Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb5

Orthogroup ID

OG0011780

Physical Location & Seq

Genomic Coordinates Forward (+)

730765 .. 735244

Gene Structure 4480 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_5g01220.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1449 bp

ATGGACGATCCTACCCTACCTTCTGCCTCCTCTTCTTCTTCTTCTTCGCAGCTCAACCAGCCGCCGCCGGCGACGCCCCAACCGGAGACGCCGGCGCGGCACATAGAGCGAATGCTGAACGTCAACCACCACCACCGATCGCCGTCGCGGACAATCTACTCCGACCGGTTCATACCCAGCAGATCCGGCTCCAACTTCGGTTTGTTTGATATCAAATCGCCGACGAATTCTCAGTCCGACGAGACCTCCAGCGCCTACACCACGCTCCTACGCGCCGCCCTGTTCGGTCCCGACAGCGCCGGCGTGGTTCCTCCGGCGACGCCCAATCGCCGGGGGCTGATGAACCCGCCGAACCAGAACATCTTCCGGTTCAAGAGCGAGACGCGGCGGTCCATGCACTCGCTGCTGCCGTTCGGGTTTGATGACGACGTCGTCACCGGAGTTCATCACAGTCCGGTGAAGACGCCGAGGAAGGTTCCCAGGTCGCCGTATAAGGTTTTGGATGCACCGGCTTTGCAAGACGATTTCTATTTGAATCTGGTGGATTGGTCTTCGAATAATGTATTGGCTGTAGGGTTGGGGAACTGTGTTTATTTGTGGAATGCTTGCAGTAGTAAGGTAACTAAGTTATGCGACTTGGGGATTGATGACAGTGTCTGTTCGGTGGGCTGGGCGCAGCGGGGAACACATCTTGCTGTAGGAACCGGCAATGGGAAAGTCCAGATTTGGGATGCATCTCGCTGTAAGAGGGTAAGAACTATGGAGGGCCACCGTCTACGTGTTGGGGCATTGGCCTGGAGTTCGTCAATGTTGTCTTCTGGTAGCCGTGACAAGAGTATTCTTCAACGGGATATTCGTGCTCAGGAAGATTTTGTTAGCAAACTTTCTGGACATAAGTCAGAGGTTTGTGGACTGAAGTGGTCTTATGACAACCGTGAGTTAGCATCAGGCGGGAATGATAATAGACTTTTTGTTTGGAATCAACATTCAACTCAACCCGTCCTGAAGTACTGTGAGCATACTGCTGCCGTGAAAGCTATTGCCTGGTCTCCTCATCTTCATGGACTTCTCGCATCTGGGGGAGGAACTGCAGACCGATGTATCCGTTTCTGGAATACAACCACTAATTCTCACTTGAGCTGCATGGACACTGGAAGTCAGGTATGCAATCTTGTGTGGTCCAAAAATGTCAATGAGCTCGTCAGCACACATGGGTACTCCCAAAATCAGATAATAGTTTGGAGATATCCTACTATGTCCAAGCTGGCAACTCTCACGGGCCATACATATAGAGTTCTTTATCTTGCCATCTCACCTGACGGACAGACAATTGTCACAGGAGCAGGAGATGAAACACTTAGGTTCTGGAATGTGTTTCCTTCCCCTAAATCTCAGAATACGGGAAGTGAAATCGGAGCGTCATGTCTAGGCAGAACCATCATCAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0000904 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006275 GO:0006725 GO:0006807 GO:0007049 GO:0008150 GO:0008152 GO:0008361 GO:0009058 GO:0009059 GO:0009653 GO:0009825 GO:0009888 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010026 GO:0010073 GO:0010074 GO:0010090 GO:0010091 GO:0010492 GO:0010556 GO:0010557 GO:0010564 GO:0010604 GO:0016043 GO:0016049 GO:0019219 GO:0019222 GO:0019827 GO:0022402 GO:0030154 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0032535 GO:0032875 GO:0032877 GO:0032989 GO:0034641 GO:0034645 GO:0040007 GO:0042023 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044424 GO:0044464 GO:0044786 GO:0045740 GO:0045787 GO:0045935 GO:0046483 GO:0048468 GO:0048507 GO:0048518 GO:0048522 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051052 GO:0051054 GO:0051171 GO:0051173 GO:0051726 GO:0060255 GO:0065007 GO:0065008 GO:0071704 GO:0071840 GO:0080090 GO:0090066 GO:0090068 GO:0090304 GO:0090329 GO:0090558 GO:0090626 GO:0098727 GO:1901360 GO:1901576 GO:2000105 GO:2000112

KEGG Pathways

Metabolic & Signaling

ko04110 ko04111 ko04120 ko04914 map04110 map04111 map04120 map04914

Pfam Domains

Protein Families

ANAPC4_WD40 WD40

Protein Analysis

483

Amino Acids

53.03

Weight (kDa)

9.22

Isoelectric Point (pI)

48.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WD40_CDC20-Fz	PF24807	167 - 456	1.2e-127	CDC20/Fizzy WD40 domain
WD40_WDHD1_1st	PF24817	178 - 268	3.7e-08	WDHD1 first WD40 domain
Beta-prop_WDR19_1st	PF23389	182 - 280	7.6e-12	WDR19 first beta-propeller
Beta-prop_EML_2	PF23414	185 - 350	7.6e-17	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st	PF25173	188 - 289	3.9e-13	WDR3 first beta-propeller domain
Beta-prop_THOC3	PF25174	196 - 280	1.5e-09	THOC3 beta-propeller domain
WD40_Prp19	PF24814	208 - 289	5.9e-06	Prp19 WD40 domain
WDR55	PF24796	213 - 461	2.9e-11	WDR55
Beta-prop_WDR5	PF25175	216 - 382	2.4e-23	WDR5 beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	216 - 280	5.2e-06	WDR3 second beta-propeller domain
WD40_MABP1-WDR62_2nd	PF24782	218 - 457	1.5e-14	MABP1/WDR62 second WD40 domain
Beta-prop_TEP1_2nd	PF25047	219 - 392	3.6e-11	TEP-1 second beta-propeller
WD40_Prp19	PF24814	269 - 457	3.7e-17	Prp19 WD40 domain
Beta-prop_CAF1B_HIR1	PF24105	286 - 378	4.2e-10	CAF1B/HIR1 beta-propeller domain
WD40_WDHD1_1st	PF24817	287 - 355	2.4e-06	WDHD1 first WD40 domain
Beta-prop_WDR3_1st	PF25173	290 - 402	1.3e-14	WDR3 first beta-propeller domain
WD40	PF00400	291 - 327	4.1e-07	WD domain, G-beta repeat
Beta-prop_Aladin	PF25460	291 - 458	1.9e-08	Aladin seven-bladed propeller
WD40_Gbeta	PF25391	293 - 461	1.7e-12	G protein beta WD-40 repeat protein
Beta-prop_THOC3	PF25174	298 - 403	1e-18	THOC3 beta-propeller domain
Beta-prop_EML	PF23409	337 - 459	2.1e-10	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR36-Utp21_2nd	PF25168	340 - 458	1.5e-06	WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR5	PF25175	340 - 458	1.7e-13	WDR5 beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	364 - 458	4.1e-08	WDR3 second beta-propeller domain
Beta-prop_WDR3_1st	PF25173	387 - 458	2.3e-08	WDR3 first beta-propeller domain
Beta-prop_THOC3	PF25174	407 - 458	2.6e-08	THOC3 beta-propeller domain
WD40	PF00400	421 - 457	1e-07	WD domain, G-beta repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0011780)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G11920 AT4G22910
fragaria_vesca	FvH4_5g01220 FvH4_5g01220
malus_domestica	MD00G1131600.v1.1 MD04G1070400.v1.1 MD11G1180200.v1.1 MD13G1123600.v1.1
prunus_persica	Prupe.5G087300_v2.0.a1
pyrus_communis	pycom04g06380 pycom06g06850
rosa_chinensis	RchiOBHm_Chr7g0199871
rosa_laevigata	RLG00000003764
rosa_multiflora	Rmu_sc0002748.1_g000015
rosa_roxburghii	Rroxscaffold_3G00256420
rosa_rugosa	Rorug07G0056700
rosa_samantha	Rh7AG181900 Rh7CG191500
rosa_wichuraiana	Rw7G015960

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	388, 431
AatII	GACGTC	1 cut(s)	432
AccI	GTMKAC	1 cut(s)	775
AccII	CGCG	4 cut(s)	97, 148, 273, 385
AclWI	GGATC	2 cut(s)	2, 177
AcsI	RAATTY	1 cut(s)	226
AcuI	CTGAAG	2 cut(s)	935, 1025
AcyI	GRCGYC	5 cut(s)	74, 89, 320, 429, 464
AfaI	GTAC	2 cut(s)	1010, 1217
AfiI	CCNNNNNNNGG	3 cut(s)	330, 711, 727
AflIII	ACRYGT	1 cut(s)	778
AgeI	ACCGGT	1 cut(s)	165
AgsI	TTSAA	4 cut(s)	373, 535, 845, 990
AjnI	CCWGG	3 cut(s)	479, 794, 1043
AjuI	GAANNNNNNNTTGG	2 cut(s)	548, 580
AluBI	AGCT	5 cut(s)	52, 1037, 1140, 1198, 1264
AluI	AGCT	5 cut(s)	52, 1037, 1140, 1198, 1264
Alw21I	GWGCWC	2 cut(s)	862, 1200
Alw26I	GTCTC	4 cut(s)	80, 236, 374, 1053
AlwI	GGATC	2 cut(s)	2, 177
AlwNI	CAGNNNCTG	1 cut(s)	657
AoxI	GGCC	3 cut(s)	766, 792, 1279
ApeKI	GCWGC	6 cut(s)	49, 403, 406, 676, 1025, 1140
ApoI	RAATTY	1 cut(s)	226
ArsI	GACNNNNNNTTYG	4 cut(s)	218, 250, 957, 989
AsiGI	ACCGGT	1 cut(s)	165
Asp700I	GAANNNNTTC	1 cut(s)	362
AspLEI	GCGC	5 cut(s)	97, 254, 275, 299, 676
AspS9I	GGNCC	5 cut(s)	287, 390, 766, 1179, 1279
AsuC2I	CCSGG	1 cut(s)	332
AsuHPI	GGTGA	3 cut(s)	427, 469, 1305
AsuII	TTCGAA	1 cut(s)	554
AvaII	GGWCC	3 cut(s)	287, 390, 1179
BaeI	ACNNNNGTAYC	2 cut(s)	1084, 1117
BanII	GRGCYC	1 cut(s)	1200
BbsI	GAAGAC	3 cut(s)	467, 543, 807
Bbv12I	GWGCWC	2 cut(s)	862, 1200
BbvI	GCAGC	6 cut(s)	61, 390, 393, 688, 1012, 1127
BccI	CCATC	2 cut(s)	1316, 1445
BceAI	ACGGC	5 cut(s)	127, 394, 472, 810, 1013
BciT130I	CCWGG	3 cut(s)	481, 796, 1045
BciVI	GTATCC	1 cut(s)	1112
BcnI	CCSGG	1 cut(s)	332
BcoDI	GTCTC	4 cut(s)	80, 236, 374, 1053
BfaI	CTAG	1 cut(s)	1427
BfmI	CTRYAG	3 cut(s)	570, 696, 1089
BfoI	RGCGCY	2 cut(s)	255, 300
BfuI	GTATCC	1 cut(s)	1112
BglI	GCCNNNNNGGC	1 cut(s)	97
BmcAI	AGTACT	1 cut(s)	1010
Bme1390I	CCNGG	4 cut(s)	332, 481, 796, 1045
Bme18I	GGWCC	3 cut(s)	287, 390, 1179
BmgT120I	GGNCC	5 cut(s)	287, 390, 766, 1179, 1279
BmiI	GGNNCC	5 cut(s)	190, 289, 309, 477, 703
BmrFI	CCNGG	4 cut(s)	332, 481, 796, 1045
BmsI	GCATC	5 cut(s)	493, 721, 743, 953, 1082
BoxI	GACNNNNGTC	2 cut(s)	895, 1331
BpiI	GAAGAC	3 cut(s)	467, 543, 807
BpmI	CTGGAG	2 cut(s)	232, 817
Bpu10I	CCTNAGC	1 cut(s)	861
Bpu14I	TTCGAA	1 cut(s)	554
BpuEI	CTTGAG	1 cut(s)	1156
BpuMI	CCSGG	1 cut(s)	332
BsaAI	YACGTR	1 cut(s)	779
BsaHI	GRCGYC	5 cut(s)	74, 89, 320, 429, 464
BsaI	GGTCTC	2 cut(s)	236, 1053
BsaJI	CCNNGG	3 cut(s)	331, 467, 479
BsaWI	WCCGGW	5 cut(s)	82, 165, 366, 437, 454
BsaXI	ACNNNNNCTCC	2 cut(s)	230, 260
Bsc4I	CCNNNNNNNGG	3 cut(s)	330, 711, 727
Bse118I	RCCGGY	6 cut(s)	67, 91, 165, 299, 508, 704
Bse1I	ACTGG	1 cut(s)	1156
Bse3DI	GCAATG	1 cut(s)	715
BseBI	CCWGG	3 cut(s)	481, 796, 1045
BseDI	CCNNGG	3 cut(s)	331, 467, 479
BseGI	GGATG	2 cut(s)	508, 736
BseLI	CCNNNNNNNGG	3 cut(s)	330, 711, 727
BseMI	GCAATG	1 cut(s)	715
BseMII	CTCAG	3 cut(s)	245, 875, 1406
BseNI	ACTGG	1 cut(s)	1156
BseRI	GAGGAG	2 cut(s)	19, 1041
BseXI	GCAGC	6 cut(s)	61, 390, 393, 688, 1012, 1127
BseYI	CCCAGC	2 cut(s)	176, 669
Bsh1236I	CGCG	4 cut(s)	97, 148, 273, 385
Bsh1285I	CGRYCG	2 cut(s)	140, 166
BshFI	GGCC	3 cut(s)	768, 794, 1281
BshTI	ACCGGT	1 cut(s)	165
BsiEI	CGRYCG	2 cut(s)	140, 166
BsiHKAI	GWGCWC	2 cut(s)	862, 1200
BslFI	GGGAC	1 cut(s)	273
BslI	CCNNNNNNNGG	3 cut(s)	330, 711, 727
BsmAI	GTCTC	4 cut(s)	80, 236, 374, 1053
BsmBI	CGTCTC	2 cut(s)	80, 374
BsmFI	GGGAC	1 cut(s)	273
BsmI	GAATGC	2 cut(s)	117, 607
BsnI	GGCC	3 cut(s)	768, 794, 1281
Bso31I	GGTCTC	2 cut(s)	236, 1053
Bsp119I	TTCGAA	1 cut(s)	554
Bsp1286I	GDGCHC	2 cut(s)	862, 1200
Bsp143I	GATC	3 cut(s)	7, 137, 182
BspANI	GGCC	3 cut(s)	768, 794, 1281
BspCNI	CTCAG	3 cut(s)	244, 874, 1405
BspFNI	CGCG	4 cut(s)	97, 148, 273, 385
BspLI	GGNNCC	5 cut(s)	190, 289, 309, 477, 703
BspMAI	CTGCAG	1 cut(s)	1093
BspPI	GGATC	2 cut(s)	2, 177
BspT104I	TTCGAA	1 cut(s)	554
BspTNI	GGTCTC	2 cut(s)	236, 1053
BsrDI	GCAATG	1 cut(s)	715
BsrFI	RCCGGY	6 cut(s)	67, 91, 165, 299, 508, 704
BsrI	ACTGG	1 cut(s)	1156
BssAI	RCCGGY	6 cut(s)	67, 91, 165, 299, 508, 704
BssECI	CCNNGG	3 cut(s)	331, 467, 479
BssMI	GATC	3 cut(s)	7, 137, 182
BssNI	GRCGYC	5 cut(s)	74, 89, 320, 429, 464
Bst2UI	CCWGG	3 cut(s)	481, 796, 1045
Bst4CI	ACNGT	6 cut(s)	452, 587, 653, 773, 935, 1013
Bst6I	CTCTTC	1 cut(s)	37
BstACI	GRCGYC	5 cut(s)	74, 89, 320, 429, 464
BstAPI	GCANNNNNTGC	1 cut(s)	403
BstBAI	YACGTR	1 cut(s)	779
BstBI	TTCGAA	1 cut(s)	554
BstC8I	GCNNGC	5 cut(s)	69, 93, 301, 607, 1266
BstDEI	CTNAG	5 cut(s)	231, 624, 861, 1358, 1392
BstF5I	GGATG	2 cut(s)	508, 736
BstFNI	CGCG	4 cut(s)	97, 148, 273, 385
BstH2I	RGCGCY	2 cut(s)	255, 300
BstHHI	GCGC	5 cut(s)	97, 254, 275, 299, 676
BstKTI	GATC	3 cut(s)	10, 140, 185
BstMAI	GTCTC	4 cut(s)	80, 236, 374, 1053
BstMBI	GATC	3 cut(s)	7, 137, 182
BstMCI	CGRYCG	2 cut(s)	140, 166
BstMWI	GCNNNNNNNGC	8 cut(s)	58, 73, 97, 106, 186, 394, 403, 1034
BstNI	CCWGG	3 cut(s)	481, 796, 1045
BstPAI	GACNNNNGTC	2 cut(s)	895, 1331
BstSCI	CCNGG	4 cut(s)	330, 479, 794, 1043
BstSFI	CTRYAG	3 cut(s)	570, 696, 1089
BstUI	CGCG	4 cut(s)	97, 148, 273, 385
BstV1I	GCAGC	6 cut(s)	61, 390, 393, 688, 1012, 1127
BstV2I	GAAGAC	3 cut(s)	467, 543, 807
BstX2I	RGATCY	1 cut(s)	182
BstYI	RGATCY	1 cut(s)	182
BsuI	GTATCC	1 cut(s)	1112
BsuRI	GGCC	3 cut(s)	768, 794, 1281
BtsCI	GGATG	2 cut(s)	508, 736
BtsIMutI	CAGTG	2 cut(s)	658, 1149
Cac8I	GCNNGC	5 cut(s)	69, 93, 301, 607, 1266
CaiI	CAGNNNCTG	1 cut(s)	657
CfoI	GCGC	5 cut(s)	97, 254, 275, 299, 676
Cfr10I	RCCGGY	6 cut(s)	67, 91, 165, 299, 508, 704
Cfr13I	GGNCC	5 cut(s)	287, 390, 766, 1179, 1279
CseI	GACGC	6 cut(s)	82, 97, 328, 391, 472, 1407
Csp6I	GTAC	2 cut(s)	1009, 1216
CspAI	ACCGGT	1 cut(s)	165
CviAII	CATG	5 cut(s)	394, 1061, 1144, 1211, 1422
CviQI	GTAC	2 cut(s)	1009, 1216
DdeI	CTNAG	5 cut(s)	231, 624, 861, 1358, 1392
DpnI	GATC	3 cut(s)	9, 139, 184
DpnII	GATC	3 cut(s)	7, 137, 182
DrdI	GACNNNNNNGTC	2 cut(s)	388, 431
DseDI	GACNNNNNNGTC	2 cut(s)	388, 431
Eam1104I	CTCTTC	1 cut(s)	37
EarI	CTCTTC	1 cut(s)	37
Ecl136II	GAGCTC	1 cut(s)	1198
Eco24I	GRGCYC	1 cut(s)	1200
Eco31I	GGTCTC	2 cut(s)	236, 1053
Eco32I	GATATC	2 cut(s)	211, 1247
Eco47I	GGWCC	3 cut(s)	287, 390, 1179
Eco53kI	GAGCTC	1 cut(s)	1198
Eco57I	CTGAAG	2 cut(s)	935, 1025
EcoICRI	GAGCTC	1 cut(s)	1198
EcoRI	GAATTC	1 cut(s)	226
EcoRII	CCWGG	3 cut(s)	479, 794, 1043
EcoRV	GATATC	2 cut(s)	211, 1247
EcoT22I	ATGCAT	1 cut(s)	736
EcoT38I	GRGCYC	1 cut(s)	1200
Esp3I	CGTCTC	2 cut(s)	80, 374
FaeI	CATG	5 cut(s)	397, 1064, 1147, 1214, 1425
FaqI	GGGAC	1 cut(s)	273
FatI	CATG	5 cut(s)	393, 1060, 1143, 1210, 1421
FauI	CCCGC	3 cut(s)	354, 672, 944
FblI	GTMKAC	1 cut(s)	775
FokI	GGATG	2 cut(s)	515, 743
FriOI	GRGCYC	1 cut(s)	1200
FspBI	CTAG	1 cut(s)	1427
GlaI	GCGC	5 cut(s)	96, 253, 274, 298, 675
GsaI	CCCAGC	2 cut(s)	180, 673
GsuI	CTGGAG	2 cut(s)	232, 817
HaeII	RGCGCY	2 cut(s)	255, 300
HaeIII	GGCC	3 cut(s)	768, 794, 1281
HgaI	GACGC	6 cut(s)	82, 97, 328, 391, 472, 1407
HhaI	GCGC	5 cut(s)	97, 254, 275, 299, 676
Hin1I	GRCGYC	5 cut(s)	74, 89, 320, 429, 464
Hin1II	CATG	5 cut(s)	397, 1064, 1147, 1214, 1425
Hin6I	GCGC	5 cut(s)	95, 252, 273, 297, 674
HinP1I	GCGC	5 cut(s)	95, 252, 273, 297, 674
HincII	GTYRAC	1 cut(s)	124
HindII	GTYRAC	1 cut(s)	124
HinfI	GANTC	2 cut(s)	535, 979
HphI	GGTGA	3 cut(s)	427, 469, 1305
Hpy166II	GTNNAC	3 cut(s)	124, 776, 911
Hpy188I	TCNGA	7 cut(s)	163, 238, 901, 1230, 1395, 1415, 1445
Hpy188III	TCNNGA	8 cut(s)	290, 373, 721, 863, 888, 1003, 1111, 1366
Hpy8I	GTNNAC	3 cut(s)	124, 776, 911
Hpy99I	CGWCG	7 cut(s)	76, 148, 226, 242, 322, 431, 434
HpyAV	CCTTC	3 cut(s)	30, 466, 1389
HpyCH4III	ACNGT	6 cut(s)	452, 587, 653, 773, 935, 1013
HpyCH4IV	ACGT	3 cut(s)	120, 429, 778
HpyCH4V	TGCA	8 cut(s)	397, 506, 517, 609, 734, 1091, 1143, 1167
HpyF10VI	GCNNNNNNNGC	8 cut(s)	58, 73, 97, 106, 186, 394, 403, 1034
HpyF3I	CTNAG	5 cut(s)	231, 624, 861, 1358, 1392
HpySE526I	ACGT	3 cut(s)	120, 429, 778
Hsp92I	GRCGYC	5 cut(s)	74, 89, 320, 429, 464
Hsp92II	CATG	5 cut(s)	397, 1064, 1147, 1214, 1425
HspAI	GCGC	5 cut(s)	95, 252, 273, 297, 674
KroI	GCCGGC	3 cut(s)	67, 91, 299
KroNI	GCCGGC	3 cut(s)	69, 93, 301
Kzo9I	GATC	3 cut(s)	7, 137, 182
LmnI	GCTCC	4 cut(s)	194, 270, 1340, 1415
Lsp1109I	GCAGC	6 cut(s)	61, 390, 393, 688, 1012, 1127
LweI	GCATC	5 cut(s)	493, 721, 743, 953, 1082
MaeI	CTAG	1 cut(s)	1427
MaeII	ACGT	3 cut(s)	120, 429, 778
MaeIII	GTNAC	4 cut(s)	433, 619, 827, 1333
MalI	GATC	3 cut(s)	9, 139, 184
MboI	GATC	3 cut(s)	7, 137, 182
MfeI	CAATTG	1 cut(s)	1329
MflI	RGATCY	1 cut(s)	182
MhlI	GDGCHC	2 cut(s)	862, 1200
MluCI	AATT	3 cut(s)	226, 1126, 1329
MmeI	TCCRAC	3 cut(s)	186, 216, 261
Mph1103I	ATGCAT	1 cut(s)	736
MreI	CGCCGGCG	3 cut(s)	67, 91, 299
MroNI	GCCGGC	3 cut(s)	67, 91, 299
MroXI	GAANNNNTTC	1 cut(s)	362
MslI	CAYNNNNRTG	1 cut(s)	1059
MspA1I	CMGCKG	1 cut(s)	679
MspR9I	CCNGG	4 cut(s)	332, 481, 796, 1045
MunI	CAATTG	1 cut(s)	1329
Mva1269I	GAATGC	2 cut(s)	117, 607
MvaI	CCWGG	3 cut(s)	481, 796, 1045
MvnI	CGCG	4 cut(s)	97, 148, 273, 385
MwoI	GCNNNNNNNGC	8 cut(s)	58, 73, 97, 106, 186, 394, 403, 1034
NaeI	GCCGGC	3 cut(s)	69, 93, 301
NciI	CCSGG	1 cut(s)	332
NdeII	GATC	3 cut(s)	7, 137, 182
NgoMIV	GCCGGC	3 cut(s)	67, 91, 299
NlaIII	CATG	5 cut(s)	397, 1064, 1147, 1214, 1425
NlaIV	GGNNCC	5 cut(s)	190, 289, 309, 477, 703
NmeAIII	GCCGAG	1 cut(s)	492
NmuCI	GTSAC	3 cut(s)	433, 827, 1333
NsiI	ATGCAT	1 cut(s)	736
NspV	TTCGAA	1 cut(s)	554
PcsI	WCGNNNNNNNCGW	2 cut(s)	407, 853
PctI	GAATGC	2 cut(s)	117, 607
PdiI	GCCGGC	3 cut(s)	69, 93, 301
PdmI	GAANNNNTTC	1 cut(s)	362
PfeI	GAWTC	2 cut(s)	535, 979
PflFI	GACNNNGTC	3 cut(s)	428, 431, 653
PinAI	ACCGGT	1 cut(s)	165
Ple19I	CGATCG	1 cut(s)	140
Ppu21I	YACGTR	1 cut(s)	779
PshAI	GACNNNNGTC	2 cut(s)	895, 1331
Psp124BI	GAGCTC	1 cut(s)	1200
Psp6I	CCWGG	3 cut(s)	479, 794, 1043
PspFI	CCCAGC	2 cut(s)	176, 669
PspGI	CCWGG	3 cut(s)	479, 794, 1043
PspN4I	GGNNCC	5 cut(s)	190, 289, 309, 477, 703
PspPI	GGNCC	5 cut(s)	287, 390, 766, 1179, 1279
PstI	CTGCAG	1 cut(s)	1093
PstNI	CAGNNNCTG	1 cut(s)	657
PsuI	RGATCY	1 cut(s)	182
PsyI	GACNNNGTC	3 cut(s)	428, 431, 653
PvuI	CGATCG	1 cut(s)	140
RsaI	GTAC	2 cut(s)	1010, 1217
RsaNI	GTAC	2 cut(s)	1009, 1216
RseI	CAYNNNNRTG	1 cut(s)	1059
SacI	GAGCTC	1 cut(s)	1200
Sau3AI	GATC	3 cut(s)	7, 137, 182
Sau96I	GGNCC	5 cut(s)	287, 390, 766, 1179, 1279
ScaI	AGTACT	1 cut(s)	1010
ScrFI	CCNGG	4 cut(s)	332, 481, 796, 1045
SduI	GDGCHC	2 cut(s)	862, 1200
SfaNI	GCATC	5 cut(s)	493, 721, 743, 953, 1082
SfcI	CTRYAG	3 cut(s)	570, 696, 1089
SfuI	TTCGAA	1 cut(s)	554
SgrAI	CRCCGGYG	3 cut(s)	67, 91, 299
SinI	GGWCC	3 cut(s)	287, 390, 1179
SmiMI	CAYNNNNRTG	1 cut(s)	1059
SmlI	CTYRAG	1 cut(s)	1135
SmoI	CTYRAG	1 cut(s)	1135
Sse9I	AATT	3 cut(s)	226, 1126, 1329
SspMI	CTAG	1 cut(s)	1427
SstI	GAGCTC	1 cut(s)	1200
StyD4I	CCNGG	4 cut(s)	330, 479, 794, 1043
TaaI	ACNGT	6 cut(s)	452, 587, 653, 773, 935, 1013
TaiI	ACGT	3 cut(s)	123, 432, 781
TaqI	TCGA	1 cut(s)	554
TaqII	GACCGA	2 cut(s)	275, 1110
TasI	AATT	3 cut(s)	226, 1126, 1329
TatI	WGTACW	1 cut(s)	1008
TauI	GCSGC	5 cut(s)	64, 67, 100, 278, 388
TfiI	GAWTC	2 cut(s)	535, 979
TscAI	CASTG	2 cut(s)	658, 1156
TseFI	GTSAC	3 cut(s)	433, 827, 1333
TseI	GCWGC	6 cut(s)	49, 403, 406, 676, 1025, 1140
Tsp45I	GTSAC	3 cut(s)	433, 827, 1333
TspDTI	ATGAA	5 cut(s)	160, 356, 434, 1049, 1365
TspGWI	ACGGA	2 cut(s)	1094, 1335
TspRI	CASTG	2 cut(s)	658, 1156
Tth111I	GACNNNGTC	3 cut(s)	428, 431, 653
VpaK11BI	GGWCC	3 cut(s)	287, 390, 1179
XapI	RAATTY	1 cut(s)	226
XmiI	GTMKAC	1 cut(s)	775
XmnI	GAANNNNTTC	1 cut(s)	362
XspI	CTAG	1 cut(s)	1427
ZraI	GACGTC	1 cut(s)	430
ZrmI	AGTACT	1 cut(s)	1010
Zsp2I	ATGCAT	1 cut(s)	736

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.