07300
rosa_chinensis RchiOBHm_Chr7g0202041
rosa_laevigata RLG00000003631
rosa_multiflora Rmu_sc0006592.1_g000004
rosa_roxburghii Rroxscaffold_3G00254910
rosa_rugosa Rorug07G0067500 Rorug07G0067600 Rorug07G0067700
rosa_samantha Rh7AG194800 Rh7BG196300 Rh7CG206100 Rh7DG201500
rosa_wichuraiana Rw7G017070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 473, 1099
AccBSI CCGCTC 1 cut(s) 215
AccII CGCG 3 cut(s) 96, 774, 1088
AciI CCGC 5 cut(s) 94, 200, 213, 884, 1035
AclWI GGATC 4 cut(s) 50, 68, 636, 1157
AcsI RAATTY 1 cut(s) 1271
AcuI CTGAAG 2 cut(s) 228, 816
AfaI GTAC 3 cut(s) 470, 491, 678
AflII CTTAAG 1 cut(s) 680
AgsI TTSAA 5 cut(s) 156, 422, 692, 1132, 1187
AluBI AGCT 7 cut(s) 16, 168, 268, 380, 482, 649, 800
AluI AGCT 7 cut(s) 16, 168, 268, 380, 482, 649, 800
Alw21I GWGCWC 2 cut(s) 270, 705
Alw26I GTCTC 4 cut(s) 116, 312, 582, 772
Alw44I GTGCAC 1 cut(s) 701
AlwI GGATC 4 cut(s) 50, 68, 636, 1157
Ama87I CYCGRG 2 cut(s) 104, 269
AoxI GGCC 1 cut(s) 387
ApaLI GTGCAC 1 cut(s) 701
ApeKI GCWGC 6 cut(s) 356, 365, 515, 536, 620, 806
ApoI RAATTY 1 cut(s) 1271
AspLEI GCGC 4 cut(s) 119, 774, 1088, 1090
AspS9I GGNCC 1 cut(s) 1280
AsuC2I CCSGG 4 cut(s) 524, 927, 960, 1080
AsuHPI GGTGA 2 cut(s) 287, 346
AvaI CYCGRG 2 cut(s) 104, 269
AvaII GGWCC 1 cut(s) 1280
BaeGI GKGCMC 1 cut(s) 705
BanI GGYRCC 2 cut(s) 473, 1099
BanII GRGCYC 1 cut(s) 270
BauI CACGAG 2 cut(s) 579, 591
Bbv12I GWGCWC 2 cut(s) 270, 705
BbvI GCAGC 6 cut(s) 343, 377, 502, 523, 607, 793
BccI CCATC 1 cut(s) 307
BceAI ACGGC 2 cut(s) 487, 774
BcnI CCSGG 4 cut(s) 524, 927, 960, 1080
BcoDI GTCTC 4 cut(s) 116, 312, 582, 772
BfaI CTAG 4 cut(s) 483, 510, 846, 1214
BfoI RGCGCY 1 cut(s) 120
BfrI CTTAAG 1 cut(s) 680
BisI GCNGC 8 cut(s) 213, 357, 366, 516, 537, 621, 807, 1036
BlsI GCNGC 8 cut(s) 214, 358, 367, 517, 538, 622, 808, 1037
Bme18I GGWCC 1 cut(s) 1280
BmeT110I CYCGRG 2 cut(s) 104, 269
BmgT120I GGNCC 1 cut(s) 1280
BmiI GGNNCC 4 cut(s) 288, 475, 1101, 1245
BmsI GCATC 8 cut(s) 332, 343, 517, 650, 1051, 1061, 1168, 1231
BpmI CTGGAG 5 cut(s) 879, 1029, 1065, 1077, 1089
Bpu10I CCTNAGC 2 cut(s) 624, 801
BpuEI CTTGAG 3 cut(s) 11, 201, 563
BpuMI CCSGG 4 cut(s) 524, 927, 960, 1080
BsaJI CCNNGG 2 cut(s) 282, 1078
Bse118I RCCGGY 1 cut(s) 1022
Bse1I ACTGG 1 cut(s) 531
Bse3DI GCAATG 1 cut(s) 603
BseDI CCNNGG 2 cut(s) 282, 1078
BseGI GGATG 3 cut(s) 247, 532, 1183
BseMI GCAATG 1 cut(s) 603
BseMII CTCAG 4 cut(s) 152, 320, 423, 815
BseNI ACTGG 1 cut(s) 531
BsePI GCGCGC 1 cut(s) 1086
BseRI GAGGAG 3 cut(s) 194, 251, 294
BseSI GKGCMC 1 cut(s) 705
BseXI GCAGC 6 cut(s) 343, 377, 502, 523, 607, 793
BsgI GTGCAG 1 cut(s) 342
Bsh1236I CGCG 3 cut(s) 96, 774, 1088
BshFI GGCC 1 cut(s) 389
BshNI GGYRCC 2 cut(s) 473, 1099
BsiHKAI GWGCWC 2 cut(s) 270, 705
BsiHKCI CYCGRG 2 cut(s) 104, 269
BsiSI CCGG 6 cut(s) 523, 927, 960, 1023, 1080, 1278
BsmAI GTCTC 4 cut(s) 116, 312, 582, 772
BsmBI CGTCTC 1 cut(s) 312
BsmI GAATGC 4 cut(s) 918, 951, 1008, 1156
BsnI GGCC 1 cut(s) 389
BsoBI CYCGRG 2 cut(s) 104, 269
Bsp1286I GDGCHC 2 cut(s) 270, 705
Bsp1407I TGTACA 1 cut(s) 489
Bsp143I GATC 5 cut(s) 55, 73, 546, 628, 1162
BspACI CCGC 5 cut(s) 94, 200, 213, 884, 1035
BspANI GGCC 1 cut(s) 389
BspCNI CTCAG 4 cut(s) 151, 319, 424, 814
BspFNI CGCG 3 cut(s) 96, 774, 1088
BspLI GGNNCC 4 cut(s) 288, 475, 1101, 1245
BspPI GGATC 4 cut(s) 50, 68, 636, 1157
BspQI GCTCTTC 2 cut(s) 158, 1188
BspT107I GGYRCC 2 cut(s) 473, 1099
BspTI CTTAAG 1 cut(s) 680
BsrBI CCGCTC 1 cut(s) 215
BsrDI GCAATG 1 cut(s) 603
BsrFI RCCGGY 1 cut(s) 1022
BsrGI TGTACA 1 cut(s) 489
BsrI ACTGG 1 cut(s) 531
BssAI RCCGGY 1 cut(s) 1022
BssECI CCNNGG 2 cut(s) 282, 1078
BssHII GCGCGC 1 cut(s) 1086
BssMI GATC 5 cut(s) 55, 73, 546, 628, 1162
BssSI CACGAG 2 cut(s) 579, 591
Bst2BI CACGAG 2 cut(s) 579, 591
Bst4CI ACNGT 3 cut(s) 45, 283, 350
Bst6I CTCTTC 4 cut(s) 158, 228, 609, 1188
BstAFI CTTAAG 1 cut(s) 680
BstAUI TGTACA 1 cut(s) 489
BstC8I GCNNGC 5 cut(s) 866, 886, 976, 1024, 1088
BstDEI CTNAG 6 cut(s) 138, 306, 432, 624, 801, 822
BstDSI CCRYGG 1 cut(s) 282
BstF5I GGATG 3 cut(s) 247, 532, 1183
BstFNI CGCG 3 cut(s) 96, 774, 1088
BstH2I RGCGCY 1 cut(s) 120
BstHHI GCGC 4 cut(s) 119, 774, 1088, 1090
BstKTI GATC 5 cut(s) 58, 76, 549, 631, 1165
BstMAI GTCTC 4 cut(s) 116, 312, 582, 772
BstMBI GATC 5 cut(s) 55, 73, 546, 628, 1162
BstMWI GCNNNNNNNGC 9 cut(s) 362, 365, 386, 536, 806, 922, 955, 1032, 1214
BstNSI RCATGY 1 cut(s) 868
BstSLI GKGCMC 1 cut(s) 705
BstUI CGCG 3 cut(s) 96, 774, 1088
BstV1I GCAGC 6 cut(s) 343, 377, 502, 523, 607, 793
BstX2I RGATCY 1 cut(s) 628
BstYI RGATCY 1 cut(s) 628
BsuRI GGCC 1 cut(s) 389
BtgI CCRYGG 1 cut(s) 282
BtsCI GGATG 3 cut(s) 247, 532, 1183
BtsIMutI CAGTG 4 cut(s) 50, 204, 429, 538
Cac8I GCNNGC 5 cut(s) 866, 886, 976, 1024, 1088
CfoI GCGC 4 cut(s) 119, 774, 1088, 1090
Cfr10I RCCGGY 1 cut(s) 1022
Cfr13I GGNCC 1 cut(s) 1280
CseI GACGC 1 cut(s) 329
Csp6I GTAC 3 cut(s) 469, 490, 677
CspCI CAANNNNNGTGG 2 cut(s) 562, 597
CviAII CATG 7 cut(s) 196, 224, 865, 889, 979, 1027, 1174
CviQI GTAC 3 cut(s) 469, 490, 677
DdeI CTNAG 6 cut(s) 138, 306, 432, 624, 801, 822
DpnI GATC 5 cut(s) 57, 75, 548, 630, 1164
DpnII GATC 5 cut(s) 55, 73, 546, 628, 1162
Eam1104I CTCTTC 4 cut(s) 158, 228, 609, 1188
EarI CTCTTC 4 cut(s) 158, 228, 609, 1188
EciI GGCGGA 1 cut(s) 215
Ecl136II GAGCTC 1 cut(s) 268
Eco24I GRGCYC 1 cut(s) 270
Eco47I GGWCC 1 cut(s) 1280
Eco53kI GAGCTC 1 cut(s) 268
Eco57I CTGAAG 2 cut(s) 228, 816
Eco88I CYCGRG 2 cut(s) 104, 269
EcoICRI GAGCTC 1 cut(s) 268
EcoT22I ATGCAT 1 cut(s) 1156
EcoT38I GRGCYC 1 cut(s) 270
Esp3I CGTCTC 1 cut(s) 312
FaeI CATG 7 cut(s) 199, 227, 868, 892, 982, 1030, 1177
FatI CATG 7 cut(s) 195, 223, 864, 888, 978, 1026, 1173
FauI CCCGC 1 cut(s) 877
FauNDI CATATG 1 cut(s) 811
Fnu4HI GCNGC 8 cut(s) 213, 357, 366, 516, 537, 621, 807, 1036
FokI GGATG 3 cut(s) 254, 539, 1190
FriOI GRGCYC 1 cut(s) 270
Fsp4HI GCNGC 8 cut(s) 213, 357, 366, 516, 537, 621, 807, 1036
FspBI CTAG 4 cut(s) 483, 510, 846, 1214
GlaI GCGC 4 cut(s) 118, 773, 1087, 1089
GluI GCNGC 8 cut(s) 213, 357, 366, 516, 537, 621, 807, 1036
GsuI CTGGAG 5 cut(s) 879, 1029, 1065, 1077, 1089
HaeII RGCGCY 1 cut(s) 120
HaeIII GGCC 1 cut(s) 389
HapII CCGG 6 cut(s) 523, 927, 960, 1023, 1080, 1278
HgaI GACGC 1 cut(s) 329
HhaI GCGC 4 cut(s) 119, 774, 1088, 1090
Hin1II CATG 7 cut(s) 199, 227, 868, 892, 982, 1030, 1177
Hin6I GCGC 4 cut(s) 117, 772, 1086, 1088
HinP1I GCGC 4 cut(s) 117, 772, 1086, 1088
HindIII AAGCTT 1 cut(s) 647
HinfI GANTC 4 cut(s) 109, 152, 254, 557
HpaII CCGG 6 cut(s) 523, 927, 960, 1023, 1080, 1278
HphI GGTGA 2 cut(s) 287, 346
Hpy166II GTNNAC 2 cut(s) 490, 703
Hpy188I TCNGA 5 cut(s) 55, 141, 259, 309, 397
Hpy188III TCNNGA 7 cut(s) 106, 542, 554, 858, 1056, 1166, 1187
Hpy8I GTNNAC 2 cut(s) 490, 703
Hpy99I CGWCG 5 cut(s) 41, 191, 194, 726, 789
HpyAV CCTTC 6 cut(s) 87, 97, 392, 679, 811, 846
HpyCH4III ACNGT 3 cut(s) 45, 283, 350
HpyCH4IV ACGT 2 cut(s) 784, 1191
HpyCH4V TGCA 8 cut(s) 48, 359, 703, 809, 1052, 1064, 1154, 1256
HpyF10VI GCNNNNNNNGC 9 cut(s) 362, 365, 386, 536, 806, 922, 955, 1032, 1214
HpyF3I CTNAG 6 cut(s) 138, 306, 432, 624, 801, 822
HpySE526I ACGT 2 cut(s) 784, 1191
Hsp92II CATG 7 cut(s) 199, 227, 868, 892, 982, 1030, 1177
HspAI GCGC 4 cut(s) 117, 772, 1086, 1088
KroI GCCGGC 1 cut(s) 1022
KroNI GCCGGC 1 cut(s) 1024
Kzo9I GATC 5 cut(s) 55, 73, 546, 628, 1162
LguI GCTCTTC 2 cut(s) 158, 1188
LmnI GCTCC 3 cut(s) 129, 286, 1249
Lsp1109I GCAGC 6 cut(s) 343, 377, 502, 523, 607, 793
LweI GCATC 8 cut(s) 332, 343, 517, 650, 1051, 1061, 1168, 1231
MaeI CTAG 4 cut(s) 483, 510, 846, 1214
MaeII ACGT 2 cut(s) 784, 1191
MaeIII GTNAC 3 cut(s) 275, 334, 401
MalI GATC 5 cut(s) 57, 75, 548, 630, 1164
MbiI CCGCTC 1 cut(s) 215
MboI GATC 5 cut(s) 55, 73, 546, 628, 1162
MflI RGATCY 1 cut(s) 628
MhlI GDGCHC 2 cut(s) 270, 705
MluCI AATT 2 cut(s) 462, 1271
MlyI GAGTC 2 cut(s) 118, 551
Mph1103I ATGCAT 1 cut(s) 1156
MroNI GCCGGC 1 cut(s) 1022
MseI TTAA 1 cut(s) 681
MslI CAYNNNNRTG 1 cut(s) 497
MspA1I CMGCKG 1 cut(s) 884
MspCI CTTAAG 1 cut(s) 680
MspI CCGG 6 cut(s) 523, 927, 960, 1023, 1080, 1278
Mva1269I GAATGC 4 cut(s) 918, 951, 1008, 1156
MvnI CGCG 3 cut(s) 96, 774, 1088
MwoI GCNNNNNNNGC 9 cut(s) 362, 365, 386, 536, 806, 922, 955, 1032, 1214
NaeI GCCGGC 1 cut(s) 1024
NciI CCSGG 4 cut(s) 524, 927, 960, 1080
NdeI CATATG 1 cut(s) 811
NdeII GATC 5 cut(s) 55, 73, 546, 628, 1162
NgoMIV GCCGGC 1 cut(s) 1022
NlaIII CATG 7 cut(s) 199, 227, 868, 892, 982, 1030, 1177
NlaIV GGNNCC 4 cut(s) 288, 475, 1101, 1245
NmeAIII GCCGAG 1 cut(s) 93
NmuCI GTSAC 2 cut(s) 275, 334
NsiI ATGCAT 1 cut(s) 1156
NspI RCATGY 1 cut(s) 868
PaeI GCATGC 1 cut(s) 868
PaeR7I CTCGAG 2 cut(s) 104, 269
PauI GCGCGC 1 cut(s) 1086
PciSI GCTCTTC 2 cut(s) 158, 1188
PctI GAATGC 4 cut(s) 918, 951, 1008, 1156
PdiI GCCGGC 1 cut(s) 1024
PfeI GAWTC 2 cut(s) 152, 254
PflFI GACNNNGTC 1 cut(s) 783
PfoI TCCNGGA 5 cut(s) 522, 904, 937, 994, 1042
PkrI GCNGC 8 cut(s) 214, 358, 367, 517, 538, 622, 808, 1037
PleI GAGTC 2 cut(s) 117, 551
PpsI GAGTC 2 cut(s) 117, 551
Psp124BI GAGCTC 1 cut(s) 270
PspN4I GGNNCC 4 cut(s) 288, 475, 1101, 1245
PspPI GGNCC 1 cut(s) 1280
PspXI VCTCGAGB 1 cut(s) 269
PsuI RGATCY 1 cut(s) 628
PsyI GACNNNGTC 1 cut(s) 783
PteI GCGCGC 1 cut(s) 1086
RsaI GTAC 3 cut(s) 470, 491, 678
RsaNI GTAC 3 cut(s) 469, 490, 677
RseI CAYNNNNRTG 1 cut(s) 497
SacI GAGCTC 1 cut(s) 270
SapI GCTCTTC 2 cut(s) 158, 1188
SaqAI TTAA 1 cut(s) 681
SatI GCNGC 8 cut(s) 213, 357, 366, 516, 537, 621, 807, 1036
Sau3AI GATC 5 cut(s) 55, 73, 546, 628, 1162
Sau96I GGNCC 1 cut(s) 1280
SchI GAGTC 2 cut(s) 118, 551
SduI GDGCHC 2 cut(s) 270, 705
SfaNI GCATC 8 cut(s) 332, 343, 517, 650, 1051, 1061, 1168, 1231
Sfr274I CTCGAG 2 cut(s) 104, 269
SinI GGWCC 1 cut(s) 1280
SlaI CTCGAG 2 cut(s) 104, 269
SmiMI CAYNNNNRTG 1 cut(s) 497
SmlI CTYRAG 6 cut(s) 26, 104, 216, 269, 542, 680
SmoI CTYRAG 6 cut(s) 26, 104, 216, 269, 542, 680
SphI GCATGC 1 cut(s) 868
Sse9I AATT 2 cut(s) 462, 1271
SsiI CCGC 5 cut(s) 94, 200, 213, 884, 1035
SspI AATATT 1 cut(s) 1128
SspMI CTAG 4 cut(s) 483, 510, 846, 1214
SstI GAGCTC 1 cut(s) 270
TaaI ACNGT 3 cut(s) 45, 283, 350
TaiI ACGT 2 cut(s) 787, 1194
TaqI TCGA 6 cut(s) 39, 105, 161, 228, 252, 270
TasI AATT 2 cut(s) 462, 1271
TatI WGTACW 2 cut(s) 489, 676
TauI GCSGC 2 cut(s) 215, 1038
TfiI GAWTC 2 cut(s) 152, 254
Tru1I TTAA 1 cut(s) 681
Tru9I TTAA 1 cut(s) 681
TscAI CASTG 4 cut(s) 50, 211, 436, 538
TseFI GTSAC 2 cut(s) 275, 334
TseI GCWGC 6 cut(s) 356, 365, 515, 536, 620, 806
Tsp45I GTSAC 2 cut(s) 275, 334
TspDTI ATGAA 6 cut(s) 212, 312, 515, 675, 1163, 1211
TspGWI ACGGA 1 cut(s) 52
TspRI CASTG 4 cut(s) 50, 211, 436, 538
Tth111I GACNNNGTC 1 cut(s) 783
Vha464I CTTAAG 1 cut(s) 680
VneI GTGCAC 1 cut(s) 701
VpaK11BI GGWCC 1 cut(s) 1280
XapI RAATTY 1 cut(s) 1271
XceI RCATGY 1 cut(s) 868
XhoI CTCGAG 2 cut(s) 104, 269
XspI CTAG 4 cut(s) 483, 510, 846, 1214
Zsp2I ATGCAT 1 cut(s) 1156
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.