Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0011780

Physical Location & Seq

Genomic Coordinates Forward (+)

52408497 .. 52412394

Gene Structure 3898 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000003764

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1461 bp

ATGGACGATCCTATGCTATCTTCTTCTTCTTCTTCTTCTACGCAGCCCAATCAGCCGCCGCCGACGCTGCCGCGGACCCCTCTCGCGGTCCAGCTGGAGACGCCGGCCCGGCACATCGAGCGAATGCTGAACGTCAACCACCACCACCGATCGCCGTCGCGGACCATCTACTCCGATAGGTTCATACCCAGCAGATCCGGCTCTAACTTTGGTTTGTTCGACATCAAATCGCCGACGAATTCTCAGTCCGACGAGACCTCCAGCGCCTACACCACGCTCTTACGCGCCGCCCTCTTCGGCCCCGACACCGCCGGCGTGATTCCTCCGGCGACCCCCGATCGGCGGGGGCTGATGAACCCGCCGAACCAGAATATATTCCGGTTCAAGAGCGAGACCCGGCGGTCCATGCACTCGCTGCTGCCGTTTGGGTTTGATGATGACGTGGTCACCGGAGTTCATCATAGTCCGGTGAAGACCCCCAGGAAGGTCCCCAGATCGCCCTACAAGGTTTTGGATGCACCGGCTTTGCAAGACGATTTCTATCTGAATCTGGTGGATTGGTCTTCGCATAATGTGTTGGCTGTGGGGTTGGGGAACTGTGTTTATTTGTGGAATGCTTGTAGTAGTAAGGTAACTAAGTTATGCGATTTGGGGACTGATGATAGTGTCTGTTCGGTTGGCTGGGCGCAGCGGGGAACTCATCTTGCTGTGGGAACTGGCAATGGGAAAGTCCAAATTTGGGATGCATCTCGCTGTAAGAGGGTAAGAACTATGGAGGGCCATCGTCTACGTATTGGGGCATTGGCCTGGAGTTCATCCATGTTGTCTTCTGGTAGCCGTGACAAGAGTATTCTTCAACGGGATATACGCGCTCAGGAAGATTTTGTTAGCAAACTTTCTGGACATAAGTCAGAGGTTTGTGGACTGAAGTGGTCTTATGACAACCGTGAGTTGGCATCAGGCGGGAATGATAATAGACTTTTTGTTTGGAATCAACATTCAACTCAACCCGTGCTGAAATACTGTGAGCATACTGCTGCCGTGAAAGCTATTGCATGGTCCCCCCATCTTCATGGACTTCTTGCATCTGGGGGAGGAACTGCAGACCGATGTATCCGTTTCTGGAATACAACCACTAACTCTCACTTGAGCTGCATGGACACTGGAAGTCAGGTTTGCAATCTTGTGTGGTCCAAAAATGTCAATGAGCTCGTCAGCACACACGGGTACTCCCAAAATCAGATAATAGTTTGGAGATATCCTACGATGTCCAAGCTGGCAACTCTCACGGGCCATACATATAGAGTTCTTTATCTTGCCATCTCACCTGACGGACAGACAATTGTCACAGGAGCAGGAGATGAAACACTTAGGTTTTGGAATGTGTTTCCTTCCCCTAAATCTCAGAATACGGGAAGTGAAATCGGAGCATCATGTCTAGGCAGAACCATCATCAGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0000904 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006275 GO:0006725 GO:0006807 GO:0007049 GO:0008150 GO:0008152 GO:0008361 GO:0009058 GO:0009059 GO:0009653 GO:0009825 GO:0009888 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010026 GO:0010073 GO:0010074 GO:0010090 GO:0010091 GO:0010492 GO:0010556 GO:0010557 GO:0010564 GO:0010604 GO:0016043 GO:0016049 GO:0019219 GO:0019222 GO:0019827 GO:0022402 GO:0030154 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0032535 GO:0032875 GO:0032877 GO:0032989 GO:0034641 GO:0034645 GO:0040007 GO:0042023 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044424 GO:0044464 GO:0044786 GO:0045740 GO:0045787 GO:0045935 GO:0046483 GO:0048468 GO:0048507 GO:0048518 GO:0048522 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051052 GO:0051054 GO:0051171 GO:0051173 GO:0051726 GO:0060255 GO:0065007 GO:0065008 GO:0071704 GO:0071840 GO:0080090 GO:0090066 GO:0090068 GO:0090304 GO:0090329 GO:0090558 GO:0090626 GO:0098727 GO:1901360 GO:1901576 GO:2000105 GO:2000112

KEGG Pathways

Metabolic & Signaling

ko04110 ko04111 ko04120 ko04914 map04110 map04111 map04120 map04914

Pfam Domains

Protein Families

ANAPC4_WD40 WD40

Protein Analysis

487

Amino Acids

53.63

Weight (kDa)

9.22

Isoelectric Point (pI)

45.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WD40_CDC20-Fz	PF24807	171 - 460	8.2e-128	CDC20/Fizzy WD40 domain
WD40_WDHD1_1st	PF24817	183 - 272	2.1e-08	WDHD1 first WD40 domain
Beta-prop_EML_2	PF23414	189 - 354	4.9e-17	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR19_1st	PF23389	191 - 284	4.5e-12	WDR19 first beta-propeller
Beta-prop_WDR3_1st	PF25173	192 - 293	2.9e-13	WDR3 first beta-propeller domain
Beta-prop_THOC3	PF25174	200 - 284	2.9e-09	THOC3 beta-propeller domain
ANAPC4_WD40	PF12894	211 - 272	9.3e-06	Anaphase-promoting complex subunit 4 WD40 domain
Beta-prop_WDR5	PF25175	219 - 400	1.5e-23	WDR5 beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	219 - 284	6.4e-06	WDR3 second beta-propeller domain
WDR55	PF24796	219 - 465	3e-11	WDR55
WD40_MABP1-WDR62_2nd	PF24782	221 - 461	1.7e-14	MABP1/WDR62 second WD40 domain
Beta-prop_TEP1_2nd	PF25047	223 - 395	2.6e-10	TEP-1 second beta-propeller
WD40_Prp19	PF24814	273 - 461	3.3e-17	Prp19 WD40 domain
Beta-prop_CAF1B_HIR1	PF24105	290 - 382	4.2e-10	CAF1B/HIR1 beta-propeller domain
WD40_WDHD1_1st	PF24817	291 - 359	2.5e-06	WDHD1 first WD40 domain
Beta-prop_WDR3_1st	PF25173	294 - 406	1.4e-14	WDR3 first beta-propeller domain
WD40	PF00400	295 - 331	4.3e-07	WD domain, G-beta repeat
WD40_Gbeta	PF25391	297 - 466	1.9e-12	G protein beta WD-40 repeat protein
Beta-prop_THOC3	PF25174	302 - 408	7.7e-19	THOC3 beta-propeller domain
Beta-prop_Aladin	PF25460	302 - 462	2.5e-08	Aladin seven-bladed propeller
Beta-prop_EML	PF23409	341 - 463	2.6e-10	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR5	PF25175	343 - 462	1.1e-13	WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd	PF25168	344 - 462	1.6e-06	WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	368 - 465	2.5e-08	WDR3 second beta-propeller domain
Beta-prop_WDR3_1st	PF25173	391 - 462	2.4e-08	WDR3 first beta-propeller domain
Beta-prop_THOC3	PF25174	411 - 462	2.6e-08	THOC3 beta-propeller domain
WD40	PF00400	425 - 461	1e-07	WD domain, G-beta repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0011780)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G11920 AT4G22910
fragaria_vesca	FvH4_5g01220 FvH4_5g01220
malus_domestica	MD00G1131600.v1.1 MD04G1070400.v1.1 MD11G1180200.v1.1 MD13G1123600.v1.1
prunus_persica	Prupe.5G087300_v2.0.a1
pyrus_communis	pycom04g06380 pycom06g06850
rosa_chinensis	RchiOBHm_Chr7g0199871
rosa_laevigata	RLG00000003764
rosa_multiflora	Rmu_sc0002748.1_g000015
rosa_roxburghii	Rroxscaffold_3G00256420
rosa_rugosa	Rorug07G0056700
rosa_samantha	Rh7AG181900 Rh7CG191500
rosa_wichuraiana	Rw7G015960

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	400
AccI	GTMKAC	1 cut(s)	787
AccII	CGCG	5 cut(s)	73, 86, 160, 285, 870
AclWI	GGATC	2 cut(s)	2, 189
AcsI	RAATTY	2 cut(s)	238, 735
AcuI	CTGAAG	1 cut(s)	947
AcyI	GRCGYC	1 cut(s)	101
AfaI	GTAC	1 cut(s)	1229
AfiI	CCNNNNNNNGG	7 cut(s)	85, 339, 342, 484, 739, 952, 1455
AgsI	TTSAA	3 cut(s)	385, 857, 1002
AjiI	CACGTC	1 cut(s)	442
AjnI	CCWGG	2 cut(s)	479, 806
AluBI	AGCT	5 cut(s)	94, 1049, 1152, 1210, 1276
AluI	AGCT	5 cut(s)	94, 1049, 1152, 1210, 1276
Alw21I	GWGCWC	1 cut(s)	1212
Alw26I	GTCTC	3 cut(s)	92, 248, 386
AlwI	GGATC	2 cut(s)	2, 189
AoxI	GGCC	5 cut(s)	105, 298, 778, 804, 1291
ApeKI	GCWGC	7 cut(s)	43, 67, 415, 418, 688, 1037, 1152
ApoI	RAATTY	2 cut(s)	238, 735
ArsI	GACNNNNNNTTYG	4 cut(s)	230, 262, 969, 1001
Asp700I	GAANNNNTTC	1 cut(s)	374
AspLEI	GCGC	4 cut(s)	266, 287, 688, 872
AsuC2I	CCSGG	2 cut(s)	109, 397
AsuHPI	GGTGA	3 cut(s)	439, 481, 1317
AvaII	GGWCC	7 cut(s)	75, 88, 162, 402, 487, 1059, 1191
BaeI	ACNNNNGTAYC	2 cut(s)	1096, 1129
BanII	GRGCYC	1 cut(s)	1212
BbsI	GAAGAC	3 cut(s)	479, 555, 819
Bbv12I	GWGCWC	1 cut(s)	1212
BbvI	GCAGC	7 cut(s)	54, 55, 402, 405, 700, 1024, 1139
BccI	CCATC	5 cut(s)	173, 789, 1074, 1328, 1457
BceAI	ACGGC	4 cut(s)	139, 406, 822, 1025
BciT130I	CCWGG	2 cut(s)	481, 808
BciVI	GTATCC	1 cut(s)	1124
BcnI	CCSGG	2 cut(s)	109, 397
BcoDI	GTCTC	3 cut(s)	92, 248, 386
BfaI	CTAG	1 cut(s)	1439
BfmI	CTRYAG	1 cut(s)	1101
BfoI	RGCGCY	1 cut(s)	267
BfuI	GTATCC	1 cut(s)	1124
BglI	GCCNNNNNGGC	1 cut(s)	109
Bme1390I	CCNGG	4 cut(s)	109, 397, 481, 808
Bme18I	GGWCC	7 cut(s)	75, 88, 162, 402, 487, 1059, 1191
BmgBI	CACGTC	1 cut(s)	442
BmiI	GGNNCC	4 cut(s)	77, 301, 489, 1061
BmrFI	CCNGG	4 cut(s)	109, 397, 481, 808
BmsI	GCATC	6 cut(s)	505, 733, 755, 965, 1094, 1439
BoxI	GACNNNNGTC	2 cut(s)	907, 1343
BpiI	GAAGAC	3 cut(s)	479, 555, 819
BpmI	CTGGAG	3 cut(s)	116, 244, 829
Bpu10I	CCTNAGC	1 cut(s)	873
BpuEI	CTTGAG	1 cut(s)	1168
BpuMI	CCSGG	2 cut(s)	109, 397
BsaAI	YACGTR	1 cut(s)	791
BsaBI	GATNNNNATC	1 cut(s)	540
BsaHI	GRCGYC	1 cut(s)	101
BsaI	GGTCTC	2 cut(s)	248, 386
BsaJI	CCNNGG	2 cut(s)	71, 479
BsaWI	WCCGGW	3 cut(s)	378, 449, 466
BsaXI	ACNNNNNCTCC	4 cut(s)	242, 272, 1214, 1244
Bsc4I	CCNNNNNNNGG	7 cut(s)	85, 339, 342, 484, 739, 952, 1455
Bse118I	RCCGGY	3 cut(s)	103, 311, 520
Bse1I	ACTGG	2 cut(s)	721, 1168
Bse3DI	GCAATG	1 cut(s)	727
Bse8I	GATNNNNATC	1 cut(s)	540
BseBI	CCWGG	2 cut(s)	481, 808
BseDI	CCNNGG	2 cut(s)	71, 479
BseGI	GGATG	3 cut(s)	520, 748, 815
BseJI	GATNNNNATC	1 cut(s)	540
BseLI	CCNNNNNNNGG	7 cut(s)	85, 339, 342, 484, 739, 952, 1455
BseMI	GCAATG	1 cut(s)	727
BseMII	CTCAG	3 cut(s)	257, 887, 1418
BseNI	ACTGG	2 cut(s)	721, 1168
BseXI	GCAGC	7 cut(s)	54, 55, 402, 405, 700, 1024, 1139
BseYI	CCCAGC	2 cut(s)	188, 681
Bsh1236I	CGCG	5 cut(s)	73, 86, 160, 285, 870
Bsh1285I	CGRYCG	2 cut(s)	152, 340
BshFI	GGCC	5 cut(s)	107, 300, 780, 806, 1293
BsiEI	CGRYCG	2 cut(s)	152, 340
BsiHKAI	GWGCWC	1 cut(s)	1212
BslFI	GGGAC	3 cut(s)	473, 667, 1045
BslI	CCNNNNNNNGG	7 cut(s)	85, 339, 342, 484, 739, 952, 1455
BsmAI	GTCTC	3 cut(s)	92, 248, 386
BsmBI	CGTCTC	1 cut(s)	92
BsmFI	GGGAC	3 cut(s)	473, 667, 1045
BsmI	GAATGC	2 cut(s)	129, 619
BsnI	GGCC	5 cut(s)	107, 300, 780, 806, 1293
Bso31I	GGTCTC	2 cut(s)	248, 386
Bsp1286I	GDGCHC	1 cut(s)	1212
Bsp143I	GATC	5 cut(s)	7, 149, 194, 337, 494
BspANI	GGCC	5 cut(s)	107, 300, 780, 806, 1293
BspCNI	CTCAG	3 cut(s)	256, 886, 1417
BspFNI	CGCG	5 cut(s)	73, 86, 160, 285, 870
BspLI	GGNNCC	4 cut(s)	77, 301, 489, 1061
BspMAI	CTGCAG	1 cut(s)	1105
BspPI	GGATC	2 cut(s)	2, 189
BspTNI	GGTCTC	2 cut(s)	248, 386
BsrDI	GCAATG	1 cut(s)	727
BsrFI	RCCGGY	3 cut(s)	103, 311, 520
BsrI	ACTGG	2 cut(s)	721, 1168
BssAI	RCCGGY	3 cut(s)	103, 311, 520
BssECI	CCNNGG	2 cut(s)	71, 479
BssMI	GATC	5 cut(s)	7, 149, 194, 337, 494
BssNI	GRCGYC	1 cut(s)	101
Bst2UI	CCWGG	2 cut(s)	481, 808
Bst4CI	ACNGT	3 cut(s)	599, 947, 1025
Bst6I	CTCTTC	1 cut(s)	299
BstACI	GRCGYC	1 cut(s)	101
BstAPI	GCANNNNNTGC	1 cut(s)	415
BstBAI	YACGTR	1 cut(s)	791
BstC8I	GCNNGC	3 cut(s)	105, 313, 1278
BstDEI	CTNAG	5 cut(s)	243, 636, 873, 1370, 1404
BstDSI	CCRYGG	1 cut(s)	71
BstEII	GGTNACC	1 cut(s)	445
BstF5I	GGATG	3 cut(s)	520, 748, 815
BstFNI	CGCG	5 cut(s)	73, 86, 160, 285, 870
BstH2I	RGCGCY	1 cut(s)	267
BstHHI	GCGC	4 cut(s)	266, 287, 688, 872
BstKTI	GATC	5 cut(s)	10, 152, 197, 340, 497
BstMAI	GTCTC	3 cut(s)	92, 248, 386
BstMBI	GATC	5 cut(s)	7, 149, 194, 337, 494
BstMCI	CGRYCG	2 cut(s)	152, 340
BstNI	CCWGG	2 cut(s)	481, 808
BstPAI	GACNNNNGTC	2 cut(s)	907, 1343
BstPI	GGTNACC	1 cut(s)	445
BstSCI	CCNGG	4 cut(s)	107, 395, 479, 806
BstSFI	CTRYAG	1 cut(s)	1101
BstSNI	TACGTA	1 cut(s)	791
BstUI	CGCG	5 cut(s)	73, 86, 160, 285, 870
BstV1I	GCAGC	7 cut(s)	54, 55, 402, 405, 700, 1024, 1139
BstV2I	GAAGAC	3 cut(s)	479, 555, 819
BstX2I	RGATCY	1 cut(s)	194
BstXI	CCANNNNNNTGG	1 cut(s)	1073
BstYI	RGATCY	1 cut(s)	194
BsuI	GTATCC	1 cut(s)	1124
BsuRI	GGCC	5 cut(s)	107, 300, 780, 806, 1293
BtgI	CCRYGG	1 cut(s)	71
BtrI	CACGTC	1 cut(s)	442
BtsCI	GGATG	3 cut(s)	520, 748, 815
BtsIMutI	CAGTG	1 cut(s)	1161
Cac8I	GCNNGC	3 cut(s)	105, 313, 1278
CfoI	GCGC	4 cut(s)	266, 287, 688, 872
Cfr10I	RCCGGY	3 cut(s)	103, 311, 520
Cfr42I	CCGCGG	1 cut(s)	74
CseI	GACGC	2 cut(s)	73, 109
Csp6I	GTAC	1 cut(s)	1228
CviAII	CATG	6 cut(s)	406, 820, 1056, 1073, 1156, 1434
CviQI	GTAC	1 cut(s)	1228
DdeI	CTNAG	5 cut(s)	243, 636, 873, 1370, 1404
DpnI	GATC	5 cut(s)	9, 151, 196, 339, 496
DpnII	GATC	5 cut(s)	7, 149, 194, 337, 494
DrdI	GACNNNNNNGTC	1 cut(s)	400
DseDI	GACNNNNNNGTC	1 cut(s)	400
Eam1104I	CTCTTC	1 cut(s)	299
EarI	CTCTTC	1 cut(s)	299
Ecl136II	GAGCTC	1 cut(s)	1210
Eco105I	TACGTA	1 cut(s)	791
Eco24I	GRGCYC	1 cut(s)	1212
Eco31I	GGTCTC	2 cut(s)	248, 386
Eco32I	GATATC	1 cut(s)	1259
Eco47I	GGWCC	7 cut(s)	75, 88, 162, 402, 487, 1059, 1191
Eco53kI	GAGCTC	1 cut(s)	1210
Eco57I	CTGAAG	1 cut(s)	947
Eco91I	GGTNACC	1 cut(s)	445
EcoICRI	GAGCTC	1 cut(s)	1210
EcoO109I	RGGNCCY	1 cut(s)	487
EcoO65I	GGTNACC	1 cut(s)	445
EcoRI	GAATTC	1 cut(s)	238
EcoRII	CCWGG	2 cut(s)	479, 806
EcoRV	GATATC	1 cut(s)	1259
EcoT22I	ATGCAT	1 cut(s)	748
EcoT38I	GRGCYC	1 cut(s)	1212
Esp3I	CGTCTC	1 cut(s)	92
FaeI	CATG	6 cut(s)	409, 823, 1059, 1076, 1159, 1437
FaqI	GGGAC	3 cut(s)	473, 667, 1045
FatI	CATG	6 cut(s)	405, 819, 1055, 1072, 1155, 1433
FauI	CCCGC	4 cut(s)	336, 366, 684, 956
FblI	GTMKAC	1 cut(s)	787
FokI	GGATG	3 cut(s)	527, 755, 802
FriOI	GRGCYC	1 cut(s)	1212
FspBI	CTAG	1 cut(s)	1439
GlaI	GCGC	4 cut(s)	265, 286, 687, 871
GsaI	CCCAGC	2 cut(s)	192, 685
GsuI	CTGGAG	3 cut(s)	116, 244, 829
HaeII	RGCGCY	1 cut(s)	267
HaeIII	GGCC	5 cut(s)	107, 300, 780, 806, 1293
HgaI	GACGC	2 cut(s)	73, 109
HhaI	GCGC	4 cut(s)	266, 287, 688, 872
Hin1I	GRCGYC	1 cut(s)	101
Hin1II	CATG	6 cut(s)	409, 823, 1059, 1076, 1159, 1437
Hin6I	GCGC	4 cut(s)	264, 285, 686, 870
HinP1I	GCGC	4 cut(s)	264, 285, 686, 870
HincII	GTYRAC	1 cut(s)	136
HindII	GTYRAC	1 cut(s)	136
HinfI	GANTC	3 cut(s)	319, 547, 991
HphI	GGTGA	3 cut(s)	439, 481, 1317
Hpy166II	GTNNAC	3 cut(s)	136, 788, 923
Hpy188I	TCNGA	7 cut(s)	175, 250, 546, 913, 1242, 1407, 1427
Hpy188III	TCNNGA	4 cut(s)	385, 875, 900, 1123
Hpy8I	GTNNAC	3 cut(s)	136, 788, 923
Hpy99I	CGWCG	4 cut(s)	67, 160, 238, 254
HpyAV	CCTTC	2 cut(s)	478, 1401
HpyCH4III	ACNGT	3 cut(s)	599, 947, 1025
HpyCH4IV	ACGT	3 cut(s)	132, 441, 790
HpyCH4V	TGCA	9 cut(s)	409, 518, 529, 746, 1055, 1085, 1103, 1155, 1179
HpyF3I	CTNAG	5 cut(s)	243, 636, 873, 1370, 1404
HpySE526I	ACGT	3 cut(s)	132, 441, 790
Hsp92I	GRCGYC	1 cut(s)	101
Hsp92II	CATG	6 cut(s)	409, 823, 1059, 1076, 1159, 1437
HspAI	GCGC	4 cut(s)	264, 285, 686, 870
KroI	GCCGGC	2 cut(s)	103, 311
KroNI	GCCGGC	2 cut(s)	105, 313
KspI	CCGCGG	1 cut(s)	74
Kzo9I	GATC	5 cut(s)	7, 149, 194, 337, 494
LmnI	GCTCC	2 cut(s)	1352, 1427
Lsp1109I	GCAGC	7 cut(s)	54, 55, 402, 405, 700, 1024, 1139
LweI	GCATC	6 cut(s)	505, 733, 755, 965, 1094, 1439
MaeI	CTAG	1 cut(s)	1439
MaeII	ACGT	3 cut(s)	132, 441, 790
MaeIII	GTNAC	4 cut(s)	445, 631, 839, 1345
MalI	GATC	5 cut(s)	9, 151, 196, 339, 496
MboI	GATC	5 cut(s)	7, 149, 194, 337, 494
MfeI	CAATTG	1 cut(s)	1341
MflI	RGATCY	1 cut(s)	194
MhlI	GDGCHC	1 cut(s)	1212
MluCI	AATT	3 cut(s)	238, 735, 1341
MmeI	TCCRAC	1 cut(s)	273
MnlI	CCTC	8 cut(s)	90, 268, 302, 333, 753, 769, 907, 1088
Mph1103I	ATGCAT	1 cut(s)	748
MreI	CGCCGGCG	1 cut(s)	311
MroNI	GCCGGC	2 cut(s)	103, 311
MroXI	GAANNNNTTC	1 cut(s)	374
MslI	CAYNNNNRTG	1 cut(s)	1071
MspA1I	CMGCKG	3 cut(s)	73, 94, 691
MspR9I	CCNGG	4 cut(s)	109, 397, 481, 808
MunI	CAATTG	1 cut(s)	1341
Mva1269I	GAATGC	2 cut(s)	129, 619
MvaI	CCWGG	2 cut(s)	481, 808
MvnI	CGCG	5 cut(s)	73, 86, 160, 285, 870
NaeI	GCCGGC	2 cut(s)	105, 313
NciI	CCSGG	2 cut(s)	109, 397
NdeII	GATC	5 cut(s)	7, 149, 194, 337, 494
NgoMIV	GCCGGC	2 cut(s)	103, 311
NlaIII	CATG	6 cut(s)	409, 823, 1059, 1076, 1159, 1437
NlaIV	GGNNCC	4 cut(s)	77, 301, 489, 1061
NmuCI	GTSAC	3 cut(s)	445, 839, 1345
NsiI	ATGCAT	1 cut(s)	748
PcsI	WCGNNNNNNNCGW	1 cut(s)	419
PctI	GAATGC	2 cut(s)	129, 619
PdiI	GCCGGC	2 cut(s)	105, 313
PdmI	GAANNNNTTC	1 cut(s)	374
PfeI	GAWTC	3 cut(s)	319, 547, 991
PflFI	GACNNNGTC	1 cut(s)	443
Ple19I	CGATCG	2 cut(s)	152, 340
Ppu21I	YACGTR	1 cut(s)	791
PpuMI	RGGWCCY	1 cut(s)	487
PshAI	GACNNNNGTC	2 cut(s)	907, 1343
Psp124BI	GAGCTC	1 cut(s)	1212
Psp5II	RGGWCCY	1 cut(s)	487
Psp6I	CCWGG	2 cut(s)	479, 806
PspEI	GGTNACC	1 cut(s)	445
PspFI	CCCAGC	2 cut(s)	188, 681
PspGI	CCWGG	2 cut(s)	479, 806
PspN4I	GGNNCC	4 cut(s)	77, 301, 489, 1061
PspPPI	RGGWCCY	1 cut(s)	487
PstI	CTGCAG	1 cut(s)	1105
PsuI	RGATCY	1 cut(s)	194
PsyI	GACNNNGTC	1 cut(s)	443
PvuI	CGATCG	2 cut(s)	152, 340
PvuII	CAGCTG	1 cut(s)	94
RsaI	GTAC	1 cut(s)	1229
RsaNI	GTAC	1 cut(s)	1228
RseI	CAYNNNNRTG	1 cut(s)	1071
SacI	GAGCTC	1 cut(s)	1212
SacII	CCGCGG	1 cut(s)	74
Sau3AI	GATC	5 cut(s)	7, 149, 194, 337, 494
ScrFI	CCNGG	4 cut(s)	109, 397, 481, 808
SduI	GDGCHC	1 cut(s)	1212
SfaNI	GCATC	6 cut(s)	505, 733, 755, 965, 1094, 1439
SfcI	CTRYAG	1 cut(s)	1101
Sfr303I	CCGCGG	1 cut(s)	74
SgrAI	CRCCGGYG	1 cut(s)	311
SgrBI	CCGCGG	1 cut(s)	74
SinI	GGWCC	7 cut(s)	75, 88, 162, 402, 487, 1059, 1191
SmiMI	CAYNNNNRTG	1 cut(s)	1071
SmlI	CTYRAG	1 cut(s)	1147
SmoI	CTYRAG	1 cut(s)	1147
SnaBI	TACGTA	1 cut(s)	791
Sse9I	AATT	3 cut(s)	238, 735, 1341
SspMI	CTAG	1 cut(s)	1439
SstI	GAGCTC	1 cut(s)	1212
StyD4I	CCNGG	4 cut(s)	107, 395, 479, 806
TaaI	ACNGT	3 cut(s)	599, 947, 1025
TaiI	ACGT	3 cut(s)	135, 444, 793
TaqI	TCGA	2 cut(s)	117, 219
TaqII	GACCGA	1 cut(s)	1122
TasI	AATT	3 cut(s)	238, 735, 1341
TauI	GCSGC	4 cut(s)	58, 61, 73, 290
TfiI	GAWTC	3 cut(s)	319, 547, 991
TscAI	CASTG	1 cut(s)	1168
TseFI	GTSAC	3 cut(s)	445, 839, 1345
TseI	GCWGC	7 cut(s)	43, 67, 415, 418, 688, 1037, 1152
Tsp45I	GTSAC	3 cut(s)	445, 839, 1345
TspDTI	ATGAA	6 cut(s)	172, 368, 446, 804, 1061, 1377
TspGWI	ACGGA	2 cut(s)	1106, 1347
TspRI	CASTG	1 cut(s)	1168
Tth111I	GACNNNGTC	1 cut(s)	443
VpaK11BI	GGWCC	7 cut(s)	75, 88, 162, 402, 487, 1059, 1191
XapI	RAATTY	2 cut(s)	238, 735
XmiI	GTMKAC	1 cut(s)	787
XmnI	GAANNNNTTC	1 cut(s)	374
XspI	CTAG	1 cut(s)	1439
Zsp2I	ATGCAT	1 cut(s)	748

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.