FvH4_5g06720

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0006447
Physical Location & Seq
Forward (+)
3962314 .. 3964811
2498 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g06720.t5

Sequence Viewer

Length: 1239 bp
ATGGCAATGCCTTCCGGCCCCTCTCTCTCTCTCCTCGTCTTCCTCTTCTCTCTCCTCACCCTAACCCTCATTGCTGTTTCCTCTTCACCAGTTCAAGATCCTGAACTTGTTGTACAACAAGTACAAAGGAGCATCTATAATGCCTCAACTACCCGGAGGAAGCTGGGATACTTGTCGTGTGGGACCGGAAACCCGATTGACGACTGCTGGCGGTGCGACCCGAATTGGGAGAAGAACCGCCAGAGGCTGGCCGACTGTGCAATTGGGTTCGGCAAAAACGCCATTGGCGGCAGGGACGGGAGGATTTACGTGGTGACTGACTCAGGTGACGACGACCCTGTCACGCCTAAGCCAGGTACTCTACGACATGCCGTCATCCAGGACGAGCCTTTATGGATCATTTTCGAGCGCGACATGTCGATAAAGTTGAAGGAGGAGCTGATCATGAACTCTTTCAAGACCCTCGACGGCCGAGGAGCCAGCGTCCACATCTCCGGCGGACCATGCATTACGGTCCAGTACGTGACCAACATTATCATACATGGACTCAACATACACGATTGCAAGCCCGGAGGTAACGCTATGGTTCGTGACTCCCCGAACCACTTCGGGTGGAGAACAATATCAGACGGTGACGGTGTTTCTATCTTCGGCGGGAGCCATGTTTGGGTGGATCACTGCTCGTTGTCTAACTGCGCCGACGGCCTCGTCGACGCCATCCATGGCTCCACCGCGATAACGATTTCGAACAATTACATGACGCACCACGATAAGGTCATGTTGCTGGGACATAGCGATTCCTATACCCAGGACAAGAACATGCAGGTCACTATTGCCTTCAATCACTTTGGAGAAGGTCTTGTTCAAAGGATGCCAAGATGTAGGCATGGATATTTCCATGTGGTGAACAATGACTACACCCATTGGGAGATGTATGCCATTGGAGGGAGTGCAAACCCTACCATCAATAGCCAAGGGAATAGATTTGCTGCACCAGACATAAGATTCAGTAAAGAGGTGACGAAACATGAGGATGCGCCCGAAAGTGAGTGGAGGAATTGGAACTGGAGGTCCGAGGGTGACATGTTGATAAACGGTGCATTTTTCACGGCCTCTGGTGCTGGAGCTTCTTCAAGCTATGCAAAAGCTTCAAGCTTGGGAGCAAGGCCATCTTCACTCGTGGGTTCCATCACCACAACTTCTGGTGCACTCAATTGCCGAAAGGGTTCTCGTTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

413

Amino Acids

45.06

Weight (kDa)

6.59

Isoelectric Point (pI)

33.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectate_lyase_4 PF00544 209 - 331 1.1e-19 Pectate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 338, 707
Acc36I ACCTGC 1 cut(s) 814
AccB7I CCANNNNNTGG 1 cut(s) 247
AccI GTMKAC 1 cut(s) 711
AccII CGCG 2 cut(s) 411, 734
AciI CCGC 6 cut(s) 211, 238, 288, 498, 654, 732
AclWI GGATC 3 cut(s) 92, 404, 681
AcoI YGGCCR 2 cut(s) 249, 469
AcyI GRCGYC 1 cut(s) 714
AfaI GTAC 4 cut(s) 114, 123, 358, 521
AfiI CCNNNNNNNGG 6 cut(s) 226, 247, 353, 667, 772, 945
AflIII ACRYGT 2 cut(s) 414, 1083
AgsI TTSAA 7 cut(s) 95, 430, 457, 841, 866, 1134, 1152
AhdI GACNNNNNGTC 1 cut(s) 371
AjnI CCWGG 3 cut(s) 352, 378, 807
AloI GAACNNNNNNTCC 2 cut(s) 1055, 1087
AluBI AGCT 6 cut(s) 163, 439, 1127, 1137, 1148, 1155
AluI AGCT 6 cut(s) 163, 439, 1127, 1137, 1148, 1155
Alw21I GWGCWC 1 cut(s) 1210
Alw44I GTGCAC 1 cut(s) 1206
AlwI GGATC 3 cut(s) 92, 404, 681
AlwNI CAGNNNCTG 1 cut(s) 247
AoxI GGCC 6 cut(s) 16, 249, 469, 703, 1110, 1166
ApaLI GTGCAC 1 cut(s) 1206
ApeKI GCWGC 1 cut(s) 989
Asp700I GAANNNNTTC 3 cut(s) 452, 605, 1225
AspLEI GCGC 3 cut(s) 411, 698, 1039
AspS9I GGNCC 5 cut(s) 17, 183, 500, 514, 1071
AsuC2I CCSGG 2 cut(s) 154, 570
AsuHPI GGTGA 9 cut(s) 49, 78, 325, 338, 644, 916, 1030, 1091, 1183
AsuII TTCGAA 1 cut(s) 746
AvaII GGWCC 4 cut(s) 183, 500, 514, 1071
BaeGI GKGCMC 1 cut(s) 1210
BarI GAAGNNNNNNTAC 2 cut(s) 152, 184
BauI CACGAG 1 cut(s) 1178
BbsI GAAGAC 1 cut(s) 31
Bbv12I GWGCWC 1 cut(s) 1210
BbvI GCAGC 1 cut(s) 976
BccI CCATC 4 cut(s) 725, 971, 1177, 1196
BceAI ACGGC 4 cut(s) 356, 484, 718, 1125
BciT130I CCWGG 3 cut(s) 354, 380, 809
BciVI GTATCC 1 cut(s) 161
BclI TGATCA 1 cut(s) 441
BcnI CCSGG 2 cut(s) 154, 570
BfuAI ACCTGC 1 cut(s) 814
BfuI GTATCC 1 cut(s) 161
BisI GCNGC 2 cut(s) 289, 990
BlsI GCNGC 2 cut(s) 290, 991
Bme1390I CCNGG 5 cut(s) 154, 354, 380, 570, 809
Bme18I GGWCC 4 cut(s) 183, 500, 514, 1071
BmeRI GACNNNNNGTC 1 cut(s) 371
BmgT120I GGNCC 5 cut(s) 17, 183, 500, 514, 1071
BmiI GGNNCC 6 cut(s) 19, 184, 478, 659, 727, 1186
BmrFI CCNGG 5 cut(s) 154, 354, 380, 570, 809
BmsI GCATC 3 cut(s) 141, 861, 1024
BpiI GAAGAC 1 cut(s) 31
BpmI CTGGAG 2 cut(s) 1087, 1143
Bpu10I CCTNAGC 1 cut(s) 348
Bpu14I TTCGAA 1 cut(s) 746
BpuMI CCSGG 2 cut(s) 154, 570
BsaAI YACGTR 2 cut(s) 310, 523
BsaHI GRCGYC 1 cut(s) 714
BsaJI CCNNGG 5 cut(s) 472, 721, 807, 973, 1074
BsaWI WCCGGW 1 cut(s) 185
BsaXI ACNNNNNCTCC 2 cut(s) 468, 498
Bsc4I CCNNNNNNNGG 6 cut(s) 226, 247, 353, 667, 772, 945
Bse1I ACTGG 3 cut(s) 89, 517, 1070
Bse3DI GCAATG 2 cut(s) 12, 69
BseBI CCWGG 3 cut(s) 354, 380, 809
BseDI CCNNGG 5 cut(s) 472, 721, 807, 973, 1074
BseGI GGATG 4 cut(s) 375, 717, 876, 1039
BseLI CCNNNNNNNGG 6 cut(s) 226, 247, 353, 667, 772, 945
BseMI GCAATG 2 cut(s) 12, 69
BseMII CTCAG 1 cut(s) 336
BseNI ACTGG 3 cut(s) 89, 517, 1070
BseRI GAGGAG 4 cut(s) 23, 44, 449, 489
BseSI GKGCMC 1 cut(s) 1210
BseX3I CGGCCG 1 cut(s) 469
BseXI GCAGC 1 cut(s) 976
BseYI CCCAGC 2 cut(s) 163, 784
BsgI GTGCAG 1 cut(s) 975
Bsh1236I CGCG 2 cut(s) 411, 734
Bsh1285I CGRYCG 1 cut(s) 472
BshFI GGCC 6 cut(s) 18, 251, 471, 705, 1112, 1168
BsiEI CGRYCG 1 cut(s) 472
BsiHKAI GWGCWC 1 cut(s) 1210
BsiSI CCGG 5 cut(s) 15, 154, 186, 495, 570
BslFI GGGAC 3 cut(s) 196, 308, 801
BslI CCNNNNNNNGG 6 cut(s) 226, 247, 353, 667, 772, 945
BsmFI GGGAC 3 cut(s) 196, 308, 801
BsnI GGCC 6 cut(s) 18, 251, 471, 705, 1112, 1168
Bsp119I TTCGAA 1 cut(s) 746
Bsp1286I GDGCHC 1 cut(s) 1210
Bsp1407I TGTACA 1 cut(s) 112
Bsp143I GATC 4 cut(s) 97, 396, 441, 673
Bsp19I CCATGG 1 cut(s) 721
BspACI CCGC 6 cut(s) 211, 238, 288, 498, 654, 732
BspANI GGCC 6 cut(s) 18, 251, 471, 705, 1112, 1168
BspCNI CTCAG 1 cut(s) 335
BspFNI CGCG 2 cut(s) 411, 734
BspHI TCATGA 1 cut(s) 444
BspLI GGNNCC 6 cut(s) 19, 184, 478, 659, 727, 1186
BspMI ACCTGC 1 cut(s) 814
BspPI GGATC 3 cut(s) 92, 404, 681
BspT104I TTCGAA 1 cut(s) 746
BsrDI GCAATG 2 cut(s) 12, 69
BsrGI TGTACA 1 cut(s) 112
BsrI ACTGG 3 cut(s) 89, 517, 1070
BssECI CCNNGG 5 cut(s) 472, 721, 807, 973, 1074
BssMI GATC 4 cut(s) 97, 396, 441, 673
BssNI GRCGYC 1 cut(s) 714
BssSI CACGAG 1 cut(s) 1178
BssT1I CCWWGG 2 cut(s) 721, 973
Bst2BI CACGAG 1 cut(s) 1178
Bst2UI CCWGG 3 cut(s) 354, 380, 809
Bst4CI ACNGT 5 cut(s) 257, 514, 632, 638, 1097
Bst6I CTCTTC 2 cut(s) 50, 88
BstACI GRCGYC 1 cut(s) 714
BstAUI TGTACA 1 cut(s) 112
BstBAI YACGTR 2 cut(s) 310, 523
BstBI TTCGAA 1 cut(s) 746
BstC8I GCNNGC 4 cut(s) 209, 249, 481, 566
BstDEI CTNAG 2 cut(s) 322, 348
BstDSI CCRYGG 1 cut(s) 721
BstENI CCTNNNNNAGG 1 cut(s) 351
BstF5I GGATG 4 cut(s) 375, 717, 876, 1039
BstFNI CGCG 2 cut(s) 411, 734
BstHHI GCGC 3 cut(s) 411, 698, 1039
BstKTI GATC 4 cut(s) 100, 399, 444, 676
BstMBI GATC 4 cut(s) 97, 396, 441, 673
BstMCI CGRYCG 1 cut(s) 472
BstMWI GCNNNNNNNGC 5 cut(s) 213, 257, 504, 702, 1118
BstNI CCWGG 3 cut(s) 354, 380, 809
BstNSI RCATGY 4 cut(s) 371, 418, 823, 1087
BstSCI CCNGG 5 cut(s) 152, 352, 378, 568, 807
BstSLI GKGCMC 1 cut(s) 1210
BstUI CGCG 2 cut(s) 411, 734
BstV1I GCAGC 1 cut(s) 976
BstV2I GAAGAC 1 cut(s) 31
BstX2I RGATCY 1 cut(s) 97
BstYI RGATCY 1 cut(s) 97
BstZI CGGCCG 1 cut(s) 469
BsuI GTATCC 1 cut(s) 161
BsuRI GGCC 6 cut(s) 18, 251, 471, 705, 1112, 1168
BtgI CCRYGG 1 cut(s) 721
BtsCI GGATG 4 cut(s) 375, 717, 876, 1039
BtsI GCAGTG 1 cut(s) 676
BtsIMutI CAGTG 1 cut(s) 676
BveI ACCTGC 1 cut(s) 814
Cac8I GCNNGC 4 cut(s) 209, 249, 481, 566
CaiI CAGNNNCTG 1 cut(s) 247
CciI TCATGA 1 cut(s) 444
CfoI GCGC 3 cut(s) 411, 698, 1039
Cfr13I GGNCC 5 cut(s) 17, 183, 500, 514, 1071
CseI GACGC 3 cut(s) 472, 722, 769
Csp6I GTAC 4 cut(s) 113, 122, 357, 520
CviQI GTAC 4 cut(s) 113, 122, 357, 520
DdeI CTNAG 2 cut(s) 322, 348
DpnI GATC 4 cut(s) 99, 398, 443, 675
DpnII GATC 4 cut(s) 97, 396, 441, 673
DrdI GACNNNNNNGTC 2 cut(s) 338, 707
DriI GACNNNNNGTC 1 cut(s) 371
DseDI GACNNNNNNGTC 2 cut(s) 338, 707
EaeI YGGCCR 2 cut(s) 249, 469
EagI CGGCCG 1 cut(s) 469
Eam1104I CTCTTC 2 cut(s) 50, 88
Eam1105I GACNNNNNGTC 1 cut(s) 371
EarI CTCTTC 2 cut(s) 50, 88
EciI GGCGGA 1 cut(s) 513
EclXI CGGCCG 1 cut(s) 469
Eco130I CCWWGG 2 cut(s) 721, 973
Eco47I GGWCC 4 cut(s) 183, 500, 514, 1071
Eco52I CGGCCG 1 cut(s) 469
EcoNI CCTNNNNNAGG 1 cut(s) 351
EcoRII CCWGG 3 cut(s) 352, 378, 807
EcoT14I CCWWGG 2 cut(s) 721, 973
EcoT22I ATGCAT 1 cut(s) 509
ErhI CCWWGG 2 cut(s) 721, 973
FalI AAGNNNNNCTT 2 cut(s) 1156, 1188
FaqI GGGAC 3 cut(s) 196, 308, 801
FauI CCCGC 1 cut(s) 647
FbaI TGATCA 1 cut(s) 441
FblI GTMKAC 1 cut(s) 711
Fnu4HI GCNGC 2 cut(s) 289, 990
FokI GGATG 4 cut(s) 362, 704, 883, 1046
Fsp4HI GCNGC 2 cut(s) 289, 990
GlaI GCGC 3 cut(s) 410, 697, 1038
GluI GCNGC 2 cut(s) 289, 990
GsaI CCCAGC 2 cut(s) 167, 788
GsuI CTGGAG 2 cut(s) 1087, 1143
HaeIII GGCC 6 cut(s) 18, 251, 471, 705, 1112, 1168
HapII CCGG 5 cut(s) 15, 154, 186, 495, 570
HgaI GACGC 3 cut(s) 472, 722, 769
HhaI GCGC 3 cut(s) 411, 698, 1039
Hin1I GRCGYC 1 cut(s) 714
Hin6I GCGC 3 cut(s) 409, 696, 1037
HinP1I GCGC 3 cut(s) 409, 696, 1037
HincII GTYRAC 1 cut(s) 712
HindII GTYRAC 1 cut(s) 712
HindIII AAGCTT 2 cut(s) 1146, 1153
HinfI GANTC 5 cut(s) 320, 546, 593, 797, 1005
HpaII CCGG 5 cut(s) 15, 154, 186, 495, 570
HphI GGTGA 9 cut(s) 49, 78, 325, 338, 644, 916, 1030, 1091, 1183
Hpy166II GTNNAC 4 cut(s) 487, 712, 907, 1208
Hpy188I TCNGA 2 cut(s) 628, 1075
Hpy188III TCNNGA 5 cut(s) 95, 101, 445, 457, 590
Hpy8I GTNNAC 4 cut(s) 487, 712, 907, 1208
Hpy99I CGWCG 5 cut(s) 335, 470, 704, 713, 716
HpyAV CCTTC 4 cut(s) 21, 424, 847, 848
HpyCH4III ACNGT 5 cut(s) 257, 514, 632, 638, 1097
HpyCH4IV ACGT 2 cut(s) 309, 522
HpyCH4V TGCA 9 cut(s) 260, 507, 564, 823, 953, 992, 1100, 1142, 1208
HpyF10VI GCNNNNNNNGC 5 cut(s) 213, 257, 504, 702, 1118
HpyF3I CTNAG 2 cut(s) 322, 348
HpySE526I ACGT 2 cut(s) 309, 522
Hsp92I GRCGYC 1 cut(s) 714
HspAI GCGC 3 cut(s) 409, 696, 1037
Ksp22I TGATCA 1 cut(s) 441
Kzo9I GATC 4 cut(s) 97, 396, 441, 673
LmnI GCTCC 7 cut(s) 129, 436, 476, 657, 731, 1124, 1160
Lsp1109I GCAGC 1 cut(s) 976
LweI GCATC 3 cut(s) 141, 861, 1024
MaeII ACGT 2 cut(s) 309, 522
MalI GATC 4 cut(s) 99, 398, 443, 675
MboI GATC 4 cut(s) 97, 396, 441, 673
MboII GAAGA 7 cut(s) 31, 37, 75, 244, 640, 1122, 1164
MfeI CAATTG 2 cut(s) 261, 1213
MflI RGATCY 1 cut(s) 97
MhlI GDGCHC 1 cut(s) 1210
MluCI AATT 5 cut(s) 223, 261, 751, 1057, 1213
MlyI GAGTC 3 cut(s) 314, 540, 587
Mph1103I ATGCAT 1 cut(s) 509
MroXI GAANNNNTTC 3 cut(s) 452, 605, 1225
MslI CAYNNNNRTG 1 cut(s) 1032
MspI CCGG 5 cut(s) 15, 154, 186, 495, 570
MspR9I CCNGG 5 cut(s) 154, 354, 380, 570, 809
MunI CAATTG 2 cut(s) 261, 1213
MvaI CCWGG 3 cut(s) 354, 380, 809
MvnI CGCG 2 cut(s) 411, 734
MwoI GCNNNNNNNGC 5 cut(s) 213, 257, 504, 702, 1118
NciI CCSGG 2 cut(s) 154, 570
NcoI CCATGG 1 cut(s) 721
NdeII GATC 4 cut(s) 97, 396, 441, 673
NlaIV GGNNCC 6 cut(s) 19, 184, 478, 659, 727, 1186
NmeAIII GCCGAG 1 cut(s) 497
NmuCI GTSAC 9 cut(s) 313, 326, 340, 523, 590, 632, 826, 1018, 1079
NsiI ATGCAT 1 cut(s) 509
NspI RCATGY 4 cut(s) 371, 418, 823, 1087
NspV TTCGAA 1 cut(s) 746
PagI TCATGA 1 cut(s) 444
PciI ACATGT 2 cut(s) 414, 1083
PcsI WCGNNNNNNNCGW 1 cut(s) 708
PdmI GAANNNNTTC 3 cut(s) 452, 605, 1225
PfeI GAWTC 2 cut(s) 797, 1005
PflFI GACNNNGTC 1 cut(s) 338
PflMI CCANNNNNTGG 1 cut(s) 247
PfoI TCCNGGA 1 cut(s) 378
PkrI GCNGC 2 cut(s) 290, 991
PleI GAGTC 3 cut(s) 314, 540, 587
PpsI GAGTC 3 cut(s) 314, 540, 587
Ppu21I YACGTR 2 cut(s) 310, 523
PscI ACATGT 2 cut(s) 414, 1083
Psp6I CCWGG 3 cut(s) 352, 378, 807
PspFI CCCAGC 2 cut(s) 163, 784
PspGI CCWGG 3 cut(s) 352, 378, 807
PspN4I GGNNCC 6 cut(s) 19, 184, 478, 659, 727, 1186
PspPI GGNCC 5 cut(s) 17, 183, 500, 514, 1071
PsrI GAACNNNNNNTAC 4 cut(s) 96, 128, 899, 931
PstNI CAGNNNCTG 1 cut(s) 247
PsuI RGATCY 1 cut(s) 97
PsyI GACNNNGTC 1 cut(s) 338
RsaI GTAC 4 cut(s) 114, 123, 358, 521
RsaNI GTAC 4 cut(s) 113, 122, 357, 520
RseI CAYNNNNRTG 1 cut(s) 1032
SalI GTCGAC 1 cut(s) 710
SatI GCNGC 2 cut(s) 289, 990
Sau3AI GATC 4 cut(s) 97, 396, 441, 673
Sau96I GGNCC 5 cut(s) 17, 183, 500, 514, 1071
SchI GAGTC 3 cut(s) 314, 540, 587
ScrFI CCNGG 5 cut(s) 154, 354, 380, 570, 809
SduI GDGCHC 1 cut(s) 1210
SfaNI GCATC 3 cut(s) 141, 861, 1024
SfuI TTCGAA 1 cut(s) 746
SgrDI CGTCGACG 1 cut(s) 710
SinI GGWCC 4 cut(s) 183, 500, 514, 1071
SmiMI CAYNNNNRTG 1 cut(s) 1032
Sse9I AATT 5 cut(s) 223, 261, 751, 1057, 1213
SsiI CCGC 6 cut(s) 211, 238, 288, 498, 654, 732
StyD4I CCNGG 5 cut(s) 152, 352, 378, 568, 807
StyI CCWWGG 2 cut(s) 721, 973
TaaI ACNGT 5 cut(s) 257, 514, 632, 638, 1097
TaiI ACGT 2 cut(s) 312, 525
TaqI TCGA 5 cut(s) 405, 419, 465, 711, 746
TasI AATT 5 cut(s) 223, 261, 751, 1057, 1213
TatI WGTACW 2 cut(s) 112, 121
TauI GCSGC 1 cut(s) 291
TfiI GAWTC 2 cut(s) 797, 1005
TscAI CASTG 1 cut(s) 683
TseFI GTSAC 9 cut(s) 313, 326, 340, 523, 590, 632, 826, 1018, 1079
TseI GCWGC 1 cut(s) 989
Tsp45I GTSAC 9 cut(s) 313, 326, 340, 523, 590, 632, 826, 1018, 1079
TspDTI ATGAA 1 cut(s) 461
TspRI CASTG 1 cut(s) 683
Tth111I GACNNNGTC 1 cut(s) 338
Van91I CCANNNNNTGG 1 cut(s) 247
VneI GTGCAC 1 cut(s) 1206
VpaK11BI GGWCC 4 cut(s) 183, 500, 514, 1071
XagI CCTNNNNNAGG 1 cut(s) 351
XceI RCATGY 4 cut(s) 371, 418, 823, 1087
XmiI GTMKAC 1 cut(s) 711
XmnI GAANNNNTTC 3 cut(s) 452, 605, 1225
Zsp2I ATGCAT 1 cut(s) 509
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.