Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0011780

Physical Location & Seq

Genomic Coordinates Reverse (-)

9629877 .. 9634633

Gene Structure 4757 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1070400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1434 bp

ATGGAGGATACCACTCCCACAACCCCATCGACTCCGGCTCTGCTCCGCACTCCTCAGGCACTACATTCCCTAACCCCATCTCGGCACATCGACCGCCTGATCAACTACAACCACCACCAATCGCCGTCCAGGACCATCTACTCCGATAGGTTCATCCCCAGTAGATCCGGTTCCAACTTCCCCCTCTTCGACATCTCCAATACTCCATCCGAGGGCCGCGATGACTCGTCAAGCGCCTACGGTACTCTCCTACGCGCTGCCCTCTTTGGACCTGACTCCGCCGGCGTCGTTCCTCCGGCGACTCCCGAGAAAAGGAGCGCGATTCAGATGAACCCGCCCAGCCCCAACATCTTCCGGTTCAAGACCGAGACTCGCCGGTCAATGCACTCGCTTTCCCCTTTTGGGTTCGATGATGTGGTCACCGGAGTTCACCATAGCCCCGTCAAGGCTCCTCGGAAGGTTCCCCGGTCGCCTTATAAGGTTTTGGATGCACCTGCTTTGCAGGACGATTTCTATCTGAATCTTGTCGATTGGTCTTCGCACAATGTGCTTGCTGTTGGGTTGGGAAATTGTGTTTATTTATGGAATGCTTGTAGTAGCAAGGTAACTAAGTTATGTGACTTGGGGATTGATGATAGTGTCTGTTCCGTTGGCTGGGCTCAACGTGGAACTCATCTTGCGGTTGGAACTAGCAATGGGAAAGTCCAAATATGGGATGCATCCCGGTGTAGGAGGGTAAGAACCATGGAGGGCCACCGACTACGCGTTGGCGCCTTAGCCTGGAGTTCATCTATGCTGTCTTCTGGTAGCCGGGACAAAACTATTCTTCAACGTGATATTCGTGCTCAGGACGATTTTGTTAGCAAACTTTCCGGACACAAGTCAGAGGTTTGTGGACTGAAATGGTCATATGATAATCGTGAGCTGGCATCAGGCGGGAATGATAACAGGCTTTTTGTTTGGAATCAACATTCAACTCAACCGGTGCTCAAATACTGTGAGCATACTGCAGCTGTGAAAGCCATTGCATGGTCTCCACATCAGCATGGGCTTCTTGCATCTGGAGGAGGAACAGCAGACCGATGTATTCGTTTCTGGAATACTACCACCAACTCACACTTGAGCTGCATGGACACTGGAAGTCAGGTTTGCAATCTTGTGTGGTCTAAAAATGTCAATGAACTAGTCAGCACCCATGGATACTCCCAAAATCAAATAATAGTTTGGAGATATCCCACCATGTCAAAGCTGGCAACTCTTACGGGCCATTCATACAGAGTTCTTTATCTTGCCATCTCACCTGATGGACAGACAATTGTCACTGGAGCAGGAGATGAAACACTAAGATTTTGGAATGTGTTTCCTTCCCCCAAGTCCCAGAACACGGAAAGTGAAATTGGAGCATCATCGCTTGGAAGAACTGTTATCCGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0000904 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006275 GO:0006725 GO:0006807 GO:0007049 GO:0008150 GO:0008152 GO:0008361 GO:0009058 GO:0009059 GO:0009653 GO:0009825 GO:0009888 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010026 GO:0010073 GO:0010074 GO:0010090 GO:0010091 GO:0010492 GO:0010556 GO:0010557 GO:0010564 GO:0010604 GO:0016043 GO:0016049 GO:0019219 GO:0019222 GO:0019827 GO:0022402 GO:0030154 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0032535 GO:0032875 GO:0032877 GO:0032989 GO:0034641 GO:0034645 GO:0040007 GO:0042023 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044424 GO:0044464 GO:0044786 GO:0045740 GO:0045787 GO:0045935 GO:0046483 GO:0048468 GO:0048507 GO:0048518 GO:0048522 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051052 GO:0051054 GO:0051171 GO:0051173 GO:0051726 GO:0060255 GO:0065007 GO:0065008 GO:0071704 GO:0071840 GO:0080090 GO:0090066 GO:0090068 GO:0090304 GO:0090329 GO:0090558 GO:0090626 GO:0098727 GO:1901360 GO:1901576 GO:2000105 GO:2000112

KEGG Pathways

Metabolic & Signaling

ko04110 ko04111 ko04120 ko04914 map04110 map04111 map04120 map04914

Pfam Domains

Protein Families

ANAPC4_WD40 WD40

Protein Analysis

478

Amino Acids

52.62

Weight (kDa)

9.19

Isoelectric Point (pI)

48.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WD40_CDC20-Fz	PF24807	162 - 451	2.8e-128	CDC20/Fizzy WD40 domain
WD40_WDHD1_1st	PF24817	174 - 263	4e-08	WDHD1 first WD40 domain
Beta-prop_EML_2	PF23414	180 - 347	6.8e-17	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR19_1st	PF23389	182 - 275	2.1e-12	WDR19 first beta-propeller
Beta-prop_WDR3_1st	PF25173	183 - 283	2.2e-13	WDR3 first beta-propeller domain
Beta-prop_RIG_2nd	PF23775	192 - 440	3.8e-06	RIG second beta-propeller
WDR55	PF24796	208 - 456	8.4e-12	WDR55
Beta-prop_WDR5	PF25175	211 - 377	4.3e-24	WDR5 beta-propeller domain
Beta-prop_THOC3	PF25174	212 - 284	1.4e-09	THOC3 beta-propeller domain
WD40_MABP1-WDR62_2nd	PF24782	213 - 452	1e-15	MABP1/WDR62 second WD40 domain
Beta-prop_TEP1_2nd	PF25047	214 - 386	1.2e-11	TEP-1 second beta-propeller
Beta-prop_WDR36-Utp21_1st	PF25171	226 - 366	8.3e-07	WDR36/Utp21 first beta-propeller
WD40_Prp19	PF24814	264 - 452	8.7e-18	Prp19 WD40 domain
WD40_WDHD1_1st	PF24817	283 - 352	1.5e-06	WDHD1 first WD40 domain
Beta-prop_WDR3_1st	PF25173	286 - 397	5.4e-15	WDR3 first beta-propeller domain
WD40	PF00400	287 - 322	4.7e-07	WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1	PF24105	288 - 373	2.3e-10	CAF1B/HIR1 beta-propeller domain
WD40_Gbeta	PF25391	288 - 456	1.8e-12	G protein beta WD-40 repeat protein
Beta-prop_THOC3	PF25174	293 - 399	1.9e-19	THOC3 beta-propeller domain
Beta-prop_Aladin	PF25460	294 - 453	5.1e-08	Aladin seven-bladed propeller
Beta-prop_EML	PF23409	332 - 454	6.7e-11	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR36-Utp21_2nd	PF25168	335 - 453	1.1e-06	WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR5	PF25175	335 - 453	1.8e-13	WDR5 beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	339 - 461	8.3e-09	WDR3 second beta-propeller domain
Beta-prop_EML_2	PF23414	341 - 454	2.9e-06	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st	PF25173	382 - 453	2.7e-08	WDR3 first beta-propeller domain
Beta-prop_THOC3	PF25174	402 - 453	6e-08	THOC3 beta-propeller domain
WD40	PF00400	416 - 452	1.3e-07	WD domain, G-beta repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0011780)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G11920 AT4G22910
fragaria_vesca	FvH4_5g01220 FvH4_5g01220
malus_domestica	MD00G1131600.v1.1 MD04G1070400.v1.1 MD11G1180200.v1.1 MD13G1123600.v1.1
prunus_persica	Prupe.5G087300_v2.0.a1
pyrus_communis	pycom04g06380 pycom06g06850
rosa_chinensis	RchiOBHm_Chr7g0199871
rosa_laevigata	RLG00000003764
rosa_multiflora	Rmu_sc0002748.1_g000015
rosa_roxburghii	Rroxscaffold_3G00256420
rosa_rugosa	Rorug07G0056700
rosa_samantha	Rh7AG181900 Rh7CG191500
rosa_wichuraiana	Rw7G015960

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	477
AarI	CACCTGC	1 cut(s)	502
AasI	GACNNNNNNGTC	1 cut(s)	376
Acc36I	ACCTGC	1 cut(s)	502
AccB1I	GGYRCC	1 cut(s)	770
AccB7I	CCANNNNNTGG	1 cut(s)	1029
AccII	CGCG	4 cut(s)	219, 255, 320, 765
AccIII	TCCGGA	1 cut(s)	872
AciI	CCGC	7 cut(s)	46, 94, 217, 279, 335, 680, 936
AclWI	GGATC	1 cut(s)	159
AcyI	GRCGYC	2 cut(s)	285, 771
AdeI	CACNNNGTG	1 cut(s)	547
AfaI	GTAC	1 cut(s)	244
AflIII	ACRYGT	1 cut(s)	763
AgeI	ACCGGT	1 cut(s)	982
AgsI	TTSAA	3 cut(s)	361, 830, 975
AhlI	ACTAGT	1 cut(s)	1183
AjnI	CCWGG	2 cut(s)	128, 779
AjuI	GAANNNNNNNTTGG	2 cut(s)	1380, 1412
AluBI	AGCT	4 cut(s)	925, 1013, 1125, 1249
AluI	AGCT	4 cut(s)	925, 1013, 1125, 1249
Alw21I	GWGCWC	2 cut(s)	847, 990
Alw26I	GTCTC	2 cut(s)	362, 1038
AlwI	GGATC	1 cut(s)	159
Ama87I	CYCGRG	1 cut(s)	305
Aor13HI	TCCGGA	1 cut(s)	872
AoxI	GGCC	3 cut(s)	214, 751, 1264
ApeKI	GCWGC	3 cut(s)	257, 1010, 1125
AsiGI	ACCGGT	1 cut(s)	982
AspLEI	GCGC	4 cut(s)	236, 257, 320, 773
AspS9I	GGNCC	5 cut(s)	132, 214, 269, 751, 1264
AsuC2I	CCSGG	3 cut(s)	466, 724, 812
AsuHPI	GGTGA	3 cut(s)	412, 422, 1290
AvaI	CYCGRG	1 cut(s)	305
AvaII	GGWCC	2 cut(s)	132, 269
AxyI	CCTNAGG	1 cut(s)	54
BanI	GGYRCC	1 cut(s)	770
BanII	GRGCYC	1 cut(s)	661
BbsI	GAAGAC	2 cut(s)	528, 792
Bbv12I	GWGCWC	2 cut(s)	847, 990
BbvI	GCAGC	3 cut(s)	244, 1022, 1112
BccI	CCATC	6 cut(s)	34, 85, 143, 214, 1298, 1301
BceAI	ACGGC	1 cut(s)	109
BciT130I	CCWGG	2 cut(s)	130, 781
BciVI	GTATCC	1 cut(s)	1193
BclI	TGATCA	1 cut(s)	99
BcnI	CCSGG	3 cut(s)	466, 724, 812
BcoDI	GTCTC	2 cut(s)	362, 1038
BcuI	ACTAGT	1 cut(s)	1183
BfaI	CTAG	2 cut(s)	690, 1184
BfmI	CTRYAG	1 cut(s)	1008
BfoI	RGCGCY	2 cut(s)	237, 774
BfuAI	ACCTGC	1 cut(s)	502
BfuI	GTATCC	1 cut(s)	1193
BisI	GCNGC	4 cut(s)	217, 258, 1011, 1126
BlsI	GCNGC	4 cut(s)	218, 259, 1012, 1127
Bme1390I	CCNGG	5 cut(s)	130, 466, 724, 781, 812
Bme18I	GGWCC	2 cut(s)	132, 269
BmeT110I	CYCGRG	1 cut(s)	305
BmgT120I	GGNCC	5 cut(s)	132, 214, 269, 751, 1264
BmiI	GGNNCC	4 cut(s)	172, 450, 462, 772
BmrFI	CCNGG	5 cut(s)	130, 466, 724, 781, 812
BmrI	ACTGGG	1 cut(s)	153
BmsI	GCATC	6 cut(s)	478, 706, 728, 938, 1067, 1412
BmuI	ACTGGG	1 cut(s)	153
BoxI	GACNNNNGTC	2 cut(s)	880, 1316
BpiI	GAAGAC	2 cut(s)	528, 792
BpmI	CTGGAG	3 cut(s)	802, 1083, 1344
Bpu10I	CCTNAGC	2 cut(s)	775, 846
BpuEI	CTTGAG	1 cut(s)	1141
BpuMI	CCSGG	3 cut(s)	466, 724, 812
BsaBI	GATNNNNATC	1 cut(s)	513
BsaHI	GRCGYC	2 cut(s)	285, 771
BsaI	GGTCTC	1 cut(s)	1038
BsaJI	CCNNGG	5 cut(s)	210, 452, 464, 744, 1195
BsaWI	WCCGGW	6 cut(s)	167, 354, 422, 872, 982, 1428
Bse118I	RCCGGY	3 cut(s)	281, 375, 982
Bse1I	ACTGG	3 cut(s)	159, 1141, 1327
Bse21I	CCTNAGG	1 cut(s)	54
Bse3DI	GCAATG	2 cut(s)	700, 1023
Bse8I	GATNNNNATC	1 cut(s)	513
BseAI	TCCGGA	1 cut(s)	872
BseBI	CCWGG	2 cut(s)	130, 781
BseDI	CCNNGG	5 cut(s)	210, 452, 464, 744, 1195
BseGI	GGATG	5 cut(s)	153, 206, 493, 719, 721
BseJI	GATNNNNATC	1 cut(s)	513
BseMI	GCAATG	2 cut(s)	700, 1023
BseMII	CTCAG	2 cut(s)	68, 860
BseNI	ACTGG	3 cut(s)	159, 1141, 1327
BseRI	GAGGAG	3 cut(s)	42, 441, 1080
BseXI	GCAGC	3 cut(s)	244, 1022, 1112
BseYI	CCCAGC	2 cut(s)	338, 654
Bsh1236I	CGCG	4 cut(s)	219, 255, 320, 765
Bsh1285I	CGRYCG	2 cut(s)	94, 470
BshFI	GGCC	3 cut(s)	216, 753, 1266
BshNI	GGYRCC	1 cut(s)	770
BshTI	ACCGGT	1 cut(s)	982
BsiEI	CGRYCG	2 cut(s)	94, 470
BsiHKAI	GWGCWC	2 cut(s)	847, 990
BsiHKCI	CYCGRG	1 cut(s)	305
BslFI	GGGAC	2 cut(s)	827, 1360
BsmAI	GTCTC	2 cut(s)	362, 1038
BsmFI	GGGAC	2 cut(s)	827, 1360
BsmI	GAATGC	1 cut(s)	592
BsnI	GGCC	3 cut(s)	216, 753, 1266
Bso31I	GGTCTC	1 cut(s)	1038
BsoBI	CYCGRG	1 cut(s)	305
Bsp1286I	GDGCHC	3 cut(s)	661, 847, 990
Bsp13I	TCCGGA	1 cut(s)	872
Bsp143I	GATC	2 cut(s)	99, 164
Bsp19I	CCATGG	2 cut(s)	744, 1195
BspACI	CCGC	7 cut(s)	46, 94, 217, 279, 335, 680, 936
BspANI	GGCC	3 cut(s)	216, 753, 1266
BspCNI	CTCAG	2 cut(s)	67, 859
BspEI	TCCGGA	1 cut(s)	872
BspFNI	CGCG	4 cut(s)	219, 255, 320, 765
BspLI	GGNNCC	4 cut(s)	172, 450, 462, 772
BspMAI	CTGCAG	1 cut(s)	1012
BspMI	ACCTGC	1 cut(s)	502
BspPI	GGATC	1 cut(s)	159
BspT107I	GGYRCC	1 cut(s)	770
BspTNI	GGTCTC	1 cut(s)	1038
BsrDI	GCAATG	2 cut(s)	700, 1023
BsrFI	RCCGGY	3 cut(s)	281, 375, 982
BsrI	ACTGG	3 cut(s)	159, 1141, 1327
BssAI	RCCGGY	3 cut(s)	281, 375, 982
BssECI	CCNNGG	5 cut(s)	210, 452, 464, 744, 1195
BssMI	GATC	2 cut(s)	99, 164
BssNI	GRCGYC	2 cut(s)	285, 771
BssT1I	CCWWGG	2 cut(s)	744, 1195
Bst2UI	CCWGG	2 cut(s)	130, 781
Bst4CI	ACNGT	3 cut(s)	242, 998, 1423
Bst6I	CTCTTC	1 cut(s)	191
BstACI	GRCGYC	2 cut(s)	285, 771
BstAPI	GCANNNNNTGC	1 cut(s)	547
BstC8I	GCNNGC	4 cut(s)	283, 552, 927, 1251
BstDEI	CTNAG	5 cut(s)	54, 609, 775, 846, 1343
BstDSI	CCRYGG	2 cut(s)	744, 1195
BstEII	GGTNACC	1 cut(s)	418
BstF5I	GGATG	5 cut(s)	153, 206, 493, 719, 721
BstFNI	CGCG	4 cut(s)	219, 255, 320, 765
BstH2I	RGCGCY	2 cut(s)	237, 774
BstHHI	GCGC	4 cut(s)	236, 257, 320, 773
BstKTI	GATC	2 cut(s)	102, 167
BstMAI	GTCTC	2 cut(s)	362, 1038
BstMBI	GATC	2 cut(s)	99, 164
BstMCI	CGRYCG	2 cut(s)	94, 470
BstMWI	GCNNNNNNNGC	2 cut(s)	547, 1019
BstNI	CCWGG	2 cut(s)	130, 781
BstPAI	GACNNNNGTC	2 cut(s)	880, 1316
BstPI	GGTNACC	1 cut(s)	418
BstSCI	CCNGG	5 cut(s)	128, 464, 722, 779, 810
BstSFI	CTRYAG	1 cut(s)	1008
BstUI	CGCG	4 cut(s)	219, 255, 320, 765
BstV1I	GCAGC	3 cut(s)	244, 1022, 1112
BstV2I	GAAGAC	2 cut(s)	528, 792
BstX2I	RGATCY	1 cut(s)	164
BstYI	RGATCY	1 cut(s)	164
Bsu36I	CCTNAGG	1 cut(s)	54
BsuI	GTATCC	1 cut(s)	1193
BsuRI	GGCC	3 cut(s)	216, 753, 1266
BtgI	CCRYGG	2 cut(s)	744, 1195
BtgZI	GCGATG	2 cut(s)	234, 1392
BtsCI	GGATG	5 cut(s)	153, 206, 493, 719, 721
BtsIMutI	CAGTG	2 cut(s)	1134, 1320
BveI	ACCTGC	1 cut(s)	502
Cac8I	GCNNGC	4 cut(s)	283, 552, 927, 1251
CfoI	GCGC	4 cut(s)	236, 257, 320, 773
Cfr10I	RCCGGY	3 cut(s)	281, 375, 982
Cfr13I	GGNCC	5 cut(s)	132, 214, 269, 751, 1264
CseI	GACGC	1 cut(s)	274
Csp6I	GTAC	1 cut(s)	243
CspAI	ACCGGT	1 cut(s)	982
CviAII	CATG	6 cut(s)	745, 1029, 1046, 1129, 1196, 1240
CviQI	GTAC	1 cut(s)	243
DdeI	CTNAG	5 cut(s)	54, 609, 775, 846, 1343
DinI	GGCGCC	1 cut(s)	772
DpnI	GATC	2 cut(s)	101, 166
DpnII	GATC	2 cut(s)	99, 164
DraIII	CACNNNGTG	1 cut(s)	547
DrdI	GACNNNNNNGTC	1 cut(s)	376
DseDI	GACNNNNNNGTC	1 cut(s)	376
Eam1104I	CTCTTC	1 cut(s)	191
EarI	CTCTTC	1 cut(s)	191
EciI	GGCGGA	1 cut(s)	268
Eco130I	CCWWGG	2 cut(s)	744, 1195
Eco24I	GRGCYC	1 cut(s)	661
Eco31I	GGTCTC	1 cut(s)	1038
Eco32I	GATATC	1 cut(s)	1232
Eco47I	GGWCC	2 cut(s)	132, 269
Eco81I	CCTNAGG	1 cut(s)	54
Eco88I	CYCGRG	1 cut(s)	305
Eco91I	GGTNACC	1 cut(s)	418
EcoO65I	GGTNACC	1 cut(s)	418
EcoRII	CCWGG	2 cut(s)	128, 779
EcoRV	GATATC	1 cut(s)	1232
EcoT14I	CCWWGG	2 cut(s)	744, 1195
EcoT22I	ATGCAT	1 cut(s)	721
EcoT38I	GRGCYC	1 cut(s)	661
EgeI	GGCGCC	1 cut(s)	772
EheI	GGCGCC	1 cut(s)	772
ErhI	CCWWGG	2 cut(s)	744, 1195
FaeI	CATG	6 cut(s)	748, 1032, 1049, 1132, 1199, 1243
FaqI	GGGAC	2 cut(s)	827, 1360
FatI	CATG	6 cut(s)	744, 1028, 1045, 1128, 1195, 1239
FauI	CCCGC	2 cut(s)	342, 929
FauNDI	CATATG	1 cut(s)	910
FbaI	TGATCA	1 cut(s)	99
Fnu4HI	GCNGC	4 cut(s)	217, 258, 1011, 1126
FokI	GGATG	5 cut(s)	140, 193, 500, 706, 728
FriOI	GRGCYC	1 cut(s)	661
Fsp4HI	GCNGC	4 cut(s)	217, 258, 1011, 1126
FspBI	CTAG	2 cut(s)	690, 1184
GlaI	GCGC	4 cut(s)	235, 256, 319, 772
GluI	GCNGC	4 cut(s)	217, 258, 1011, 1126
GsaI	CCCAGC	2 cut(s)	342, 658
GsuI	CTGGAG	3 cut(s)	802, 1083, 1344
HaeII	RGCGCY	2 cut(s)	237, 774
HaeIII	GGCC	3 cut(s)	216, 753, 1266
HgaI	GACGC	1 cut(s)	274
HhaI	GCGC	4 cut(s)	236, 257, 320, 773
Hin1I	GRCGYC	2 cut(s)	285, 771
Hin1II	CATG	6 cut(s)	748, 1032, 1049, 1132, 1199, 1243
Hin6I	GCGC	4 cut(s)	234, 255, 318, 771
HinP1I	GCGC	4 cut(s)	234, 255, 318, 771
HinfI	GANTC	8 cut(s)	31, 224, 275, 301, 322, 370, 520, 964
HphI	GGTGA	3 cut(s)	412, 422, 1290
Hpy166II	GTNNAC	2 cut(s)	430, 896
Hpy188I	TCNGA	6 cut(s)	145, 211, 327, 456, 519, 886
Hpy188III	TCNNGA	7 cut(s)	305, 361, 848, 873, 920, 1062, 1096
Hpy8I	GTNNAC	2 cut(s)	430, 896
Hpy99I	CGWCG	1 cut(s)	290
HpyAV	CCTTC	2 cut(s)	451, 1374
HpyCH4III	ACNGT	3 cut(s)	242, 998, 1423
HpyCH4IV	ACGT	2 cut(s)	664, 832
HpyCH4V	TGCA	9 cut(s)	385, 491, 502, 719, 1010, 1028, 1058, 1128, 1152
HpyF10VI	GCNNNNNNNGC	2 cut(s)	547, 1019
HpyF3I	CTNAG	5 cut(s)	54, 609, 775, 846, 1343
HpySE526I	ACGT	2 cut(s)	664, 832
Hsp92I	GRCGYC	2 cut(s)	285, 771
Hsp92II	CATG	6 cut(s)	748, 1032, 1049, 1132, 1199, 1243
HspAI	GCGC	4 cut(s)	234, 255, 318, 771
KasI	GGCGCC	1 cut(s)	770
Kpn2I	TCCGGA	1 cut(s)	872
KroI	GCCGGC	1 cut(s)	281
KroNI	GCCGGC	1 cut(s)	283
Ksp22I	TGATCA	1 cut(s)	99
Kzo9I	GATC	2 cut(s)	99, 164
LmnI	GCTCC	5 cut(s)	48, 315, 454, 1325, 1400
Lsp1109I	GCAGC	3 cut(s)	244, 1022, 1112
LweI	GCATC	6 cut(s)	478, 706, 728, 938, 1067, 1412
MaeI	CTAG	2 cut(s)	690, 1184
MaeII	ACGT	2 cut(s)	664, 832
MaeIII	GTNAC	4 cut(s)	418, 604, 617, 1318
MalI	GATC	2 cut(s)	101, 166
MboI	GATC	2 cut(s)	99, 164
MboII	GAAGA	6 cut(s)	178, 343, 528, 792, 818, 1428
MfeI	CAATTG	1 cut(s)	1314
MflI	RGATCY	1 cut(s)	164
MhlI	GDGCHC	3 cut(s)	661, 847, 990
MluCI	AATT	3 cut(s)	568, 1314, 1395
MluI	ACGCGT	1 cut(s)	763
Mly113I	GGCGCC	1 cut(s)	771
MlyI	GAGTC	5 cut(s)	25, 218, 269, 295, 364
MmeI	TCCRAC	2 cut(s)	198, 664
Mph1103I	ATGCAT	1 cut(s)	721
MreI	CGCCGGCG	1 cut(s)	281
MroI	TCCGGA	1 cut(s)	872
MroNI	GCCGGC	1 cut(s)	281
MslI	CAYNNNNRTG	2 cut(s)	724, 1044
MspA1I	CMGCKG	1 cut(s)	1013
MspR9I	CCNGG	5 cut(s)	130, 466, 724, 781, 812
MunI	CAATTG	1 cut(s)	1314
Mva1269I	GAATGC	1 cut(s)	592
MvaI	CCWGG	2 cut(s)	130, 781
MvnI	CGCG	4 cut(s)	219, 255, 320, 765
MwoI	GCNNNNNNNGC	2 cut(s)	547, 1019
NaeI	GCCGGC	1 cut(s)	283
NarI	GGCGCC	1 cut(s)	771
NciI	CCSGG	3 cut(s)	466, 724, 812
NcoI	CCATGG	2 cut(s)	744, 1195
NdeI	CATATG	1 cut(s)	910
NdeII	GATC	2 cut(s)	99, 164
NgoMIV	GCCGGC	1 cut(s)	281
NlaIII	CATG	6 cut(s)	748, 1032, 1049, 1132, 1199, 1243
NlaIV	GGNNCC	4 cut(s)	172, 450, 462, 772
NmeAIII	GCCGAG	1 cut(s)	61
NmuCI	GTSAC	3 cut(s)	418, 617, 1318
NsiI	ATGCAT	1 cut(s)	721
PaqCI	CACCTGC	1 cut(s)	502
PcsI	WCGNNNNNNNCGW	1 cut(s)	838
PctI	GAATGC	1 cut(s)	592
PdiI	GCCGGC	1 cut(s)	283
PfeI	GAWTC	3 cut(s)	322, 520, 964
PflMI	CCANNNNNTGG	1 cut(s)	1029
PfoI	TCCNGGA	1 cut(s)	128
PinAI	ACCGGT	1 cut(s)	982
PkrI	GCNGC	4 cut(s)	218, 259, 1012, 1127
PleI	GAGTC	5 cut(s)	25, 218, 269, 295, 364
PluTI	GGCGCC	1 cut(s)	774
PpsI	GAGTC	5 cut(s)	25, 218, 269, 295, 364
PshAI	GACNNNNGTC	2 cut(s)	880, 1316
PsiI	TTATAA	1 cut(s)	477
Psp6I	CCWGG	2 cut(s)	128, 779
PspEI	GGTNACC	1 cut(s)	418
PspFI	CCCAGC	2 cut(s)	338, 654
PspGI	CCWGG	2 cut(s)	128, 779
PspN4I	GGNNCC	4 cut(s)	172, 450, 462, 772
PspPI	GGNCC	5 cut(s)	132, 214, 269, 751, 1264
PsrI	GAACNNNNNNTAC	2 cut(s)	154, 186
PstI	CTGCAG	1 cut(s)	1012
PsuI	RGATCY	1 cut(s)	164
PvuII	CAGCTG	1 cut(s)	1013
RsaI	GTAC	1 cut(s)	244
RsaNI	GTAC	1 cut(s)	243
RseI	CAYNNNNRTG	2 cut(s)	724, 1044
SatI	GCNGC	4 cut(s)	217, 258, 1011, 1126
Sau3AI	GATC	2 cut(s)	99, 164
Sau96I	GGNCC	5 cut(s)	132, 214, 269, 751, 1264
SchI	GAGTC	5 cut(s)	25, 218, 269, 295, 364
ScrFI	CCNGG	5 cut(s)	130, 466, 724, 781, 812
SduI	GDGCHC	3 cut(s)	661, 847, 990
SfaNI	GCATC	6 cut(s)	478, 706, 728, 938, 1067, 1412
SfcI	CTRYAG	1 cut(s)	1008
SfoI	GGCGCC	1 cut(s)	772
SgrAI	CRCCGGYG	1 cut(s)	281
SinI	GGWCC	2 cut(s)	132, 269
SmiMI	CAYNNNNRTG	2 cut(s)	724, 1044
SmlI	CTYRAG	1 cut(s)	1120
SmoI	CTYRAG	1 cut(s)	1120
SpeI	ACTAGT	1 cut(s)	1183
Sse9I	AATT	3 cut(s)	568, 1314, 1395
SsiI	CCGC	7 cut(s)	46, 94, 217, 279, 335, 680, 936
SspDI	GGCGCC	1 cut(s)	770
SspMI	CTAG	2 cut(s)	690, 1184
StyD4I	CCNGG	5 cut(s)	128, 464, 722, 779, 810
StyI	CCWWGG	2 cut(s)	744, 1195
TaaI	ACNGT	3 cut(s)	242, 998, 1423
TaiI	ACGT	2 cut(s)	667, 835
TaqI	TCGA	5 cut(s)	29, 90, 189, 408, 528
TaqII	GACCGA	2 cut(s)	380, 1095
TasI	AATT	3 cut(s)	568, 1314, 1395
TauI	GCSGC	1 cut(s)	219
TfiI	GAWTC	3 cut(s)	322, 520, 964
TscAI	CASTG	2 cut(s)	1141, 1327
TseFI	GTSAC	3 cut(s)	418, 617, 1318
TseI	GCWGC	3 cut(s)	257, 1010, 1125
Tsp45I	GTSAC	3 cut(s)	418, 617, 1318
TspDTI	ATGAA	6 cut(s)	142, 344, 777, 1194, 1260, 1350
TspGWI	ACGGA	2 cut(s)	637, 1400
TspRI	CASTG	2 cut(s)	1141, 1327
Van91I	CCANNNNNTGG	1 cut(s)	1029
VpaK11BI	GGWCC	2 cut(s)	132, 269
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1246
XspI	CTAG	2 cut(s)	690, 1184
Zsp2I	ATGCAT	1 cut(s)	721

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.